AU2012294452C1 - Recombinant production of steviol glycosides - Google Patents

Recombinant production of steviol glycosides Download PDF

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Publication number
AU2012294452C1
AU2012294452C1 AU2012294452A AU2012294452A AU2012294452C1 AU 2012294452 C1 AU2012294452 C1 AU 2012294452C1 AU 2012294452 A AU2012294452 A AU 2012294452A AU 2012294452 A AU2012294452 A AU 2012294452A AU 2012294452 C1 AU2012294452 C1 AU 2012294452C1
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Australia
Prior art keywords
steviol
glucose
seq
rebaudioside
gene encoding
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Active
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AU2012294452A
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AU2012294452A1 (en
AU2012294452A8 (en
AU2012294452B2 (en
Inventor
Michael DALGAARD MIKKELSEN
Sabina DE ANDRADE PEREIRA TAVARES
Hansen Esben Halkjaer
Jorgen Hansen
Paula M. Hicks
Jens Houghton-Larsen
Michael Naesby
Tange Thomas Ostergaard
Ernesto SIMON
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Danstar Ferment AG
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Danstar Ferment AG
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Application filed by Danstar Ferment AG filed Critical Danstar Ferment AG
Publication of AU2012294452A1 publication Critical patent/AU2012294452A1/en
Publication of AU2012294452A8 publication Critical patent/AU2012294452A8/en
Publication of AU2012294452B2 publication Critical patent/AU2012294452B2/en
Priority to AU2017258907A priority Critical patent/AU2017258907A1/en
Publication of AU2012294452C1 publication Critical patent/AU2012294452C1/en
Application granted granted Critical
Priority to AU2020200157A priority patent/AU2020200157B2/en
Priority to AU2022203048A priority patent/AU2022203048B2/en
Priority to AU2024204171A priority patent/AU2024204171B2/en
Assigned to DANSTAR FERMENT AG reassignment DANSTAR FERMENT AG Request for Assignment Assignors: EVOLVA SA
Active legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0032—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with oxygen as acceptor (1.5.3)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Life Sciences & Earth Sciences (AREA)
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  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
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  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Seasonings (AREA)
AU2012294452A 2011-08-08 2012-08-08 Recombinant production of steviol glycosides Active AU2012294452C1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2017258907A AU2017258907A1 (en) 2011-08-08 2017-11-09 Recombinant production of steviol glycosides
AU2020200157A AU2020200157B2 (en) 2011-08-08 2020-01-09 Recombinant production of steviol glycosides
AU2022203048A AU2022203048B2 (en) 2011-08-08 2022-05-06 Recombinant production of steviol glycosides
AU2024204171A AU2024204171B2 (en) 2011-08-08 2024-06-19 Recombinant production of steviol glycosides

Applications Claiming Priority (13)

Application Number Priority Date Filing Date Title
US201161521051P 2011-08-08 2011-08-08
US201161521084P 2011-08-08 2011-08-08
US201161521203P 2011-08-08 2011-08-08
US61/521,203 2011-08-08
US61/521,051 2011-08-08
US61/521,084 2011-08-08
US201161523487P 2011-08-15 2011-08-15
US61/523,487 2011-08-15
US201161567929P 2011-12-07 2011-12-07
US61/567,929 2011-12-07
US201261603639P 2012-02-27 2012-02-27
US61/603,639 2012-02-27
PCT/US2012/050021 WO2013022989A2 (en) 2011-08-08 2012-08-08 Recombinant production of steviol glycosides

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2017258907A Division AU2017258907A1 (en) 2011-08-08 2017-11-09 Recombinant production of steviol glycosides

Publications (4)

Publication Number Publication Date
AU2012294452A1 AU2012294452A1 (en) 2014-02-06
AU2012294452A8 AU2012294452A8 (en) 2014-03-06
AU2012294452B2 AU2012294452B2 (en) 2017-08-10
AU2012294452C1 true AU2012294452C1 (en) 2017-11-30

Family

ID=46724642

Family Applications (5)

Application Number Title Priority Date Filing Date
AU2012294452A Active AU2012294452C1 (en) 2011-08-08 2012-08-08 Recombinant production of steviol glycosides
AU2017258907A Abandoned AU2017258907A1 (en) 2011-08-08 2017-11-09 Recombinant production of steviol glycosides
AU2020200157A Active AU2020200157B2 (en) 2011-08-08 2020-01-09 Recombinant production of steviol glycosides
AU2022203048A Active AU2022203048B2 (en) 2011-08-08 2022-05-06 Recombinant production of steviol glycosides
AU2024204171A Active AU2024204171B2 (en) 2011-08-08 2024-06-19 Recombinant production of steviol glycosides

Family Applications After (4)

Application Number Title Priority Date Filing Date
AU2017258907A Abandoned AU2017258907A1 (en) 2011-08-08 2017-11-09 Recombinant production of steviol glycosides
AU2020200157A Active AU2020200157B2 (en) 2011-08-08 2020-01-09 Recombinant production of steviol glycosides
AU2022203048A Active AU2022203048B2 (en) 2011-08-08 2022-05-06 Recombinant production of steviol glycosides
AU2024204171A Active AU2024204171B2 (en) 2011-08-08 2024-06-19 Recombinant production of steviol glycosides

Country Status (11)

Country Link
US (5) US9631215B2 (https=)
EP (4) EP4726050A2 (https=)
JP (5) JP6262653B2 (https=)
KR (2) KR102114493B1 (https=)
CN (2) CN108396044A (https=)
AU (5) AU2012294452C1 (https=)
BR (4) BR122021015508B1 (https=)
CA (2) CA2843606C (https=)
MY (1) MY173212A (https=)
SG (4) SG10201606569VA (https=)
WO (1) WO2013022989A2 (https=)

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