MX2021015614A - Enzimas variantes de sacarosa fosforilasa modificadas geneticamente. - Google Patents

Enzimas variantes de sacarosa fosforilasa modificadas geneticamente.

Info

Publication number
MX2021015614A
MX2021015614A MX2021015614A MX2021015614A MX2021015614A MX 2021015614 A MX2021015614 A MX 2021015614A MX 2021015614 A MX2021015614 A MX 2021015614A MX 2021015614 A MX2021015614 A MX 2021015614A MX 2021015614 A MX2021015614 A MX 2021015614A
Authority
MX
Mexico
Prior art keywords
enzymes
sucrose phosphorylase
engineered sucrose
variant enzymes
present
Prior art date
Application number
MX2021015614A
Other languages
English (en)
Inventor
Jonathan Vroom
Santhosh Sivaramakrishnan
Nandhitha Subramanian
Original Assignee
Codexis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis Inc filed Critical Codexis Inc
Publication of MX2021015614A publication Critical patent/MX2021015614A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/45—Transferases (2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/38—Nucleosides
    • C12P19/40—Nucleosides having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same ring, e.g. purine nucleosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01007—Sucrose phosphorylase (2.4.1.7)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/101—Plasmid DNA for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención proporciona enzimas sacarosa fosforilasa (SP) modificadas genéticamente, polipéptidos que tienen actividad SP y polinucleótidos que codifican estas enzimas, así como vectores y células hospederas que comprenden estos polinucleótidos y polipéptidos; también se proporcionan métodos para producir enzimas SP; la presente invención proporciona además composiciones que comprenden las enzimas SP y métodos para usar las enzimas SP modificadas genéticamente; la presente invención encuentra un uso particular en la producción de compuestos farmacéuticos.
MX2021015614A 2019-07-02 2020-06-30 Enzimas variantes de sacarosa fosforilasa modificadas geneticamente. MX2021015614A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962869670P 2019-07-02 2019-07-02
PCT/US2020/040250 WO2021003132A1 (en) 2019-07-02 2020-06-30 Engineered sucrose phosphorylase variant enzymes

Publications (1)

Publication Number Publication Date
MX2021015614A true MX2021015614A (es) 2022-02-03

Family

ID=74101261

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021015614A MX2021015614A (es) 2019-07-02 2020-06-30 Enzimas variantes de sacarosa fosforilasa modificadas geneticamente.

Country Status (12)

Country Link
US (1) US20220243183A1 (es)
EP (1) EP3993824A4 (es)
JP (1) JP2022538230A (es)
KR (1) KR20220028053A (es)
CN (1) CN114072165A (es)
AU (1) AU2020300454A1 (es)
BR (1) BR112021026326A2 (es)
CA (1) CA3142636A1 (es)
IL (1) IL289509A (es)
MX (1) MX2021015614A (es)
SG (1) SG11202113109YA (es)
WO (1) WO2021003132A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114015667B (zh) * 2021-11-19 2023-08-25 江南大学 一种热稳定性提高的蔗糖磷酸化酶突变体及其应用
CN115044568B (zh) * 2022-06-20 2024-01-23 华熙生物科技股份有限公司 高稳定型的蔗糖磷酸化酶突变体及其应用
CN116987684B (zh) * 2023-08-08 2024-04-26 浙江赞源生物技术有限公司 蔗糖磷酸化酶突变体及其在制备甘油葡糖苷中的应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1632567T3 (da) * 2003-06-06 2011-01-17 Kao Corp Fremgangsmåde til fremstilling af phosphorylase
US7968309B2 (en) * 2003-09-04 2011-06-28 Ezaki Glico Co., Ltd. Method for improving the thermostability of sucrose phosphorylase (SP)
WO2016075219A1 (en) * 2014-11-14 2016-05-19 Universiteit Gent A sucrose phosphorylase for the production of kojibiose
US20190127772A1 (en) * 2016-06-15 2019-05-02 Codexis, Inc. Enzymatic glycosylation of steviol glycosides and other compounds with glucose-1-phosphate
CA3079632A1 (en) * 2017-11-28 2019-06-06 C-Lecta Gmbh Sucrose phosphorylase
US11034948B2 (en) * 2018-07-09 2021-06-15 Codexis, Inc. Engineered phosphopentomutase variant enzymes

Also Published As

Publication number Publication date
US20220243183A1 (en) 2022-08-04
BR112021026326A2 (pt) 2022-02-08
JP2022538230A (ja) 2022-09-01
AU2020300454A1 (en) 2021-12-23
EP3993824A4 (en) 2023-07-05
CN114072165A (zh) 2022-02-18
CA3142636A1 (en) 2021-01-07
IL289509A (en) 2022-03-01
EP3993824A1 (en) 2022-05-11
SG11202113109YA (en) 2021-12-30
KR20220028053A (ko) 2022-03-08
WO2021003132A1 (en) 2021-01-07

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