MX367771B - Produccion recombinante de glicosidos de esteviol. - Google Patents

Produccion recombinante de glicosidos de esteviol.

Info

Publication number
MX367771B
MX367771B MX2016005677A MX2016005677A MX367771B MX 367771 B MX367771 B MX 367771B MX 2016005677 A MX2016005677 A MX 2016005677A MX 2016005677 A MX2016005677 A MX 2016005677A MX 367771 B MX367771 B MX 367771B
Authority
MX
Mexico
Prior art keywords
steviol glycosides
recombinant production
steviol
rebaudioside
glycosylation activity
Prior art date
Application number
MX2016005677A
Other languages
English (en)
Other versions
MX2016005677A (es
Inventor
Mao Guohong
Yu Xiaodan
Original Assignee
Conagen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Conagen Inc filed Critical Conagen Inc
Publication of MX2016005677A publication Critical patent/MX2016005677A/es
Publication of MX367771B publication Critical patent/MX367771B/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
    • A21D13/00—Finished or partly finished bakery products
    • A21D13/06—Products with modified nutritive value, e.g. with modified starch content
    • A21D13/062—Products with modified nutritive value, e.g. with modified starch content with modified sugar content; Sugar-free products
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
    • A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
    • A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
    • A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
    • A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
    • A23G3/38—Sucrose-free products
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G4/00—Chewing gum
    • A23G4/06—Chewing gum characterised by the composition containing organic or inorganic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
    • A23L2/52—Adding ingredients
    • A23L2/60—Sweeteners
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A23L27/36—Terpene glycosides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/20—Carbocyclic rings
    • C07H15/24—Condensed ring systems having three or more rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1062—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Polymers & Plastics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Inorganic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Seasonings (AREA)

Abstract

La presente invención se refiere a polipéptidos recombinantes que tienen actividades de UDP-glicosiltransferasa, incluyendo una actividad de glicosilación de 1,2-19-O-glucosa y una actividad de glicosilación de 1,2-13-O-glucosa para sintetizar glucósidos de esteviol; también se provee un método para producir una composición de glicósido de esteviol mediante el uso de tal polipéptido recombinante; también se describen gliccósidos de esteviol referidos como rebaudiósido Z1 y rebaudiosido Z2.
MX2016005677A 2013-11-01 2014-10-03 Produccion recombinante de glicosidos de esteviol. MX367771B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361898571P 2013-11-01 2013-11-01
PCT/US2014/059081 WO2015065650A2 (en) 2013-11-01 2014-10-03 Recombinant production of steviol glycosides

Publications (2)

Publication Number Publication Date
MX2016005677A MX2016005677A (es) 2016-10-28
MX367771B true MX367771B (es) 2019-09-05

Family

ID=53005355

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016005677A MX367771B (es) 2013-11-01 2014-10-03 Produccion recombinante de glicosidos de esteviol.

Country Status (11)

Country Link
US (3) US20160251635A1 (es)
EP (1) EP3063286B1 (es)
JP (1) JP6530760B2 (es)
CN (1) CN106103729B (es)
AU (2) AU2014342939B2 (es)
CA (1) CA2928940C (es)
DK (1) DK3063286T3 (es)
ES (1) ES2709439T3 (es)
MX (1) MX367771B (es)
RU (2) RU2741103C2 (es)
WO (1) WO2015065650A2 (es)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2741103C2 (ru) 2013-11-01 2021-01-22 Конаджен Инк. Рекомбинантное получение стевиол-гликозидов
US9522929B2 (en) 2014-05-05 2016-12-20 Conagen Inc. Non-caloric sweetener
ES2741741T3 (es) 2014-10-03 2020-02-12 Conagen Inc Edulcorantes no calóricos y métodos para sintetizar
CN107249356B (zh) 2014-12-17 2021-08-06 嘉吉公司 用于口服摄入或使用的甜菊醇糖苷化合物、组合物以及用于增强甜菊醇糖苷溶解度的方法
WO2016146711A1 (en) 2015-03-16 2016-09-22 Dsm Ip Assets B.V. Udp-glycosyltransferases
CN113683712B (zh) 2015-04-03 2022-10-21 帝斯曼知识产权资产管理有限公司 甜菊醇糖苷
WO2016168413A1 (en) * 2015-04-14 2016-10-20 Conagen Inc. Production of non-caloric sweeteners using engineered whole-cell catalysts
JP2018521636A (ja) * 2015-05-29 2018-08-09 カーギル・インコーポレイテッド 多段階供給を用いる、ステビオール配糖体を生成するための発酵方法
CN105348337B (zh) * 2015-11-19 2018-07-27 南京诺云生物科技有限公司 一种甜菊苷生物转化制备的甜菊糖衍生物、制备方法及其应用
CN105380224A (zh) * 2015-11-19 2016-03-09 南京诺云生物科技有限公司 一种新型甜菊糖衍生物、制备方法及其应用
US20200308616A1 (en) * 2015-11-30 2020-10-01 Purecircle Sdn Bhd Process for producing high purity steviol glycosides
WO2017198682A1 (en) 2016-05-16 2017-11-23 Evolva Sa Production of steviol glycosides in recombinant hosts
MX2018015534A (es) 2016-06-15 2019-03-14 Codexis Inc Beta-glucosidasas dise?adas y metodos de glucosilacion.
CN110191643B (zh) 2016-10-14 2023-06-06 科纳根公司 甜菊醇糖苷的生物合成生产及其工艺
JP6972123B2 (ja) 2016-10-21 2021-11-24 ペプシコ・インク 酵素法を使用するレバウディオサイドｎを調製するための方法
AU2018215335B2 (en) 2017-02-03 2020-10-01 Tate & Lyle Solutions Usa Llc Engineered glycosyltransferases and steviol glycoside glucosylation methods
EP3592157A4 (en) * 2017-03-06 2021-01-13 Conagen Inc. BIOSYNTHETIC PRODUCTION OF STEVIOL GLYCOSIDE REBAUDIOSIDE D4 FROM REBAUDIOSIDE E
CN111093390A (zh) * 2017-05-15 2020-05-01 谱赛科美国股份有限公司 高纯度甜菊醇糖苷
KR102792406B1 (ko) 2017-06-30 2025-04-08 코나겐 인크. 베타-글루코시다제에 의한 스테비올 글리코시드의 가수분해
CN109423486B (zh) * 2017-08-29 2022-02-25 中国科学院分子植物科学卓越创新中心 新型udp-糖基转移酶及其应用
US11365417B2 (en) 2017-09-12 2022-06-21 Bio Capital Holdings, LLC Biological devices and methods of use thereof to produce steviol glycosides
CN109868265B (zh) * 2017-12-01 2023-04-25 中国科学院分子植物科学卓越创新中心 新型糖基转移酶及其应用
BR112021001661A8 (pt) 2018-07-30 2022-08-09 Codexis Inc Glicosiltransferase modificada, polinucleotídeo modificado, vetor, célula hospedeira, métodos para produzir pelo menos uma glicosiltransferase modificada, para produzir pelo menos uma variante de sacarose sintase, para glicosilação de um substrato, para produzir rebaudiosídeo m, rebaudiosídeo a e/ou rebaudiosídeo i e rebaudiosídeo d, composição, sacarose sintase modificada, pelo menos um rebaudiosídeo, rebaudiosídeo m, rebaudiosídeo a, rebaudiosídeo i, e, rebaudiosídeo d.
WO2020097588A1 (en) 2018-11-09 2020-05-14 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
JP7449586B2 (ja) * 2018-12-12 2024-03-14 コナゲン インコーポレイテッド 変異型ステビオール配糖体の生合成製造
CN113388014B (zh) * 2020-03-12 2023-02-03 中科农福(北京)生物技术有限公司 Os02g0589400蛋白在调控植物化感作用中的应用
KR102628374B1 (ko) * 2021-10-19 2024-01-24 씨제이제일제당 주식회사 신규한 당전이효소 및 이의 용도
WO2023183832A1 (en) 2022-03-23 2023-09-28 Sweegen, Inc. Sweetener comprising rebaudiose m and brazzein

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8277862B2 (en) * 2007-03-14 2012-10-02 Concentrate Manufacturing Company Of Ireland Beverage products having steviol glycosides and at least one acid
CN101701238B (zh) * 2009-10-14 2011-11-16 南京师范大学 一种由甜菊糖苷制备甜茶甙的方法
NZ700097A (en) * 2010-06-02 2015-10-30 Evolva Nutrition Inc Recombinant production of steviol glycosides
US9631215B2 (en) * 2011-08-08 2017-04-25 Evolva Sa Recombinant production of steviol glycosides
US12441753B2 (en) * 2011-12-19 2025-10-14 Purecircle Sdn. Bhd. Methods for purifying steviol glycosides and uses of the same
US20150011660A1 (en) * 2012-03-08 2015-01-08 Purecircle Sdn Bhd High-Purity Rubusoside And Process For Producing Of The Same
RU2499427C1 (ru) * 2012-09-19 2013-11-27 Нина Васильевна Мелишева Композиция подсластителя
RU2741103C2 (ru) 2013-11-01 2021-01-22 Конаджен Инк. Рекомбинантное получение стевиол-гликозидов

Also Published As

Publication number Publication date
RU2741103C2 (ru) 2021-01-22
CN106103729B (zh) 2020-07-07
MX2016005677A (es) 2016-10-28
EP3063286B1 (en) 2018-12-05
JP6530760B2 (ja) 2019-06-12
RU2016120635A (ru) 2017-12-06
US10081826B2 (en) 2018-09-25
RU2016120635A3 (es) 2018-05-31
US20170298404A1 (en) 2017-10-19
JP2017500056A (ja) 2017-01-05
RU2706789C2 (ru) 2019-11-21
DK3063286T3 (en) 2019-03-04
AU2017254856A1 (en) 2017-11-23
US20170081691A1 (en) 2017-03-23
EP3063286A4 (en) 2017-06-21
RU2019135831A (ru) 2020-01-20
WO2015065650A2 (en) 2015-05-07
US20160251635A1 (en) 2016-09-01
AU2014342939B2 (en) 2018-03-22
CN106103729A (zh) 2016-11-09
RU2019135831A3 (es) 2020-06-19
EP3063286A2 (en) 2016-09-07
CA2928940A1 (en) 2015-05-07
ES2709439T3 (es) 2019-04-16
WO2015065650A3 (en) 2015-11-05
CA2928940C (en) 2021-12-14
US10253344B2 (en) 2019-04-09
AU2014342939A1 (en) 2016-05-19

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