RU2019135831A - Рекомбинантное получение стевиол-гликозидов - Google Patents
Рекомбинантное получение стевиол-гликозидов Download PDFInfo
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- RU2019135831A RU2019135831A RU2019135831A RU2019135831A RU2019135831A RU 2019135831 A RU2019135831 A RU 2019135831A RU 2019135831 A RU2019135831 A RU 2019135831A RU 2019135831 A RU2019135831 A RU 2019135831A RU 2019135831 A RU2019135831 A RU 2019135831A
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- amino acid
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- sucrose synthase
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- 239000004383 Steviol glycoside Substances 0.000 title claims 5
- 229930182488 steviol glycoside Natural products 0.000 title claims 5
- 150000008144 steviol glycosides Chemical class 0.000 title claims 3
- 238000000034 method Methods 0.000 claims 7
- 125000003275 alpha amino acid group Chemical group 0.000 claims 6
- 108010043934 Sucrose synthase Proteins 0.000 claims 4
- 235000019411 steviol glycoside Nutrition 0.000 claims 4
- 235000019202 steviosides Nutrition 0.000 claims 4
- 235000003599 food sweetener Nutrition 0.000 claims 3
- 239000000758 substrate Substances 0.000 claims 3
- 239000003765 sweetening agent Substances 0.000 claims 3
- 241000219194 Arabidopsis Species 0.000 claims 2
- RLLCWNUIHGPAJY-RYBZXKSASA-N Rebaudioside E Natural products O=C(O[C@H]1[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O2)[C@@H](O)[C@@H](O)[C@H](CO)O1)[C@]1(C)[C@@H]2[C@@](C)([C@@H]3[C@@]4(CC(=C)[C@@](O[C@@H]5[C@@H](O[C@@H]6[C@@H](O)[C@H](O)[C@@H](O)[C@H](CO)O6)[C@H](O)[C@@H](O)[C@H](CO)O5)(C4)CC3)CC2)CCC1 RLLCWNUIHGPAJY-RYBZXKSASA-N 0.000 claims 2
- 101710156615 Sucrose synthase 1 Proteins 0.000 claims 2
- HSCJRCZFDFQWRP-JZMIEXBBSA-N UDP-alpha-D-glucose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OP(O)(=O)OP(O)(=O)OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(NC(=O)C=C2)=O)O1 HSCJRCZFDFQWRP-JZMIEXBBSA-N 0.000 claims 2
- HSCJRCZFDFQWRP-UHFFFAOYSA-N Uridindiphosphoglukose Natural products OC1C(O)C(O)C(CO)OC1OP(O)(=O)OP(O)(=O)OCC1C(O)C(O)C(N2C(NC(=O)C=C2)=O)O1 HSCJRCZFDFQWRP-UHFFFAOYSA-N 0.000 claims 2
- 150000001875 compounds Chemical class 0.000 claims 2
- 235000013305 food Nutrition 0.000 claims 2
- 239000008103 glucose Substances 0.000 claims 2
- 229930188195 rebaudioside Natural products 0.000 claims 2
- RLLCWNUIHGPAJY-SFUUMPFESA-N rebaudioside E Chemical group O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O RLLCWNUIHGPAJY-SFUUMPFESA-N 0.000 claims 2
- -1 steviol-glycoside compound Chemical class 0.000 claims 2
- 241000219195 Arabidopsis thaliana Species 0.000 claims 1
- 108700023372 Glycosyltransferases Proteins 0.000 claims 1
- 102000051366 Glycosyltransferases Human genes 0.000 claims 1
- 229930006000 Sucrose Natural products 0.000 claims 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims 1
- 101710156775 Sucrose synthase 3 Proteins 0.000 claims 1
- 108090000992 Transferases Proteins 0.000 claims 1
- 102000004357 Transferases Human genes 0.000 claims 1
- XCCTYIAWTASOJW-UHFFFAOYSA-N UDP-Glc Natural products OC1C(O)C(COP(O)(=O)OP(O)(O)=O)OC1N1C(=O)NC(=O)C=C1 XCCTYIAWTASOJW-UHFFFAOYSA-N 0.000 claims 1
- XCCTYIAWTASOJW-XVFCMESISA-N Uridine-5'-Diphosphate Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(=O)OP(O)(O)=O)O[C@H]1N1C(=O)NC(=O)C=C1 XCCTYIAWTASOJW-XVFCMESISA-N 0.000 claims 1
- 241000219977 Vigna Species 0.000 claims 1
- 235000015173 baked goods and baking mixes Nutrition 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 239000004067 bulking agent Substances 0.000 claims 1
- 235000015218 chewing gum Nutrition 0.000 claims 1
- 229940112822 chewing gum Drugs 0.000 claims 1
- 235000009508 confectionery Nutrition 0.000 claims 1
- 235000014510 cooky Nutrition 0.000 claims 1
- 239000000945 filler Substances 0.000 claims 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims 1
- 239000003112 inhibitor Substances 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 239000005720 sucrose Substances 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D13/00—Finished or partly finished bakery products
- A21D13/06—Products with modified nutritive value, e.g. with modified starch content
- A21D13/062—Products with modified nutritive value, e.g. with modified starch content with modified sugar content; Sugar-free products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
- A23G3/38—Sucrose-free products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G4/00—Chewing gum
- A23G4/06—Chewing gum characterised by the composition containing organic or inorganic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/60—Sweeteners
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/30—Artificial sweetening agents
- A23L27/33—Artificial sweetening agents containing sugars or derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/30—Artificial sweetening agents
- A23L27/33—Artificial sweetening agents containing sugars or derivatives
- A23L27/36—Terpene glycosides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/20—Carbocyclic rings
- C07H15/24—Condensed ring systems having three or more rings
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
- C12N9/1062—Sucrose synthase (2.4.1.13)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
Claims (14)
1. Способ получения композиции стевиол–гликозида или синтез соединения стевиол–гликозида, включающий (А) инкубацию субстрата и УДФ–глюкозы в присутствии рекомбинантной УДФ–гликозилтрансферазы, содержащий аминокислотную последовательность, имеющую по меньшей мере 80% идентичности с SEQ ID No: 6, или (B) инкубацию субстрата, сахарозы, УДФ и УДФ–глюкозы в присутствии рекомбинантной УДФ–гликозилтрансферазы, содержащей аминокислотную последовательность, имеющую по меньшей мере 80% идентичности с SEQ ID No: 6, и сахарозосинтазы в течение времени, достаточного для получения композиции стевиол–гликозида или соединения стевиол–гликозида.
2. Способ по п.1, в котором используют сахарозосинтазу, выбранную из группы, состоящей из сахарозосинтазы 1 Arabidopsis, сахарозосинтазы 3 Arabidopsis, сахарозосинтазы Vigna radiate и их функциональных гомологов.
3. Способ по п.2, в котором указанная сахарозосинтаза имеет по меньшей мере 80% идентичности с аминокислотной последовательностью сахарозосинтазы 1 Arabidopsis thaliana, как указано в SEQ ID No: 9.
4. Способ по п.1, в котором рекомбинантная УДФ–гликозилтрансфераза содержит аминокислотную последовательность, имеющую по меньшей мере 90% идентичности с SEQ ID NO: 6.
5. Способ по п.1, в котором рекомбинантная УДФ–гликозилтрансфераза содержит аминокислотную последовательность, имеющую по меньшей мере 95% идентичности с SEQ ID NO: 6.
6. Способ по п.1, в котором рекомбинантная УДФ–гликозилтрансфераза состоит из аминокислотной последовательности гликозилтрансферазы HV1 согласно SEQ ID NO: 6.
7. Способ по п.1, в котором субстратом является ребаудиозид Е, и глюкозный фрагмент из УДФ–глюкозы переносят и ковалентно связывают с С2'–19–О–глюкозой ребаудиозида Е с получением ребаудиозида Z2.
8. Соединение ребаудиозида Z2, имеющее структуру:
9. Подсластитель, содержащий соединение, имеющее химическую структуру:
10. Подсластитель по п.9, дополнительно содержащий по меньшей мере один компонент из числа наполнителя, объемообразующего вещества и ингибитора слеживания.
11. Пищевой продукт, включающий подслащивающее количество подсластителя по п.9.
12. Пищевой продукт по п.11, выбранный из группы, состоящей из напитков, кондитерских изделий, хлебобулочных изделий, печенья и жевательной резинки.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361898571P | 2013-11-01 | 2013-11-01 | |
| US61/898,571 | 2013-11-01 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2016120635A Division RU2706789C2 (ru) | 2013-11-01 | 2014-10-03 | Рекомбинантное получение стевиол-гликозидов |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| RU2019135831A true RU2019135831A (ru) | 2020-01-20 |
| RU2019135831A3 RU2019135831A3 (ru) | 2020-06-19 |
| RU2741103C2 RU2741103C2 (ru) | 2021-01-22 |
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| RU2016120635A RU2706789C2 (ru) | 2013-11-01 | 2014-10-03 | Рекомбинантное получение стевиол-гликозидов |
| RU2019135831A RU2741103C2 (ru) | 2013-11-01 | 2014-10-03 | Рекомбинантное получение стевиол-гликозидов |
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| RU2016120635A RU2706789C2 (ru) | 2013-11-01 | 2014-10-03 | Рекомбинантное получение стевиол-гликозидов |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US20160251635A1 (ru) |
| EP (1) | EP3063286B1 (ru) |
| JP (1) | JP6530760B2 (ru) |
| CN (1) | CN106103729B (ru) |
| AU (2) | AU2014342939B2 (ru) |
| CA (1) | CA2928940C (ru) |
| DK (1) | DK3063286T3 (ru) |
| ES (1) | ES2709439T3 (ru) |
| MX (1) | MX367771B (ru) |
| RU (2) | RU2706789C2 (ru) |
| WO (1) | WO2015065650A2 (ru) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX367771B (es) | 2013-11-01 | 2019-09-05 | Conagen Inc | Produccion recombinante de glicosidos de esteviol. |
| US9522929B2 (en) | 2014-05-05 | 2016-12-20 | Conagen Inc. | Non-caloric sweetener |
| US9783566B2 (en) | 2014-10-03 | 2017-10-10 | Conagen Inc. | Non-caloric sweeteners and methods for synthesizing |
| AU2015364481B2 (en) | 2014-12-17 | 2020-05-14 | Cargill, Incorporated | Steviol glycoside compounds, compositions for oral ingestion or use, and method for enhancing steviol glycoside solubility |
| EP3862426A3 (en) | 2015-03-16 | 2021-11-17 | DSM IP Assets B.V. | Udp-glycosyltransferases |
| CA2980090A1 (en) | 2015-04-03 | 2016-10-06 | Dsm Ip Assets B.V. | Steviol glycosides |
| MX2017013210A (es) | 2015-04-14 | 2018-03-01 | Conagen Inc | Produccion de endulzantes no caloricos con catalizadores de celulas enteras modificadas. |
| US11225678B2 (en) * | 2015-05-29 | 2022-01-18 | Cargill, Incorporated | Fermentation methods for producing steviol glycosides with multi-phase feeding |
| CN105348337B (zh) * | 2015-11-19 | 2018-07-27 | 南京诺云生物科技有限公司 | 一种甜菊苷生物转化制备的甜菊糖衍生物、制备方法及其应用 |
| CN105380224A (zh) * | 2015-11-19 | 2016-03-09 | 南京诺云生物科技有限公司 | 一种新型甜菊糖衍生物、制备方法及其应用 |
| MX2018006599A (es) * | 2015-11-30 | 2018-09-21 | Purecircle Sdn Bhd | Proceso para producir glicosidos de esteviol de alta pureza. |
| CN109312378A (zh) | 2016-05-16 | 2019-02-05 | 埃沃尔瓦公司 | 在重组宿主中产生甜菊醇糖苷 |
| EA201990035A1 (ru) | 2016-06-15 | 2019-06-28 | Кодексис, Инк. | Генно-инженерные бета-глюкозидазы и способы глюкозилирования |
| JP7177048B2 (ja) | 2016-10-14 | 2022-11-22 | コナゲン インコーポレイテッド | ステビオール配糖体の生合成製造およびそのためのプロセス |
| RU2737118C2 (ru) | 2016-10-21 | 2020-11-24 | Пепсико, Инк. | Способ получения ребаудиозида n с применением ферментативного способа |
| TWI797103B (zh) * | 2017-02-03 | 2023-04-01 | 美商克迪科思股份有限公司 | 經工程處理之醣基轉移酶及甜菊糖之葡萄糖苷化方法 |
| WO2018164747A1 (en) | 2017-03-06 | 2018-09-13 | Conagen Inc. | Biosynthetic production of steviol glycoside rebaudioside d4 from rebaudioside e |
| US20200157594A1 (en) * | 2017-05-15 | 2020-05-21 | Purecircle Usa Inc. | High-purity steviol glycosides |
| CN110809408A (zh) | 2017-06-30 | 2020-02-18 | 科纳根公司 | 通过β-葡糖苷酶水解甜菊醇糖苷 |
| CN109423486B (zh) * | 2017-08-29 | 2022-02-25 | 中国科学院分子植物科学卓越创新中心 | 新型udp-糖基转移酶及其应用 |
| WO2019055325A2 (en) | 2017-09-12 | 2019-03-21 | Biocapital Holdings, Llc | BIOOGICAL DEVICES AND METHODS OF USING THE SAME TO PRODUCE STEVIOL GLYCOSIDES |
| CN109868265B (zh) * | 2017-12-01 | 2023-04-25 | 中国科学院分子植物科学卓越创新中心 | 新型糖基转移酶及其应用 |
| EP3894577A4 (en) * | 2018-12-12 | 2023-04-26 | Conagen Inc. | BIOSYNTHETIC MANUFACTURE OF VARIANTS OF STEVIOGLYCOSIDES |
| CN113388014B (zh) * | 2020-03-12 | 2023-02-03 | 中科农福(北京)生物技术有限公司 | Os02g0589400蛋白在调控植物化感作用中的应用 |
| KR102628374B1 (ko) * | 2021-10-19 | 2024-01-24 | 씨제이제일제당 주식회사 | 신규한 당전이효소 및 이의 용도 |
| WO2023183832A1 (en) | 2022-03-23 | 2023-09-28 | Sweegen, Inc. | Sweetener comprising rebaudiose m and brazzein |
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| US8277862B2 (en) * | 2007-03-14 | 2012-10-02 | Concentrate Manufacturing Company Of Ireland | Beverage products having steviol glycosides and at least one acid |
| CN101701238B (zh) * | 2009-10-14 | 2011-11-16 | 南京师范大学 | 一种由甜菊糖苷制备甜茶甙的方法 |
| JP6177127B2 (ja) * | 2010-06-02 | 2017-08-09 | エボルバ， インク．Ｅｖｏｌｖａ， Ｉｎｃ． | ステビオールグリコシドの組換え生成 |
| BR122021015509B1 (pt) * | 2011-08-08 | 2022-03-29 | Evolva Sa | Método para produzir um glicosídeo de esteviol alvo |
| BR122020009091B1 (pt) * | 2011-12-19 | 2021-08-17 | The Coca-Cola Company | Método para purificar reb x |
| US20150011660A1 (en) * | 2012-03-08 | 2015-01-08 | Purecircle Sdn Bhd | High-Purity Rubusoside And Process For Producing Of The Same |
| RU2499427C1 (ru) * | 2012-09-19 | 2013-11-27 | Нина Васильевна Мелишева | Композиция подсластителя |
| MX367771B (es) | 2013-11-01 | 2019-09-05 | Conagen Inc | Produccion recombinante de glicosidos de esteviol. |
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