SG11201702123SA - Methods and materials for biosynthesis of mogroside compounds - Google Patents

Methods and materials for biosynthesis of mogroside compounds

Info

Publication number
SG11201702123SA
SG11201702123SA SG11201702123SA SG11201702123SA SG11201702123SA SG 11201702123S A SG11201702123S A SG 11201702123SA SG 11201702123S A SG11201702123S A SG 11201702123SA SG 11201702123S A SG11201702123S A SG 11201702123SA SG 11201702123S A SG11201702123S A SG 11201702123SA
Authority
SG
Singapore
Prior art keywords
biosynthesis
materials
methods
mogroside compounds
mogroside
Prior art date
Application number
SG11201702123SA
Inventor
Jens Houghton-Larsen
Katarzyna Krzystanek
Angelika Semmler
Iver Klavs Riishede Hansen
Soren Damkiaer
Gary Liu
Yaoquan Liu
Jorgen Hansen
Sathish Kumar
Muthuswamy Panchapagesa Murali
Nina Nicoline Rasmussen
Original Assignee
Evolva Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US14/504,109 external-priority patent/US10011859B2/en
Application filed by Evolva Sa filed Critical Evolva Sa
Publication of SG11201702123SA publication Critical patent/SG11201702123SA/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A23L27/36—Terpene glycosides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04J—MULTIPLEX COMMUNICATION
    • H04J14/00—Optical multiplex systems
    • H04J14/02—Wavelength-division multiplex systems
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
SG11201702123SA 2014-10-01 2015-09-30 Methods and materials for biosynthesis of mogroside compounds SG11201702123SA (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US14/504,109 US10011859B2 (en) 2012-12-04 2014-10-01 Methods and materials for biosynthesis of mogroside compounds
US201462059136P 2014-10-02 2014-10-02
US201462087726P 2014-12-04 2014-12-04
US201462090836P 2014-12-11 2014-12-11
US201462091895P 2014-12-15 2014-12-15
US201562199115P 2015-07-30 2015-07-30
PCT/EP2015/072645 WO2016050890A2 (en) 2014-10-01 2015-09-30 Methods and materials for biosynthesis of mogroside compounds

Publications (1)

Publication Number Publication Date
SG11201702123SA true SG11201702123SA (en) 2017-04-27

Family

ID=55631719

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201902813XA SG10201902813XA (en) 2014-10-01 2015-09-30 Methods and materials for biosynthesis of mogroside compounds
SG11201702123SA SG11201702123SA (en) 2014-10-01 2015-09-30 Methods and materials for biosynthesis of mogroside compounds

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG10201902813XA SG10201902813XA (en) 2014-10-01 2015-09-30 Methods and materials for biosynthesis of mogroside compounds

Country Status (9)

Country Link
US (2) US10633685B2 (en)
EP (1) EP3201315A2 (en)
JP (1) JP2017529860A (en)
CN (1) CN107466320B (en)
AU (2) AU2015326892B2 (en)
CA (2) CA3232630A1 (en)
IL (2) IL251380B (en)
SG (2) SG10201902813XA (en)
WO (1) WO2016050890A2 (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL287789B1 (en) 2014-09-11 2024-01-01 The State Of Israel Ministry Of Agriculture & Rural Development Agricultural Res Organization Aro Vo Methods of producing mogrosides and compositions comprising same and uses thereof
CN105801658B (en) * 2014-12-30 2019-03-19 北京农学院 The preparation of sweet tea glucoside and the like and application as STAT3, ERK signal path target site drug in antitumor
AU2016344131A1 (en) 2015-10-29 2018-05-10 Senomyx, Inc. High intensity sweeteners
WO2018083338A1 (en) 2016-11-07 2018-05-11 Evolva Sa Production of steviol glycosides in recombinant hosts
US11060124B2 (en) * 2017-05-03 2021-07-13 Firmenich Incorporated Methods for making high intensity sweeteners
US11248248B2 (en) 2017-06-15 2022-02-15 Evolva Sa Production of mogroside compounds in recombinant hosts
CN109402163A (en) * 2018-09-26 2019-03-01 怀化兴科创生物技术有限公司 The application of new Siraitia grosvenorii squalene epoxidase gene (Sgsqe)
CN109439676A (en) * 2018-09-26 2019-03-08 怀化兴科创生物技术有限公司 The application of Siraitia grosvenorii epoxide hydrolase Sgeph5 gene
CN113316394A (en) 2018-11-07 2021-08-27 弗门尼舍公司 Process for preparing high intensity sweeteners
WO2020096907A1 (en) 2018-11-07 2020-05-14 Firmenich Incorporated Methods for making high intensity sweeteners
CN113302298A (en) * 2018-11-09 2021-08-24 银杏生物制品公司 Biosynthesis of mogrosides
CN110066785B (en) * 2019-01-30 2021-01-26 中国医学科学院药用植物研究所 Momordica grosvenori gourd dienol synthase mutant and construction method and application thereof
CN110357936B (en) * 2019-07-16 2020-10-16 湖南华诚生物资源股份有限公司 Semi-synthesis method of mogroside V
TWI711385B (en) * 2019-09-26 2020-12-01 國立臺灣大學 Method for bioconversion of mogroside extracts into siamenoside i
EP4048782A4 (en) * 2019-10-25 2023-11-22 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
US10894812B1 (en) 2020-09-30 2021-01-19 Alpine Roads, Inc. Recombinant milk proteins
US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants
EP4222167A1 (en) 2020-09-30 2023-08-09 Nobell Foods, Inc. Recombinant milk proteins and food compositions comprising the same
CA3177491A1 (en) * 2021-04-02 2022-10-06 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
JP2024513399A (en) * 2021-04-02 2024-03-25 ギンゴー バイオワークス， インコーポレイテッド Biosynthesis of isoprenoids and their precursors
CN113388590B (en) * 2021-06-07 2022-02-25 山西农业大学 Mutant of cytochrome P450s
EP4314316A2 (en) 2021-06-29 2024-02-07 Firmenich Incorporated Methods for making high intensity sweeteners
CN114854722B (en) * 2022-05-11 2024-01-09 成都普睿法药物研发有限公司 Off-cut beta-1, 3-glucanase of candida mongolica and mutant and application thereof
NL2032683B1 (en) * 2022-07-18 2024-01-26 Sestina Bio Llc Bioproduction of isoprenoids

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BR112014012543A2 (en) 2011-11-23 2017-06-13 Evolva Sa methods and materials for enzymatic synthesis of mogroside compounds
BR112015003329A2 (en) 2012-08-17 2017-09-26 Evolva Sa increased production of terpenes and terpenoids
BR112015013129A2 (en) * 2012-12-04 2017-07-11 Evolva Sa methods and materials for biosynthesis of mogroside compounds

Also Published As

Publication number Publication date
US20200325517A1 (en) 2020-10-15
AU2015326892B2 (en) 2022-03-10
WO2016050890A2 (en) 2016-04-07
US20170247735A1 (en) 2017-08-31
IL276781A (en) 2020-10-29
CA3232630A1 (en) 2016-04-07
US10633685B2 (en) 2020-04-28
CN107466320A (en) 2017-12-12
CA2963300A1 (en) 2016-04-07
EP3201315A2 (en) 2017-08-09
SG10201902813XA (en) 2019-04-29
US11091787B2 (en) 2021-08-17
AU2022204012A1 (en) 2022-06-30
AU2015326892A1 (en) 2017-03-30
JP2017529860A (en) 2017-10-12
CN107466320B (en) 2021-11-05
IL251380A0 (en) 2017-05-29
WO2016050890A3 (en) 2016-06-30
AU2015326892A2 (en) 2017-05-25
IL251380B (en) 2020-09-30

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