DE69515100D1 - Kovalente Kupplung von thiazole Orange an Oligonukleotide zum Nachweis von Nukleinsäuren - Google Patents

Kovalente Kupplung von thiazole Orange an Oligonukleotide zum Nachweis von Nukleinsäuren

Info

Publication number
DE69515100D1
DE69515100D1 DE69515100T DE69515100T DE69515100D1 DE 69515100 D1 DE69515100 D1 DE 69515100D1 DE 69515100 T DE69515100 T DE 69515100T DE 69515100 T DE69515100 T DE 69515100T DE 69515100 D1 DE69515100 D1 DE 69515100D1
Authority
DE
Germany
Prior art keywords
oligonucleotides
detection
nucleic acids
covalent coupling
thiazole orange
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69515100T
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English (en)
Other versions
DE69515100T2 (de
Inventor
C Preston Linn
J Bruce Pitner
Pat D Mize
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
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Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Publication of DE69515100D1 publication Critical patent/DE69515100D1/de
Application granted granted Critical
Publication of DE69515100T2 publication Critical patent/DE69515100T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
DE69515100T 1994-07-18 1995-07-18 Kovalente Kupplung von thiazole Orange an Oligonukleotide zum Nachweis von Nukleinsäuren Expired - Lifetime DE69515100T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/276,238 US5597696A (en) 1994-07-18 1994-07-18 Covalent cyanine dye oligonucleotide conjugates

Publications (2)

Publication Number Publication Date
DE69515100D1 true DE69515100D1 (de) 2000-03-23
DE69515100T2 DE69515100T2 (de) 2000-09-14

Family

ID=23055793

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69515100T Expired - Lifetime DE69515100T2 (de) 1994-07-18 1995-07-18 Kovalente Kupplung von thiazole Orange an Oligonukleotide zum Nachweis von Nukleinsäuren

Country Status (5)

Country Link
US (1) US5597696A (de)
EP (1) EP0710668B1 (de)
JP (1) JP2809340B2 (de)
CA (1) CA2152446C (de)
DE (1) DE69515100T2 (de)

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