ES2523016A1 - Vectores alfavirales y líneas celulares para la producción de proteínas recombinantes - Google Patents

Vectores alfavirales y líneas celulares para la producción de proteínas recombinantes Download PDF

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Publication number
ES2523016A1
ES2523016A1 ES201330722A ES201330722A ES2523016A1 ES 2523016 A1 ES2523016 A1 ES 2523016A1 ES 201330722 A ES201330722 A ES 201330722A ES 201330722 A ES201330722 A ES 201330722A ES 2523016 A1 ES2523016 A1 ES 2523016A1
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Spain
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interest
cell lines
alphaviral vectors
recombinant proteins
cells
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ES201330722A
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English (en)
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ES2523016B1 (es
Inventor
Alejandro ARANDA
Jaione BEZUNARTEA BEZUNARTEA
Cristian Smerdou Picazo
Dámaso MOLERO SÁNCHEZ
Edita Mistiniene
Andreu SOLDEVILA FÁBREGA
Paula SERRANO PÉREZ-NIEVAS
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3P Biopharmaceuticals SL
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3P Biopharmaceuticals SL
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Priority to ES201330722A priority Critical patent/ES2523016B1/es
Priority to PCT/ES2014/070416 priority patent/WO2014188042A1/es
Priority to ES14801304T priority patent/ES2759916T3/es
Priority to EP14801304.8A priority patent/EP3000890B1/en
Priority to US14/892,829 priority patent/US10011847B2/en
Publication of ES2523016A1 publication Critical patent/ES2523016A1/es
Application granted granted Critical
Publication of ES2523016B1 publication Critical patent/ES2523016B1/es
Expired - Fee Related legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/36011—Togaviridae
    • C12N2770/36111—Alphavirus, e.g. Sindbis virus, VEE, EEE, WEE, Semliki
    • C12N2770/36141—Use of virus, viral particle or viral elements as a vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Vectores alfavirales y líneas celulares para la producción de proteínas recombinantes. La presente invención se relaciona con polinucleótidos y vectores alfavirales para la expresión de genes de interés en células de mamífero. Adicionalmente, la invención se relaciona con células que comprenden dichos polinucleótidos y vectores alfavirales, y que son capaces de expresar de forma estable uno o más genes de interés. La invención también se relaciona con métodos para obtener dichas células, con métodos para expresar un gen de interés en dichas células, y con métodos para reemplazar el gen de interés que dichas células expresan de forma estable por otro gen de interés.
ES201330722A 2013-05-20 2013-05-20 Vectores alfavirales y líneas celulares para la producción de proteínas recombinantes Expired - Fee Related ES2523016B1 (es)

Priority Applications (5)

Application Number Priority Date Filing Date Title
ES201330722A ES2523016B1 (es) 2013-05-20 2013-05-20 Vectores alfavirales y líneas celulares para la producción de proteínas recombinantes
PCT/ES2014/070416 WO2014188042A1 (es) 2013-05-20 2014-05-20 Vectores alfavirales y líneas celulares para la producción de proteínas recombinantes
ES14801304T ES2759916T3 (es) 2013-05-20 2014-05-20 Vectores alfavirales y líneas celulares para la producción de proteínas recombinantes
EP14801304.8A EP3000890B1 (en) 2013-05-20 2014-05-20 Alphaviral vectors and cell lines for producing recombinant proteins
US14/892,829 US10011847B2 (en) 2013-05-20 2014-05-20 Alphaviral vectors and cell lines for producing recombinant proteins

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201330722A ES2523016B1 (es) 2013-05-20 2013-05-20 Vectores alfavirales y líneas celulares para la producción de proteínas recombinantes

Publications (2)

Publication Number Publication Date
ES2523016A1 true ES2523016A1 (es) 2014-11-20
ES2523016B1 ES2523016B1 (es) 2015-09-09

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
ES201330722A Expired - Fee Related ES2523016B1 (es) 2013-05-20 2013-05-20 Vectores alfavirales y líneas celulares para la producción de proteínas recombinantes
ES14801304T Active ES2759916T3 (es) 2013-05-20 2014-05-20 Vectores alfavirales y líneas celulares para la producción de proteínas recombinantes

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES14801304T Active ES2759916T3 (es) 2013-05-20 2014-05-20 Vectores alfavirales y líneas celulares para la producción de proteínas recombinantes

Country Status (4)

Country Link
US (1) US10011847B2 (es)
EP (1) EP3000890B1 (es)
ES (2) ES2523016B1 (es)
WO (1) WO2014188042A1 (es)

Families Citing this family (3)

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Publication number Priority date Publication date Assignee Title
WO2020181058A1 (en) * 2019-03-05 2020-09-10 Massachusetts Institute Of Technology Dna launched rna replicon system (drep) and uses thereof
MX2022015772A (es) 2020-06-09 2023-05-19 Inozyme Pharma Inc Proteínas enpp1 o enpp3 solubles y sus usos.
CN112143755A (zh) * 2020-10-19 2020-12-29 深圳市瑞普逊科技有限公司 一种人甲状旁腺激素真核表达重组质粒载体及其构建方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011064437A2 (es) * 2009-11-26 2011-06-03 Proyecto De Biomedicina Cima, S.L. Vectores virales y procedimientos útiles en la preparación de gdnf
WO2012001196A2 (es) * 2010-06-28 2012-01-05 Proyecto De Biomedicina Cima, S.L. Vectores alfavirales y usos de los mismos para la expresión de genes heterólogos

Family Cites Families (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986004920A1 (en) 1985-02-13 1986-08-28 Biotechnology Research Partners, Limited Human metallothionein-ii promoter in mammalian expression system
US5866755A (en) 1993-06-14 1999-02-02 Basf Aktiengellschaft Animals transgenic for a tetracycline-regulated transcriptional inhibitor
GB9326271D0 (en) 1993-12-23 1994-02-23 Zeneca Ltd Expression of self-processing polyprotein in transgenic plants
DE69638321D1 (de) 1995-10-11 2011-03-03 Luminex Corp Gleichzeitige mehrfachanalyse klinischer proben
EP0910652B1 (en) 1996-04-05 2014-10-08 The Salk Institute For Biological Studies Hormone-mediated methods for modulating expression of exogenous genes in mammalian systems, and products related thereto
EP1997900A3 (en) 1996-04-05 2010-10-06 Novartis Vaccines and Diagnostics, Inc. Recombinant alphavirus-based vectors with reduced inhibition of cellular macromolecular synthesis
US6451592B1 (en) 1996-04-05 2002-09-17 Chiron Corporation Recombinant alphavirus-based vectors with reduced inhibition of cellular macromolecular synthesis
US6534314B1 (en) 1996-06-14 2003-03-18 Massachusetts Institute Of Technology Methods and compositions for transforming cells
US6057134A (en) 1996-10-07 2000-05-02 Ambion, Inc. Modulating the efficiency of nucleic acid amplification reactions with 3' modified oligonucleotides
CA2281205A1 (en) 1997-02-12 1998-08-13 Eugene Y. Chan Methods and products for analyzing polymers
US6365731B1 (en) 1997-08-06 2002-04-02 Ambion, Inc. Stripping nucleic acids with iodine and sodium thiosulfate
JP4357112B2 (ja) 1997-10-14 2009-11-04 ルミネックス コーポレイション 精密蛍光染色された粒子及びその製造及び使用方法
JP4206154B2 (ja) 1997-11-13 2009-01-07 大日本住友製薬株式会社 変異型ｌｏｘＰ配列とその応用
JP2002521461A (ja) 1998-07-29 2002-07-16 インビトロゲン・コーポレーション Ｒｎａウイルスを用いる組換えタンパク質の調節発現
US6458583B1 (en) 1998-09-09 2002-10-01 Agilent Technologies, Inc. Method and apparatus for making nucleic acid arrays
US6461812B2 (en) 1998-09-09 2002-10-08 Agilent Technologies, Inc. Method and multiple reservoir apparatus for fabrication of biomolecular arrays
US6436640B1 (en) 1999-03-18 2002-08-20 Exiqon A/S Use of LNA in mass spectrometry
WO2000060091A2 (en) 1999-04-06 2000-10-12 Oklahoma Medical Research Foundation Method for selecting recombinase variants with altered specificity
US6287813B1 (en) 1999-04-23 2001-09-11 Cistronics Cell Technology Gmbh Antibiotic-based gene regulation system
US6465183B2 (en) 1999-07-01 2002-10-15 Agilent Technologies, Inc. Multidentate arrays
US6461816B1 (en) 1999-07-09 2002-10-08 Agilent Technologies, Inc. Methods for controlling cross-hybridization in analysis of nucleic acid sequences
AU6779700A (en) 1999-08-17 2001-03-13 Luminex Corporation Microparticles with multiple fluorescent signals and methods of using same
US7122303B2 (en) 1999-09-17 2006-10-17 Agilent Technologies, Inc. Arrays comprising background features that provide for a measure of a non-specific binding and methods for using the same
US6428957B1 (en) 1999-11-08 2002-08-06 Agilent Technologies, Inc. Systems tools and methods of assaying biological materials using spatially-addressable arrays
AU1647501A (en) * 1999-12-10 2001-06-18 Cytos Biotechnology Ag Replicon based activation of endogenous genes
WO2001081553A1 (en) 2000-04-25 2001-11-01 Chiron Corporation Alphavirus-based vectors for persistent infection
US6750015B2 (en) 2000-06-28 2004-06-15 Kathryn B. Horwitz Progesterone receptor-regulated gene expression and methods related thereto
AU2002334307A1 (en) 2001-09-04 2003-03-18 Exiqon A/S Novel lna compositions and uses thereof
DE10148294C1 (de) 2001-09-29 2003-01-16 Unicor Rohrsysteme Gmbh Formbackenhälfte für einen Corrugator zum Herstellen von Querrippenrohren
EP1534737A4 (en) 2002-04-29 2007-10-31 Univ Saint Louis PEPTIDE ANTAGONISTS OF TGF-BETA FAMILY ELEMENTS AND THERAPEUTIC USES OF THESE ANTAGONISTS
ES2304069B1 (es) 2003-08-22 2009-08-12 Proyecto De Biomedicina Cima, S.L. Peptidos con capacidad de unirse al factor transformante de crecimiento beta 1 (tgf-b1).
ES2292271B1 (es) 2004-05-20 2009-02-16 Proyecto De Biomedicina Cima, S.L. Un vector hibrido adenovirus-alfavirus para la administracion eficaz y expresion de genes terapeuticos en celulas tumorales.
JP2009524406A (ja) 2005-10-13 2009-07-02 ビーシー キャンサー エージェンシー 合成生物学および代謝工学のためのモジュラー型ゲノム
GR20060100496A (el) * 2006-09-01 2008-04-15 Παρθενιος Μπουλικας ΛΙΠΟΣΩΜΑΤΙΚΑ ΕΓΚΑΨΥΛΙΩΜΕΝΟΙ ΦΟΡΕΙΣ-ΥΒΡΙΔΙΟΥ ΑΔΕΝΟΙΟΥ - ΙΟΥ SEMLIKI FOREST (SFV), ΠΟΥ ΦΕΡΕΙ ΚΑΤΑΣΚΕΥΑΣΜΑΤΑ RNAi ΚΑΙ ΘΕΡΑΠΕΥΤΙΚΑ ΓΟΝΙΔΙΑ ΠΟΥ ΧΡΗΣΙΜΟΠΟΙΟΥΝΤΑΙ ΕΝΑΝΤΙΟΝ ΣΤΟΧΩΝ ΚΑΡΚΙΝΟΥ ΚΑΙ ΑΛΛΩΝ ΑΣΘΕΝΕΙΩΝ
RS51484B (en) 2006-11-28 2011-04-30 Proyecto De Biomedicina Cima, S.L. VIRAL VECTOR AND USES OF THE SAME

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011064437A2 (es) * 2009-11-26 2011-06-03 Proyecto De Biomedicina Cima, S.L. Vectores virales y procedimientos útiles en la preparación de gdnf
WO2012001196A2 (es) * 2010-06-28 2012-01-05 Proyecto De Biomedicina Cima, S.L. Vectores alfavirales y usos de los mismos para la expresión de genes heterólogos

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
GEISS, B.J. et al., `Recombination-ready Sindbis replicon expression vectors for transgene expression¿, VIROLOGY JOURNAL, 2007, Vol. 4, Página 112, ISSN: 1743-422X (Electronic), doi: 10.1186/1743-422X-4-112, Materiales y Métodos, Figura 1. *
KARLSSON, G.B. et al., `Delivery and expression of heterologous genes in mammalian cells using self-replicating alphavirus vectors¿, METHODS MOL. BIOL., 2004, Vol. 246, Páginas 543-557, ISSN: 1064-3745 (Print), todo el documento. *
QUETGLAS, J.I. et al., `Alphavirus vectors for cancer therapy¿, VIRUS RESEARCH, 2010 Nov, Vol. 153, No. 2, Páginas 179-196, ISSN: 0168-1702, doi: 10.1016/j.virusres.2010.07.027, todo el documento. *
RIEZEBOS-BRILMAN, A. et al., `Recombinant alphaviruses as vectors for anti-tumour and anti-microbial immunotherapy¿, JOURNAL OF CLINICAL VIROLOGY, 2006, Vol. 35, No. 3, Páginas 233-243, ISSN: 1386-6532 (Print), todo el documento. *
STEEL, J.J. et al., `Infectious alphavirus production from a simple plasmid transfection+¿, VIROLOGY JOURNAL, 2011, Vol. 8, Página 356, ISSN: 1743-422X (Electronic), doi: 10.1186/1743-422X-8-356, Métodos, Figura 1. *
WANG, Y. et al., `Recombinase technology: applications and possibilities¿, Plant Cell Reports, 2011, Vol. 30, No. 3, Páginas 267-285, ISSN: 0721-7714, todo el documento. *

Also Published As

Publication number Publication date
ES2759916T3 (es) 2020-05-12
US10011847B2 (en) 2018-07-03
WO2014188042A1 (es) 2014-11-27
ES2523016B1 (es) 2015-09-09
EP3000890B1 (en) 2019-08-14
EP3000890A1 (en) 2016-03-30
EP3000890A4 (en) 2017-02-01
US20160208286A1 (en) 2016-07-21

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