DE69003104D1 - Verfahren zum nachweis von spezifischen sequenzen von nukleinsäuren und ihre verwendungen. - Google Patents

Verfahren zum nachweis von spezifischen sequenzen von nukleinsäuren und ihre verwendungen.

Info

Publication number
DE69003104D1
DE69003104D1 DE90909455T DE69003104T DE69003104D1 DE 69003104 D1 DE69003104 D1 DE 69003104D1 DE 90909455 T DE90909455 T DE 90909455T DE 69003104 T DE69003104 T DE 69003104T DE 69003104 D1 DE69003104 D1 DE 69003104D1
Authority
DE
Germany
Prior art keywords
nucleic acids
specific sequences
detecting specific
detecting
sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE90909455T
Other languages
English (en)
Other versions
DE69003104T2 (de
Inventor
Robert Teoule
Thierry Livache
Brigitte Fouque
Sylvie Sauvaigo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CIS Bio International SA
Original Assignee
CIS Bio International SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR8907710A external-priority patent/FR2648152A1/fr
Priority claimed from FR8910643A external-priority patent/FR2650839A1/fr
Application filed by CIS Bio International SA filed Critical CIS Bio International SA
Publication of DE69003104D1 publication Critical patent/DE69003104D1/de
Application granted granted Critical
Publication of DE69003104T2 publication Critical patent/DE69003104T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701Specific hybridization probes
    • C12Q1/708Specific hybridization probes for papilloma
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE90909455T 1989-06-12 1990-06-12 Verfahren zum nachweis von spezifischen sequenzen von nukleinsäuren und ihre verwendungen. Expired - Fee Related DE69003104T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR8907710A FR2648152A1 (fr) 1989-06-12 1989-06-12 Procede de detection et/ou d'identification d'acides nucleiques par amplification et hybridation en solution et ses applications
FR8910643A FR2650839A1 (fr) 1989-08-08 1989-08-08 Procede de detection en phase homogene liquide de sequences specifiques d'acides nucleiques et ses applications
PCT/FR1990/000410 WO1990015881A1 (fr) 1989-06-12 1990-06-12 Procede de detection de sequences specifiques d'acides nucleiques et ses applications

Publications (2)

Publication Number Publication Date
DE69003104D1 true DE69003104D1 (de) 1993-10-07
DE69003104T2 DE69003104T2 (de) 1994-03-17

Family

ID=26227389

Family Applications (1)

Application Number Title Priority Date Filing Date
DE90909455T Expired - Fee Related DE69003104T2 (de) 1989-06-12 1990-06-12 Verfahren zum nachweis von spezifischen sequenzen von nukleinsäuren und ihre verwendungen.

Country Status (7)

Country Link
US (1) US5514540A (de)
EP (1) EP0478630B1 (de)
JP (1) JPH04506599A (de)
AU (1) AU5838690A (de)
DE (1) DE69003104T2 (de)
ES (1) ES2045932T3 (de)
WO (1) WO1990015881A1 (de)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0491790A (ja) * 1990-08-02 1992-03-25 Shionogi & Co Ltd 2段階pcr法
DE69128520T2 (de) * 1990-10-31 1998-07-09 Tosoh Corp Verfahren zum Nachweis oder Quantifizierung von Zielnukleinsäuren
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
EP0530009B1 (de) * 1991-08-27 1999-01-13 Zeneca Limited Verfahren zur Charakterisierung genomischer DNA
US5853989A (en) * 1991-08-27 1998-12-29 Zeneca Limited Method of characterisation of genomic DNA
DE4234086A1 (de) * 1992-02-05 1993-08-12 Diagen Inst Molekularbio Verfahren zur bestimmung von in vitro amplifizierten nukleinsaeuresequenzen
US5340728A (en) * 1992-12-09 1994-08-23 E. I. Du Pont De Nemours And Company Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction
US5547861A (en) * 1994-04-18 1996-08-20 Becton, Dickinson And Company Detection of nucleic acid amplification
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
WO1999034014A2 (en) * 1997-12-23 1999-07-08 Roche Diagnostics Gmbh A method for the determination of a nucleic acid
US6565727B1 (en) 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
US6716578B1 (en) 1999-03-08 2004-04-06 Bioforce Nanosciences, Inc. Method for solid state genome analysis
US6531302B1 (en) * 1999-04-12 2003-03-11 Nanogen/Becton Dickinson Partnership Anchored strand displacement amplification on an electronically addressable microchip
US6326173B1 (en) * 1999-04-12 2001-12-04 Nanogen/Becton Dickinson Partnership Electronically mediated nucleic acid amplification in NASBA
US6238868B1 (en) * 1999-04-12 2001-05-29 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification and bioelectronic chip technology
EP1177423A4 (de) * 1999-04-12 2004-10-27 Nanogen Becton Dickinson Partn Verstärkung und trennung von nukleinsäuresequenzen mittels strangverschiebeverstärkung und bioelektronischer
US6573369B2 (en) * 1999-05-21 2003-06-03 Bioforce Nanosciences, Inc. Method and apparatus for solid state molecular analysis
US20030186311A1 (en) * 1999-05-21 2003-10-02 Bioforce Nanosciences, Inc. Parallel analysis of molecular interactions
US20020042081A1 (en) * 2000-10-10 2002-04-11 Eric Henderson Evaluating binding affinities by force stratification and force panning
US20030073250A1 (en) * 1999-05-21 2003-04-17 Eric Henderson Method and apparatus for solid state molecular analysis
US6897015B2 (en) * 2000-03-07 2005-05-24 Bioforce Nanosciences, Inc. Device and method of use for detection and characterization of pathogens and biological materials
ATE402760T1 (de) * 2000-08-15 2008-08-15 Bioforce Nanosciences Inc Vorrichtung zur bildung von nanomolekularen netzwerken
WO2003005022A1 (en) * 2001-07-03 2003-01-16 Bioforce Nanosciences Inc A method for solid state genome analysis
WO2003008941A2 (en) * 2001-07-17 2003-01-30 Bioforce Nanosciences, Inc. Combined molecular blinding detection through force microscopy and mass spectrometry
US7042488B2 (en) 2001-09-27 2006-05-09 Fujinon Corporation Electronic endoscope for highlighting blood vessel
US20050239193A1 (en) * 2002-05-30 2005-10-27 Bioforce Nanosciences, Inc. Device and method of use for detection and characterization of microorganisms and microparticles
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
US7329545B2 (en) 2002-09-24 2008-02-12 Duke University Methods for sampling a liquid flow
KR20050100367A (ko) * 2003-01-02 2005-10-18 바이오포스 나노사이언스, 인크. 소용적 샘플 내의 분자 분석용 방법 및 장치
DE10346286B3 (de) 2003-10-06 2005-04-14 J. Eberspächer GmbH & Co. KG Abgasreinigungsanordnung
ATE390562T1 (de) * 2004-11-19 2008-04-15 Ebm Papst St Georgen Gmbh & Co Anordnung mit einem luefter und einer pumpe
WO2006081558A2 (en) 2005-01-28 2006-08-03 Duke University Apparatuses and methods for manipulating droplets on a printed circuit board

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4257774A (en) * 1979-07-16 1981-03-24 Meloy Laboratories, Inc. Intercalation inhibition assay for compounds that interact with DNA or RNA
EP0119209A1 (de) * 1982-09-20 1984-09-26 President And Fellows Of Harvard College Identifizierung von mikroorganismen
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4824776A (en) * 1985-07-25 1989-04-25 Molecular Biosystems, Inc. Method for increasing the sensitivity of nucleic acid hybridization assays
JP3293820B2 (ja) * 1985-12-13 2002-06-17 エンゾ− バイオケム インコ−ポレイテツド 標的ポリヌクレオチドにハイブリツド形成するための新規な一工程方法とポリヌクレオチド化合物
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
AU622104B2 (en) * 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore
CA1317535C (en) * 1987-06-30 1993-05-11 Nanibhushan Dattagupta Assay of sequences using amplified genes
CA1340843C (en) * 1987-07-31 1999-12-07 J. Lawrence Burg Selective amplification of target polynucleotide sequences
AU2735988A (en) * 1987-12-21 1989-07-13 Amoco Corporation Target and background capture methods with amplification for affinity assays

Also Published As

Publication number Publication date
EP0478630A1 (de) 1992-04-08
US5514540A (en) 1996-05-07
WO1990015881A1 (fr) 1990-12-27
ES2045932T3 (es) 1994-01-16
JPH04506599A (ja) 1992-11-19
EP0478630B1 (de) 1993-09-01
DE69003104T2 (de) 1994-03-17
AU5838690A (en) 1991-01-08

Similar Documents

Publication Publication Date Title
DE69003104D1 (de) Verfahren zum nachweis von spezifischen sequenzen von nukleinsäuren und ihre verwendungen.
ATA64388A (de) Verbessertes verfahren zum nachweis von nucleinsaeuren und reagenzienkombination und besteck zu seiner durchfuehrung
DE69520330D1 (de) Verfahren zum Nachweis von spezifischen Nukleinsäuresequenzen
DE68918657D1 (de) Verfahren zum Nachweis spezifischer Nukleinsäuresequenzen.
DE68915202D1 (de) Verfahren zur Vermehrung und zum Nachweis von Nukleinsäuresequenzen.
DE58903898D1 (de) Verfahren zum nachweis von nukleinsaeuren.
DE69128520D1 (de) Verfahren zum Nachweis oder Quantifizierung von Zielnukleinsäuren
DE3789905D1 (de) Verfahren zum Nachweis von elektrophoretisch getrennten Oligonukleotiden.
DE3689412D1 (de) Verfahren zur durchführung von hybridisierungstests von nukleinsäuren.
DE68907407D1 (de) Verfahren zur sequenzanalyse von dna.
DE69207580D1 (de) Verfahren zum DNS-Nachweis
DE69125441D1 (de) Verfahren zum Gennachweis
DE69017923D1 (de) Verfahren und Einrichtung zum Detektieren von nach aussen gerichteten Signalen.
DE3886683D1 (de) Verfahren und Vorrichtung zum Erfassen von Tinten.
DE58908694D1 (de) Oligonucleotidbank und verfahren zur dna-sequenzierung.
DE3575621D1 (de) Verfahren und anordnung zum kennzeichnen und erkennen von gegenstaenden.
DE69033850D1 (de) Nukleinsäuresonden zum Nachweis von HPV-Transkripts
DE69434255D1 (de) Nukleinsäuresonde zum nachweis von lactobacillus
DE69029454D1 (de) Verfahren zum Aufweiten von Bohrungsendbereichen von Kapillarröhrchen
ATE96523T1 (de) Verfahren zum schnellen und dichten verbinden von rohren.
DE3888653D1 (de) Verfahren und Testsatz zum Nachweis einer Nukleinsäure-Sequenz.
DE69015286D1 (de) Verfahren und Gerät zum Nachweisen von Fremdkörpern.
DE69000307D1 (de) Verfahren und anlage zur feststellung des spezifischen widerstandes von geologischen formationen.
DE3856485D1 (de) Verfahren zum Vergleichen von Fingerabdrücken
DE69222710D1 (de) Verfahren zum Nachweis von DNS-Basen mit Verwendung von Fluoreszenz

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee