MX2021005861A - Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol. - Google Patents

Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol.

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Publication number
MX2021005861A
MX2021005861A MX2021005861A MX2021005861A MX2021005861A MX 2021005861 A MX2021005861 A MX 2021005861A MX 2021005861 A MX2021005861 A MX 2021005861A MX 2021005861 A MX2021005861 A MX 2021005861A MX 2021005861 A MX2021005861 A MX 2021005861A
Authority
MX
Mexico
Prior art keywords
enzymes
methods
present
rebaudioside
polypeptides
Prior art date
Application number
MX2021005861A
Other languages
English (en)
Inventor
Nikki Dellas
Courtney Dianne Moffett
Jonathan Vroom
Stephanie Sue Galanie
Jack Liang
Joyce Liu
David Entwistle
Original Assignee
Codexis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis Inc filed Critical Codexis Inc
Publication of MX2021005861A publication Critical patent/MX2021005861A/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • C12N9/1062Sucrose synthase (2.4.1.13)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/18Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/44Preparation of O-glycosides, e.g. glucosides
    • C12P19/56Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y204/00Glycosyltransferases (2.4)
    • C12Y204/01Hexosyltransferases (2.4.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y204/00Glycosyltransferases (2.4)
    • C12Y204/01Hexosyltransferases (2.4.1)
    • C12Y204/01013Sucrose synthase (2.4.1.13)

Abstract

La presente invención proporciona enzimas de glucosiltransferasa (GT) modificadas con ingeniería genética, polipéptidos que tienen actividad de GT y polinucleótidos que codifican estas enzimas, así como vectores y células hospederas que comprenden estos polinucleótidos y polipéptidos; la presente invención proporciona enzimas de sacarosa sintasa (SuS) modificadas con ingeniería genética, polipéptidos que tienen actividad de SuS y polinucleótidos que codifican estas enzimas, así como vectores y células hospederas que comprenden estos polinucleótidos y polipéptidos; la presente invención también proporciona composiciones que comprenden las enzimas de GT y métodos de uso de las enzimas de GT modificadas con ingeniería genética para elaborar productos con enlaces ß-glucosa; la presente invención proporciona además composiciones y métodos para la producción de rebaudiósidos (por ejemplo, rebaudiósido M, rebaudiósido A, rebaudiósido I y rebaudiósido D); la presente invención también proporciona composiciones que comprenden las enzimas de SuS y métodos para usarlas; también se proporcionan métodos para producir las enzimas de GT y de SuS.
MX2021005861A 2017-02-03 2019-08-02 Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol. MX2021005861A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762454417P 2017-02-03 2017-02-03
US201762479262P 2017-03-30 2017-03-30

Publications (1)

Publication Number Publication Date
MX2021005861A true MX2021005861A (es) 2021-08-11

Family

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Family Applications (4)

Application Number Title Priority Date Filing Date
MX2019009233A MX2019009233A (es) 2017-02-03 2018-02-01 Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol.
MX2022003824A MX2022003824A (es) 2017-02-03 2019-08-02 Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol.
MX2021005862A MX2021005862A (es) 2017-02-03 2019-08-02 Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol.
MX2021005861A MX2021005861A (es) 2017-02-03 2019-08-02 Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol.

Family Applications Before (3)

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MX2019009233A MX2019009233A (es) 2017-02-03 2018-02-01 Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol.
MX2022003824A MX2022003824A (es) 2017-02-03 2019-08-02 Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol.
MX2021005862A MX2021005862A (es) 2017-02-03 2019-08-02 Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol.

Country Status (14)

Country Link
US (4) US20180223264A1 (es)
EP (3) EP3577229A4 (es)
JP (4) JP7248302B2 (es)
KR (2) KR20230162160A (es)
CN (1) CN110914445A (es)
AU (4) AU2018215335B2 (es)
BR (1) BR112019015992A2 (es)
CA (3) CA3175070A1 (es)
IL (2) IL268121B2 (es)
MX (4) MX2019009233A (es)
PH (1) PH12019501698A1 (es)
SG (1) SG11201906480QA (es)
TW (3) TWI797103B (es)
WO (2) WO2018144679A2 (es)

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