MX2021011770A - Produccion de manool. - Google Patents

Produccion de manool.

Info

Publication number
MX2021011770A
MX2021011770A MX2021011770A MX2021011770A MX2021011770A MX 2021011770 A MX2021011770 A MX 2021011770A MX 2021011770 A MX2021011770 A MX 2021011770A MX 2021011770 A MX2021011770 A MX 2021011770A MX 2021011770 A MX2021011770 A MX 2021011770A
Authority
MX
Mexico
Prior art keywords
cpp
manool
synthase
sclareol
synthases
Prior art date
Application number
MX2021011770A
Other languages
English (en)
Inventor
Michel Schalk
Letizia ROCCI
Laurent Daviet
Escalante Daniel Solis
Original Assignee
Firmenich & Cie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Firmenich & Cie filed Critical Firmenich & Cie
Publication of MX2021011770A publication Critical patent/MX2021011770A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y505/00—Intramolecular lyases (5.5)
    • C12Y505/01—Intramolecular lyases (5.5.1)
    • C12Y505/01012—Copalyl diphosphate synthase (5.5.1.12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/101—Plasmid DNA for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/102—Plasmid DNA for yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

La presente invención se refiere a métodos para producir (+)-manool que comprende: poner en contacto geranilgeranil difosfato con una copalil difosfato sintasa (CPP) para formar un (9S, 10S)-copalil difosfato y poner en contacto el CPP con una enzima de esclareol sintasa para formar (+)-manool y derivados del mismo. También se proporcionan ácidos nucleicos que codifican a CPP sintasas y esclareol sintasas para uso en los métodos. Además se proporcionan vectores de expresión y organismos hospederos no humanos y células que comprenden ácidos nucleicos que codifican una CPP sintasa y una esclareol sintasa como se describe en la presente.
MX2021011770A 2016-12-22 2019-06-06 Produccion de manool. MX2021011770A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP16206349 2016-12-22

Publications (1)

Publication Number Publication Date
MX2021011770A true MX2021011770A (es) 2021-10-22

Family

ID=57629393

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2019006635A MX2019006635A (es) 2016-12-22 2017-12-18 Produccion de manool.
MX2021011770A MX2021011770A (es) 2016-12-22 2019-06-06 Produccion de manool.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2019006635A MX2019006635A (es) 2016-12-22 2017-12-18 Produccion de manool.

Country Status (8)

Country Link
US (2) US10752922B2 (es)
EP (1) EP3559245B1 (es)
JP (1) JP7160811B2 (es)
CN (2) CN110100003B (es)
BR (1) BR112019013014A2 (es)
ES (1) ES2899121T3 (es)
MX (2) MX2019006635A (es)
WO (1) WO2018114839A2 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114630905A (zh) * 2019-07-10 2022-06-14 弗门尼舍有限公司 萜烯化合物受控降解的生物催化方法
WO2023280677A1 (en) 2021-07-06 2023-01-12 Isobionics B.V. Recombinant manufacture of c-20 terpenoid alcohols
CN114150011B (zh) * 2021-11-17 2023-04-18 天津大学 异源合成鼠尾草酸的重组酿酒酵母及构建方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3681853D1 (de) 1985-08-12 1991-11-14 Firmenich & Cie Verfahren zur herstellung von sauerstoff enthaltenden decalinderivaten.
JPH0696571B2 (ja) * 1986-12-24 1994-11-30 ハリマ化成株式会社 ８α，１２−エポキシ−１３，１４，１５，１６−テトラノルラブダンの製造方法
JPH07206655A (ja) * 1994-01-14 1995-08-08 Pola Chem Ind Inc メラニン産生抑制剤及び皮膚外用剤
US7294492B2 (en) 2005-01-07 2007-11-13 International Flavors & Fragrances Inc. Process for the manufacture of spiroketals
WO2009044336A2 (en) * 2007-10-05 2009-04-09 Firmenich Sa Method for producing diterpenes
EP2238256B1 (en) 2008-01-29 2015-10-14 Firmenich S.A. Method for producing sclareol
CN101939430B (zh) * 2008-02-15 2015-05-13 弗门尼舍有限公司 生产香紫苏醇的方法
ES2625861T3 (es) 2011-11-01 2017-07-20 Firmenich Sa Citocromo p450 y su uso para la oxidación enzimática de terpenos
AU2012343263B2 (en) * 2011-11-21 2017-10-26 The University Of British Columbia Diterpene synthases and method for producing diterpenoids
US20150339371A1 (en) 2012-06-28 2015-11-26 Nokia Corporation Method and apparatus for classifying significant places into place categories
US9353385B2 (en) 2012-07-30 2016-05-31 Evolva, Inc. Sclareol and labdenediol diphosphate synthase polypeptides, encoding nucleic acid molecules and uses thereof
DK3004366T3 (da) * 2013-05-31 2019-05-20 Dsm Ip Assets Bv Mikroorganismer til diterpenproduktion
WO2015113570A1 (en) 2014-01-31 2015-08-06 University Of Copenhagen Methods for producing diterpenes
WO2015197075A1 (en) * 2014-06-23 2015-12-30 University Of Copenhagen Methods and materials for production of terpenoids

Also Published As

Publication number Publication date
JP7160811B2 (ja) 2022-10-25
US20210010035A1 (en) 2021-01-14
CN110100003A (zh) 2019-08-06
CN110100003B (zh) 2023-11-17
JP2020513755A (ja) 2020-05-21
US10752922B2 (en) 2020-08-25
CN117604043A (zh) 2024-02-27
WO2018114839A3 (en) 2018-08-30
EP3559245B1 (en) 2021-08-25
ES2899121T3 (es) 2022-03-10
MX2019006635A (es) 2019-08-21
US20190352673A1 (en) 2019-11-21
WO2018114839A2 (en) 2018-06-28
EP3559245A2 (en) 2019-10-30
BR112019013014A2 (pt) 2020-01-14

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