EA201991758A1 - Рекомбинантные гликозилтрансферазы и способы гликозилирования стевиоловых гликозидов - Google Patents

Рекомбинантные гликозилтрансферазы и способы гликозилирования стевиоловых гликозидов

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Publication number
EA201991758A1
EA201991758A1 EA201991758A EA201991758A EA201991758A1 EA 201991758 A1 EA201991758 A1 EA 201991758A1 EA 201991758 A EA201991758 A EA 201991758A EA 201991758 A EA201991758 A EA 201991758A EA 201991758 A1 EA201991758 A1 EA 201991758A1
Authority
EA
Eurasian Patent Office
Prior art keywords
enzymes
methods
relates
rebaudioside
polypeptides
Prior art date
Application number
EA201991758A
Other languages
English (en)
Inventor
Джонатан Врум
Стефани Сью Галани
Никки Деллас
Джек Лян
Джойс Лю
Дэвид Энтвистл
Кортни Дайанн Моффетт
Original Assignee
Кодексис, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Кодексис, Инк. filed Critical Кодексис, Инк.
Priority claimed from PCT/US2018/016359 external-priority patent/WO2018144679A2/en
Publication of EA201991758A1 publication Critical patent/EA201991758A1/ru

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01013—Sucrose synthase (2.4.1.13)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Изобретение относится к рекомбинантным ферментам гликозилтрансферазам (ГТ), полипептидам, имеющим активность ГТ, и полинуклеотидам, кодирующим эти ферменты, а также к векторам и клеткам-хозяевам, содержащим такие полинуклеотиды и полипептиды. Настоящее изобретение относится к рекомбинантным ферментам сахарозосинтазам (СС), полипептидам, имеющим активность СС, и полинуклеотидам, кодирующим эти ферменты, а также к векторам и клеткам-хозяевам, содержащим такие полинуклеотиды и полипептиды. Настоящее изобретение также относится к композициям, содержащим ферменты ГТ, и способам применения рекомбинантных ферментов ГТ для получения продуктов с -глюкозными связями. Кроме того, настоящее изобретение относится к композициям и способам получения ребаудиозидов (например, ребаудиозида М, ребаудиозида А, ребаудиозида I и ребаудиозида D). Настоящее изобретение также относится к композициям, содержащим ферменты СС, и способам их применения. Также предложены способы получения ферментов ГТ и СС.
EA201991758A 2017-03-30 2018-02-01 Рекомбинантные гликозилтрансферазы и способы гликозилирования стевиоловых гликозидов EA201991758A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762479262P 2017-03-30 2017-03-30
PCT/US2018/016359 WO2018144679A2 (en) 2017-02-03 2018-02-01 Engineered glycosyltransferases and steviol glycoside glucosylation methods

Publications (1)

Publication Number Publication Date
EA201991758A1 true EA201991758A1 (ru) 2020-03-25

Family

ID=69942813

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201991758A EA201991758A1 (ru) 2017-03-30 2018-02-01 Рекомбинантные гликозилтрансферазы и способы гликозилирования стевиоловых гликозидов

Country Status (1)

Country Link
EA (1) EA201991758A1 (ru)

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