SG10202103545PA - Early post-transfection isolation of cells (epic) for biologics production - Google Patents

Early post-transfection isolation of cells (epic) for biologics production

Info

Publication number
SG10202103545PA
SG10202103545PA SG10202103545PA SG10202103545PA SG10202103545PA SG 10202103545P A SG10202103545P A SG 10202103545PA SG 10202103545P A SG10202103545P A SG 10202103545PA SG 10202103545P A SG10202103545P A SG 10202103545PA SG 10202103545P A SG10202103545P A SG 10202103545PA
Authority
SG
Singapore
Prior art keywords
epic
cells
early post
biologics production
transfection isolation
Prior art date
Application number
SG10202103545PA
Inventor
Victor Cairns
Christine Demaria
Jason Vitko
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genzyme Corp filed Critical Genzyme Corp
Publication of SG10202103545PA publication Critical patent/SG10202103545PA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70592—CD52
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70596—Molecules with a "CD"-designation not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0081—Purging biological preparations of unwanted cells
    • C12N5/0087—Purging against subsets of blood cells, e.g. purging alloreactive T cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Epoxy Compounds (AREA)
SG10202103545PA 2016-10-07 2017-10-06 Early post-transfection isolation of cells (epic) for biologics production SG10202103545PA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201662405392P 2016-10-07 2016-10-07

Publications (1)

Publication Number Publication Date
SG10202103545PA true SG10202103545PA (en) 2021-05-28

Family

ID=60302441

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10202103545PA SG10202103545PA (en) 2016-10-07 2017-10-06 Early post-transfection isolation of cells (epic) for biologics production
SG11201903018WA SG11201903018WA (en) 2016-10-07 2017-10-06 Early post-transfection isolation of cells (epic) for biologics production

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG11201903018WA SG11201903018WA (en) 2016-10-07 2017-10-06 Early post-transfection isolation of cells (epic) for biologics production

Country Status (9)

Country Link
US (3) US20180119192A1 (en)
EP (1) EP3523440A1 (en)
CN (1) CN110050065B (en)
AU (2) AU2017341028B2 (en)
CA (1) CA3039598A1 (en)
MX (1) MX2019004013A (en)
SG (2) SG10202103545PA (en)
TW (2) TWI795373B (en)
WO (1) WO2018067964A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL309289A (en) 2015-10-09 2024-02-01 Genzyme Corp Improved flare (flow cytometry attenuated reporter expression) technology for rapid bulk sorting
US20180119192A1 (en) 2016-10-07 2018-05-03 Genzyme Corporation Early post-transfection isolation of cells (epic) for biologics production

Family Cites Families (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4818700A (en) 1985-10-25 1989-04-04 Phillips Petroleum Company Pichia pastoris argininosuccinate lyase gene and uses thereof
US4812405A (en) 1986-02-18 1989-03-14 Phillips Petroleum Company Double auxotrophic mutants of Pichia pastoris and methods for preparation
US4937190A (en) 1987-10-15 1990-06-26 Wisconsin Alumni Research Foundation Translation enhancer
US4929555A (en) 1987-10-19 1990-05-29 Phillips Petroleum Company Pichia transformation
CA2063799A1 (en) 1989-07-24 1991-01-25 Frank S. Genbauffe Prevention of internal initiation
US5648267A (en) 1992-11-13 1997-07-15 Idec Pharmaceuticals Corporation Impaired dominant selectable marker sequence and intronic insertion strategies for enhancement of expression of gene product and expression vector systems comprising same
WO1994026087A2 (en) 1993-05-14 1994-11-24 Connor Kim C O Recombinant protein production and insect cell culture and process
US5429746A (en) 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
US7252989B1 (en) 1994-04-04 2007-08-07 Board Of Regents, The University Of Texas System Adenovirus supervector system
US5955349A (en) 1996-08-26 1999-09-21 Zymogenetics, Inc. Compositions and methods for producing heterologous polypeptides in Pichia methanolica
US5736383A (en) 1996-08-26 1998-04-07 Zymogenetics, Inc. Preparation of Pichia methanolica auxotrophic mutants
WO1998037189A1 (en) 1997-02-25 1998-08-27 Qbi Enterprises Ltd. Ires sequences with high translational efficiency and expression vectors containing the sequence
US7001733B1 (en) 1998-05-12 2006-02-21 Rigel Pharmaceuticals, Inc. Methods and compositions for screening for modulations of IgE synthesis, secretion and switch rearrangement
US6258559B1 (en) 1999-03-22 2001-07-10 Zymogenetics, Inc. Method for producing proteins in transformed Pichia
ES2257303T3 (en) 1999-07-12 2006-08-01 Genentech, Inc. VECTORS OF EXPRESSION AND PROCEDURES.
US20050005310A1 (en) 1999-07-12 2005-01-06 Genentech, Inc. Expression vectors and methods
US20020058243A1 (en) 2000-02-02 2002-05-16 Kurt Jarnigan Rapid, parallel identification of cell lines
WO2002070710A1 (en) 2001-03-01 2002-09-12 University Of California Method to identify ires elements
DE10143237A1 (en) 2001-09-04 2003-03-20 Icon Genetics Ag Manufacture of artificial internal ribosomal entry point elements (Ires elements)
DE10143238A1 (en) 2001-09-04 2003-03-20 Icon Genetics Ag Identification of eukaryotic internal ribosome entry sites (IRES) elements
CN1668634A (en) 2002-05-22 2005-09-14 比奥根艾迪克Ma公司 Detection of secreted polypeptides
US7119187B2 (en) 2002-07-09 2006-10-10 National Health Research Institutes Internal ribosome entry site of the labial gene for protein expression
GB0216648D0 (en) 2002-07-18 2002-08-28 Lonza Biologics Plc Method of expressing recombinant protein in CHO cells
AU2003252111A1 (en) 2002-07-19 2004-02-09 Diversa Corporation Fluorescent proteins, nucleic acids encoding them and methods for making and using them
US7384744B2 (en) 2002-11-29 2008-06-10 Boehringer Ingelheim Pharma Gmbh & Co., Kg Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products
US7344886B2 (en) * 2002-11-29 2008-03-18 Boehringer Ingelheim Pharma Gmbh & Co., Kg Neomycin-phosphotransferase-genes and methods for the selection of recombinant cells producing high levels of a desired gene product
US20060173168A1 (en) 2002-12-16 2006-08-03 Wayne State University Bioactive peptides and unique ires elements from myelin proteolipid protein plp/dm20
DK2332972T3 (en) 2003-06-24 2018-04-23 Genzyme Corp Hitherto UNKNOWN BETA-ACTIN AND RPS21 PROMOTORS AND APPLICATIONS
US7776584B2 (en) 2003-08-01 2010-08-17 Genetix Limited Animal cell colony picking apparatus and method
US20070178072A1 (en) 2004-03-31 2007-08-02 Kirin Beer Kabushiki Kaisha Method for inducing differentiation of regulatory t cells usinggip-anchored protein agonist and pharmaceutical composition therefor ( as amended
US7078179B2 (en) 2004-05-04 2006-07-18 Newlink Genetics Corporation Selectable gene marker system based on expression of N-acetyllactosaminide 3-α galactosyltransferase
SI1809750T1 (en) 2004-11-08 2012-08-31 Chromagenics Bv Selection of host cells expressing protein at high levels
MX2007004595A (en) 2004-11-08 2007-08-15 Chromagenics Bv Selection of host cells expressing protein at high levels.
CA2588936A1 (en) * 2004-12-14 2006-06-22 F. Hoffmann-La Roche Ag Method for improved selection of rnai transfectants
KR101087809B1 (en) * 2006-04-10 2011-11-29 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 Systems for collection of single cells and colonies
PT2064337E (en) 2006-09-20 2012-07-26 Genzyme Corp A facs-and reporter protein-based system for high throughput development of therapeutic proteins
EP2644199B1 (en) 2007-03-05 2017-10-11 NewSouth Innovations Pty Limited Methods for detecting and modulating the sensitivity of tumour cells to anti-mitotic agents
WO2010147464A1 (en) * 2009-06-15 2010-12-23 Cellagenics B.V. Use of a cysteine synthesizing enzyme as selectable marker
EP2588862A2 (en) 2010-07-01 2013-05-08 Glaxo Group Limited Improved method for selecting high producing cell lines
DE18200782T1 (en) 2012-04-02 2021-10-21 Modernatx, Inc. MODIFIED POLYNUCLEOTIDES FOR THE PRODUCTION OF PROTEINS ASSOCIATED WITH DISEASES IN HUMANS
WO2014141037A1 (en) 2013-03-11 2014-09-18 Novartis Ag Method of screening cell clones
IL309289A (en) 2015-10-09 2024-02-01 Genzyme Corp Improved flare (flow cytometry attenuated reporter expression) technology for rapid bulk sorting
US20180119192A1 (en) 2016-10-07 2018-05-03 Genzyme Corporation Early post-transfection isolation of cells (epic) for biologics production

Also Published As

Publication number Publication date
AU2017341028A1 (en) 2019-05-23
TW201827598A (en) 2018-08-01
US20240026410A1 (en) 2024-01-25
TWI795373B (en) 2023-03-11
EP3523440A1 (en) 2019-08-14
AU2017341028B2 (en) 2023-12-21
AU2024201853A1 (en) 2024-04-11
CN110050065B (en) 2024-05-07
US20200299743A1 (en) 2020-09-24
CN110050065A (en) 2019-07-23
CA3039598A1 (en) 2018-04-12
WO2018067964A1 (en) 2018-04-12
MX2019004013A (en) 2019-08-14
US20180119192A1 (en) 2018-05-03
TW202346598A (en) 2023-12-01
SG11201903018WA (en) 2019-05-30
US11685943B2 (en) 2023-06-27

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