MX2022000150A - Enzima de glicosiltransferasa dependiente de difosfato de uridina. - Google Patents

Enzima de glicosiltransferasa dependiente de difosfato de uridina.

Info

Publication number
MX2022000150A
MX2022000150A MX2022000150A MX2022000150A MX2022000150A MX 2022000150 A MX2022000150 A MX 2022000150A MX 2022000150 A MX2022000150 A MX 2022000150A MX 2022000150 A MX2022000150 A MX 2022000150A MX 2022000150 A MX2022000150 A MX 2022000150A
Authority
MX
Mexico
Prior art keywords
ugt
uridine diphosphate
aspects
dependent glycosyltransferase
host cells
Prior art date
Application number
MX2022000150A
Other languages
English (en)
Inventor
Ajikumar Parayil Kumaran
Aaron Love
Christine Nicole S Santos
Jason Eric Donald
Christopher Toomey
Original Assignee
Manus Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Manus Bio Inc filed Critical Manus Bio Inc
Publication of MX2022000150A publication Critical patent/MX2022000150A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01262—Soyasapogenol glucuronosyltransferase (2.4.1.262)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01017—Glucuronosyltransferase (2.4.1.17)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

En varios aspectos, la presente invención proporciona enzimas de glicosiltransferasa dependientes de uridina difosfato (UGT) capaces de catalizar la transferencia de un resto de monosacárido desde un azúcar NDP al carbono 3' de un resto de azúcar de un sustrato, tal como un glicano terpenoide, que funciona como una "1-3 UGT". En otros aspectos, la invención proporciona polinucleótidos que codifican la 1-3 UGT, y células huésped que los comprenden. En aún otros aspectos, la invención proporciona métodos para preparar sustratos glucosilados, que incluyen glucósidos de esteviol, usando la enzima y las células huésped de la descripción.
MX2022000150A 2019-06-25 2020-06-25 Enzima de glicosiltransferasa dependiente de difosfato de uridina. MX2022000150A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962866148P 2019-06-25 2019-06-25
PCT/US2020/039648 WO2020264179A1 (en) 2019-06-25 2020-06-25 Uridine diphosphate-dependent glycosyltransferase enzyme

Publications (1)

Publication Number Publication Date
MX2022000150A true MX2022000150A (es) 2022-05-20

Family

ID=74062053

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022000150A MX2022000150A (es) 2019-06-25 2020-06-25 Enzima de glicosiltransferasa dependiente de difosfato de uridina.

Country Status (11)

Country Link
US (2) US11168309B2 (es)
EP (1) EP3990628A4 (es)
JP (1) JP2022530706A (es)
KR (1) KR20220034793A (es)
CN (1) CN114174501A (es)
AU (1) AU2020302789A1 (es)
BR (1) BR112021026306A2 (es)
CA (1) CA3144658A1 (es)
IL (1) IL289202A (es)
MX (1) MX2022000150A (es)
WO (1) WO2020264179A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112018074999A2 (pt) 2016-08-12 2019-03-12 Amyris, Inc. glicosiltransferase dependente de udp para a produção de alta eficiência de rebaudiosídeo
EP3765627A4 (en) * 2018-03-12 2021-10-13 Conagen Inc. BIOSYNTHETIC PRODUCTION OF STEVIOL GLYCOSIDES REBAUDIOSIDE J AND REBAUDIOSIDE N
CA3144658A1 (en) * 2019-06-25 2020-12-30 Manus Bio, Inc. Uridine diphosphate-dependent glycosyltransferase enzyme
US20240102068A1 (en) * 2021-01-27 2024-03-28 Korea Advanced Institute Of Science And Technology C-glycosyltransferase variants and use thereof
CN115725528B (zh) * 2021-08-30 2024-01-12 弈柯莱生物科技(集团)股份有限公司 一种糖基转移酶及其应用
KR102628374B1 (ko) * 2021-10-19 2024-01-24 씨제이제일제당 주식회사 신규한 당전이효소 및 이의 용도
KR20230098495A (ko) * 2021-12-24 2023-07-04 주식회사 삼양사 당전이 효소 변이체 및 이를 이용한 스테비올 배당체의 제조방법
CN115094074B (zh) * 2022-06-28 2024-02-27 诸城市浩天药业有限公司 双功能udp-糖基转移酶及其应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR122021005283B1 (pt) * 2010-06-02 2022-02-22 Evolva, Inc Hospedeiro recombinante que compreende genes recombinantes para produção deesteviol ou glicosídeo de esteviol, método para produzir esteviol, glicosídeo de esteviol ou composição de glicosídeo de esteviol e método para sintetizar esteviol ou glicosídeo de esteviol
KR102114493B1 (ko) 2011-08-08 2020-05-26 에볼바 에스아 스테비올 글리코시드의 재조합 생산
SG11201505597SA (en) * 2013-02-06 2015-08-28 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
EP3024941B1 (en) * 2013-07-23 2019-07-03 DSM IP Assets B.V. Diterpene production in yarrowia
MX2017003666A (es) * 2014-09-19 2018-02-01 Purecircle Sdn Bhd Esteviol glicosidos de alta pureza.
MY182396A (en) * 2014-11-05 2021-01-23 Manus Biosynthesis Inc Microbial production of steviol glycosides
IL268121B2 (en) * 2017-02-03 2024-01-01 Codexis Inc Transgenic glycosyltransferases and steviol glycoside glucosylation methods
BR112021000876A2 (pt) * 2018-07-16 2021-04-13 Manus Bio, Inc. Produção de glicosídeos de esteviol através da biotransformação com células inteiras
CA3144658A1 (en) * 2019-06-25 2020-12-30 Manus Bio, Inc. Uridine diphosphate-dependent glycosyltransferase enzyme

Also Published As

Publication number Publication date
KR20220034793A (ko) 2022-03-18
JP2022530706A (ja) 2022-06-30
EP3990628A1 (en) 2022-05-04
EP3990628A4 (en) 2023-08-02
US20220090031A1 (en) 2022-03-24
US11788070B2 (en) 2023-10-17
WO2020264179A1 (en) 2020-12-30
AU2020302789A1 (en) 2022-01-27
CN114174501A (zh) 2022-03-11
US11168309B2 (en) 2021-11-09
IL289202A (en) 2022-02-01
US20210009968A1 (en) 2021-01-14
BR112021026306A2 (pt) 2022-09-06
CA3144658A1 (en) 2020-12-30

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