BR112021026306A2 - Enzima de glicosiltransferase dependente de uridina difosfato - Google Patents
Enzima de glicosiltransferase dependente de uridina difosfatoInfo
- Publication number
- BR112021026306A2 BR112021026306A2 BR112021026306A BR112021026306A BR112021026306A2 BR 112021026306 A2 BR112021026306 A2 BR 112021026306A2 BR 112021026306 A BR112021026306 A BR 112021026306A BR 112021026306 A BR112021026306 A BR 112021026306A BR 112021026306 A2 BR112021026306 A2 BR 112021026306A2
- Authority
- BR
- Brazil
- Prior art keywords
- ugt
- enzyme
- aspects
- uridine diphosphate
- provides
- Prior art date
Links
- 102000051366 Glycosyltransferases Human genes 0.000 title abstract 3
- 108700023372 Glycosyltransferases Proteins 0.000 title abstract 3
- XCCTYIAWTASOJW-XVFCMESISA-N Uridine-5'-Diphosphate Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(=O)OP(O)(O)=O)O[C@H]1N1C(=O)NC(=O)C=C1 XCCTYIAWTASOJW-XVFCMESISA-N 0.000 title abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 239000004383 Steviol glycoside Substances 0.000 abstract 1
- 229910052799 carbon Inorganic materials 0.000 abstract 1
- 230000001419 dependent effect Effects 0.000 abstract 1
- 239000001177 diphosphate Substances 0.000 abstract 1
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 abstract 1
- 235000011180 diphosphates Nutrition 0.000 abstract 1
- 150000004676 glycans Chemical class 0.000 abstract 1
- 125000001483 monosaccharide substituent group Chemical group 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
- 229930182488 steviol glycoside Natural products 0.000 abstract 1
- 235000019411 steviol glycoside Nutrition 0.000 abstract 1
- 150000008144 steviol glycosides Chemical class 0.000 abstract 1
- 235000019202 steviosides Nutrition 0.000 abstract 1
- 150000003505 terpenes Chemical class 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01262—Soyasapogenol glucuronosyltransferase (2.4.1.262)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01017—Glucuronosyltransferase (2.4.1.17)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
ENZIMA DE GLICOSILTRANSFERASE DEPENDENTE DE URIDINA DIFOSFATO. Em vários aspectos, a presente invenção fornece enzimas glicosiltransferase dependentes de urdina difosfato (UGT) capazes de catalisar a transferência de uma fração de monossacarídeo de um NDP-açúcar para o carbono 3' de uma fração de açúcar de um substrato, tal como um glicano terpenoide, funcionando assim como uma 1-3 UGT. Em outros aspectos, a invenção fornece polinucleotídeos que codificam a 1-3 UGT e células hospedeiras compreendendo os mesmos. Em ainda outros aspectos, a invenção fornece métodos para preparar substratos glicosilados, incluindo glicosídeos de esteviol, usando a enzima e as células hospedeiras desta divulgação.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962866148P | 2019-06-25 | 2019-06-25 | |
| PCT/US2020/039648 WO2020264179A1 (en) | 2019-06-25 | 2020-06-25 | Uridine diphosphate-dependent glycosyltransferase enzyme |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112021026306A2 true BR112021026306A2 (pt) | 2022-09-06 |
Family
ID=74062053
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112021026306A BR112021026306A2 (pt) | 2019-06-25 | 2020-06-25 | Enzima de glicosiltransferase dependente de uridina difosfato |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US11168309B2 (pt) |
| EP (1) | EP3990628A4 (pt) |
| JP (1) | JP2022530706A (pt) |
| KR (1) | KR20220034793A (pt) |
| CN (1) | CN114174501A (pt) |
| AU (1) | AU2020302789A1 (pt) |
| BR (1) | BR112021026306A2 (pt) |
| CA (1) | CA3144658A1 (pt) |
| IL (1) | IL289202A (pt) |
| MX (1) | MX2022000150A (pt) |
| WO (1) | WO2020264179A1 (pt) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3031162A1 (en) | 2016-08-12 | 2018-02-15 | Amyris, Inc. | Udp-dependent glycosyltransferase for high efficiency production of rebaudiosides |
| EP3765627A4 (en) * | 2018-03-12 | 2021-10-13 | Conagen Inc. | BIOSYNTHETIC PRODUCTION OF STEVIOL GLYCOSIDES REBAUDIOSIDE J AND REBAUDIOSIDE N |
| US11230724B2 (en) * | 2018-07-16 | 2022-01-25 | Manus Bio Inc. | Production of steviol glycosides through whole cell biotransformation of steviol glycoside intermediates |
| BR112021026306A2 (pt) * | 2019-06-25 | 2022-09-06 | Manus Bio Inc | Enzima de glicosiltransferase dependente de uridina difosfato |
| WO2022164226A1 (ko) * | 2021-01-27 | 2022-08-04 | 한국과학기술원 | C-글리코실전이효소 변이체 및 이의 용도 |
| CN115725528B (zh) * | 2021-08-30 | 2024-01-12 | 弈柯莱生物科技(集团)股份有限公司 | 一种糖基转移酶及其应用 |
| KR102628374B1 (ko) * | 2021-10-19 | 2024-01-24 | 씨제이제일제당 주식회사 | 신규한 당전이효소 및 이의 용도 |
| WO2023121426A1 (ko) * | 2021-12-24 | 2023-06-29 | 주식회사 삼양사 | 레바우디오사이드의 생산 |
| CN115094074B (zh) * | 2022-06-28 | 2024-02-27 | 诸城市浩天药业有限公司 | 双功能udp-糖基转移酶及其应用 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR122021005287B1 (pt) * | 2010-06-02 | 2022-02-22 | Evolva, Inc | Hospedeiro recombinante que compreende um gene recombinante para produção de esteviol ou glicosídeo de esteviol |
| WO2013022989A2 (en) * | 2011-08-08 | 2013-02-14 | Evolva Sa | Recombinant production of steviol glycosides |
| WO2014122227A2 (en) | 2013-02-06 | 2014-08-14 | Evolva Sa | Methods for improved production of rebaudioside d and rebaudioside m |
| US10273519B2 (en) * | 2013-07-23 | 2019-04-30 | Dsm Ip Assets B.V. | Diterpene production in Yarrowia |
| BR112017005656A2 (pt) * | 2014-09-19 | 2018-07-31 | Purecircle Sdn Bhd | glicosídeos de esteviol de alta pureza |
| JP6946183B2 (ja) * | 2014-11-05 | 2021-10-06 | マナス バイオシンセシス インコーポレイテッド | ステビオール配糖体の微生物による生成 |
| EP3577229A4 (en) | 2017-02-03 | 2020-12-23 | Codexis, Inc. | MANIPULATED GLYCOSYL TRANSFERASES AND METHOD FOR STEVIOL GLYCOSIDE GLUCOSYLATION |
| US11230724B2 (en) * | 2018-07-16 | 2022-01-25 | Manus Bio Inc. | Production of steviol glycosides through whole cell biotransformation of steviol glycoside intermediates |
| BR112021026306A2 (pt) * | 2019-06-25 | 2022-09-06 | Manus Bio Inc | Enzima de glicosiltransferase dependente de uridina difosfato |
-
2020
- 2020-06-25 BR BR112021026306A patent/BR112021026306A2/pt active Search and Examination
- 2020-06-25 MX MX2022000150A patent/MX2022000150A/es unknown
- 2020-06-25 CN CN202080051996.2A patent/CN114174501A/zh active Pending
- 2020-06-25 KR KR1020227002106A patent/KR20220034793A/ko not_active Application Discontinuation
- 2020-06-25 WO PCT/US2020/039648 patent/WO2020264179A1/en unknown
- 2020-06-25 AU AU2020302789A patent/AU2020302789A1/en active Pending
- 2020-06-25 JP JP2021576711A patent/JP2022530706A/ja active Pending
- 2020-06-25 CA CA3144658A patent/CA3144658A1/en active Pending
- 2020-06-25 EP EP20832927.6A patent/EP3990628A4/en active Pending
- 2020-06-25 US US16/912,374 patent/US11168309B2/en active Active
-
2021
- 2021-10-06 US US17/495,139 patent/US11788070B2/en active Active
- 2021-12-21 IL IL289202A patent/IL289202A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP3990628A1 (en) | 2022-05-04 |
| US20210009968A1 (en) | 2021-01-14 |
| KR20220034793A (ko) | 2022-03-18 |
| US20220090031A1 (en) | 2022-03-24 |
| WO2020264179A1 (en) | 2020-12-30 |
| IL289202A (en) | 2022-02-01 |
| US11788070B2 (en) | 2023-10-17 |
| CN114174501A (zh) | 2022-03-11 |
| MX2022000150A (es) | 2022-05-20 |
| US11168309B2 (en) | 2021-11-09 |
| EP3990628A4 (en) | 2023-08-02 |
| JP2022530706A (ja) | 2022-06-30 |
| CA3144658A1 (en) | 2020-12-30 |
| AU2020302789A1 (en) | 2022-01-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B06A | Patent application procedure suspended [chapter 6.1 patent gazette] |