MX2019004013A - Aislamiento temprano de celulas despues de la transfeccion (epic) para la produccion de productos biologicos. - Google Patents

Aislamiento temprano de celulas despues de la transfeccion (epic) para la produccion de productos biologicos.

Info

Publication number
MX2019004013A
MX2019004013A MX2019004013A MX2019004013A MX2019004013A MX 2019004013 A MX2019004013 A MX 2019004013A MX 2019004013 A MX2019004013 A MX 2019004013A MX 2019004013 A MX2019004013 A MX 2019004013A MX 2019004013 A MX2019004013 A MX 2019004013A
Authority
MX
Mexico
Prior art keywords
cells
epic
sorting
early post
polypeptide
Prior art date
Application number
MX2019004013A
Other languages
English (en)
Inventor
Demaria Christine
R Cairns Victor
Vitko Jason
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genzyme Corp filed Critical Genzyme Corp
Publication of MX2019004013A publication Critical patent/MX2019004013A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70592—CD52
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70596—Molecules with a "CD"-designation not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0081—Purging biological preparations of unwanted cells
    • C12N5/0087—Purging against subsets of blood cells, e.g. purging alloreactive T cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Epoxy Compounds (AREA)

Abstract

En la presente invención se proporcionan métodos para seleccionar una población de células que expresan un polipéptido blanco. En algunos aspectos, la descripción proporciona métodos para la clasificación y selección de poblaciones de células huésped transfectadas basándose en su expresión temprana de un polipéptido seleccionable. En ciertas modalidades, la clasificación se realiza utilizando clasificación celular activada por fluorescencia o clasificación celular activada por magnetismo basándose en el polipéptido seleccionable. Tales métodos de selección se pueden utilizar adicionalmente para generar poblaciones clonales de células productoras, p. ej., para la fabricación a gran escala de un polipéptido blanco de interés.
MX2019004013A 2016-10-07 2017-10-06 Aislamiento temprano de celulas despues de la transfeccion (epic) para la produccion de productos biologicos. MX2019004013A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662405392P 2016-10-07 2016-10-07
PCT/US2017/055583 WO2018067964A1 (en) 2016-10-07 2017-10-06 Early post-transfection isolation of cells (epic) for biologics production

Publications (1)

Publication Number Publication Date
MX2019004013A true MX2019004013A (es) 2019-08-14

Family

ID=60302441

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2019004013A MX2019004013A (es) 2016-10-07 2017-10-06 Aislamiento temprano de celulas despues de la transfeccion (epic) para la produccion de productos biologicos.

Country Status (9)

Country Link
US (3) US20180119192A1 (es)
EP (1) EP3523440A1 (es)
CN (1) CN110050065B (es)
AU (2) AU2017341028B2 (es)
CA (1) CA3039598A1 (es)
MX (1) MX2019004013A (es)
SG (2) SG11201903018WA (es)
TW (2) TWI795373B (es)
WO (1) WO2018067964A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108368504B (zh) 2015-10-09 2022-07-19 建新公司 细胞的早期转染后分离(epic)用于生物制品产生
CA3039598A1 (en) 2016-10-07 2018-04-12 Genzyme Corporation Early post-transfection isolation of cells (epic) for biologics production

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Also Published As

Publication number Publication date
WO2018067964A1 (en) 2018-04-12
TW201827598A (zh) 2018-08-01
US20180119192A1 (en) 2018-05-03
AU2017341028A1 (en) 2019-05-23
US20200299743A1 (en) 2020-09-24
SG10202103545PA (en) 2021-05-28
TWI795373B (zh) 2023-03-11
US20240026410A1 (en) 2024-01-25
CN110050065B (zh) 2024-05-07
SG11201903018WA (en) 2019-05-30
EP3523440A1 (en) 2019-08-14
AU2017341028B2 (en) 2023-12-21
TW202346598A (zh) 2023-12-01
CA3039598A1 (en) 2018-04-12
CN110050065A (zh) 2019-07-23
AU2024201853A1 (en) 2024-04-11
US11685943B2 (en) 2023-06-27

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