SG10201709458QA - Recombinant production of steviol glycosides - Google Patents

Recombinant production of steviol glycosides

Info

Publication number
SG10201709458QA
SG10201709458QA SG10201709458QA SG10201709458QA SG10201709458QA SG 10201709458Q A SG10201709458Q A SG 10201709458QA SG 10201709458Q A SG10201709458Q A SG 10201709458QA SG 10201709458Q A SG10201709458Q A SG 10201709458QA SG 10201709458Q A SG10201709458Q A SG 10201709458QA
Authority
SG
Singapore
Prior art keywords
recombinant production
steviol glycosides
steviol
glycosides
recombinant
Prior art date
Application number
SG10201709458QA
Inventor
Ganesh M Kishore
Michael Motion
Paula M Hicks
Jorgen Hansen
Jens Houghton-Larsen
Esben Halkjaer Hansen
Michael Dalgaard Mikkelsen
Sabina Tavares
Charlotte Blom
Original Assignee
Evolva Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva Inc filed Critical Evolva Inc
Publication of SG10201709458QA publication Critical patent/SG10201709458QA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/44Preparation of O-glycosides, e.g. glucosides
    • C12P19/56Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • A23L2/60Sweeteners
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30Artificial sweetening agents
    • A23L27/33Artificial sweetening agents containing sugars or derivatives
    • A23L27/36Terpene glycosides
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/20Carbocyclic rings
    • C07H15/24Condensed ring systems having three or more rings
    • C07H15/256Polyterpene radicals
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0006Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0012Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042NADPH-cytochrome P450 reductase (1.6.2.4)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1077Pentosyltransferases (2.4.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1085Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90Isomerases (5.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P15/00Preparation of compounds containing at least three condensed carbocyclic rings
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/40Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42Hydroxy-carboxylic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/40Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/58Aldonic, ketoaldonic or saccharic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y101/00Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01088Hydroxymethylglutaryl-CoA reductase (1.1.1.88)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y106/00Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/02Oxidoreductases acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12Y106/02004NADPH-hemoprotein reductase (1.6.2.4), i.e. NADP-cytochrome P450-reductase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y114/00Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C12Y114/13078Ent-kaurene oxidase (1.14.13.78)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y114/00Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C12Y114/13079Ent-kaurenoic acid oxidase (1.14.13.79)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y204/00Glycosyltransferases (2.4)
    • C12Y204/01Hexosyltransferases (2.4.1)
    • C12Y204/01126Hydroxycinnamate 4-beta-glucosyltransferase (2.4.1.126)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y205/00Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01029Geranylgeranyl diphosphate synthase (2.5.1.29)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y402/00Carbon-oxygen lyases (4.2)
    • C12Y402/03Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03019Ent-kaurene synthase (4.2.3.19)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y505/00Intramolecular lyases (5.5)
    • C12Y505/01Intramolecular lyases (5.5.1)
    • C12Y505/01012Copalyl diphosphate synthase (5.5.1.12)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of bulk chemicals
    • Y02P20/52Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Physiology (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
SG10201709458QA 2010-06-02 2011-06-02 Recombinant production of steviol glycosides SG10201709458QA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US35055310P 2010-06-02 2010-06-02
US201161434582P 2011-01-20 2011-01-20
US201161471622P 2011-04-04 2011-04-04

Publications (1)

Publication Number Publication Date
SG10201709458QA true SG10201709458QA (en) 2017-12-28

Family

ID=45067080

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201709458QA SG10201709458QA (en) 2010-06-02 2011-06-02 Recombinant production of steviol glycosides
SG2012088548A SG186127A1 (en) 2010-06-02 2011-06-02 Recombinant production of steviol glycosides

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG2012088548A SG186127A1 (en) 2010-06-02 2011-06-02 Recombinant production of steviol glycosides

Country Status (14)

Country Link
US (5) US9562251B2 (en)
EP (2) EP3593633A1 (en)
JP (6) JP6177127B2 (en)
KR (3) KR101802547B1 (en)
CN (2) CN105671108A (en)
AU (1) AU2011261394C1 (en)
BR (5) BR112012030836B8 (en)
CA (2) CA2802627C (en)
CL (1) CL2012003373A1 (en)
MX (2) MX349121B (en)
MY (3) MY185668A (en)
NZ (3) NZ604915A (en)
SG (2) SG10201709458QA (en)
WO (1) WO2011153378A1 (en)

Families Citing this family (133)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9386797B2 (en) 2011-02-17 2016-07-12 Purecircle Sdn Bhd Glucosyl stevia composition
US8318459B2 (en) 2011-02-17 2012-11-27 Purecircle Usa Glucosyl stevia composition
US9392799B2 (en) 2011-02-17 2016-07-19 Purecircle Sdn Bhd Glucosyl stevia composition
US8790730B2 (en) 2005-10-11 2014-07-29 Purecircle Usa Process for manufacturing a sweetener and use thereof
US9107436B2 (en) 2011-02-17 2015-08-18 Purecircle Sdn Bhd Glucosylated steviol glycoside as a flavor modifier
US8257948B1 (en) 2011-02-17 2012-09-04 Purecircle Usa Method of preparing alpha-glucosyl Stevia composition
BR112012011073B8 (en) 2009-11-10 2023-01-10 Massachusetts Inst Technology METHOD FOR INCREASE TERPENOID PRODUCTION IN A CELL PRODUCING ONE OR MORE TERPENOIDS
US8927241B2 (en) 2009-11-10 2015-01-06 Massachusetts Institute Of Technology Microbial engineering for the production of chemical and pharmaceutical products from the isoprenoid pathway
EP3461342A1 (en) 2009-11-12 2019-04-03 PureCircle USA Inc. Granulation of a stevia sweetener
US10696706B2 (en) 2010-03-12 2020-06-30 Purecircle Usa Inc. Methods of preparing steviol glycosides and uses of the same
EP4108668A1 (en) 2010-03-12 2022-12-28 PureCircle USA Inc. High-purity steviol glycosides
EP2571991A1 (en) * 2010-05-20 2013-03-27 Evolva SA Method of producing isoprenoid compounds in yeast
CN105671108A (en) 2010-06-02 2016-06-15 沃维公司 Recombinant production of steviol glycosides
CA2819253A1 (en) * 2010-11-30 2012-06-07 Massachusetts Institute Of Technology Microbial production of natural sweeteners, diterpenoid steviol glycosides
US9029426B2 (en) 2010-12-13 2015-05-12 Purecircle Sdn Bhd Highly soluble Rebaudioside D
US9510611B2 (en) 2010-12-13 2016-12-06 Purecircle Sdn Bhd Stevia composition to improve sweetness and flavor profile
MX362676B (en) 2011-02-10 2019-01-31 Purecircle Usa Stevia composition.
US11690391B2 (en) 2011-02-17 2023-07-04 Purecircle Sdn Bhd Glucosylated steviol glycoside as a flavor modifier
US9603373B2 (en) 2011-02-17 2017-03-28 Purecircle Sdn Bhd Glucosyl stevia composition
US9474296B2 (en) 2011-02-17 2016-10-25 Purecircle Sdn Bhd Glucosyl stevia composition
US9894922B2 (en) 2011-05-18 2018-02-20 Purecircle Sdn Bhd Glucosyl rebaudioside C
ES2727031T3 (en) 2011-05-31 2019-10-11 Purecircle Usa Inc Stevia Composition
EP2713762B1 (en) 2011-06-03 2016-03-30 Purecircle USA Stevia composition
MX364306B (en) 2011-06-20 2019-04-22 Purecircle Usa Inc Stevia composition.
US9771434B2 (en) 2011-06-23 2017-09-26 Purecircle Sdn Bhd Products from stevia rebaudiana
EP3009508B1 (en) 2011-08-08 2020-11-25 Evolva SA Recombinant production of steviol glycosides
US10480019B2 (en) 2011-08-10 2019-11-19 Purecircle Sdn Bhd Process for producing high-purity rubusoside
MX2014002462A (en) 2011-09-07 2014-05-07 Purecircle Usa Inc Highly soluble stevia sweetener.
CN103031283B (en) * 2011-10-08 2015-07-08 成都华高瑞甜科技有限公司 Stevia rebaudiana enzyme VI and method for converting rebaudioside-A into rebaudioside-D
AU2012342114B2 (en) 2011-11-23 2017-12-21 Evolva Sa Methods and materials for enzymatic synthesis of mogroside compounds
ES2971273T3 (en) 2011-12-19 2024-06-04 Coca Cola Co Beverage comprising rebaudioside
FR2984743B1 (en) * 2011-12-23 2014-08-22 Oreal COSMETIC USE OF STEVIOL, STEVIOL GLYCOSIDE DERIVATIVE, OR ONE OF THEIR ISOMERS FOR STIMULATING, RESTORING OR REGULATING METABOLISM OF SKIN CELLS AND SEMI-MUCOUS MEMBRANES
CN104203005A (en) * 2012-01-23 2014-12-10 帝斯曼知识产权资产管理有限公司 Diterpene production
JP6251669B2 (en) * 2012-03-16 2017-12-20 サントリーホールディングス株式会社 Steviol glycoside enzyme and gene encoding the same
EP2832858B1 (en) * 2012-03-27 2020-07-08 Suntory Holdings Limited Method for producing steviol glycoside
US20130338348A1 (en) * 2012-03-30 2013-12-19 J.R. Simplot Company Steviol and steviol glycoside formation in plants
US9752174B2 (en) * 2013-05-28 2017-09-05 Purecircle Sdn Bhd High-purity steviol glycosides
CN110358795A (en) * 2012-05-22 2019-10-22 谱赛科有限责任公司 The steviol glycoside of high-purity
TW201402599A (en) 2012-05-30 2014-01-16 Suntory Holdings Ltd Steviol glycosyltransferase and gene coding the same
WO2014048392A1 (en) * 2012-09-29 2014-04-03 中国科学院上海生命科学研究院 Method for producing stevioside compounds by microorganism
MX367859B (en) 2012-12-04 2019-09-09 Evolva Sa Methods and materials for biosynthesis of mogroside compounds.
WO2014086890A1 (en) * 2012-12-05 2014-06-12 Evolva Sa Steviol glycoside compositions sensory properties
CN105051195B (en) * 2013-02-06 2019-09-06 埃沃尔瓦公司 Method for improving the generation of Rebaudiodside A D and Rebaudiodside A M
CA2900882A1 (en) * 2013-02-11 2014-08-14 Evolva Sa Efficient production of steviol glycosides in recombinants hosts
KR101404728B1 (en) * 2013-02-28 2014-06-09 씨제이제일제당 (주) A method for preparation of Rebaudioside A from stevioside
MX358413B (en) * 2013-03-15 2018-08-20 Coca Cola Co Steviol glycosides, their compositions and their purification.
DK3004366T3 (en) 2013-05-31 2019-05-20 Dsm Ip Assets Bv MICRO-ORGANISMS FOR DITERPEN PRODUCTION
WO2014191580A1 (en) * 2013-05-31 2014-12-04 Dsm Ip Assets B.V. Extracellular diterpene production
US10952458B2 (en) 2013-06-07 2021-03-23 Purecircle Usa Inc Stevia extract containing selected steviol glycosides as flavor, salty and sweetness profile modifier
MX2015016791A (en) 2013-06-07 2016-09-09 Purecircle Usa Inc Stevia extract containing selected steviol glycosides as flavor, salty and sweetness profile modifier.
KR101559478B1 (en) 2013-06-24 2015-10-13 한국생명공학연구원 Method for Preparing Natural High Intensity Sweetener Rebaudiside A by Using Enzymatic Conversion
CA2914900A1 (en) * 2013-07-15 2015-01-22 Dsm Ip Assets B.V. Recombinant microorganism and process for the production of steviol glycosides
US10273519B2 (en) 2013-07-23 2019-04-30 Dsm Ip Assets B.V. Diterpene production in Yarrowia
MX2016000825A (en) * 2013-07-31 2016-05-24 Dsm Ip Assets Bv Steviol glycosides.
CN108064226A (en) * 2013-07-31 2018-05-22 帝斯曼知识产权资产管理有限公司 The recycling of steviol glycoside
EP3042953A4 (en) * 2013-08-02 2017-07-12 Suntory Holdings Limited Method for using hexenol glycosyl transferase
CN103397064B (en) 2013-08-14 2015-04-15 苏州汉酶生物技术有限公司 Method for preparing rebaudioside M through enzyme method
US20160215306A1 (en) * 2013-08-30 2016-07-28 Evolva Sa Method for producing modified resveratrol
WO2015065650A2 (en) * 2013-11-01 2015-05-07 Conagen Inc. Recombinant production of steviol glycosides
WO2015113569A1 (en) 2014-01-31 2015-08-06 University Of Copenhagen Biosynthesis of forskolin and related compounds
US20180020709A1 (en) * 2014-04-16 2018-01-25 Purecircle Usa Inc. Rebaudioside m biosynthetic production and recovery methods
MX2017001859A (en) 2014-08-11 2017-04-11 Evolva Sa Production of steviol glycosides in recombinant hosts.
CN114794444A (en) 2014-09-02 2022-07-29 谱赛科美国股份有限公司 Stevia extract rich in rebaudioside D, E, N and/or O and preparation method thereof
CN105850727B (en) 2015-01-23 2018-12-07 谱赛科美国公司 The preparation of STEVIA REBAUDIANA new varieties and high RD, RM content steviol glycoside
CN105850750B (en) 2015-01-23 2018-06-12 谱赛科美国公司 STEVIA REBAUDIANA new varieties 814011 compose the preparation of star 3 and high RM contents steviol glycoside
AU2015314251A1 (en) * 2014-09-09 2017-03-16 Evolva Sa Production of steviol glycosides in recombinant hosts
CN107105734A (en) * 2014-09-19 2017-08-29 谱赛科有限责任公司 High-purity steviol glycoside
WO2016048994A2 (en) * 2014-09-23 2016-03-31 President And Fellows Of Harvard College Systems and methods of cell-free protein synthesis in droplets and other compartments
WO2016049531A1 (en) * 2014-09-26 2016-03-31 Purecircle Usa Inc. Single nucleotide polymorphism (snp) markers for stevia
SG11201702123SA (en) 2014-10-01 2017-04-27 Evolva Sa Methods and materials for biosynthesis of mogroside compounds
JP6774945B2 (en) * 2014-10-03 2020-10-28 コナゲン インコーポレイテッド Non-caloric sweeteners and methods for synthesis
WO2016055578A1 (en) * 2014-10-08 2016-04-14 Dsm Ip Assets B.V. Steviol glycoside production
WO2016073740A1 (en) * 2014-11-05 2016-05-12 Manus Biosynthesis, Inc. Microbial production of steviol glycosides
US10208326B2 (en) 2014-11-13 2019-02-19 Evolva Sa Methods and materials for biosynthesis of manoyl oxide
CN109006460B (en) * 2015-01-23 2022-03-22 谱赛科美国公司 Preparation of new stevia rebaudiana variety 817096 Puxing No. 5 and stevioside with high RD content
SG11201705606PA (en) 2015-01-30 2017-08-30 Evolva Sa Production of steviol glycosides in recombinant hosts
CA2979931A1 (en) 2015-03-16 2016-09-22 Dsm Ip Assets B.V. Udp-glycosyltransferases
EP3303601B1 (en) 2015-05-28 2022-05-11 Evolva SA Biosynthesis of phenylpropanoids and phenylpropanoid derivatives
JP2018516600A (en) 2015-05-29 2018-06-28 カーギル・インコーポレイテッド Fermentation process for producing steviol glycosides using high pH and compositions obtained therefrom
JP2018521636A (en) 2015-05-29 2018-08-09 カーギル・インコーポレイテッド Fermentation process for producing steviol glycosides using multi-stage feeding
CN107613786A (en) 2015-05-29 2018-01-19 嘉吉公司 Produce the heat treatment of glucosides
MY192153A (en) * 2015-07-10 2022-08-02 Dsm Ip Assets Bv Steviol glycoside composition
MY192148A (en) 2015-07-10 2022-08-02 Dsm Ip Assets Bv Steviol glycoside composition
BR112018002371A2 (en) 2015-08-06 2019-09-17 Cargill Inc method for the production of steviol glycoside (s)
AU2016307066A1 (en) 2015-08-07 2018-02-08 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3337893B1 (en) 2015-08-21 2023-07-19 Manus Bio Inc. Increasing productivity of e. coli host cells that functionally express p450 enzymes
US9765117B2 (en) * 2015-08-24 2017-09-19 Romek Figa Peptides for treating cancer
BR112018006724A2 (en) 2015-10-05 2018-10-09 Dsm Ip Assets Bv kaurenoic acid hydroxylases
CN108495559A (en) 2015-10-26 2018-09-04 谱赛科美国股份有限公司 steviol glycoside composition
US20200308616A1 (en) 2015-11-30 2020-10-01 Purecircle Sdn Bhd Process for producing high purity steviol glycosides
EP3387136B1 (en) * 2015-12-10 2022-01-05 Evolva SA Production of steviol glycosides in recombinant hosts
RU2764635C2 (en) 2015-12-15 2022-01-19 ПЬЮРСЁРКЛ ЮЭсЭй ИНК. Steviol glycoside composition
EP3426791A1 (en) 2016-03-11 2019-01-16 Evolva SA Production of steviol glycosides in recombinant hosts
AU2017251462B2 (en) 2016-04-13 2022-02-03 Evolva Sa Production of steviol glycosides in recombinant hosts
US20190144907A1 (en) * 2016-05-16 2019-05-16 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3458599A1 (en) 2016-05-16 2019-03-27 Evolva SA Production of steviol glycosides in recombinant hosts
CN116210879A (en) * 2016-06-06 2023-06-06 泰特&莱尔解决方案美国有限责任公司 Glycosylated steviol glycoside compositions and methods of making glycosylated steviol glycoside compositions
EA201990035A1 (en) 2016-06-15 2019-06-28 Кодексис, Инк. GENETIC-ENGINEERING BETA-GLUCOSIDASES AND GLUCOSYLATION METHODS
MY194280A (en) 2016-08-12 2022-11-25 Amyris Inc Udp-dependent glycosyltransferase for high efficiency production of rebaudiosides
JP2019532650A (en) * 2016-10-21 2019-11-14 ペプシコ・インク Method for preparing rebaudioside C by using an enzymatic method
JP6972123B2 (en) 2016-10-21 2021-11-24 ペプシコ・インク A method for preparing rebaudioside N using an enzymatic method
CN116574775A (en) 2016-10-21 2023-08-11 百事可乐公司 Method for preparing rebaudioside J by enzyme method
WO2018083338A1 (en) 2016-11-07 2018-05-11 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3564251A4 (en) * 2016-12-27 2020-12-23 Suntory Holdings Limited Novel steviol glycoside, method for producing same, and sweetener composition containing same
JP7214477B2 (en) * 2016-12-27 2023-01-30 サントリーホールディングス株式会社 Steviol glycoside hexosyltransferase and its encoding gene
CN108341781B (en) * 2017-01-25 2021-09-03 中国科学院分子植物科学卓越创新中心 Method for analyzing related enzymes in biosynthetic pathway of plant secondary metabolites
CN110869500B (en) 2017-01-26 2023-10-27 马努斯生物合成股份有限公司 Metabolic engineering for microbial production of terpenoid products
WO2018144679A2 (en) 2017-02-03 2018-08-09 Codexis, Inc. Engineered glycosyltransferases and steviol glycoside glucosylation methods
JP6878607B2 (en) * 2017-02-21 2021-05-26 デューク ユニバーシティ Compositions and Methods for Robust Dynamic Metabolic Control
SG10202111903TA (en) 2017-03-31 2021-12-30 Suntory Holdings Ltd Novel steviol glycoside and production method therefor, and sweetener composition containing same
JP7011649B2 (en) * 2017-04-12 2022-02-10 サントリーホールディングス株式会社 Stevia-derived rhamnose synthase and genes
WO2018213279A1 (en) * 2017-05-15 2018-11-22 Purecircle Usa Inc. High-purity steviol glycosides
WO2018211032A1 (en) 2017-05-17 2018-11-22 Evolva Sa Production of steviol glycosides in recombinant hosts
US11248248B2 (en) 2017-06-15 2022-02-15 Evolva Sa Production of mogroside compounds in recombinant hosts
JP7487099B2 (en) * 2017-08-11 2024-05-20 アミリス, インコーポレイテッド Pea (Pisum sativum) kaurene oxidase for highly efficient production of rebaudioside
US11365417B2 (en) 2017-09-12 2022-06-21 Bio Capital Holdings, LLC Biological devices and methods of use thereof to produce steviol glycosides
BR112020009838A2 (en) * 2017-12-05 2020-11-17 Evolva Sa production of steviol glycosides in recombinant hosts
US11541105B2 (en) 2018-06-01 2023-01-03 The Research Foundation For The State University Of New York Compositions and methods for disrupting biofilm formation and maintenance
AU2019304965A1 (en) * 2018-07-16 2021-02-11 Manus Bio, Inc. Production of steviol glycosides through whole cell biotransformation
BR112021026306A2 (en) 2019-06-25 2022-09-06 Manus Bio Inc URIDINE DIPHOSPHATE GLYCOSYLTRANSFERASE ENZYME
KR20220044789A (en) 2019-08-08 2022-04-11 도아고세이가부시키가이샤 Water dissolving adhesive composition
CN110592040A (en) * 2019-09-18 2019-12-20 江苏施宇甜生物科技有限公司 Process for producing recombinant bacillus subtilis of UPD (ultra-high Performance) glycosyltransferase
CN111424065B (en) * 2020-03-24 2022-08-16 金禾益康(北京)生物科技有限公司 Method for glycosylating stevioside compounds by using glycosyltransferase
WO2023006179A1 (en) 2020-07-27 2023-02-02 Dsm Ip Assets B.V. Method to produce retinyl acetate
CN112147253B (en) * 2020-09-25 2022-04-01 安徽瑞思威尔科技有限公司 UPC2-PDA-Q-Tof/MS detection method for 42 effective components in ganoderma lucidum wine
WO2022084482A1 (en) 2020-10-22 2022-04-28 Dsm Ip Assets B.V. Microorganisms for diterpene production
CN112877355A (en) * 2021-01-22 2021-06-01 杜云龙 Method for expressing notoginsenoside by using tobacco
WO2022245988A2 (en) * 2021-05-18 2022-11-24 Biokai, Inc. Production of carotenoids
CN115478060B (en) * 2021-06-16 2023-11-28 弈柯莱生物科技(集团)股份有限公司 Glycosyltransferase and application thereof
WO2023006851A1 (en) 2021-07-27 2023-02-02 Dsm Ip Assets B.V. Fermentative production of retinyl acetate in the presence of ethanol
KR20230098493A (en) * 2021-12-24 2023-07-04 주식회사 삼양사 Rebaudioside production
CN114752575B (en) * 2022-04-07 2023-06-13 内蒙古工业大学 NAD (NAD) + Dependent dehydrogenase gene and application thereof in improving coenzyme Q10 yield
CN116004563B (en) * 2023-01-17 2023-11-17 华中农业大学 Tea tree flavonoid glycosyltransferase and application thereof

Family Cites Families (138)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58149697A (en) 1982-02-27 1983-09-06 Dainippon Ink & Chem Inc Preparation of beta-1,3-glycosyl stevioside
JPS59101408A (en) * 1982-12-02 1984-06-12 Junichi Iwamura Plant growth regulator
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US6946587B1 (en) 1990-01-22 2005-09-20 Dekalb Genetics Corporation Method for preparing fertile transgenic corn plants
JPH03277275A (en) 1990-03-28 1991-12-09 Dainippon Ink & Chem Inc Novel enzyme and method for preparing glycoside with same enzyme
US5204253A (en) 1990-05-29 1993-04-20 E. I. Du Pont De Nemours And Company Method and apparatus for introducing biological substances into living cells
US5306862A (en) 1990-10-12 1994-04-26 Amoco Corporation Method and composition for increasing sterol accumulation in higher plants
US5460949A (en) 1990-11-15 1995-10-24 Amoco Corporation Method and composition for increasing the accumulation of squalene and specific sterols in yeast
US5712112A (en) 1992-11-04 1998-01-27 National Science Council Of R.O.C. Gene expression system comprising the promoter region of the alpha-amylase genes
JPH09503905A (en) 1993-07-15 1997-04-22 ネオゼ ファーマシューティカルス Method for synthesizing sugar composition
US7186891B1 (en) 1996-04-12 2007-03-06 University Of Kentucky, Research Foundation Plant cells and plants expressing chimeric isoprenoid synthases
JPH10117776A (en) 1996-10-22 1998-05-12 Japan Tobacco Inc Transformation of indica rice
WO1999018224A1 (en) 1997-10-06 1999-04-15 The Centre National De Recherche Scientifique Plant fatty acid hydroxylase genes
US6255557B1 (en) 1998-03-31 2001-07-03 Her Majesty The Queen In Right Of Canada As Represented By The Ministerof Agriculture And Agri-Food Canada Stevia rebaudiana with altered steviol glycoside composition
US6806076B1 (en) 1998-04-14 2004-10-19 Kyowa Hakko Kogyo Co., Ltd. Process for producing isoprenoid compounds by microorganisms and a method for screening compounds with antibiotic or weeding activity
TWI250210B (en) 1998-05-06 2006-03-01 Dsm Ip Assets Bv An isolated DNA sequence coding for an enzyme involved in the mevalonate pathway or the pathway from isopentenyl pyrophosphate to farnesyl pyrophosphate
EP0955363A3 (en) 1998-05-06 2004-01-28 F. Hoffmann-La Roche Ag Dna sequences encoding enzymes involved in production of isoprenoids
EP2305825B1 (en) 1998-07-06 2015-01-14 DCV Inc. doing business as Bio-Technical Resourses Method of vitamin production
EP1095002A4 (en) 1998-07-06 2005-08-03 Dcv Inc Method of vitamin production
AR021636A1 (en) 1998-12-17 2002-07-31 Rubicon Forests Holdings Ltd MATERIALS AND METHODS FOR THE MODIFICATION OF CONTENT, COMPOSITION AND METABOLISM OF ISOPRENOIDS
JP2002533090A (en) 1998-12-23 2002-10-08 ザ、サミュアル、ラバツ、ノゥブル、ファウンデイシャン、インク Plant transformation method
BR0010616A (en) 1999-04-15 2002-05-07 Calgene Llc Nucleic acid sequences to proteins involved in isoprenoid synthesis
EP1171618A2 (en) 1999-04-21 2002-01-16 The Samuel Roberts Noble Foundation Plant transformation process
EP1198575B1 (en) 1999-08-04 2007-09-19 Adelbert Bacher Isoprenoid biosynthesis
AU6780700A (en) 1999-08-18 2001-03-13 Paradigm Genetics, Inc. Methods and apparatus for transformation of monocotyledenous plants using agrobacterium in combination with vacuum filtration
AU2001269685A1 (en) 2000-05-03 2001-11-12 The Salk Institute For Biological Studies Crystallization of 4-diphosphocytidyl-2-c-methylerythritol synthesis
DE10027821A1 (en) 2000-06-05 2001-12-06 Adelbert Bacher New intermediate in isoprenoid biosynthesis, useful in screening for potential herbicides, comprises mutant encoding-enzymes sequences for imparting herbicide resistance
WO2002010398A2 (en) 2000-07-31 2002-02-07 Hahn Frederick M Manipulation of genes of the mevalonate and isoprenoid pathways to create novel traits in transgenic organisms
EP1313867A2 (en) 2000-08-24 2003-05-28 The Scripps Research Institute Stress-regulated genes of plants, transgenic plants containing same, and methods of use
US6660507B2 (en) 2000-09-01 2003-12-09 E. I. Du Pont De Nemours And Company Genes involved in isoprenoid compound production
US6689601B2 (en) 2000-09-01 2004-02-10 E. I. Du Pont De Nemours And Company High growth methanotropic bacterial strain
US6818424B2 (en) 2000-09-01 2004-11-16 E. I. Du Pont De Nemours And Company Production of cyclic terpenoids
EP1409530A2 (en) 2000-09-19 2004-04-21 Microbia, Inc. Modulation of secondary metabolite production by zinc binuclear cluster proteins
DE60132498T2 (en) 2000-09-29 2009-01-15 Cargill, Inc., Wayzata ISOPRENOIDPRODUKTION
CN1345963A (en) * 2000-09-29 2002-04-24 上海博德基因开发有限公司 Novel polypeptide-UDP glycosyltransferase (UGT) and cobalamin conjugated protein 9.57 and polynucleotide for encoding said polypeptide
US6949362B2 (en) 2000-12-12 2005-09-27 E. I. Du Pont De Nemours And Company Rhodococcus cloning and expression vectors
US7838287B2 (en) 2001-01-25 2010-11-23 Evolva Sa Library of a collection of cells
US20040078846A1 (en) 2002-01-25 2004-04-22 Desouza Mervyn L. Carotenoid biosynthesis
US20050003474A1 (en) 2001-01-26 2005-01-06 Desouza Mervyn L. Carotenoid biosynthesis
DE10201458A1 (en) 2001-04-11 2002-10-17 Adelbert Bacher New proteins involved in isoprenoid biosynthesis, useful in screening for inhibitors, also new intermediates, potential therapeutic agents, nucleic acids and antibodies
US7034140B2 (en) 2001-04-24 2006-04-25 E.I. Du Pont De Nemours And Company Genes involved in isoprenoid compound production
US6989257B2 (en) 2001-06-06 2006-01-24 Dsm Ip Assets B.V. Isoprenoid production
AU2002341541A1 (en) 2001-06-22 2003-03-03 Syngenta Participations Ag Abiotic stress responsive polynucleotides and polypeptides
US20040072311A1 (en) 2001-08-28 2004-04-15 Dicosimo Deana J. Production of cyclic terpenoids
US20040010815A1 (en) 2001-09-26 2004-01-15 Lange B. Markus Identification and characterization of plant genes
US7172886B2 (en) 2001-12-06 2007-02-06 The Regents Of The University Of California Biosynthesis of isopentenyl pyrophosphate
ATE444367T1 (en) 2002-08-20 2009-10-15 Suntory Holdings Ltd NEW GLYCOSYLTRANSFERASE GENES
MXPA05002120A (en) 2002-09-27 2005-06-06 Dsm Ip Assets Bv Squalene synthase 8sqs9 gen.
US7098000B2 (en) 2003-06-04 2006-08-29 E. I. Du Pont De Nemoure And Company Method for production of C30-aldehyde carotenoids
ES2323175T3 (en) 2003-06-12 2009-07-08 Dsm Ip Assets B.V. MEVALONATE-KINASES RESISTANT TO FEEDBACK.
US7569389B2 (en) 2004-09-30 2009-08-04 Ceres, Inc. Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics
MXPA06013502A (en) 2004-05-21 2007-03-01 Univ California Method for enhancing production of isoprenoid compounds.
US20060014264A1 (en) 2004-07-13 2006-01-19 Stowers Institute For Medical Research Cre/lox system with lox sites having an extended spacer region
JPWO2006016395A1 (en) 2004-08-09 2008-05-01 国立大学法人東北大学 UDP-glucuronyltransferase and gene thereof
CN101044243B (en) 2004-08-19 2011-12-28 帝斯曼知识产权资产管理有限公司 Production method of isoprenoids
US7923552B2 (en) 2004-10-18 2011-04-12 SGF Holdings, LLC High yield method of producing pure rebaudioside A
EP1824969B1 (en) 2004-12-14 2011-03-02 DSM IP Assets B.V. Improved mevalonate kinase
CA2598792A1 (en) 2005-03-02 2006-09-08 Metanomics Gmbh Process for the production of fine chemicals
WO2006096392A2 (en) 2005-03-04 2006-09-14 Diversa Corporation Enzymes involved in astaxanthin, carotenoid and isoprenoid biosynthetic pathways, genes encoding them and methods of making and using them
WO2006093289A1 (en) * 2005-03-04 2006-09-08 National Institute Of Advanced Industrial Science And Technology Process for prpduction of udp-xylose
US7989677B2 (en) 2005-08-17 2011-08-02 Nestec S. A. Nucleic acids and proteins associated with sucrose accumulation in coffee
US8293307B2 (en) 2005-10-11 2012-10-23 Purecircle Sdn Bhd Process for manufacturing a sweetener and use thereof
EP1971228A1 (en) 2005-11-23 2008-09-24 The Coca-Cola Company Natural high-potency sweetener compositions with improved temporal profile and/or flavor profile, methods for their formulation, and uses
US20070116833A1 (en) * 2005-11-23 2007-05-24 The Coca-Cola Company High-Potency Sweetener Composition with Calcium and Compositions Sweetened Therewith
US7927851B2 (en) * 2006-03-21 2011-04-19 Vineland Research And Innovation Centre Compositions having ent-kaurenoic acid 13-hydroxylase activity and methods for producing same
EP2090662A3 (en) * 2006-04-05 2012-10-31 Metanomics GmbH Process for the production of a fine chemical
US8114645B2 (en) 2006-05-19 2012-02-14 The Regents Of The University Of California Methods for increasing isoprenoid and isoprenoid precursor production by modulating fatty acid levels
EP2035827B1 (en) 2006-06-19 2012-10-17 Givaudan S.A. Nucleic acid, polypeptide and its use
WO2008008256A2 (en) 2006-07-07 2008-01-17 The Regents Of The University Of California Methods for enhancing production of isoprenoid compounds by host cells
US8257957B2 (en) 2006-09-26 2012-09-04 The Regents Of The University Of California Production of isoprenoids and isoprenoid precursors
US7741119B2 (en) 2006-09-28 2010-06-22 E. I. Du Pont De Nemours And Company Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production
US7629156B2 (en) 2006-09-28 2009-12-08 E.I. Du Pont De Nemours And Company Ethanol production in fermentation of mixed sugars containing xylose
CN101200480B (en) 2006-12-15 2011-03-30 成都华高药业有限公司 Rebaudioside A extraction method
JP4915917B2 (en) 2006-12-22 2012-04-11 独立行政法人農業・食品産業技術総合研究機構 Method for producing lacto-N-biose I and galacto-N-biose
JP5307730B2 (en) 2007-01-22 2013-10-02 カーギル・インコーポレイテッド Process for the preparation of a purified rebaudioside A composition using solvent / antisolvent crystallization
JP2008237110A (en) 2007-03-27 2008-10-09 Institute Of Physical & Chemical Research Steviol synthetic enzyme gene and method for producing steviol
EP2152852A2 (en) 2007-05-17 2010-02-17 Tetravitae Bioscience, Inc. Methods and compositions for producing solvents
US20080292775A1 (en) 2007-05-22 2008-11-27 The Coca-Cola Company Delivery Systems for Natural High-Potency Sweetener Compositions, Methods for Their Formulation, and Uses
WO2009005704A1 (en) 2007-07-03 2009-01-08 The Regents Of The University Of California Methods of increasing isoprenoid or isoprenoid precursor production
JP2009034080A (en) 2007-08-03 2009-02-19 Sanei Gen Ffi Inc New glycosyltransferase and method for producing glycoside by utilizing the same
US7964232B2 (en) 2007-09-17 2011-06-21 Pepsico, Inc. Steviol glycoside isomers
KR101574817B1 (en) 2007-12-03 2015-12-04 디에스엠 아이피 어셋츠 비.브이. Novel nutraceutical compositions containing stevia extract or stevia extract constituents and uses thereof
WO2009086049A2 (en) * 2007-12-27 2009-07-09 Mc Neil Nutritionals, Llc Synergistic sweetening compositions
CA2715397A1 (en) 2008-02-20 2009-08-27 Ceres, Inc. Nucleotide sequences and corresponding polypeptides conferring improved nitrogen use efficiency characteristics in plants
TWI475963B (en) * 2008-02-25 2015-03-11 Coca Cola Co Rebaudioside a derivative products and methods for making
US9005444B2 (en) 2008-05-13 2015-04-14 Cargill, Incorporated Separation of rebaudioside A from stevia glycosides using chromatography
WO2010021001A2 (en) 2008-08-19 2010-02-25 Kaushik Ramakrishnan S Process for preparing sweetener from stevia rebaudiana
KR101918422B1 (en) 2008-10-03 2018-11-13 모리타 가가쿠 고교 가부시키가이샤 New steviol glycoside
CN101720910B (en) 2008-10-23 2012-07-25 大闽食品(漳州)有限公司 Method for preparing stevioside
US8614085B2 (en) 2009-02-27 2013-12-24 Butamax(Tm) Advanced Biofuels Llc Yeast with increased butanol tolerance involving a multidrug efflux pump gene
US20100297722A1 (en) 2009-05-20 2010-11-25 Board Of Trustees Of Southern Illinois University Transgenic moss producing terpenoids
NZ597129A (en) 2009-06-08 2012-12-21 Jennewein Biotechnologie Gmbh Hmo synthesis
US9131718B2 (en) 2009-06-16 2015-09-15 Epc (Beijing) Natural Products Co., Ltd. Process for rebaudioside D
CA2773134A1 (en) 2009-09-04 2011-03-10 Redpoint Bio Corporation Sweetness enhancers including rebaudioside a or d
BR112012006282A2 (en) 2009-09-22 2015-09-01 Redpoint Bio Corp Crystalline form I of rebaudioside c alone, methods of manufacturing it, for purifying rebaudioside c, for enhancing a sweet taste of a carbohydrate sweetener, for decreasing the amount of a carbohydrate sweetener in a consumable product, and for increasing the sweetness of carbohydrate. a consumable product, and consumable product.
US8299224B2 (en) 2009-10-15 2012-10-30 Purecircle Sdn Bhd High-purity Rebaudioside D
EP2358730B1 (en) 2009-10-15 2014-01-15 Purecircle SDN BHD Method for preparing high-purity rebaudioside d
US8703224B2 (en) 2009-11-04 2014-04-22 Pepsico, Inc. Method to improve water solubility of Rebaudioside D
WO2011062748A1 (en) 2009-11-23 2011-05-26 E.I. Du Pont De Nemours And Company Sucrose transporter genes for increasing plant seed lipids
WO2011072246A2 (en) 2009-12-10 2011-06-16 Regents Of The University Of Minnesota Tal effector-mediated dna modification
BR112012016103B1 (en) 2009-12-28 2020-11-10 The Coca-Cola Company sweetening intensifiers, compositions thereof and methods for use
US9441233B2 (en) 2010-05-06 2016-09-13 Ceres, Inc. Transgenic plants having increased biomass
EP2571991A1 (en) 2010-05-20 2013-03-27 Evolva SA Method of producing isoprenoid compounds in yeast
EP2575499A2 (en) 2010-05-31 2013-04-10 Vib Vzw The use of transporters to modulate flavor production by yeast
CN105671108A (en) 2010-06-02 2016-06-15 沃维公司 Recombinant production of steviol glycosides
US20120021111A1 (en) 2010-07-23 2012-01-26 Aexelon Therapeutics, Inc. Natural Low Caloric Sweetener Compositions for Use in Beverages, Foods and Pharmaceuticals, and Their Methods of Manufacture
US20120083593A1 (en) 2010-10-01 2012-04-05 Shanghai Yongyou Bioscience Inc. Separation and Purification of Stevioside and Rebaudioside A
CA2819253A1 (en) 2010-11-30 2012-06-07 Massachusetts Institute Of Technology Microbial production of natural sweeteners, diterpenoid steviol glycosides
KR20130014227A (en) 2011-07-29 2013-02-07 한국생명공학연구원 NOVEL α-GLUCOSYL STEVIOSIDES AND PROCESS FOR PRODUCING THE SAME
EP3009508B1 (en) 2011-08-08 2020-11-25 Evolva SA Recombinant production of steviol glycosides
AU2012342114B2 (en) 2011-11-23 2017-12-21 Evolva Sa Methods and materials for enzymatic synthesis of mogroside compounds
CN103159808B (en) 2011-12-09 2017-03-29 上海泓博智源医药股份有限公司 A kind of process for preparing natural sweetener
ES2971273T3 (en) 2011-12-19 2024-06-04 Coca Cola Co Beverage comprising rebaudioside
CN104203005A (en) 2012-01-23 2014-12-10 帝斯曼知识产权资产管理有限公司 Diterpene production
JP6251669B2 (en) * 2012-03-16 2017-12-20 サントリーホールディングス株式会社 Steviol glycoside enzyme and gene encoding the same
CN110358795A (en) 2012-05-22 2019-10-22 谱赛科有限责任公司 The steviol glycoside of high-purity
WO2014048392A1 (en) 2012-09-29 2014-04-03 中国科学院上海生命科学研究院 Method for producing stevioside compounds by microorganism
WO2014086890A1 (en) 2012-12-05 2014-06-12 Evolva Sa Steviol glycoside compositions sensory properties
CN105051195B (en) 2013-02-06 2019-09-06 埃沃尔瓦公司 Method for improving the generation of Rebaudiodside A D and Rebaudiodside A M
CA2900882A1 (en) 2013-02-11 2014-08-14 Evolva Sa Efficient production of steviol glycosides in recombinants hosts
CN105189176B (en) 2013-02-11 2017-11-07 达纳比利时股份有限公司 hydrostatic and direct drive transmission device
WO2014191580A1 (en) 2013-05-31 2014-12-04 Dsm Ip Assets B.V. Extracellular diterpene production
DK3004366T3 (en) 2013-05-31 2019-05-20 Dsm Ip Assets Bv MICRO-ORGANISMS FOR DITERPEN PRODUCTION
KR101559478B1 (en) 2013-06-24 2015-10-13 한국생명공학연구원 Method for Preparing Natural High Intensity Sweetener Rebaudiside A by Using Enzymatic Conversion
CA2914900A1 (en) 2013-07-15 2015-01-22 Dsm Ip Assets B.V. Recombinant microorganism and process for the production of steviol glycosides
US10273519B2 (en) 2013-07-23 2019-04-30 Dsm Ip Assets B.V. Diterpene production in Yarrowia
CN108064226A (en) 2013-07-31 2018-05-22 帝斯曼知识产权资产管理有限公司 The recycling of steviol glycoside
MX2016000825A (en) 2013-07-31 2016-05-24 Dsm Ip Assets Bv Steviol glycosides.
EP3042953A4 (en) 2013-08-02 2017-07-12 Suntory Holdings Limited Method for using hexenol glycosyl transferase
CN103397064B (en) 2013-08-14 2015-04-15 苏州汉酶生物技术有限公司 Method for preparing rebaudioside M through enzyme method
US20160215306A1 (en) 2013-08-30 2016-07-28 Evolva Sa Method for producing modified resveratrol
WO2015051454A1 (en) 2013-10-07 2015-04-16 Vineland Research And Innovation Centre Compositions and methods for producing steviol and steviol glycosides
EP3114210A2 (en) 2014-03-07 2017-01-11 Evolva SA Methods for recombinant production of saffron compounds
MX2017001859A (en) 2014-08-11 2017-04-11 Evolva Sa Production of steviol glycosides in recombinant hosts.
AU2015314251A1 (en) 2014-09-09 2017-03-16 Evolva Sa Production of steviol glycosides in recombinant hosts
SG11201705606PA (en) 2015-01-30 2017-08-30 Evolva Sa Production of steviol glycosides in recombinant hosts
CN104845990A (en) 2015-06-11 2015-08-19 山东大学 Application of Arabidopsis glycosyltransferase gene UGT73C7 in improving plant disease resistance
AU2016307066A1 (en) 2015-08-07 2018-02-08 Evolva Sa Production of steviol glycosides in recombinant hosts

Also Published As

Publication number Publication date
US20240110215A1 (en) 2024-04-04
EP2575432A1 (en) 2013-04-10
KR101971818B1 (en) 2019-04-23
KR20190043637A (en) 2019-04-26
US20220073960A1 (en) 2022-03-10
US20130171328A1 (en) 2013-07-04
JP2017079739A (en) 2017-05-18
JP6509188B2 (en) 2019-05-08
NZ708078A (en) 2017-01-27
CN103179850A (en) 2013-06-26
US10392644B2 (en) 2019-08-27
MX349121B (en) 2017-07-12
BR112012030836A2 (en) 2019-10-08
KR101802547B1 (en) 2017-11-28
AU2011261394A1 (en) 2013-01-10
JP6110551B1 (en) 2017-04-05
BR122021005297B1 (en) 2022-02-22
BR122021005283B1 (en) 2022-02-22
EP2575432A4 (en) 2014-01-22
KR102181638B1 (en) 2020-11-24
MY185668A (en) 2021-05-28
US9562251B2 (en) 2017-02-07
JP6947772B2 (en) 2021-10-13
CA2802627C (en) 2023-02-07
WO2011153378A1 (en) 2011-12-08
MX2012014016A (en) 2013-10-01
BR112012030836B8 (en) 2024-02-06
CN103179850B (en) 2016-03-16
CA2802627A1 (en) 2011-12-08
US20190078128A9 (en) 2019-03-14
EP3593633A1 (en) 2020-01-15
US20200140912A1 (en) 2020-05-07
MX2020007169A (en) 2020-08-31
CN105671108A (en) 2016-06-15
KR20170131717A (en) 2017-11-29
CA3176307A1 (en) 2011-12-08
NZ604915A (en) 2014-10-31
JP6177127B2 (en) 2017-08-09
AU2011261394C1 (en) 2016-10-27
BR122021005304B1 (en) 2022-02-22
JP2021151238A (en) 2021-09-30
JP2023093503A (en) 2023-07-04
JP2017074058A (en) 2017-04-20
JP2013533736A (en) 2013-08-29
BR112012030836B1 (en) 2021-10-05
SG186127A1 (en) 2013-01-30
CL2012003373A1 (en) 2014-08-08
NZ700097A (en) 2015-10-30
BR122021005287B1 (en) 2022-02-22
AU2011261394B2 (en) 2015-08-27
MY167872A (en) 2018-09-26
KR20130122603A (en) 2013-11-07
US20170218419A1 (en) 2017-08-03
EP2575432B1 (en) 2019-08-14
JP2019146573A (en) 2019-09-05
MY180803A (en) 2020-12-09

Similar Documents

Publication Publication Date Title
SG10201709458QA (en) Recombinant production of steviol glycosides
SG10201606569VA (en) Recombinant production of steviol glycosides
EP2544538A4 (en) High-purity steviol glycosides
EP3063286A4 (en) Recombinant production of steviol glycosides
EP2852296A4 (en) High-purity steviol glycosides
PL2675294T3 (en) Glucosyl stevia composition
EP2720561A4 (en) Stevia composition
EP2675909A4 (en) Clucosyl stevia composition
PL2713763T3 (en) Stevia composition
PL2713762T3 (en) Stevia composition
LT2793618T (en) Beverage comprising steviol glycosides
EP2435577A4 (en) Production of glycoproteins
EP2672840A4 (en) Stevia composition
PL2486806T3 (en) Stevia composition
PL2584918T3 (en) Product comprising stevia
HK1164702A1 (en) Composition with improved bioavailability of saponin
EP2545179A4 (en) Production of polyhydroxyalkanoates
EP2690972A4 (en) Glucosyl stevia composition
EP2768320A4 (en) Glucosyl stevia composition
EP2657244A4 (en) Glycoside compound
PT2542591T (en) Silyl-derivatives of polysaccharides
EP2730176A4 (en) Taste-improving peptide
GB201017450D0 (en) Glycosylation of aminocoumarins
EP2459540A4 (en) Preparation of fipamezole
TWM372743U (en) Improved structure of Kendama