MX369109B - Selección novedosa de vectores y métodos de selección de células hospederas eucarióticas. - Google Patents
Selección novedosa de vectores y métodos de selección de células hospederas eucarióticas.Info
- Publication number
- MX369109B MX369109B MX2016001396A MX2016001396A MX369109B MX 369109 B MX369109 B MX 369109B MX 2016001396 A MX2016001396 A MX 2016001396A MX 2016001396 A MX2016001396 A MX 2016001396A MX 369109 B MX369109 B MX 369109B
- Authority
- MX
- Mexico
- Prior art keywords
- methods
- host cells
- eukaryotic host
- selection vectors
- folate receptor
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/69—Increasing the copy number of the vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/40—Systems of functionally co-operating vectors
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Toxicology (AREA)
- Cell Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
La presente invención se refiere a un vector de expresión o una combinación de al menos dos vectores de expresión que comprenden: a) un polinucleótido que codifica un receptor de folato mutado como marcador seleccionable, en donde el receptor de folato mutado tiene una disminución de la afinidad de unión de folato en comparación con el receptor de folato tipo silvestre; b) al menos un polinucleótido que codifica un polipéptido de interés, en donde cuando dicho vector de expresión o combinación de al menos dos vectores de expresión se introduce en una célula hospedera, el polipéptido de interés se secreta a partir de dicha célula hospedera. También se proporcionan células hospederas adecuadas, métodos de selección y métodos para la producción de polipéptidos con alto rendimiento.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361860439P | 2013-07-31 | 2013-07-31 | |
| PCT/IB2014/063517 WO2015015419A1 (en) | 2013-07-31 | 2014-07-29 | Novel selection vectors and methods of selecting eukaryotic host cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2016001396A MX2016001396A (es) | 2016-05-05 |
| MX369109B true MX369109B (es) | 2019-10-29 |
Family
ID=51383909
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2016001396A MX369109B (es) | 2013-07-31 | 2014-07-29 | Selección novedosa de vectores y métodos de selección de células hospederas eucarióticas. |
Country Status (19)
| Country | Link |
|---|---|
| US (1) | US11807855B2 (es) |
| EP (3) | EP3412684B1 (es) |
| JP (1) | JP6576924B2 (es) |
| KR (1) | KR102288233B1 (es) |
| CN (2) | CN105593239B (es) |
| AU (1) | AU2014297966B2 (es) |
| BR (1) | BR112016001182A2 (es) |
| CA (1) | CA2918117C (es) |
| DK (2) | DK3412684T3 (es) |
| ES (2) | ES2920510T3 (es) |
| HU (1) | HUE058885T2 (es) |
| IL (1) | IL243352B (es) |
| LT (2) | LT3027646T (es) |
| MX (1) | MX369109B (es) |
| PL (2) | PL3412684T3 (es) |
| PT (2) | PT3027646T (es) |
| RU (1) | RU2716977C2 (es) |
| SG (1) | SG11201510625TA (es) |
| WO (1) | WO2015015419A1 (es) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PT2401377T (pt) | 2009-02-27 | 2016-08-18 | Novartis Ag | Sistema de vectores de expressão compreendendo dois marcadores de selecção |
| RU2716977C2 (ru) | 2013-07-31 | 2020-03-17 | Новартис Аг | Новые селективные векторы и способы селекции эукариотических клеток-хозяев |
| EP3359666B1 (en) * | 2015-10-09 | 2025-02-19 | Genzyme Corporation | Early post-transfection isolation of cells (epic) for biologics production |
| EP3521435A1 (en) | 2018-02-06 | 2019-08-07 | Bayer AG | A high stringency selection system for protein expression in proline-auxotrophic cells |
| US20210356377A1 (en) * | 2018-09-07 | 2021-11-18 | Medimmune, Llc | Methods Of Cell Selection |
| WO2020084034A1 (en) | 2018-10-26 | 2020-04-30 | F. Hoffmann-La Roche Ag | Multispecific antibody screening method using recombinase mediated cassette exchange |
| CR20220107A (es) | 2019-09-18 | 2022-04-25 | Novartis Ag | Proteínas de fusión nkg2d y sus usos |
| JP2023550459A (ja) | 2020-11-23 | 2023-12-01 | ノバルティス アーゲー | 抗体構築物の発現技術 |
| EP4330383A1 (en) | 2021-04-29 | 2024-03-06 | Novartis AG | Hypersialylating cells |
| IL313586A (en) * | 2021-12-21 | 2024-08-01 | Absci Corp | A FOLA selection assay to identify strains with increased expression of a soluble target protein |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4771036A (en) | 1986-02-10 | 1988-09-13 | Trustees Of Columbia University In The City Of New York | Method and ophthalmic composition for the prevention and reversal of cataracts |
| JP2585532B2 (ja) | 1986-05-10 | 1997-02-26 | 三井東圧化学株式会社 | 動物細胞の形質転換体及びそれを得る方法並びにその形質転換体を用いてｔ−ＰＡを生産する方法 |
| DK0557459T3 (da) | 1990-11-13 | 1997-12-15 | Immunex Corp | Bifunktionelle, selekterbare fusionsgener |
| ATE199023T1 (de) | 1991-03-29 | 2001-02-15 | Genentech Inc | Methoden zur selektion von rekombinanten wirtszellen welche hohe mengen von einem gewünschten protein exprimieren |
| AU6953394A (en) | 1993-05-21 | 1994-12-20 | Targeted Genetics Corporation | Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene |
| US5439808A (en) | 1993-07-23 | 1995-08-08 | North American Vaccine, Inc. | Method for the high level expression, purification and refolding of the outer membrane group B porin proteins from Neisseria meningitidis |
| US5830698A (en) | 1997-03-14 | 1998-11-03 | Idec Pharmaceuticals Corporation | Method for integrating genes at specific sites in mammalian cells via homologous recombination and vectors for accomplishing the same |
| US6106825A (en) | 1997-05-07 | 2000-08-22 | University Of Florida | Entomopoxvirus-vertebrate gene delivery vector and method |
| FR2784030B1 (fr) | 1998-10-02 | 2002-12-20 | Inst Nat Sante Rech Med | Utilisation de bloqueurs des canaux calciques et/ou cgmp-dependants pour le traitement de pathologies de la retine |
| FR2823221B1 (fr) | 2001-04-06 | 2004-04-02 | Univ Pasteur | Sequences associees a la degenerescence retinienne et applications |
| DE10256083A1 (de) | 2002-11-29 | 2004-08-12 | Boehringer Ingelheim Pharma Gmbh & Co. Kg | Expressionsvektor, Verfahren zur Herstellung von heterologen Genprodukten und Selektionsverfahren für hochproduzierende rekombinante Zellen |
| US7384744B2 (en) | 2002-11-29 | 2008-06-10 | Boehringer Ingelheim Pharma Gmbh & Co., Kg | Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products |
| PT1601776E (pt) * | 2003-03-11 | 2008-09-17 | Serono Lab | Vectores de expressão que compreendem o promotor ie2 do mcmv |
| EP1716233B1 (en) | 2004-01-30 | 2009-08-26 | Maxygen Holdings Ltd. | Regulated stop codon readthrough |
| US20060275794A1 (en) | 2005-03-07 | 2006-12-07 | Invitrogen Corporation | Collections of matched biological reagents and methods for identifying matched reagents |
| CN101389763B (zh) | 2006-02-21 | 2013-01-23 | 科罗迈吉尼科斯公司 | 高水平表达蛋白质的宿主细胞的选择 |
| ES2573027T3 (es) | 2006-05-17 | 2016-06-03 | F. Hoffmann-La Roche Ag | Células productoras de polipéptido |
| EP2227546B1 (en) | 2007-12-21 | 2016-05-04 | Novartis AG | Mammalian expression vector |
| PT2227549E (pt) * | 2007-12-21 | 2015-10-08 | Novartis Ag | Sistema de seleção para cultura de células eucarióticas com base num gene de recetor de folato ligado à membrana |
| KR101734585B1 (ko) | 2008-08-28 | 2017-05-11 | 노파르티스 아게 | 정지 코돈 번역초과에 의한 폴리펩티드 이소형의 세포 표면 표시 |
| JP5748223B2 (ja) | 2008-09-10 | 2015-07-15 | アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル | ニューロン生存因子およびその使用 |
| PT2401377T (pt) | 2009-02-27 | 2016-08-18 | Novartis Ag | Sistema de vectores de expressão compreendendo dois marcadores de selecção |
| DK2401383T3 (da) | 2009-02-27 | 2013-12-16 | Novartis Ag | Fremgangsmåder til udvælgelse af eukaryote celler, som udtrykker et heterologt protein |
| CN102030754B (zh) * | 2009-09-28 | 2013-07-10 | 中国科学院微生物研究所 | 非病毒载体及其制备方法与应用 |
| RU2716977C2 (ru) | 2013-07-31 | 2020-03-17 | Новартис Аг | Новые селективные векторы и способы селекции эукариотических клеток-хозяев |
-
2014
- 2014-07-29 RU RU2016106270A patent/RU2716977C2/ru active
- 2014-07-29 DK DK18179678.0T patent/DK3412684T3/da active
- 2014-07-29 BR BR112016001182A patent/BR112016001182A2/pt not_active Application Discontinuation
- 2014-07-29 US US14/907,866 patent/US11807855B2/en active Active
- 2014-07-29 LT LTEP14753158.6T patent/LT3027646T/lt unknown
- 2014-07-29 MX MX2016001396A patent/MX369109B/es active IP Right Grant
- 2014-07-29 AU AU2014297966A patent/AU2014297966B2/en active Active
- 2014-07-29 WO PCT/IB2014/063517 patent/WO2015015419A1/en not_active Ceased
- 2014-07-29 ES ES18179678T patent/ES2920510T3/es active Active
- 2014-07-29 SG SG11201510625TA patent/SG11201510625TA/en unknown
- 2014-07-29 PT PT14753158T patent/PT3027646T/pt unknown
- 2014-07-29 JP JP2016530647A patent/JP6576924B2/ja active Active
- 2014-07-29 KR KR1020167002307A patent/KR102288233B1/ko active Active
- 2014-07-29 HU HUE18179678A patent/HUE058885T2/hu unknown
- 2014-07-29 ES ES14753158.6T patent/ES2689136T3/es active Active
- 2014-07-29 DK DK14753158.6T patent/DK3027646T3/en active
- 2014-07-29 EP EP18179678.0A patent/EP3412684B1/en active Active
- 2014-07-29 EP EP22166577.1A patent/EP4095153A1/en not_active Withdrawn
- 2014-07-29 LT LTEP18179678.0T patent/LT3412684T/lt unknown
- 2014-07-29 PT PT181796780T patent/PT3412684T/pt unknown
- 2014-07-29 PL PL18179678.0T patent/PL3412684T3/pl unknown
- 2014-07-29 CN CN201480054263.9A patent/CN105593239B/zh active Active
- 2014-07-29 PL PL14753158T patent/PL3027646T3/pl unknown
- 2014-07-29 CA CA2918117A patent/CA2918117C/en active Active
- 2014-07-29 CN CN202010947233.8A patent/CN112029791A/zh active Pending
- 2014-07-29 EP EP14753158.6A patent/EP3027646B1/en active Active
-
2015
- 2015-12-24 IL IL243352A patent/IL243352B/en active IP Right Grant
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| MX2016001396A (es) | Seleccion novedosa de vectores y metodos de seleccion de celulas hospederas eucarioticas. | |
| MX2025006260A (es) | Variantes de desoxirribonucleasa (dnasa) | |
| MX2023001394A (es) | Genes del factor viii optimizados. | |
| PH12019500781A1 (en) | IL15/IL15Ra HETERODIMERIC FC-FUSION PROTEINS | |
| MX2021014712A (es) | Polipeptidos de transposasa y sus usos. | |
| EP3950941A3 (en) | Dnase polypeptide variants | |
| MX338068B (es) | Polipeptidos que tienen actividad de glucoamilasa y polinucleotidos que codifican los mismos. | |
| MX2019000643A (es) | Receptores de antigenos quimericos y metodos de uso. | |
| IN2014CN04586A (es) | ||
| MX2015000312A (es) | Polipeptidos que tienen actividad lipasa y polinucleotidos que los codifican. | |
| MX2017014125A (es) | Variantes de alfa-amilasa y polinucleotidos que codifican las mismas. | |
| EP3741848A3 (en) | Protease variants and polynucleotides encoding same | |
| MX337985B (es) | Polipeptidos que tienen actividad de celobiohidrolasa y polinucleotidos que codifican los mismos. | |
| MX337919B (es) | Polipeptidos que tienen actividad de celobiohidrolasa y polinucleotidos que codifican los mismos. | |
| WO2016062875A3 (en) | Glucoamylase variants and polynucleotides encoding same | |
| MX337913B (es) | Polipeptidos que tienen actividad celobiohidrolasa y polinucleotidos que codifican los mismos. | |
| MX371025B (es) | Células eucariotas novedosas y métodos para la expresión recombinante de un producto de interés. | |
| EP4603591A3 (en) | Polypeptides having d-psicose 3-epimerase activity and polynucleotides encoding same | |
| MX2020003508A (es) | Un factor de secrecion de proteinas con alta eficacia secretora y un vector de expresion que lo comprende. | |
| MX2018010851A (es) | Anticuerpos de gitr, métodos, y usos. | |
| EP3964575A3 (en) | Alpha-amylase variants and polynucleotides encoding same | |
| MX349517B (es) | Polipeptidos que tienen actividad de proteasa y polinucleotidos que codifican los mismos. | |
| MX2016011194A (es) | Construcciones de expresion y metodos para seleccionar celulas huesped que expresan polipeptidos. | |
| PH12016501689B1 (en) | Tatk-cdkl5 fusion proteins, compositions, formulations, and use thereof | |
| MX2014003403A (es) | Polipeptidos que poseen actividad proteasa y polinucleotidos que los codifican. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |