IN2015DN02771A - - Google Patents

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Publication number
IN2015DN02771A
IN2015DN02771A IN2771DEN2015A IN2015DN02771A IN 2015DN02771 A IN2015DN02771 A IN 2015DN02771A IN 2771DEN2015 A IN2771DEN2015 A IN 2771DEN2015A IN 2015DN02771 A IN2015DN02771 A IN 2015DN02771A
Authority
IN
India
Prior art keywords
nucleic acid
poi
host cell
leader
secretion
Prior art date
Application number
Inventor
Brigitte Gasser
Diethard Mattanovich
Silvia Heiss
Original Assignee
Lonza Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lonza Ag filed Critical Lonza Ag
Publication of IN2015DN02771A publication Critical patent/IN2015DN02771A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL

Abstract

An isolated nucleic acid encoding a leader which has a specific sequence an isolated leader peptide encoded by such nucleic acid an expression cassette comprising such nucleic acid encoding a leader operably linked to a nucleic acid sequence encoding a POI a recombinant yeast host cell or a vector comprising such expression cassette a method of producing a POI in such yeast host cell and further the use of the specific nucleic acid for the secretion of a POI from a host cell and/or to increase the secretion of a POI from a host cell.
IN2771DEN2015 2012-10-29 2013-10-29 IN2015DN02771A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP12190361 2012-10-29
PCT/EP2013/072572 WO2014067926A1 (en) 2012-10-29 2013-10-29 Expression sequences

Publications (1)

Publication Number Publication Date
IN2015DN02771A true IN2015DN02771A (en) 2015-09-04

Family

ID=47115533

Family Applications (1)

Application Number Title Priority Date Filing Date
IN2771DEN2015 IN2015DN02771A (en) 2012-10-29 2013-10-29

Country Status (17)

Country Link
US (2) US10160988B2 (en)
EP (2) EP3130598B1 (en)
JP (2) JP6556625B2 (en)
KR (1) KR102079293B1 (en)
CN (1) CN104837857B (en)
AU (1) AU2013341049B2 (en)
BR (1) BR112015008289A2 (en)
CA (2) CA2886460C (en)
DK (1) DK2912053T3 (en)
ES (2) ES2789299T3 (en)
HK (1) HK1208037A1 (en)
HU (1) HUE033282T2 (en)
IL (2) IL238420B (en)
IN (1) IN2015DN02771A (en)
PL (1) PL2912053T3 (en)
SG (1) SG11201502745XA (en)
WO (1) WO2014067926A1 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10160988B2 (en) * 2012-10-29 2018-12-25 Lonza Ltd Expression sequences
CN104312933B (en) * 2014-10-17 2017-03-29 江南大学 A kind of method that optimization signal peptide improves the expression of trypsin exocytosiss
US10752907B2 (en) * 2015-08-05 2020-08-25 Lonza Ltd Promoter variants
JP6974341B2 (en) 2016-03-02 2021-12-01 ロンザ リミテッドLonza Limited Improved fermentation process
JP7063486B2 (en) * 2017-03-10 2022-05-09 ボルト スレッズ インコーポレイテッド Compositions and Methods for Producing Highly Secreted Recombinant Proteins
JP2020521476A (en) 2017-05-31 2020-07-27 ウニヴェルズィテート・フューア・ボーデンクルトゥーア・ウィーン Yeast expressing the synthetic Calvin cycle
KR20200120908A (en) 2018-02-12 2020-10-22 론자 리미티드 Host cell to produce the protein of interest
CN113015782A (en) 2018-11-19 2021-06-22 巴斯夫欧洲公司 Leader sequences for yeast
US11773424B2 (en) 2019-01-11 2023-10-03 Lonza Ltd Carbon-source regulated protein production in a recombinant host cell
WO2020200414A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Protein production in mut-methylotrophic yeast
WO2020200415A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Mut- methylotrophic yeast
ES2964319T3 (en) 2019-04-01 2024-04-05 Univ Wien Bodenkultur Mut-methylotrophic yeast
CN110760003A (en) * 2019-09-10 2020-02-07 广东药科大学 Preparation method of anti-HER3 single-chain antibody
US20230126246A1 (en) 2020-04-01 2023-04-27 Lonza Ltd Helper factors for expressing proteins in yeast
US20230348545A1 (en) 2020-09-30 2023-11-02 Lonza Ltd Host cells overexpressing translational factors
EP4271794A2 (en) 2020-12-29 2023-11-08 bisy GmbH Novel yeast strains

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0118551A1 (en) 1982-09-15 1984-09-19 Collaborative Research Inc. Production of interferon in yeast by use of invertase promoter
US5010182A (en) 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
CA1340772C (en) 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
ES2180528T3 (en) 1990-01-16 2003-02-16 Ct Ingenieria Genetica Biotech METHOD OF EXPRESSION OF HETEROLOGICAL GENES IN PICHIA PASTORIS YEAST, EXPRESSION VECTORS AND TRANSFORMED MICROORGANISMS.
US5268273A (en) 1990-12-14 1993-12-07 Phillips Petroleum Company Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same
DK82893D0 (en) 1993-07-08 1993-07-08 Novo Nordisk As PEPTIDE
WO2008128701A2 (en) 2007-04-20 2008-10-30 Polymun Scientific Immunbiologische Forschung Gmbh Yeast expression systems
US7741075B2 (en) 2008-06-06 2010-06-22 Board Of Regents Of The University Of Nebraska Pichia pastoris PIR1 secretion signal peptide for recombinant protein expression and Pichia pastoris PIR1 and PIR2 anchor domain peptides for recombinant surface display
US8440456B2 (en) * 2009-05-22 2013-05-14 Vib, Vzw Nucleic acids of Pichia pastoris and use thereof for recombinant production of proteins
EP2258855A1 (en) * 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
DE102009055015B4 (en) 2009-12-18 2013-12-24 Technische Universität Dresden Yeast secretion system based on a signal sequence from the hydrophobin 1 gene sequence of Trichoderma reesei
EP3590950A1 (en) * 2011-05-09 2020-01-08 Ablynx NV Method for the production of immunoglobulin single varible domains
US10160988B2 (en) * 2012-10-29 2018-12-25 Lonza Ltd Expression sequences

Also Published As

Publication number Publication date
KR102079293B1 (en) 2020-02-19
PL2912053T3 (en) 2017-05-31
SG11201502745XA (en) 2015-05-28
IL266700A (en) 2019-07-31
EP2912053B1 (en) 2016-11-09
KR20150076160A (en) 2015-07-06
CN104837857B (en) 2018-11-06
JP2015533286A (en) 2015-11-24
HK1208037A1 (en) 2016-02-19
US10160988B2 (en) 2018-12-25
EP3130598A2 (en) 2017-02-15
US11359223B2 (en) 2022-06-14
US20180135092A1 (en) 2018-05-17
EP2912053A1 (en) 2015-09-02
IL238420B (en) 2019-05-30
CN104837857A (en) 2015-08-12
IL238420A0 (en) 2015-06-30
US20150232904A1 (en) 2015-08-20
EP3130598B1 (en) 2020-02-12
CA3212565A1 (en) 2014-05-08
JP2019141063A (en) 2019-08-29
ES2610990T3 (en) 2017-05-04
AU2013341049B2 (en) 2017-07-20
BR112015008289A2 (en) 2017-11-28
WO2014067926A1 (en) 2014-05-08
CA2886460C (en) 2023-11-14
HUE033282T2 (en) 2017-11-28
JP6556625B2 (en) 2019-08-07
CA2886460A1 (en) 2014-05-08
AU2013341049A1 (en) 2015-04-02
DK2912053T3 (en) 2017-01-30
ES2789299T3 (en) 2020-10-26
EP3130598A3 (en) 2017-05-03
IL266700B (en) 2021-08-31

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