MX2019015404A - Hidrólisis de glucósidos de esteviol por beta-glucosidasa. - Google Patents

Hidrólisis de glucósidos de esteviol por beta-glucosidasa.

Info

Publication number
MX2019015404A
MX2019015404A MX2019015404A MX2019015404A MX2019015404A MX 2019015404 A MX2019015404 A MX 2019015404A MX 2019015404 A MX2019015404 A MX 2019015404A MX 2019015404 A MX2019015404 A MX 2019015404A MX 2019015404 A MX2019015404 A MX 2019015404A
Authority
MX
Mexico
Prior art keywords
reb
cells
steviol glycosides
rebaudioside
glucosidase
Prior art date
Application number
MX2019015404A
Other languages
English (en)
Inventor
Guohong Mao
Xiaodan Yu
Jacob Edward Vick
Michael Batten
Original Assignee
Conagen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Conagen Inc filed Critical Conagen Inc
Publication of MX2019015404A publication Critical patent/MX2019015404A/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/25—Removal of unwanted matter, e.g. deodorisation or detoxification using enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A23L27/36—Terpene glycosides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/20—Carbocyclic rings
    • C07H15/24—Condensed ring systems having three or more rings
    • C07H15/256—Polyterpene radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01015—Polygalacturonase (3.2.1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Seasonings (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente descripción proporciona un método para producir cantidades mejoradas de rebaudiósido M (Reb M) a partir de diversos glucósidos de esteviol utilizando la actividad hidrolítica de la beta-glucosidasa. Más específicamente, la presente descripción proporciona un proceso mejorado para producir glucósidos de esteviol deseados a partir de células recombinantes alteradas (por ejemplo, células microbianas recombinantes) que contienen sustratos de interés. Aquí es donde tales células (por ejemplo, microbios) se han modificado para transportar genes capaces de generar glucósidos de esteviol de interés, incluido el rebaudiósido A (Reb A), el rebaudiósido E (Reb E) y/o el esteviósido. En esta modalidad, el proceso comprende las etapas de obtener el material de células alteradas (por ejemplo, células microbianas) y llevar a cabo una hidrólisis enzimática en los esteviósidos presentes en dicho producto de desecho para mejorar la producción de Reb M. Además se proporcionan composiciones de glucósido de esteviol producidas por los métodos descritos aquí.
MX2019015404A 2017-06-30 2018-06-29 Hidrólisis de glucósidos de esteviol por beta-glucosidasa. MX2019015404A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762527482P 2017-06-30 2017-06-30
PCT/US2018/040193 WO2019006244A1 (en) 2017-06-30 2018-06-29 HYDROLYSIS OF STEVIOL GLYCOSIDES BY BETA-GLUCOSIDASE

Publications (1)

Publication Number Publication Date
MX2019015404A true MX2019015404A (es) 2020-07-20

Family

ID=64742225

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2019015404A MX2019015404A (es) 2017-06-30 2018-06-29 Hidrólisis de glucósidos de esteviol por beta-glucosidasa.

Country Status (10)

Country Link
US (1) US11414689B2 (es)
EP (1) EP3644758A4 (es)
JP (1) JP7116751B2 (es)
KR (1) KR20200024789A (es)
CN (1) CN110809408A (es)
AU (1) AU2018294258A1 (es)
BR (1) BR112019027992B1 (es)
CA (1) CA3068440A1 (es)
MX (1) MX2019015404A (es)
WO (1) WO2019006244A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112017005656A2 (pt) 2014-09-19 2018-07-31 Purecircle Sdn Bhd glicosídeos de esteviol de alta pureza
CA3039105C (en) 2016-10-14 2024-03-05 Conagen Inc. Biosynthetic production of steviol glycosides and processes therefore
JP7432533B2 (ja) * 2018-03-16 2024-02-16 ピュアサークル ユーエスエー インコーポレイテッド 高純度ステビオール配糖体
KR20200133250A (ko) * 2018-03-16 2020-11-26 퓨어써클 유에스에이 잉크. 고-순도 스테비올 글리코사이드
CN110564658B (zh) * 2019-09-06 2021-08-17 广西大学 一株大肠杆菌工程菌及其全细胞催化生产甜菊醇的方法
CN113930377B (zh) * 2021-10-14 2023-12-26 广西大学 一株全细胞催化提高莱鲍迪苷a含量的工程菌

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8507022B2 (en) 2009-10-15 2013-08-13 Purecircle Sdn Bhd High-purity rebaudioside D and low-calorie carbonated lemon-flavored beverage containing the same
WO2013022989A2 (en) * 2011-08-08 2013-02-14 Evolva Sa Recombinant production of steviol glycosides
WO2013026151A1 (en) * 2011-08-19 2013-02-28 Justbio Inc. Improved stevia rebaudiana extract and formulation, and uses thereof
US9752174B2 (en) 2013-05-28 2017-09-05 Purecircle Sdn Bhd High-purity steviol glycosides
CN102925518A (zh) * 2012-10-31 2013-02-13 江南大学 一种用甜菊苷制备甜菊双糖苷的方法
AU2014214004B2 (en) 2013-02-06 2018-05-24 Evolva Sa Methods for improved production of Rebaudioside D and Rebaudioside M
EP2996486B1 (en) * 2013-05-15 2019-10-23 Purecircle Usa Inc. Process for obtaining a stevia composition
RU2658361C2 (ru) 2013-06-19 2018-06-21 Конаджен Инк. Ребаудиозид е и пищевые продукты, подслащенные ребаудиозидом е
CN103397064B (zh) 2013-08-14 2015-04-15 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙m的方法
AU2014342939B2 (en) 2013-11-01 2018-03-22 Conagen Inc. Recombinant production of steviol glycosides
US9522929B2 (en) 2014-05-05 2016-12-20 Conagen Inc. Non-caloric sweetener
EP2957182A1 (en) * 2014-06-18 2015-12-23 Technische Hochschule Mittelhessen Improved natural sweetener compositions
BR112017005656A2 (pt) * 2014-09-19 2018-07-31 Purecircle Sdn Bhd glicosídeos de esteviol de alta pureza
WO2016054534A1 (en) 2014-10-03 2016-04-07 Conagen Inc. Non-caloric sweeteners and methods for synthesizing
CN107109453A (zh) 2014-11-05 2017-08-29 马努斯生物合成股份有限公司 甜菊醇糖苷的微生物产生
US10058112B2 (en) * 2014-11-21 2018-08-28 Eco Sweeteners Llc Sweetener composition including enzymatically processed stevia and method of manufacturing
US10364450B2 (en) 2015-01-30 2019-07-30 Evolva Sa Production of steviol glycoside in recombinant hosts
US10844414B2 (en) * 2015-08-06 2020-11-24 Cargill, Incorporated Methods for producing steviol glycosides in engineered yeast
CN105255971B (zh) * 2015-10-27 2018-10-23 江南大学 双酶法制备甜菊单糖苷
CN108473995B (zh) * 2015-12-10 2022-10-18 埃沃尔瓦公司 在重组宿主中产生甜菊醇糖苷
JP6824975B2 (ja) * 2016-05-25 2021-02-03 サントリーホールディングス株式会社 Ａｏｂｇｌ１１ホモログを用いたステビオールおよびステビオール配糖体の製造方法
CA3039105C (en) * 2016-10-14 2024-03-05 Conagen Inc. Biosynthetic production of steviol glycosides and processes therefore
RU2764803C2 (ru) 2017-03-06 2022-01-21 Конаджен Инк. Биосинтетическое получение стевиолового гликозида ребаудиозида d4 из ребаудиозида e
US20220042060A1 (en) 2017-09-08 2022-02-10 Conagen Inc. Biosynthesis production of steviol glycosides and processes therefore

Also Published As

Publication number Publication date
AU2018294258A1 (en) 2020-02-06
US20200291441A1 (en) 2020-09-17
CN110809408A (zh) 2020-02-18
US11414689B2 (en) 2022-08-16
BR112019027992B1 (pt) 2024-02-27
RU2020102899A3 (es) 2021-11-09
EP3644758A1 (en) 2020-05-06
CA3068440A1 (en) 2019-01-03
JP7116751B2 (ja) 2022-08-10
RU2020102899A (ru) 2021-07-30
JP2020526185A (ja) 2020-08-31
BR112019027992A2 (pt) 2020-07-07
KR20200024789A (ko) 2020-03-09
WO2019006244A1 (en) 2019-01-03
EP3644758A4 (en) 2021-03-31

Similar Documents

Publication Publication Date Title
MX2019015404A (es) Hidrólisis de glucósidos de esteviol por beta-glucosidasa.
WO2018144679A3 (en) Engineered glycosyltransferases and steviol glycoside glucosylation methods
BR112012030836B8 (pt) Hospedeiro recombinante que compreende genes recombinantes para produção de esteviol ou glicosídeo de esteviol, método para produzir esteviol, glicosídeo de esteviol ou composição de glicosídeo de esteviol e método para sintetizar esteviol ou glicosídeo de esteviol
MX2019001631A (es) Glicosiltransferasa dependiente de uridina difosfato para la produccion de alta eficiencia de rebaudiosidos.
BR112018002371A2 (pt) método para a produção de glicosídeo(s) de esteviol
MX2017004248A (es) Edulcorantes no caloricos y metodos para sintetizar.
MX2021001260A (es) Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol.
MX337550B (es) Metodos para tratar mezclas de glicosidos para obtener uno o mas de estos glicosidos en forma mas pura.
MX2021000605A (es) Producción de glucósidos de esteviol a través de biotransformación de células enteras.
MX2016000452A (es) Produccion de diterpeno.
MY161071A (en) Methods of producing isoprene and a co-product
BR112017025614A2 (pt) método para produzir glicosídeo de esteviol e composição
MY175283A (en) Mutant microorganisms having high ability to produce putrescine and method for producing putrescine using the same
AU2018271755A1 (en) Method for culturing natural killer cell, using transformed T cell
IN2012DN02509A (es)
WO2013092567A3 (en) Production of 1,3-dienes by enzymatic conversion of 3-hydroxyalk-4-enoates and/or 3-phosphonoxyalk-4-enoates
MX2019009135A (es) Ingenieria metabolica para la produccion microbiana de productos terpenoides.
MX2019004368A (es) Produccion biosintetica de glicosidos de esteviol y procesos de los mismos.
UA106372C2 (en) Enzymatic complex
GB201009882D0 (en) Yeast strains modified in their sophorolipid production and uses thereof
MY168657A (en) Fermentation process for producing chemicals
AU2015344324A8 (en) Endoxylanase mutant, enzyme composition for biomass decomposition, and method for producing sugar solution
Huang et al. Chemical catalysis triggered self-assembly for the bottom-up fabrication of peptide nanofibers and hydrogels
PH12021550797A1 (en) Stevia rebaudiana kaurenoic acid hydroxylase variants for high efficiency production of rebaudiosides
MX2022011627A (es) Nuevo sistema y métodos de producción de mogrósidos.