CA2585235C - Docosahexaenoic acid producing strains of yarrowia lipolytica - Google Patents

Docosahexaenoic acid producing strains of yarrowia lipolytica Download PDF

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Publication number
CA2585235C
CA2585235C CA2585235A CA2585235A CA2585235C CA 2585235 C CA2585235 C CA 2585235C CA 2585235 A CA2585235 A CA 2585235A CA 2585235 A CA2585235 A CA 2585235A CA 2585235 C CA2585235 C CA 2585235C
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Prior art keywords
desaturase
seq
elongase
gene
host cell
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Expired - Fee Related
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CA2585235A
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English (en)
French (fr)
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CA2585235A1 (en
Inventor
Howard G. Damude
Peter John Gillies
Daniel Joseph Macool
Stephen K. Picataggio
James John Ragghianti
Zhixiong Xue
Narendra S. Yadav
Hongxiang Zhang
Quinn Qun Zhu
John E. Seip
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EIDP Inc
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EI Du Pont de Nemours and Co
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Publication of CA2585235C publication Critical patent/CA2585235C/en
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    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS OR COOKING OILS
    • A23D9/00—Other edible oils or fats, e.g. shortenings or cooking oils
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    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
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    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/115—Fatty acids or derivatives thereof; Fats or oils
    • A23L33/12—Fatty acids or derivatives thereof
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    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
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    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
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    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
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CA2585235A 2004-11-04 2005-11-03 Docosahexaenoic acid producing strains of yarrowia lipolytica Expired - Fee Related CA2585235C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US62481204P 2004-11-04 2004-11-04
US60/624,812 2004-11-04
PCT/US2005/040256 WO2006052871A2 (en) 2004-11-04 2005-11-03 Docosahexaenoic acid producing strains of yarrowia lipolytica

Publications (2)

Publication Number Publication Date
CA2585235A1 CA2585235A1 (en) 2006-05-18
CA2585235C true CA2585235C (en) 2014-09-16

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Family Applications (3)

Application Number Title Priority Date Filing Date
CA2585235A Expired - Fee Related CA2585235C (en) 2004-11-04 2005-11-03 Docosahexaenoic acid producing strains of yarrowia lipolytica
CA2585178A Expired - Fee Related CA2585178C (en) 2004-11-04 2005-11-03 High arachidonic acid producing strains of yarrowia lipolytica
CA2584719A Expired - Fee Related CA2584719C (en) 2004-11-04 2005-11-03 High eicosapentaenoic acid producing strains of yarrowia lipolytica

Family Applications After (2)

Application Number Title Priority Date Filing Date
CA2585178A Expired - Fee Related CA2585178C (en) 2004-11-04 2005-11-03 High arachidonic acid producing strains of yarrowia lipolytica
CA2584719A Expired - Fee Related CA2584719C (en) 2004-11-04 2005-11-03 High eicosapentaenoic acid producing strains of yarrowia lipolytica

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Country Link
US (8) US7550286B2 (enExample)
EP (5) EP1809756B1 (enExample)
JP (3) JP5139806B2 (enExample)
KR (3) KR20070085649A (enExample)
CN (4) CN101111601B (enExample)
CA (3) CA2585235C (enExample)
DK (4) DK1809756T3 (enExample)
NO (3) NO20072517L (enExample)
WO (3) WO2006055322A2 (enExample)

Families Citing this family (173)

* Cited by examiner, † Cited by third party
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US8313911B2 (en) * 2003-05-07 2012-11-20 E I Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US11952581B2 (en) 2003-08-01 2024-04-09 Basf Plant Science Gmbh Process for the production of polyunsaturated fatty acids in transgenic organisms
PT2172536T (pt) 2003-08-01 2016-08-17 Basf Plant Science Gmbh Processo para a produção de ácidos gordos poli-insaturados em organismos transgénicos
CN1930277B (zh) 2004-02-27 2011-02-02 巴斯福植物科学有限公司 在转基因植物中产生多不饱和脂肪酸的方法
PL1756280T3 (pl) 2004-04-22 2015-04-30 Commw Scient Ind Res Org Synteza długołańcuchowych wielonienasyconych kwasów tłuszczowych w komórkach rekombinowanych
ES2529572T3 (es) 2004-04-22 2015-02-23 Commonwealth Scientific And Industrial Research Organisation Síntesis de ácidos grasos poliinsaturados de cadena larga por células recombinantes
AU2005250074B2 (en) * 2004-06-04 2011-04-14 Fluxome Sciences A/S Metabolically engineered cells for the production of polyunsaturated fatty acids
US7550286B2 (en) 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
US20060094102A1 (en) * 2004-11-04 2006-05-04 Zhixiong Xue Ammonium transporter promoter for gene expression in oleaginous yeast
US7879591B2 (en) 2004-11-04 2011-02-01 E.I. Du Pont De Nemours And Company High eicosapentaenoic acid producing strains of Yarrowia lipolytica
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