WO2015015516A3 - Expression vector for production of recombinant proteins in prokaryotic host cells - Google Patents

Expression vector for production of recombinant proteins in prokaryotic host cells Download PDF

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Publication number
WO2015015516A3
WO2015015516A3 PCT/IN2014/000504 IN2014000504W WO2015015516A3 WO 2015015516 A3 WO2015015516 A3 WO 2015015516A3 IN 2014000504 W IN2014000504 W IN 2014000504W WO 2015015516 A3 WO2015015516 A3 WO 2015015516A3
Authority
WO
WIPO (PCT)
Prior art keywords
expression vector
production
host cells
recombinant proteins
prokaryotic host
Prior art date
Application number
PCT/IN2014/000504
Other languages
French (fr)
Other versions
WO2015015516A2 (en
Inventor
Archana KRISHNAN
Sanjay Sonar
Damodar THAPPA
Original Assignee
Biogenomics Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biogenomics Limited filed Critical Biogenomics Limited
Priority to SG11201600682PA priority Critical patent/SG11201600682PA/en
Priority to EP14789419.0A priority patent/EP3027643A2/en
Priority to US14/908,791 priority patent/US9803207B2/en
Publication of WO2015015516A2 publication Critical patent/WO2015015516A2/en
Publication of WO2015015516A3 publication Critical patent/WO2015015516A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Abstract

An expression vector for production of a recombinant protein in a host cell is provided. The expression vector includes a nucleotide sequence of Sequence ID No 2 encoding for a leader peptide of sequence ID No 3.
PCT/IN2014/000504 2013-07-31 2014-07-31 Expression vector for production of recombinant proteins in prokaryotic host cells WO2015015516A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
SG11201600682PA SG11201600682PA (en) 2013-07-31 2014-07-31 Expression vector for production of recombinant proteins in prokaryotic host cells
EP14789419.0A EP3027643A2 (en) 2013-07-31 2014-07-31 Expression vector for production of recombinant proteins in prokaryotic host cells
US14/908,791 US9803207B2 (en) 2013-07-31 2014-07-31 Expression vector for production of recombinant proteins in prokaryotic host cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN2526/MUM/2013 2013-07-31
IN2526MU2013 IN2013MU02526A (en) 2013-07-31 2014-07-31

Publications (2)

Publication Number Publication Date
WO2015015516A2 WO2015015516A2 (en) 2015-02-05
WO2015015516A3 true WO2015015516A3 (en) 2015-04-02

Family

ID=51794934

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2014/000504 WO2015015516A2 (en) 2013-07-31 2014-07-31 Expression vector for production of recombinant proteins in prokaryotic host cells

Country Status (5)

Country Link
US (1) US9803207B2 (en)
EP (1) EP3027643A2 (en)
IN (1) IN2013MU02526A (en)
SG (1) SG11201600682PA (en)
WO (1) WO2015015516A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3307766A4 (en) 2015-06-11 2019-06-12 Genexine, Inc. Modified interleukin-7 protein and uses thereof
KR102386735B1 (en) 2015-11-06 2022-04-14 주식회사 제넥신 Formulation for modified interleukin-7 fusion protein
US11708399B2 (en) 2015-12-04 2023-07-25 Genexine, Inc. Pharmaceutical composition comprising immunoglobulin Fc-fused interleukin-7 fusion protein for preventing or treating human papillomavirus-caused diseases
US11357827B2 (en) 2015-12-04 2022-06-14 Genexine, Inc. Method for preventing or treating influenza virus infection using pharmaceutical composition comprising immunoglobulin Fc-fused interleukin-7 fusion protein

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001073081A1 (en) * 2000-03-31 2001-10-04 Korea Advanced Institute Of Science And Technology Escherichia coli strain secreting human granulocyte colony stimulating factor (g-csf)
US20030104628A1 (en) * 2001-09-05 2003-06-05 Nanjing University Novel method for enhancing solubility of recombinant protein products in E. coli
WO2011057237A1 (en) * 2009-11-09 2011-05-12 The Regents Of The University Of Colorado, A Body Corporate Efficient production of peptides

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3027645A2 (en) 2013-07-31 2016-06-08 Biogenomics Limited Process for production of insulin and insulin analogues

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001073081A1 (en) * 2000-03-31 2001-10-04 Korea Advanced Institute Of Science And Technology Escherichia coli strain secreting human granulocyte colony stimulating factor (g-csf)
US20030104628A1 (en) * 2001-09-05 2003-06-05 Nanjing University Novel method for enhancing solubility of recombinant protein products in E. coli
WO2011057237A1 (en) * 2009-11-09 2011-05-12 The Regents Of The University Of Colorado, A Body Corporate Efficient production of peptides

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
IZARD J W ET AL: "SIGNAL PEPTIDES: EXQUISITELY DESIGNED TRANSPORT PROMOTERS", MOLECULAR MICROBIOLOGY, WILEY-BLACKWELL PUBLISHING LTD, GB, vol. 13, no. 5, 1 January 1994 (1994-01-01), pages 765 - 773, XP009041591, ISSN: 0950-382X, DOI: 10.1111/J.1365-2958.1994.TB00469.X *
YOGENDER PAL KHASA ET AL: "Optimization of Human Granulocyte Macrophage-Colony Stimulating Factor (hGM-CSF) Expression Using Asparaginase and Xylanase Gene's Signal Sequences in", APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY ; PART A: ENZYME ENGINEERING AND BIOTECHNOLOGY, HUMANA PRESS INC, NEW YORK, vol. 165, no. 2, 12 May 2011 (2011-05-12), pages 523 - 537, XP019956391, ISSN: 1559-0291, DOI: 10.1007/S12010-011-9272-5 *

Also Published As

Publication number Publication date
EP3027643A2 (en) 2016-06-08
SG11201600682PA (en) 2016-02-26
US20160186190A1 (en) 2016-06-30
WO2015015516A2 (en) 2015-02-05
IN2013MU02526A (en) 2015-06-26
US9803207B2 (en) 2017-10-31

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