MX2016008249A - Celulas eucariotas novedosas y metodos para la expresion recombinante de un producto de interes. - Google Patents

Celulas eucariotas novedosas y metodos para la expresion recombinante de un producto de interes.

Info

Publication number
MX2016008249A
MX2016008249A MX2016008249A MX2016008249A MX2016008249A MX 2016008249 A MX2016008249 A MX 2016008249A MX 2016008249 A MX2016008249 A MX 2016008249A MX 2016008249 A MX2016008249 A MX 2016008249A MX 2016008249 A MX2016008249 A MX 2016008249A
Authority
MX
Mexico
Prior art keywords
interest
product
recombinantly expressing
methods
eukaryotic cells
Prior art date
Application number
MX2016008249A
Other languages
English (en)
Other versions
MX371025B (es
Inventor
Jostock Thomas
Laux Holger
Ritter Anett
Original Assignee
Novartis Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=52278687&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2016008249(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novartis Ag filed Critical Novartis Ag
Publication of MX2016008249A publication Critical patent/MX2016008249A/es
Publication of MX371025B publication Critical patent/MX371025B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente divulgación se refiere a una célula eucariota novedosa adecuada para la producción recombinante de un producto de interés, en donde el efecto del producto de la expresión de un gen C12orf35 endógeno se altera en dicha célula, incrementando por tanto su productividad cuando expresa de manera recombinante un polipéptido de interés. Adicionalmente, la presente invención proporciona tecnologías asociadas en donde tales células huéspedes son usadas en tecnologías de producción recombinante.
MX2016008249A 2013-12-20 2014-12-18 Células eucariotas novedosas y métodos para la expresión recombinante de un producto de interés. MX371025B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361919313P 2013-12-20 2013-12-20
PCT/IB2014/067073 WO2015092735A1 (en) 2013-12-20 2014-12-18 Novel eukaryotic cells and methods for recombinantly expressing a product of interest

Publications (2)

Publication Number Publication Date
MX2016008249A true MX2016008249A (es) 2016-09-29
MX371025B MX371025B (es) 2020-01-13

Family

ID=52278687

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016008249A MX371025B (es) 2013-12-20 2014-12-18 Células eucariotas novedosas y métodos para la expresión recombinante de un producto de interés.

Country Status (18)

Country Link
US (2) US11203631B2 (es)
EP (2) EP3083676B1 (es)
JP (1) JP6708548B2 (es)
KR (1) KR102288857B1 (es)
CN (1) CN106029692A (es)
AU (1) AU2014369175B2 (es)
BR (1) BR112016013443B1 (es)
CA (1) CA2934411C (es)
DK (1) DK3083676T3 (es)
ES (1) ES2758504T3 (es)
IL (1) IL245854B (es)
LT (1) LT3083676T (es)
MX (1) MX371025B (es)
PL (1) PL3083676T3 (es)
PT (1) PT3083676T (es)
RU (1) RU2720525C2 (es)
SG (1) SG11201604215XA (es)
WO (1) WO2015092735A1 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102080356B1 (ko) * 2011-12-22 2020-02-24 에프. 호프만-라 로슈 아게 발현 벡터 구성, 신규한 생산 세포 생성 방법 및 폴리펩티드의 재조합 생산을 위한 그의 용도
ES2758504T3 (es) 2013-12-20 2020-05-05 Novartis Ag Células eucariotas novedosas y métodos para expresar de forma recombinante un producto de interés
CA2934412C (en) 2013-12-20 2024-02-20 Novartis Ag Novel eukaryotic cells and methods for recombinantly expressing a product of interest
EP3015475A1 (en) 2014-10-31 2016-05-04 Novartis AG Mammalian cells expressing cytomegalovirus antigens
EP3048114A1 (en) 2015-01-22 2016-07-27 Novartis AG Cytomegalovirus antigens and uses thereof
JP2019501658A (ja) * 2016-01-14 2019-01-24 アントン バウアーＡｎｔｏｎ Ｂａｕｅｒ 生産用細胞株を作製する方法
UY37758A (es) 2017-06-12 2019-01-31 Novartis Ag Método de fabricación de anticuerpos biespecíficos, anticuerpos biespecíficos y uso terapéutico de dichos anticuerpos
CN107723276B (zh) * 2017-11-02 2021-08-13 上海交通大学 一种稳定高表达目标产物的细胞株的构建方法和试剂盒
CN107828738A (zh) * 2017-11-28 2018-03-23 新乡医学院 一种dna甲基转移酶缺陷型cho细胞系及其制备方法及应用
UY38299A (es) 2018-07-18 2020-02-28 Novartis Ag Apirasas solubilizadas, métodos y usos
MX2021011903A (es) * 2019-04-02 2021-10-26 Chugai Pharmaceutical Co Ltd Método para introducir un gen extraño específico del objetivo.
CN112852874B (zh) * 2021-02-04 2023-05-23 中国农业科学院兰州兽医研究所 Hdac5基因敲除的bhk-21细胞系及其构建方法和应用

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2585532B2 (ja) 1986-05-10 1997-02-26 三井東圧化学株式会社 動物細胞の形質転換体及びそれを得る方法並びにその形質転換体を用いてｔ−ＰＡを生産する方法
AU650085B2 (en) 1990-11-13 1994-06-09 Immunex Corporation Bifunctional selectable fusion genes
WO1992017566A2 (en) 1991-03-29 1992-10-15 Genentech, Inc. Methods for selection of recombinant host cells expressing high levels of a desired protein
WO1994028143A1 (en) 1993-05-21 1994-12-08 Targeted Genetics Corporation Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene
JP4031154B2 (ja) * 1999-07-30 2008-01-09 独立行政法人科学技術振興機構 遺伝子増幅細胞の迅速選択法
WO2003014361A1 (en) 2001-08-02 2003-02-20 Altana Pharma Ag Novel recombinant gene expression method by stop codon suppression
CN1668634A (zh) 2002-05-22 2005-09-14 比奥根艾迪克Ma公司 分泌性多肽的检测
WO2004035732A2 (en) 2002-08-29 2004-04-29 Five Prime Therapeutics, Inc. Human polypeptides encoded by polynucleotides and methods of their use
CN100429315C (zh) 2003-03-11 2008-10-29 雪兰诺实验室有限公司 含有mcmv ie2启动子的表达载体
US20070224635A1 (en) 2004-01-30 2007-09-27 Maxygen Holdings, Ltd. Regulated Stop Codon Readthrough
US20050272061A1 (en) 2004-02-19 2005-12-08 Seattle Genetics, Inc. Expression profiling in non-small cell lung cancer
IL165484A0 (en) 2004-11-30 2006-01-15 Applied Research Systems Production of proteins
ES2543629T3 (es) 2006-05-17 2015-08-20 Hoffmann-La Roche Ag Células productoras de polipéptidos
ES2548522T3 (es) * 2006-10-30 2015-10-19 Eli Lilly And Company Perturbación genética homocigota aleatoria para mejorar la producción de anticuerpos
TWI454352B (zh) 2006-11-17 2014-10-01 3M Innovative Properties Co 帶狀物捲繞裝置
AU2008214663B2 (en) 2007-02-15 2013-10-03 Dsm Ip Assets B.V. A recombinant host cell for the production of a compound of interest
CN101809161B (zh) 2007-08-07 2014-03-12 中外制药株式会社 异种蛋白的制备方法
US20100330572A1 (en) 2007-12-21 2010-12-30 Assaraf Yehuda G Organic compounds
RU2389628C1 (ru) * 2008-08-18 2010-05-20 Закрытое акционерное общество Научная организация "Тверской институт вагоностроения" (ЗАО НО "ТИВ") Привод тормоза вагона
PL2329020T3 (pl) 2008-08-28 2013-08-30 Novartis Ag Prezentacja izoform polipeptydu na powierzchni komórki poprzez nadczytywanie kodonu stop
PT2401377T (pt) 2009-02-27 2016-08-18 Novartis Ag Sistema de vectores de expressão compreendendo dois marcadores de selecção
SG175779A1 (en) 2009-05-15 2011-12-29 Boehringer Ingelheim Int Improved cell lines having reduced expression of nocr and use thereof
ES2758504T3 (es) 2013-12-20 2020-05-05 Novartis Ag Células eucariotas novedosas y métodos para expresar de forma recombinante un producto de interés
CA2934412C (en) 2013-12-20 2024-02-20 Novartis Ag Novel eukaryotic cells and methods for recombinantly expressing a product of interest

Also Published As

Publication number Publication date
KR102288857B1 (ko) 2021-08-11
PL3083676T3 (pl) 2020-02-28
US11203631B2 (en) 2021-12-21
BR112016013443B1 (pt) 2022-11-29
AU2014369175A1 (en) 2016-06-16
CA2934411C (en) 2022-05-03
US20170002059A1 (en) 2017-01-05
JP2017500868A (ja) 2017-01-12
EP3083676B1 (en) 2019-08-28
LT3083676T (lt) 2019-11-25
IL245854A0 (en) 2016-07-31
WO2015092735A1 (en) 2015-06-25
BR112016013443A2 (pt) 2017-09-26
US20220298228A1 (en) 2022-09-22
PT3083676T (pt) 2019-11-20
CN106029692A (zh) 2016-10-12
AU2014369175B2 (en) 2017-03-02
EP3083676A1 (en) 2016-10-26
RU2720525C2 (ru) 2020-04-30
DK3083676T3 (da) 2019-11-25
ES2758504T3 (es) 2020-05-05
JP6708548B2 (ja) 2020-06-10
SG11201604215XA (en) 2016-07-28
KR20160101981A (ko) 2016-08-26
CA2934411A1 (en) 2015-06-25
EP3604331A1 (en) 2020-02-05
IL245854B (en) 2021-06-30
MX371025B (es) 2020-01-13
RU2016129285A (ru) 2018-01-25

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