MX2019001631A - Glicosiltransferasa dependiente de uridina difosfato para la produccion de alta eficiencia de rebaudiosidos. - Google Patents

Glicosiltransferasa dependiente de uridina difosfato para la produccion de alta eficiencia de rebaudiosidos.

Info

Publication number
MX2019001631A
MX2019001631A MX2019001631A MX2019001631A MX2019001631A MX 2019001631 A MX2019001631 A MX 2019001631A MX 2019001631 A MX2019001631 A MX 2019001631A MX 2019001631 A MX2019001631 A MX 2019001631A MX 2019001631 A MX2019001631 A MX 2019001631A
Authority
MX
Mexico
Prior art keywords
udp
host cell
glycosyltransferase
nucleotide sequence
sequence encoding
Prior art date
Application number
MX2019001631A
Other languages
English (en)
Inventor
Zhao Lishan
Li Wenzong
Wichmann Gale
Khankhoje Aditi
Garcia De Gonzalo Chantal
Mahatdejkul-Meadows Tina
Jackson Shaina
Leavell Michael
Platt Darren
Original Assignee
Amyris Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amyris Inc filed Critical Amyris Inc
Publication of MX2019001631A publication Critical patent/MX2019001631A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A23L2/60—Sweeteners
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A23L27/36—Terpene glycosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01017—Glucuronosyltransferase (2.4.1.17)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00—Food ingredients
    • A23V2250/24—Non-sugar sweeteners
    • A23V2250/262—Stevioside

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Nutrition Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Seasonings (AREA)

Abstract

En el presente documento se proveen composiciones y métodos para la producción mejorada de glucósidos de esteviol en una célula hospedera. En algunas modalidades, la célula hospedera es modificada genéticamente para comprender una secuencia de nucleótidos heteróloga que codifica una UDP-glicosiltransferasa 40087 de Setaria italica o su UDP-glicosiltransferasa variante. En algunas modalidades, la célula hospedera es modificada genéticamente para comprender una secuencia de nucleótidos heteróloga que codifica una UDP-glicosiltransferasa sr.UGT_9252778, Bd_UGT10850 y/u Ob_UGT91B1_like. En algunas modalidades, la célula hospedera comprende además una o más secuencias de nucleótidos heterólogas que codifican enzimas adicionales de una vía capaz de producir glucósidos de esteviol en la célula hospedera. Las composiciones y métodos descritos en el presente documento proveen una vía eficaz para la producción heteróloga de glucósidos de esteviol, que incluyen, pero sin limitarse a, rebaudiósido D y rebaudiósido M.
MX2019001631A 2016-08-12 2017-08-11 Glicosiltransferasa dependiente de uridina difosfato para la produccion de alta eficiencia de rebaudiosidos. MX2019001631A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662374408P 2016-08-12 2016-08-12
PCT/US2017/046637 WO2018031955A2 (en) 2016-08-12 2017-08-11 Udp-dependent glycosyltransferase for high efficiency production of rebaudiosides

Publications (1)

Publication Number Publication Date
MX2019001631A true MX2019001631A (es) 2019-08-05

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
MX2019001631A MX2019001631A (es) 2016-08-12 2017-08-11 Glicosiltransferasa dependiente de uridina difosfato para la produccion de alta eficiencia de rebaudiosidos.

Country Status (14)

Country Link
US (3) US11091743B2 (es)
EP (1) EP3497222B1 (es)
JP (2) JP7404068B2 (es)
KR (1) KR102331018B1 (es)
CN (1) CN109804073B (es)
AU (1) AU2017308143B2 (es)
BR (1) BR112018074999A2 (es)
CA (1) CA3031162A1 (es)
ES (1) ES2906310T3 (es)
MX (1) MX2019001631A (es)
MY (1) MY194280A (es)
PT (1) PT3497222T (es)
SG (1) SG11201900930UA (es)
WO (1) WO2018031955A2 (es)

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Publication number Publication date
US20190185826A1 (en) 2019-06-20
JP2022137061A (ja) 2022-09-21
CN109804073B (zh) 2023-05-16
KR20190038612A (ko) 2019-04-08
MY194280A (en) 2022-11-25
EP3497222A2 (en) 2019-06-19
JP2019524134A (ja) 2019-09-05
JP7404068B2 (ja) 2023-12-25
PT3497222T (pt) 2022-02-09
US11091743B2 (en) 2021-08-17
KR102331018B1 (ko) 2021-11-26
AU2017308143B2 (en) 2022-04-07
CN109804073A (zh) 2019-05-24
EP3497222B1 (en) 2021-12-22
WO2018031955A2 (en) 2018-02-15
SG11201900930UA (en) 2019-02-27
RU2019104496A (ru) 2020-09-14
WO2018031955A3 (en) 2018-03-15
AU2017308143A1 (en) 2019-02-07
US20240174987A1 (en) 2024-05-30
ES2906310T3 (es) 2022-04-18
US20210355458A1 (en) 2021-11-18
BR112018074999A2 (pt) 2019-03-12
US11866738B2 (en) 2024-01-09
RU2019104496A3 (es) 2021-04-09
CA3031162A1 (en) 2018-02-15

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