MX2021013124A - Variantes del ácido kaurenoico 13-hidroxilasa (kah) y usos del mismo. - Google Patents

Variantes del ácido kaurenoico 13-hidroxilasa (kah) y usos del mismo.

Info

Publication number
MX2021013124A
MX2021013124A MX2021013124A MX2021013124A MX2021013124A MX 2021013124 A MX2021013124 A MX 2021013124A MX 2021013124 A MX2021013124 A MX 2021013124A MX 2021013124 A MX2021013124 A MX 2021013124A MX 2021013124 A MX2021013124 A MX 2021013124A
Authority
MX
Mexico
Prior art keywords
kah
rebaudioside
steviol glycosides
host cells
variants
Prior art date
Application number
MX2021013124A
Other languages
English (en)
Inventor
Gale Wichmann
Sean Lund
Andrew P Klein
Irina Koryakina
Kyle Hogan
Svetlana Borisova
Original Assignee
Amyris Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amyris Inc filed Critical Amyris Inc
Publication of MX2021013124A publication Critical patent/MX2021013124A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

En el presente documento se proporcionan composiciones, métodos y células hospedadoras modificadas genéticamente para mejorar la producción de glucósidos de esteviol. Las células hospedadoras se modifican genéticamente para contener un ácido nucleico heterólogo que expresa variantes novedosas y optimizadas de Ro.KAH. La célula hospedadora contiene además una o más secuencias de nucleótidos heterólogas que codifican otras enzimas de una vía capaz de producir uno o más glucósidos de esteviol en la célula hospedadora. Las células hospedadoras, las composiciones y los métodos descritos en el presente documento proporcionan una ruta eficaz para la producción heteróloga de glucósidos de esteviol que incluyen rebaudiósido A, rebaudiósido D y rebaudiósido M.
MX2021013124A 2019-05-03 2020-05-01 Variantes del ácido kaurenoico 13-hidroxilasa (kah) y usos del mismo. MX2021013124A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962842810P 2019-05-03 2019-05-03
PCT/US2020/031171 WO2020227148A1 (en) 2019-05-03 2020-05-01 Kaurenoic acid 13-hydroxylase (kah) variants and uses thereof

Publications (1)

Publication Number Publication Date
MX2021013124A true MX2021013124A (es) 2022-01-24

Family

ID=73051309

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021013124A MX2021013124A (es) 2019-05-03 2020-05-01 Variantes del ácido kaurenoico 13-hidroxilasa (kah) y usos del mismo.

Country Status (11)

Country Link
US (1) US20220282228A1 (es)
EP (1) EP3962931A4 (es)
JP (1) JP2022530986A (es)
KR (1) KR20220005021A (es)
CN (1) CN113906045A (es)
AU (1) AU2020268897A1 (es)
BR (1) BR112021022010A2 (es)
CA (1) CA3134729A1 (es)
MX (1) MX2021013124A (es)
SG (1) SG11202111698SA (es)
WO (1) WO2020227148A1 (es)

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102114493B1 (ko) * 2011-08-08 2020-05-26 에볼바 에스아 스테비올 글리코시드의 재조합 생산
CN104928263B (zh) * 2014-03-20 2018-03-23 中国科学院上海生命科学研究院 一种新的贝壳烯酸‑13α‑羟化酶、其编码基因及其应用
CA2960693A1 (en) * 2014-09-09 2016-03-17 Evolva Sa Production of steviol glycosides in recombinant hosts
CA2973674A1 (en) * 2015-01-30 2016-08-04 Evolva Sa Production of steviol glycosides in recombinant hosts
CA2987630A1 (en) * 2015-05-29 2016-12-08 Cargill, Incorporated Fermentation methods for producing steviol glycosides with multi-phase feeding
US10760085B2 (en) * 2015-10-05 2020-09-01 Dsm Ip Assets B.V. Kaurenoic acid hydroxylases
WO2018211032A1 (en) * 2017-05-17 2018-11-22 Evolva Sa Production of steviol glycosides in recombinant hosts
CN113260699A (zh) * 2018-10-15 2021-08-13 阿迈瑞斯公司 用于高效生成瑞鲍迪苷的甜叶菊异贝壳杉烯酸羟化酶变体

Also Published As

Publication number Publication date
EP3962931A4 (en) 2023-06-14
WO2020227148A9 (en) 2021-07-29
KR20220005021A (ko) 2022-01-12
US20220282228A1 (en) 2022-09-08
AU2020268897A1 (en) 2021-11-18
SG11202111698SA (en) 2021-11-29
CN113906045A (zh) 2022-01-07
BR112021022010A2 (pt) 2021-12-21
CA3134729A1 (en) 2020-11-12
WO2020227148A1 (en) 2020-11-12
EP3962931A1 (en) 2022-03-09
JP2022530986A (ja) 2022-07-05

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