BR112012030836B8 - Hospedeiro recombinante que compreende genes recombinantes para produção de esteviol ou glicosídeo de esteviol, método para produzir esteviol, glicosídeo de esteviol ou composição de glicosídeo de esteviol e método para sintetizar esteviol ou glicosídeo de esteviol - Google Patents

Hospedeiro recombinante que compreende genes recombinantes para produção de esteviol ou glicosídeo de esteviol, método para produzir esteviol, glicosídeo de esteviol ou composição de glicosídeo de esteviol e método para sintetizar esteviol ou glicosídeo de esteviol

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Publication number
BR112012030836B8
BR112012030836B8 BR112012030836A BR112012030836A BR112012030836B8 BR 112012030836 B8 BR112012030836 B8 BR 112012030836B8 BR 112012030836 A BR112012030836 A BR 112012030836A BR 112012030836 A BR112012030836 A BR 112012030836A BR 112012030836 B8 BR112012030836 B8 BR 112012030836B8
Authority
BR
Brazil
Prior art keywords
steviol
glycoside
steviol glycoside
producing
recombinant
Prior art date
Application number
BR112012030836A
Other languages
English (en)
Portuguese (pt)
Other versions
BR112012030836B1 (pt
BR112012030836A2 (pt
Inventor
Charlotte Blom
Halkjaer Hansen Esben
M Kishore Ganesh
Jens Houghton-Larsen
Jorgen Hansen
Dalgaard Mikkelsen Michael
Michael Motion
M Hicks Paula
Sabina Tavares
Original Assignee
Evolva Nutrition Inc
Evolva Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva Nutrition Inc, Evolva Inc filed Critical Evolva Nutrition Inc
Priority to BR122021005297-2A priority Critical patent/BR122021005297B1/pt
Priority to BR122021005304-9A priority patent/BR122021005304B1/pt
Priority to BR122021005283-2A priority patent/BR122021005283B1/pt
Priority to BR122021005287-5A priority patent/BR122021005287B1/pt
Publication of BR112012030836A2 publication Critical patent/BR112012030836A2/pt
Publication of BR112012030836B1 publication Critical patent/BR112012030836B1/pt
Publication of BR112012030836B8 publication Critical patent/BR112012030836B8/pt

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    • C12N9/1051—Hexosyltransferases (2.4.1)
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Families Citing this family (144)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8790730B2 (en) 2005-10-11 2014-07-29 Purecircle Usa Process for manufacturing a sweetener and use thereof
US8257948B1 (en) 2011-02-17 2012-09-04 Purecircle Usa Method of preparing alpha-glucosyl Stevia composition
US9107436B2 (en) 2011-02-17 2015-08-18 Purecircle Sdn Bhd Glucosylated steviol glycoside as a flavor modifier
US9386797B2 (en) 2011-02-17 2016-07-12 Purecircle Sdn Bhd Glucosyl stevia composition
US8318459B2 (en) 2011-02-17 2012-11-27 Purecircle Usa Glucosyl stevia composition
US9392799B2 (en) 2011-02-17 2016-07-19 Purecircle Sdn Bhd Glucosyl stevia composition
US8927241B2 (en) 2009-11-10 2015-01-06 Massachusetts Institute Of Technology Microbial engineering for the production of chemical and pharmaceutical products from the isoprenoid pathway
EP2594648B1 (en) 2009-11-10 2016-03-16 Massachusetts Institute of Technology Microbial engineering for the production of chemical and pharmaceutical products from the isoprenoid pathway
EP3461342A1 (en) 2009-11-12 2019-04-03 PureCircle USA Inc. Granulation of a stevia sweetener
US20150344512A1 (en) 2011-12-19 2015-12-03 Purecircle Usa Inc. Methods of purifying steviol glycosides and uses of the same
US10696706B2 (en) 2010-03-12 2020-06-30 Purecircle Usa Inc. Methods of preparing steviol glycosides and uses of the same
WO2011112892A1 (en) 2010-03-12 2011-09-15 Purecircle Usa Inc. High-purity steviol glycosides
EP2571991A1 (en) * 2010-05-20 2013-03-27 Evolva SA Method of producing isoprenoid compounds in yeast
CN105671108A (zh) 2010-06-02 2016-06-15 沃维公司 甜菊糖苷的重组生产
WO2012075030A1 (en) * 2010-11-30 2012-06-07 Massachusetts Institute Of Technology Microbial production of natural sweeteners, diterpenoid steviol glycosides
US9510611B2 (en) 2010-12-13 2016-12-06 Purecircle Sdn Bhd Stevia composition to improve sweetness and flavor profile
BR112013014589B1 (pt) 2010-12-13 2019-04-02 Purecircle Usa Inc. Método para preparar uma composição de rebaudiosídeo d altamente solúvel
WO2012177727A1 (en) 2011-06-20 2012-12-27 Purecircle Usa Inc. Stevia composition
US10362797B2 (en) 2011-02-10 2019-07-30 Purecircle Sdn Bhd Stevia composition
US9474296B2 (en) 2011-02-17 2016-10-25 Purecircle Sdn Bhd Glucosyl stevia composition
US11690391B2 (en) 2011-02-17 2023-07-04 Purecircle Sdn Bhd Glucosylated steviol glycoside as a flavor modifier
US9603373B2 (en) 2011-02-17 2017-03-28 Purecircle Sdn Bhd Glucosyl stevia composition
US9894922B2 (en) 2011-05-18 2018-02-20 Purecircle Sdn Bhd Glucosyl rebaudioside C
US10021899B2 (en) 2011-05-31 2018-07-17 Purecircle Sdn Bhd Stevia composition
ES2579827T3 (es) 2011-06-03 2016-08-17 Purecircle Usa Composición de estevia
US9771434B2 (en) 2011-06-23 2017-09-26 Purecircle Sdn Bhd Products from stevia rebaudiana
WO2013022989A2 (en) 2011-08-08 2013-02-14 Evolva Sa Recombinant production of steviol glycosides
US10480019B2 (en) 2011-08-10 2019-11-19 Purecircle Sdn Bhd Process for producing high-purity rubusoside
EP3811786A1 (en) 2011-09-07 2021-04-28 PureCircle USA Inc. Highly soluble stevia sweetener and method for producing thereof
CN103031283B (zh) * 2011-10-08 2015-07-08 成都华高瑞甜科技有限公司 甜叶菊酶vi及莱鲍迪苷a转化为莱鲍迪苷d的方法
WO2013076577A1 (en) 2011-11-23 2013-05-30 Evolva Sa Methods and materials for enzymatic synthesis of mogroside compounds
ES2787899T3 (es) 2011-12-19 2020-10-19 Purecircle Sdn Bhd Métodos para purificar glucósidos de esteviol
FR2984743B1 (fr) * 2011-12-23 2014-08-22 Oreal Utilisation cosmetique du steviol, d'un derive glycoside du steviol, ou un de leurs isomeres pour stimuler, restaurer ou reguler le metabolisme des cellules de la peau et des semi-muqueuses.
CN104203005A (zh) * 2012-01-23 2014-12-10 帝斯曼知识产权资产管理有限公司 二萜的生产
CA2867112C (en) * 2012-03-16 2021-04-20 Suntory Holdings Limited Steviol glucosyltransferases and genes encoding the same
US10113154B2 (en) * 2012-03-27 2018-10-30 Suntory Holdings Limited Method for producing steviol glycoside
US20130338348A1 (en) * 2012-03-30 2013-12-19 J.R. Simplot Company Steviol and steviol glycoside formation in plants
CN103974628B (zh) * 2012-05-22 2019-04-05 谱赛科有限责任公司 高纯度的甜菊醇糖苷
US9752174B2 (en) * 2013-05-28 2017-09-05 Purecircle Sdn Bhd High-purity steviol glycosides
TW201402599A (zh) 2012-05-30 2014-01-16 Suntory Holdings Ltd 甜菊糖苷糖苷轉化酵素及編碼該酵素之基因
EP2902410B1 (en) * 2012-09-29 2022-01-05 Sichuan Ingia Biosynthetic Co., Ltd. Method for producing stevioside compounds by microorganism
EP2929043A1 (en) 2012-12-04 2015-10-14 Evolva SA Methods and materials for biosynthesis of mogroside compounds
US20150342234A1 (en) * 2012-12-05 2015-12-03 Evolva Sa Steviol Glycoside Compositions Sensory Properties
SG11201505597SA (en) * 2013-02-06 2015-08-28 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
EP2954061B1 (en) * 2013-02-11 2023-11-22 Evolva SA Efficient production of steviol glycosides in recombinant hosts
KR101404728B1 (ko) * 2013-02-28 2014-06-09 씨제이제일제당 (주) 스테비오사이드로부터 리바우디오사이드 a를 제조하는 방법
EP2970354B1 (en) * 2013-03-15 2018-05-30 The Coca-Cola Company Steviol glycosides and their compositions
MX367033B (es) 2013-05-31 2019-08-02 Dsm Ip Assets Bv Microorganismos para la produccion de diterpeno.
BR112015029601A2 (pt) * 2013-05-31 2017-09-26 Dsm Ip Assets Bv produção de diterpeno extracelular
CN112220026A (zh) 2013-06-07 2021-01-15 谱赛科美国股份有限公司 含有选择的甜叶菊醇糖苷作为香、咸和甜度特征修饰物的甜叶菊提取物
US10952458B2 (en) 2013-06-07 2021-03-23 Purecircle Usa Inc Stevia extract containing selected steviol glycosides as flavor, salty and sweetness profile modifier
KR101559478B1 (ko) 2013-06-24 2015-10-13 한국생명공학연구원 효소전환법을 이용한 천연 고감미료의 제조방법
CN108064135A (zh) * 2013-07-15 2018-05-22 帝斯曼知识产权资产管理有限公司 二萜的生产
US10273519B2 (en) 2013-07-23 2019-04-30 Dsm Ip Assets B.V. Diterpene production in Yarrowia
CA2917615A1 (en) * 2013-07-31 2015-02-05 Dsm Ip Assets B.V. Steviol glycosides
WO2015014959A1 (en) * 2013-07-31 2015-02-05 Dsm Ip Assets B.V. Recovery of steviol glycosides
WO2015016393A1 (ja) * 2013-08-02 2015-02-05 サントリーホールディングス株式会社 ヘキセノール配糖体化酵素の利用方法
CN103397064B (zh) 2013-08-14 2015-04-15 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙m的方法
US20160215306A1 (en) * 2013-08-30 2016-07-28 Evolva Sa Method for producing modified resveratrol
AU2014342939B2 (en) * 2013-11-01 2018-03-22 Conagen Inc. Recombinant production of steviol glycosides
WO2015113569A1 (en) 2014-01-31 2015-08-06 University Of Copenhagen Biosynthesis of forskolin and related compounds
US20180020709A1 (en) * 2014-04-16 2018-01-25 Purecircle Usa Inc. Rebaudioside m biosynthetic production and recovery methods
EP3179869A1 (en) 2014-08-11 2017-06-21 Evolva SA Production of steviol glycosides in recombinant hosts
CN105850709B (zh) 2015-01-23 2018-08-21 谱赛科美国公司 甜叶菊新品种817096谱星5号及高rd含量甜菊糖苷的制备
CN107072237B (zh) 2014-09-02 2021-12-14 谱赛科有限责任公司 甜菊提取物
CN105850727B (zh) 2015-01-23 2018-12-07 谱赛科美国公司 甜叶菊新品种及高rd、rm含量甜菊糖苷的制备
US10612064B2 (en) 2014-09-09 2020-04-07 Evolva Sa Production of steviol glycosides in recombinant hosts
EP4166010A1 (en) * 2014-09-19 2023-04-19 PureCircle SDN BHD High-purity steviol glycosides
WO2016048994A2 (en) * 2014-09-23 2016-03-31 President And Fellows Of Harvard College Systems and methods of cell-free protein synthesis in droplets and other compartments
CN113016623A (zh) * 2014-09-26 2021-06-25 谱赛科美国股份有限公司 用于甜菊的单核苷酸多态性(snp)标志物
AU2015326892B2 (en) 2014-10-01 2022-03-10 Danstar Ferment Ag Methods and materials for biosynthesis of mogroside compounds
BR112017006139B1 (pt) * 2014-10-03 2022-04-05 Conagen Inc Método para a sintetização de rebaudiosídeo m ou rebaudiosídeo d e rebaudiosídeo m
WO2016055578A1 (en) * 2014-10-08 2016-04-14 Dsm Ip Assets B.V. Steviol glycoside production
WO2016073740A1 (en) * 2014-11-05 2016-05-12 Manus Biosynthesis, Inc. Microbial production of steviol glycosides
WO2016075302A1 (en) * 2014-11-13 2016-05-19 Evolva Sa Methods and materials for biosynthesis of manoyl oxide
CN108522291B (zh) 2015-01-23 2022-01-04 谱赛科美国公司 利用甜叶菊新品种814011谱星3号制备高rm含量甜菊糖苷的方法
CN108337892B (zh) 2015-01-30 2022-06-24 埃沃尔瓦公司 在重组宿主中生产甜菊醇糖苷
US10604743B2 (en) * 2015-03-16 2020-03-31 Dsm Ip Assets B.V. UDP-glycosyltransferases
US10294499B2 (en) 2015-05-28 2019-05-21 Evolva Sa Biosynthesis of phenylpropanoids and phenylpropanoid derivatives
BR112017025709B1 (pt) 2015-05-29 2024-03-05 Cargill, Incorporated Método para produzir glicosídeos de esteviol
EP4063513A1 (en) 2015-05-29 2022-09-28 Cargill, Incorporated Heat treatment to produce glycosides
CA2987585C (en) 2015-05-29 2023-08-29 Cargill, Incorporated Fermentation methods for producing steviol glycosides using high ph and compositions obtained therefrom
US11066688B2 (en) 2015-07-10 2021-07-20 Dsm Ip Assets B.V. Method for preparing a steviol glycoside composition
MY192148A (en) * 2015-07-10 2022-08-02 Dsm Ip Assets Bv Steviol glycoside composition
EP3331373A4 (en) 2015-08-06 2019-07-10 Cargill, Incorporated FERMENTATION PROCESS FOR THE PREPARATION OF STEVIOL GLYCOSIDES
CN108271391A (zh) * 2015-08-07 2018-07-10 埃沃尔瓦公司 重组宿主中的甜菊醇糖苷的产生
WO2017034942A1 (en) 2015-08-21 2017-03-02 Manus Biosynthesis, Inc. Increasing productivity of e. coli host cells that functionally express p450 enzymes
US9765117B2 (en) * 2015-08-24 2017-09-19 Romek Figa Peptides for treating cancer
WO2017060318A2 (en) 2015-10-05 2017-04-13 Dsm Ip Assets B.V. Kaurenoic acid hydroxylases
PL3367816T3 (pl) 2015-10-26 2024-05-06 Purecircle Usa Inc. Kompozycje glikozydów stewiolowych
WO2017093895A1 (en) 2015-11-30 2017-06-08 Purecircle Sdn Bhd Process for producing high purity steviol glycosides
EP3387136B1 (en) * 2015-12-10 2022-01-05 Evolva SA Production of steviol glycosides in recombinant hosts
CN108712864A (zh) 2015-12-15 2018-10-26 谱赛科美国股份有限公司 甜菊醇糖苷组合物
WO2017153538A1 (en) 2016-03-11 2017-09-14 Evolva Sa Production of steviol glycosides in recombinant hosts
WO2017178632A1 (en) 2016-04-13 2017-10-19 Evolva Sa Production of steviol glycosides in recombinant hosts
SG11201809483UA (en) * 2016-05-16 2018-11-29 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3458599A1 (en) 2016-05-16 2019-03-27 Evolva SA Production of steviol glycosides in recombinant hosts
CN109689878A (zh) * 2016-06-06 2019-04-26 泰特&莱尔组分美国公司 糖基化甜菊醇糖苷组合物和制备糖基化甜菊醇糖苷组合物的方法
AU2017284104B2 (en) 2016-06-15 2020-06-25 Codexis, Inc. Engineered beta-glucosidases and glucosylation methods
JP7404068B2 (ja) 2016-08-12 2023-12-25 アミリス， インコーポレイテッド レバウジオシドの高効率の産生のためのｕｄｐ依存性グリコシルトランスフェラーゼ
CA3041152A1 (en) 2016-10-21 2018-04-26 Pepsico, Inc. Method for preparing rebaudioside n using enzymatic method
CA3041150A1 (en) 2016-10-21 2018-04-26 Pepsico, Inc. Method for preparing rebaudioside c using enzymatic method
WO2018072211A1 (zh) 2016-10-21 2018-04-26 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙j的方法
WO2018083338A1 (en) 2016-11-07 2018-05-11 Evolva Sa Production of steviol glycosides in recombinant hosts
WO2018124141A1 (ja) * 2016-12-27 2018-07-05 サントリーホールディングス株式会社 ステビオール配糖体ヘキソース転移酵素およびそれをコードする遺伝子
EP3564251A4 (en) * 2016-12-27 2020-12-23 Suntory Holdings Limited NEW STEVIOL GLYCOSIDE, ITS PRODUCTION PROCESS AND COMPOSITION OF SWEETENER CONTAINER
CN108341781B (zh) * 2017-01-25 2021-09-03 中国科学院分子植物科学卓越创新中心 植物次生代谢产物生物合成途径中相关酶类的解析方法
KR102656420B1 (ko) 2017-01-26 2024-04-09 마누스 바이오, 인크. 터페노이드 생성물의 미생물 생산을 위한 대사 조작
TW202237833A (zh) 2017-02-03 2022-10-01 美商克迪科思股份有限公司 經工程處理之醣基轉移酶及甜菊糖之葡萄糖苷化方法
MY193581A (en) * 2017-02-21 2022-10-19 Univ Duke Compositions and methods for robust dynamic metabolic control
SG10202111903TA (en) * 2017-03-31 2021-12-30 Suntory Holdings Ltd Novel steviol glycoside and production method therefor, and sweetener composition containing same
JP7011649B2 (ja) * 2017-04-12 2022-02-10 サントリーホールディングス株式会社 ステビア由来ラムノース合成酵素及び遺伝子
EP3624599A4 (en) * 2017-05-15 2020-11-18 PureCircle USA Inc. HIGHLY PURE STEVIOL GLYCOSIDE
WO2018211032A1 (en) 2017-05-17 2018-11-22 Evolva Sa Production of steviol glycosides in recombinant hosts
US11248248B2 (en) 2017-06-15 2022-02-15 Evolva Sa Production of mogroside compounds in recombinant hosts
WO2019033064A1 (en) * 2017-08-11 2019-02-14 Amyris, Inc. KAURENE OXIDASE FROM PISUM SATIVUM FOR HIGH-PERFORMANCE PRODUCTION OF REBAUDIOSIDES
WO2019055325A2 (en) * 2017-09-12 2019-03-21 Biocapital Holdings, Llc BIOOGICAL DEVICES AND METHODS OF USING THE SAME TO PRODUCE STEVIOL GLYCOSIDES
WO2019110684A1 (en) 2017-12-05 2019-06-13 Evolva Sa Production of steviol glycosides in recombinant hosts
US11541105B2 (en) 2018-06-01 2023-01-03 The Research Foundation For The State University Of New York Compositions and methods for disrupting biofilm formation and maintenance
MX2021000605A (es) 2018-07-16 2021-06-23 Manus Bio Inc Producción de glucósidos de esteviol a través de biotransformación de células enteras.
WO2020028039A1 (en) 2018-07-30 2020-02-06 Codexis, Inc. Engineered glycosyltransferases and steviol glycoside glucosylation methods
CN113302298B (zh) 2018-11-09 2025-02-25 银杏生物制品公司 罗汉果甙的生物合成
EP3883367A4 (en) 2018-11-20 2022-10-05 PureCircle USA Inc. ANEUPLOID STEVIA VARIETY AP-1
KR102709594B1 (ko) * 2019-06-25 2024-09-26 마누스 바이오, 인크. 우리딘 다이포스페이트-의존성 글라이코실트랜스퍼라제 효소
KR20220044789A (ko) 2019-08-08 2022-04-11 도아고세이가부시키가이샤 물이해체성 접착제 조성물
CN110592040A (zh) * 2019-09-18 2019-12-20 江苏施宇甜生物科技有限公司 一种用于生产upd糖基转移酶的重组枯草芽孢杆菌的工艺
CN111424065B (zh) * 2020-03-24 2022-08-16 金禾益康(北京)生物科技有限公司 使用糖基转移酶对甜菊糖苷类化合物进行糖基化方法
WO2023006179A1 (en) 2020-07-27 2023-02-02 Dsm Ip Assets B.V. Method to produce retinyl acetate
CN112147253B (zh) * 2020-09-25 2022-04-01 安徽瑞思威尔科技有限公司 一种灵芝酒中42种有效成分的UPC2-PDA-Q-Tof/MS检测方法
WO2022084482A1 (en) 2020-10-22 2022-04-28 Dsm Ip Assets B.V. Microorganisms for diterpene production
US20250320470A1 (en) * 2020-12-23 2025-10-16 Samyang Corporation Glycosyltransferase and steviol glucoside preparation method using same
CN112877355A (zh) * 2021-01-22 2021-06-01 杜云龙 一种利用烟草表达三七皂苷的方法
WO2022245988A2 (en) * 2021-05-18 2022-11-24 Biokai, Inc. Production of carotenoids
CN115478060B (zh) * 2021-06-16 2023-11-28 弈柯莱生物科技(集团)股份有限公司 一种糖基转移酶及其应用
WO2023006851A1 (en) 2021-07-27 2023-02-02 Dsm Ip Assets B.V. Fermentative production of retinyl acetate in the presence of ethanol
CN115975827A (zh) * 2021-10-15 2023-04-18 中国科学院分子植物科学卓越创新中心 用于甜茶素生产的宿主细胞及方法
WO2023121426A1 (ko) * 2021-12-24 2023-06-29 주식회사 삼양사 레바우디오사이드의 생산
CN114703217B (zh) * 2022-03-26 2025-09-02 江苏省中国科学院植物研究所 一种甜菊自交系种子转基因体系的建立方法
CN114752575B (zh) * 2022-04-07 2023-06-13 内蒙古工业大学 一种nad+依赖性脱氢酶基因及其在提高辅酶q10产量中的应用
WO2024010074A1 (ja) 2022-07-07 2024-01-11 サントリーホールディングス株式会社 ステビオール配糖体生合成変異酵素とその利用
CN116162607B (zh) * 2023-01-04 2025-07-01 中国科学院大连化学物理研究所 一种甜菊糖苷糖基转移酶基因及其编码产物与应用
CN116004563B (zh) * 2023-01-17 2023-11-17 华中农业大学 一种茶树类黄酮糖基转移酶及其应用
WO2024197447A1 (zh) * 2023-03-24 2024-10-03 森瑞斯生物科技(深圳)有限公司 HMG-CoA还原酶突变体及其在生产萜类化合物中的应用
WO2024228615A1 (en) * 2023-05-03 2024-11-07 Purecircle Sdn. Bhd. High-purity steviol glycosides

Family Cites Families (153)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58149697A (ja) 1982-02-27 1983-09-06 Dainippon Ink & Chem Inc β−１，３グリコシルステビオシドの製造方法
JPS59101408A (ja) * 1982-12-02 1984-06-12 Junichi Iwamura 植物生長調整剤
US5198360A (en) 1990-01-19 1993-03-30 Eli Lilly And Company Dna sequence conferring a plaque inhibition phenotype
US6946587B1 (en) 1990-01-22 2005-09-20 Dekalb Genetics Corporation Method for preparing fertile transgenic corn plants
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
JPH03277275A (ja) 1990-03-28 1991-12-09 Dainippon Ink & Chem Inc 新規酵素及びその酵素を用いた配糖体の製造方法
US5204253A (en) 1990-05-29 1993-04-20 E. I. Du Pont De Nemours And Company Method and apparatus for introducing biological substances into living cells
US5306862A (en) 1990-10-12 1994-04-26 Amoco Corporation Method and composition for increasing sterol accumulation in higher plants
US5460949A (en) 1990-11-15 1995-10-24 Amoco Corporation Method and composition for increasing the accumulation of squalene and specific sterols in yeast
US5712112A (en) 1992-11-04 1998-01-27 National Science Council Of R.O.C. Gene expression system comprising the promoter region of the alpha-amylase genes
AU691510B2 (en) 1993-07-15 1998-05-21 Neose Pharmaceuticals Method of synthesizing saccharide compositions
US7186891B1 (en) 1996-04-12 2007-03-06 University Of Kentucky, Research Foundation Plant cells and plants expressing chimeric isoprenoid synthases
JPH10117776A (ja) 1996-10-22 1998-05-12 Japan Tobacco Inc インディカイネの形質転換方法
EP1021551A1 (en) 1997-10-06 2000-07-26 Centre National De La Recherche Scientifique Plant fatty acid hydroxylase genes
US6255557B1 (en) 1998-03-31 2001-07-03 Her Majesty The Queen In Right Of Canada As Represented By The Ministerof Agriculture And Agri-Food Canada Stevia rebaudiana with altered steviol glycoside composition
AU3169999A (en) 1998-04-14 1999-11-01 Kyowa Hakko Kogyo Co. Ltd. Process for producing isoprenoid compounds by using microorganisms and method for detecting compounds having antibacterial or herbicidal activity
TWI250210B (en) 1998-05-06 2006-03-01 Dsm Ip Assets Bv An isolated DNA sequence coding for an enzyme involved in the mevalonate pathway or the pathway from isopentenyl pyrophosphate to farnesyl pyrophosphate
EP0955363A3 (en) 1998-05-06 2004-01-28 F. Hoffmann-La Roche Ag Dna sequences encoding enzymes involved in production of isoprenoids
EP2305825B1 (en) 1998-07-06 2015-01-14 DCV Inc. doing business as Bio-Technical Resourses Method of vitamin production
JP4579415B2 (ja) 1998-07-06 2010-11-10 ディーシーブイ・インコーポレイテッド・ドゥーイング・ビジネス・アズ・バイオ−テクニカル・リソーシィズ ビタミン製造方法
AR021636A1 (es) 1998-12-17 2002-07-31 Rubicon Forests Holdings Ltd Materiales y metodos para la modificacion del contenido, la composicion y el metabolismo de los isoprenoides
WO2000037663A2 (en) 1998-12-23 2000-06-29 The Samuel Roberts Noble Foundation, Inc. Plant transformation process
CA2369844C (en) 1999-04-15 2013-02-26 Calgene Llc Nucleic acid sequences to proteins involved in tocopherol synthesis
WO2000063400A2 (en) 1999-04-21 2000-10-26 The Samuel Roberts Noble Foundation Plant transformation process
AU6280300A (en) 1999-08-04 2001-03-05 Adelbert Bacher Isoprenoid biosynthesis
WO2001012828A1 (en) 1999-08-18 2001-02-22 Paradigm Genetics, Inc. Methods and apparatus for transformation of monocotyledenous plants using agrobacterium in combination with vacuum filtration
AU2001269685A1 (en) 2000-05-03 2001-11-12 The Salk Institute For Biological Studies Crystallization of 4-diphosphocytidyl-2-c-methylerythritol synthesis
DE10027821A1 (de) 2000-06-05 2001-12-06 Adelbert Bacher Der Mevalonat-unabhängige Isoprenoidbiosyntheseweg
EP2292776A1 (en) 2000-07-31 2011-03-09 Danisco US Inc. Manipulation of genes of the mevalonate and isoprenoid pathways to create novel traits in transgenic organisms
WO2002016655A2 (en) 2000-08-24 2002-02-28 The Scripps Research Institute Stress-regulated genes of plants, transgenic plants containing same, and methods of use
US6689601B2 (en) 2000-09-01 2004-02-10 E. I. Du Pont De Nemours And Company High growth methanotropic bacterial strain
US6818424B2 (en) 2000-09-01 2004-11-16 E. I. Du Pont De Nemours And Company Production of cyclic terpenoids
US6660507B2 (en) 2000-09-01 2003-12-09 E. I. Du Pont De Nemours And Company Genes involved in isoprenoid compound production
EP1409530A2 (en) 2000-09-19 2004-04-21 Microbia, Inc. Modulation of secondary metabolite production by zinc binuclear cluster proteins
US20030219798A1 (en) 2000-09-29 2003-11-27 Gokarn Ravi R. Isoprenoid production
CN1345963A (zh) * 2000-09-29 2002-04-24 上海博德基因开发有限公司 一种新的多肽——udp糖基转移酶(ugt)及钴胺素结合蛋白9.57和编码这种多肽的多核苷酸
US6949362B2 (en) 2000-12-12 2005-09-27 E. I. Du Pont De Nemours And Company Rhodococcus cloning and expression vectors
US7838287B2 (en) 2001-01-25 2010-11-23 Evolva Sa Library of a collection of cells
US20040078846A1 (en) 2002-01-25 2004-04-22 Desouza Mervyn L. Carotenoid biosynthesis
US20050003474A1 (en) 2001-01-26 2005-01-06 Desouza Mervyn L. Carotenoid biosynthesis
DE10201458A1 (de) 2001-04-11 2002-10-17 Adelbert Bacher Intermediate und Enzyme des Mevalonat-unabhängigen Isoprenoidbiosyntheseweg
US7034140B2 (en) 2001-04-24 2006-04-25 E.I. Du Pont De Nemours And Company Genes involved in isoprenoid compound production
ATE405638T1 (de) 2001-06-06 2008-09-15 Dsm Ip Assets Bv Verbesserte isoprenoid herstellung
WO2003008540A2 (en) 2001-06-22 2003-01-30 Syngenta Participations Ag Abiotic stress responsive polynucleotides and polypeptides
NZ513755A (en) 2001-08-24 2001-09-28 Ann Rachel Holmes Protein expression system in yeast comprising a vector encoding a heterologous membrane protein and its application in screening for drugs
US20040072311A1 (en) 2001-08-28 2004-04-15 Dicosimo Deana J. Production of cyclic terpenoids
US20040010815A1 (en) 2001-09-26 2004-01-15 Lange B. Markus Identification and characterization of plant genes
US7172886B2 (en) 2001-12-06 2007-02-06 The Regents Of The University Of California Biosynthesis of isopentenyl pyrophosphate
JP2005185101A (ja) 2002-05-30 2005-07-14 National Institute Of Agrobiological Sciences 植物の全長ｃＤＮＡおよびその利用
DE60329505D1 (de) 2002-08-20 2009-11-12 Suntory Holdings Ltd Neue glycosyltransferase-gene
DE60320812D1 (en) 2002-09-27 2008-06-19 Dsm Ip Assets Bv Squalene synthase (sqs) gen
US7098000B2 (en) 2003-06-04 2006-08-29 E. I. Du Pont De Nemoure And Company Method for production of C30-aldehyde carotenoids
ATE426662T1 (de) 2003-06-12 2009-04-15 Dsm Ip Assets Bv Feedback resistante mevalonate kinase
US7569389B2 (en) 2004-09-30 2009-08-04 Ceres, Inc. Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics
CN101023181A (zh) 2004-05-21 2007-08-22 加利福尼亚大学董事会 提高类异戊二烯化合物的产生的方法
WO2006069610A2 (en) 2004-07-02 2006-07-06 Metanomics Gmbh Process for the production of fine chemicals
US20060014264A1 (en) 2004-07-13 2006-01-19 Stowers Institute For Medical Research Cre/lox system with lox sites having an extended spacer region
JPWO2006016395A1 (ja) * 2004-08-09 2008-05-01 国立大学法人東北大学 Ｕｄｐ−グルクロニル基転移酵素およびその遺伝子
US7572609B2 (en) 2004-08-19 2009-08-11 Dsm Ip Assets B.V. Production of isoprenoids
US7923552B2 (en) 2004-10-18 2011-04-12 SGF Holdings, LLC High yield method of producing pure rebaudioside A
JP4841562B2 (ja) 2004-12-14 2011-12-21 ディーエスエム アイピー アセッツ ビー．ブイ． 改良型メバロネートキナーゼ
WO2006092449A2 (en) 2005-03-02 2006-09-08 Metanomics Gmbh Process for the production of fine chemicals
WO2006096392A2 (en) 2005-03-04 2006-09-14 Diversa Corporation Enzymes involved in astaxanthin, carotenoid and isoprenoid biosynthetic pathways, genes encoding them and methods of making and using them
WO2006093289A1 (ja) * 2005-03-04 2006-09-08 National Institute Of Advanced Industrial Science And Technology Udp-キシロースの製造方法
US7989677B2 (en) 2005-08-17 2011-08-02 Nestec S. A. Nucleic acids and proteins associated with sucrose accumulation in coffee
EP1916888A4 (en) 2005-08-22 2011-05-18 Co2 Boost Llc DEVICE AND PROCESS FOR GENERATING CO2 USED FOR HARVEST PRODUCTION AND SUBMARINE GARDENING
US8293307B2 (en) * 2005-10-11 2012-10-23 Purecircle Sdn Bhd Process for manufacturing a sweetener and use thereof
DK2526778T3 (en) 2005-11-23 2017-03-27 Coca Cola Co Natural High Strength Sweetener Compositions with Improved Time Profile and / or Taste Profile, Methods of Formulation thereof and Applications thereof
US20070116833A1 (en) * 2005-11-23 2007-05-24 The Coca-Cola Company High-Potency Sweetener Composition with Calcium and Compositions Sweetened Therewith
KR100784261B1 (ko) 2006-01-02 2007-12-11 한국과학기술원 탄저균의 포자외막 단백질을 이용한 목적단백질의 미생물표면발현방법
US7927851B2 (en) 2006-03-21 2011-04-19 Vineland Research And Innovation Centre Compositions having ent-kaurenoic acid 13-hydroxylase activity and methods for producing same
WO2008034648A1 (en) * 2006-04-05 2008-03-27 Metanomics Gmbh Process for the production of a fine chemical
US8114645B2 (en) 2006-05-19 2012-02-14 The Regents Of The University Of California Methods for increasing isoprenoid and isoprenoid precursor production by modulating fatty acid levels
EP2035827B1 (en) 2006-06-19 2012-10-17 Givaudan S.A. Nucleic acid, polypeptide and its use
WO2008008256A2 (en) 2006-07-07 2008-01-17 The Regents Of The University Of California Methods for enhancing production of isoprenoid compounds by host cells
DK3020799T3 (da) 2006-09-26 2020-09-21 Amyris Biotechnologies Inc Fremstilling af isoprenoider og isoprenoid-prækursorer
US7741119B2 (en) 2006-09-28 2010-06-22 E. I. Du Pont De Nemours And Company Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production
US7629156B2 (en) 2006-09-28 2009-12-08 E.I. Du Pont De Nemours And Company Ethanol production in fermentation of mixed sugars containing xylose
CN101200480B (zh) 2006-12-15 2011-03-30 成都华高药业有限公司 莱鲍迪甙a的提取方法
JP4915917B2 (ja) 2006-12-22 2012-04-11 独立行政法人農業・食品産業技術総合研究機構 ラクト−ｎ−ビオースｉ及びガラクト−ｎ−ビオースの製造方法
MY148593A (en) 2007-01-22 2013-05-15 Cargill Inc Method of producing purified rebaudioside a compositions using solvent/antisolvent crystallization
US20130071521A1 (en) * 2007-03-14 2013-03-21 Pepsico, Inc. Rebaudioside d sweeteners and food products sweetened with rebaudioside d
JP2008237110A (ja) 2007-03-27 2008-10-09 Institute Of Physical & Chemical Research ステビオール合成酵素遺伝子及びステビオールの製造方法
WO2008144060A2 (en) 2007-05-17 2008-11-27 Tetravitae Bioscience, Inc. Methods and compositions for producing solvents
US20080292775A1 (en) 2007-05-22 2008-11-27 The Coca-Cola Company Delivery Systems for Natural High-Potency Sweetener Compositions, Methods for Their Formulation, and Uses
WO2009005704A1 (en) 2007-07-03 2009-01-08 The Regents Of The University Of California Methods of increasing isoprenoid or isoprenoid precursor production
JP2009034080A (ja) 2007-08-03 2009-02-19 Sanei Gen Ffi Inc 新規糖転移酵素、及びそれを利用した配糖体の製造
US7964232B2 (en) 2007-09-17 2011-06-21 Pepsico, Inc. Steviol glycoside isomers
US8809059B2 (en) 2007-09-21 2014-08-19 Basf Plant Science Gmbh Plants with increased yield
NZ585720A (en) 2007-12-03 2012-05-25 Dsm Ip Assets Bv Novel nutraceutical compositions containing stevia extract or stevia extract constituents and uses thereof
CA2710629C (en) 2007-12-27 2016-06-21 Mcneil Nutritionals, Llc Synergistic sweetening compositions
US20110061124A1 (en) * 2008-02-20 2011-03-10 Ceres, Inc Nucleotide sequences and corresponding polypeptides conferring improved nitrogen use efficiency characteristics in plants
TWI475963B (zh) * 2008-02-25 2015-03-11 Coca Cola Co 甜菊糖苷ａ衍生性產物以及製造彼的方法
CA2728285A1 (en) 2008-03-03 2009-09-11 Joule Unlimited, Inc. Engineered co2 fixing microorganisms producing carbon-based products of interest
WO2009140394A1 (en) 2008-05-13 2009-11-19 Cargill, Incorporated Separation of rebaudioside a from stevia glycosides using chromatography
CN101314776B (zh) 2008-07-11 2010-11-03 南京农业大学 二磷酸尿核甘葡萄糖基转移酶基因及其所编码的蛋白质
WO2010021001A2 (en) 2008-08-19 2010-02-25 Kaushik Ramakrishnan S Process for preparing sweetener from stevia rebaudiana
KR102061165B1 (ko) 2008-10-03 2019-12-31 모리타 가가쿠 고교 가부시키가이샤 신규 스테비올 배당체
CN101720910B (zh) 2008-10-23 2012-07-25 大闽食品（漳州）有限公司 一种甜菊糖甙的制备方法
US8614085B2 (en) 2009-02-27 2013-12-24 Butamax(Tm) Advanced Biofuels Llc Yeast with increased butanol tolerance involving a multidrug efflux pump gene
US20100297722A1 (en) 2009-05-20 2010-11-25 Board Of Trustees Of Southern Illinois University Transgenic moss producing terpenoids
ES2660698T3 (es) 2009-06-08 2018-03-23 Jennewein Biotechnologie Gmbh Síntesis de HMO
KR20120027363A (ko) 2009-06-16 2012-03-21 이피씨 (베이징) 내추럴 프로덕츠 컴퍼니, 리미티드 뒷맛을 감소시키거나 제거하기 위한 레바우디오사이드 ｄ를 포함하는 조성물 및 그의 제조 방법
US20120164083A1 (en) 2009-09-04 2012-06-28 Redpoint Bio Corporation Sweetness Enhancers Including Rebaudioside A or D
US20120230922A1 (en) 2009-09-22 2012-09-13 Redpoint Bio Corporation Novel Polymorphs of Rebaudioside C and Methods for Making and Using the Same
ES2661539T3 (es) 2009-10-15 2018-04-02 Purecircle Sdn Bhd Rebaudiósido D de pureza elevada y aplicaciones
US8299224B2 (en) 2009-10-15 2012-10-30 Purecircle Sdn Bhd High-purity Rebaudioside D
US8703224B2 (en) 2009-11-04 2014-04-22 Pepsico, Inc. Method to improve water solubility of Rebaudioside D
EP2594648B1 (en) 2009-11-10 2016-03-16 Massachusetts Institute of Technology Microbial engineering for the production of chemical and pharmaceutical products from the isoprenoid pathway
WO2011062748A1 (en) 2009-11-23 2011-05-26 E.I. Du Pont De Nemours And Company Sucrose transporter genes for increasing plant seed lipids
CN106834320B (zh) 2009-12-10 2021-05-25 明尼苏达大学董事会 Tal效应子介导的dna修饰
WO2011090709A1 (en) 2009-12-28 2011-07-28 The Coca-Cola Company Sweetness enhancers, compositions thereof, and methods for use
KR101244315B1 (ko) 2010-10-19 2013-03-14 이화여자대학교 산학협력단 에탄올―저항성 효모 유전자 및 이의 용도
US9441233B2 (en) 2010-05-06 2016-09-13 Ceres, Inc. Transgenic plants having increased biomass
EP2571991A1 (en) 2010-05-20 2013-03-27 Evolva SA Method of producing isoprenoid compounds in yeast
US9249420B2 (en) 2010-05-31 2016-02-02 Vib Vzw Isobutanol production using yeasts with modified transporter expression
CN105671108A (zh) 2010-06-02 2016-06-15 沃维公司 甜菊糖苷的重组生产
US20120021111A1 (en) 2010-07-23 2012-01-26 Aexelon Therapeutics, Inc. Natural Low Caloric Sweetener Compositions for Use in Beverages, Foods and Pharmaceuticals, and Their Methods of Manufacture
US20120083593A1 (en) 2010-10-01 2012-04-05 Shanghai Yongyou Bioscience Inc. Separation and Purification of Stevioside and Rebaudioside A
WO2012075030A1 (en) * 2010-11-30 2012-06-07 Massachusetts Institute Of Technology Microbial production of natural sweeteners, diterpenoid steviol glycosides
KR20130014227A (ko) 2011-07-29 2013-02-07 한국생명공학연구원 신규한 α-글루코실 스테비오사이드 및 이의 제조 방법
WO2013022989A2 (en) 2011-08-08 2013-02-14 Evolva Sa Recombinant production of steviol glycosides
KR101815063B1 (ko) 2011-08-08 2018-01-05 에볼바 에스아 사프란 화합물의 재조합 생성을 위한 방법 및 물질
WO2013076577A1 (en) 2011-11-23 2013-05-30 Evolva Sa Methods and materials for enzymatic synthesis of mogroside compounds
CN103159808B (zh) 2011-12-09 2017-03-29 上海泓博智源医药股份有限公司 一种制备天然甜味剂的工艺方法
ES2787899T3 (es) 2011-12-19 2020-10-19 Purecircle Sdn Bhd Métodos para purificar glucósidos de esteviol
CN104203005A (zh) 2012-01-23 2014-12-10 帝斯曼知识产权资产管理有限公司 二萜的生产
CN102559528B (zh) 2012-02-09 2013-08-21 南京工业大学 一种产甜叶菊糖基转移酶ugt76g1的基因工程菌及其应用
CA2867112C (en) 2012-03-16 2021-04-20 Suntory Holdings Limited Steviol glucosyltransferases and genes encoding the same
CN103974628B (zh) 2012-05-22 2019-04-05 谱赛科有限责任公司 高纯度的甜菊醇糖苷
EP2902410B1 (en) 2012-09-29 2022-01-05 Sichuan Ingia Biosynthetic Co., Ltd. Method for producing stevioside compounds by microorganism
US20150342234A1 (en) 2012-12-05 2015-12-03 Evolva Sa Steviol Glycoside Compositions Sensory Properties
SG11201505597SA (en) 2013-02-06 2015-08-28 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
EP2953809B1 (en) 2013-02-11 2016-12-28 Dana Belgium N.V. Hydrostatic and direct drive transmission
EP2954061B1 (en) 2013-02-11 2023-11-22 Evolva SA Efficient production of steviol glycosides in recombinant hosts
MX367033B (es) 2013-05-31 2019-08-02 Dsm Ip Assets Bv Microorganismos para la produccion de diterpeno.
BR112015029601A2 (pt) 2013-05-31 2017-09-26 Dsm Ip Assets Bv produção de diterpeno extracelular
KR101559478B1 (ko) 2013-06-24 2015-10-13 한국생명공학연구원 효소전환법을 이용한 천연 고감미료의 제조방법
CN108064135A (zh) 2013-07-15 2018-05-22 帝斯曼知识产权资产管理有限公司 二萜的生产
US10273519B2 (en) 2013-07-23 2019-04-30 Dsm Ip Assets B.V. Diterpene production in Yarrowia
CA2917615A1 (en) 2013-07-31 2015-02-05 Dsm Ip Assets B.V. Steviol glycosides
WO2015014959A1 (en) 2013-07-31 2015-02-05 Dsm Ip Assets B.V. Recovery of steviol glycosides
WO2015016393A1 (ja) 2013-08-02 2015-02-05 サントリーホールディングス株式会社 ヘキセノール配糖体化酵素の利用方法
CN103397064B (zh) 2013-08-14 2015-04-15 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙m的方法
US20160215306A1 (en) 2013-08-30 2016-07-28 Evolva Sa Method for producing modified resveratrol
WO2015051454A1 (en) 2013-10-07 2015-04-16 Vineland Research And Innovation Centre Compositions and methods for producing steviol and steviol glycosides
SG10201807693YA (en) 2014-03-07 2018-10-30 Evolva Sa Methods for recombinant production of saffron compounds
EP3179869A1 (en) 2014-08-11 2017-06-21 Evolva SA Production of steviol glycosides in recombinant hosts
US10612064B2 (en) 2014-09-09 2020-04-07 Evolva Sa Production of steviol glycosides in recombinant hosts
CN108337892B (zh) 2015-01-30 2022-06-24 埃沃尔瓦公司 在重组宿主中生产甜菊醇糖苷
US10604743B2 (en) 2015-03-16 2020-03-31 Dsm Ip Assets B.V. UDP-glycosyltransferases
CN104845990A (zh) 2015-06-11 2015-08-19 山东大学 拟南芥糖基转移酶基因ugt73c7在提高植物抗病性中的应用
CN108271391A (zh) 2015-08-07 2018-07-10 埃沃尔瓦公司 重组宿主中的甜菊醇糖苷的产生

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