BR112016013400A2 - métodos para modificar um genoma e para produzir um camundongo - Google Patents

métodos para modificar um genoma e para produzir um camundongo

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Publication number
BR112016013400A2
BR112016013400A2 BR112016013400A BR112016013400A BR112016013400A2 BR 112016013400 A2 BR112016013400 A2 BR 112016013400A2 BR 112016013400 A BR112016013400 A BR 112016013400A BR 112016013400 A BR112016013400 A BR 112016013400A BR 112016013400 A2 BR112016013400 A2 BR 112016013400A2
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Brazil
Prior art keywords
methods
modifying
human
cell
ltvec
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BR112016013400A
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English (en)
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BR112016013400B1 (pt
Inventor
Frendewey David
M Valenzuela David
D Yancopoulos George
KUNO Junko
Venus Lai Ka-Man
Auerbach Wojtek
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Regeneron Pharma
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Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of BR112016013400A2 publication Critical patent/BR112016013400A2/pt
Publication of BR112016013400B1 publication Critical patent/BR112016013400B1/pt

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    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N9/22—Ribonucleases RNAses, DNAses
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Abstract

composições e métodos são providos para modificar um locus genômico de interesse em uma célula eucariótica, uma célula de mamífero, uma célula humana ou uma célula não humana de mamífero usando um vetor direcionado amplo (ltvec) compreendendo várias sequências de ácido nucleico endógenas ou exógenas como descrito nesse documento. métodos adicionais combinam o uso do ltvec com um sistema crispr/cas. composições e métodos para gerar um animal não humano geneticamente modificado compreendendo uma ou mais modificações genéticas direcionadas em sua linha germinal são também providas.
BR112016013400-1A 2013-12-11 2014-10-15 Método in vitro para modificar um genoma em um lócus genômico de interesse em uma célula pluripotente BR112016013400B1 (pt)

Applications Claiming Priority (12)

Application Number Priority Date Filing Date Title
US201361914768P 2013-12-11 2013-12-11
US201462017416P 2014-06-26 2014-06-26
US62/017,416 2014-06-26
US201462029261P 2014-07-25 2014-07-25
US62/029,261 2014-07-25
US201462052906P 2014-09-19 2014-09-19
US62/052,906 2014-09-19
US201462059527P 2014-10-03 2014-10-03
US62/059,527 2014-10-03
US201462064384P 2014-10-15 2014-10-15
PCT/US2014/060788 WO2015088643A1 (en) 2013-12-11 2014-10-15 Methods and compositions for the targeted modification of a genome
US62/064,384 2014-10-15

Publications (2)

Publication Number Publication Date
BR112016013400A2 true BR112016013400A2 (pt) 2017-09-26
BR112016013400B1 BR112016013400B1 (pt) 2023-02-14

Family

ID=51830654

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BR112016013400-1A BR112016013400B1 (pt) 2013-12-11 2014-10-15 Método in vitro para modificar um genoma em um lócus genômico de interesse em uma célula pluripotente

Country Status (12)

Country Link
US (7) US9546384B2 (pt)
JP (3) JP6174811B2 (pt)
KR (2) KR101773782B1 (pt)
CN (2) CN105980568B (pt)
AU (3) AU2014360811B2 (pt)
BR (1) BR112016013400B1 (pt)
CA (1) CA2933433C (pt)
IL (1) IL245674B (pt)
MX (2) MX2016007654A (pt)
RU (2) RU2725520C2 (pt)
SG (1) SG10201700961TA (pt)
WO (1) WO2015088643A1 (pt)

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US11820997B2 (en) 2023-11-21
US20240052365A1 (en) 2024-02-15
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