BR112016002067A2 - Variantes de enzimas - Google Patents

Variantes de enzimas

Info

Publication number
BR112016002067A2
BR112016002067A2 BR112016002067A BR112016002067A BR112016002067A2 BR 112016002067 A2 BR112016002067 A2 BR 112016002067A2 BR 112016002067 A BR112016002067 A BR 112016002067A BR 112016002067 A BR112016002067 A BR 112016002067A BR 112016002067 A2 BR112016002067 A2 BR 112016002067A2
Authority
BR
Brazil
Prior art keywords
variants
glycosyl hydrolase
methods
enzyme
enzyme variants
Prior art date
Application number
BR112016002067A
Other languages
English (en)
Inventor
Foukaraki Maria
Wilhelmus Hommes Ronaldus
Kaper Thijs
R Kelemen Bradley
Kralj Slavko
E Lantz Suzanne
Nikolaev Igor
Van Der Kley Wilhelmus
Franciscus Thomas Van Lieshout Johannes
Van Stigt Thans Sander
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of BR112016002067A2 publication Critical patent/BR112016002067A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

Abstract

VARIANTES DE UMA ENZIMA GLICOSIL HIDROLASE 61 (GH61) POLINUCLEOTÍDEO, VETOR, CÉLULA HOSPEDEIRA, MÉTODO PARA PRODUZIR AS REFERIDAS VARIANTES, COMPOSIÇÃO E MÉTODOS PARA HIDROLISAR UM SUBSTRATO CELULÓSICO E PARA PRODUÇÃO DE ETANOL. A presente invenção refere-se a variantes da enzima glicosil hidrolase, particularmente, variantes de certas oxidorredutases da família glicosil hidrolase 61. Ácidos nucleicos codificando variantes da glicosil hidrolase, composições incluindo variantes da glicosil hidrolase, métodos de produção das variantes e métodos de uso das variantes também são descritos.
BR112016002067A 2013-07-29 2014-07-24 Variantes de enzimas BR112016002067A2 (pt)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201361859721P 2013-07-29 2013-07-29
US201361859630P 2013-07-29 2013-07-29
US201361859704P 2013-07-29 2013-07-29
US201361859712P 2013-07-29 2013-07-29
US201361859735P 2013-07-29 2013-07-29
US201361859680P 2013-07-29 2013-07-29
US201361859666P 2013-07-29 2013-07-29
PCT/US2014/048075 WO2015017255A1 (en) 2013-07-29 2014-07-24 Variant enzymes

Publications (1)

Publication Number Publication Date
BR112016002067A2 true BR112016002067A2 (pt) 2017-08-29

Family

ID=51299060

Family Applications (3)

Application Number Title Priority Date Filing Date
BR112016002045A BR112016002045A2 (pt) 2013-07-29 2014-07-24 Variantes de enzimas
BR112016002038A BR112016002038A2 (pt) 2013-07-29 2014-07-24 Variantes de enzimas
BR112016002067A BR112016002067A2 (pt) 2013-07-29 2014-07-24 Variantes de enzimas

Family Applications Before (2)

Application Number Title Priority Date Filing Date
BR112016002045A BR112016002045A2 (pt) 2013-07-29 2014-07-24 Variantes de enzimas
BR112016002038A BR112016002038A2 (pt) 2013-07-29 2014-07-24 Variantes de enzimas

Country Status (10)

Country Link
US (4) US10081802B2 (pt)
EP (3) EP3027741B1 (pt)
JP (3) JP2016533739A (pt)
CN (3) CN105916981A (pt)
AU (3) AU2014296574A1 (pt)
BR (3) BR112016002045A2 (pt)
DK (2) DK3027741T3 (pt)
MX (3) MX2016001319A (pt)
WO (3) WO2015017256A1 (pt)
ZA (3) ZA201600631B (pt)

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CN110846332B (zh) * 2019-11-29 2022-05-17 怀化学院 一种果胶酶人工序列及其表达方法和应用
CN111154743B (zh) * 2020-04-01 2021-01-05 黑龙江省农业科学院畜牧兽医分院 突变型纤维素酶及其在玉米青贮中的应用
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CN113913412B (zh) * 2021-10-13 2023-10-03 湖北大学 蛋白酶k突变体及其制备方法
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