MX371025B - Células eucariotas novedosas y métodos para la expresión recombinante de un producto de interés. - Google Patents

Células eucariotas novedosas y métodos para la expresión recombinante de un producto de interés.

Info

Publication number
MX371025B
MX371025B MX2016008249A MX2016008249A MX371025B MX 371025 B MX371025 B MX 371025B MX 2016008249 A MX2016008249 A MX 2016008249A MX 2016008249 A MX2016008249 A MX 2016008249A MX 371025 B MX371025 B MX 371025B
Authority
MX
Mexico
Prior art keywords
interest
product
recombinantly expressing
methods
eukaryotic cells
Prior art date
Application number
MX2016008249A
Other languages
English (en)
Other versions
MX2016008249A (es
Inventor
Jostock Thomas
Laux Holger
Ritter Anett
Original Assignee
Novartis Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=52278687&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX371025(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novartis Ag filed Critical Novartis Ag
Publication of MX2016008249A publication Critical patent/MX2016008249A/es
Publication of MX371025B publication Critical patent/MX371025B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere a una célula eucariota novedosa adecuada para la producción recombinante de un producto de interés, en donde el efecto del producto de la expresión de un gen C12orf35 endógeno se altera en dicha célula, incrementando por tanto su productividad cuando expresa de manera recombinante un polipéptido de interés. Adicionalmente, la presente invención proporciona tecnologías asociadas en donde tales células huéspedes son usadas en tecnologías de producción recombinante.
MX2016008249A 2013-12-20 2014-12-18 Células eucariotas novedosas y métodos para la expresión recombinante de un producto de interés. MX371025B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361919313P 2013-12-20 2013-12-20
PCT/IB2014/067073 WO2015092735A1 (en) 2013-12-20 2014-12-18 Novel eukaryotic cells and methods for recombinantly expressing a product of interest

Publications (2)

Publication Number Publication Date
MX2016008249A MX2016008249A (es) 2016-09-29
MX371025B true MX371025B (es) 2020-01-13

Family

ID=52278687

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016008249A MX371025B (es) 2013-12-20 2014-12-18 Células eucariotas novedosas y métodos para la expresión recombinante de un producto de interés.

Country Status (18)

Country Link
US (2) US11203631B2 (es)
EP (2) EP3083676B1 (es)
JP (1) JP6708548B2 (es)
KR (1) KR102288857B1 (es)
CN (1) CN106029692A (es)
AU (1) AU2014369175B2 (es)
BR (1) BR112016013443B1 (es)
CA (1) CA2934411C (es)
DK (1) DK3083676T3 (es)
ES (1) ES2758504T3 (es)
IL (1) IL245854B (es)
LT (1) LT3083676T (es)
MX (1) MX371025B (es)
PL (1) PL3083676T3 (es)
PT (1) PT3083676T (es)
RU (1) RU2720525C2 (es)
SG (1) SG11201604215XA (es)
WO (1) WO2015092735A1 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9963511B2 (en) * 2011-12-22 2018-05-08 Hoffmann-La Roche Inc. Expression vector organization, novel production cell generation methods and their use for the recombinant production of polypeptides
ES2758505T3 (es) 2013-12-20 2020-05-05 Novartis Ag Células eucariotas novedosas y métodos para expresar de forma recombinante un producto de interés
KR102288857B1 (ko) 2013-12-20 2021-08-11 노파르티스 아게 신규한 진핵 세포 및 관심 생성물의 재조합 발현 방법
EP3015475A1 (en) 2014-10-31 2016-05-04 Novartis AG Mammalian cells expressing cytomegalovirus antigens
EP3048114A1 (en) 2015-01-22 2016-07-27 Novartis AG Cytomegalovirus antigens and uses thereof
KR20180096793A (ko) * 2016-01-14 2018-08-29 안톤 바우어 생산 세포주를 제조하는 방법
UY37758A (es) 2017-06-12 2019-01-31 Novartis Ag Método de fabricación de anticuerpos biespecíficos, anticuerpos biespecíficos y uso terapéutico de dichos anticuerpos
CN107723276B (zh) * 2017-11-02 2021-08-13 上海交通大学 一种稳定高表达目标产物的细胞株的构建方法和试剂盒
CN107828738A (zh) * 2017-11-28 2018-03-23 新乡医学院 一种dna甲基转移酶缺陷型cho细胞系及其制备方法及应用
UY38299A (es) 2018-07-18 2020-02-28 Novartis Ag Apirasas solubilizadas, métodos y usos
KR102655024B1 (ko) * 2019-04-02 2024-04-04 추가이 세이야쿠 가부시키가이샤 표적 특이적인 외래 유전자의 도입 방법
CN112852874B (zh) * 2021-02-04 2023-05-23 中国农业科学院兰州兽医研究所 Hdac5基因敲除的bhk-21细胞系及其构建方法和应用

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2585532B2 (ja) 1986-05-10 1997-02-26 三井東圧化学株式会社 動物細胞の形質転換体及びそれを得る方法並びにその形質転換体を用いてｔ−ＰＡを生産する方法
DE69128037T2 (de) 1990-11-13 1998-05-07 Immunex Corp Bifunktionelle wählbare fusionsgene
ATE199023T1 (de) 1991-03-29 2001-02-15 Genentech Inc Methoden zur selektion von rekombinanten wirtszellen welche hohe mengen von einem gewünschten protein exprimieren
AU6953394A (en) 1993-05-21 1994-12-20 Targeted Genetics Corporation Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene
JP4031154B2 (ja) 1999-07-30 2008-01-09 独立行政法人科学技術振興機構 遺伝子増幅細胞の迅速選択法
JP2004538002A (ja) 2001-08-02 2004-12-24 アルタナ　ファルマ　アクチエンゲゼルシャフト 停止コドン抑制による組み換え遺伝子発現の新規方法
WO2003099996A2 (en) 2002-05-22 2003-12-04 Biogen Idec Ma Inc. Detection of secreted polypeptides
AU2003294217A1 (en) 2002-08-29 2004-05-04 Five Prime Therapeutics, Inc. Human polypeptides encoded by polynucleotides and methods of their use
BRPI0408246A (pt) 2003-03-11 2006-03-01 Applied Research Systems vetores de expressão que compreendem o promotor ie2 do mcmv
EP1716233B1 (en) 2004-01-30 2009-08-26 Maxygen Holdings Ltd. Regulated stop codon readthrough
US20050272061A1 (en) 2004-02-19 2005-12-08 Seattle Genetics, Inc. Expression profiling in non-small cell lung cancer
IL165484A0 (en) 2004-11-30 2006-01-15 Applied Research Systems Production of proteins
JP4870208B2 (ja) 2006-05-17 2012-02-08 エフ．ホフマン−ラ ロシュ アーゲー ポリペプチド産生細胞
EP2078075B1 (en) * 2006-10-30 2015-09-02 Eli Lilly and Company Random homozygous gene perturbation to enhance antibody production
WO2008064095A1 (en) 2006-11-17 2008-05-29 3M Innovative Properties Company Ribbon curling device
EP2115145A1 (en) * 2007-02-15 2009-11-11 DSM IP Assets B.V. A recombinant host cell for the production of a compound of interest
US8697397B2 (en) * 2007-08-07 2014-04-15 Chugai Seiyaku Kabushiki Kaisha Method of producing heterogeneous protein
AU2008340017B2 (en) 2007-12-21 2012-04-05 Novartis Ag Organic compounds
RU2389628C1 (ru) * 2008-08-18 2010-05-20 Закрытое акционерное общество Научная организация "Тверской институт вагоностроения" (ЗАО НО "ТИВ") Привод тормоза вагона
JP5788796B2 (ja) 2008-08-28 2015-10-07 ノバルティス アーゲー 終止コドンのリードスルーによるポリペプチドアイソフォームの細胞表面提示
JP5801207B2 (ja) 2009-02-27 2015-10-28 ノバルティス アーゲー ２種類の選択マーカーを含む発現ベクター系
EP2430159A1 (en) 2009-05-15 2012-03-21 Boehringer Ingelheim International GmbH Improved cell lines having reduced expression of nocr and use thereof
KR102288857B1 (ko) 2013-12-20 2021-08-11 노파르티스 아게 신규한 진핵 세포 및 관심 생성물의 재조합 발현 방법
ES2758505T3 (es) 2013-12-20 2020-05-05 Novartis Ag Células eucariotas novedosas y métodos para expresar de forma recombinante un producto de interés

Also Published As

Publication number Publication date
LT3083676T (lt) 2019-11-25
PT3083676T (pt) 2019-11-20
RU2016129285A (ru) 2018-01-25
BR112016013443B1 (pt) 2022-11-29
JP6708548B2 (ja) 2020-06-10
CA2934411A1 (en) 2015-06-25
DK3083676T3 (da) 2019-11-25
JP2017500868A (ja) 2017-01-12
AU2014369175B2 (en) 2017-03-02
WO2015092735A1 (en) 2015-06-25
ES2758504T3 (es) 2020-05-05
EP3083676A1 (en) 2016-10-26
US20170002059A1 (en) 2017-01-05
RU2720525C2 (ru) 2020-04-30
SG11201604215XA (en) 2016-07-28
KR20160101981A (ko) 2016-08-26
IL245854A0 (en) 2016-07-31
US20220298228A1 (en) 2022-09-22
PL3083676T3 (pl) 2020-02-28
KR102288857B1 (ko) 2021-08-11
BR112016013443A2 (pt) 2017-09-26
EP3604331A1 (en) 2020-02-05
EP3083676B1 (en) 2019-08-28
MX2016008249A (es) 2016-09-29
US11203631B2 (en) 2021-12-21
CN106029692A (zh) 2016-10-12
IL245854B (en) 2021-06-30
AU2014369175A1 (en) 2016-06-16
CA2934411C (en) 2022-05-03

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