CL2011003161A1 - Celula huesped microbiana oleaginosa recombinante para la produccion mejorada de acido eicosapentaenoico, la que codifica un polipeptido con actividad acil coa lisofosfolipido, metodo para elaborar aceite que comprende dicho acido graso de cadena larga. - Google Patents

Celula huesped microbiana oleaginosa recombinante para la produccion mejorada de acido eicosapentaenoico, la que codifica un polipeptido con actividad acil coa lisofosfolipido, metodo para elaborar aceite que comprende dicho acido graso de cadena larga.

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Publication number
CL2011003161A1
CL2011003161A1 CL2011003161A CL2011003161A CL2011003161A1 CL 2011003161 A1 CL2011003161 A1 CL 2011003161A1 CL 2011003161 A CL2011003161 A CL 2011003161A CL 2011003161 A CL2011003161 A CL 2011003161A CL 2011003161 A1 CL2011003161 A1 CL 2011003161A1
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CL
Chile
Prior art keywords
polypeptide
activity
host cell
encodes
microbial host
Prior art date
Application number
CL2011003161A
Other languages
English (en)
Inventor
Narendra S Yadav
Hongxiang Zhu Qun Zhang
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Du Pont filed Critical Du Pont
Publication of CL2011003161A1 publication Critical patent/CL2011003161A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6481—Phosphoglycerides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6431—Linoleic acids [18:2[n-6]]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fats And Perfumes (AREA)

Abstract

Célula huésped microbiana oleaginosa recombinante para la producción mejorada de al menos un ácido graso poliinsaturado de cadena larga que codifica un polipéptido con actividad acilcoa: lisofosfolípido; método para elaborar aceite que comprende ácido eicosapentaenoico y/o ácido docosahexaenoico.
CL2011003161A 2009-06-16 2011-12-15 Celula huesped microbiana oleaginosa recombinante para la produccion mejorada de acido eicosapentaenoico, la que codifica un polipeptido con actividad acil coa lisofosfolipido, metodo para elaborar aceite que comprende dicho acido graso de cadena larga. CL2011003161A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US18735909P 2009-06-16 2009-06-16

Publications (1)

Publication Number Publication Date
CL2011003161A1 true CL2011003161A1 (es) 2012-05-25

Family

ID=43356702

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2011003161A CL2011003161A1 (es) 2009-06-16 2011-12-15 Celula huesped microbiana oleaginosa recombinante para la produccion mejorada de acido eicosapentaenoico, la que codifica un polipeptido con actividad acil coa lisofosfolipido, metodo para elaborar aceite que comprende dicho acido graso de cadena larga.

Country Status (10)

Country Link
EP (1) EP2443248B1 (es)
JP (1) JP5931721B2 (es)
CN (1) CN102459627B (es)
AU (1) AU2010260227B2 (es)
BR (1) BRPI1009690A2 (es)
CA (1) CA2763424C (es)
CL (1) CL2011003161A1 (es)
DK (1) DK2443248T3 (es)
NO (1) NO2443248T3 (es)
WO (1) WO2010147900A1 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2765911C (en) 2009-06-16 2018-12-18 E.I. Du Pont De Nemours And Company High eicosapentaenoic acid oils from improved optimized strains of yarrowia lipolytica
DK3384902T3 (da) 2012-02-17 2021-01-18 Alcresta Therapeutics Inc Fremgangsmåder, sammensætninger og indretninger til opfyldelse af diætmæssige behov for fedtsyre
EP2861059B1 (en) 2012-06-15 2017-05-03 Commonwealth Scientific and Industrial Research Organisation Production of long chain polyunsaturated fatty acids in plant cells
DK2861728T3 (en) * 2012-06-19 2018-03-26 Du Pont IMPROVED PREPARATION OF POLYUM SATURATED FAT ACIDS BY COEX EXPRESSION OF ACYL-COA: LYSOPHOSPHATIDYLCHOLINACYL TRANSFERASES AND PHOSPHOLIPID: DIACYLGLYCEROLACYL TRANSFERASES
NZ721036A (en) 2013-12-18 2023-07-28 Grains Res & Dev Corp Lipid comprising long chain polyunsaturated fatty acids
CA3228580A1 (en) * 2014-06-27 2015-12-30 Commonwealth Scientific And Industrial Research Organisation Lipid comprising docosapentaenoic acid
CN105219789B (zh) * 2014-06-27 2023-04-07 联邦科学技术研究组织 包含二十二碳五烯酸的提取的植物脂质
EA037184B1 (ru) * 2014-06-27 2021-02-16 Коммонвелт Сайнтифик Энд Индастриэл Рисерч Организэйшн Липид, содержащий докозапентаеновую кислоту
US10258590B2 (en) 2015-10-14 2019-04-16 Alcresta Therapeutics, Inc. Enteral feeding device and related methods of use
JP7097014B2 (ja) * 2018-02-13 2022-07-07 国立大学法人富山大学 藻類及びその使用
EP3586640A1 (en) 2018-06-21 2020-01-01 Nuseed Pty Ltd Dha enriched polyunsaturated fatty acid compositions
CN116669727A (zh) * 2020-08-11 2023-08-29 唯久生物有限公司 作为lpa受体拮抗剂的三唑-吡啶基取代的氮杂环己基乙酸化合物

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Also Published As

Publication number Publication date
NO2443248T3 (es) 2018-05-26
BRPI1009690A2 (pt) 2015-08-25
JP5931721B2 (ja) 2016-06-08
EP2443248A4 (en) 2013-07-10
CA2763424A1 (en) 2010-12-23
DK2443248T3 (en) 2018-03-12
CN102459627B (zh) 2015-07-29
JP2012529912A (ja) 2012-11-29
CA2763424C (en) 2018-06-12
AU2010260227B2 (en) 2015-01-29
EP2443248A1 (en) 2012-04-25
CN102459627A (zh) 2012-05-16
EP2443248B1 (en) 2017-12-27
AU2010260227A1 (en) 2011-11-10
WO2010147900A1 (en) 2010-12-23

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