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  • C07D491/02—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00 in which the condensed system contains two hetero rings
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Definitions

• This invention relates to processes and intermediates for making ⁇ l- ⁇ l-[3- fluoro-2-(trifluoromethyl)isonicotinoyl]piperidin-4-yl ⁇ -3-[4-(7H-pyrrolo[2,3- d]pyrimidin-4-yl)-l H-pyrazol-l -yl]azetidin-3-yl ⁇ acetonitrile, useful in the treatment of diseases related to the activity of Janus kinases (JAK) including inflammatory disorders, autoimmune disorders, cancer, and other diseases.
• JK Janus kinases
• Protein kinases regulate diverse biological processes including cell growth, survival, differentiation, organ formation, morphogenesis, neovascularization, tissue repair, and regeneration, among others. Protein kinases also play specialized roles in a host of human diseases including cancer. Cytokines, low-molecular weight polypeptides or glycoproteins, regulate many pathways involved in the host inflammatory response to sepsis. Cytokines influence cell differentiation, proliferation and activation, and can modulate both pro-inflammatory and anti- inflammatory responses to allow the host to react appropriately to pathogens.
• JAKs Janus kinase family
• JAK2 Janus kinase- 1
• JAK2 JAK3
• TYK2 protein-tyrosine kinase 2
• Cytokine-stimulated immune and inflammatory responses contribute to pathogenesis of diseases: pathologies such as severe combined immunodeficiency (SCID) arise from suppression of the immune system, while a hyperactive or inappropriate immune/inflammatory response contributes to the pathology of autoimmune diseases (e.g., asthma, systemic lupus erythematosus, thyroiditis, myocarditis), and illnesses such as scleroderma and osteoarthritis (Ortmann, R. A., T. Cheng, et al. (2000) Arthritis Res 2(1 ): 16-32).
• SCID severe combined immunodeficiency
• JAKs Deficiencies in expression of JAKs are associated with many disease states. For example, Jakl -/- mice are runted at birth, fail to nurse, and die perinatally (Rodig, S. J., M. A. Meraz, et al. (1998) Cell 93(3): 373-83). Jak2-/- mouse embryos are anemic and die around day 12.5 postcoitum due to the absence of definitive erythropoiesis.
• the JAK/STAT pathway and in particular all four JAKs, are believed to play a role in the pathogenesis of asthmatic response, chronic obstructive pulmonary disease, bronchitis, and other related inflammatory diseases of the lower respiratory tract.
• Multiple cytokines that signal through JAKs have been linked to inflammatory diseases/conditions of the upper respiratory tract, such as those affecting the nose and sinuses (e.g., rhinitis and sinusitis) whether classically allergic reactions or not.
• the JAK/STAT pathway has also been implicated in inflammatory diseases/conditions of the eye and chronic allergic responses.
• Activation of JAK/STAT in cancers may occur by cytokine stimulation (e.g. IL-6 or GM-CSF) or by a reduction in the endogenous suppressors of JAK signaling such as SOCS (suppressor or cytokine signaling) or PIAS (protein inhibitor of activated STAT) (Boudny, V., and Kovarik, J., Neoplasm. 49:349-355, 2002).
• cytokine stimulation e.g. IL-6 or GM-CSF
• SOCS suppressor or cytokine signaling
• PIAS protein inhibitor of activated STAT
• JAK2 tyrosine kinase can be beneficial for patients with myeloproliferative disorders, e.g., polycythemia vera (PV), essential thrombocythemia (ET), myeloid metaplasia with myelofibrosis (MMM) (Levin, et al., Cancer Cell, vol. 7, 2005: 387- 397).
• PV polycythemia vera
• ET essential thrombocythemia
• MMM myeloid metaplasia with myelofibrosis
• Inhibition of the JAK2V617F kinase decreases proliferation of hematopoietic cells, suggesting JAK2 as a potential target for pharmacologic inhibition in patients with PV, ET, and MMM.
• Inhibition of the JAKs may benefit patients suffering from skin immune disorders such as psoriasis, and skin sensitization.
• JAKl plays a central role in a number of cytokine and growth factor signaling pathways that, when dysregulated, can result in or contribute to disease states. For example, IL-6 levels are elevated in rheumatoid arthritis, a disease in which it has been suggested to have detrimental effects (Fonesca, J.E. et al., Autoimmunity Reviews, 8:538-42, 2009). Because IL-6 signals, at least in part, through JAKl , antagonizing IL-6 directly or indirectly through JAKl inhibition is expected to provide clinical benefit (Guschin, D., N., et al Embo J 14: 1421, 1995; Smolen, J. S., et al. Lancet 371 :987, 2008).
• JAKl is mutated resulting in constitutive undesirable tumor cell growth and survival (Mullighan CG, Proc Natl Acad Sci U S A.106:9414-8, 2009; Flex E., et al.J Exp Med. 205:751-8, 2008).
• JAKl inhibition In other autoimmune diseases and cancers elevated systemic levels of inflammatory cytokines that activate JAK 1 may also contribute to the disease and/or associated symptoms. Therefore, patients with such diseases may benefit from JAKl inhibition.
• Selective inhibitors of JAKl may be efficacious while avoiding unnecessary and potentially undesirable effects of inhibiting other JAK kinases.
• JAKl erythropoietin
• Tpo thrombopoietin
• Epo is a key growth factor for red blood cells production; hence a paucity of Epo-dependent signaling can result in reduced numbers of red blood cells and anemia (Kaushansky K, NEJM 354:2034-45, 2006).
• Tpo another example of a JAK2-dependent growth factor, plays a central role in controlling the proliferation and maturation of megakaryocytes - the cells from which platelets are produced (Kaushansky K, NEJM 354:2034-45, 2006). As such, reduced Tpo signaling would decrease megakaryocyte numbers (megakaryocytopenia) and lower circulating platelet counts (thrombocytopenia). This can result in undesirable and/or uncontrollable bleeding.
• JAK3 and Tyk2 Reduced inhibition of other JAKs, such as JAK3 and Tyk2 may also be desirable as humans lacking functional version of these kinases have been shown to suffer from numerous maladies such as severe-combined immunodeficiency or hyperimmunoglobulin E syndrome (Minegishi, Y, et al.
• JAK 1 inhibitor with reduced affinity for other JAKs would have significant advantages over a less-selective inhibitor with respect to reduced side effects involving immune suppression, anemia and thrombocytopenia.
• JAK inhibitors are described in U.S. Serial No. 13/043,986, filed March 9, 201 1 , which is incorporated herein by reference in its entirety, including ⁇ l - ⁇ l -[3- fluoro-2-(trifluoromethyl)isonicotinoyl]piperidin-4-yl ⁇ -3-[4-(7H-pyrrolo[2,3- d]pyrimidin-4-yl)-l H-pyrazol- l -yl]azetidin-3-yl ⁇ acetonitrile, which is depicted below as Formula I.
• the present invention provides, inter alia, processes and intermediates for making the compound of Formula I.
• the present invention provides processes of making a compound of Formula II:
• the present invention also provides processes of making a compound of Formula IV:
• the present invention proc compound of Formula V:
• the present invention provides a process of making a compound of Formula
• the compounds of Formula III and IV are preferably used as free bases and the compound of Formula II is produced preferably as a free base.
• free base means the non-salt form of the compound.
• the reaction of compound III and compound IV is carried out in the presence of a tertiary amine (e.g., triethylamine).
• a tertiary amine e.g., triethylamine
• the temperature of the reaction is ⁇ 30 °C.
• the reaction is carried out in a suitable solvent.
• the suitable solvent is dichloromethane.
• P 1 protecting groups include, but are not limited to the protecting groups for amines delineated in Wuts and Greene, Protective Groups in Organic Synthesis, 4th ed., John Wiley & Sons: New Jersey, pages 696-887 (and, in particular, pages 872-887) (2007), which is incorporated herein by reference in its entirety.
• P 1 is benzyloxycarbonyl (Cbz), 2,2,2-trichloroethoxycarbonyl (Troc), 2-(trimethylsilyl)ethoxycarbonyl (Teoc), 2-(4- trifluoromethylphenylsulfonyl)ethoxycarbonyl (Tsc), t-butoxycarbonyl (BOC), 1 - adamantyloxycarbonyl (Adoc), 2-adamantylcarbonyl (2-Adoc), 2,4-dimethylpent-3- yloxycarbonyl (Doc), cyclohexyloxycarbonyl (Hoc), l , l -dimethyl-2,2,2- trichloroethoxycarbonyl (TcBOC), vinyl, 2-chloroethyl, 2-phenylsulfonylethyl, ally 1, benzyl, 2-nitrobenzyl, 4-nitrobenzyl, diphenyl-4-pyridyl
• P 1 is -CH 2 OCH2CH 2 Si(CH3)3.
• the reducing agent can be any reducing agent suitable for use in reductive amination, including various borohydride and borane reducing agents, such as those in Ellen W. Baxter and Allen B. Reitz, Reductive Aminations of Carbonyl
• Non-limiting classes of appropriate reducing agents include borohydride, cyanoborohydride, tri(Ci.4 acyl)oxyborohydride (e.g., triacetoxyborohydride derivatives), 9-borobicyclo[3.3.1 ]nonane hydride, tri(Ci-4 alkyl)borohydride, and disopinocampteylcyanoborohydride derivatives, amino boranes, borane-pyridine complex, and alkylamine boranes.
• borohydride cyanoborohydride
• tri(Ci.4 acyl)oxyborohydride e.g., triacetoxyborohydride derivatives
• 9-borobicyclo[3.3.1 ]nonane hydride e.g., tri(Ci-4 alkyl)borohydride
• disopinocampteylcyanoborohydride derivatives amino boranes, borane-pyridine complex, and alkylamine boranes.
• Non-limiting examples of appropriate reducing agents include sodium cyanoborohydride, sodium triacetoxyborohydride, sodium cyano-9-borobicyclo[3.3.1 ]nonane hydride, tetrabutylammonium cyanoborohydride, cyanoborohydride on a solid support, tetramethylammonium triacetoxyborohydride, sodium triacetoxyborohydride, lithium triethylborohydride, lithium tri(sec- butyl)borohydride, sodium disopinocampteylcyanoborohydride, catechol borane, borane tetrahydrofuran, sodium borohydride, potassium borohydride, lithium borohydride, palladium in the presence of hydrogen gas, 5-ethyl-2-methylpyridine borane (PEMB), 2-picoline borane or polymer-supported triacetoxyborohydride.
• cyanoborohydride is used in combination with a titanium (IV) additive, dehydrating agent, or a zinc halide additive.
• the reducing agent is a tetra(Ci-4 alkyl)ammonium cyanoborohydride or triacetoxyborohydride, an alkali metal cyanoborohydride or triacetoxyborohydride, or an alkaline earth
• a titanium (IV) additive is a Lewis acid containing a titanium (IV) metal (e.g., titanium tetrachloride, titanium isopropoxide, titanium ethoxide, and the like).
• the process further comprisies deprotecting a compound of Formula II or said salt thereof, to form a" compound of Formula I:
• the compound of Formula I is initially produced as a free base from the free base form of the compound of Formula II.
• the deprotecting involves reacting the compound of Formula II with a suitable deprotecting agent.
• the deprotecting comprises treating with boron trifluoride etherate, followed by treating with aqueous ammonium hydroxide.
• the deprotection is carried out in a suitable solvent at a temperature of ⁇ 30 °C, ⁇ 20 °C, ⁇ 10 °C, or ⁇ 5 °C.
• the suitable solvent is acetonitrile.
• the process of deprotecting the compound of Formula II to form the compound of Formula I further comprises reacting the compound of Formula I with adipic acid to form the adipate salt.
• the process further comprises:
• Treatment of the compound of Formula II to remove the P 1 group can be accomplished by methods known in the art for the removal of particular protecting groups for amines, such as those in Wuts and Greene, Protective Groups in Organic Synthesis, 4th ed., John Wiley & Sons: New Jersey, pages 696-887 (and, in particular, pages 872-887) (2007), which is incorporated herein by reference in its entirety.
• the P 1 group is removed by treating with fluoride ion (e.g., treating with tetrabutylammonium fluoride), hydrochloric acid, pyridinium p- toluenesulfonic acid (PPTS), or a Lewis acid (e.g., lithium tetrafluoroborate)).
• the treating comprises treating with lithium tetrafluoroborate, followed by treating with ammonium hydroxide (e.g., when P 1 is 2- (trimethylsilyl)ethoxymethyl).
• the treating comprises treating with base (e.g., P 1 is N-pivaloyloxymethyl).
• the base is an alkali metal hydroxide.
• the base is sodium hydroxide.
• the treating comprises treating with sodium hydroxide or ammonia in a solvent such as methanol or water.
• the compound of Formula IV, or a salt thereof is produced by a process comprising deprotecting a compound of Formula V:
• the compound of Formula V is used preferably as a free base and the compound of Formula IV is produced preferably as a free base.
• the deprotecting comprises reacting with aqueous acid.
• the acid is hydrochloric acid.
• an excess of aqueous acid is used relative to the compound of Formula V. In some embodiments, an excess of 5, 6, 7, 8, 9, or 10 equivalents of aqueous acid is used relative to the compound of Formula V. In some embodiments, an excess of 6, 7, 8, 9, or 10 equivalents or more of aqueous acid is used relative to the compound of Formula V. In some embodiments, an excess of 7, 8, 9, or 10 equivalents or more of aqueous acid is used relative to ⁇ compound of Formula V. In some embodiments, an excess of 8, 9, or 10 equivalents or more of aqueous acid is used relative to the compound of Formula V. In some embodiments, an excess of 9 or 10 equivalents or more of aqueous acid is used relative to the compound of Formula V. In some embodiments, an excess of 9 equivalents or more of aqueous acid is used relative to the compound of Formula V. In some embodiments, an excess of 9 equivalents or more of aqueous acid is used relative to the compound of Formula V. In some embodiments, an excess of 9 equivalents or more of
• the deprotection is carried out in acetonitrile solvent at a temperature of ⁇ 30 °C, ⁇ 20 °C, ⁇ 10 °C, or ⁇ 5 °C.
• deprotecting conditions include, but are not limited to, those in Wuts and Greene, Protective Groups in Organic Synthesis, 4th ed., John Wiley & Sons: New Jersey, pages 696-887 (and, in particular, pages 872-887) (2007), which is incorporated herein by reference in its entirety.
• the compound of Formula V is produced by a process comprising reacting a compound of Formula VI: VI
• Appropriate coupling agents are any of the well-known coupling agents for coupling an amine to an acid to form an amine.
• Non-limiting examples include carbodiimides (e.g., ⁇ , ⁇ '-dicyclohexylcarbodiimide (DCC), ⁇ , ⁇ '- diisopropylcarbodiimide (D1C), l-ethyl-3-(3-dimethylaminopropyl, or
• EDC hydrochloride dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride
• EDC carbodiimide
• CDI ⁇ , ⁇ -carbonyldiimidazole
• phosphonium-based coupling agents e.g., benzotriazol-l-yloxy-tris(dimethylamino)-phosphonium hexafluorophosphate (BOP), (benzotriazol-l-yloxy)tripyrrolidinophosphonium hexafluorophosphate (P BOP), (7-azabenzotriazol- 1 -y loxy )-tris- pyrrolidinophosphonium hexafluorophosphate (PyAOP),
• BOP benzotriazol-l-yloxy-
• bromotripyrrolidinophosphonium hexafluorophosphate PyBrOP
• bis(2-oxo-3- oxazolidinyl)phosphinic chloride BOP-C1
• aminium-based reagents e.g., O- (benzotriazol-l-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate (HBTU), O- (benzotriazol-l-yl)- ⁇ , ⁇ , ⁇ ', ⁇ '-tetramethyluronium tetrafluoroborate (TBTU), 3- (diethylphosphoryloxy)-l,2,3-benzotriazin-4(3H)-one (DEPBT), 0-(7- azabenzotriazol-l -yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate (HATU), 0-(6-chloro
• TATU uronium-based reagents
• TNTU uronium-based reagents
• TNTU uronium-based reagents
• TNTU uronium-based reagents
• TNTU uronium-based reagents
• TNTU uronium-based reagents
• TNTU uronium-based reagents
• TNTU uronium-based reagents
• TNTU uronium-based reagents
• TNTU uronium-based reagents
• TNTU uronium-based reagents
• TNTU uronium-based reagents
• TNTU uronium-based reagents
• TNTU uronium-based reagents
• TNTU uronium-based reagents
• TNTU uronium-based reagents
• TNTU uronium-based reagents
• the coupling agent is benzotriazol-l-yloxy-tris(dimethylamino)- phosphonium hexafluorophosphate (BOP).
• the compounds of Formulas V, VI, and VII are, preferably, in their non-salt forms.
• the reaction of the compound of Formula VI and VII is carried out in the presence of a tertiary amine (e.g., triethylamine).
• a tertiary amine e.g., triethylamine
• the reaction is carried out in dimethylformamide (DMF) at a temperature of ⁇ 30 °C, ⁇ 20 °C, or ⁇ 15 °C.
• DMF dimethylformamide
• the coupling agent is present in > 1.05, > 1.1 , or > 1.2 equivalents relative to the compound of Formula VI.
• the compound of Formula V is a free base.
• spectroscopic means such as nuclear magnetic resonance spectroscopy (e.g., ⁇ or 13 C), infrared spectroscopy, or spectrophotometry (e.g., UV-visible); or by chromatography such as high performance liquid chromatograpy (HPLC) or thin layer chromatography (TLC) or other related techniques.
• HPLC high performance liquid chromatograpy
• TLC thin layer chromatography
• reacting is used as known in the art and generally refers to the bringing together of chemical reagents in such a manner so as to allow their interaction at the molecular level to achieve a chemical or physical transformation.
• the reacting involves two reagents, wherein one or more equivalents of second reagent are used with respect to the first reagent.
• the reacting steps of the processes described herein can be conducted for a time and under conditions suitable for preparing the identified product.
• Preparation of compounds can involve the protection and deprotection of various chemical groups.
• the need for protection and deprotection, and the selection of appropriate protecting groups can be readily determined by one skilled in the art.
• the chemistry of protecting groups can be found, for example, in Greene, et al., Protective Groups in Organic Synthesis, 4d. Ed., Wiley & Sons, 2007, which is incorporated herein by reference in its entirety. Adjustments to the protecting groups and formation and cleavage methods described herein may be adjusted as necessary in light of the various substituents.
• Suitable solvents can be substantially nonreactive with the starting materials
• reaction reactants
• reactants the intermediates, or products at the temperatures at which the reactions are carried out, e.g., temperatures which can range from the solvent's freezing temperature to the solvent's boiling temperature.
• a given reaction can be carried out in one solvent or a mixture of more than one solvent.
• suitable solvents for a particular reaction step can be selected.
• reactions can be carried out in the absence of solvent, such as when at least one of the reagents is a liquid or gas.
• Suitable solvents can include halogenated solvents such as carbon tetrachloride, bromodichloromethane, dibromochloromethane, bromoform, chloroform, bromochloromethane, dibromomethane, butyl chloride, dichloromethane, tetrachloroethylene, trichloroethylene, 1 , 1,1 -trichloroethane, 1 , 1 ,2-trichloroethane, 1 , 1-dichloroethane, 2-chloropropane, ⁇ , ⁇ , ⁇ -trifluorotoluene, 1 ,2-dichloroethane, 1 ,2- dibromoethane, hexafluorobenzene, 1 ,2,4-trichlorobenzene, 1 ,2-dichlorobenzene, chlorobenzene, fluorobenzene, mixtures thereof and the like.
• halogenated solvents such as carbon te
• Suitable ether solvents include: dimethoxymethane, tetrahydrofuran, 1 ,3- dioxane, 1 ,4-dioxane, furan, diethyl ether, ethylene glycol dimethyl ether, ethylene glycol diethyl ether, diethylene glycol dimethyl ether, diethylene glycol diethyl ether, triethylene glycol dimethyl ether, anisole, t-butyl methyl ether, mixtures thereof and the like.
• Suitable protic solvents can include, by way of example and without limitation, water, methanol, ethanol, 2-nitroethanol, 2-fluoroethanol, 2,2,2- trifluoroethanol, ethylene glycol, 1 -propanol, 2-propanol, 2-methoxyethanol, 1 - butanol, 2-butanol, i-butyl alcohol, t-butyl alcohol, 2-ethoxyethanol, diethylene glycol, 1-, 2-, or 3- pentanol, neo-pentyl alcohol, t-pentyl alcohol, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, cyclohexanol, benzyl alcohol, phenol, or glycerol.
• Suitable aprotic solvents can include, by way of example and without limitation, tetrahydrofuran (THF), ⁇ , ⁇ -dimethylformamide (DMF), N,N- dimethylacetamide (DMA), l ,3-dimethyl-3,4,5,6-tetrahydro-2(l H)-pyrimidinone (DMPU), l ,3-dimethyl-2-imidazolidinone (DMI), N-methylpyrrolidinone (NMP), formamide, N-methylacetamide, N-methylformamide, acetonitrile, dimethyl sulfoxide, propionitrile, ethyl formate, methyl acetate, hexachloroacetone, acetone, ethyl methyl ketone, ethyl acetate, sulfolane, N,N-dimethylpropionamide, tetramethylurea, nitromethane, nitrobenzene, or hexamethylphosphor
• Suitable hydrocarbon solvents include benzene, cyclohexane, pentane, hexane, toluene, cycloheptane, methylcyclohexane, heptane, ethylbenzene, m-, o-, or p- xylene, octane, indane, nonane, or naphthalene.
• reaction temperatures will depend on, for example, the melting and boiling points of the reagents and solvent, if present; the thermodynamics of the reaction (e.g., vigorously exothermic reactions may need to be carried out at reduced temperatures); and the kinetics of the reaction (e.g., a high activation energy barrier may need elevated temperatures).
• Elevated temperature refers to temperatures above room temperature (about 22 °C).
• reaction of the processes described herein can be carried out in air or under an inert atmosphere.
• reactions containing reagents or products that are substantially reactive with air can be carried out using air-sensitive synthetic techniques that are well known to the skilled artisan.
• preparation of compounds can involve the addition of acids or bases to affect, for example, catalysis of a desired reaction or formation of salt forms such as acid addition salts.
• Example acids can be inorganic or organic acids.
• Inorganic acids include hydrochloric acid, hydrobromic acid, sulfuric acid, phosphoric acid, and nitric acid.
• Organic acids include formic acid, acetic acid, propionic acid, butanoic acid, benzoic acid, 4-nitrobenzoic acid, methanesulfonic acid, p-toluenesulfonic acid,
• benzenesulfonic acid tartaric acid, trifluoroacetic acid, propiolic acid, butyric acid, 2- butynoic acid, vinyl acetic acid, pentanoic acid, hexanoic acid, heptanoic acid, octanoic acid, nonanoic acid and decanoic acid.
• Example bases include lithium hydroxide, sodium hydroxide, potassium hydroxide, lithium carbonate, sodium carbonate, and potassium carbonate.
• Some example strong bases include, but are not limited to, hydroxide, alkoxides, metal amides, metal hydrides, metal dialkylamides and arylamines, wherein; alkoxides include lithium, sodium and potassium salts of methyl, ethyl and t-butyl oxides; metal amides include sodium amide, potassium amide and lithium amide; metal hydrides include sodium hydride, potassium hydride and lithium hydride; and metal dialkylamides include sodium and potassium salts of methyl, ethyl, n-propyl, i-propyl, n-butyl, t-butyl, trimethylsilyl and cyclohexyl substituted amides.
• the intermediates and products may also include salts of the compounds disclosed herein.
• salt refers to a salt formed by the addition of an acceptable acid or base to a compound disclosed herein.
• the salts are pharmaceutically acceptable salts.
• pharmaceutically acceptable refers to a substance that is acceptable for use in pharmaceutical applications from a toxicological perspective and does not adversely interact with the active ingredient.
• Pharmaceutically acceptable salts include, but are not limited to, those derived from organic and inorganic acids such as, but not limited to, acetic, lactic, citric, cinnamic, tartaric, succinic, fumaric, maleic, malonic, mandelic, malic, oxalic, propionic, hydrochloric, hydrobromic, phosphoric, nitric, sulfuric, glycolic, pyruvic, methanesulfonic, ethanesulfonic, toluenesulfonic, salicylic, benzoic, and similarly known acceptable acids. Lists of suitable salts are found in Remington's Pharmaceutical Sciences, 17th ed., Mack Publishing Company, Easton, Pa., 1985, p.
• the usual isolation and purification operations such as concentration, filtration, extraction, solid-phase extraction, recrystallization, chromatography, and the like may be used, to isolate the desired products.
• the compounds of the invention, and salts thereof are substantially isolated.
• substantially isolated is meant that the compound is at least partially or substantially separated from the environment in which it was formed or detected.
• Partial separation can include, for example, a composition enriched in the compound of the invention.
• Substantial separation can include compositions containing at least about 50%, at least about 60%, at least about 70%, at least about
• JAK JAK inhibitors
• JAK inhibitors are useful in treating various JAK-associated diseases or disorders.
• JAK-associated diseases include diseases involving the immune system including, for example, organ transplant rejection (e.g. , allograft rejection and graft versus host disease).
• JAK-associated diseases include autoimmune diseases such as multiple sclerosis, rheumatoid arthritis, juvenile arthritis, psoriatic arthritis, type 1 diabetes, lupus, psoriasis, inflammatory bowel disease, ulcerative colitis, Crohn's disease, myasthenia gravis, immunoglobulin nephropathies, myocarditis, autoimmune thyroid disorders, chronic obstructive pulmonary disease (COPD), and the like.
• the autoimmune disease is an autoimmune bullous skin disorder such as pemphigus vulgaris (PV) or bullous pemphigoid (BP).
• JAK-associated diseases include allergic conditions such as asthma, food allergies, eszematous dermatitis, contact dermatitis, atopic dermatitis (atropic eczema), and rhinitis.
• JAK-associated diseases include viral diseases such as Epstein Barr Virus (EBV), Hepatitis B, Hepatitis C, HIV, HTLV 1 , Varicella-Zoster Virus (VZV) and Human Papilloma Virus (HPV).
• EBV Epstein Barr Virus
• Hepatitis B Hepatitis C
• HIV HTLV 1
• VZV Varicella-Zoster Virus
• HPV Human Papilloma Virus
• JAK-associated disease examples include diseases associated with cartilage turnover, for example, gouty arthritis, septic or infectious arthritis, reactive arthritis, reflex sympathetic dystrophy, algodystrophy, Tietze syndrome, costal athropathy, osteoarthritis deformans endemica, Mseleni disease, Handigodu disease, degeneration resulting from fibromyalgia, systemic lupus erythematosus, scleroderma, or ankylosing spondylitis.
• diseases associated with cartilage turnover for example, gouty arthritis, septic or infectious arthritis, reactive arthritis, reflex sympathetic dystrophy, algodystrophy, Tietze syndrome, costal athropathy, osteoarthritis deformans endemica, Mseleni disease, Handigodu disease, degeneration resulting from fibromyalgia, systemic lupus erythematosus, scleroderma, or ankylosing spondylitis.
• JAK-associated disease examples include congenital cartilage malformations, including hereditary chrondrolysis, chrondrodysplasias, and pseudochrondrodysplasias (e.g., microtia, enotia, and metaphyseal
• JAK-associated diseases or conditions include skin disorders such as psoriasis (for example, psoriasis vulgaris), atopic dermatitis, skin rash, skin irritation, skin sensitization (e.g., contact dermatitis or allergic contact dermatitis).
• skin disorders such as psoriasis (for example, psoriasis vulgaris), atopic dermatitis, skin rash, skin irritation, skin sensitization (e.g., contact dermatitis or allergic contact dermatitis).
• certain substances including some pharmaceuticals when topically applied can cause skin sensitization.
• coadministration or sequential administration of at least one JAK inhibitor of the invention together with the agent causing unwanted sensitization can be helpful in treating such unwanted sensitization or dermatitis.
• the skin disorder is treated by topical administration of at least one JAK inhibitor of the invention.
• JAK-associated diseases or conditions include those characterized by solid tumors (e.g., prostate cancer, renal cancer, hepatic cancer, pancreatic cancer, gastric cancer, breast cancer, lung cancer, cancers of the head and neck, thyroid cancer, glioblastoma, Kaposi's sarcoma, Castleman's disease, uterine leiomyosarcoma, melanoma etc.), hematological cancers (e.g., lymphoma, leukemia such as acute lymphoblastic leukemia (ALL), acute myelogenous leukemia (AML) or multiple myeloma), and skin cancer such as cutaneous T-cell lymphoma (CTCL) and cutaneous B-cell lymphoma.
• CTCLs include Sezary syndrome and mycosis fungoides.
• Other examples of JA -associated diseases or conditions include pulmonary arterial hypertension.
• the cancer is associated with inflammatory bowel disease.
• the inflammatory bowel disease is ulcerative colitis.
• the inflammatory bowel disease is Crohn's disease.
• the inflammation-associated cancer is colitis- associated cancer.
• the inflammation-associated cancer is colon cancer or colorectal cancer.
• the cancer is gastric cancer, gastrointestinal carcinoid tumor, gastrointestinal stromal tumor (GIST),
• adenocarcinoma small intestine cancer, or rectal cancer.
• JAK-associated diseases can further include those characterized by expression of: JAK2 mutants such as those having at least one mutation in the pseudo-kinase domain ⁇ e.g., JAK2V617F); JAK2 mutants having at least one mutation outside of the pseudo-kinase domain; JAK 1 mutants; JAK3 mutants; erythropoietin receptor (EPOR) mutants; or deregulated expression of CRLF2.
• JAK2 mutants such as those having at least one mutation in the pseudo-kinase domain ⁇ e.g., JAK2V617F
• JAK2 mutants having at least one mutation outside of the pseudo-kinase domain JAK 1 mutants
• JAK3 mutants JAK3 mutants
• EPOR erythropoietin receptor
• JAK-associated diseases can further include myeloproliferative disorders (MPDs) such as polycythemia vera (PV), essential thrombocythemia (ET), myelofibrosis with myeloid metaplasia (MMM), primary myelofibrosis (PMF), chronic myelogenous leukemia (CML), chronic myelomonocytic leukemia (CMML), hypereosinophilic syndrome (HES), systemic mast cell disease (SMCD), and the like.
• MPDs myeloproliferative disorders
• PV polycythemia vera
• ET essential thrombocythemia
• MMM myelofibrosis with myeloid metaplasia
• PMF primary myelofibrosis
• CML chronic myelogenous leukemia
• CMML chronic myelomonocytic leukemia
• HES hypereosinophilic syndrome
• SMCD systemic mast cell disease
• the myeloproliferative disorder is myelofibrosis (e.g., primary myelofibrosis (PMF) or post polycythemia vera/essential thrombocythemia myelofibrosis (Post-PV Post-ET MF)).
• the myeloproliferative disorder is post- essential thrombocythemia myelofibrosis (Post-ET MF).
• the myeloproliferative disorder is post polycythemia vera myelofibrosis (Post-PV MF).
• JAK-associated diseases or conditions include ameliorating the dermatological side effects of other pharmaceuticals by administration of the compound of the invention.
• numerous pharmaceutical agents result in unwanted allergic reactions which can manifest as acneiform rash or related dermatitis.
• Example pharmaceutical agents that have such undesirable side effects include anti-cancer drugs such as gefitinib, cetuximab, erlotinib, and the like.
• the compounds of the invention can be administered systemically or topically (e.g., localized to the vicinity of the dermatitis) in combination with (e.g., simultaneously or sequentially) the pharmaceutical agent having the undesirable dermatological side effect.
• the compound of the invention can be administered topically together with one or more other pharmaceuticals, where the other pharmaceuticals when topically applied in the absence of a compound of the invention cause contact dermatitis, allergic contact sensitization, or similar skin disorder.
• compositions of the invention include topical formulations containing the compound of the invention and a further pharmaceutical agent which can cause dermatitis, skin disorders, or related side effects.
• JAK-associated diseases include inflammation and inflammatory diseases.
• Example inflammatory diseases include sarcoidosis, inflammatory diseases of the eye (e.g., ulceris, uveitis, scleritis, conjunctivitis, or related disease),
• the inflammation disease of the eye is blepharitis.
• JAK-associated diseases include ischemia reperfusion injuries or a disease or condition related to an inflammatory ischemic event such as stroke or cardiac arrest, endotoxin-driven disease state (e.g., complications after bypass surgery or chronic endotoxin states contributing to chronic cardiac failure), anorexia, cachexia, fatigue such as that resulting from or associated with cancer, restenosis, sclerodermitis, fibrosis, conditions associated with hypoxia or astrogliosis such as, for example, diabetic retinopathy, cancer, or neurodegeneration, and other inflammatory diseases such as systemic inflammatory response syndrome (SIRS) and septic shock.
• SIRS systemic inflammatory response syndrome
• JAK-associated diseases include gout and increased prostate size due to, e.g., benign prostatic hypertrophy or benign prostatic hyperplasia, as well as bone resorption diseases such as osteoporosis or osteoarthritis, bone resorption diseases associated with: hormonal imbalance and/or hormonal therapy, autoimmune disease (e.g. osseous sarcoidosis), or cancer (e.g. myeloma).
• benign prostatic hypertrophy or benign prostatic hyperplasia as well as bone resorption diseases such as osteoporosis or osteoarthritis, bone resorption diseases associated with: hormonal imbalance and/or hormonal therapy, autoimmune disease (e.g. osseous sarcoidosis), or cancer (e.g. myeloma).
• autoimmune disease e.g. osseous sarcoidosis
• cancer e.g. myeloma
• dry eye disorder is intended to encompass the disease states summarized in a recent official report of the Dry Eye Workshop (DEWS), which defined dry eye as "a multifactorial disease of the tears and ocular surface that results in symptoms of discomfort, visual disturbance, and tear film instability with potential damage to the ocular surface. It is accompanied by increased osmolarity of the tear film and inflammation of the ocular surface.”
• DEWS Dry Eye Workshop
• the dry eye disorder is selected from aqueous tear-deficient dry eye (ADDE) or evaporative dry eye disorder, or appropriate combinations thereof.
• the dry eye disorder is Sjogren syndrome dry eye (SSDE).
• the dry eye disorder is non-Sjogren syndrome dry eye (NSSDE).
• JAK-associated diseases include conjunctivitis, uveitis (including chronic uveitis), chorioditis, retinitis, cyclitis, sclieritis, episcleritis, or ulceris.
• Other JAK-associated diseases include respiratory dysfunction or failure associated wth viral infection, such as influenza and SARS.
• Trimethylsilylethoxymethyl chloride (2, SEM-C1, 763 mL, 4.31 mol, 1.1 equiv) was then added slowly via an addition funnel at a rate that the internal reaction temperature did not exceed 5 °C. The reaction mixture was then stirred at 0 - 5 °C for 30 minutes. When the reaction was deemed complete determined by TLC and HPLC, the reaction mixture was quenched by water (1 L). The mixture was then diluted with water (12 L) and methyl tert-b ty] ether (MTBE) (8 L). The two layers were separated and the aqueous layer was extracted with MTBE (8 L). The combined organic layers were washed with water (2 x 4 L) and brine (4 L) and solvent switched to 1-butanol.
• MTBE methyl tert-b ty] ether
• the resulting reaction mixture was degassed three timed backfilling with nitrogen each time before being treated with tetrakis(triphenylphosphine)palladium(0) (Pd(PPli3)4, 46 g, 0.040 mol, 0.003 equiv) at room temperature.
• the resulting reaction mixture was heated to gentle reflux (about 90 °C) for 1 - 4 hours.
• the reaction mixture was gradually cooled down to room temperature before being filtered through a Ceiite bed.
• the Ceiite bed was washed with ethyl acetate (2 x 2 L) before the filtrates and washing solution were combined.
• reaction mixture was treated with a 30% aqueous sodium hydroxide (NaOH) solution (4 L, 50.42 mol, 3.78 equiv) at room temperature.
• NaOH sodium hydroxide
• the resulting reaction mixture was stirred at room temperature for 1 - 2 hours.
• the solids were collected by filtration and washed with water (2 x 5 L).
• the wet cake was charged back to the reactor with acetonitrile (21.6' L), and resulting suspension was heated to gentle reflux for 1 - 2 hours.
• the clear solution was then gradually cooled down to room temperature with stirring, and solids were precipitated out from the solution with cooling.
• the mixture was stirred at room temperature for an additional 1 - 2 hours.
• terr-butyl 3-hydroxyazetidine-l - carboxylate (10, 357 g, 393.2 g theoretical, 90.8% yield) as colorless oil, which solidified upon standing at room temperature in vacuum.
• 'iTNMR (CDCI3, 300 MHz), ⁇ 4.56 (m 1 H), 4.13 (m, 2H), 3.81 (m, 2H), 1.43 (s, 9H) ppm.
• the resulting reaction mixture was stirred for 3 hours at - 10 °C and a solution of l -/er/-butoxycarbonyl-3-azetidinone (11, 600 g, 3.50 mol) in anhydrous tetrahydrofuran (THF, 2 L) was added over 2 h keeping the internal temperature below - 5 °C.
• the reaction mixture was stirred at - 5 to - 10 °C over 1 hour and then slowly warmed up to room temperature and stirred at room temperature for overnight.
• the reaction mixture was then diluted with water (4.5 L) and saturated aqueous sodium chloride solution (NaCl, 4.5 L) and extracted with ethyl acetate (EtOAc, 2 x 9 L).
• Triethylamine (Et 3 N, 4 L, 28.67 mol, 2.00 equiv) was then added over 1 hour. The temperature was kept between 5 °C and 10 °C during the additions. The dark brown solution thus obtained was stirred for 12 hours at 20 °C and then chilled to 10 °C. With vigorous stirring, 18 L of saturated sodium bicarbonate solution and 36 L of water were sequentially added and the temperature was kept under 15 °C. The precipitation (filter cake) thus obtained was collected by filtration. The aqueous phase was then saturated with 12 kg of solid sodium chloride and extracted with EtOAc (2 x 18 L). The combined organic layer was washed with saturated sodium bicarbonate solution (18 L), and water (2 x 18 L) in sequence.
• the filter cake from the previous filtration was dissolved back in the organic phase.
• the dark brown solution thus obtained was washed twice with 18 L of water each and then concentrated under reduced pressure (40 - 50 °C, 30 mm Hg) to give 5.0 kg of the crude product as viscous brown oil.
• the crude product 17 obtained above was dissolved in EtOH (8.15 L) at 50 °C. Water (16.3 L) was added over 30 minutes. The brown solution was seeded, cooled to 20 °C over 3 hours with stirring and stirred at 20 °C for 12 h.
• the resulting reaction mixture was then warmed to room temperature and an additional amount of 6.0 N aqueous hydrochloric acid (HCl, 1050 mL, 6.30 mol, 21.0 equiv) was slowly introduced to the reaction mixture at room temperature in 8 hours via the addition funnel.
• the reaction mixture was then cooled to 0 °C before being treated with 30% aqueous sodium hydroxide (NaOH, 860 mL, 8.57 mmol, 28.6 equiv) while the internal temperature was kept at below 10 °C.
• the resulting reaction mixture was subsequently warmed to room temperature prior to addition of solid sodium bicarbonate (NaHC0 3 , 85.0 g, 1.01 mol, 3.37 equiv) in 1 hour.
• the crude product obtained above was dissolved in methyl teri-butyl ether (MTBE, 22 L) at 60 °C in a 50 L reactor equipped with a mechanic stirrer, a thermometer, an addition funnel and a septum. Hexanes (22 L) was added over 1 hour at 60 °C. The solution was then seeded, cooled to 20 °C over 3 hours and stirred at 20 °C for 12 hours. The precipitation was collected by filtration. The resultant cake was washed with a mixture of MTBE and hexane (1 : 15, 3 L) and dried in a vacuum oven for 10 hours at 50 °C to provide the compound 19 (6.83 kg, 13.42 mol, 94.0%) as a white powder.
• MTBE methyl teri-butyl ether
• the aqueous phase was extracted with dichloromethane (29 L).
• the combined organic phase was sequentially washed with 0.1 N aqueous hydrochloric acid solution (16 L), 4% aqueous sodium bicarbonate solution (16 L), 8% aqueous sodium chloride solution (2 x 16 L).
• the resultant organic phase was partially concentrated and filtered.
• the filtrate was subjected to solvent exchange by gradually adding acetonitrile (65 L) under vacuum.
• the white solids were collected by filtration, washed with acetonitrile (10 L) and dried at 40 - 50 °C in a vacuum oven with nitrogen purge to afford compound 21 (4.26 kg, 6.23 mol, 92.9%).
• reaction mixture After stirring at 20 ⁇ 5 °C for 18 hours, the reaction mixture was cooled to 0 - 5 °C and an additional amount of BF3 OE12 (0.34 mL, 2.70 mmol, 0.2 equiv) was introduced to the reaction mixture at below 5.0 °C. The resulting reaction mixture was warmed to 20 ⁇ 5 °C, and kept stirring at room temperature for an additional 5 hours. The reaction mixture was then cooled to 0 - 5 °C before water (12.17 mL, 0.676 mol, 50 equiv) was added. The internal temperature was kept at below 5.0 °C during addition of water. The resultant mixture was warmed to 20 ⁇ 5 °C and kept stirring at room temperature for 2 hours.
• BF3 OE12 0.34 mL, 2.70 mmol, 0.2 equiv
• reaction mixture was then cooled to 0 - 5 °C and aqueous ammonium hydroxide (NH4OH, 5 N, 121.7 mmol, 9.0 equiv) was added. During addition of aqueous ammonium hydroxide solution, the internal temperature was kept at below 5.0 °C. The resulting reaction mixture was warmed to 20 ⁇ 5 °C and stirred at room temperature for 20 hours. Once the SEM-deprotection was deemed complete, the reaction mixture was filtered, and the solids were washed with EtOAc (9.25 mL). The filtrates were combined and diluted with EtOAc (74 mL). The diluted organic solution was washed with 13% aqueous sodium chloride solution (46.2 mL).
• the organic phase was then diluted with EtOAc (55.5 mL) before being concentrated to a minimum volume under reduced pressure.
• EtOAc 120 mL was added to the residue, and the resulting solution was stirred at 20 ⁇ 5 °C for 30 minutes.
• the solution was then washed with 7% aqueous sodium bicarbonate solution (2 x 46 mL) and 13% aqueous sodium bicarbonate solution (46 mL).
• the resultant organic phase was diluted with EtOAc (46 mL) and treated with water (64 mL) at 50 ⁇ 5 °C for 30 minutes. The mixture was cooled to 20 ⁇ 5 °C and the two phases were separated.
• the resulting white turbid was heated to 55 - 60 °C to provide a clear solution.
• the resultant solution was filtered through an in-line filter to another 100 L reactor.
• Heptane (23.8 L) was filtered through an in-line filter to a separated 50 L reactor.
• the filtered heptane was then charged to the acetone solution in the 100 L reactor at a rate while the internal temperature was kept at 55 - 60 °C.
• the reaction mixture in the 100 L reactor was then cooled to 20 ⁇ 5 °C and stirred at 20 ⁇ 5 °C for 16 hours.
• the white precipitates were collected by filtration and the cake was washed with heptane (2 x 5.1 L) and dried on the filter under nitrogen with a pulling vacuum.
• the compound of Formula I herein was tested for inhibitory activity of JAK targets according to the following in vitro assay described in Park et ai, Analytical Biochemistry 1999, 269, 94-104.
• the catalytic domains of human JA 1 (a.a. 837- 1 142) and JAK2 (a.a. 828-1 132) with an N-terminal His tag were expressed using baculovirus in insect cells and purified.
• the catalytic activity of JAK1 and JAK.2 was assayed by measuring the phosphorylation of a biotinylated peptide.
• phosphorylated peptide was detected by homogenous time resolved fluorescence (HTRF).
• IC50S of compounds were measured for each kinase in the 40 microL reactions that contain the enzyme, ATP and 500 nM peptide in 50 mM Tris (pH 7.8) buffer with 100 mM NaCl, 5 mM DTT, and 0.1 mg/mL (0.01 %) BSA.
• ATP concentration in the reactions was 1 mM.
• Reactions were carried out at room temperature for 1 hr and then stopped with 20 ⁇ L ⁇ 45 mM EDTA, 300 nM SA-APC, 6 nM Eu-Py20 in assay buffer (Perkin Elmer, Boston, MA).
• Binding to the Europium labeled antibody took place for 40 minutes and HTRF signal was measured on a Fusion plate reader (Perkin Elmer, Boston, MA).
• the compound of Example 1 and the adipic acid salt had an IC50 at JA 1 of ⁇ 5 nM (measured at 1 mM ATP) with a JAK2/JAK1 ratio of > 10 (measured at 1 mM ATP).
• Cancer cell lines dependent on cytokines and hence JAK/STAT signal transduction, for growth can be plated at 6000 cells per well (96 well plate format) in RPMI 1640, 10% FBS, and 1 nG/mL of appropriate cytokine.
• Compounds can be added to the cells in DMSO/media (final concentration 0.2% DMSO) and incubated for 72 hours at 37 °C, 5% C0 2 .
• the effect of compound on cell viability is assessed using the CellTiter-Glo Luminescent Cell Viability Assay (Promega) followed by TopCount (Perkin Elmer, Boston, MA) quantitation. Potential off-target effects of compounds are measured in parallel using a non-JAK driven cell line with the same assay readout. All experiments are typically performed in duplicate.
• the above cell lines can also be used to examine the effects of compounds on phosphorylation of JA kinases or potential downstream substrates such as STAT proteins, Akt, Shp2, or Erk. These experiments can be performed following an overnight cytokine starvation, followed by a brief preincubation with compound (2 hours or less) and cytokine stimulation of approximately 1 hour or less. Proteins are then extracted from cells and analyzed by techniques familiar to those schooled in the art including Western blotting or ELISAs using antibodies that can differentiate between phosphorylated and total protein. These experiments can utilize normal or cancer cells to investigate the activity of compounds on tumor cell survival biology or on mediators of inflammatory disease.
• cytokines such as IL-6, IL-12, IL-23, or IFN can be used to stimulate JA activation resulting in phosphorylation of STAT protein(s) and potentially in transcriptional profiles (assessed by array or qPCR technology) or production and/or secretion of proteins, such as IL- 17.
• the ability of compounds to inhibit these cytokine mediated effects can be measured using techniques common to those schooled in the art.
• Endpoints include the effects of compounds on cell survival, proliferation, and phosphorylated JAK, STAT, Akt, or Erk proteins.
• PBMCs Peripheral blood mononuclear cells
• Freshly isolated human T-cells can be maintained in culture medium (RPMI 1640 supplemented with 10% fetal bovine serum, 100 U/ml penicillin, 100 ⁇ g/ml streptomycin) at a density of 2 x 10 6 cells/ml at 37 °C for up to 2 days.
• T-cells are first treated with Phytohemagglutinin (PHA) at a final concentration of 10 ⁇ g/mL for 72h. After washing once with PBS, 6000 cells/well are plated in 96-well plates and treated with compounds at different concentrations in the culture medium in the presence of 100 U/mL human IL-2 (ProSpec-Tany TechnoGene; Rehovot, Israel). The plates are incubated at 37 °C for 72h and the proliferation index is assessed using CellTiter-Glo Luminescent reagents following the manufactory suggested protocol (Promega; Madison, Wl).
• Example C In vivo anti-tumor efficacy
• Compounds herein can be evaluated in human tumor xenograft models in immune compromised mice.
• a tumorigenic variant of the ⁇ -6 plasmacytoma cell line can be used to inoculate SC1D mice subcutaneously (Burger, R., et al. Hematol J. 2:42-53, 2001 ).
• Tumor bearing animals can then be randomized into drug or vehicle treatment groups and different doses of compounds can be administered by any number of the usual routes including oral, i.p., or continuous infusion using implantable pumps. Tumor growth is followed over time using calipers.
• tumor samples can be harvested at any time after the initiation of treatment for analysis as described above (Example B) to evaluate compound effects on JA activity and downstream signaling pathways.
• selectivity of the compound(s) can be assessed using xenograft tumor models that are driven by other know kinases (e.g. Bcr-Abl) such as the K562 tumor model.
• Bcr-Abl know kina
• the murine skin contact delayed-type hypersensitivity (DTH) response is considered to be a valid model of clinical contact dermatitis, and other T-lymphocyte mediated immune disorders of the skin, such as psoriasis (Immunol Today. 1998 Jan; 19(l ):37-44).
• Murine DTH shares multiple characteristics with psoriasis, including the immune infiltrate, the accompanying increase in inflammatory cytokines, and keratinocyte hyperproliferation.
• many classes of agents that are efficacious in treating psoriasis in the clinic are also effective inhibitors of the DTH response in mice (Agents Actions. 1993 Jan;38( l -2): 1 16-21 ).
• mice are sensitized with a topical application, to their shaved abdomen with the antigen 2,4,dinitro-fluorobenzene (DNFB).
• DNFB 2,4,dinitro-fluorobenzene
• ears are measured for thickness using an engineer's micrometer. This measurement is recorded and used as a baseline.
• Both of the animals' ears are then challenged by a topical application of DNFB in a total of 20 (10 ⁇ L ⁇ on the internal pinna and 10 ⁇ - on the external pinna) at a concentration of 0.2%. Twenty-four to seventy-two hours after the challenge, ears are measured again.
• Treatment with the test compounds is given throughout the sensitization and challenge phases (day -1 to day 7) or prior to and throughout the challenge phase (usually afternoon of day 4 to day 7).
• Treatment of the test compounds (in different concentration) is administered either systemicaily or topically (topical application of the treatment to the ears). Efficacies of the test compounds are indicated by a reduction in ear swelling comparing to the situation without the treatment. Compounds causing a reduction of 20% or more were considered efficacious. In some experiments, the mice are challenged but not sensitized (negative control).
• the inhibitive effect (inhibiting activation of the JAK-STAT pathways) of the test compounds can be confirmed by immunohistochemical analysis.
• Activation of the JAK-STAT pathway(s) results in the formation and translocation of functional transcription factors.
• the influx of immune cells and the increased proliferation of keratinocytes should also provide unique expression profile changes in the ear that can be investigated and quantified.
• Formalin fixed and paraffin embedded ear sections (harvested after the challenge phase in the DTH model) are subjected to immunohistochemical analysis using an antibody that specifically interacts with phosphorylated STAT3 (clone 58E12, Cell Signaling Technologies).
• test compounds a clinically efficacious treatment for psoriasis
• dexamethasone a clinically efficacious treatment for psoriasis
• Test compounds and the dexamethasone can produce similar transcriptional changes both qualitatively and quantitatively, and both the test compounds and dexamethasone can reduce the number of infiltrating cells.
• Both systemicaily and topical administration of the test compounds can produce inhibitive effects, i.e., reduction in the number of infiltrating cells and inhibition of the transcriptional changes.
• Example E In vivo anti-inflammatory activity
• rodent models of arthritis can be used to evaluate the therapeutic potential of compounds dosed preventatively or therapeutically.
• These models include but are not limited to mouse or rat collagen-induced arthritis, rat adjuvant-induced arthritis, and collagen antibody- induced arthritis.
• Autoimmune diseases including, but not limited to, multiple sclerosis, type I-diabetes mellitus, uveoretinitis, thyroditis, myasthenia gravis, immunoglobulin nephropathies, myocarditis, airway sensitization (asthma), lupus, or colitis may also be used to evaluate the therapeutic potential of compounds herein.
• These models are well established in the research community and are familiar to those schooled in the art (Current Protocols in Immunology, Vol 3., Coligan, J.E. et al, Wiley Press.; Methods in Molecular Biology: Vol. 225, Inflammation Protocols., Winyard, P.G. and Willoughby, D.A., Humana Press, 2003.).
• Example F Animal Models for the Treatment of Dry Eye, Uveitis, and
• Agents may be evaluated in one or more preclinical models of dry eye known to those schooled in the art including, but not limited to, the rabbit concanavalin A (ConA) lacrimal gland model, the scopolamine mouse model (subcutaneous or transdermal), the Botulinumn mouse lacrimal gland model, or any of a number of spontaneous rodent auto-immune models that result in ocular gland dysfunction (e.g., the rabbit concanavalin A (ConA) lacrimal gland model, the scopolamine mouse model (subcutaneous or transdermal), the Botulinumn mouse lacrimal gland model, or any of a number of spontaneous rodent auto-immune models that result in ocular gland dysfunction (e.g.
• ConA rabbit concanavalin A
• scopolamine mouse model subcutaneous or transdermal
• Botulinumn mouse lacrimal gland model or any of a number of spontaneous rodent auto-immune models that result in ocular gland dysfunction
• NOD-SCID NOD-SCID, MRL/lpr, or NZB/NZW
• Barabino et al. Experimental Eye Research 2004, 79, 613-621 and Schrader et al., Developmental Opthalmology, Karger 2008, 41 , 298-312, each of which is incorporated herein by reference in its entirety).
• Endpoints in these models may include histopathology of the ocular glands and eye (cornea, etc.) and possibly the classic Schirmer test or modified versions thereof (Barabino et al.) which measure tear production. Activity may be assessed by dosing via multiple routes of administration (e.g. systemic or topical) which may begin prior to or after measurable disease exists.
• routes of administration e.g. systemic or topical
• Agents may be evaluated in one or more preclinical models of uveitis known to those schooled in the art. These include, but are not limited to, models of experimental autoimmune uveitis (EAU) and endotoxin induced uveitis (EIU). EAU experiements may be performed in the rabbit, rat, or mouse and may involve passive or activate immunization. For instance, any of a number or retinal antigens may be used to sensitize animals to a relevant immunogen after which animals may be challenged ocuarly with the same antigen.
• the EIU model is more acute and involves local or systemic administration of lipopolysaccaride at sublethal doses.
• Endpoints for both the EIU and EAU models may include fundoscopic exam, histopathology amongst others. These models are reviewed by Smith et al. (Immunology and Cell Biology 1998, 76, 497-512, which is incorporated herein by reference in its entirety). Activity is assessed by dosing via multiple routes of administration (e.g. systemic or topical) which may begin prior to or after measurable disease exists. Some models listed above may also develop scleritis/episcleritis, chorioditis, cyclitis, or ulceris and are therefore useful in investigating the potential activity of compounds for the therapeutic treatment of these diseases.
• routes of administration e.g. systemic or topical
• Agents may also be evaluated in one or more preclinical models of conjunctivitis known those schooled in the art. These include, but are not limited to, rodent models utilizing guinea-pig, rat, or mouse.
• the guinea-pig models include those utilizing active or passive immunization and/or immune challenge protocols with antigens such as ovalbumin or ragweed (reviewed in Groneberg, D.A., et al., Allergy 2003, 58, 1 101-1 1 13, which is incorporated herein by reference in its entirety).
• Rat and mouse models are similar in general design to those in the guinea- pig (also reviewed by Groneberg). Activity may be assessed by dosing via multiple routes of administration (e.g.
• Example G In vivo protection of bone
• Compounds may be evaluated in various preclinical models of osteopenia, osteoporosis, or bone resorption known to those schooled in the art.
• ovariectomized rodents may be used to evaluate the ability of compounds to affect signs and markers of bone remodeling and/or density (W.S.S. Jee and W. Yao, J Musculoskel. Nueron. Interact., 2001 , 1 (3), 193-207, which is incorporated herein by reference in its entirety).
• bone density and architecture may be evaluated in control or compound treated rodents in models of therapy (e.g. glucocorticoid) induced osteopenia (Yao, et al.

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