CL2013000533A1 - Celula huesped microbiana recombinante que produce aceite que comprende 25% en peso de acido eicosapentaenoico; celula que comprende una multienzima que comprende un polipeptido con al menos una delta-9 elongasa unida a al menos una delta-8 desaturasa metodo para elaborar aceite microbiano. - Google Patents

Celula huesped microbiana recombinante que produce aceite que comprende 25% en peso de acido eicosapentaenoico; celula que comprende una multienzima que comprende un polipeptido con al menos una delta-9 elongasa unida a al menos una delta-8 desaturasa metodo para elaborar aceite microbiano.

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Publication number
CL2013000533A1
CL2013000533A1 CL2013000533A CL2013000533A CL2013000533A1 CL 2013000533 A1 CL2013000533 A1 CL 2013000533A1 CL 2013000533 A CL2013000533 A CL 2013000533A CL 2013000533 A CL2013000533 A CL 2013000533A CL 2013000533 A1 CL2013000533 A1 CL 2013000533A1
Authority
CL
Chile
Prior art keywords
delta
oil
cell
multienzyme
elongase
Prior art date
Application number
CL2013000533A
Other languages
English (en)
Inventor
Qun Zhu
Seung-Pyo Hong
Pamela L Sharpe
Narendra S Yadav
Hongxiang Zhang
Zhix-Iong Xue
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of CL2013000533A1 publication Critical patent/CL2013000533A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CL2013000533A 2010-08-26 2013-02-25 Celula huesped microbiana recombinante que produce aceite que comprende 25% en peso de acido eicosapentaenoico; celula que comprende una multienzima que comprende un polipeptido con al menos una delta-9 elongasa unida a al menos una delta-8 desaturasa metodo para elaborar aceite microbiano. CL2013000533A1 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US37724810P 2010-08-26 2010-08-26
US201061428277P 2010-12-30 2010-12-30
US201161479921P 2011-04-28 2011-04-28

Publications (1)

Publication Number Publication Date
CL2013000533A1 true CL2013000533A1 (es) 2013-10-18

Family

ID=45697764

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2013000533A CL2013000533A1 (es) 2010-08-26 2013-02-25 Celula huesped microbiana recombinante que produce aceite que comprende 25% en peso de acido eicosapentaenoico; celula que comprende una multienzima que comprende un polipeptido con al menos una delta-9 elongasa unida a al menos una delta-8 desaturasa metodo para elaborar aceite microbiano.

Country Status (11)

Country Link
US (1) US8703473B2 (es)
EP (1) EP2609203B1 (es)
JP (1) JP2013535987A (es)
KR (1) KR20130138760A (es)
CN (1) CN103249834B (es)
AU (1) AU2011293180B2 (es)
BR (1) BR112013004351A2 (es)
CA (1) CA2807834C (es)
CL (1) CL2013000533A1 (es)
DK (1) DK2609203T3 (es)
WO (1) WO2012027689A1 (es)

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CA2833400A1 (en) 2011-05-26 2012-11-29 E.I. Dupont De Nemours And Company Expression of caleosin in recombinant oleaginous microorganisms to increase oil content therein
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DK2935601T3 (en) 2012-12-21 2018-06-18 Du Pont RECOMBINANT MICROBELL CELLS PRODUCING AT LEAST 28% EICOSAPENTAIC ACID AS DRY WEIGHT
CA2892516A1 (en) 2012-12-21 2014-06-26 E. I. Du Pont De Nemours And Company Down-regulation of a polynucleotide encoding a sou2 sorbitol utilization protein to modify lipid production in microbial cells
US9074160B2 (en) * 2013-03-07 2015-07-07 Algisys, Llc Production of omega-3 fatty acids from pythium species
US10513711B2 (en) 2014-08-13 2019-12-24 Dupont Us Holding, Llc Genetic targeting in non-conventional yeast using an RNA-guided endonuclease
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WO2019209241A1 (en) 2018-04-23 2019-10-31 Dupont Nutrition Biosciences Aps Increasing export of 2' fucosyllactose from microbial cells through the expression of a heterologous nucleic acid
WO2020082221A1 (zh) * 2018-10-23 2020-04-30 利时雨 重组磷酸胆碱胞苷转移酶的粘红酵母携带外源多肽的活细胞脂质体及其应用
CN116555054B (zh) * 2023-06-09 2024-05-14 陕西海斯夫生物工程有限公司 一株高产dha的重组裂殖壶菌、其构建方法及应用

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Also Published As

Publication number Publication date
CN103249834A (zh) 2013-08-14
BR112013004351A2 (pt) 2016-05-31
US20120052537A1 (en) 2012-03-01
AU2011293180B2 (en) 2017-03-02
DK2609203T3 (da) 2018-10-01
CA2807834A1 (en) 2012-03-01
JP2013535987A (ja) 2013-09-19
AU2011293180A1 (en) 2013-01-24
WO2012027689A1 (en) 2012-03-01
EP2609203A1 (en) 2013-07-03
CN103249834B (zh) 2016-10-26
EP2609203B1 (en) 2018-06-20
KR20130138760A (ko) 2013-12-19
CA2807834C (en) 2019-06-25
US8703473B2 (en) 2014-04-22

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