MX2016005677A - Produccion recombinante de glicosidos de esteviol. - Google Patents

Produccion recombinante de glicosidos de esteviol.

Info

Publication number
MX2016005677A
MX2016005677A MX2016005677A MX2016005677A MX2016005677A MX 2016005677 A MX2016005677 A MX 2016005677A MX 2016005677 A MX2016005677 A MX 2016005677A MX 2016005677 A MX2016005677 A MX 2016005677A MX 2016005677 A MX2016005677 A MX 2016005677A
Authority
MX
Mexico
Prior art keywords
steviol glycosides
recombinant production
steviol
rebaudioside
glycosylation activity
Prior art date
Application number
MX2016005677A
Other languages
English (en)
Other versions
MX367771B (es
Inventor
Mao Guohong
Yu Xiaodan
Original Assignee
Conagen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Conagen Inc filed Critical Conagen Inc
Publication of MX2016005677A publication Critical patent/MX2016005677A/es
Publication of MX367771B publication Critical patent/MX367771B/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D13/00—Finished or partly finished bakery products
    • A21D13/06—Products with modified nutritive value, e.g. with modified starch content
    • A21D13/062—Products with modified nutritive value, e.g. with modified starch content with modified sugar content; Sugar-free products
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
    • A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
    • A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
    • A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
    • A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
    • A23G3/38—Sucrose-free products
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G4/00—Chewing gum
    • A23G4/06—Chewing gum characterised by the composition containing organic or inorganic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A23L2/60—Sweeteners
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A23L27/36—Terpene glycosides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/20—Carbocyclic rings
    • C07H15/24—Condensed ring systems having three or more rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1062—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)

Abstract

Se proporcionan polipéptidos recombinantes que tienen actividades de UDP-glicosiltransferasa, incluyendo una actividad de glicosilación de 1,2-19-O-glucosa y una actividad de glicosilación de 1,2-13-O-glucosa para sintetizar glucósidos de esteviol; también se provee un método para producir una composición de glicósido de esteviol mediante el uso de tal polipéptido recombinante; también se describen gliccósidos de esteviol referidos como rebaudiósido Z1 y rebaudiosido Z2.
MX2016005677A 2013-11-01 2014-10-03 Produccion recombinante de glicosidos de esteviol. MX367771B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361898571P 2013-11-01 2013-11-01
PCT/US2014/059081 WO2015065650A2 (en) 2013-11-01 2014-10-03 Recombinant production of steviol glycosides

Publications (2)

Publication Number Publication Date
MX2016005677A true MX2016005677A (es) 2016-10-28
MX367771B MX367771B (es) 2019-09-05

Family

ID=53005355

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016005677A MX367771B (es) 2013-11-01 2014-10-03 Produccion recombinante de glicosidos de esteviol.

Country Status (11)

Country Link
US (3) US20160251635A1 (es)
EP (1) EP3063286B1 (es)
JP (1) JP6530760B2 (es)
CN (1) CN106103729B (es)
AU (2) AU2014342939B2 (es)
CA (1) CA2928940C (es)
DK (1) DK3063286T3 (es)
ES (1) ES2709439T3 (es)
MX (1) MX367771B (es)
RU (2) RU2706789C2 (es)
WO (1) WO2015065650A2 (es)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106103729B (zh) 2013-11-01 2020-07-07 科纳根公司 甜菊醇糖苷的重组制备
US9522929B2 (en) 2014-05-05 2016-12-20 Conagen Inc. Non-caloric sweetener
HUE045992T2 (hu) * 2014-10-03 2020-01-28 Conagen Inc Kalóriamentes édesítõszerek és szintézismódszereik
MX2017008043A (es) 2014-12-17 2017-12-11 Cargill Inc Compuestos de glicósido de esteviol, composiciones para ingesta por vía oral o uso oral y metodo para mejorar la solubilidad del glicósido de esteviol.
US10604743B2 (en) * 2015-03-16 2020-03-31 Dsm Ip Assets B.V. UDP-glycosyltransferases
US11344051B2 (en) 2015-04-03 2022-05-31 Dsm Ip Assets B.V. Steviol glycosides
HUE051977T2 (hu) * 2015-04-14 2021-04-28 Conagen Inc Kalóriamentes édesítõk elõállítása mesterséges egész sejtes katalizátorok használatával
BR112017025709B1 (pt) * 2015-05-29 2024-03-05 Cargill, Incorporated Método para produzir glicosídeos de esteviol
CN105380224A (zh) * 2015-11-19 2016-03-09 南京诺云生物科技有限公司 一种新型甜菊糖衍生物、制备方法及其应用
CN105348337B (zh) * 2015-11-19 2018-07-27 南京诺云生物科技有限公司 一种甜菊苷生物转化制备的甜菊糖衍生物、制备方法及其应用
MX2018006599A (es) * 2015-11-30 2018-09-21 Purecircle Sdn Bhd Proceso para producir glicosidos de esteviol de alta pureza.
EP3458599A1 (en) 2016-05-16 2019-03-27 Evolva SA Production of steviol glycosides in recombinant hosts
WO2017218325A1 (en) 2016-06-15 2017-12-21 Codexis, Inc. Engineered beta-glucosidases and glucosylation methods
RU2019114181A (ru) 2016-10-14 2020-11-16 Конаджен Инк. Биосинтетическое получение стевиоловых гликозидов и связанные с этим способы
WO2018072213A1 (zh) * 2016-10-21 2018-04-26 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙n的方法
EP3577229A4 (en) 2017-02-03 2020-12-23 Codexis, Inc. MANIPULATED GLYCOSYL TRANSFERASES AND METHOD FOR STEVIOL GLYCOSIDE GLUCOSYLATION
MX2019010537A (es) * 2017-03-06 2019-11-21 Conagen Inc Produccion biosintetica del rebaudiosido d4 de glucosido de esteviol a partir del rebaudiosido e.
CN111093390A (zh) * 2017-05-15 2020-05-01 谱赛科美国股份有限公司 高纯度甜菊醇糖苷
KR20200024789A (ko) 2017-06-30 2020-03-09 코나겐 인크. 베타-글루코시다제에 의한 스테비올 글리코시드의 가수분해
CN109423486B (zh) * 2017-08-29 2022-02-25 中国科学院分子植物科学卓越创新中心 新型udp-糖基转移酶及其应用
US11365417B2 (en) 2017-09-12 2022-06-21 Bio Capital Holdings, LLC Biological devices and methods of use thereof to produce steviol glycosides
CN109868265B (zh) * 2017-12-01 2023-04-25 中国科学院分子植物科学卓越创新中心 新型糖基转移酶及其应用
WO2020123877A1 (en) * 2018-12-12 2020-06-18 Conagen Inc. Biosynthetic production of variant steviol glycosides
CN113388014B (zh) * 2020-03-12 2023-02-03 中科农福(北京)生物技术有限公司 Os02g0589400蛋白在调控植物化感作用中的应用
KR102628374B1 (ko) * 2021-10-19 2024-01-24 씨제이제일제당 주식회사 신규한 당전이효소 및 이의 용도
WO2023183832A1 (en) 2022-03-23 2023-09-28 Sweegen, Inc. Sweetener comprising rebaudiose m and brazzein

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Publication number Priority date Publication date Assignee Title
US8277862B2 (en) * 2007-03-14 2012-10-02 Concentrate Manufacturing Company Of Ireland Beverage products having steviol glycosides and at least one acid
CN101701238B (zh) * 2009-10-14 2011-11-16 南京师范大学 一种由甜菊糖苷制备甜茶甙的方法
BR122021005304B1 (pt) * 2010-06-02 2022-02-22 Evolva, Inc Hospedeiro recombinante que compreende genes recombinantes para produção de esteviol ou glicosídeo de esteviol, ácido nucleico, método para produzir esteviol, glicosídeo de esteviol ou composição de glicosídeo de esteviol e método para sintetizar esteviol ou glicosídeo de esteviol
CN103732753B (zh) * 2011-08-08 2018-03-30 埃沃尔瓦公司 甜菊醇糖苷类的重组生产
RU2599166C2 (ru) * 2011-12-19 2016-10-10 Дзе Кока-Кола Компани Способы очистки стевиоловых гликозидов и их применение
US20150011660A1 (en) * 2012-03-08 2015-01-08 Purecircle Sdn Bhd High-Purity Rubusoside And Process For Producing Of The Same
RU2499427C1 (ru) * 2012-09-19 2013-11-27 Нина Васильевна Мелишева Композиция подсластителя
CN106103729B (zh) 2013-11-01 2020-07-07 科纳根公司 甜菊醇糖苷的重组制备

Also Published As

Publication number Publication date
MX367771B (es) 2019-09-05
JP6530760B2 (ja) 2019-06-12
US10253344B2 (en) 2019-04-09
US20160251635A1 (en) 2016-09-01
AU2017254856A1 (en) 2017-11-23
RU2019135831A (ru) 2020-01-20
US10081826B2 (en) 2018-09-25
RU2016120635A (ru) 2017-12-06
JP2017500056A (ja) 2017-01-05
CA2928940C (en) 2021-12-14
CN106103729B (zh) 2020-07-07
AU2014342939B2 (en) 2018-03-22
ES2709439T3 (es) 2019-04-16
CN106103729A (zh) 2016-11-09
US20170298404A1 (en) 2017-10-19
WO2015065650A3 (en) 2015-11-05
AU2014342939A1 (en) 2016-05-19
RU2706789C2 (ru) 2019-11-21
EP3063286A2 (en) 2016-09-07
US20170081691A1 (en) 2017-03-23
RU2741103C2 (ru) 2021-01-22
EP3063286B1 (en) 2018-12-05
CA2928940A1 (en) 2015-05-07
DK3063286T3 (en) 2019-03-04
EP3063286A4 (en) 2017-06-21
RU2016120635A3 (es) 2018-05-31
WO2015065650A2 (en) 2015-05-07
RU2019135831A3 (es) 2020-06-19

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