BR112018071144A2 - produção de glicosídeos de esteviol em hospedeiros recombinantes - Google Patents

produção de glicosídeos de esteviol em hospedeiros recombinantes

Info

Publication number
BR112018071144A2
BR112018071144A2 BR112018071144A BR112018071144A BR112018071144A2 BR 112018071144 A2 BR112018071144 A2 BR 112018071144A2 BR 112018071144 A BR112018071144 A BR 112018071144A BR 112018071144 A BR112018071144 A BR 112018071144A BR 112018071144 A2 BR112018071144 A2 BR 112018071144A2
Authority
BR
Brazil
Prior art keywords
production
steviol glycosides
recombinant hosts
steviol
recombinant
Prior art date
Application number
BR112018071144A
Other languages
English (en)
Other versions
BR112018071144B1 (pt
Inventor
Olsson Kim
Chuang Lim Hallwyl Swee
Douchin Veronique
Original Assignee
Evolva Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva Sa filed Critical Evolva Sa
Publication of BR112018071144A2 publication Critical patent/BR112018071144A2/pt
Publication of BR112018071144B1 publication Critical patent/BR112018071144B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A23L2/60—Sweeteners
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A23L27/36—Terpene glycosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1229—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/04—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C12Y207/04006—Nucleoside-diphosphate kinase (2.7.4.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07009—UTP-glucose-1-phosphate uridylyltransferase (2.7.7.9), i.e. UDP-glucose-pyrophosphorylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/02—Phosphotransferases (phosphomutases) (5.4.2)
    • C12Y504/02002—Phosphoglucomutase (5.4.2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/02—Phosphotransferases (phosphomutases) (5.4.2)
    • C12Y504/02007—Phosphopentomutase (5.4.2.7)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N2030/022—Column chromatography characterised by the kind of separation mechanism
    • G01N2030/027—Liquid chromatography

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Polymers & Plastics (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Non-Alcoholic Beverages (AREA)
  • Seasonings (AREA)

Abstract

a invenção refere-se a microrganismos recombinantes e métodos para a produção de glicosídeos de esteviol e precursores de glicosídeo de esteviol.
BR112018071144-6A 2016-04-13 2017-04-13 Célula hospedeira recombinante que produz um ou mais glicosídeos de esteviol ou uma composição de glicosídeo de esteviol em uma cultura de células e métodos para produzir um ou mais glicosídeos de esteviol ou uma composição de glicosídeo de esteviol, bem como cultura de células e lisado celular da dita célula hospedeira recombinante BR112018071144B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662321850P 2016-04-13 2016-04-13
US62/321,850 2016-04-13
PCT/EP2017/059028 WO2017178632A1 (en) 2016-04-13 2017-04-13 Production of steviol glycosides in recombinant hosts

Publications (2)

Publication Number Publication Date
BR112018071144A2 true BR112018071144A2 (pt) 2019-02-26
BR112018071144B1 BR112018071144B1 (pt) 2023-04-18

Family

ID=58645018

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018071144-6A BR112018071144B1 (pt) 2016-04-13 2017-04-13 Célula hospedeira recombinante que produz um ou mais glicosídeos de esteviol ou uma composição de glicosídeo de esteviol em uma cultura de células e métodos para produzir um ou mais glicosídeos de esteviol ou uma composição de glicosídeo de esteviol, bem como cultura de células e lisado celular da dita célula hospedeira recombinante

Country Status (12)

Country Link
US (2) US10982249B2 (pt)
EP (1) EP3442355A1 (pt)
JP (1) JP2019513392A (pt)
KR (1) KR20180132696A (pt)
CN (1) CN109195457A (pt)
AU (1) AU2017251462B2 (pt)
BR (1) BR112018071144B1 (pt)
CA (1) CA3020671A1 (pt)
MX (1) MX2018012561A (pt)
MY (1) MY198001A (pt)
SG (1) SG11201808856TA (pt)
WO (1) WO2017178632A1 (pt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10982249B2 (en) * 2016-04-13 2021-04-20 Evolva Sa Production of steviol glycosides in recombinant hosts
CN109312378A (zh) 2016-05-16 2019-02-05 埃沃尔瓦公司 在重组宿主中产生甜菊醇糖苷
KR102331018B1 (ko) 2016-08-12 2021-11-26 아미리스 인코퍼레이티드 레바우디오시드의 고효율 생산을 위한 udp 의존성 글리코실트랜스퍼라제
US11396669B2 (en) 2016-11-07 2022-07-26 Evolva Sa Production of steviol glycosides in recombinant hosts
SG11201906480QA (en) 2017-02-03 2019-08-27 Codexis Inc Engineered glycosyltransferases and steviol glycoside glucosylation methods
BR112020009838A2 (pt) * 2017-12-05 2020-11-17 Evolva Sa produção de glicosídeos de esteviol em hospedeiros recombinantes
US11299700B1 (en) 2021-02-19 2022-04-12 Acequia Biotechnology, Llc Bioreactor containers and methods of growing hairy roots using the same
CN113444131B (zh) * 2021-06-24 2022-10-21 鲁东大学 N-乙酰氨基葡萄糖类化合物及其制备方法和应用

Family Cites Families (149)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58149697A (ja) 1982-02-27 1983-09-06 Dainippon Ink & Chem Inc β−１，３グリコシルステビオシドの製造方法
JPS59101408A (ja) 1982-12-02 1984-06-12 Junichi Iwamura 植物生長調整剤
US5198360A (en) 1990-01-19 1993-03-30 Eli Lilly And Company Dna sequence conferring a plaque inhibition phenotype
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US6946587B1 (en) 1990-01-22 2005-09-20 Dekalb Genetics Corporation Method for preparing fertile transgenic corn plants
JPH03277275A (ja) 1990-03-28 1991-12-09 Dainippon Ink & Chem Inc 新規酵素及びその酵素を用いた配糖体の製造方法
US5204253A (en) 1990-05-29 1993-04-20 E. I. Du Pont De Nemours And Company Method and apparatus for introducing biological substances into living cells
US5306862A (en) 1990-10-12 1994-04-26 Amoco Corporation Method and composition for increasing sterol accumulation in higher plants
US5460949A (en) 1990-11-15 1995-10-24 Amoco Corporation Method and composition for increasing the accumulation of squalene and specific sterols in yeast
US5712112A (en) 1992-11-04 1998-01-27 National Science Council Of R.O.C. Gene expression system comprising the promoter region of the alpha-amylase genes
WO1995002683A1 (en) 1993-07-15 1995-01-26 Neose Pharmaceuticals Method of synthesizing saccharide compositions
US7186891B1 (en) 1996-04-12 2007-03-06 University Of Kentucky, Research Foundation Plant cells and plants expressing chimeric isoprenoid synthases
JPH10117776A (ja) 1996-10-22 1998-05-12 Japan Tobacco Inc インディカイネの形質転換方法
JP2001519164A (ja) 1997-10-06 2001-10-23 ザ サーントル ナショナル ド ルシエルシュ シアンティフィク 植物脂肪酸ヒドロキシラーゼ遺伝子
US6255557B1 (en) 1998-03-31 2001-07-03 Her Majesty The Queen In Right Of Canada As Represented By The Ministerof Agriculture And Agri-Food Canada Stevia rebaudiana with altered steviol glycoside composition
CA2630341C (en) 1998-04-14 2011-07-26 Kyowa Hakko Kogyo Co., Ltd. Process for producing isoprenoid compounds by microorganisms
EP0955363A3 (en) 1998-05-06 2004-01-28 F. Hoffmann-La Roche Ag Dna sequences encoding enzymes involved in production of isoprenoids
TWI250210B (en) 1998-05-06 2006-03-01 Dsm Ip Assets Bv An isolated DNA sequence coding for an enzyme involved in the mevalonate pathway or the pathway from isopentenyl pyrophosphate to farnesyl pyrophosphate
DK2305825T3 (en) 1998-07-06 2015-03-02 Dcv Inc A process for the production of vitamins
US6531303B1 (en) 1998-07-06 2003-03-11 Arkion Life Sciences Llc Method of producing geranylgeraniol
AR021636A1 (es) 1998-12-17 2002-07-31 Rubicon Forests Holdings Ltd Materiales y metodos para la modificacion del contenido, la composicion y el metabolismo de los isoprenoides
CA2352488A1 (en) 1998-12-23 2000-06-29 The Samuel Roberts Noble Foundation, Inc. Plant transformation process
ES2253220T3 (es) 1999-04-15 2006-06-01 Calgene Llc Secuencias de acido nucleico de proteinas implicadas en la sintesis de tocoferol.
WO2000063400A2 (en) 1999-04-21 2000-10-26 The Samuel Roberts Noble Foundation Plant transformation process
EP1198575B1 (en) 1999-08-04 2007-09-19 Adelbert Bacher Isoprenoid biosynthesis
WO2001012828A1 (en) 1999-08-18 2001-02-22 Paradigm Genetics, Inc. Methods and apparatus for transformation of monocotyledenous plants using agrobacterium in combination with vacuum filtration
WO2001083769A2 (en) 2000-05-03 2001-11-08 The Salk Institute For Biological Studies Crystallization of 4-diphosphocytidyl-2-c-methylerythritol synthesis
DE10027821A1 (de) 2000-06-05 2001-12-06 Adelbert Bacher Der Mevalonat-unabhängige Isoprenoidbiosyntheseweg
US20030033626A1 (en) 2000-07-31 2003-02-13 Hahn Frederick M. Manipulation of genes of the mevalonate and isoprenoid pathways to create novel traits in transgenic organisms
EP1313867A2 (en) 2000-08-24 2003-05-28 The Scripps Research Institute Stress-regulated genes of plants, transgenic plants containing same, and methods of use
US6818424B2 (en) 2000-09-01 2004-11-16 E. I. Du Pont De Nemours And Company Production of cyclic terpenoids
US6660507B2 (en) 2000-09-01 2003-12-09 E. I. Du Pont De Nemours And Company Genes involved in isoprenoid compound production
US6689601B2 (en) 2000-09-01 2004-02-10 E. I. Du Pont De Nemours And Company High growth methanotropic bacterial strain
CA2422730A1 (en) 2000-09-19 2002-03-28 Microbia, Inc. Modulation of secondary metabolite production by zinc binuclear cluster proteins
AU2001296359B2 (en) 2000-09-29 2006-04-27 Cargill Incorporated Isoprenoid production
US6949362B2 (en) 2000-12-12 2005-09-27 E. I. Du Pont De Nemours And Company Rhodococcus cloning and expression vectors
DK1356037T3 (da) 2001-01-25 2011-06-27 Evolva Ltd Bibliotek med en samling af celler
US20040078846A1 (en) 2002-01-25 2004-04-22 Desouza Mervyn L. Carotenoid biosynthesis
US20050003474A1 (en) 2001-01-26 2005-01-06 Desouza Mervyn L. Carotenoid biosynthesis
DE10201458A1 (de) 2001-04-11 2002-10-17 Adelbert Bacher Intermediate und Enzyme des Mevalonat-unabhängigen Isoprenoidbiosyntheseweg
US7034140B2 (en) 2001-04-24 2006-04-25 E.I. Du Pont De Nemours And Company Genes involved in isoprenoid compound production
EP1392824B1 (en) 2001-06-06 2008-08-20 DSM IP Assets B.V. Improved isoprenoid production
EP1402042A2 (en) 2001-06-22 2004-03-31 Syngenta Participations AG Abiotic stress responsive polynucleotides and polypeptides
NZ513755A (en) 2001-08-24 2001-09-28 Ann Rachel Holmes Protein expression system in yeast comprising a vector encoding a heterologous membrane protein and its application in screening for drugs
US20040072311A1 (en) 2001-08-28 2004-04-15 Dicosimo Deana J. Production of cyclic terpenoids
US20040010815A1 (en) 2001-09-26 2004-01-15 Lange B. Markus Identification and characterization of plant genes
US7172886B2 (en) 2001-12-06 2007-02-06 The Regents Of The University Of California Biosynthesis of isopentenyl pyrophosphate
JP4392483B2 (ja) 2002-08-20 2010-01-06 サントリーホールディングス株式会社 新規糖転移酵素遺伝子
US7351564B2 (en) 2002-09-27 2008-04-01 Dsm Ip Assets B.V. SQS gene
US7098000B2 (en) 2003-06-04 2006-08-29 E. I. Du Pont De Nemoure And Company Method for production of C30-aldehyde carotenoids
JP4643569B2 (ja) 2003-06-12 2011-03-02 ディーエスエム アイピー アセッツ ビー．ブイ． フィードバック耐性メバロン酸キナーゼ
US7569389B2 (en) 2004-09-30 2009-08-04 Ceres, Inc. Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics
US7183089B2 (en) 2004-05-21 2007-02-27 The Regents Of The University Of California Method for enhancing production of isoprenoid compounds
EP2080769A3 (en) 2004-07-02 2010-12-01 Metanomics GmbH Process for the production of fine chemicals
US20060014264A1 (en) 2004-07-13 2006-01-19 Stowers Institute For Medical Research Cre/lox system with lox sites having an extended spacer region
WO2006016395A1 (ja) 2004-08-09 2006-02-16 National University Corporation Tohoku University Ｕｄｐ−グルクロニル基転移酵素およびその遺伝子
KR101169824B1 (ko) 2004-08-19 2012-07-30 디에스엠 아이피 어셋츠 비.브이. 아이소프레노이드의 제조
US7923552B2 (en) 2004-10-18 2011-04-12 SGF Holdings, LLC High yield method of producing pure rebaudioside A
JP4841562B2 (ja) 2004-12-14 2011-12-21 ディーエスエム アイピー アセッツ ビー．ブイ． 改良型メバロネートキナーゼ
EP1871883A1 (en) 2005-03-02 2008-01-02 Metanomics GmbH Process for the production of fine chemicals
WO2006096392A2 (en) 2005-03-04 2006-09-14 Diversa Corporation Enzymes involved in astaxanthin, carotenoid and isoprenoid biosynthetic pathways, genes encoding them and methods of making and using them
WO2006093289A1 (ja) 2005-03-04 2006-09-08 National Institute Of Advanced Industrial Science And Technology Udp-キシロースの製造方法
WO2007022318A2 (en) 2005-08-17 2007-02-22 Cornell Research Foundation Nucleic acids and proteins associated with sucrose accumulation in coffee
EP1916888A4 (en) 2005-08-22 2011-05-18 Co2 Boost Llc DEVICE AND PROCESS FOR GENERATING CO2 USED FOR HARVEST PRODUCTION AND SUBMARINE GARDENING
US8293307B2 (en) 2005-10-11 2012-10-23 Purecircle Sdn Bhd Process for manufacturing a sweetener and use thereof
ES2611887T3 (es) 2005-11-23 2017-05-11 The Coca-Cola Company Composiciones de edulcorante de alta potencia natural con perfil temporal y/o perfil de aroma mejorados
KR100784261B1 (ko) 2006-01-02 2007-12-11 한국과학기술원 탄저균의 포자외막 단백질을 이용한 목적단백질의 미생물표면발현방법
US7927851B2 (en) 2006-03-21 2011-04-19 Vineland Research And Innovation Centre Compositions having ent-kaurenoic acid 13-hydroxylase activity and methods for producing same
AU2007299219A1 (en) 2006-04-05 2008-03-27 Metanomics Gmbh Process for the production of a fine chemical
WO2007136847A2 (en) 2006-05-19 2007-11-29 The Regents Of The University Of California Methods for increasing isoprenoid and isoprenoid precursor production by modulating fatty acid levels
EP2035827B1 (en) 2006-06-19 2012-10-17 Givaudan S.A. Nucleic acid, polypeptide and its use
WO2008008256A2 (en) 2006-07-07 2008-01-17 The Regents Of The University Of California Methods for enhancing production of isoprenoid compounds by host cells
US8257957B2 (en) 2006-09-26 2012-09-04 The Regents Of The University Of California Production of isoprenoids and isoprenoid precursors
US7741119B2 (en) 2006-09-28 2010-06-22 E. I. Du Pont De Nemours And Company Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production
US7629156B2 (en) 2006-09-28 2009-12-08 E.I. Du Pont De Nemours And Company Ethanol production in fermentation of mixed sugars containing xylose
CN101200480B (zh) 2006-12-15 2011-03-30 成都华高药业有限公司 莱鲍迪甙a的提取方法
JP4915917B2 (ja) 2006-12-22 2012-04-11 独立行政法人農業・食品産業技術総合研究機構 ラクト−ｎ−ビオースｉ及びガラクト−ｎ−ビオースの製造方法
US8937168B2 (en) 2007-01-22 2015-01-20 Cargill, Incorporated Method of producing purified rebaudioside A compositions using solvent/antisolvent crystallization
JP2008237110A (ja) 2007-03-27 2008-10-09 Institute Of Physical & Chemical Research ステビオール合成酵素遺伝子及びステビオールの製造方法
US9080187B2 (en) 2007-05-17 2015-07-14 The Board Of Trustees Of The University Of Illinois Methods and compositions for producing solvents
US20080292775A1 (en) 2007-05-22 2008-11-27 The Coca-Cola Company Delivery Systems for Natural High-Potency Sweetener Compositions, Methods for Their Formulation, and Uses
WO2009005704A1 (en) 2007-07-03 2009-01-08 The Regents Of The University Of California Methods of increasing isoprenoid or isoprenoid precursor production
JP2009034080A (ja) 2007-08-03 2009-02-19 Sanei Gen Ffi Inc 新規糖転移酵素、及びそれを利用した配糖体の製造
US7964232B2 (en) 2007-09-17 2011-06-21 Pepsico, Inc. Steviol glycoside isomers
EP2594647A3 (en) 2007-09-21 2013-07-24 BASF Plant Science GmbH Plants with increased yield
ES2901901T3 (es) 2007-12-03 2022-03-24 Dsm Ip Assets Bv Nuevas composiciones nutracéuticas que contienen esteviol y uso de las mismas
CA2710629C (en) 2007-12-27 2016-06-21 Mcneil Nutritionals, Llc Synergistic sweetening compositions
CN102007214A (zh) 2008-02-20 2011-04-06 塞瑞斯公司 赋予植物提高的氮利用效率特性的核苷酸序列和相应的多肽
TWI475963B (zh) 2008-02-25 2015-03-11 Coca Cola Co 甜菊糖苷ａ衍生性產物以及製造彼的方法
EP2285948B1 (en) 2008-03-03 2014-01-08 Joule Unlimited Technologies, Inc. Engineered co2 fixing microorganisms producing carbon-based products of interest
US9005444B2 (en) 2008-05-13 2015-04-14 Cargill, Incorporated Separation of rebaudioside A from stevia glycosides using chromatography
WO2010021001A2 (en) 2008-08-19 2010-02-25 Kaushik Ramakrishnan S Process for preparing sweetener from stevia rebaudiana
KR102061165B1 (ko) 2008-10-03 2019-12-31 모리타 가가쿠 고교 가부시키가이샤 신규 스테비올 배당체
CN101720910B (zh) 2008-10-23 2012-07-25 大闽食品（漳州）有限公司 一种甜菊糖甙的制备方法
US8614085B2 (en) 2009-02-27 2013-12-24 Butamax(Tm) Advanced Biofuels Llc Yeast with increased butanol tolerance involving a multidrug efflux pump gene
US20100297722A1 (en) 2009-05-20 2010-11-25 Board Of Trustees Of Southern Illinois University Transgenic moss producing terpenoids
RU2517602C2 (ru) 2009-06-08 2014-05-27 Йенневайн Биотехнологи Гмбх Синтез нмо
PL2482676T3 (pl) 2009-06-16 2015-03-31 Epc Beijing Natural Products Co Ltd Zmniejszanie lub eliminowanie posmaku w słodziku przy użyciu rebaudiozydu D
WO2011028671A1 (en) 2009-09-04 2011-03-10 Redpoint Bio Corporation Sweetness enhancers including rebaudioside a or d
CN102595932B (zh) 2009-09-22 2014-07-16 红点生物公司 莱苞迪苷c的新多晶型物及其制备和使用方法
ES2457641T3 (es) 2009-10-15 2014-04-28 Purecircle Sdn Bhd Procedimiento para preparar rebaudiósido D de pureza elevada
US8299224B2 (en) 2009-10-15 2012-10-30 Purecircle Sdn Bhd High-purity Rebaudioside D
US8703224B2 (en) 2009-11-04 2014-04-22 Pepsico, Inc. Method to improve water solubility of Rebaudioside D
ES2575790T3 (es) 2009-11-10 2016-07-01 Massachusetts Insitute Of Technology Ingeniería microbiana para la preparación de productos químicos y farmacéuticos a partir de la vía isoprenoide
WO2011062748A1 (en) 2009-11-23 2011-05-26 E.I. Du Pont De Nemours And Company Sucrose transporter genes for increasing plant seed lipids
CN102770539B (zh) 2009-12-10 2016-08-03 明尼苏达大学董事会 Tal效应子介导的dna修饰
AU2010343073A1 (en) 2009-12-28 2012-08-02 The Coca-Cola Company Sweetness enhancers, compositions thereof, and methods for use
KR101244315B1 (ko) 2010-10-19 2013-03-14 이화여자대학교 산학협력단 에탄올―저항성 효모 유전자 및 이의 용도
WO2011140329A1 (en) 2010-05-06 2011-11-10 Ceres, Inc. Transgenic plants having increased biomass
EP2571991A1 (en) 2010-05-20 2013-03-27 Evolva SA Method of producing isoprenoid compounds in yeast
EP2575499A2 (en) 2010-05-31 2013-04-10 Vib Vzw The use of transporters to modulate flavor production by yeast
BR112012030836B8 (pt) 2010-06-02 2024-02-06 Evolva Nutrition Inc Hospedeiro recombinante que compreende genes recombinantes para produção de esteviol ou glicosídeo de esteviol, método para produzir esteviol, glicosídeo de esteviol ou composição de glicosídeo de esteviol e método para sintetizar esteviol ou glicosídeo de esteviol
US20120021111A1 (en) 2010-07-23 2012-01-26 Aexelon Therapeutics, Inc. Natural Low Caloric Sweetener Compositions for Use in Beverages, Foods and Pharmaceuticals, and Their Methods of Manufacture
US20120083593A1 (en) 2010-10-01 2012-04-05 Shanghai Yongyou Bioscience Inc. Separation and Purification of Stevioside and Rebaudioside A
CA2819253A1 (en) 2010-11-30 2012-06-07 Massachusetts Institute Of Technology Microbial production of natural sweeteners, diterpenoid steviol glycosides
KR20130014227A (ko) 2011-07-29 2013-02-07 한국생명공학연구원 신규한 α-글루코실 스테비오사이드 및 이의 제조 방법
JP6126597B2 (ja) 2011-08-08 2017-05-10 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． サフラン化合物の組換え生成のための方法および材料
BR122021015509B1 (pt) 2011-08-08 2022-03-29 Evolva Sa Método para produzir um glicosídeo de esteviol alvo
RU2633644C2 (ru) 2011-11-23 2017-10-16 Эвольва Са Способы ферментативного синтеза могрозидных соединений
CN103159808B (zh) 2011-12-09 2017-03-29 上海泓博智源医药股份有限公司 一种制备天然甜味剂的工艺方法
ES2787899T3 (es) 2011-12-19 2020-10-19 Purecircle Sdn Bhd Métodos para purificar glucósidos de esteviol
DK2806754T3 (en) 2012-01-23 2019-02-18 Dsm Ip Assets Bv Diterpene PREPARATION
JP6251669B2 (ja) 2012-03-16 2017-12-20 サントリーホールディングス株式会社 ステビオール配糖体化酵素およびそれをコードする遺伝子
BR112014028819A2 (pt) 2012-05-22 2017-07-25 Purecircle Sdn Bhd glicosídeos de esteviol de pureza elevada
US9611498B2 (en) 2012-09-29 2017-04-04 Shanghai Institutes For Biological Sciences, Chinese Academy Of Sciences Method for producing stevioside compounds by microorganism
EP2928321A1 (en) 2012-12-05 2015-10-14 Evolva SA Steviol glycoside compositions sensory properties
CA2899276C (en) 2013-02-06 2022-11-01 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
SG10201705993YA (en) 2013-02-11 2017-08-30 Evolva Sa Efficient production of steviol glycosides in recombinant hosts
WO2014191580A1 (en) 2013-05-31 2014-12-04 Dsm Ip Assets B.V. Extracellular diterpene production
DK3004366T3 (da) 2013-05-31 2019-05-20 Dsm Ip Assets Bv Mikroorganismer til diterpenproduktion
KR101559478B1 (ko) * 2013-06-24 2015-10-13 한국생명공학연구원 효소전환법을 이용한 천연 고감미료의 제조방법
EP3885443A1 (en) 2013-07-15 2021-09-29 DSM IP Assets B.V. Diterpene production
US10273519B2 (en) 2013-07-23 2019-04-30 Dsm Ip Assets B.V. Diterpene production in Yarrowia
US20160213039A1 (en) 2013-07-31 2016-07-28 Dsm Ip Assets B.V. Steviol glycosides
US20160185813A1 (en) 2013-07-31 2016-06-30 Dsm Ip Assets B.V. Recovery of steviol glycosides
US9957539B2 (en) 2013-08-02 2018-05-01 Suntory Holdings Limited Method for using hexenol glycosyl transferase
CN103397064B (zh) 2013-08-14 2015-04-15 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙m的方法
EP3039132A2 (en) 2013-08-30 2016-07-06 Evolva SA A method for producing modified resveratrol
WO2015051454A1 (en) 2013-10-07 2015-04-16 Vineland Research And Innovation Centre Compositions and methods for producing steviol and steviol glycosides
EP3114210A2 (en) 2014-03-07 2017-01-11 Evolva SA Methods for recombinant production of saffron compounds
BR112017002783A2 (pt) 2014-08-11 2017-12-19 Evolva Sa produção de glicosídeos de esteviol em hospedeiros recombinantes
MX2017003130A (es) * 2014-09-09 2017-10-24 Evolva Sa Produccion de glucosidos de esteviol en hospederos recombinantes.
KR102536036B1 (ko) 2014-10-03 2023-05-23 코나겐 인크. 비칼로리 감미료 및 합성 방법
CA2973674A1 (en) 2015-01-30 2016-08-04 Evolva Sa Production of steviol glycosides in recombinant hosts
CN115851470A (zh) 2015-03-16 2023-03-28 帝斯曼知识产权资产管理有限公司 Udp-糖基转移酶
CN104845990A (zh) 2015-06-11 2015-08-19 山东大学 拟南芥糖基转移酶基因ugt73c7在提高植物抗病性中的应用
AU2016307066A1 (en) 2015-08-07 2018-02-08 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3387136B1 (en) 2015-12-10 2022-01-05 Evolva SA Production of steviol glycosides in recombinant hosts
US10982249B2 (en) * 2016-04-13 2021-04-20 Evolva Sa Production of steviol glycosides in recombinant hosts

Also Published As

Publication number Publication date
EP3442355A1 (en) 2019-02-20
CA3020671A1 (en) 2017-10-19
KR20180132696A (ko) 2018-12-12
US20190203245A1 (en) 2019-07-04
SG11201808856TA (en) 2018-11-29
AU2017251462B2 (en) 2022-02-03
CN109195457A (zh) 2019-01-11
US20210403970A1 (en) 2021-12-30
MX2018012561A (es) 2019-01-17
BR112018071144B1 (pt) 2023-04-18
MY198001A (en) 2023-07-25
US11821015B2 (en) 2023-11-21
JP2019513392A (ja) 2019-05-30
US10982249B2 (en) 2021-04-20
WO2017178632A1 (en) 2017-10-19
AU2017251462A1 (en) 2018-10-18

Similar Documents

Publication Publication Date Title
BR112018002564A2 (pt) produção de glicosídeos de esteviol em hospedeiros recombinantes
BR112017004712A2 (pt) produção de glicosídeos de esteviol em hospedeiros recombinantes
BR112017002783A2 (pt) produção de glicosídeos de esteviol em hospedeiros recombinantes
BR112018011611A2 (pt) produção de glicosídeos de esteviol em hospedeiros recombinantes
BR112018071144A2 (pt) produção de glicosídeos de esteviol em hospedeiros recombinantes
BR112017016338A2 (pt) produção de glicosídeos de esteviol em hospedeiros recombinantes
BR112018073662A2 (pt) produção de glicosídeos de esteviol em hospedeiros recombinantes
MY190346A (en) Methods for improved production of rebaudioside d and rebaudioside m
MX2017013210A (es) Produccion de endulzantes no caloricos con catalizadores de celulas enteras modificadas.
EP3215629A4 (en) Microbial production of steviol glycosides
AU2016271628B2 (en) Fermentation methods for producing steviol glycosides using high pH and compositions obtained therefrom
EP3468385A4 (en) STEVIOL GLYCOSIDE COMPOSITIONS, PRODUCTION METHODS AND USES
EP3063286A4 (en) Recombinant production of steviol glycosides
SG10201705993YA (en) Efficient production of steviol glycosides in recombinant hosts
BR112018011115A2 (pt) processo para produzir glicosídeos de esteviol de alta pureza
MX2019004368A (es) Produccion biosintetica de glicosidos de esteviol y procesos de los mismos.
EP3337340A4 (en) GLYCOSIDE SOLUTIONS OF STÉVIOL
BR112018000550A2 (pt) composição de glicosídeo de esteviol
BR112018010605A2 (pt) nanoemulsões de bebidas produzidas por processamento de alto cisalhamento
MX2023004432A (es) Composiciones de glicosido de esteviol.
EP3644758A4 (en) HYDROLYSIS OF STEVIOL GLYCOSIDES BY BETA-GLUCOSIDASE
EP3564369A4 (en) STEVIOL GLYCOSIDE HEXOSE TRANSFERASE AND CODING GENE FOR THIS
EP3467115A4 (en) METHOD FOR PRODUCING STEVIOL AND STEVIOL GLYCOSIDE USING AN AOBGL1 HOMOLOG
BR112018000298A2 (pt) composição de glicosídeo de esteviol
MX2018007241A (es) Composiciones de glicosido de esteviol.

Legal Events

Date Code Title Description
B350 Update of information on the portal [chapter 15.35 patent gazette]
B06W Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 13/04/2017, OBSERVADAS AS CONDICOES LEGAIS