ES2543629T3 - Células productoras de polipéptidos - Google Patents
Células productoras de polipéptidos Download PDFInfo
- Publication number
- ES2543629T3 ES2543629T3 ES10154684.4T ES10154684T ES2543629T3 ES 2543629 T3 ES2543629 T3 ES 2543629T3 ES 10154684 T ES10154684 T ES 10154684T ES 2543629 T3 ES2543629 T3 ES 2543629T3
- Authority
- ES
- Spain
- Prior art keywords
- immunoglobulin
- nucleic acid
- mrna
- domain
- processing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 229920001184 polypeptide Polymers 0.000 title 1
- 102000004196 processed proteins & peptides Human genes 0.000 title 1
- 108090000765 processed proteins & peptides Proteins 0.000 title 1
- 108060003951 Immunoglobulin Proteins 0.000 abstract 6
- 102000018358 immunoglobulin Human genes 0.000 abstract 6
- 108020004707 nucleic acids Proteins 0.000 abstract 5
- 150000007523 nucleic acids Chemical class 0.000 abstract 5
- 102000039446 nucleic acids Human genes 0.000 abstract 5
- 210000004027 cell Anatomy 0.000 abstract 4
- 108020004999 messenger RNA Proteins 0.000 abstract 4
- 102000019260 B-Cell Antigen Receptors Human genes 0.000 abstract 2
- 108010012919 B-Cell Antigen Receptors Proteins 0.000 abstract 2
- 108020004705 Codon Proteins 0.000 abstract 2
- 210000000170 cell membrane Anatomy 0.000 abstract 2
- 210000003527 eukaryotic cell Anatomy 0.000 abstract 2
- 239000012634 fragment Substances 0.000 abstract 2
- 102000006496 Immunoglobulin Heavy Chains Human genes 0.000 abstract 1
- 108010019476 Immunoglobulin Heavy Chains Proteins 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000008488 polyadenylation Effects 0.000 abstract 1
- 238000010187 selection method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Método de selección de una célula eucariótica expresante de una inmunoglobulina, en el que el método comprende: a) transfectar una célula eucariótica con un ácido nucleico que comprende, en dirección 5' a 3', i) un primer ácido nucleico codificante de por lo menos un fragmento de un dominio CH3 o CH4 de cadena pesada de inmunoglobulina sin codón de parada traduccional dentro del marco, ii) un segundo ácido nucleico que se inicia con el sitio 5' donante de procesamiento de un dominio CH3 o CH4 de cadena pesada de inmunoglobulina y que termina en el sitio 3' aceptor de procesamiento del exón M1 de dominio transmembranal de cadena pesada de inmunoglobulina posterior que comprende un codón de parada traduccional dentro del marco y una señal de poliadenilación, iii) un tercer ácido nucleico codificante de: iiia) por lo menos un fragmento de un dominio transmembranal de inmunoglobulina, b) cultivar dicha célula transfectada bajo condiciones adecuadas a la producción de pre-ARNm de dicho ácido nucleico, procesar dicho pre-ARNm, y traducción de dicho ARNm procesado en una inmunoglobulina, en la que dicha célula transfectada produce inmunoglobulina soluble e inmunoglobulina unida a membrana plasmática mediante procesamiento alternativo de dicho pre-ARNm y c) seleccionar una célula con inmunoglobulina unida a membrana plasmática que será la célula expresante de una inmunoglobulina.
Description
Claims (1)
-
imagen1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP06010146 | 2006-05-17 | ||
| EP06010146 | 2006-05-17 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2543629T3 true ES2543629T3 (es) | 2015-08-20 |
Family
ID=37076089
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES07725230.2T Active ES2573027T3 (es) | 2006-05-17 | 2007-05-15 | Células productoras de polipéptido |
| ES10154684.4T Active ES2543629T3 (es) | 2006-05-17 | 2007-05-15 | Células productoras de polipéptidos |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES07725230.2T Active ES2573027T3 (es) | 2006-05-17 | 2007-05-15 | Células productoras de polipéptido |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US8354516B2 (es) |
| EP (2) | EP2208792B1 (es) |
| JP (1) | JP4870208B2 (es) |
| KR (1) | KR101202498B1 (es) |
| CN (1) | CN101410525B (es) |
| AU (1) | AU2007251766B2 (es) |
| BR (1) | BRPI0712073B8 (es) |
| CA (1) | CA2651478C (es) |
| ES (2) | ES2573027T3 (es) |
| IL (1) | IL193721A (es) |
| MX (1) | MX2008013514A (es) |
| WO (1) | WO2007131774A1 (es) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4870208B2 (ja) * | 2006-05-17 | 2012-02-08 | エフ．ホフマン−ラ ロシュ アーゲー | ポリペプチド産生細胞 |
| WO2008070367A2 (en) | 2006-11-01 | 2008-06-12 | Facet Biotech Corporation | Mammalian cell-based immunoglobulin display libraries |
| WO2009059235A2 (en) * | 2007-11-01 | 2009-05-07 | Facet Biotech Corporation | Immunoglobulin display vectors |
| ES2413379T3 (es) * | 2008-08-28 | 2013-07-16 | Novartis Ag | Presentación en la superficie celular de isoformas polipeptídicas mediante ultralectura de codón de terminación |
| MX2011009025A (es) | 2009-02-27 | 2011-09-28 | Novartis Ag | Sistema de vector de expresion que comprende dos marcadores de seleccion. |
| FR2944016B1 (fr) | 2009-04-02 | 2012-03-09 | Isp Investments Inc | Nouveaux peptides anti-age activateurs du proteasome et compositions les contenant |
| FR2944017B1 (fr) * | 2009-04-02 | 2012-03-09 | Isp Investments Inc | Nouveaux peptides eclaircissants activateurs du proteasome et compositions les contenant |
| FR2944018B1 (fr) * | 2009-04-02 | 2012-03-09 | Isp Investments Inc | Nouveaux peptides anti-age activateurs du proteasome et compositions les contenant |
| FR2944798B1 (fr) | 2009-04-23 | 2013-05-10 | Isp Investments Inc | Hydrolysats peptidiques eclaircissants activateurs du proteasome et compositions les contenant |
| FR2944797B1 (fr) | 2009-04-23 | 2013-05-10 | Isp Investments Inc | Hydrolysats peptidiques activateurs du proteasome et compositions les contenant |
| CN101875916B (zh) * | 2009-11-27 | 2013-04-17 | 西安交通大学 | 一种fgfr1高表达的重组hek293细胞及其应用 |
| AU2013349844B2 (en) | 2012-11-20 | 2017-03-02 | Novartis Ag | Optimized expression cassette for expressing a polypeptide with high yield |
| WO2014126254A1 (ja) * | 2013-02-18 | 2014-08-21 | 協和発酵キリン株式会社 | 膜結合型タンパク質発現ベクター |
| EP2970956A1 (en) | 2013-03-11 | 2016-01-20 | Novartis AG | Method of screening cell clones |
| WO2014171826A1 (en) | 2013-04-17 | 2014-10-23 | Stichting Vu-Vumc | Treatment of cognitive impairment in depressive disorders |
| ES2689136T3 (es) | 2013-07-31 | 2018-11-08 | Novartis Ag | Nuevos vectores de selección y métodos de selección de células hospedadoras eucariotas |
| PL3083677T3 (pl) | 2013-12-20 | 2020-03-31 | Novartis Ag | Nowe komórki eukariotyczne i sposoby rekombinowanej ekspresji produktu będącego przedmiotem zainteresowania |
| KR102288857B1 (ko) | 2013-12-20 | 2021-08-11 | 노파르티스 아게 | 신규한 진핵 세포 및 관심 생성물의 재조합 발현 방법 |
| BR112016019832A2 (pt) * | 2014-02-28 | 2017-10-17 | Glenmark Pharmaceuticals Sa | constructo de expressão, polinucleotídeo, vetor de expressão ou clonagem, célula hospedeira, método para produzir um polipeptídio e método para selecionar uma célula hospedeira |
| DK3320099T3 (da) * | 2015-07-09 | 2023-12-11 | Inst Nat Sante Rech Med | Membranforankret eller udskilt antistof til ekspression af lentivirale vektorer |
| CN108472314A (zh) | 2015-07-31 | 2018-08-31 | 明尼苏达大学董事会 | 修饰的细胞和治疗方法 |
| WO2018060239A1 (en) | 2016-09-29 | 2018-04-05 | F. Hoffmann-La Roche Ag | Improved method for selecting polypeptide producing cells |
| AU2017346885B2 (en) | 2016-10-18 | 2021-10-21 | Intima Bioscience, Inc. | Tumor infiltrating lymphocytes and methods of therapy |
| WO2019006418A2 (en) | 2017-06-30 | 2019-01-03 | Intima Bioscience, Inc. | ADENO-ASSOCIATED VIRAL VECTORS FOR GENE THERAPY |
| WO2019015677A1 (en) * | 2017-07-21 | 2019-01-24 | Beijing Biocytogen Co., Ltd | GENETICALLY MODIFIED IMMUNOGLOBULIN HEAVY CHAIN ANIMALS |
| CN107557367B (zh) * | 2017-09-08 | 2020-11-03 | 北京大学人民医院 | Ighg1基因突变体及其应用 |
| CN116761891A (zh) * | 2020-12-01 | 2023-09-15 | 苏黎世联邦理工学院 | 包括可变启动子的dna构建体 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4656134A (en) | 1982-01-11 | 1987-04-07 | Board Of Trustees Of Leland Stanford Jr. University | Gene amplification in eukaryotic cells |
| CA2096222C (en) | 1990-11-13 | 1998-12-29 | Stephen D. Lupton | Bifunctional selectable fusion genes |
| IL104570A0 (en) * | 1992-03-18 | 1993-05-13 | Yeda Res & Dev | Chimeric genes and cells transformed therewith |
| AU6953394A (en) | 1993-05-21 | 1994-12-20 | Targeted Genetics Corporation | Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene |
| US5888809A (en) | 1997-05-01 | 1999-03-30 | Icos Corporation | Hamster EF-1α transcriptional regulatory DNA |
| DK1196566T3 (da) | 1999-07-12 | 2006-06-06 | Genentech Inc | Ekspressionsvektorer og -metoder |
| AUPQ422399A0 (en) | 1999-11-24 | 1999-12-16 | University Of New South Wales, The | Method of screening transformed or transfected cells |
| IL136459A0 (en) * | 2000-05-30 | 2001-06-14 | Galim Galil Immunology Ltd | Antibody library |
| CA2434802C (en) | 2001-01-16 | 2013-05-28 | Regeneron Pharmaceuticals, Inc. | Isolating cells expressing secreted proteins |
| US20030033616A1 (en) | 2001-07-30 | 2003-02-13 | Star-Lack Joshua Morris | Imaging marker transgenes |
| US7384744B2 (en) | 2002-11-29 | 2008-06-10 | Boehringer Ingelheim Pharma Gmbh & Co., Kg | Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products |
| EP1477805A1 (de) | 2003-05-12 | 2004-11-17 | ProBioGen AG | Verfahren zur Zellselektion |
| EP1664774A4 (en) | 2003-08-29 | 2007-06-27 | Centocor Inc | RAPID MEANS FOR OBTAINING HIGH-EXPRESSION CLONES OF MAMMALIAN CELLS, ACCORDING TO A METHYLCELLULOSE SCREENING METHOD AND IMMUNOPRECIPITATION |
| ATE417066T1 (de) * | 2003-10-16 | 2008-12-15 | Claude Negrier | Modifizierte cdna für hohe expressionsniveaus von faktor viii und dessen derivaten |
| AU2005222963B2 (en) * | 2004-03-15 | 2009-10-29 | Biogen Idec Ma Inc. | Methods and constructs for expressing polypeptide multimers in eukaryotic cells using alternative splicing |
| JP4870208B2 (ja) * | 2006-05-17 | 2012-02-08 | エフ．ホフマン−ラ ロシュ アーゲー | ポリペプチド産生細胞 |
-
2007
- 2007-05-15 JP JP2009510343A patent/JP4870208B2/ja not_active Expired - Fee Related
- 2007-05-15 WO PCT/EP2007/004313 patent/WO2007131774A1/en active Application Filing
- 2007-05-15 EP EP20100154684 patent/EP2208792B1/en active Active
- 2007-05-15 CA CA2651478A patent/CA2651478C/en not_active Expired - Fee Related
- 2007-05-15 ES ES07725230.2T patent/ES2573027T3/es active Active
- 2007-05-15 ES ES10154684.4T patent/ES2543629T3/es active Active
- 2007-05-15 BR BRPI0712073A patent/BRPI0712073B8/pt not_active IP Right Cessation
- 2007-05-15 EP EP07725230.2A patent/EP2027273B1/en active Active
- 2007-05-15 US US12/299,918 patent/US8354516B2/en active Active
- 2007-05-15 KR KR1020087027867A patent/KR101202498B1/ko active IP Right Grant
- 2007-05-15 AU AU2007251766A patent/AU2007251766B2/en not_active Ceased
- 2007-05-15 MX MX2008013514A patent/MX2008013514A/es active IP Right Grant
- 2007-05-15 CN CN2007800108283A patent/CN101410525B/zh not_active Expired - Fee Related
-
2008
- 2008-08-27 IL IL193721A patent/IL193721A/en active IP Right Grant
-
2012
- 2012-12-05 US US13/705,339 patent/US8541204B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| KR20090003327A (ko) | 2009-01-09 |
| IL193721A (en) | 2014-09-30 |
| US20130109058A1 (en) | 2013-05-02 |
| CA2651478A1 (en) | 2007-11-22 |
| EP2208792B1 (en) | 2015-05-06 |
| MX2008013514A (es) | 2008-10-28 |
| AU2007251766B2 (en) | 2013-06-20 |
| BRPI0712073A2 (pt) | 2012-03-06 |
| EP2027273B1 (en) | 2016-04-13 |
| EP2208792A2 (en) | 2010-07-21 |
| EP2208792A3 (en) | 2010-10-27 |
| WO2007131774A1 (en) | 2007-11-22 |
| ES2573027T3 (es) | 2016-06-03 |
| US20090311725A1 (en) | 2009-12-17 |
| IL193721A0 (en) | 2011-08-01 |
| BRPI0712073B1 (pt) | 2019-11-12 |
| EP2027273A1 (en) | 2009-02-25 |
| US8354516B2 (en) | 2013-01-15 |
| US8541204B2 (en) | 2013-09-24 |
| CN101410525B (zh) | 2012-09-05 |
| JP4870208B2 (ja) | 2012-02-08 |
| CN101410525A (zh) | 2009-04-15 |
| KR101202498B1 (ko) | 2012-11-16 |
| BRPI0712073B8 (pt) | 2021-05-25 |
| JP2009537124A (ja) | 2009-10-29 |
| AU2007251766A1 (en) | 2007-11-22 |
| CA2651478C (en) | 2015-02-03 |
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