BR112017022019A2 - produção de adoçantes não calóricos por meio da utilização de catalisadores de células inteiras modificadas - Google Patents

produção de adoçantes não calóricos por meio da utilização de catalisadores de células inteiras modificadas

Info

Publication number
BR112017022019A2
BR112017022019A2 BR112017022019-9A BR112017022019A BR112017022019A2 BR 112017022019 A2 BR112017022019 A2 BR 112017022019A2 BR 112017022019 A BR112017022019 A BR 112017022019A BR 112017022019 A2 BR112017022019 A2 BR 112017022019A2
Authority
BR
Brazil
Prior art keywords
whole cell
production
caloric sweeteners
cell catalysts
modified whole
Prior art date
Application number
BR112017022019-9A
Other languages
English (en)
Inventor
Mao Guohong
Edward Vick Jacob
LI Shi-yan
Yu Xiaodan
Original Assignee
Conagen Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Conagen Inc. filed Critical Conagen Inc.
Publication of BR112017022019A2 publication Critical patent/BR112017022019A2/pt

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A23L27/36—Terpene glycosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1062—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

são divulgados catalisadores, métodos de produção de células inteiras e métodos de utilização de catalisadores de células inteiras para produzir glicosídeos de esteviol.
BR112017022019-9A 2015-04-14 2016-04-14 produção de adoçantes não calóricos por meio da utilização de catalisadores de células inteiras modificadas BR112017022019A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562147160P 2015-04-14 2015-04-14
US62/147,160 2015-04-14
PCT/US2016/027458 WO2016168413A1 (en) 2015-04-14 2016-04-14 Production of non-caloric sweeteners using engineered whole-cell catalysts

Publications (1)

Publication Number Publication Date
BR112017022019A2 true BR112017022019A2 (pt) 2018-07-31

Family

ID=57126486

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017022019-9A BR112017022019A2 (pt) 2015-04-14 2016-04-14 produção de adoçantes não calóricos por meio da utilização de catalisadores de células inteiras modificadas

Country Status (13)

Country Link
US (1) US10752928B2 (pt)
EP (1) EP3283614B1 (pt)
JP (1) JP6793957B2 (pt)
KR (1) KR102569053B1 (pt)
CN (1) CN107548417B (pt)
AU (1) AU2016250184B2 (pt)
BR (1) BR112017022019A2 (pt)
CA (1) CA2977541A1 (pt)
HK (1) HK1249543A1 (pt)
HU (1) HUE051977T2 (pt)
MX (1) MX2017013210A (pt)
MY (1) MY190212A (pt)
WO (1) WO2016168413A1 (pt)

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US9522929B2 (en) 2014-05-05 2016-12-20 Conagen Inc. Non-caloric sweetener
MX2018006599A (es) * 2015-11-30 2018-09-21 Purecircle Sdn Bhd Proceso para producir glicosidos de esteviol de alta pureza.
WO2017205750A1 (en) * 2016-05-26 2017-11-30 The Regents Of The University Of Michigan Compositions and methods for microbial co-culture
CN106701606B (zh) * 2016-12-19 2021-03-26 广东利世康低碳科技有限公司 一种能降解利用餐厨废弃物的基因工程产朊假丝酵母及其构建方法
EP3577229A4 (en) 2017-02-03 2020-12-23 Codexis, Inc. MANIPULATED GLYCOSYL TRANSFERASES AND METHOD FOR STEVIOL GLYCOSIDE GLUCOSYLATION
WO2019014395A1 (en) * 2017-07-11 2019-01-17 Trait Biosciences, Inc. GENERATION OF WATER-SOLUBLE CANNABINOID COMPOUNDS IN PLANT CELL SUSPENSION YEAST AND CROPS AND MATERIAL COMPOSITIONS
US11905543B2 (en) 2017-07-11 2024-02-20 Trait Biosceinces, Inc. In vivo generation of water-soluble acetylated cannabinoid glycoside compounds in plant cell suspension cultures
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WO2019178541A1 (en) * 2018-03-16 2019-09-19 Purecircle Usa Inc. High-purity steviol glycosides
KR20210027270A (ko) * 2018-06-08 2021-03-10 퓨어써클 유에스에이 잉크. 고순도 스테비올 글리코사이드
CN112805295A (zh) 2018-07-30 2021-05-14 科德克希思公司 工程化糖基转移酶和甜菊醇糖苷葡糖基化方法
CN109234341A (zh) * 2018-09-29 2019-01-18 四川盈嘉合生科技有限公司 全细胞催化合成甜味剂组合物的方法
CN109750072B (zh) * 2019-01-31 2022-04-19 南京工业大学 一种酶法制备莱鲍迪苷e的方法
CN109750071A (zh) * 2019-01-31 2019-05-14 南京工业大学 一种生物催化合成莱鲍迪苷m的方法
CN110564658B (zh) * 2019-09-06 2021-08-17 广西大学 一株大肠杆菌工程菌及其全细胞催化生产甜菊醇的方法
CN110734944B (zh) * 2019-11-11 2023-02-21 中化健康产业发展有限公司 一步法合成莱鲍迪苷m的方法
CN110846363B (zh) * 2019-11-11 2023-02-17 天津大学 一锅法生产莱鲍迪苷d的方法
CN113186141B (zh) * 2021-04-08 2023-01-06 华南理工大学 一种一锅法高效合成莱鲍迪苷m的方法
CN114196696B (zh) * 2021-12-06 2023-10-20 天津大学 一种与特定短肽标签融合能高效催化Reb M生成的重组酶
CN114836447B (zh) * 2022-04-29 2024-05-07 中山大学 一种重组大肠杆菌催化合成莱鲍迪苷d的方法
CN114921434B (zh) * 2022-05-27 2024-02-20 中化健康产业发展有限公司 催化Reb A生产Reb M的重组糖基转移酶

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KR101404728B1 (ko) * 2013-02-28 2014-06-09 씨제이제일제당 (주) 스테비오사이드로부터 리바우디오사이드 a를 제조하는 방법
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CN106103729B (zh) * 2013-11-01 2020-07-07 科纳根公司 甜菊醇糖苷的重组制备
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Also Published As

Publication number Publication date
US20180080055A1 (en) 2018-03-22
JP6793957B2 (ja) 2020-12-02
CN107548417B (zh) 2021-11-09
KR20170135848A (ko) 2017-12-08
AU2016250184B2 (en) 2020-07-30
MY190212A (en) 2022-04-05
EP3283614A1 (en) 2018-02-21
HUE051977T2 (hu) 2021-04-28
EP3283614B1 (en) 2020-07-08
EP3283614A4 (en) 2018-08-29
AU2016250184A1 (en) 2017-09-14
HK1249543A1 (zh) 2018-11-02
JP2018511335A (ja) 2018-04-26
US10752928B2 (en) 2020-08-25
CN107548417A (zh) 2018-01-05
MX2017013210A (es) 2018-03-01
WO2016168413A1 (en) 2016-10-20
KR102569053B1 (ko) 2023-08-21
CA2977541A1 (en) 2016-10-20

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