MX2016001396A - Seleccion novedosa de vectores y metodos de seleccion de celulas hospederas eucarioticas. - Google Patents
Seleccion novedosa de vectores y metodos de seleccion de celulas hospederas eucarioticas.Info
- Publication number
- MX2016001396A MX2016001396A MX2016001396A MX2016001396A MX2016001396A MX 2016001396 A MX2016001396 A MX 2016001396A MX 2016001396 A MX2016001396 A MX 2016001396A MX 2016001396 A MX2016001396 A MX 2016001396A MX 2016001396 A MX2016001396 A MX 2016001396A
- Authority
- MX
- Mexico
- Prior art keywords
- methods
- host cells
- eukaryotic host
- selection vectors
- folate receptor
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/69—Increasing the copy number of the vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/40—Systems of functionally co-operating vectors
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La invención se refiere a un vector de expresión o una combinación de al menos dos vectores de expresión que comprenden: a) un polinucleótido que codifica un receptor de folato mutado como marcador seleccionable, en donde el receptor de folato mutado tiene una disminución de la afinidad de unión de folato en comparación con el receptor de folato tipo silvestre; b) al menos un polinucleótido que codifica un polipéptido de interés, en donde cuando dicho vector de expresión o combinación de al menos dos vectores de expresión se introduce en una célula hospedera, el polipéptido de interés se secreta a partir de dicha célula hospedera. También se proporcionan células hospederas adecuadas, métodos de selección y métodos para la producción de polipéptidos con alto rendimiento.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361860439P | 2013-07-31 | 2013-07-31 | |
| PCT/IB2014/063517 WO2015015419A1 (en) | 2013-07-31 | 2014-07-29 | Novel selection vectors and methods of selecting eukaryotic host cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2016001396A true MX2016001396A (es) | 2016-05-05 |
| MX369109B MX369109B (es) | 2019-10-29 |
Family
ID=51383909
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2016001396A MX369109B (es) | 2013-07-31 | 2014-07-29 | Selección novedosa de vectores y métodos de selección de células hospederas eucarióticas. |
Country Status (19)
| Country | Link |
|---|---|
| US (1) | US11807855B2 (es) |
| EP (3) | EP3412684B1 (es) |
| JP (1) | JP6576924B2 (es) |
| KR (1) | KR102288233B1 (es) |
| CN (2) | CN105593239B (es) |
| AU (1) | AU2014297966B2 (es) |
| BR (1) | BR112016001182A2 (es) |
| CA (1) | CA2918117C (es) |
| DK (2) | DK3412684T3 (es) |
| ES (2) | ES2920510T3 (es) |
| HU (1) | HUE058885T2 (es) |
| IL (1) | IL243352B (es) |
| LT (2) | LT3412684T (es) |
| MX (1) | MX369109B (es) |
| PL (2) | PL3027646T3 (es) |
| PT (2) | PT3412684T (es) |
| RU (1) | RU2716977C2 (es) |
| SG (1) | SG11201510625TA (es) |
| WO (1) | WO2015015419A1 (es) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX2011009025A (es) | 2009-02-27 | 2011-09-28 | Novartis Ag | Sistema de vector de expresion que comprende dos marcadores de seleccion. |
| DK3412684T3 (da) | 2013-07-31 | 2022-07-04 | Novartis Ag | Nye selektionsvektorer og fremgangsmåder til selektering af eukaryotiske værtsceller |
| EP3521435A1 (en) | 2018-02-06 | 2019-08-07 | Bayer AG | A high stringency selection system for protein expression in proline-auxotrophic cells |
| WO2020051331A1 (en) * | 2018-09-07 | 2020-03-12 | Medimmune, Llc | Methods of cell selection |
| TW202124445A (zh) | 2019-09-18 | 2021-07-01 | 瑞士商諾華公司 | Ｎｋｇ２ｄ融合蛋白及其用途 |
| WO2022107090A1 (en) | 2020-11-23 | 2022-05-27 | Novartis Ag | Expression technology for antibody constructs |
| WO2022229854A1 (en) | 2021-04-29 | 2022-11-03 | Novartis Ag | Hypersialylating cells |
| WO2023122567A1 (en) * | 2021-12-21 | 2023-06-29 | Absci Corporation | Fola selection assay to identify strains with increased soluble target protein expression |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4771036A (en) | 1986-02-10 | 1988-09-13 | Trustees Of Columbia University In The City Of New York | Method and ophthalmic composition for the prevention and reversal of cataracts |
| JP2585532B2 (ja) | 1986-05-10 | 1997-02-26 | 三井東圧化学株式会社 | 動物細胞の形質転換体及びそれを得る方法並びにその形質転換体を用いてｔ−ＰＡを生産する方法 |
| CA2096222C (en) | 1990-11-13 | 1998-12-29 | Stephen D. Lupton | Bifunctional selectable fusion genes |
| ATE199023T1 (de) | 1991-03-29 | 2001-02-15 | Genentech Inc | Methoden zur selektion von rekombinanten wirtszellen welche hohe mengen von einem gewünschten protein exprimieren |
| EP0804590A1 (en) | 1993-05-21 | 1997-11-05 | Targeted Genetics Corporation | Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene |
| US5439808A (en) | 1993-07-23 | 1995-08-08 | North American Vaccine, Inc. | Method for the high level expression, purification and refolding of the outer membrane group B porin proteins from Neisseria meningitidis |
| US5830698A (en) | 1997-03-14 | 1998-11-03 | Idec Pharmaceuticals Corporation | Method for integrating genes at specific sites in mammalian cells via homologous recombination and vectors for accomplishing the same |
| US6106825A (en) | 1997-05-07 | 2000-08-22 | University Of Florida | Entomopoxvirus-vertebrate gene delivery vector and method |
| FR2784030B1 (fr) | 1998-10-02 | 2002-12-20 | Inst Nat Sante Rech Med | Utilisation de bloqueurs des canaux calciques et/ou cgmp-dependants pour le traitement de pathologies de la retine |
| FR2823221B1 (fr) | 2001-04-06 | 2004-04-02 | Univ Pasteur | Sequences associees a la degenerescence retinienne et applications |
| US7384744B2 (en) | 2002-11-29 | 2008-06-10 | Boehringer Ingelheim Pharma Gmbh & Co., Kg | Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products |
| DE10256083A1 (de) | 2002-11-29 | 2004-08-12 | Boehringer Ingelheim Pharma Gmbh & Co. Kg | Expressionsvektor, Verfahren zur Herstellung von heterologen Genprodukten und Selektionsverfahren für hochproduzierende rekombinante Zellen |
| US7824907B2 (en) * | 2003-03-11 | 2010-11-02 | Merck Serono Sa | Expression vectors comprising the mCMV IE2 promoter |
| ATE440949T1 (de) | 2004-01-30 | 2009-09-15 | Maxygen Holdings Ltd | Gesteuertes überlesen von stopcodons |
| US20060275794A1 (en) | 2005-03-07 | 2006-12-07 | Invitrogen Corporation | Collections of matched biological reagents and methods for identifying matched reagents |
| EP1987150B1 (en) | 2006-02-21 | 2011-06-01 | ChromaGenics B.V. | Selection of host cells expressing protein at high levels |
| EP2208792B1 (en) | 2006-05-17 | 2015-05-06 | Hoffmann-La Roche AG | Polypeptide producing cells |
| PL2227549T3 (pl) | 2007-12-21 | 2015-12-31 | Novartis Ag | System selekcji do hodowli komórek eukariotycznych oparty na genie związanego z błoną receptora folianowego |
| EP2227546B1 (en) | 2007-12-21 | 2016-05-04 | Novartis AG | Mammalian expression vector |
| AU2009286956B2 (en) | 2008-08-28 | 2012-12-06 | Novartis Ag | Cell surface display of polypeptide isoforms by stop codon readthrough |
| EP2328917B1 (en) | 2008-09-10 | 2015-09-02 | INSERM - Institut National de la Santé et de la Recherche Médicale | Neuronal viability factor and use thereof |
| EP2401383B1 (en) | 2009-02-27 | 2013-09-18 | Novartis AG | Methods for selecting eukaryotic cells expressing a heterologous protein |
| MX2011009025A (es) | 2009-02-27 | 2011-09-28 | Novartis Ag | Sistema de vector de expresion que comprende dos marcadores de seleccion. |
| CN102030754B (zh) * | 2009-09-28 | 2013-07-10 | 中国科学院微生物研究所 | 非病毒载体及其制备方法与应用 |
| DK3412684T3 (da) | 2013-07-31 | 2022-07-04 | Novartis Ag | Nye selektionsvektorer og fremgangsmåder til selektering af eukaryotiske værtsceller |
-
2014
- 2014-07-29 DK DK18179678.0T patent/DK3412684T3/da active
- 2014-07-29 US US14/907,866 patent/US11807855B2/en active Active
- 2014-07-29 CN CN201480054263.9A patent/CN105593239B/zh active Active
- 2014-07-29 EP EP18179678.0A patent/EP3412684B1/en active Active
- 2014-07-29 PT PT181796780T patent/PT3412684T/pt unknown
- 2014-07-29 AU AU2014297966A patent/AU2014297966B2/en active Active
- 2014-07-29 PL PL14753158T patent/PL3027646T3/pl unknown
- 2014-07-29 MX MX2016001396A patent/MX369109B/es active IP Right Grant
- 2014-07-29 RU RU2016106270A patent/RU2716977C2/ru active
- 2014-07-29 EP EP14753158.6A patent/EP3027646B1/en active Active
- 2014-07-29 JP JP2016530647A patent/JP6576924B2/ja active Active
- 2014-07-29 LT LTEP18179678.0T patent/LT3412684T/lt unknown
- 2014-07-29 PT PT14753158T patent/PT3027646T/pt unknown
- 2014-07-29 SG SG11201510625TA patent/SG11201510625TA/en unknown
- 2014-07-29 DK DK14753158.6T patent/DK3027646T3/en active
- 2014-07-29 WO PCT/IB2014/063517 patent/WO2015015419A1/en active Application Filing
- 2014-07-29 LT LTEP14753158.6T patent/LT3027646T/lt unknown
- 2014-07-29 ES ES18179678T patent/ES2920510T3/es active Active
- 2014-07-29 PL PL18179678.0T patent/PL3412684T3/pl unknown
- 2014-07-29 BR BR112016001182A patent/BR112016001182A2/pt not_active Application Discontinuation
- 2014-07-29 CN CN202010947233.8A patent/CN112029791A/zh active Pending
- 2014-07-29 KR KR1020167002307A patent/KR102288233B1/ko active IP Right Grant
- 2014-07-29 CA CA2918117A patent/CA2918117C/en active Active
- 2014-07-29 ES ES14753158.6T patent/ES2689136T3/es active Active
- 2014-07-29 HU HUE18179678A patent/HUE058885T2/hu unknown
- 2014-07-29 EP EP22166577.1A patent/EP4095153A1/en not_active Withdrawn
-
2015
- 2015-12-24 IL IL243352A patent/IL243352B/en active IP Right Grant
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |