AU2012294452C1 - Recombinant production of steviol glycosides - Google Patents

Recombinant production of steviol glycosides Download PDF

Info

Publication number
AU2012294452C1
AU2012294452C1 AU2012294452A AU2012294452A AU2012294452C1 AU 2012294452 C1 AU2012294452 C1 AU 2012294452C1 AU 2012294452 A AU2012294452 A AU 2012294452A AU 2012294452 A AU2012294452 A AU 2012294452A AU 2012294452 C1 AU2012294452 C1 AU 2012294452C1
Authority
AU
Australia
Prior art keywords
steviol
glucose
seq
rebaudioside
gene encoding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2012294452A
Other languages
English (en)
Other versions
AU2012294452A1 (en
AU2012294452B2 (en
AU2012294452A8 (en
Inventor
Michael DALGAARD MIKKELSEN
Sabina DE ANDRADE PEREIRA TAVARES
Hansen Esben Halkjaer
Jorgen Hansen
Paula M. Hicks
Jens Houghton-Larsen
Michael Naesby
Tange Thomas Ostergaard
Ernesto SIMON
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danstar Ferment AG
Original Assignee
Danstar Ferment AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=46724642&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AU2012294452(C1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Danstar Ferment AG filed Critical Danstar Ferment AG
Publication of AU2012294452A1 publication Critical patent/AU2012294452A1/en
Publication of AU2012294452A8 publication Critical patent/AU2012294452A8/en
Publication of AU2012294452B2 publication Critical patent/AU2012294452B2/en
Priority to AU2017258907A priority Critical patent/AU2017258907A1/en
Publication of AU2012294452C1 publication Critical patent/AU2012294452C1/en
Application granted granted Critical
Priority to AU2020200157A priority patent/AU2020200157B2/en
Priority to AU2022203048A priority patent/AU2022203048B2/en
Priority to AU2024204171A priority patent/AU2024204171B2/en
Assigned to DANSTAR FERMENT AG reassignment DANSTAR FERMENT AG Request for Assignment Assignors: EVOLVA SA
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0032—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with oxygen as acceptor (1.5.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Seasonings (AREA)
AU2012294452A 2011-08-08 2012-08-08 Recombinant production of steviol glycosides Active AU2012294452C1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2017258907A AU2017258907A1 (en) 2011-08-08 2017-11-09 Recombinant production of steviol glycosides
AU2020200157A AU2020200157B2 (en) 2011-08-08 2020-01-09 Recombinant production of steviol glycosides
AU2022203048A AU2022203048B2 (en) 2011-08-08 2022-05-06 Recombinant production of steviol glycosides
AU2024204171A AU2024204171B2 (en) 2011-08-08 2024-06-19 Recombinant production of steviol glycosides

Applications Claiming Priority (13)

Application Number Priority Date Filing Date Title
US201161521084P 2011-08-08 2011-08-08
US201161521203P 2011-08-08 2011-08-08
US201161521051P 2011-08-08 2011-08-08
US61/521,084 2011-08-08
US61/521,203 2011-08-08
US61/521,051 2011-08-08
US201161523487P 2011-08-15 2011-08-15
US61/523,487 2011-08-15
US201161567929P 2011-12-07 2011-12-07
US61/567,929 2011-12-07
US201261603639P 2012-02-27 2012-02-27
US61/603,639 2012-02-27
PCT/US2012/050021 WO2013022989A2 (en) 2011-08-08 2012-08-08 Recombinant production of steviol glycosides

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2017258907A Division AU2017258907A1 (en) 2011-08-08 2017-11-09 Recombinant production of steviol glycosides

Publications (4)

Publication Number Publication Date
AU2012294452A1 AU2012294452A1 (en) 2014-02-06
AU2012294452A8 AU2012294452A8 (en) 2014-03-06
AU2012294452B2 AU2012294452B2 (en) 2017-08-10
AU2012294452C1 true AU2012294452C1 (en) 2017-11-30

Family

ID=46724642

Family Applications (5)

Application Number Title Priority Date Filing Date
AU2012294452A Active AU2012294452C1 (en) 2011-08-08 2012-08-08 Recombinant production of steviol glycosides
AU2017258907A Abandoned AU2017258907A1 (en) 2011-08-08 2017-11-09 Recombinant production of steviol glycosides
AU2020200157A Active AU2020200157B2 (en) 2011-08-08 2020-01-09 Recombinant production of steviol glycosides
AU2022203048A Active AU2022203048B2 (en) 2011-08-08 2022-05-06 Recombinant production of steviol glycosides
AU2024204171A Active AU2024204171B2 (en) 2011-08-08 2024-06-19 Recombinant production of steviol glycosides

Family Applications After (4)

Application Number Title Priority Date Filing Date
AU2017258907A Abandoned AU2017258907A1 (en) 2011-08-08 2017-11-09 Recombinant production of steviol glycosides
AU2020200157A Active AU2020200157B2 (en) 2011-08-08 2020-01-09 Recombinant production of steviol glycosides
AU2022203048A Active AU2022203048B2 (en) 2011-08-08 2022-05-06 Recombinant production of steviol glycosides
AU2024204171A Active AU2024204171B2 (en) 2011-08-08 2024-06-19 Recombinant production of steviol glycosides

Country Status (11)

Country Link
US (5) US9631215B2 (https=)
EP (4) EP2742142B2 (https=)
JP (5) JP6262653B2 (https=)
KR (2) KR20200057799A (https=)
CN (2) CN103732753B (https=)
AU (5) AU2012294452C1 (https=)
BR (4) BR122021015508B1 (https=)
CA (2) CA3128532A1 (https=)
MY (1) MY173212A (https=)
SG (4) SG10201606565XA (https=)
WO (1) WO2013022989A2 (https=)

Families Citing this family (151)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8790730B2 (en) 2005-10-11 2014-07-29 Purecircle Usa Process for manufacturing a sweetener and use thereof
US9107436B2 (en) 2011-02-17 2015-08-18 Purecircle Sdn Bhd Glucosylated steviol glycoside as a flavor modifier
US8257948B1 (en) 2011-02-17 2012-09-04 Purecircle Usa Method of preparing alpha-glucosyl Stevia composition
US9392799B2 (en) 2011-02-17 2016-07-19 Purecircle Sdn Bhd Glucosyl stevia composition
US8318459B2 (en) 2011-02-17 2012-11-27 Purecircle Usa Glucosyl stevia composition
US9386797B2 (en) 2011-02-17 2016-07-12 Purecircle Sdn Bhd Glucosyl stevia composition
MX353094B (es) 2009-11-12 2017-12-19 Purecircle Usa Inc Granulacion de un edulcorante de stevia.
US10888099B2 (en) 2009-11-12 2021-01-12 Purecircle Sdn Bhd. Granulation of a Stevia sweetener
US10696706B2 (en) 2010-03-12 2020-06-30 Purecircle Usa Inc. Methods of preparing steviol glycosides and uses of the same
EP2544538B1 (en) 2010-03-12 2022-06-15 PureCircle USA Inc. High-purity steviol glycosides
US20150344512A1 (en) 2011-12-19 2015-12-03 Purecircle Usa Inc. Methods of purifying steviol glycosides and uses of the same
EP2575432B1 (en) 2010-06-02 2019-08-14 Evolva, Inc. Recombinant production of steviol glycosides
US9029426B2 (en) 2010-12-13 2015-05-12 Purecircle Sdn Bhd Highly soluble Rebaudioside D
US9510611B2 (en) 2010-12-13 2016-12-06 Purecircle Sdn Bhd Stevia composition to improve sweetness and flavor profile
MX362676B (es) 2011-02-10 2019-01-31 Purecircle Usa Composición de estevia.
US9474296B2 (en) 2011-02-17 2016-10-25 Purecircle Sdn Bhd Glucosyl stevia composition
US9603373B2 (en) 2011-02-17 2017-03-28 Purecircle Sdn Bhd Glucosyl stevia composition
US11690391B2 (en) 2011-02-17 2023-07-04 Purecircle Sdn Bhd Glucosylated steviol glycoside as a flavor modifier
US9894922B2 (en) 2011-05-18 2018-02-20 Purecircle Sdn Bhd Glucosyl rebaudioside C
PL2713763T3 (pl) 2011-05-31 2019-10-31 Purecircle Usa Inc Kompozycja stewiowa
ES2579827T3 (es) 2011-06-03 2016-08-17 Purecircle Usa Composición de estevia
BR122019003378B1 (pt) 2011-06-20 2020-07-28 Purecircle Usa Inc composição ingerível doce, produto alimentício ou de bebida, preparado de droga, farmacêutico ou cosmético, edulcorante e produto compreendendo a composição ingerível doce
US9771434B2 (en) 2011-06-23 2017-09-26 Purecircle Sdn Bhd Products from stevia rebaudiana
CA3128532A1 (en) 2011-08-08 2013-02-14 Evolva Sa Recombinant production of steviol glycosides
US10480019B2 (en) 2011-08-10 2019-11-19 Purecircle Sdn Bhd Process for producing high-purity rubusoside
US20140171520A1 (en) 2011-09-07 2014-06-19 Avetik Markosyan Highly soluble stevia sweetener
EP4124245A1 (en) 2011-12-19 2023-02-01 The Coca-Cola Company Beverage comprising rebaudioside x
FR2984743B1 (fr) * 2011-12-23 2014-08-22 Oreal Utilisation cosmetique du steviol, d'un derive glycoside du steviol, ou un de leurs isomeres pour stimuler, restaurer ou reguler le metabolisme des cellules de la peau et des semi-muqueuses.
MX355028B (es) * 2012-01-23 2018-03-28 Dsm Ip Assets Bv Produccion de diterpeno.
US10000783B2 (en) 2012-03-16 2018-06-19 Suntory Holdings Limited Steviol glucosyltransferases and genes encoding the same
WO2013146555A1 (ja) * 2012-03-27 2013-10-03 サントリーホールディングス株式会社 ステビオール配糖体の製造方法
US20130338348A1 (en) * 2012-03-30 2013-12-19 J.R. Simplot Company Steviol and steviol glycoside formation in plants
MX395285B (es) * 2012-05-22 2025-03-25 Purecircle Sdn Bhd Glucosidos de esteviol de alta pureza.
US9752174B2 (en) 2013-05-28 2017-09-05 Purecircle Sdn Bhd High-purity steviol glycosides
EP2928321A1 (en) * 2012-12-05 2015-10-14 Evolva SA Steviol glycoside compositions sensory properties
MY199452A (en) * 2013-02-06 2023-10-30 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
BR112015019160A2 (pt) 2013-02-11 2017-08-22 Dalgaard Mikkelsen Michael Produção de glicosídeos de esteviol em hospedeiros recombinantes
KR101404728B1 (ko) * 2013-02-28 2014-06-09 씨제이제일제당 (주) 스테비오사이드로부터 리바우디오사이드 a를 제조하는 방법
BR112015023476A8 (pt) * 2013-03-15 2019-12-03 Cargill Inc composição, processo para a preparação de uma composição e extrato
EP2996486B1 (en) * 2013-05-15 2019-10-23 Purecircle Usa Inc. Process for obtaining a stevia composition
CA2912347A1 (en) * 2013-05-31 2014-12-04 Dsm Ip Assets B.V. Extracellular diterpene production
MX367033B (es) 2013-05-31 2019-08-02 Dsm Ip Assets Bv Microorganismos para la produccion de diterpeno.
EP3714699B1 (en) 2013-06-07 2024-12-18 PureCircle USA Inc. Stevia extract containing selected steviol glycosides as flavor, salty and sweetness profile modifier
US10952458B2 (en) 2013-06-07 2021-03-23 Purecircle Usa Inc Stevia extract containing selected steviol glycosides as flavor, salty and sweetness profile modifier
US10039834B2 (en) 2013-07-12 2018-08-07 The Coca-Cola Company Compositions and methods using rebaudioside X to provide sweetness enhancement
EP3021689B1 (en) * 2013-07-15 2021-03-24 DSM IP Assets B.V. Diterpene production
EP3024941B1 (en) * 2013-07-23 2019-07-03 DSM IP Assets B.V. Diterpene production in yarrowia
AU2014298430A1 (en) * 2013-07-31 2016-02-11 Dsm Ip Assets B.V. Steviol glycosides
CN103397064B (zh) 2013-08-14 2015-04-15 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙m的方法
AU2014342939B2 (en) * 2013-11-01 2018-03-22 Conagen Inc. Recombinant production of steviol glycosides
CN106471128A (zh) * 2014-01-28 2017-03-01 百事可乐公司 一种酶法制备瑞鲍迪甙m的方法
CN106459880A (zh) * 2014-04-25 2017-02-22 帝斯曼知识产权资产管理有限公司 细胞色素p450还原酶的稳定化
US9522929B2 (en) 2014-05-05 2016-12-20 Conagen Inc. Non-caloric sweetener
CA2957331A1 (en) 2014-08-11 2016-02-18 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3683315A1 (en) * 2014-08-19 2020-07-22 Purecircle SDN BHD Method for preparing rebaudioside m
US11202461B2 (en) 2014-09-02 2021-12-21 Purecircle Sdn Bhd Stevia extracts
CA2960693A1 (en) 2014-09-09 2016-03-17 Evolva Sa Production of steviol glycosides in recombinant hosts
CN104232496B (zh) * 2014-09-18 2017-06-06 广州康琳奈生物科技有限公司 一种重组毕赤酵母工程菌及其在合成莱鲍迪苷a中的应用
EP4166010A1 (en) 2014-09-19 2023-04-19 PureCircle SDN BHD High-purity steviol glycosides
BR112017006163B1 (pt) * 2014-10-03 2022-04-05 Conagen Inc Produto de consumo oral e método para adoçar o mesmo
CN107109453A (zh) * 2014-11-05 2017-08-29 马努斯生物合成股份有限公司 甜菊醇糖苷的微生物产生
CN107249356B (zh) 2014-12-17 2021-08-06 嘉吉公司 用于口服摄入或使用的甜菊醇糖苷化合物、组合物以及用于增强甜菊醇糖苷溶解度的方法
CN108522291B (zh) * 2015-01-23 2022-01-04 谱赛科美国公司 利用甜叶菊新品种814011谱星3号制备高rm含量甜菊糖苷的方法
EP3250686A1 (en) * 2015-01-30 2017-12-06 Evolva SA Production of steviol glycosides in recombinant hosts
MX2017010311A (es) * 2015-02-10 2017-12-14 Purecircle Usa Inc Metodos de produccion y recuperacion de rebaudiosido m biosintetico.
WO2016141207A1 (en) 2015-03-03 2016-09-09 The Regents Of The University Of California Protecting group chemistry for clean, reductant-free dyeing
WO2016146711A1 (en) 2015-03-16 2016-09-22 Dsm Ip Assets B.V. Udp-glycosyltransferases
CA2979957A1 (en) * 2015-03-23 2016-09-29 Dsm Ip Assets B.V. Udp-glycosyltransferases from solanum lycopersicum
CN107666834B (zh) 2015-04-03 2021-08-24 帝斯曼知识产权资产管理有限公司 甜菊醇糖苷
KR102569053B1 (ko) * 2015-04-14 2023-08-21 코나겐 인크. 조작된 전체 세포 촉매를 사용한 비칼로리 감미료의 제조
BR112017022488A2 (pt) * 2015-04-21 2018-07-17 Dsm Ip Assets Bv produção microbiana de terpenoides
EP3303552A4 (en) * 2015-05-29 2019-01-16 Cargill, Incorporated FERMENTATION METHOD FOR THE PRODUCTION OF STEVIOL GLYCOSIDES WITH MULTIPHASE SUPPLY
EP3303553B1 (en) 2015-05-29 2022-07-27 Cargill, Incorporated Fermentation methods for producing steviol glycosides using high ph
US10612065B2 (en) 2015-05-29 2020-04-07 Cargill, Incorporated Heat treatment to produce glycosides
CN105200098A (zh) * 2015-06-30 2015-12-30 苏州汉酶生物技术有限公司 一种利用酿酒酵母酶法制备瑞鲍迪甙m的方法
US10844414B2 (en) 2015-08-06 2020-11-24 Cargill, Incorporated Methods for producing steviol glycosides in engineered yeast
MX2018001620A (es) 2015-08-07 2018-06-11 Evolva Sa Produccion de glucosidos de esteviol en hospedadores recombinantes.
EP3943504A1 (en) * 2015-08-13 2022-01-26 DSM IP Assets B.V. Steviol glycoside transport
US9765117B2 (en) 2015-08-24 2017-09-19 Romek Figa Peptides for treating cancer
CN108138152B (zh) 2015-10-05 2023-01-06 帝斯曼知识产权资产管理有限公司 贝壳杉烯酸羟化酶
US11647771B2 (en) 2015-10-26 2023-05-16 Purecircle Usa Inc. Steviol glycoside compositions
WO2017093895A1 (en) * 2015-11-30 2017-06-08 Purecircle Sdn Bhd Process for producing high purity steviol glycosides
EP3387136B1 (en) * 2015-12-10 2022-01-05 Evolva SA Production of steviol glycosides in recombinant hosts
RU2764635C2 (ru) 2015-12-15 2022-01-19 ПЬЮРСЁРКЛ ЮЭсЭй ИНК. Композиция стевиол гликозида
US20190021382A1 (en) * 2016-03-01 2019-01-24 Wm. Wrigley Jr. Company Long-lasting sweetener formulations
WO2017153538A1 (en) * 2016-03-11 2017-09-14 Evolva Sa Production of steviol glycosides in recombinant hosts
US10982249B2 (en) 2016-04-13 2021-04-20 Evolva Sa Production of steviol glycosides in recombinant hosts
WO2017198681A1 (en) * 2016-05-16 2017-11-23 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3458599A1 (en) * 2016-05-16 2019-03-27 Evolva SA Production of steviol glycosides in recombinant hosts
MY198930A (en) * 2016-06-17 2023-10-03 Cargill Inc Steviol glycoside compositions for oral ingestion or use
PT3497222T (pt) 2016-08-12 2022-02-09 Amyris Inc Glicosiltransferase dependente de udp para produção de eficácia elevada de rebaudiosídeos
BR112019007504B1 (pt) * 2016-10-14 2023-05-09 Conagen Inc Método de preparação de uma composição de glicosídeo de esteviol
CN116574775A (zh) * 2016-10-21 2023-08-11 百事可乐公司 一种酶法制备瑞鲍迪甙j的方法
BR112019008048A2 (pt) 2016-10-21 2019-07-02 Pepsico Inc método para preparar rebaudiosídeo n com o uso de método enzimático
US11312985B2 (en) 2016-10-21 2022-04-26 Pepsico, Inc. Enzymatic method for preparing Rebaudioside C
CN109890961B (zh) * 2016-10-27 2024-03-08 帝斯曼知识产权资产管理有限公司 香叶基香叶基焦磷酸酯合酶
WO2018083338A1 (en) 2016-11-07 2018-05-11 Evolva Sa Production of steviol glycosides in recombinant hosts
WO2018111194A1 (en) * 2016-12-16 2018-06-21 Temasek Life Sciences Laboratory Limited Targets for improving terpene production in rhodosporidium toruloides
EP3564369A4 (en) 2016-12-27 2020-07-29 Suntory Holdings Limited STEVIOL GLYCOSIDE HEXOSE TRANSFERASE AND CODING GENE FOR THIS
KR102905512B1 (ko) * 2017-02-03 2025-12-29 테이트 앤드 라일 솔루션스 유에스에이 엘엘씨 조작된 글리코실트랜스퍼라제 및 스테비올 글리코시드 글루코실화 방법
AU2017402700A1 (en) * 2017-03-06 2019-10-17 Conagen Inc. Biosynthetic production of steviol glycoside rebaudioside D4 from rebaudioside E
WO2018165330A1 (en) * 2017-03-08 2018-09-13 Purecircle Usa Inc. High rebaudioside m stevia plant cultivars and methods of producing the same
CN110494564B (zh) * 2017-04-12 2024-03-15 三得利控股株式会社 来自甜菊的鼠李糖合成酶及基因
WO2018211032A1 (en) 2017-05-17 2018-11-22 Evolva Sa Production of steviol glycosides in recombinant hosts
CN110809408B (zh) * 2017-06-30 2024-09-10 科纳根公司 通过β-葡糖苷酶水解甜菊醇糖苷
CA3071029A1 (en) 2017-08-11 2019-02-14 Amyris, Inc. Pisum sativum kaurene oxidase for high efficiency production of rebaudiosides
WO2019055325A2 (en) 2017-09-12 2019-03-21 Biocapital Holdings, Llc BIOOGICAL DEVICES AND METHODS OF USING THE SAME TO PRODUCE STEVIOL GLYCOSIDES
CN107446874B (zh) * 2017-09-25 2020-07-28 四川盈嘉合生科技有限公司 重组大肠杆菌及其在合成莱鲍迪苷d中的用途
BR112020009838A2 (pt) 2017-12-05 2020-11-17 Evolva Sa produção de glicosídeos de esteviol em hospedeiros recombinantes
CN111868252B (zh) * 2018-03-12 2025-06-03 科纳根公司 甜菊糖苷莱苞迪苷j和莱苞迪苷n的生物合成生产
US11541105B2 (en) 2018-06-01 2023-01-03 The Research Foundation For The State University Of New York Compositions and methods for disrupting biofilm formation and maintenance
MY204735A (en) * 2018-07-16 2024-09-11 Manus Bio Inc Production of steviol glycosides through whole cell biotransformation
IL280302B2 (en) 2018-07-30 2025-09-01 Codexis Inc Engineered glycosyltransferases and methods for glycosylation of steviol glycoside
CN109393426A (zh) * 2018-09-29 2019-03-01 四川盈嘉合生科技有限公司 一种复合甜味剂
CA3114953A1 (en) 2018-10-15 2020-04-23 Amyris, Inc. Stevia rebaudiana kaurenoic acid hydroxylase variants for high efficiency production of rebaudiosides
EP3877519B1 (en) 2018-11-09 2026-03-11 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
WO2020117792A2 (en) * 2018-12-04 2020-06-11 The Regents Of The University Of California Scalable biosynthesis of the seaweed neurochemical kainic acid
WO2020123877A1 (en) * 2018-12-12 2020-06-18 Conagen Inc. Biosynthetic production of variant steviol glycosides
KR102618015B1 (ko) * 2018-12-21 2023-12-26 주식회사 삼양사 스테비올 또는 이의 전구체의 생산
SG11202107656TA (en) 2019-01-24 2021-08-30 Amyris Inc Abc transporters for the high efficiency production of rebaudiosides
CN109913380B (zh) * 2019-03-25 2021-12-10 南京工业大学 生产(-)-α-红没药醇的重组解脂耶氏酵母菌及其构建方法和应用
MX2021013124A (es) 2019-05-03 2022-01-24 Amyris Inc Variantes del ácido kaurenoico 13-hidroxilasa (kah) y usos del mismo.
US12065685B1 (en) 2019-05-13 2024-08-20 Amyris, Inc. UDP-glycosyltransferase variants and uses thereof
WO2020264179A1 (en) * 2019-06-25 2020-12-30 Manus Bio, Inc. Uridine diphosphate-dependent glycosyltransferase enzyme
KR102379608B1 (ko) * 2019-10-29 2022-03-29 주식회사 삼양사 개선된 스테비올 배당체 화합물 또는 이의 전구체의 생산
CN111088176B (zh) * 2019-12-30 2022-03-25 北京化工大学 产β-胡萝卜素的基因工程菌及其应用
CN112006035B (zh) * 2020-07-24 2021-07-30 江西新龙生物科技股份有限公司 用于防治植物病害的微生物源杀菌剂及其制备方法
WO2023006179A1 (en) 2020-07-27 2023-02-02 Dsm Ip Assets B.V. Method to produce retinyl acetate
US20250290081A1 (en) 2020-10-22 2025-09-18 Dsm Ip Assets B.V. Microorganisms for diterpene production
CA3200689A1 (en) * 2020-11-24 2022-06-02 Manus Bio Inc. Glycoside product biosynthesis and recovery
JP7210626B2 (ja) * 2021-02-18 2023-01-23 ペプシコ・インク 酵素的方法を使用してレバウディオサイドｊを調製するための方法
US11299700B1 (en) 2021-02-19 2022-04-12 Acequia Biotechnology, Llc Bioreactor containers and methods of growing hairy roots using the same
WO2022187819A1 (en) 2021-03-01 2022-09-09 Cargill, Incorporated Xylosylated steviol glycosides and enzymatic methods for production
KR102600520B1 (ko) * 2021-06-09 2023-11-09 씨제이제일제당 주식회사 제라닐제라닐 피로포스페이트 신타아제 변이체 및 이를 이용한 테트라테르펜, 이의 전구체, 및 테트라테르펜을 전구체로 하는 물질의 생산방법
CN115478060B (zh) * 2021-06-16 2023-11-28 弈柯莱生物科技(集团)股份有限公司 一种糖基转移酶及其应用
WO2023006851A1 (en) 2021-07-27 2023-02-02 Dsm Ip Assets B.V. Fermentative production of retinyl acetate in the presence of ethanol
KR102564619B1 (ko) 2021-08-10 2023-08-07 이재준 사각육면 밀폐형 접이식 스퀘어박스
WO2023044368A1 (en) * 2021-09-17 2023-03-23 Doublerainbow Biosciences Inc. Method for producing glycosylated therapeutics by using an immobilized enzyme preparation
CN113930377B (zh) * 2021-10-14 2023-12-26 广西大学 一株全细胞催化提高莱鲍迪苷a含量的工程菌
CN115975827A (zh) * 2021-10-15 2023-04-18 中国科学院分子植物科学卓越创新中心 用于甜茶素生产的宿主细胞及方法
CN114196696B (zh) * 2021-12-06 2023-10-20 天津大学 一种与特定短肽标签融合能高效催化Reb M生成的重组酶
WO2023122649A1 (en) * 2021-12-21 2023-06-29 Mozhdehi Davoud Temperature-responsive nano-biomaterials from genetically encoded farnesylated proteins
CN114561310B (zh) * 2022-03-17 2022-12-02 江南大学 一种生产甜茶苷的酿酒酵母及其应用
US20260009063A1 (en) * 2022-07-07 2026-01-08 Purecircle Sdn. Bhd. High-purity steviol glycosides
WO2024011263A2 (en) * 2022-07-08 2024-01-11 Cibus Us Llc Producing sesquiterpenes and other terpenes using plant-based biomasses
WO2024042405A1 (en) 2022-08-26 2024-02-29 Amyris Bio Products Portugal, Unipessoal, Ltda. Compositions and methods for the synthesis of bio-based polymers
WO2024042486A1 (en) 2022-08-26 2024-02-29 Amyris Bio Products Portugal, Unipessoal, Ltda. Compositions and methods for the production of polyurethanes
EP4627091A1 (en) * 2022-11-30 2025-10-08 Velozbio Company Compositions and methods for protein production
WO2024116153A1 (en) 2022-12-02 2024-06-06 Amyris Bio Products Portugal, Unipessoal, Ltda. Compositions and methods for using previously cultured cells
CN118755690B (zh) * 2024-09-06 2024-11-19 四川盈嘉合生科技有限公司 一种蔗糖合成酶及其在生物合成甜菊糖苷中的应用
CN119265154B (zh) * 2024-12-10 2025-05-13 诸城市浩天药业有限公司 一种糖基转移酶ugt76g4突变体及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100297722A1 (en) * 2009-05-20 2010-11-25 Board Of Trustees Of Southern Illinois University Transgenic moss producing terpenoids

Family Cites Families (145)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58149697A (ja) 1982-02-27 1983-09-06 Dainippon Ink & Chem Inc β−１，３グリコシルステビオシドの製造方法
JPS59101408A (ja) 1982-12-02 1984-06-12 Junichi Iwamura 植物生長調整剤
US5198360A (en) 1990-01-19 1993-03-30 Eli Lilly And Company Dna sequence conferring a plaque inhibition phenotype
US6946587B1 (en) 1990-01-22 2005-09-20 Dekalb Genetics Corporation Method for preparing fertile transgenic corn plants
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
JPH03277275A (ja) 1990-03-28 1991-12-09 Dainippon Ink & Chem Inc 新規酵素及びその酵素を用いた配糖体の製造方法
US5204253A (en) 1990-05-29 1993-04-20 E. I. Du Pont De Nemours And Company Method and apparatus for introducing biological substances into living cells
US5306862A (en) 1990-10-12 1994-04-26 Amoco Corporation Method and composition for increasing sterol accumulation in higher plants
US5460949A (en) 1990-11-15 1995-10-24 Amoco Corporation Method and composition for increasing the accumulation of squalene and specific sterols in yeast
US5712112A (en) 1992-11-04 1998-01-27 National Science Council Of R.O.C. Gene expression system comprising the promoter region of the alpha-amylase genes
WO1995002683A1 (en) 1993-07-15 1995-01-26 Neose Pharmaceuticals Method of synthesizing saccharide compositions
US7186891B1 (en) 1996-04-12 2007-03-06 University Of Kentucky, Research Foundation Plant cells and plants expressing chimeric isoprenoid synthases
JPH10117776A (ja) 1996-10-22 1998-05-12 Japan Tobacco Inc インディカイネの形質転換方法
WO1999018224A1 (en) 1997-10-06 1999-04-15 The Centre National De Recherche Scientifique Plant fatty acid hydroxylase genes
US6255557B1 (en) 1998-03-31 2001-07-03 Her Majesty The Queen In Right Of Canada As Represented By The Ministerof Agriculture And Agri-Food Canada Stevia rebaudiana with altered steviol glycoside composition
EP2175025A1 (en) 1998-04-14 2010-04-14 Kyowa Hakko Bio Co., Ltd. Process for producing isoprenoid compounds by using microorganisms
EP0955363A3 (en) 1998-05-06 2004-01-28 F. Hoffmann-La Roche Ag Dna sequences encoding enzymes involved in production of isoprenoids
TWI250210B (en) 1998-05-06 2006-03-01 Dsm Ip Assets Bv An isolated DNA sequence coding for an enzyme involved in the mevalonate pathway or the pathway from isopentenyl pyrophosphate to farnesyl pyrophosphate
JP4579415B2 (ja) 1998-07-06 2010-11-10 ディーシーブイ・インコーポレイテッド・ドゥーイング・ビジネス・アズ・バイオ−テクニカル・リソーシィズ ビタミン製造方法
US6531303B1 (en) 1998-07-06 2003-03-11 Arkion Life Sciences Llc Method of producing geranylgeraniol
JP3277275B2 (ja) 1998-07-28 2002-04-22 有限会社シャトウバッグ バッグ等に用いる模様付き表地材及びその製造方法
AR021636A1 (es) 1998-12-17 2002-07-31 Rubicon Forests Holdings Ltd Materiales y metodos para la modificacion del contenido, la composicion y el metabolismo de los isoprenoides
AU2594300A (en) 1998-12-23 2000-07-12 Samuel Roberts Noble Foundation, Inc., The Plant transformation process
MXPA01010488A (es) 1999-04-15 2003-07-21 Calgene Llc Secuencias de acidos nucleicos para proteinas que intervienen en sintesis isoprenoide.
NZ513993A (en) 1999-04-21 2001-09-28 Samuel Roberts Noble Found Inc Plant transformation process
ATE373715T1 (de) 1999-08-04 2007-10-15 Adelbert Bacher Isoprenoid biosynthese
AU6780700A (en) 1999-08-18 2001-03-13 Paradigm Genetics, Inc. Methods and apparatus for transformation of monocotyledenous plants using agrobacterium in combination with vacuum filtration
WO2001083769A2 (en) 2000-05-03 2001-11-08 The Salk Institute For Biological Studies Crystallization of 4-diphosphocytidyl-2-c-methylerythritol synthesis
DE10027821A1 (de) 2000-06-05 2001-12-06 Adelbert Bacher Der Mevalonat-unabhängige Isoprenoidbiosyntheseweg
WO2002010398A2 (en) 2000-07-31 2002-02-07 Hahn Frederick M Manipulation of genes of the mevalonate and isoprenoid pathways to create novel traits in transgenic organisms
US7109033B2 (en) 2000-08-24 2006-09-19 The Scripps Research Institute Stress-regulated genes of plants, transgenic plants containing same, and methods of use
US6818424B2 (en) 2000-09-01 2004-11-16 E. I. Du Pont De Nemours And Company Production of cyclic terpenoids
US6660507B2 (en) 2000-09-01 2003-12-09 E. I. Du Pont De Nemours And Company Genes involved in isoprenoid compound production
US6689601B2 (en) 2000-09-01 2004-02-10 E. I. Du Pont De Nemours And Company High growth methanotropic bacterial strain
AU2001291114A1 (en) 2000-09-19 2002-04-02 Microbia, Inc. Modulation of secondary metabolite production by zinc binuclear cluster proteins
AU9635901A (en) 2000-09-29 2002-04-08 Cargill Inc Isoprenoid production
US6949362B2 (en) 2000-12-12 2005-09-27 E. I. Du Pont De Nemours And Company Rhodococcus cloning and expression vectors
DE60239386D1 (de) 2001-01-25 2011-04-21 Evolva Ltd Zellbibliothek
US20040078846A1 (en) 2002-01-25 2004-04-22 Desouza Mervyn L. Carotenoid biosynthesis
US20050003474A1 (en) 2001-01-26 2005-01-06 Desouza Mervyn L. Carotenoid biosynthesis
DE10201458A1 (de) 2001-04-11 2002-10-17 Adelbert Bacher Intermediate und Enzyme des Mevalonat-unabhängigen Isoprenoidbiosyntheseweg
US7034140B2 (en) 2001-04-24 2006-04-25 E.I. Du Pont De Nemours And Company Genes involved in isoprenoid compound production
AU2002316966B2 (en) 2001-06-06 2007-05-17 Dsm Ip Assets B.V. Improved isoprenoid production
EP1402042A2 (en) * 2001-06-22 2004-03-31 Syngenta Participations AG Abiotic stress responsive polynucleotides and polypeptides
NZ513755A (en) 2001-08-24 2001-09-28 Ann Rachel Holmes Protein expression system in yeast comprising a vector encoding a heterologous membrane protein and its application in screening for drugs
US20040072311A1 (en) 2001-08-28 2004-04-15 Dicosimo Deana J. Production of cyclic terpenoids
US20040010815A1 (en) 2001-09-26 2004-01-15 Lange B. Markus Identification and characterization of plant genes
US7172886B2 (en) 2001-12-06 2007-02-06 The Regents Of The University Of California Biosynthesis of isopentenyl pyrophosphate
JP2005185101A (ja) * 2002-05-30 2005-07-14 National Institute Of Agrobiological Sciences 植物の全長ｃＤＮＡおよびその利用
DE60329505D1 (de) 2002-08-20 2009-11-12 Suntory Holdings Ltd Neue glycosyltransferase-gene
ES2305501T3 (es) 2002-09-27 2008-11-01 Dsm Ip Assets B.V. Gen escualeno sintasa (sqs).
US7098000B2 (en) 2003-06-04 2006-08-29 E. I. Du Pont De Nemoure And Company Method for production of C30-aldehyde carotenoids
US7422884B2 (en) 2003-06-12 2008-09-09 Dsm Ip Assetts B.V. Feedback-resistant mevalonate kinases
US7569389B2 (en) 2004-09-30 2009-08-04 Ceres, Inc. Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics
US20060150283A1 (en) 2004-02-13 2006-07-06 Nickolai Alexandrov Sequence-determined DNA fragments and corresponding polypeptides encoded thereby
ATE537269T1 (de) 2004-05-21 2011-12-15 Univ California Verfahren zur verbesserung der herstellung von isoprenoid-zusammensetzungen
AU2005321630A1 (en) 2004-07-02 2006-07-06 Metanomics Gmbh Process for the production of fine chemicals
US20060014264A1 (en) 2004-07-13 2006-01-19 Stowers Institute For Medical Research Cre/lox system with lox sites having an extended spacer region
JPWO2006016395A1 (ja) 2004-08-09 2008-05-01 国立大学法人東北大学 Ｕｄｐ−グルクロニル基転移酵素およびその遺伝子
JP5074185B2 (ja) 2004-08-19 2012-11-14 ディーエスエム アイピー アセッツ ビー．ブイ． イソプレノイドの生成
US7923552B2 (en) 2004-10-18 2011-04-12 SGF Holdings, LLC High yield method of producing pure rebaudioside A
US8338155B2 (en) 2004-12-14 2012-12-25 Dsm Ip Assets B.V. Modified mevalonate kinase with reduced feedback inhibition
JP4284562B2 (ja) 2005-03-04 2009-06-24 独立行政法人産業技術総合研究所 Ｕｄｐ−キシロースの製造方法
WO2006096392A2 (en) 2005-03-04 2006-09-14 Diversa Corporation Enzymes involved in astaxanthin, carotenoid and isoprenoid biosynthetic pathways, genes encoding them and methods of making and using them
US7989677B2 (en) 2005-08-17 2011-08-02 Nestec S. A. Nucleic acids and proteins associated with sucrose accumulation in coffee
CA2624230A1 (en) 2005-08-22 2007-03-01 Co2 Boost Llc A device and process to generate co2 used for indoor crop production and underwater gardening
US8293307B2 (en) 2005-10-11 2012-10-23 Purecircle Sdn Bhd Process for manufacturing a sweetener and use thereof
AU2006318781B2 (en) 2005-11-23 2012-11-01 The Coca-Cola Company Natural high-potency sweetener compositions with improved temporal profile and/or flavor profile, methods for their formulation, and uses
KR100784261B1 (ko) 2006-01-02 2007-12-11 한국과학기술원 탄저균의 포자외막 단백질을 이용한 목적단백질의 미생물표면발현방법
US7927851B2 (en) 2006-03-21 2011-04-19 Vineland Research And Innovation Centre Compositions having ent-kaurenoic acid 13-hydroxylase activity and methods for producing same
EP2090662A3 (en) 2006-04-05 2012-10-31 Metanomics GmbH Process for the production of a fine chemical
US8114645B2 (en) 2006-05-19 2012-02-14 The Regents Of The University Of California Methods for increasing isoprenoid and isoprenoid precursor production by modulating fatty acid levels
EP2035827B1 (en) 2006-06-19 2012-10-17 Givaudan S.A. Nucleic acid, polypeptide and its use
WO2008008256A2 (en) 2006-07-07 2008-01-17 The Regents Of The University Of California Methods for enhancing production of isoprenoid compounds by host cells
EP2066778B1 (en) 2006-09-26 2016-01-27 The Regents of The University of California Production of isoprenoids and isoprenoid precursors
US7629156B2 (en) 2006-09-28 2009-12-08 E.I. Du Pont De Nemours And Company Ethanol production in fermentation of mixed sugars containing xylose
US7741119B2 (en) 2006-09-28 2010-06-22 E. I. Du Pont De Nemours And Company Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production
CN101200480B (zh) 2006-12-15 2011-03-30 成都华高药业有限公司 莱鲍迪甙a的提取方法
JP4915917B2 (ja) * 2006-12-22 2012-04-11 独立行政法人農業・食品産業技術総合研究機構 ラクト−ｎ−ビオースｉ及びガラクト−ｎ−ビオースの製造方法
ES2381892T3 (es) 2007-01-22 2012-06-01 Cargill, Incorporated Procedimiento para producir composiciones de rebaudiósido A purificado que utiliza la cristalización disolvente/antidisolvente
JP2008237110A (ja) 2007-03-27 2008-10-09 Institute Of Physical & Chemical Research ステビオール合成酵素遺伝子及びステビオールの製造方法
WO2008144060A2 (en) 2007-05-17 2008-11-27 Tetravitae Bioscience, Inc. Methods and compositions for producing solvents
US20080292775A1 (en) 2007-05-22 2008-11-27 The Coca-Cola Company Delivery Systems for Natural High-Potency Sweetener Compositions, Methods for Their Formulation, and Uses
WO2009005704A1 (en) 2007-07-03 2009-01-08 The Regents Of The University Of California Methods of increasing isoprenoid or isoprenoid precursor production
JP2009034080A (ja) 2007-08-03 2009-02-19 Sanei Gen Ffi Inc 新規糖転移酵素、及びそれを利用した配糖体の製造
US7964232B2 (en) 2007-09-17 2011-06-21 Pepsico, Inc. Steviol glycoside isomers
DE112008002456T5 (de) 2007-09-21 2011-01-13 Basf Plant Science Gmbh Pflanzen mit erhöhtem Ertrag
MY155722A (en) 2007-12-03 2015-11-30 Dsm Ip Assets Bv Novel nutraceutical compositions containing stevia extract or stevia extract constituents and uses thereof
WO2009086049A2 (en) 2007-12-27 2009-07-09 Mc Neil Nutritionals, Llc Synergistic sweetening compositions
BRPI0907786B1 (pt) 2008-02-20 2021-06-01 Ceres, Inc. Método de produção de uma planta e método de aumentar o nível de tolerância a baixo teor de nitrogênio em uma planta
TWI475963B (zh) 2008-02-25 2015-03-11 可口可樂公司 甜菊糖苷ａ衍生性產物以及製造彼的方法
CA2728285A1 (en) 2008-03-03 2009-09-11 Joule Unlimited, Inc. Engineered co2 fixing microorganisms producing carbon-based products of interest
WO2009140394A1 (en) 2008-05-13 2009-11-19 Cargill, Incorporated Separation of rebaudioside a from stevia glycosides using chromatography
CN101314776B (zh) * 2008-07-11 2010-11-03 南京农业大学 二磷酸尿核甘葡萄糖基转移酶基因及其所编码的蛋白质
WO2010021001A2 (en) 2008-08-19 2010-02-25 Kaushik Ramakrishnan S Process for preparing sweetener from stevia rebaudiana
ES2592377T3 (es) 2008-10-03 2016-11-29 Morita Kagaku Kogyo Co., Ltd. Nuevos glucósidos de esteviol
CN101720910B (zh) 2008-10-23 2012-07-25 大闽食品（漳州）有限公司 一种甜菊糖甙的制备方法
US8614085B2 (en) 2009-02-27 2013-12-24 Butamax(Tm) Advanced Biofuels Llc Yeast with increased butanol tolerance involving a multidrug efflux pump gene
DK2440661T3 (en) 2009-06-08 2018-03-12 Jennewein Biotechnologie Gmbh HMO synthesis
KR20120027363A (ko) 2009-06-16 2012-03-21 이피씨 (베이징) 내추럴 프로덕츠 컴퍼니, 리미티드 뒷맛을 감소시키거나 제거하기 위한 레바우디오사이드 ｄ를 포함하는 조성물 및 그의 제조 방법
CN102573521B (zh) * 2009-09-04 2014-04-09 红点生物公司 包括莱鲍迪苷a或d的甜度增强剂
AU2010298437B2 (en) 2009-09-22 2014-06-19 Redpoint Bio Corporation Novel polymorphs of rebaudioside C and methods for making and using the same
CA3015571C (en) 2009-10-15 2021-07-06 Purecircle Sdn Bhd High-purity rebaudioside d and applications
US8299224B2 (en) 2009-10-15 2012-10-30 Purecircle Sdn Bhd High-purity Rebaudioside D
US8703224B2 (en) 2009-11-04 2014-04-22 Pepsico, Inc. Method to improve water solubility of Rebaudioside D
WO2011062748A1 (en) 2009-11-23 2011-05-26 E.I. Du Pont De Nemours And Company Sucrose transporter genes for increasing plant seed lipids
HUE041436T2 (hu) 2009-12-10 2019-05-28 Univ Minnesota Tal-effektor-közvetített DNS-módosítás
WO2011090709A1 (en) 2009-12-28 2011-07-28 The Coca-Cola Company Sweetness enhancers, compositions thereof, and methods for use
KR101244315B1 (ko) 2010-10-19 2013-03-14 이화여자대학교 산학협력단 에탄올―저항성 효모 유전자 및 이의 용도
WO2011140329A1 (en) 2010-05-06 2011-11-10 Ceres, Inc. Transgenic plants having increased biomass
WO2011146833A1 (en) 2010-05-20 2011-11-24 Evolva Inc. Method of producing isoprenoid compounds in yeast
US9249420B2 (en) 2010-05-31 2016-02-02 Vib Vzw Isobutanol production using yeasts with modified transporter expression
EP2575432B1 (en) 2010-06-02 2019-08-14 Evolva, Inc. Recombinant production of steviol glycosides
US20120021111A1 (en) 2010-07-23 2012-01-26 Aexelon Therapeutics, Inc. Natural Low Caloric Sweetener Compositions for Use in Beverages, Foods and Pharmaceuticals, and Their Methods of Manufacture
US20120083593A1 (en) 2010-10-01 2012-04-05 Shanghai Yongyou Bioscience Inc. Separation and Purification of Stevioside and Rebaudioside A
EP2645847B1 (en) * 2010-11-30 2018-01-17 Massachusetts Institute of Technology Microbial production of natural sweeteners, diterpenoid steviol glycosides
KR20130014227A (ko) 2011-07-29 2013-02-07 한국생명공학연구원 신규한 α-글루코실 스테비오사이드 및 이의 제조 방법
CA3128532A1 (en) * 2011-08-08 2013-02-14 Evolva Sa Recombinant production of steviol glycosides
BR112014012543A2 (pt) 2011-11-23 2017-06-13 Evolva Sa métodos e materiais para síntese enzimática de compostos de mogrosídeo
CN103159808B (zh) 2011-12-09 2017-03-29 上海泓博智源医药股份有限公司 一种制备天然甜味剂的工艺方法
EP4124245A1 (en) 2011-12-19 2023-02-01 The Coca-Cola Company Beverage comprising rebaudioside x
MX355028B (es) 2012-01-23 2018-03-28 Dsm Ip Assets Bv Produccion de diterpeno.
CN102559528B (zh) * 2012-02-09 2013-08-21 南京工业大学 一种产甜叶菊糖基转移酶ugt76g1的基因工程菌及其应用
US10000783B2 (en) 2012-03-16 2018-06-19 Suntory Holdings Limited Steviol glucosyltransferases and genes encoding the same
MX395285B (es) 2012-05-22 2025-03-25 Purecircle Sdn Bhd Glucosidos de esteviol de alta pureza.
CN103710318B (zh) 2012-09-29 2017-01-18 中国科学院上海生命科学研究院 利用微生物生产甜菊糖苷类化合物的方法
EP2928321A1 (en) 2012-12-05 2015-10-14 Evolva SA Steviol glycoside compositions sensory properties
MY199452A (en) 2013-02-06 2023-10-30 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
BR112015019160A2 (pt) 2013-02-11 2017-08-22 Dalgaard Mikkelsen Michael Produção de glicosídeos de esteviol em hospedeiros recombinantes
CA2912347A1 (en) 2013-05-31 2014-12-04 Dsm Ip Assets B.V. Extracellular diterpene production
KR101559478B1 (ko) 2013-06-24 2015-10-13 한국생명공학연구원 효소전환법을 이용한 천연 고감미료의 제조방법
EP3024941B1 (en) 2013-07-23 2019-07-03 DSM IP Assets B.V. Diterpene production in yarrowia
AU2014298430A1 (en) 2013-07-31 2016-02-11 Dsm Ip Assets B.V. Steviol glycosides
EP3536697A1 (en) 2013-07-31 2019-09-11 DSM IP Assets B.V. Recovery of steviol glycosides
WO2015016393A1 (ja) 2013-08-02 2015-02-05 サントリーホールディングス株式会社 ヘキセノール配糖体化酵素の利用方法
CN103397064B (zh) 2013-08-14 2015-04-15 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙m的方法
US20160215306A1 (en) 2013-08-30 2016-07-28 Evolva Sa Method for producing modified resveratrol
SG10201807693YA (en) 2014-03-07 2018-10-30 Evolva Sa Methods for recombinant production of saffron compounds
CA2957331A1 (en) 2014-08-11 2016-02-18 Evolva Sa Production of steviol glycosides in recombinant hosts
CA2960693A1 (en) 2014-09-09 2016-03-17 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3250686A1 (en) 2015-01-30 2017-12-06 Evolva SA Production of steviol glycosides in recombinant hosts
WO2016146711A1 (en) 2015-03-16 2016-09-22 Dsm Ip Assets B.V. Udp-glycosyltransferases
MX2018001620A (es) 2015-08-07 2018-06-11 Evolva Sa Produccion de glucosidos de esteviol en hospedadores recombinantes.
EP3458599A1 (en) 2016-05-16 2019-03-27 Evolva SA Production of steviol glycosides in recombinant hosts
WO2018083338A1 (en) 2016-11-07 2018-05-11 Evolva Sa Production of steviol glycosides in recombinant hosts

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100297722A1 (en) * 2009-05-20 2010-11-25 Board Of Trustees Of Southern Illinois University Transgenic moss producing terpenoids

Also Published As

Publication number Publication date
JP2014524247A (ja) 2014-09-22
AU2024204171B2 (en) 2026-02-26
EP2742142B2 (en) 2020-03-25
SG10201606565XA (en) 2016-10-28
US12416034B2 (en) 2025-09-16
US10435730B2 (en) 2019-10-08
EP2742142B1 (en) 2016-06-01
WO2013022989A3 (en) 2013-07-04
JP6262653B2 (ja) 2018-01-17
BR112014003037B1 (pt) 2022-04-05
CA2843606C (en) 2021-10-05
WO2013022989A2 (en) 2013-02-14
EP2742142A2 (en) 2014-06-18
SG10201606567TA (en) 2016-10-28
JP7803999B2 (ja) 2026-01-21
KR102114493B1 (ko) 2020-05-26
AU2024204171A1 (en) 2024-07-11
AU2022203048A1 (en) 2022-05-26
JP2024112815A (ja) 2024-08-21
US20200123583A1 (en) 2020-04-23
CN108396044A (zh) 2018-08-14
US20220228185A1 (en) 2022-07-21
AU2012294452A1 (en) 2014-02-06
KR20200057799A (ko) 2020-05-26
CA2843606A1 (en) 2013-02-14
SG10201606563PA (en) 2016-10-28
BR122021015509B1 (pt) 2022-03-29
AU2022203048B2 (en) 2024-03-28
EP3009508A1 (en) 2016-04-20
AU2017258907A1 (en) 2017-11-30
BR122021015508B1 (pt) 2022-03-29
AU2020200157A1 (en) 2020-01-30
EP4726050A2 (en) 2026-04-15
JP2018019715A (ja) 2018-02-08
AU2012294452B2 (en) 2017-08-10
KR20140057322A (ko) 2014-05-12
CA3128532A1 (en) 2013-02-14
US20230407360A1 (en) 2023-12-21
US20170321238A1 (en) 2017-11-09
AU2020200157B2 (en) 2022-02-10
SG10201606569VA (en) 2016-09-29
AU2012294452A8 (en) 2014-03-06
EP3792350A1 (en) 2021-03-17
JP7057765B2 (ja) 2022-04-20
EP3009508B1 (en) 2020-11-25
JP2019205445A (ja) 2019-12-05
US20140329281A1 (en) 2014-11-06
JP2022101592A (ja) 2022-07-06
EP3792350B1 (en) 2026-03-25
US9631215B2 (en) 2017-04-25
CN103732753A (zh) 2014-04-16
BR112014003037A2 (pt) 2018-09-04
BR122020019161B1 (pt) 2022-04-05
MY173212A (en) 2020-01-06
JP6554518B2 (ja) 2019-07-31
CN103732753B (zh) 2018-03-30

Similar Documents

Publication Publication Date Title
US12416034B2 (en) Recombinant production of steviol glycosides
JP6110551B1 (ja) ステビオールグリコシドの組換え生成
AU2015261617C1 (en) Recombinant production of steviol glycosides

Legal Events

Date Code Title Description
TH Corrigenda

Free format text: IN VOL 28 , NO 5 , PAGE(S) 718 UNDER THE HEADING PCT APPLICATIONS THAT HAVE ENTERED THE NATIONAL PHASE - NAME INDEX UNDER THE NAME EVOLVA SA., APPLICATION NO.2012294452, UNDER INID (72) CORRECT THE COINVENTOR TO HANSEN, JORGEN

DA2 Applications for amendment section 104

Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 17 AUG 2017

DA2 Applications for amendment section 104

Free format text: THE NATURE OF THE AMENDMENT IS: AMEND THE NAME OF THE INVENTOR TO READ HOUGHTON-LARSEN, JENS; HICKS, PAULA M.; NAESBY, MICHAEL; OSTERGAARD, TANGE THOMAS; HANSEN, JORGEN; DALGAARD MIKKELSEN, MICHAEL; HALKJAER, HANSEN ESBEN; SIMON, ERNESTO AND DE ANDRADE PEREIRA TAVARES, SABINA

DA3 Amendments made section 104

Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 17 AUG 2017

FGA Letters patent sealed or granted (standard patent)
PC Assignment registered

Owner name: DANSTAR FERMENT AG

Free format text: FORMER OWNER(S): EVOLVA SA