SG10201902813XA - Methods and materials for biosynthesis of mogroside compounds - Google Patents
Methods and materials for biosynthesis of mogroside compoundsInfo
- Publication number
- SG10201902813XA SG10201902813XA SG10201902813XA SG10201902813XA SG10201902813XA SG 10201902813X A SG10201902813X A SG 10201902813XA SG 10201902813X A SG10201902813X A SG 10201902813XA SG 10201902813X A SG10201902813X A SG 10201902813XA SG 10201902813X A SG10201902813X A SG 10201902813XA
- Authority
- SG
- Singapore
- Prior art keywords
- methods
- biosynthesis
- materials
- mogroside compounds
- compounds
- Prior art date
Links
- 150000001875 compounds Chemical class 0.000 title abstract 4
- 229930189775 mogroside Natural products 0.000 title abstract 4
- 230000015572 biosynthetic process Effects 0.000 title abstract 2
- 239000000463 material Substances 0.000 title abstract 2
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/30—Artificial sweetening agents
- A23L27/33—Artificial sweetening agents containing sugars or derivatives
- A23L27/36—Terpene glycosides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
- C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
- C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P33/00—Preparation of steroids
-
- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04J—MULTIPLEX COMMUNICATION
- H04J14/00—Optical multiplex systems
- H04J14/02—Wavelength-division multiplex systems
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Nutrition Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Computer Networks & Wireless Communication (AREA)
- Signal Processing (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
METHODS AND MATERIALS FOR BIOSYNTHESIS OF MOGROSIDE COMPOUNDS Methods for recombinant and enzymatic production of mogroside compounds and compositions containing mogroside compounds are provided by this invention. [Figure 3A]
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US14/504,109 US10011859B2 (en) | 2012-12-04 | 2014-10-01 | Methods and materials for biosynthesis of mogroside compounds |
US201462059136P | 2014-10-02 | 2014-10-02 | |
US201462087726P | 2014-12-04 | 2014-12-04 | |
US201462090836P | 2014-12-11 | 2014-12-11 | |
US201462091895P | 2014-12-15 | 2014-12-15 | |
US201562199115P | 2015-07-30 | 2015-07-30 |
Publications (1)
Publication Number | Publication Date |
---|---|
SG10201902813XA true SG10201902813XA (en) | 2019-04-29 |
Family
ID=55631719
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG10201902813XA SG10201902813XA (en) | 2014-10-01 | 2015-09-30 | Methods and materials for biosynthesis of mogroside compounds |
SG11201702123SA SG11201702123SA (en) | 2014-10-01 | 2015-09-30 | Methods and materials for biosynthesis of mogroside compounds |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11201702123SA SG11201702123SA (en) | 2014-10-01 | 2015-09-30 | Methods and materials for biosynthesis of mogroside compounds |
Country Status (9)
Country | Link |
---|---|
US (2) | US10633685B2 (en) |
EP (1) | EP3201315A2 (en) |
JP (1) | JP2017529860A (en) |
CN (1) | CN107466320B (en) |
AU (2) | AU2015326892B2 (en) |
CA (2) | CA3232630A1 (en) |
IL (2) | IL251380B (en) |
SG (2) | SG10201902813XA (en) |
WO (1) | WO2016050890A2 (en) |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL287789B1 (en) | 2014-09-11 | 2024-01-01 | The State Of Israel Ministry Of Agriculture & Rural Development Agricultural Res Organization Aro Vo | Methods of producing mogrosides and compositions comprising same and uses thereof |
CN105801658B (en) * | 2014-12-30 | 2019-03-19 | 北京农学院 | The preparation of sweet tea glucoside and the like and application as STAT3, ERK signal path target site drug in antitumor |
AU2016344131A1 (en) | 2015-10-29 | 2018-05-10 | Senomyx, Inc. | High intensity sweeteners |
WO2018083338A1 (en) | 2016-11-07 | 2018-05-11 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
US11060124B2 (en) * | 2017-05-03 | 2021-07-13 | Firmenich Incorporated | Methods for making high intensity sweeteners |
US11248248B2 (en) | 2017-06-15 | 2022-02-15 | Evolva Sa | Production of mogroside compounds in recombinant hosts |
CN109402163A (en) * | 2018-09-26 | 2019-03-01 | 怀化兴科创生物技术有限公司 | The application of new Siraitia grosvenorii squalene epoxidase gene (Sgsqe) |
CN109439676A (en) * | 2018-09-26 | 2019-03-08 | 怀化兴科创生物技术有限公司 | The application of Siraitia grosvenorii epoxide hydrolase Sgeph5 gene |
CN113316394A (en) | 2018-11-07 | 2021-08-27 | 弗门尼舍公司 | Process for preparing high intensity sweeteners |
WO2020096907A1 (en) | 2018-11-07 | 2020-05-14 | Firmenich Incorporated | Methods for making high intensity sweeteners |
CN113302298A (en) * | 2018-11-09 | 2021-08-24 | 银杏生物制品公司 | Biosynthesis of mogrosides |
CN110066785B (en) * | 2019-01-30 | 2021-01-26 | 中国医学科学院药用植物研究所 | Momordica grosvenori gourd dienol synthase mutant and construction method and application thereof |
CN110357936B (en) * | 2019-07-16 | 2020-10-16 | 湖南华诚生物资源股份有限公司 | Semi-synthesis method of mogroside V |
TWI711385B (en) * | 2019-09-26 | 2020-12-01 | 國立臺灣大學 | Method for bioconversion of mogroside extracts into siamenoside i |
EP4048782A4 (en) * | 2019-10-25 | 2023-11-22 | Ginkgo Bioworks, Inc. | Biosynthesis of mogrosides |
US10894812B1 (en) | 2020-09-30 | 2021-01-19 | Alpine Roads, Inc. | Recombinant milk proteins |
US10947552B1 (en) | 2020-09-30 | 2021-03-16 | Alpine Roads, Inc. | Recombinant fusion proteins for producing milk proteins in plants |
EP4222167A1 (en) | 2020-09-30 | 2023-08-09 | Nobell Foods, Inc. | Recombinant milk proteins and food compositions comprising the same |
CA3177491A1 (en) * | 2021-04-02 | 2022-10-06 | Ginkgo Bioworks, Inc. | Biosynthesis of mogrosides |
JP2024513399A (en) * | 2021-04-02 | 2024-03-25 | ギンゴー バイオワークス, インコーポレイテッド | Biosynthesis of isoprenoids and their precursors |
CN113388590B (en) * | 2021-06-07 | 2022-02-25 | 山西农业大学 | Mutant of cytochrome P450s |
EP4314316A2 (en) | 2021-06-29 | 2024-02-07 | Firmenich Incorporated | Methods for making high intensity sweeteners |
CN114854722B (en) * | 2022-05-11 | 2024-01-09 | 成都普睿法药物研发有限公司 | Off-cut beta-1, 3-glucanase of candida mongolica and mutant and application thereof |
NL2032683B1 (en) * | 2022-07-18 | 2024-01-26 | Sestina Bio Llc | Bioproduction of isoprenoids |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6399321B1 (en) | 1999-08-18 | 2002-06-04 | National Research Council Of Canada | Methods for screening UDP-glucose:glycoprotein glucosyltransferase (UGGT) activity and nucleic acid encoding for UGGT |
EP1510573A1 (en) | 2003-09-01 | 2005-03-02 | Austria Wirtschaftsservice Gesellschaft mit beschränkter Haftung | Method for detoxification of mycotoxins |
US7569389B2 (en) | 2004-09-30 | 2009-08-04 | Ceres, Inc. | Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics |
EP2194140A2 (en) | 2005-03-02 | 2010-06-09 | Metanomics GmbH | Process for the production of fine chemicals |
US8257948B1 (en) | 2011-02-17 | 2012-09-04 | Purecircle Usa | Method of preparing alpha-glucosyl Stevia composition |
US8956677B2 (en) | 2005-11-23 | 2015-02-17 | The Coca-Cola Company | High-potency sweetener composition with glucosamine and compositions sweetened therewith |
RU2008122901A (en) | 2005-11-23 | 2009-12-27 | Дзе Кока-Кола Компани (US) | COMPOSITION OF INTENSIVE SWEETER WITH SAPONIN AND SUGGESTED COMPOSITIONS |
US7927851B2 (en) | 2006-03-21 | 2011-04-19 | Vineland Research And Innovation Centre | Compositions having ent-kaurenoic acid 13-hydroxylase activity and methods for producing same |
WO2008062165A2 (en) | 2006-11-22 | 2008-05-29 | The University Of York | Monoterpenoid modifying enzymes |
WO2008065370A2 (en) | 2006-12-01 | 2008-06-05 | The University Of York | Sesquiterpenoid modifying enzymes |
GB0904639D0 (en) | 2009-03-18 | 2009-04-29 | Univ York | Assay |
BR122021005283B1 (en) | 2010-06-02 | 2022-02-22 | Evolva, Inc | RECOMBINANT HOST COMPRISING RECOMBINANT GENES FOR PRODUCTION OF STEVIOL OR STEVIOL GLYCOSIDE, METHOD FOR PRODUCING STEVIOL, STEVIOL GLYCOSIDE OR STEVIOL GLYCOSIDE COMPOSITION AND METHOD FOR SYNTHESISTING STEVIOL OR STEVIOL GLYCOSIDE |
BR112014012543A2 (en) | 2011-11-23 | 2017-06-13 | Evolva Sa | methods and materials for enzymatic synthesis of mogroside compounds |
BR112015003329A2 (en) | 2012-08-17 | 2017-09-26 | Evolva Sa | increased production of terpenes and terpenoids |
BR112015013129A2 (en) * | 2012-12-04 | 2017-07-11 | Evolva Sa | methods and materials for biosynthesis of mogroside compounds |
-
2015
- 2015-09-30 US US15/511,565 patent/US10633685B2/en active Active
- 2015-09-30 WO PCT/EP2015/072645 patent/WO2016050890A2/en active Application Filing
- 2015-09-30 JP JP2017517783A patent/JP2017529860A/en active Pending
- 2015-09-30 SG SG10201902813XA patent/SG10201902813XA/en unknown
- 2015-09-30 CA CA3232630A patent/CA3232630A1/en active Pending
- 2015-09-30 SG SG11201702123SA patent/SG11201702123SA/en unknown
- 2015-09-30 CA CA2963300A patent/CA2963300A1/en active Granted
- 2015-09-30 CN CN201580062037.XA patent/CN107466320B/en active Active
- 2015-09-30 EP EP15797018.7A patent/EP3201315A2/en active Pending
- 2015-09-30 AU AU2015326892A patent/AU2015326892B2/en active Active
-
2017
- 2017-03-26 IL IL251380A patent/IL251380B/en active IP Right Grant
-
2020
- 2020-03-02 US US16/806,812 patent/US11091787B2/en active Active
- 2020-08-18 IL IL276781A patent/IL276781A/en unknown
-
2022
- 2022-06-09 AU AU2022204012A patent/AU2022204012A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
US20200325517A1 (en) | 2020-10-15 |
AU2015326892B2 (en) | 2022-03-10 |
WO2016050890A2 (en) | 2016-04-07 |
US20170247735A1 (en) | 2017-08-31 |
IL276781A (en) | 2020-10-29 |
CA3232630A1 (en) | 2016-04-07 |
US10633685B2 (en) | 2020-04-28 |
CN107466320A (en) | 2017-12-12 |
CA2963300A1 (en) | 2016-04-07 |
EP3201315A2 (en) | 2017-08-09 |
US11091787B2 (en) | 2021-08-17 |
AU2022204012A1 (en) | 2022-06-30 |
AU2015326892A1 (en) | 2017-03-30 |
SG11201702123SA (en) | 2017-04-27 |
JP2017529860A (en) | 2017-10-12 |
CN107466320B (en) | 2021-11-05 |
IL251380A0 (en) | 2017-05-29 |
WO2016050890A3 (en) | 2016-06-30 |
AU2015326892A2 (en) | 2017-05-25 |
IL251380B (en) | 2020-09-30 |
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