BR112019015992A2 - Glicosiltransferase engenheirada, polinucleotídeo engenheirado, vetor, célula hospedeira, métodos para produzir pelo menos uma glicosiltransferase engenheirada, para produzir pelo menos uma variante de sacarose sintase engenheirada, para produzir rebaudiosídeo m, rebaudiosídeo a e/ou rebaudiosídeo i e rebaudiosídeo d e para glicosilação de um substrato, composição, sacarose sintase engenheirada, rebaudiosídeo m, rebaudiosídeo a, rebaudiosídeo i, e, rebaudiosídeo d. - Google Patents
Glicosiltransferase engenheirada, polinucleotídeo engenheirado, vetor, célula hospedeira, métodos para produzir pelo menos uma glicosiltransferase engenheirada, para produzir pelo menos uma variante de sacarose sintase engenheirada, para produzir rebaudiosídeo m, rebaudiosídeo a e/ou rebaudiosídeo i e rebaudiosídeo d e para glicosilação de um substrato, composição, sacarose sintase engenheirada, rebaudiosídeo m, rebaudiosídeo a, rebaudiosídeo i, e, rebaudiosídeo d. Download PDFInfo
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- BR112019015992A2 BR112019015992A2 BR112019015992-4A BR112019015992A BR112019015992A2 BR 112019015992 A2 BR112019015992 A2 BR 112019015992A2 BR 112019015992 A BR112019015992 A BR 112019015992A BR 112019015992 A2 BR112019015992 A2 BR 112019015992A2
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- rebaudioside
- engineered
- glycosyltransferase
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- 238000000034 method Methods 0.000 title abstract 5
- 102000040430 polynucleotide Human genes 0.000 title abstract 5
- 108091033319 polynucleotide Proteins 0.000 title abstract 5
- 239000002157 polynucleotide Substances 0.000 title abstract 5
- 229930188195 rebaudioside Natural products 0.000 title abstract 5
- 239000000203 mixture Substances 0.000 title abstract 4
- RPYRMTHVSUWHSV-CUZJHZIBSA-N rebaudioside D Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O RPYRMTHVSUWHSV-CUZJHZIBSA-N 0.000 title abstract 4
- 239000001512 FEMA 4601 Substances 0.000 title abstract 3
- 108700023372 Glycosyltransferases Proteins 0.000 title abstract 3
- 102000051366 Glycosyltransferases Human genes 0.000 title abstract 3
- HELXLJCILKEWJH-SEAGSNCFSA-N Rebaudioside A Natural products O=C(O[C@H]1[C@@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1)[C@@]1(C)[C@@H]2[C@](C)([C@H]3[C@@]4(CC(=C)[C@@](O[C@H]5[C@H](O[C@H]6[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O6)[C@@H](O[C@H]6[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O6)[C@H](O)[C@@H](CO)O5)(C4)CC3)CC2)CCC1 HELXLJCILKEWJH-SEAGSNCFSA-N 0.000 title abstract 3
- HELXLJCILKEWJH-UHFFFAOYSA-N entered according to Sigma 01432 Natural products C1CC2C3(C)CCCC(C)(C(=O)OC4C(C(O)C(O)C(CO)O4)O)C3CCC2(C2)CC(=C)C21OC(C1OC2C(C(O)C(O)C(CO)O2)O)OC(CO)C(O)C1OC1OC(CO)C(O)C(O)C1O HELXLJCILKEWJH-UHFFFAOYSA-N 0.000 title abstract 3
- HELXLJCILKEWJH-NCGAPWICSA-N rebaudioside A Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HELXLJCILKEWJH-NCGAPWICSA-N 0.000 title abstract 3
- 235000019203 rebaudioside A Nutrition 0.000 title abstract 3
- 239000013598 vector Substances 0.000 title abstract 3
- 108010043934 Sucrose synthase Proteins 0.000 title abstract 2
- GSGVXNMGMKBGQU-PHESRWQRSA-N rebaudioside M Chemical compound C[C@@]12CCC[C@](C)([C@H]1CC[C@@]13CC(=C)[C@@](C1)(CC[C@@H]23)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O[C@@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H]1O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O)C(=O)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O[C@@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H]1O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O GSGVXNMGMKBGQU-PHESRWQRSA-N 0.000 title abstract 2
- 230000013595 glycosylation Effects 0.000 title 1
- 238000006206 glycosylation reaction Methods 0.000 title 1
- QRGRAFPOLJOGRV-UHFFFAOYSA-N rebaudioside F Natural products CC12CCCC(C)(C1CCC34CC(=C)C(CCC23)(C4)OC5OC(CO)C(O)C(OC6OCC(O)C(O)C6O)C5OC7OC(CO)C(O)C(O)C7O)C(=O)OC8OC(CO)C(O)C(O)C8O QRGRAFPOLJOGRV-UHFFFAOYSA-N 0.000 title 1
- HYLAUKAHEAUVFE-AVBZULRRSA-N rebaudioside f Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)CO1)O)O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HYLAUKAHEAUVFE-AVBZULRRSA-N 0.000 title 1
- 239000000758 substrate Substances 0.000 title 1
- 108090000790 Enzymes Proteins 0.000 abstract 8
- 102000004190 Enzymes Human genes 0.000 abstract 8
- 229920001184 polypeptide Polymers 0.000 abstract 4
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 4
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 4
- 238000004519 manufacturing process Methods 0.000 abstract 2
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
- C12N9/1062—Sucrose synthase (2.4.1.13)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01013—Sucrose synthase (2.4.1.13)
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
a presente invenção provê enzimas da glicosiltransferase (gt) engenheiradas, polipeptídeos tendo atividade de gt e polinucleotídeos codificando estas enzimas, assim como vetores e células hospedeiras compreendendo estes polinucleotídeos e polipeptídeos. a presente invenção provê enzimas da sacarose sintase (sus) engenheiradas, polipeptídeos tendo atividade de sus e polinucleotídeos codificando estas enzimas, assim como vetores e células hospedeiras compreendendo estes polinucleotídeos e polipeptídeos. a presente invenção também provê composições compreendendo as enzimas gt e métodos de usar as enzimas gt engenheiradas para fabricar produtos com ligações de ß-glicose. a presente invenção provê ainda composições e métodos para a produção de rebaudiosídeos (por exemplo, rebaudiosídeo m, rebaudiosídeo a, rebaudiosídeo i e rebaudiosídeo d). a presente invenção também provê composições compreendendo as enzimas sus e métodos de usá-las. métodos para produzir as enzimas gt e sus também são providos.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762454417P | 2017-02-03 | 2017-02-03 | |
| US62/454,417 | 2017-02-03 | ||
| US201762479262P | 2017-03-30 | 2017-03-30 | |
| US62/479,262 | 2017-03-30 | ||
| PCT/US2018/016359 WO2018144679A2 (en) | 2017-02-03 | 2018-02-01 | Engineered glycosyltransferases and steviol glycoside glucosylation methods |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112019015992A2 true BR112019015992A2 (pt) | 2020-05-26 |
Family
ID=63038689
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112019015992-4A BR112019015992A2 (pt) | 2017-02-03 | 2018-02-01 | Glicosiltransferase engenheirada, polinucleotídeo engenheirado, vetor, célula hospedeira, métodos para produzir pelo menos uma glicosiltransferase engenheirada, para produzir pelo menos uma variante de sacarose sintase engenheirada, para produzir rebaudiosídeo m, rebaudiosídeo a e/ou rebaudiosídeo i e rebaudiosídeo d e para glicosilação de um substrato, composição, sacarose sintase engenheirada, rebaudiosídeo m, rebaudiosídeo a, rebaudiosídeo i, e, rebaudiosídeo d. |
Country Status (14)
| Country | Link |
|---|---|
| US (4) | US20180223264A1 (pt) |
| EP (3) | EP3882354A3 (pt) |
| JP (4) | JP7248302B2 (pt) |
| KR (2) | KR20230162160A (pt) |
| CN (1) | CN110914445A (pt) |
| AU (4) | AU2018215335B2 (pt) |
| BR (1) | BR112019015992A2 (pt) |
| CA (3) | CA3175070A1 (pt) |
| IL (2) | IL268121B2 (pt) |
| MX (4) | MX2019009233A (pt) |
| PH (1) | PH12019501698A1 (pt) |
| SG (1) | SG11201906480QA (pt) |
| TW (3) | TW202237834A (pt) |
| WO (2) | WO2018144679A2 (pt) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2018144679A2 (en) | 2017-02-03 | 2018-08-09 | Codexis, Inc. | Engineered glycosyltransferases and steviol glycoside glucosylation methods |
| BR112021000876A2 (pt) * | 2018-07-16 | 2021-04-13 | Manus Bio, Inc. | Produção de glicosídeos de esteviol através da biotransformação com células inteiras |
| JP2021532757A (ja) * | 2018-07-30 | 2021-12-02 | コデクシス， インコーポレイテッド | 操作されたグリコシルトランスフェラーゼおよびステビオールグリコシドのグルコシル化方法 |
| CN109609530B (zh) * | 2019-01-28 | 2020-08-04 | 江南大学 | 一种海藻糖合成酶及其在海藻糖生产中的应用 |
| WO2020237226A1 (en) * | 2019-05-23 | 2020-11-26 | Arzeda Corp. | Compositions and methods for producing steviol glycosides |
| WO2020264179A1 (en) * | 2019-06-25 | 2020-12-30 | Manus Bio, Inc. | Uridine diphosphate-dependent glycosyltransferase enzyme |
| CA3164769A1 (en) * | 2019-12-16 | 2021-06-24 | Manus Bio, Inc. | Microbial production of mogrol and mogrosides |
| CN111662942A (zh) * | 2020-05-25 | 2020-09-15 | 安徽金禾实业股份有限公司 | 一种双酶发酵催化生产莱鲍迪苷a的方法 |
| US20230304055A1 (en) | 2020-07-03 | 2023-09-28 | C-Lecta Gmbh | One-pot cell-free glycosylation process |
| EP4232568A1 (en) | 2020-10-22 | 2023-08-30 | DSM IP Assets B.V. | Microorganisms for diterpene production |
| KR102546737B1 (ko) * | 2020-11-25 | 2023-06-22 | 씨제이제일제당 주식회사 | 포도당 전이 스테비아를 포함하는 감미질이 개선된 조성물 |
| CN114958791B (zh) * | 2021-01-08 | 2023-07-28 | 中国科学院华南植物园 | 亚精胺衍生物糖基转移酶LbUGT62及其编码基因和应用 |
| CN112704731B (zh) * | 2021-01-13 | 2023-08-25 | 中国农业科学院哈尔滨兽医研究所（中国动物卫生与流行病学中心哈尔滨分中心） | 蛋白酶s273r抑制细胞焦亡的用途及方法 |
| JP2024505906A (ja) * | 2021-01-27 | 2024-02-08 | コリア アドバンスト インスティテュート オブ サイエンス アンド テクノロジー | Ｃ－グリコシルトランスフェラーゼ変異体およびその用途 |
| WO2022253282A1 (zh) * | 2021-06-01 | 2022-12-08 | 弈柯莱生物科技(上海)股份有限公司 | 一种糖基转移酶及其应用 |
| CN115449514B (zh) * | 2021-06-08 | 2023-09-29 | 弈柯莱生物科技(上海)股份有限公司 | 一种β-1,2-糖基转移酶及其应用 |
| CN113462670B (zh) * | 2021-08-23 | 2023-03-24 | 兴化格林生物制品有限公司 | 一种糖基转移酶突变体及其催化合成莱鲍迪苷m的方法 |
| WO2023044368A1 (en) * | 2021-09-17 | 2023-03-23 | Doublerainbow Biosciences Inc. | Method for producing glycosylated therapeutics by using an immobilized enzyme preparation |
| TW202342764A (zh) * | 2021-10-19 | 2023-11-01 | 南韓商Ｃｊ第一製糖股份有限公司 | 用於製備甜菊糖雙苷d及甜菊糖雙苷m的方法 |
| KR20230098495A (ko) * | 2021-12-24 | 2023-07-04 | 주식회사 삼양사 | 당전이 효소 변이체 및 이를 이용한 스테비올 배당체의 제조방법 |
| CN116426506B (zh) * | 2023-03-10 | 2024-01-30 | 云南师范大学 | 低温活性提高的β-木糖苷酶突变体D259G及其应用 |
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2020
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2021
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2022
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2023
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