BR112013004351A2 - célula hospedeira microbiana recombinante e método para a produção de um óleo microbiano compreendendo ácido eicosapentaenócio. - Google Patents

célula hospedeira microbiana recombinante e método para a produção de um óleo microbiano compreendendo ácido eicosapentaenócio.

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Publication number
BR112013004351A2
BR112013004351A2 BR112013004351A BR112013004351A BR112013004351A2 BR 112013004351 A2 BR112013004351 A2 BR 112013004351A2 BR 112013004351 A BR112013004351 A BR 112013004351A BR 112013004351 A BR112013004351 A BR 112013004351A BR 112013004351 A2 BR112013004351 A2 BR 112013004351A2
Authority
BR
Brazil
Prior art keywords
producing
acid
microbial
eicosapentaenate
host cell
Prior art date
Application number
BR112013004351A
Other languages
English (en)
Inventor
Hongxiang Zhang
Narendra S Yadav
Pamela L Sharpe
Quinn Qun Zhu
Seung-Pyo Hong
Zhixiong Xue
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of BR112013004351A2 publication Critical patent/BR112013004351A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

célula hospedeiramicrfobiana recombinante e método para a produção de um óleo microbiano compreendendo ácido eicosapentaenóico. são divulgadas na presente invenção cepas geneticamente manipuladas da levedura oleaginosa yarrowia lipolytica que são capazes de produzir óleo microbiano compreendendo mais do que 25 por cento em peso de ácido eicosapentaenóico ["epa'], um ácido graxo poli-insaturado ômega-3,mensurado como uma percentagem em peso celular seco.
BR112013004351A 2010-08-26 2011-08-26 célula hospedeira microbiana recombinante e método para a produção de um óleo microbiano compreendendo ácido eicosapentaenócio. BR112013004351A2 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US37724810P 2010-08-26 2010-08-26
US201061428277P 2010-12-30 2010-12-30
US201161479921P 2011-04-28 2011-04-28
PCT/US2011/049384 WO2012027689A1 (en) 2010-08-26 2011-08-26 Recombinant microbial host cells for high eicosapentaenoic acid production

Publications (1)

Publication Number Publication Date
BR112013004351A2 true BR112013004351A2 (pt) 2016-05-31

Family

ID=45697764

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013004351A BR112013004351A2 (pt) 2010-08-26 2011-08-26 célula hospedeira microbiana recombinante e método para a produção de um óleo microbiano compreendendo ácido eicosapentaenócio.

Country Status (11)

Country Link
US (1) US8703473B2 (pt)
EP (1) EP2609203B1 (pt)
JP (1) JP2013535987A (pt)
KR (1) KR20130138760A (pt)
CN (1) CN103249834B (pt)
AU (1) AU2011293180B2 (pt)
BR (1) BR112013004351A2 (pt)
CA (1) CA2807834C (pt)
CL (1) CL2013000533A1 (pt)
DK (1) DK2609203T3 (pt)
WO (1) WO2012027689A1 (pt)

Families Citing this family (15)

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EP2609202A1 (en) * 2010-08-26 2013-07-03 E.I. Du Pont De Nemours And Company Mutant hpgg motif and hdash motif delta-5 desaturases and their use in making polyunsaturated fatty acids
WO2012135773A1 (en) * 2011-03-31 2012-10-04 E. I. Du Pont De Nemours And Company Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast
US8735094B2 (en) 2011-04-05 2014-05-27 E I Du Pont De Nemours And Company Yarrowia n-alkane-hydroxylating cytochrome P450 promoter regions for gene expression in yeast
JP2014516537A (ja) 2011-05-26 2014-07-17 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 組換え油性微生物における、その中のオイル含量を増加させるためのカレオシンの発現
JP6351582B2 (ja) 2012-06-19 2018-07-04 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ アシルＣｏＡ：リゾホスファチジルコリンアシルトランスフェラーゼおよびリン脂質：ジアシルグリセロールアシルトランスフェラーゼの同時発現による多価不飽和脂肪酸の産生の改善
DK2935601T3 (en) 2012-12-21 2018-06-18 Du Pont RECOMBINANT MICROBELL CELLS PRODUCING AT LEAST 28% EICOSAPENTAIC ACID AS DRY WEIGHT
JP2016502851A (ja) 2012-12-21 2016-02-01 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 微生物細胞中における脂質生成を改変するためのｓｏｕ２ソルビトール利用タンパク質をコードするポリヌクレオチドの下方制御
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WO2019209241A1 (en) 2018-04-23 2019-10-31 Dupont Nutrition Biosciences Aps Increasing export of 2' fucosyllactose from microbial cells through the expression of a heterologous nucleic acid
WO2020082221A1 (zh) * 2018-10-23 2020-04-30 利时雨 重组磷酸胆碱胞苷转移酶的粘红酵母携带外源多肽的活细胞脂质体及其应用
CN116555054B (zh) * 2023-06-09 2024-05-14 陕西海斯夫生物工程有限公司 一株高产dha的重组裂殖壶菌、其构建方法及应用

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Also Published As

Publication number Publication date
US8703473B2 (en) 2014-04-22
DK2609203T3 (da) 2018-10-01
AU2011293180A1 (en) 2013-01-24
CL2013000533A1 (es) 2013-10-18
CN103249834A (zh) 2013-08-14
CA2807834C (en) 2019-06-25
JP2013535987A (ja) 2013-09-19
CA2807834A1 (en) 2012-03-01
EP2609203A1 (en) 2013-07-03
EP2609203B1 (en) 2018-06-20
KR20130138760A (ko) 2013-12-19
WO2012027689A1 (en) 2012-03-01
CN103249834B (zh) 2016-10-26
AU2011293180B2 (en) 2017-03-02
US20120052537A1 (en) 2012-03-01

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