MX2017015827A - Produccion de manool. - Google Patents

Produccion de manool.

Info

Publication number
MX2017015827A
MX2017015827A MX2017015827A MX2017015827A MX2017015827A MX 2017015827 A MX2017015827 A MX 2017015827A MX 2017015827 A MX2017015827 A MX 2017015827A MX 2017015827 A MX2017015827 A MX 2017015827A MX 2017015827 A MX2017015827 A MX 2017015827A
Authority
MX
Mexico
Prior art keywords
manool
cpp
synthase
seq
production
Prior art date
Application number
MX2017015827A
Other languages
English (en)
Inventor
Schalk Michel
Rocci Letizia
Original Assignee
Firmenich & Cie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Firmenich & Cie filed Critical Firmenich & Cie
Publication of MX2017015827A publication Critical patent/MX2017015827A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/002—Preparation of hydrocarbons or halogenated hydrocarbons cyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y505/00—Intramolecular lyases (5.5)
    • C12Y505/01—Intramolecular lyases (5.5.1)
    • C12Y505/01012—Copalyl diphosphate synthase (5.5.1.12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y505/00—Intramolecular lyases (5.5)
    • C12Y505/01—Intramolecular lyases (5.5.1)
    • C12Y505/01014—Syn-copalyl-diphosphate synthase (5.5.1.14)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

La presente invención proporciona aquí métodos para producir (+)-manool que comprenden: poner en contacto el difosfato de geranilgeranilo con una difosfato de copalilo (CPP) sintasa para formar un difosfato de (9S,10S)-copalilo en donde la CPP sintasa comprende una secuencia de aminoácidos que tiene al menos 90 %, 95 %, 98 %, 99 y 100 % de identidad de la secuencia con un polipéptido seleccionado del grupo que consiste de la SEC ID NO:1 y la SEC ID NO:2; y poner en contacto el CPP con una enzima de esclareol sintasa para formar el (+)-manool.
MX2017015827A 2015-06-30 2016-06-30 Produccion de manool. MX2017015827A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562187236P 2015-06-30 2015-06-30
GBGB1601249.4A GB201601249D0 (en) 2016-01-22 2016-01-22 Production of manool
PCT/EP2016/065448 WO2017001641A1 (en) 2015-06-30 2016-06-30 Production of manool

Publications (1)

Publication Number Publication Date
MX2017015827A true MX2017015827A (es) 2018-04-10

Family

ID=55534814

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017015827A MX2017015827A (es) 2015-06-30 2016-06-30 Produccion de manool.

Country Status (9)

Country Link
US (1) US10385361B2 (es)
EP (1) EP3317418B1 (es)
JP (1) JP6856553B2 (es)
CN (1) CN107889506B (es)
CA (1) CA2987487A1 (es)
ES (1) ES2933968T3 (es)
GB (1) GB201601249D0 (es)
MX (1) MX2017015827A (es)
WO (1) WO2017001641A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7406511B2 (ja) * 2018-07-10 2023-12-27 フイルメニツヒ ソシエテ アノニム テルペン化合物の生体触媒的な製造方法
WO2021005097A1 (en) * 2019-07-10 2021-01-14 Firmenich Sa Biocatalytic method for the controlled degradation of terpene compounds

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3681853D1 (de) 1985-08-12 1991-11-14 Firmenich & Cie Verfahren zur herstellung von sauerstoff enthaltenden decalinderivaten.
CN101319220B (zh) * 2007-06-05 2010-08-25 中国中医科学院中药研究所 一种丹参二萜合酶基因及其编码产物与应用
CN102016051B (zh) * 2008-01-29 2014-05-28 弗门尼舍有限公司 生产香紫苏醇的方法
EP2366782A1 (en) * 2010-03-09 2011-09-21 Sandoz AG Polypeptide having diterpene synthase activity
US10000773B2 (en) 2011-11-01 2018-06-19 Firmenich Sa Cytochrome P450 and use thereof for the enzymatic oxidation of terpenes
EP2880159A1 (en) 2012-07-30 2015-06-10 Allylix, Inc. Sclareol and labdenediol diphosphate synthase polypeptides encoding nucleic acid molecules and uses thereof

Also Published As

Publication number Publication date
CA2987487A1 (en) 2017-01-05
WO2017001641A1 (en) 2017-01-05
JP2018527892A (ja) 2018-09-27
JP6856553B2 (ja) 2021-04-07
BR112017028183A2 (pt) 2018-08-28
GB201601249D0 (en) 2016-03-09
ES2933968T3 (es) 2023-02-15
CN107889506B (zh) 2021-09-14
EP3317418A1 (en) 2018-05-09
US20190002925A1 (en) 2019-01-03
CN107889506A (zh) 2018-04-06
US10385361B2 (en) 2019-08-20
EP3317418B1 (en) 2022-11-30

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