BR112021026306A2 - Enzima de glicosiltransferase dependente de uridina difosfato - Google Patents

Enzima de glicosiltransferase dependente de uridina difosfato

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Publication number
BR112021026306A2
BR112021026306A2 BR112021026306A BR112021026306A BR112021026306A2 BR 112021026306 A2 BR112021026306 A2 BR 112021026306A2 BR 112021026306 A BR112021026306 A BR 112021026306A BR 112021026306 A BR112021026306 A BR 112021026306A BR 112021026306 A2 BR112021026306 A2 BR 112021026306A2
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BR
Brazil
Prior art keywords
ugt
enzyme
aspects
uridine diphosphate
provides
Prior art date
Application number
BR112021026306A
Other languages
English (en)
Inventor
Eric Donald Jason
Parayil Kumaran Ajikumar
Love Aaron
Toomey Christopher
Nicole S Santos Christine
Original Assignee
Manus Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Manus Bio Inc filed Critical Manus Bio Inc
Publication of BR112021026306A2 publication Critical patent/BR112021026306A2/pt

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/44Preparation of O-glycosides, e.g. glucosides
    • C12P19/56Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y204/00Glycosyltransferases (2.4)
    • C12Y204/01Hexosyltransferases (2.4.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y204/00Glycosyltransferases (2.4)
    • C12Y204/01Hexosyltransferases (2.4.1)
    • C12Y204/01262Soyasapogenol glucuronosyltransferase (2.4.1.262)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y204/00Glycosyltransferases (2.4)
    • C12Y204/01Hexosyltransferases (2.4.1)
    • C12Y204/01017Glucuronosyltransferase (2.4.1.17)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

ENZIMA DE GLICOSILTRANSFERASE DEPENDENTE DE URIDINA DIFOSFATO. Em vários aspectos, a presente invenção fornece enzimas glicosiltransferase dependentes de urdina difosfato (UGT) capazes de catalisar a transferência de uma fração de monossacarídeo de um NDP-açúcar para o carbono 3' de uma fração de açúcar de um substrato, tal como um glicano terpenoide, funcionando assim como uma 1-3 UGT. Em outros aspectos, a invenção fornece polinucleotídeos que codificam a 1-3 UGT e células hospedeiras compreendendo os mesmos. Em ainda outros aspectos, a invenção fornece métodos para preparar substratos glicosilados, incluindo glicosídeos de esteviol, usando a enzima e as células hospedeiras desta divulgação.
BR112021026306A 2019-06-25 2020-06-25 Enzima de glicosiltransferase dependente de uridina difosfato BR112021026306A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962866148P 2019-06-25 2019-06-25
PCT/US2020/039648 WO2020264179A1 (en) 2019-06-25 2020-06-25 Uridine diphosphate-dependent glycosyltransferase enzyme

Publications (1)

Publication Number Publication Date
BR112021026306A2 true BR112021026306A2 (pt) 2022-09-06

Family

ID=74062053

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021026306A BR112021026306A2 (pt) 2019-06-25 2020-06-25 Enzima de glicosiltransferase dependente de uridina difosfato

Country Status (11)

Country Link
US (2) US11168309B2 (pt)
EP (1) EP3990628A4 (pt)
JP (1) JP2022530706A (pt)
KR (1) KR20220034793A (pt)
CN (1) CN114174501A (pt)
AU (1) AU2020302789A1 (pt)
BR (1) BR112021026306A2 (pt)
CA (1) CA3144658A1 (pt)
IL (1) IL289202A (pt)
MX (1) MX2022000150A (pt)
WO (1) WO2020264179A1 (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2906310T3 (es) 2016-08-12 2022-04-18 Amyris Inc Glicosiltransferasa dependiente de UDP para la producción de alta eficiencia de rebaudiósidos
EP3765627A4 (en) * 2018-03-12 2021-10-13 Conagen Inc. BIOSYNTHETIC PRODUCTION OF STEVIOL GLYCOSIDES REBAUDIOSIDE J AND REBAUDIOSIDE N
JP7305087B2 (ja) * 2018-07-16 2023-07-10 マナス バイオ インコーポレイテッド 全細胞生体内変換によるステビオールグリコシドの産生
CA3144658A1 (en) * 2019-06-25 2020-12-30 Manus Bio, Inc. Uridine diphosphate-dependent glycosyltransferase enzyme
US20240102068A1 (en) * 2021-01-27 2024-03-28 Korea Advanced Institute Of Science And Technology C-glycosyltransferase variants and use thereof
CN115725528B (zh) * 2021-08-30 2024-01-12 弈柯莱生物科技(集团)股份有限公司 一种糖基转移酶及其应用
KR102628374B1 (ko) * 2021-10-19 2024-01-24 씨제이제일제당 주식회사 신규한 당전이효소 및 이의 용도
WO2023121427A1 (ko) * 2021-12-24 2023-06-29 주식회사 삼양사 당전이 효소 변이체 및 이를 이용한 스테비올 배당체의 제조방법
CN115094074B (zh) * 2022-06-28 2024-02-27 诸城市浩天药业有限公司 双功能udp-糖基转移酶及其应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112012030836B8 (pt) 2010-06-02 2024-02-06 Evolva Nutrition Inc Hospedeiro recombinante que compreende genes recombinantes para produção de esteviol ou glicosídeo de esteviol, método para produzir esteviol, glicosídeo de esteviol ou composição de glicosídeo de esteviol e método para sintetizar esteviol ou glicosídeo de esteviol
WO2013022989A2 (en) 2011-08-08 2013-02-14 Evolva Sa Recombinant production of steviol glycosides
MY190346A (en) 2013-02-06 2022-04-15 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
WO2015011209A1 (en) * 2013-07-23 2015-01-29 Dsm Ip Assets B.V. Diterpene production in yarrowia
CN113549664A (zh) 2014-09-19 2021-10-26 谱赛科有限责任公司 高纯度甜菊醇糖苷
US10463062B2 (en) 2014-11-05 2019-11-05 Manus Bio Inc. Microbial production of steviol glycosides
CA3175070A1 (en) * 2017-02-03 2018-08-09 Codexis, Inc. Engineered glycosyltransferases and steviol glycoside glucosylation methods
JP7305087B2 (ja) * 2018-07-16 2023-07-10 マナス バイオ インコーポレイテッド 全細胞生体内変換によるステビオールグリコシドの産生
CA3144658A1 (en) * 2019-06-25 2020-12-30 Manus Bio, Inc. Uridine diphosphate-dependent glycosyltransferase enzyme

Also Published As

Publication number Publication date
EP3990628A4 (en) 2023-08-02
US11168309B2 (en) 2021-11-09
IL289202A (en) 2022-02-01
CN114174501A (zh) 2022-03-11
MX2022000150A (es) 2022-05-20
EP3990628A1 (en) 2022-05-04
US20220090031A1 (en) 2022-03-24
AU2020302789A1 (en) 2022-01-27
CA3144658A1 (en) 2020-12-30
US11788070B2 (en) 2023-10-17
KR20220034793A (ko) 2022-03-18
JP2022530706A (ja) 2022-06-30
US20210009968A1 (en) 2021-01-14
WO2020264179A1 (en) 2020-12-30

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B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]