BR112015023192A8 - Tioesterases e células para a produção de óleos personalizados - Google Patents

Tioesterases e células para a produção de óleos personalizados

Info

Publication number
BR112015023192A8
BR112015023192A8 BR112015023192A BR112015023192A BR112015023192A8 BR 112015023192 A8 BR112015023192 A8 BR 112015023192A8 BR 112015023192 A BR112015023192 A BR 112015023192A BR 112015023192 A BR112015023192 A BR 112015023192A BR 112015023192 A8 BR112015023192 A8 BR 112015023192A8
Authority
BR
Brazil
Prior art keywords
genes
cells
thioesterases
personalized
oils
Prior art date
Application number
BR112015023192A
Other languages
English (en)
Other versions
BR112015023192A2 (pt
Inventor
N Rudenko George
Casolari Jason
Franklin Scott
Original Assignee
Solazyme Inc
Terravia Holdings Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/837,996 external-priority patent/US9290749B2/en
Application filed by Solazyme Inc, Terravia Holdings Inc filed Critical Solazyme Inc
Publication of BR112015023192A2 publication Critical patent/BR112015023192A2/pt
Publication of BR112015023192A8 publication Critical patent/BR112015023192A8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • C12Y301/02014—Oleoyl-[acyl-carrier-protein] hydrolase (3.1.2.14), i.e. ACP-thioesterase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Edible Oils And Fats (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Fats And Perfumes (AREA)

Abstract

TIOESTERASES E CÉLULAS PARA A PRODUÇÃO DE ÓLEOS PERSONALIZADOS. A presente invenção apresenta genes acil-ACP tioesterase de planta da classe FatB e proteínas codificadas por estes genes. Os genes são úteis para construir células hospedeiras recombinantes tendo perfis alterados de ácidos graxos. São reveladas células hospedeiras de microalgas oleaginosas com os novos genes ou genes FatB previamente identificados. As células de microalgas produzem triglicerídeos com perfis de ácidos graxos úteis.
BR112015023192A 2013-03-15 2014-03-13 Tioesterases e células para a produção de óleos personalizados BR112015023192A8 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201361791861P 2013-03-15 2013-03-15
US13/837,996 US9290749B2 (en) 2013-03-15 2013-03-15 Thioesterases and cells for production of tailored oils
US201361917217P 2013-12-17 2013-12-17
PCT/US2014/026644 WO2014151904A1 (en) 2013-03-15 2014-03-13 Thioesterases and cells for production of tailored oils

Publications (2)

Publication Number Publication Date
BR112015023192A2 BR112015023192A2 (pt) 2017-11-21
BR112015023192A8 true BR112015023192A8 (pt) 2018-01-02

Family

ID=51581068

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015023192A BR112015023192A8 (pt) 2013-03-15 2014-03-13 Tioesterases e células para a produção de óleos personalizados

Country Status (9)

Country Link
EP (1) EP2971024A4 (pt)
JP (1) JP2016518112A (pt)
KR (1) KR20150128770A (pt)
CN (1) CN105143458A (pt)
AU (2) AU2014236763B2 (pt)
BR (1) BR112015023192A8 (pt)
CA (1) CA2904395A1 (pt)
MX (1) MX2015011507A (pt)
WO (1) WO2014151904A1 (pt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9567615B2 (en) 2013-01-29 2017-02-14 Terravia Holdings, Inc. Variant thioesterases and methods of use
US9816079B2 (en) 2013-01-29 2017-11-14 Terravia Holdings, Inc. Variant thioesterases and methods of use
US9783836B2 (en) 2013-03-15 2017-10-10 Terravia Holdings, Inc. Thioesterases and cells for production of tailored oils
US9290749B2 (en) 2013-03-15 2016-03-22 Solazyme, Inc. Thioesterases and cells for production of tailored oils
US9765368B2 (en) 2014-07-24 2017-09-19 Terravia Holdings, Inc. Variant thioesterases and methods of use
CN107208103A (zh) * 2014-09-18 2017-09-26 泰拉瑞亚控股公司 酰基‑acp硫酯酶及其突变体
US20180142218A1 (en) 2016-10-05 2018-05-24 Terravia Holdings, Inc. Novel acyltransferases, variant thioesterases, and uses thereof
US20230143841A1 (en) 2020-01-16 2023-05-11 Corbion Biotech, Inc. Beta-ketoacyl-acp synthase iv variants

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5344771A (en) 1990-04-26 1994-09-06 Calgene, Inc. Plant thiosterases
US5298421A (en) 1990-04-26 1994-03-29 Calgene, Inc. Plant medium-chain-preferring acyl-ACP thioesterases and related methods
US5512482A (en) * 1990-04-26 1996-04-30 Calgene, Inc. Plant thioesterases
US5455167A (en) 1991-05-21 1995-10-03 Calgene Inc. Medium-chain thioesterases in plants
US5639790A (en) 1991-05-21 1997-06-17 Calgene, Inc. Plant medium-chain thioesterases
US5850022A (en) 1992-10-30 1998-12-15 Calgene, Inc. Production of myristate in plant cells
US5654495A (en) 1992-10-30 1997-08-05 Calgene, Inc. Production of myristate in plant cells
EP0716708A1 (de) * 1993-09-03 1996-06-19 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Mittelkettenspezifische thioesterasen
WO1995013390A2 (en) * 1993-11-10 1995-05-18 Calgene, Inc. Plant acyl acp thioesterase sequences
US5807893A (en) 1993-11-18 1998-09-15 Voelker; Toni Alois Plant thioesterases and use for modification of fatty acid composition in plant seed oils
CN101765661B (zh) 2007-06-01 2014-08-06 索拉兹米公司 在微生物中生产油
ES2545083T3 (es) * 2007-07-09 2015-09-08 Bayer Cropscience Nv Planta de Brassica que comprende alelos mutantes de acil graso-ACP tioesterasa
US7982035B2 (en) 2007-08-27 2011-07-19 Duquesne University Of The Holy Spirit Tricyclic compounds having antimitotic and/or antitumor activity and methods of use thereof
US20110099669A1 (en) * 2008-06-20 2011-04-28 Basf Plant Science Gmbh Plants having enhanced yield-related traits and a method for making the same
JP6109475B2 (ja) 2008-11-28 2017-04-05 テラヴィア ホールディングス， インコーポレイテッド 従属栄養微生物における、用途に応じた油の生産
CN102459569B (zh) * 2009-04-10 2018-02-23 Reg生命科学有限责任公司 脂肪酸衍生物的产生
CA3039432A1 (en) 2010-05-28 2011-12-01 Corbion Biotech, Inc. Tailored oils produced from recombinant heterotrophic microorganisms
US9249436B2 (en) * 2011-02-02 2016-02-02 Solazyme, Inc. Tailored oils produced from recombinant oleaginous microorganisms
US8951762B2 (en) * 2011-07-27 2015-02-10 Iowa State University Research Foundation, Inc. Materials and methods for using an acyl—acyl carrier protein thioesterase and mutants and chimeras thereof in fatty acid synthesis
CN102586350A (zh) * 2012-01-09 2012-07-18 北京化工大学 一种c8:0/c10:0/c12:0/c14:0中链脂肪酸及其乙酯的生产方法
KR20150001830A (ko) 2012-04-18 2015-01-06 솔라짐, 인코포레이티드 맞춤 오일

Also Published As

Publication number Publication date
EP2971024A1 (en) 2016-01-20
AU2018267601A1 (en) 2018-12-06
JP2016518112A (ja) 2016-06-23
MX2015011507A (es) 2016-04-07
EP2971024A4 (en) 2016-11-16
KR20150128770A (ko) 2015-11-18
AU2014236763B2 (en) 2018-08-23
BR112015023192A2 (pt) 2017-11-21
CA2904395A1 (en) 2014-09-25
WO2014151904A1 (en) 2014-09-25
CN105143458A (zh) 2015-12-09
AU2014236763A1 (en) 2015-10-01

Similar Documents

Publication Publication Date Title
BR112015023192A8 (pt) Tioesterases e células para a produção de óleos personalizados
CL2019002477A1 (es) Producción de ácidos graso poliinsaturados de cadena larga en células vegetales. (divisional solicitud 201403402)
CL2019002663A1 (es) Producción de ácidos grasos poliinsaturados de cadena larga en células vegetales. (divisional solicitud 201702448)
BR112017005370A2 (pt) acil-acp tioesterases e mutantes das mesmas
BR112016029394A2 (pt) métodos, reagentes e células para biossintetizar composto
MX2020004734A (es) Procesos para producir productos industriales de lipidos vegetales.
EA201600471A1 (ru) Экстрагированный растительный липид, содержащий длинноцепочечные полиненасыщенные жирные кислоты
BR112017001404A2 (pt) tioesterases variantes e métodos de utilização
EP3022310A4 (en) Microorganisms and methods for the production of fatty acids and fatty acid derived products
BR112015016021A2 (pt) processo para a produção de bio - nafta a partir de misturas complexas de gorduras & óleos de ocorrência natural
BR112015017920A8 (pt) Molécula de ácido nucleico codificando uma proteína transportadore de acila (acp)-acila tioesterase variante, cassete de expressão, vetor, tioesterase (te) variante de proteína transportadora de acila-acila (acp), célula hospedeira, produto de óleo, método para produzir uma planta, alga ou microalga, que produz um óleo com um perfil de ácido graxo desejado, e método para produzir um óleo
BR112017013394A2 (pt) fabricação de proteína
EA201491956A1 (ru) Масла с высоким содержанием олеиновой кислоты
MX2017006304A (es) Materiales y metodos para aumentar el contenido de tocoferol en semillas oleaginosas.
BR112014018682A8 (pt) Processo para produção de ácidos graxos, sn-regio mono-acilglicerol (mag), sn- regio diacil-gliceróis (dag) e glicerol; e processo para produção de ácidos graxos e glicerol
BR102013022698A8 (pt) Éster de poliglicerol, processo para a produção do mesmo, seu uso e formulação cosmética ou farmacêutica
BR112016029391A2 (pt) métodos, reagentes e células para biossintetizar compostos
BR112014009333A2 (pt) processo para produzir um éster de ácido graxo epoxidizado a partir de uma gordura ou óleo natural epoxidizado
CL2012003644A1 (es) Polinucleotido que comprende una aciltransferasas; celula huesped que comprende dicho polinucleotido; metodo para la fabricacion de un polipeptido ; metodo para la fabricacion de acidos grasos poliinsaturados.
MY185442A (en) Production of fatty acid alkyl esters with caustic treatment
FI20125509A (fi) Modifioitu mäntyöljyrasvahappo
UA117935C2 (uk) Спосіб одержання l-амінокислот
BR112017020906A2 (pt) microalgas adaptadas para condições de cultura heterotróficas
BR112018012193A2 (pt) variantes do polipeptídeo de fusão relacionados à ômega-hidroxilase com propriedades melhoradas
BRPI1011100A2 (pt) Emulsão ou gel para a produção de alimentos contendo ácido graxo ômega-3.

Legal Events

Date Code Title Description
B25D Requested change of name of applicant approved

Owner name: TERRAVIA HOLDINGS, INC. (US)

B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements