WO 2014/151904 PCT/US2014/026644 THIOESTERASES AND CELLS FOR PRODUCTION OF TAILORED OILS Cross Reference to related Applications [0001] This application is a Continuation-in-part of United States Patent 5 Application No. 13/837,996, filed March 15, 2013, and claims the benefit of United States Provisional Patent Application Serial No. 61/791,861, filed March 15, 2013, and United States Provisional Patent Application Serial No. 61/917,217, filed December 17, 2013, each of which is hereby incorporated by reference herein in its entirety. 10 Background [0002] Certain organisms including plants and some microalgae use a type II fatty acid biosynthetic pathway, characterized by the use of discrete, monofunctional enzymes for fatty acid synthesis. In contrast, mammals and fungi use a single, large, multifunctional protein. 15 [0003] Type II fatty acid biosynthesis typically involves extension of a growing acyl-ACP (acyl-carrier protein) chain by two carbon units followed by cleavage by an acyl-ACP thioesterase. In plants, two main classes of acyl-ACP thioesterases have been identified: (i) those encoded by genes of the FatA class, which tend to hydrolyze oleoyl-ACP into oleate (an 18:1 fatty acid) and ACP, and (ii) 20 those encoded by genes of the FatB class, which liberate C8-C16 fatty acids from corresponding acyl-ACP molecules. [0004] Different FatB genes from various plants have specificities for different acyl chain lengths. As a result, different gene products will produce different fatty acid profiles in plant seeds. See, US Patent Nos. 5,850,022; 5,723,761; 25 5,639,790; 5,807,893; 5,455,167; 5,654,495; 5,512,482;5,298,421;5,667,997; and 5,344,771; 5,304,481. Recently, FatB genes have been cloned into oleaginous microalgae to produce triglycerides with altered fatty acid profiles. See, W02010/063032, W02011/150411, W02012/106560, and W02013/158938. 1 WO 2014/151904 PCT/US2014/026644 Summary [0005] In various aspects, the invention(s) contemplated herein may include, but need not be limited to, any one or more of the following embodiments: [0006] Embodiment 1: A nucleic acid construct including a regulatory 5 element and a FatB gene expressing an active acyl-ACP thioesterase operable to produce an altered fatty acid profile in an oil produced by a cell expressing the nucleic acid construct, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 5 of Table la, the sequence having at least 94.6% sequence identity with each of SEQ ID NOs: 88, 82, 85, and 103, and optionally 10 wherein the fatty acid of the oil is enriched in C8 and C10 fatty acids. [00071 Embodiment 2: A nucleic acid construct including a regulatory element and a FatB gene expressing an active acyl-ACP thioesterase operable to produce an altered fatty acid profile in an oil produced by a cell expressing the nucleic acid construct, wherein the FatB gene expresses a protein having an amino acid 15 sequence falling within one of clades 1-12 of Table la. [0008] Embodiment 3: The nucleic acid construct of embodiment 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 1 of Table la, the sequence having at least 85.9% sequence identity with each of SEQ ID NOs: 19, 161, 22, and 160, and optionally wherein the fatty acid of the oil is 20 enriched in C14 and C16 fatty acids. [0009] Embodiment 4: The nucleic acid construct of embodiment 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 2 of Table la, the sequence having at least 89.5% sequence identity with each of SEQ ID NOs: 134-136, 132, 133, 137, 124, 122, 123, 125, and optionally wherein the fatty 25 acid of the oil is enriched in C12 and C14 fatty acids. [0010] Embodiment 5: The nucleic acid construct of embodiment 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 3 of Table la, the sequence having at least 92.5% sequence identity with each of SEQ ID NOs: 126 and 127, and optionally wherein the fatty acid of the oil is enriched in 30 C12 and C14 fatty acids. [0011] Embodiment 6: The nucleic acid construct of embodiment 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 2 WO 2014/151904 PCT/US2014/026644 4 of Table la, the sequence having at least 83.8% sequence identity with SEQ ID NO: 79, and optionally wherein the fatty acid of the oil is enriched in C12 and C14 fatty acids. [0012] Embodiment 7: The nucleic acid construct of embodiment 2, wherein 5 the FatB gene expresses a protein having an amino acid sequence falling within clade 6 of Table la, the sequence having at least 99.9% sequence identity with each of SEQ ID NOs: 111 and 110, and optionally wherein the fatty acid of the oil is enriched in C10 fatty acids. [0013] Embodiment 8: The nucleic acid construct of embodiment 2, wherein 10 the FatB gene expresses a protein having an amino acid sequence falling within clade 7 of Table la, the sequence having at least 89.5% sequence identity with each of SEQ ID NOs: 73, 106, 185, 172, 171, 173, 174, and optionally wherein the fatty acid of the oil is enriched in C10 and C12 fatty acids. [0014] Embodiment 9: The nucleic acid construct of embodiment 2, wherein 15 the FatB gene expresses a protein having an amino acid sequence falling within clade 8 of Table la, the sequence having at least 85.9% sequence identity with each of SEQ ID NOs: 112, 113, 142, 145, 143, 144, 139, 140, 138, 141, and optionally wherein the fatty acid of the oil is enriched in C12 and C14 fatty acids. [00151 Embodiment 10: The nucleic acid construct of embodiment 2, 20 wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 9 of Table la, the sequence having at least 83.8% sequence identity with each of SEQ ID NOs: 187-189, and optionally wherein the fatty acid of the oil is enriched in C12 and C14 fatty acids. [0016] Embodiment 11: The nucleic acid construct of embodiment 2, 25 wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 10 of Table 1 a, the sequence having at least 95.9% sequence identity with each of SEQ ID NOs: 147, 149, 146, 150, 152, 151, 148, 154, 156, 155, 157, 108, 75, 190, 191, and 192, and optionally wherein the fatty acid of the oil is enriched in C14 and C16 fatty acids. 30 [00171 Embodiment 12: The nucleic acid construct of embodiment 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 11 of Table la, the sequence having at least 88.7% sequence identity 3 WO 2014/151904 PCT/US2014/026644 with SEQ ID NO: 121, and optionally wherein the fatty acid of the oil is enriched in C14 and C16 fatty acids. [00181 Embodiment 13: The nucleic acid construct of embodiment 2, wherein the FatB gene expresses a protein having an amino acid sequence falling 5 within clade 12 of Table la, the sequence having at least 72.8% sequence identity with each of SEQ ID NOs: 129 and 186, and optionally wherein the fatty acid of the oil is enriched in C16 fatty acids. [0019] Embodiment 14: An isolated nucleic acid or recombinant DNA construct including a nucleic acid, wherein the nucleic acid has at least 80% sequence 10 identity to any of SEQ ID NOS: 2, 3, 5, 6, 8, 9, 11, 12, 14, 15, 17, 18, 20, 21, 23, 24, 26, 27, 29, 30, 32, 33, 35, 36, 38, 39, 41, 42, 44, 45, 47, 48, 50, 51, 53, 54, 56, 57, 59, 60, 62, 63, 65, 66, 68, 69, 71, 72, 74, 76, 78, 80, 81, 83, 84, 86, 87, 89, 90, 92, 93, 95, 96, 98, 99, 101, 102, 104, 105, 107, 109 or any equivalent sequences by virtue of the degeneracy of the genetic code. 15 [0020] Embodiment 15: An isolated nucleic acid sequence encoding a protein or a host cell expressing a protein having at least 80% sequence identity to any of SEQ ID NOS: 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 75, 77, 79, 82, 85, 88, 91, 94, 97, 100, 103, 106, 108, 110-192 or a fragment thereof having acyl-ACP thioesterase activity. 20 [0021] Embodiment 16: The isolated nucleic acid of embodiment 15, wherein, the protein has acyl-ACP thioesterase activity operable to alter the fatty acid profile of an oil produced by a recombinant cell including that sequence. [0022] Embodiment 17: A method of producing a recombinant cell that produces an altered fatty acid profile, the method including transforming the cell with 25 a nucleic acid according to any of embodiments 1-3. [0023] Embodiment 18: A host cell produced by the method of embodiment 17. [0024] Embodiment 19: The host cell of embodiment 18, wherein the host cell is selected from a plant cell, a microbial cell, and a microalgal cell. 30 [00251 Embodiment 20: A method for producing an oil or oil-derived product, the method including cultivating a host cell of embodiment 5 or 6, and 4 WO 2014/151904 PCT/US2014/026644 extracting oil produced thereby, optionally wherein the cultivation is heterotrophic growth on sugar. [0026] Embodiment 21: The method of embodiment 20, further including producing a fatty acid, fuel, chemical, or other oil-derived product from the oil. 5 [00271 Embodiment 22: An oil produced by the method of embodiment 20, optionally having a fatty acid profile including at least 20% C8, C10, C12, C14 or C16 fatty acids. [0028] Embodiment 23: An oil-derived product produced by the method of embodiment 21. 10 [0029] Embodiment 24: The oil of embodiment 23, wherein the oil is produced by a microalgae and optionally, lacks C24-alpha sterols. Description of Illustrative Embodiments of the Invention Definitions [0030] As used with respect to nucleic acids, the term "isolated" refers to a 15 nucleic acid that is free of at least one other component that is typically present with the naturally occurring nucleic acid. Thus, a naturally occurring nucleic acid is isolated if it has been purified away from at least one other component that occurs naturally with the nucleic acid. [0031] A "natural oil" or "natural fat" shall mean a predominantly triglyceride 20 oil obtained from an organism, where the oil has not undergone blending with another natural or synthetic oil, or fractionation so as to substantially alter the fatty acid profile of the triglyceride. In connection with an oil comprising triglycerides of a particular regiospecificity, the natural oil or natural fat has not been subjected to interesterification or other synthetic process to obtain that regiospecific triglyceride 25 profile, rather the regiospecificity is produced naturally, by a cell or population of cells. In connection with a natural oil or natural fat, and as used generally throughout the present disclosure, the terms oil and fat are used interchangeably, except where otherwise noted. Thus, an "oil" or a "fat" can be liquid, solid, or partially solid at room temperature, depending on the makeup of the substance and other conditions. 30 Here, the term "fractionation" means removing material from the oil in a way that 5 WO 2014/151904 PCT/US2014/026644 changes its fatty acid profile relative to the profile produced by the organism, however accomplished. The terms "natural oil" and "natural fat" encompass such oils obtained from an organism, where the oil has undergone minimal processing, including refining, bleaching and/or degumming, which does not substantially change its 5 triglyceride profile. A natural oil can also be a "noninteresterified natural oil", which means that the natural oil has not undergone a process in which fatty acids have been redistributed in their acyl linkages to glycerol and remain essentially in the same configuration as when recovered from the organism. [0032] "Exogenous gene" shall mean a nucleic acid that codes for the 10 expression of an RNA and/or protein that has been introduced into a cell (e.g. by transformation/transfection), and is also referred to as a "transgene". A cell comprising an exogenous gene may be referred to as a recombinant cell, into which additional exogenous gene(s) may be introduced. The exogenous gene may be from a different species (and so heterologous), or from the same species (and so 15 homologous), relative to the cell being transformed. Thus, an exogenous gene can include a homologous gene that occupies a different location in the genome of the cell or is under different control, relative to the endogenous copy of the gene. An exogenous gene may be present in more than one copy in the cell. An exogenous gene may be maintained in a cell, for example, as an insertion into the genome 20 (nuclear or plastid) or as an episomal molecule. [0033] "Fatty acids" shall mean free fatty acids, fatty acid salts, or fatty acyl moieties in a glycerolipid. It will be understood that fatty acyl groups of glycerolipids can be described in terms of the carboxylic acid or anion of a carboxylic acid that is produced when the triglyceride is hydrolyzed or saponified. 25 [0034] "Microalgae" are microbial organisms that contain a chloroplast or other plastid, and optionally that are capable of performing photosynthesis, or a prokaryotic microbial organism capable of performing photosynthesis. Microalgae include obligate photoautotrophs, which cannot metabolize a fixed carbon source as energy, as well as heterotrophs, which can live solely off of a fixed carbon source. 30 Microalgae include unicellular organisms that separate from sister cells shortly after cell division, such as Chlamydomonas, as well as microbes such as, for example, Volvox, which is a simple multicellular photosynthetic microbe of two distinct cell types. Microalgae include cells such as Chlorella, Dunaliella, and Prototheca. 6 WO 2014/151904 PCT/US2014/026644 Microalgae also include other microbial photosynthetic organisms that exhibit cell cell adhesion, such as Agmenellum, Anabaena, and Pyrobotrys. Microalgae also include obligate heterotrophic microorganisms that have lost the ability to perform photosynthesis, such as certain dinoflagellate algae species and species of the genus 5 Prototheca. [00351 An "oleaginous" cell is a cell capable of producing at least 20% lipid by dry cell weight, naturally or through recombinant or classical strain improvement. An "oleaginous microbe" or "oleaginous microorganism" is a microbe, including a microalga that is oleaginous. 10 [0036] The term "percent sequence identity," in the context of two or more amino acid or nucleic acid sequences, refers to two or more sequences or subsequences that are the same or have a specified percentage of amino acid residues or nucleotides that are the same, when compared and aligned for maximum correspondence, as measured using a sequence comparison algorithm or by visual 15 inspection. For sequence comparison to determine percent nucleotide or amino acid identity, typically one sequence acts as a reference sequence, to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are input into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. The sequence 20 comparison algorithm then calculates the percent sequence identity for the test sequence(s) relative to the reference sequence, based on the designated program parameters. Optimal alignment of sequences for comparison can be conducted using the NCBI BLAST software (ncbi.nlm.nih.gov/BLAST/) set to default parameters. For example, to compare two nucleic acid sequences, one may use blastn with the 25 "BLAST 2 Sequences" tool Version 2.0.12 (Apr. 21, 2000) set at the following default parameters: Matrix: BLOSUM62; Reward for match: 1; Penalty for mismatch: -2; Open Gap: 5 and Extension Gap: 2 penalties; Gap x drop-off: 50; Expect: 10; Word Size: 11; Filter: on. For a pairwise comparison of two amino acid sequences, one may use the "BLAST 2 Sequences" tool Version 2.0.12 (Apr. 21, 30 2000) with blastp set, for example, at the following default parameters: Matrix: BLOSUM62; Open Gap: 11 and Extension Gap: 1 penalties; Gap x drop-off 50; Expect: 10; Word Size: 3; Filter: on. 7 WO 2014/151904 PCT/US2014/026644 [00371 In connection with a natural oil, a "profile" is the distribution of particular species or triglycerides or fatty acyl groups within the oil. A "fatty acid profile" is the distribution of fatty acyl groups in the triglycerides of the oil without reference to attachment to a glycerol backbone. Fatty acid profiles are typically 5 determined by conversion to a fatty acid methyl ester (FAME), followed by gas chromatography (GC) analysis with flame ionization detection (FID). The fatty acid profile can be expressed as one or more percent of a fatty acid in the total fatty acid signal determined from the area under the curve for that fatty acid. FAME-GC-FID measurement approximate weight percentages of the fatty acids. 10 [0038] As used herein, an oil is said to be "enriched" in one or more particular fatty acids if there is at least a 10% increase in the mass of that fatty acid in the oil relative to the non-enriched oil. For example, in the case of a cell expressing a heterologous FatB gene described herein, the oil produced by the cell is said to be enriched in, e.g., C8 and C16 fatty acids if the mass of these fatty acids in the oil is at 15 least 10% greater than in oil produced by a cell of the same type that does not express the heterologous FatB gene (e.g., wild type oil). [00391 "Recombinant" is a cell, nucleic acid, protein or vector that has been modified due to the introduction of an exogenous nucleic acid or the alteration of a native nucleic acid. Thus, e.g., recombinant (host) cells can express genes that are not 20 found within the native (non-recombinant) form of the cell or express native genes differently than those genes are expressed by a non-recombinant cell. Recombinant cells can, without limitation, include recombinant nucleic acids that encode a gene product or suppression elements such as mutations, knockouts, antisense, interfering RNA (RNAi) or dsRNA that reduce the levels of active gene product in a cell. A 25 "recombinant nucleic acid" is a nucleic acid originally formed in vitro, in general, by the manipulation of nucleic acid, e.g., using polymerases, ligases, exonucleases, and endonucleases, using chemical synthesis, or otherwise is in a form not normally found in nature. Recombinant nucleic acids may be produced, for example, to place two or more nucleic acids in operable linkage. Thus, an isolated nucleic acid or an 30 expression vector formed in vitro by nucleic by ligating DNA molecules that are not normally joined in nature, are both considered recombinant for the purposes of this invention. Recombinant nucleic acids can also be produced in other ways; e.g., using chemical DNA synthesis. Once a recombinant nucleic acid is made and introduced 8 WO 2014/151904 PCT/US2014/026644 into a host cell or organism, it may replicate using the in vivo cellular machinery of the host cell; however, such nucleic acids, once produced recombinantly, although subsequently replicated intracellularly, are still considered recombinant for purposes of this invention. Similarly, a "recombinant protein" is a protein made using 5 recombinant techniques, i.e., through the expression of a recombinant nucleic acid. [0040] Embodiments of the present invention relate to the use of FatB genes isolated from plants, which can be expressed in a host cell in order to alter the fatty acid profile of an oil produced by the recombinant cell. Although the microalga, Prototheca moriformis, was used to screen the genes for ability to the alter fatty acid 10 profile, the genes are useful in a wide variety of host cells. For example, the genes can be expressed in bacteria, other microalgae, or higher plants. The genes can be expressed in higher plants according to the methods of US Patent Nos. 5,850,022; 5,723,761; 5,639,790; 5,807,893; 5,455,167; 5,654,495; 5,512,482;5,298,421;5,667,997; 5,344,771; and 5,304,481. The fatty acids can be 15 further converted to triglycerides, fatty aldehydes, fatty alcohols and other oleochemicals either synthetically or biosynthetically. [0041] In specific embodiments, triglycerides are produced by a host cell expressing a novel FatB gene. A triglyceride-containing natural oil can be recovered from the host cell. The natural oil can be refined, degummed, bleached and/or 20 deodorized. The oil, in its natural or processed form, can be used for foods, chemicals, fuels, cosmetics, plastics, and other uses. In other embodiments, the FatB gene may not be novel, but the expression of the gene in a microalga is novel. [0042] The genes can be used in a variety of genetic constructs including plasmids or other vectors for expression or recombination in a host cell. The genes 25 can be codon optimized for expression in a target host cell. The proteins produced by the genes can be used in vivo or in purified form. [0043] For example, the gene can be prepared in an expression vector comprising an operably linked promoter and 5'UTR. Where a plastidic cell is used as the host, a suitably active plastid targeting peptide can be fused to the FATB gene, 30 as in the examples below. Generally, for the newly identified FATB genes, there are roughly 50 amino acids at the N-terminal that constitute a plastid transit peptide, which are responsible for transporting the enzyme to the chloroplast. In the examples below, this transit peptide is replaced with a 38 amino acid sequence that is effective 9 WO 2014/151904 PCT/US2014/026644 in the Prototheca moriformis host cell for transporting the enzyme to the plastids of those cells. Thus, the invention contemplates deletions and fusion proteins in order to optimize enzyme activity in a given host cell. For example, a transit peptide from the host or related species may be used instead of that of the newly discovered plant 5 genes described here. [0044] A selectable marker gene may be included in the vector to assist in isolating a transformed cell. Examples of selectable markers useful in microlagae include sucrose invertase and antibiotic resistance genes. [00451 The gene sequences disclosed can also be used to prepare antisense, or 10 inhibitory RNA (e.g., RNAi or hairpin RNA) to inhibit complementary genes in a plant or other organism. [0046] FatB genes found to be useful in producing desired fatty acid profiles in a cell are summarized below in Table 1. Nucleic acids or proteins having the sequence of SEQ ID NOS: 1-109 can be used to alter the fatty acid profile of a 15 recombinant cell. Variant nucleic acids can also be used; e.g., variants having at least 70, 80, 85, 90, 95, 96, 97, 98, or 99% sequence identity to SEQ ID NOS: 2, 3, 5, 6, 8, 9,11,12,14,15,17,18,20,21,23,24,26,27,29,30,32,33,35,36,38,39,41,42, 44, 45, 47, 48, 50, 51, 53, 54, 56, 57, 59, 60, 62, 63, 65, 66, 68, 69, 71, 72, 74, 76, 78, 80, 81, 83, 84, 86, 87, 89, 90, 92, 93, 95, 96, 98, 99, 101, 102, 104, 105, 107 or 109. 20 Codon optimization of the genes for a variety of host organisms is contemplated, as is the use of gene fragments. Preferred codons for Prototheca strains and for Chlorella protothecoides are shown below in Tables 2 and 3, respectively. Codon usage for Cuphea wrightii is shown in Table 3a. Codon usage for Arabidopsis is shown in Table 3b; for example, the most preferred of codon for each amino acid can be 25 selected. Codon tables for other organisms including microalgae and higher plants are known in the art. In some embodiments, the first and/or second most preferred Prototheca codons are employed for codon optimization. In specific embodiments, the novel amino acid sequences contained in the sequence listings below are converted into nucleic acid sequences according to the most preferred codon usage in 30 Prototheca, Chlorella, Cuphea wrightii, or Arabidopsis as set forth in tables 2 through 3b or nucleic acid sequences having at least 70, 80, 85, 90, 95, 96, 97, 98, or 99% sequence identity to these derived nucleic acid sequences. 10 WO 2014/151904 PCT/US2014/026644 [00471 In embodiments of the invention, there is protein or a nucleic acid encoding a protein having any of SEQ ID NOS: 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34,37,40,43,46,49,52,55,58,61,64,67,70,73,75,77,79,82,85,88,91,94,97, 100, 103, 106, 108, or 110-192. In an embodiment, there is protein or a nucleic acid 5 encoding a protein having at least 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or 100% sequence identity with any of SEQ ID NOS: 1, 4, 7, 10, 13, 16, 19, 22, 25, 28,31,34,37,40,43,46,49,52,55,58,61,64,67,70,73,75,77,79,82,85,88,91, 94, 97, 100, 103, 106, 108, or 110-192. In certain embodiments, the invention encompasses a fragment any of the above-described proteins or nucleic acids 10 (including fragments of protein or nucleic acid variants), wherein the protein fragment has acyl-ACP thioesterase activity or the nucleic acid fragment encodes such a protein fragment. In other embodiments, the fragment includes a domain of an acyl-ACP thioesterase that mediates a particular function, e.g., a specificity-determining domain. Illustrative fragments can be produced by C-terminal and/or N-terminal truncations 15 and include at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% of the full-length sequences disclosed herein. [0048] In certain embodiments, percent sequence identity for variants of the nucleic acids or proteins discussed above can be calculated by using the full-length nucleic acid sequence (e.g., one of SEQ ID NOS: 2, 3, 5, 6, 8, 9, 11, 12, 14, 15, 17, 20 18,20,21,23,24,26,27,29,30,32,33,35,36,38,39,41,42,44,45,47,48,50,51, 53,54,56,57,59,60,62,63,65,66,68,69,71,72,74,76,78,80,81,83,84,86,87, 89, 90, 92, 93, 95, 96, 98, 99, 101, 102, 104, 105, 107 or 109) or full-length amino acid sequence (e.g., one of SEQ ID NOS: 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40,43,46,49,52,55,58,61,64,67,70,73,75,77,79,82,85,88,91,94,97, 100, 25 103, 106, 108, or 110-192) as the reference sequence and comparing the full-length test sequence to this reference sequence. In some embodiments relating to fragments, percent sequence identity for variants of nucleic acid or protein fragments can be calculated over the entire length of the fragment. [0049] The nucleic acids can be in isolated form, or part of a vector or other 30 construct, chromosome or host cell. It has been found that is many cases the full length gene (and protein) is not needed; for example, deletion of some or all of the N terminal hydrophobic domain (typically an 18 amino acid domain starting with LPDW) yields a still-functional gene. In addition, fusions of the specificity 11 WO 2014/151904 PCT/US2014/026644 determining regions of the genes in Table 1 with catalytic domains of other acyl-ACP thioesterases can yield functional genes. Thus, in certain embodiments, the invention encompasses functional fragments (e.g., specificity determining regions) of the disclosed nucleic acid or amino acids fused to heterologous acyl-ACP thioesterase 5 nucleic acid or amino acid sequences, respectively. Table 1: FatB genes according to embodiments of the present invention Species Gene Name Sequence Variant Amino Native Protothec (relative to Acid CDS a dominant Sequenc nucleotid moriformi transcript e of CDS e s codon identified) (no sequence optimized additiona (not nucleotid tags) codon- e optimize sequence d, no of CDS additiona 1 cloning sites) Cinnamomu CcFATB1b M25L, M322R, SEQ ID SEQ ID SEQ ID m camphora AT367-D368 NO: 1 NO: 2 NO: 3 Cinnamomu CcFATB4 "wild-type" SEQ ID SEQ ID SEQ ID m camphora NO: 4 NO: 5 NO: 6 Cinnamomu CcFATB3 "wild-type" SEQ ID SEQ ID SEQ ID m camphora NO: 7 NO: 8 NO: 9 Cuphea ChsFATB 1 "wild-type" SEQ ID SEQ ID SEQ ID hyssopifolia NO: 10 NO:11 NO: 12 Cuphea ChsFATB2 "wild-type" SEQ ID SEQ ID SEQ ID hyssopifolia NO: 13 NO: 14 NO: 15 12 WO 2014/151904 PCT/US2014/026644 Cuphea ChsFATB2b +a.a.248-259 SEQ ID SEQ ID SEQ ID hyssopifolia NO: 16 NO: 17 NO: 18 Cuphea ChsFATB3 "wild-type" SEQ ID SEQ ID SEQ ID hyssopifolia NO: 19 NO: 20 NO: 21 Cuphea ChsFATB3b V2041,C239F, SEQ ID SEQ ID SEQ ID hyssopifolia E243D, M251V NO: 22 NO: 23 NO: 24 Cuphea CuPSR23FATB "wild-type" SEQ ID SEQ ID SEQ ID PSR23 3 NO: 25 NO: 26 NO: 27 Cuphea CwFATB3 "wild-type" SEQ ID SEQ ID SEQ ID wrightii NO: 28 NO: 29 NO: 30 Cuphea CwFATB4a "wild-type" SEQ ID SEQ ID SEQ ID wrightii NO: 31 NO: 32 NO: 33 Cuphea CwFATB4b "wild-type" SEQ ID SEQ ID SEQ ID wrightii NO: 34 NO: 35 NO: 36 Cuphea CwFATB5 "wild-type" SEQ ID SEQ ID SEQ ID wrightii NO: 37 NO: 38 NO: 39 Cuphea ChtFATB 1 a "wild-type" SEQ ID SEQ ID SEQ ID heterophylla NO: 40 NO: 41 NO: 42 Cuphea ChtFATB1b P16S, T20P, SEQ ID SEQ ID SEQ ID heterophylla G94S, G105W, NO: 43 NO: 44 NO: 45 S293F, L305F Cuphea ChtFATB2b "wild-type" SEQ ID SEQ ID SEQ ID heterophylla NO: 46 NO: 47 NO: 48 Cuphea ChtFATB2a S17P, P21S, SEQ SEQ ID SEQ ID heterophylla T28N,L30P, IDO NO: 50 NO: 51 S33L, G76D, NO: 49 S78P, G137W Cuphea ChtFATB2c G76D, S78P SEQ ID SEQ ID SEQ ID heterophylla NO: 52 NO: 53 NO: 54 Cuphea ChtFATB2d S21P, T28N, SEQ ID SEQ ID SEQ ID heterophylla L30P, S33L, NO: 55 NO: 56 NO: 57 G76D, R97L, H124L, W127L, 13 WO 2014/151904 PCT/US2014/026644 1132S, K258N, C303R, E309G, K334T, T386A Cuphea ChtFATB2e G76D, R97L, SEQ ID SEQ ID SEQ ID heterophylla H124L, 1132S, NO: 58 NO: 59 NO: 60 G152S, H165L, T21 IN, K258N, C303R, E309G, K334T, T386A Cuphea ChtFATB2f R97L, H124L, SEQ ID SEQ ID SEQ ID heterophylla 1132S, G152S, NO: 61 NO: 62 NO: 63 H165L, T211N Cuphea ChtFATB2g A6T, A16V, S17P, SEQ ID SEQ ID SEQ ID heterophylla G76D, R97L, NO: 64 NO: 65 NO: 66 H124L, I132S, S143I, G152S, A157T, H165L, T211N, G414A Cuphea ChtFATB3a "wild-type" SEQ ID SEQ ID SEQ ID heterophylla NO: 67 NO: 68 NO: 69 Cuphea ChtFATB3b C67G, H72Q, SEQ ID SEQ ID SEQ ID heterophylla L128F, N1791 NO: 70 NO: 71 NO: 72 Cuphea CvisFATB 1 published SEQ ID N/A SEQ ID viscosissima NO: 73 NO: 74 Cuphea CvisFATB2 published SEQ ID N/A SEQ ID viscosissima NO: 75 NO: 76 Cuphea CvisFATB3 published SEQ ID N/A SEQ ID viscosissima NO: 77 NO: 78 Cuphea CcalcFATBI "wild-type" SEQ ID SEQ ID SEQ ID calcarata NO: 79 80 81 Cuphea CpaiFATB1 "wild-type" SEQ ID SEQ ID SEQ ID painteri NO: 82 83 84 14 WO 2014/151904 PCT/US2014/026644 Cuphea ChookFATB4 "wild-type" SEQ ID SEQ ID SEQ ID hookeriana NO: 85 86 87 Cuphea CaFATBI "wild-type" SEQ ID SEQ ID SEQ ID avigera var. NO: 88 89 90 pulcherrima Cuphea CPauFATB 1 "wild-type" SEQ ID SEQ ID SEQ ID paucipetala NO: 91 92 93 Cuphea CprocFATB1 "wild-type" SEQ ID SEQ ID SEQ ID procumbens NO: 94 95 96 Cuphea CprocFATB2 "wild-type" SEQ ID SEQ ID SEQ ID procumbens NO: 97 98 99 Cuphea CprocFATB3 "wild-type" SEQ ID SEQ ID SEQ ID procumbens NO: 100 101 102 Cuphea CigneaFATBI "wildtype"; partial SEQ ID SEQ ID SEQ ID ignea (missing N- NO: 103 104 105 terminal portion of native transit peptide, fused to CpSAD1tp trimm ed transit peptide) Consensus JcFATB 1 Consensus SEQ ID None, SEQ ID sequence NO: 106 can be NO: 107 codon optimize d for a given 15 WO 2014/151904 PCT/US2014/026644 host Consensus JcFATB2 Consensus SEQ ID None, SEQ ID sequence NO: 108 can be NO: 109 codon optimize d for a given host In certain embodiments, a host cell (e.g. plant or microalgal cell) is transformed to produce a recombinant FATB protein falling into one of clades 1-12 of Table la. These clades were determined by sequence alignment and observation of changes in 5 fatty acid profile when expressed in Prototheca. See Example 5. The FATB amino acid sequence can fall within x% amino acid sequence identity of each sequence in that clade listed in Table la, where x is a first second or third cutoff value, also listed in Table la. 16 WO 2014/151904 PCT/US2014/026644 Table la: Groupings of Novel FatB genes into clades. Clade Amino Acid SEQ ID Nos. in Clade Example Function First Cutoff Second Third No. (see Table 6) Value Cutoff Cutoff (minimum Value Value % amino acid identity to members of clade) 1 ChsFATB3 (SEQ ID NO: 19) Increase C14/C16 85.9 97.4 98 ChsFATB3d (SEQ ID NO: 161) fatty acids ChsFATB3b (SEQ ID NO: 22) ChsFATB3c (SEQ ID NO: 160) 2 ChtFATB 1a.2 (SEQ ID NO: 134) Increase C12/C14 89.5 95 98 ChtFATB 1a.3 (SEQ ID NO: 135) fatty acids ChtFATB 1a.4 (SEQ ID NO: 136) ChtFATB 1a (SEQ ID NO: 132)_ ChtFATB 1a.1 (SEQ ID NO: 133) ChtFATB1b (SEQ ID NO: 137) CwFATB5b (SEQ ID NO: 124) CwFATB5 (SEQ ID NO: 122) CwFATB5a (SEQ ID NO: 123) CwFATB5c (SEQ ID NO: 125) 3 CwFATB5.1 (SEQ ID NO: 126) Increase C12/C14 92.5 95 98 CwFATB5.l a (SEQ ID NO: 127) fatty acids 4 CcalcFATB1 (SEQ ID NO: 79) Increase C12/C14 83.8 93 95 fatty acids 5 CaFATB1 (SEQ ID NO: 88) Increase C8/C1O 94.6 96 98 CpaiFATB1 (SEQ ID NO: 82) fatty acids ChookFATB4 (SEQ ID NO: 85) CigneaFATB1 (SEQ ID NO: 103) 6 CuPSR23FATB3b (SEQ ID NO: 111) Increase C10 fatty 99.9 CuPSR23FATB3 (SEQ ID NO: 110) acids 7 CvisFATB1 (SEQ ID NO: 73) Increase C1O/C12 89.5 93 96 JcFATB1/SzFATB1 (SEQ ID NO: 106) fatty acids 17 WO 2014/151904 PCT/US2014/026644 CgFATB1b (SEQ ID NO: 185) CprocFATB1 (SEQ ID NO: 172) CpauFATB1 (SEQ ID NO: 171) CprocFATB2 (SEQ ID NO: 173) CprocFATB3 (SEQ ID NO: 174) 8 CwFATB3 (SEQ ID NO: 112) Increase C12/C14 85.9 98.9 99.5 CwFATB3a (SEQ ID NO: 113) fatty acids ChtFATB2e (SEQ ID NO: 142) ChtFATB2h (SEQ ID NO: 145) ChtFATB2f (SEQ ID NO: 143) ChtFATB2g (SEQ ID NO: 144) ChtFATB2a (SEQ ID NO: 139) ChtFATB2c (SEQ ID NO: 140) ChtFATB2b (SEQ ID NO: 138) ChtFATB2d (SEQ ID NO: 141) 9 CcrFATB2c (SEQ ID NO: 187) Increase C12/C14 83.8 90 95 CcrFATB2 (SEQ ID NO: 188) fatty acids CcrFATB2b (SEQ ID NO: 189) 10 ChtFATB3b (SEQ ID NO: 147) Increase C14/C16 95.9 98 99 ChtFATB3d (SEQ ID NO: 149) fatty acids ChtFATB3a (SEQ ID NO: 146) ChtFATB3e (SEQ ID NO: 150) ChtFATB3g (SEQ ID NO: 152) ChtFATB3f (SEQ ID NO: 151) ChtFATB3c (SEQ ID NO: 148) ChsFATB2 (SEQ ID NO: 154) ChsFATB2c (SEQ ID NO: 156) ChsFATB2b (SEQ ID NO: 155) ChsFATB2d (SEQ ID NO: 157) JcFATB2/SzFATB2 (SEQ ID NO: 108) CvisFATB2 (SEQ ID NO: 75) CcrFATB1 (SEQ ID NO: 190) CcrFATB1b (SEQ ID NO: 191) CcrFATB1c (SEQ ID NO: 192) 11 Increase C14/C16 88.7 94.5 97 CwFATB4b.1 (SEQ ID NO: 121) fatty acids 12 CcFATB3 (SEQ ID NO: 129) Increase C16 fatty 72.8 85 90 UcFATB3 (SEQ ID NO: 186) acids (predicted) 18 WO 2014/151904 PCT/US2014/026644 Table 2: Preferred codon usage in Prototheca strains Ala GCG 345 (0.36) Asn AAT 8 (0.04) GCA 66 (0.07) AAC 201 (0.96) GCT 101 (0.11) 5 GCC 442 (0.46) Pro CCG 161 (0.29) CCA 49 (0.09) Cys TGT 12(0.10) CCT 71(0.13) TGC 105 (0.90) CCC 267 (0.49) 10 Asp GAT 43 (0.12) Gln CAG 226 (0.82) GAC 316(0.88) CAA 48(0.18) Glu GAG 377 (0.96) Arg AGG 33 (0.06) GAA 14 (0.04) AGA 14 (0.02) 15 CGG 102(0.18) Phe TTT 89 (0.29) CGA 49 (0.08) TTC 216(0.71) CGT 51(0.09) CGC 331 (0.57) Gly GGG 92 (0.12) 20 GGA 56 (0.07) Ser AGT 16 (0.03) GGT 76 (0.10) AGC 123 (0.22) GGC 559 (0.71) TCG 152 (0.28) TCA 31(0.06) 19 WO 2014/151904 PCT/US2014/026644 His CAT 42 (0.21) TCT 55 (0.10) CAC 154 (0.79) TCC 173 (0.31) Ile ATA 4(0.01) Thr ACG 184(0.38) 5 ATT 30 (0.08) ACA 24 (0.05) ATC 338 (0.91) ACT 21(0.05) ACC 249 (0.52) Lys AAG 284 (0.98) AAA 7 (0.02) Val GTG 308 (0.50) 10 GTA 9(0.01) Leu TTG 26 (0.04) GTT 35 (0.06) TTA 3 (0.00) GTC 262 (0.43) CTG 447 (0.61) CTA 20 (0.03) Trp TGG 107 (1.00) 15 CTT 45 (0.06) CTC 190 (0.26) Tyr TAT 10 (0.05) TAC 180 (0.95) Met ATG 191 (1.00) Stop TGA/TAG/TAA 20 20 WO 2014/151904 PCT/US2014/026644 Table 3: Preferred codon usage in Chlorella protothecoides TTC (Phe) TAC (Tyr) TGC (Cys) TGA (Stop) TGG (Trp) CCC (Pro) CAC (His) CGC (Arg) CTG (Leu) CAG (Gln) ATC (Ile) ACC (Thr) 5 GAC (Asp) TCC (Ser) ATG (Met) AAG (Lys) GCC (Ala) AAC (Asn) GGC (Gly) GTG (Val) GAG (Glu) Table 3a: Codon usage for Cuphea wrightii 10 UUU F 0.48 19.5 ( 52) UCU S 0.21 19.5 ( 52) UAU Y 0.45 6.4 ( 17) UGU C 0.41 10.5 ( 28) UUC F 0.52 21.3 ( 57) UCC S 0.26 23.6 ( 63) UAC Y 0.55 7.9 ( 21) UGC C 0.59 15.0( 40) UUA L 0.07 5.2 ( 14) UCA S 0.18 16.8 ( 45) UAA * 0.33 0.7 ( 2) UGA * 0.33 0.7 ( 15 2) UUG L 0.19 14.6( 39) UCG S 0.11 9.7 ( 26) UAG * 0.33 0.7 ( 2) UGG W 1.00 15.4 ( 41) CUU L 0.27 21.0 ( 56) CCU P 0.48 21.7 ( 58) CAU H 0.60 11.2 ( 30) CGU R 0.09 20 5.6 ( 15) CUC L 0.22 17.2 ( 46) CCC P 0.16 7.1 ( 19) CAC H 0.40 7.5 ( 20) CGC R 0.13 7.9 ( 21) CUA L 0.13 10.1 ( 27) CCA P 0.21 9.7( 26) CAA Q 0.31 8.6( 23) CGA R 0.11 6.7 ( 18) 25 CUG L 0.12 9.7 ( 26) CCG P 0.16 7.1 ( 19) CAG Q 0.69 19.5 ( 52) CGG R 0.16 9.4 ( 25) 21 WO 2014/151904 PCT/US2014/026644 AUU 10.44 22.8 ( 61) ACU T 0.33 16.8 ( 45) AAU N 0.66 31.4 ( 84) AGU S 0.18 16.1 ( 43) AUC I 0.29 15.4 ( 41) ACC T 0.27 13.9 ( 37) AAC N 0.34 16.5 ( 44) AGC S 0.07 6.0 5 ( 16) AUA 10.27 13.9 ( 37) ACA T 0.26 13.5 ( 36) AAA K 0.42 21.0 ( 56) AGA R 0.24 14.2 ( 38) AUG M 1.00 28.1 ( 75) ACG T 0.14 7.1 ( 19) AAG K 0.58 29.2 ( 78) AGG R 0.27 16.1 ( 43) 10 GUIJ V 0.28 19.8 ( 53) GCU A 0.35 31.4 ( 84) GAU D 0.63 35.9 ( 96) GGU G 0.29 26.6( 71) GUC V 0.21 15.0 ( 40) GCC A 0.20 18.0 ( 48) GAC D 0.37 21.0 ( 56) GGC G 0.20 18.0( 48) 15 GUA V 0.14 10.1 ( 27) GCA A 0.33 29.6 ( 79) GAA E 0.41 18.3 ( 49) GGA G 0.35 31.4( 84) GUG V 0.36 25.1 ( 67) GCG A 0.11 9.7( 26) GAG E 0.59 26.2( 70) GGG G 0.16 14.2 ( 38) Table 3b: Codon usage for Arabidopsis 20 UUU F 0.51 21.8 (678320) UCU S 0.28 25.2 (782818) UAU Y 0.52 14.6 (455089) UGU C 0.60 10.5 (327640) UUC F 0.49 20.7 (642407) UCC S 0.13 11.2 (348173) UAC Y 0.48 13.7 (427132) UGC C 0.40 7.2 (222769) UUA L 0.14 12.7 (394867) UCA S 0.20 18.3 (568570) UAA * 0.36 0.9 (29405) UGA * 25 0.44 1.2 ( 36260) UUG L 0.22 20.9 (649150) UCG S 0.10 9.3 (290158) UAG * 0.20 0.5 (16417) UGG W 1.00 12.5 (388049) 22 WO 2014/151904 PCT/US2014/026644 CUU L 0.26 24.1 (750114) CCU P 0.38 18.7 (580962) CAU H 0.61 13.8 (428694) CGU R 0.17 9.0 (280392) CUC L 0.17 16.1 (500524) CCC P 0.11 5.3 (165252) CAC H 0.39 8.7 (271155) CGC R 0.07 3.8 (117543) 5 CUA L 0.11 9.9 (307000) CCA P 0.33 16.1 (502101) CAA Q 0.56 19.4 (604800) CGA R 0.12 6.3 (195736) CUG L 0.11 9.8 (305822) CCG P 0.18 8.6 (268115) CAG Q 0.44 15.2 (473809) CGG R 0.09 4.9 (151572) 10 AUU 10.41 21.5 (668227) ACU T 0.34 17.5 (544807) AAU N 0.52 22.3 (693344) AGU S 0.16 14.0 (435738) AUC I 0.35 18.5 (576287) ACC T 0.20 10.3 (321640) AAC N 0.48 20.9 (650826) AGC S 0.13 11.3 (352568) AUA I 0.24 12.6 (391867) ACA T 0.31 15.7 (487161) AAA K 0.49 30.8 (957374) AGA R 15 0.35 19.0 (589788) AUG M 1.00 24.5 (762852) ACG T 0.15 7.7 (240652) AAG K 0.51 32.7 (1016176) AGG R 0.20 11.0 (340922) GUU V 0.40 27.2 (847061) GCU A 0.43 28.3 (880808) GAU D 0.68 36.6 (1139637) GGU 20 G 0.34 22.2 (689891) GUC V 0.19 12.8 (397008) GCC A 0.16 10.3 (321500) GAC D 0.32 17.2 (535668) GGC G 0.14 9.2 (284681) GUA V 0.15 9.9 (308605) GCA A 0.27 17.5 (543180) GAA E 0.52 34.3 (1068012) GGA G 0.37 24.2 (751489) 25 GUG V 0.26 17.4 (539873) GCG A 0.14 9.0 (280804) GAG E 0.48 32.2 (1002594) GGG G 0.16 10.2 (316620) 23 WO 2014/151904 PCT/US2014/026644 Host Cells [0050] The host cell can be a single cell (e.g., microalga, bacteria, yeast) or part of a multicellular organism such as a plant or fungus. Methods for expressing Fatb genes in a plant are given in 5,850,022; 5,723,761; 5,639,790; 5,807,893; 5 5,455,167; 5,654,495; 5,512,482;5,298,421;5,667,997; and 5,344,771; 5,304,481, or can be accomplished using other techniques generally known in plant biotechnology. Engineering of oleaginous microbes including those of Chlorophyta is disclosed in W02010/063032, W02011,150411, and W02012/106560 and in the examples below. 10 [00511 Examples of oleaginous host cells include plant cells and microbial cells having a type II fatty acid biosynthetic pathway, including plastidic oleaginous cells such as those of oleaginous algae. Specific examples of microalgal cells include heterotrophic or obligate heterotrophic microalgae of the phylum Chlorophtya, the class Trebouxiophytae, the order Chlorellales, or the family Chlorellacae. Examples 15 of oleaginous microalgae are provided in Published PCT Patent Applications W02008/151149, W02010/06032, W02011/150410, and W02011/150411, including species of Chlorella and Prototheca, a genus comprising obligate heterotrophs. The oleaginous cells can be, for example, capable of producing 25, 30, 40, 50, 60, 70, 80, 85, or about 90% oil by cell weight, ±5%. Optionally, the oils 20 produced can be low in DHA or EPA fatty acids. For example, the oils can comprise less than 5%, 2 %, or 1% DHA and/or EPA. The above-mentioned publications also disclose methods for cultivating such cells and extracting oil, especially from microalgal cells; such methods are applicable to the cells disclosed herein and incorporated by reference for these teachings. When microalgal cells are used they 25 can be cultivated autotrophically (unless an obligate heterotroph) or in the dark using a sugar (e.g., glucose, fructose and/or sucrose). In any of the embodiments described herein, the cells can be heterotrophic cells comprising an exogenous invertase gene so as to allow the cells to produce oil from a sucrose feedstock. Alternately, or in addition, the cells can metabolize xylose from cellulosic feedstocks. For example, the 30 cells can be genetically engineered to express one or more xylose metabolism genes such as those encoding an active xylose transporter, a xylulose-5 -phosphate transporter, a xylose isomerase, a xylulokinase, a xylitol dehydrogenase and a xylose 24 WO 2014/151904 PCT/US2014/026644 reductase. See W02012/154626, "GENETICALLY ENGINEERED MICROORGANISMS THAT METABOLIZE XYLOSE", published Nov 15, 2012. Oils and Related Products [0052] The oleaginous cells express one or more exogenous genes encoding 5 fatty acid biosynthesis enzymes. As a result, some embodiments feature natural oils that were not obtainable from a non-plant or non-seed oil, or not obtainable at all. [0053] The oleaginous cells produce a storage oil, which is primarily triacylglyceride and may be stored in storage bodies of the cell. A raw oil may be obtained from the cells by disrupting the cells and isolating the oil. W02008/151149, 10 W02010/06032, W02011/150410, and W02011/1504 disclose heterotrophic cultivation and oil isolation techniques. For example, oil may be obtained by cultivating, drying and pressing the cells. The oils produced may be refined, bleached and deodorized (RBD) as known in the art or as described in W02010/120939. The raw or RBD oils may be used in a variety of food, chemical, and industrial products or 15 processes. After recovery of the oil, a valuable residual biomass remains. Uses for the residual biomass include the production of paper, plastics, absorbents, adsorbents, as animal feed, for human nutrition, or for fertilizer. [0054] Where a fatty acid profile of a triglyceride (also referred to as a "triacylglyceride" or "TAG") cell oil is given here, it will be understood that this 20 refers to a nonfractionated sample of the storage oil extracted from the cell analyzed under conditions in which phospholipids have been removed or with an analysis method that is substantially insensitive to the fatty acids of the phospholipids (e.g. using chromatography and mass spectrometry). The oil may be subjected to an RBD process to remove phospholipids, free fatty acids and odors yet have only minor or 25 negligible changes to the fatty acid profile of the triglycerides in the oil. Because the cells are oleaginous, in some cases the storage oil will constitute the bulk of all the TAGs in the cell. [00551 The stable carbon isotope value 613C is an expression of the ratio of 13C/12C relative to a standard (e.g. PDB, carbonite of fossil skeleton of Belemnite 30 americana from Peedee formation of South Carolina). The stable carbon isotope value 613C (0/00) of the oils can be related to the 613C value of the feedstock used. 25 WO 2014/151904 PCT/US2014/026644 In some embodiments, the oils are derived from oleaginous organisms heterotrophically grown on sugar derived from a C4 plant such as corn or sugarcane. In some embodiments the 613C (0/00) of the oil is from -10 to -17 0/00 or from -13 to -16 0/00. 5 [0056] The oils produced according to the above methods in some cases are made using a microalgal host cell. As described above, the microalga can be, without limitation, fall in the classification of Chlorophyta, Trebouxiophyceae , Chlorellales, Chlorellaceae, or Chlorophyceae. It has been found that microalgae of Trebouxiophyceae can be distinguished from vegetable oils based on their sterol 10 profiles. Oil produced by Chlorella protothecoides was found to produce sterols that appeared to be brassicasterol, ergosterol, campesterol, stigmasterol, and p-sitosterol, when detected by GC-MS. However, it is believed that all sterols produced by Chlorella have C24p stereochemistry. Thus, it is believed that the molecules detected as campesterol, stigmasterol, and p-sitosterol, are actually 22,23 15 dihydrobrassicasterol, proferasterol and clionasterol, respectively. Thus, the oils produced by the microalgae described above can be distinguished from plant oils by the presence of sterols with C24p stereochemistry and the absence of C24a stereochemistry in the sterols present. For example, the oils produced may contain 22, 23-dihydrobrassicasterol while lacking campesterol; contain clionasterol, while 20 lacking in p-sitosterol, and/or contain poriferasterol while lacking stigmasterol. Alternately, or in addition, the oils may contain significant amounts of A 7 poriferasterol. [0057] In one embodiment, the oils provided herein are not vegetable oils. Vegetable oils are oils extracted from plants and plant seeds. Vegetable oils can be 25 distinguished from the non-plant oils provided herein on the basis of their oil content. A variety of methods for analyzing the oil content can be employed to determine the source of the oil or whether adulteration of an oil provided herein with an oil of a different (e.g. plant) origin has occurred. The determination can be made on the basis of one or a combination of the analytical methods. These tests include but are not 30 limited to analysis of one or more of free fatty acids, fatty acid profile, total triacylglycerol content, diacylglycerol content, peroxide values, spectroscopic properties (e.g. UV absorption), sterol profile, sterol degradation products, antioxidants (e.g. tocopherols), pigments (e.g. chlorophyll), d13C values and sensory 26 WO 2014/151904 PCT/US2014/026644 analysis (e.g. taste, odor, and mouth feel). Many such tests have been standardized for commercial oils such as the Codex Alimentarius standards for edible fats and oils. [0058] Sterol profile analysis is a particularly well-known method for determining the biological source of organic matter. Campesterol, b-sitosterol, and stigamsterol 5 are common plant sterols, with b-sitosterol being a principle plant sterol. For example, b-sitosterol was found to be in greatest abundance in an analysis of certain seed oils, approximately 64% in corn, 29% in rapeseed, 64% in sunflower, 74% in cottonseed, 26% in soybean, and 79% in olive oil (Gul et al. J. Cell and Molecular Biology 5:71-79, 2006). 10 [0059] Oil isolated from Prototheca moriformis strain UTEX1435 were separately clarified (CL), refined and bleached (RB), or refined, bleached and deodorized (RBD) and were tested for sterol content according to the procedure described in JAOCS vol. 60, no.8, August 1983. Results of the analysis are shown below (units in mg/100g): Refined, Refined & Sterol Crude Clarified bleached, & bleached deodorized 384 398 293 302 1 Ergosterol (56%) (55%) (50%) (50%) 5,22-cholestadien-24 14.6 18.8 14 15.2 2 methyl-3-ol 2r st erl-ol) (2.1%) (2.6%) (2.4%) (2.5%) (Brassicasterol) 24-methylcholest-5 3 en-3-ol (Campesterol or 10.7 11.9 10.9 10.8 22,23- (1.6%) (1.6%) (1.8%) (1.8%) dihydrobrassicasterol) 5,22-cholestadien-24 57.7 59.2 46.8 49.9 4 ethyl-3-ol (Stigmasterol orpoifrstro) (8.40%) (8.20%) (7.90%) (8.30%) or poriferasterol) 24-ethylcholest-5-en 9.64 9.92 9.26 10.2 5 3-ol ($3-Sitosterol or (1.4%) (1.4%) (1.6%) (1.7%) clionasterol) 6 Other sterols 209 221 216 213 Total sterols 685.64 718.82 589.96 601.1 15 [0060] These results show three striking features. First, ergosterol was found to be the most abundant of all the sterols, accounting for about 50% or more of the total 27 WO 2014/151904 PCT/US2014/026644 sterols. The amount of ergosterol is greater than that of campesterol, P-sitosterol, and stigmasterol combined. Ergosterol is steroid commonly found in fungus and not commonly found in plants, and its presence particularly in significant amounts serves as a useful marker for non-plant oils. Secondly, the oil was found to contain 5 brassicasterol. With the exception of rapeseed oil, brassicasterol is not commonly found in plant based oils. Thirdly, less than 2% P-sitosterol was found to be present. p-sitosterol is a prominent plant sterol not commonly found in microalgae, and its presence particularly in significant amounts serves as a useful marker for oils of plant origin. In summary, Prototheca moriformis strain UTEX1435 has been found to 10 contain both significant amounts of ergosterol and only trace amounts of p-sitosterol as a percentage of total sterol content. Accordingly, the ratio of ergosterol : p sitosterol or in combination with the presence of brassicasterol can be used to distinguish this oil from plant oils. [0061] In some embodiments, the oil content of an oil provided herein contains, as a 15 percentage of total sterols, less than 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1% p sitosterol. In other embodiments the oil is free from p-sitosterol. [0062] In some embodiments, the oil is free from one or more of p-sitosterol, campesterol, or stigmasterol. In some embodiments the oil is free from p-sitosterol, campesterol, and stigmasterol. In some embodiments the oil is free from campesterol. 20 In some embodiments the oil is free from stigmasterol. [0063] In some embodiments, the oil content of an oil provided herein comprises, as a percentage of total sterols, less than 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1% 24 ethylcholest-5-en-3-ol. In some embodiments, the 24-ethylcholest-5-en-3-ol is clionasterol. In some embodiments, the oil content of an oil provided herein 25 comprises, as a percentage of total sterols, at least 1 %, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10% clionasterol. [0064] In some embodiments, the oil content of an oil provided herein contains, as a percentage of total sterols, less than 20%, 15%, 10%, 5%, 4%, 3 %, 2%, or 1% 24 methylcholest-5-en-3-ol. In some embodiments, the 24-methylcholest-5-en-3-ol is 30 22, 23-dihydrobrassicasterol. In some embodiments, the oil content of an oil provided 28 WO 2014/151904 PCT/US2014/026644 herein comprises, as a percentage of total sterols, at least 1%, 2 %, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10% 22,23-dihydrobrassicasterol. [0065] In some embodiments, the oil content of an oil provided herein contains, as a percentage of total sterols, less than 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1% 5,22 5 cholestadien-24-ethyl-3-ol. In some embodiments, the 5, 22-cholestadien-24-ethyl-3 ol is poriferasterol. In some embodiments, the oil content of an oil provided herein comprises, as a percentage of total sterols, at least 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10% poriferasterol. [0066] In some embodiments, the oil content of an oil provided herein contains 10 ergosterol or brassicasterol or a combination of the two. In some embodiments, the oil content contains, as a percentage of total sterols, at least 5%, 10%, 20%, 25%, 35%, 40%, 45%, 50%, 55%, 60%, or 65% ergosterol. In some embodiments, the oil content contains, as a percentage of total sterols, at least 25% ergosterol. In some embodiments, the oil content contains, as a percentage of total sterols, at least 40% 15 ergosterol. In some embodiments, the oil content contains, as a percentage of total sterols, at least 5%, 10%, 20%, 25 %, 350%, 40%, 45%, 50%, 550%, 60%, or 65 % of a combination of ergosterol and brassicasterol. [0067] In some embodiments, the oil content contains, as a percentage of total sterols, at least 1%, 2%, 3%, 4% or 5% brassicasterol. In some embodiments, the oil content 20 contains, as a percentage of total sterols less than 10%, 9%, 8%, 7%, 6%, or 5% brassicasterol. [0068] In some embodiments the ratio of ergosterol to brassicasterol is at least 5:1, 10:1, 15:1, or 20:1. [0069] In some embodiments, the oil content contains, as a percentage of total sterols, 25 at least 50%, 10%, 20%, 25 %, 35 %, 40%, 45 %, 50%, 550%, 60%, or 65 % ergosterol and less than 20%, 15, 1% , 5%, 4%, 3%, 2%, or 1% p-sitosterol. In some embodiments, the oil content contains, as a percentage of total sterols, at least 25% ergosterol and less than 5% p-sitosterol. In some embodiments, the oil content further comprises brassicasterol. 30 [0070] Sterols contain from 27 to 29 carbon atoms (C27 to C29) and are found in all eukaryotes. Animals exclusively make C27 sterols as they lack the ability to further 29 WO 2014/151904 PCT/US2014/026644 modify the C27 sterols to produce C28 and C29 sterols. Plants however are able to synthesize C28 and C29 sterols, and C28/C29 plant sterols are often referred to as phytosterols. The sterol profile of a given plant is high in C29 sterols, and the primary sterols in plants are typically the C29 sterols b-sitosterol and stigmasterol. In 5 contrast, the sterol profile of non-plant organisms contain greater percentages of C27 and C28 sterols. For example the sterols in fungi and in many microalgae are principally C28 sterols. The sterol profile and particularly the striking predominance of C29 sterols over C28 sterols in plants has been exploited for determining the proportion of plant and marine matter in soil samples (Huang, Wen-Yen, Meinschein 10 W. G., "Sterols as ecological indicators"; Geochimica et Cosmochimia Acta. Vol 43. pp 739-745). [0071] In some embodiments the primary sterols in the microalgal oils provided herein are sterols other than b-sitosterol and stigmasterol. In some embodiments of the microalgal oils, C29 sterols make up less than 50%, 40%, 30%, 20%, 10%, or 5% 15 by weight of the total sterol content. [0072] In some embodiments the microalgal oils provided herein contain C28 sterols in excess of C29 sterols. In some embodiments of the microalgal oils, C28 sterols make up greater than 50%, 60%, 70%, 80%, 90%, or 95% by weight of the total sterol content. In some embodiments the C28 sterol is ergosterol. In some embodiments the 20 C28 sterol is brassicasterol. [0073] In embodiments of the present invention, oleaginous cells expressing one or more of the genes of Table 1 can produce an oil with at least 20, 40, 60 or 7 0% of C8, C1O, C12, C14 or C16 fatty acids. In a specific embodiment, the level of myristate (C 14:0) in the oil is greater than 30%. 25 [0074] Thus, in embodiments of the invention, there is a process for producing an oil, triglyceride, fatty acid, or derivative of any of these, comprising transforming a cell with any of the nucleic acids discussed herein. In another embodiment, the transformed cell is cultivated to produce an oil and, optionally, the oil is extracted. Oil extracted in this way can be used to produce food, oleochemicals or other 30 products. 30 WO 2014/151904 PCT/US2014/026644 [00751 The oils discussed above alone or in combination are useful in the production of foods, fuels and chemicals (including plastics, foams, films, etc). The oils, triglycerides, fatty acids from the oils may be subjected to C-H activation, hydroamino methylation, methoxy-carbonation, ozonolysis, enzymatic 5 transformations, epoxidation, methylation, dimerization, thiolation, metathesis, hydro alkylation, lactonization, or other chemical processes. [00761 After extracting the oil, a residual biomass may be left, which may have use as a fuel, as an animal feed, or as an ingredient in paper, plastic, or other product. For example, residual biomass from heterotrophic algae can be used in such 10 products. Example 1. Discovery of Novel FATB sequences [00771 Sequences of novel plant acyl-ACP thioesterases involved in seed-specific mid-chain (C8-C 16) fatty acid biosynthesis in higher plants were isolated. Seed-specific lipid production genes were isolated through direct 15 interrogation of RNA pools accumulating in oilseeds. Based on phylogenetic analysis, novel enzymes can be classified as members of FatB family of acyl-ACP thioesterases. [0078] Seeds of oleaginous plants were obtained from local grocery stores or requested through USDA ARS National Plant Germplasm System (NPGS) from 20 North Central Regional Plant Introduction Station (NCRIS) or USDA ARS North Central Soil Conservation Research Laboratory (Morris, MI). Dry seeds were homogenized in liquid nitrogen to powder, resuspended in cold extraction buffer containing 6-8M Urea and 3M LiCl and left on ice for a few hours to overnight at 4 0 C. The seed homogenate was passed through NucleoSpin Filters (Macherey-Nagel) 25 by centrifugation at 20,000g for 20 minutes in the refrigerated microcentrifuge (4 0 C). The resulting RNA pellets were resuspended in the buffer containing 20 mM Tris HCl, pH7.5, 0.50% SDS, 100 mM NaCl, 25 mM EDTA, 2% PVPP) and RNA was subsequently extracted once with Phenol-Chloroform-Isoamyl Alcohol (25:24:1, v/v) and once with chloroform. RNA was finally precipitated with isopropyl alcohol (0.7 30 Vol.) in the presence of 150 mM of Na Acetate, pH5.2, washed with 80% ethanol by centrifugation, and dried. RNA samples were treated with Turbo DNAse (Lifetech) and purified further using RNeasy kits (Qiagen) following manufacturers' protocols. 31 WO 2014/151904 PCT/US2014/026644 The resulting purified RNA samples were converted to pair-end cDNA libraries and subjected to next-generation sequencing (2x1OObp) using Illumina Hiseq 2000 platform. RNA sequence reads were assembled into corresponding seed transcriptomes using Trinity or Oases packages. Putative thioesterase-containg cDNA 5 contigs were identified by mining transcriptomes for sequences with homology to known thioesterases. These in silico identified putative thioesterase cDNAs have been further verified by direct reverse transcription PCR analysis using seed RNA and primer pairs targeting full-length thioesterase cDNAs. The resulting amplified products were cloned and sequenced de novo to confirm authenticity of identified 10 thioesterase genes. [00791 To interrogate evolutionary and functional relationship between novel acyl-ACP thioesterases and the members of two existing thioesterase classes (FatA and FatB), we performed a phylogenetic analysis using published full-length (Mayer and Shanklin, 2007) and truncated (THYME database) amino acid thioesterase 15 sequences. Novel proteins appear to group with known acyl-ACP FatB thioesterases involved in biosynthesis of C8-C16 fatty acids. Moreover, novel thioesterases appear to cluster into 3 predominant out-groups suggesting distinct functional similarity and evolutionary relatedness among members of each cluster. [0080] The amino acid sequences of the FatB genes follow are shown in Table 20 4. Table 4: Amino acid sequences of FatB genes: CuPSR23 FATB3 SEQ ID NO: 110 CuPSR23 FATB3b SEQ ID NO: 111 CwFATB3 SEQ ID NO: 112 CwFATB3a SEQ ID NO: 113 CwFATB3b SEQ ID NO: 114 CwFATB3c SEQ ID NO: 115 CwFATB4a SEQ ID NO: 116 CwFATB4a.1 SEQ ID NO: 117 CwFATB4a.2 SEQ ID NO: 118 CwFATB4a.3 SEQ ID NO: 119 32 WO 2014/151904 PCT/US2014/026644 CwFATB4b SEQ ID NO: 120 CwFATB4b.1 SEQ ID NO: 121 CwFATB5 SEQ ID NO: 122 CwFATB5a SEQ ID NO: 123 CwFATB5b SEQ ID NO: 124 CwFATB5c SEQ ID NO: 125 CwFATB5.1 SEQ ID NO: 126 CwFATB5.1a SEQ ID NO: 127 CcFATB2b SEQ ID NO: 128 CcFATB3 SEQ ID NO: 129 CcFATB3b SEQ ID NO: 130 CcFATB3c SEQ ID NO: 131 ChtFATBla SEQ ID NO: 132 ChtFATBla.1 SEQ ID NO: 133 ChtFATBla.2 SEQ ID NO: 134 ChtFATBla.3 SEQ ID NO: 135 ChtFATBla.4 SEQ ID NO: 136 ChtFATB1b SEQ ID NO: 137 ChtFATB2b SEQ ID NO: 138 ChtFATB2a SEQ ID NO: 139 ChtFATB2c SEQ ID NO: 140 ChtFATB2d SEQ ID NO: 141 ChtFATB2e SEQ ID NO: 142 ChtFATB2f SEQ ID NO: 143 ChtFATB2g SEQ ID NO: 144 ChtFATB2h SEQ ID NO: 145 ChtFATB3a SEQ ID NO: 146 ChtFATB3b SEQ ID NO: 147 ChtFATB3c SEQ ID NO: 148 ChtFATB3d SEQ ID NO: 149 ChtFATB3e SEQ ID NO: 150 ChtFATB3f SEQ ID NO: 151 33 WO 2014/151904 PCT/US2014/026644 ChtFATB3g SEQ ID NO: 152 ChsFATB1 SEQ ID NO: 153 ChsFATB2 SEQ ID NO: 154 ChsFatB2b SEQ ID NO: 155 ChsFatB2c SEQ ID NO: 156 ChsFatB2d SEQ ID NO: 157 Chs FATB3 SEQ ID NO: 158 ChsFatb3b SEQ ID NO: 159 ChsFatB3c SEQ ID NO: 160 ChsFATB3d SEQ ID NO: 161 ChsFATB3e SEQ ID NO: 162 ChsFATB3f SEQ ID NO: 163 ChsFATB3g SEQ ID NO: 164 ChsFATB3h SEQ ID NO: 165 ChsFATB3i SEQ ID NO: 166 ChsFATB3j SEQ ID NO: 167 ChsFATB3j: MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQV KANASARPKANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITT 5 VFVAAEKQWTMLDRKSKRPDMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGA DRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWVVTKMHIEV NRYPTWGDTIEVNTWVSESGKTGMGRDWLISDFHTGDILIRATSVCAMMNQ KTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRW NDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRQECGRDSVLESV 10 TAMDPSKEGDRSLYQHLLRLEDGTDIAKGRTKWRPKNAGKTSNGNSIS 34 WO 2014/151904 PCT/US2014/026644 Example 2. Cloning and fatty acid analysis of cells transformed with novel FATB genes [0081] In the example below, we detail the effect of expressing plant oilseed transcriptome-derived, heterologous thioesterases in the UTEX1435 5 (web.biosci.utexas.edu/utex/) strain, Strain A. [0082] As in Example 1, RNA was extracted from dried plant seeds and submitted for paired-end sequencing using the Illumina Hiseq 2000 platform. RNA sequence reads were assembled into corresponding seed transcriptomes using Trinity or Oases packages and putative thioesterase-containing cDNA contigs were identified 10 by mining transcriptomes for sequences with homology to known thioesterases. These in silico identified putative thioesterase cDNAs were verified by direct reverse transcription PCR analysis using seed RNA and primer pairs targeting full-length thioesterase cDNAs. The resulting amplified products were cloned and sequenced de novo to confirm authenticity of identified thioesterase genes and to identify sequence 15 variants arising from expression of different gene alleles or diversity of sequences within a population of seeds. The resulting amino acid sequences were subjected to phylogenetic analysis using published full-length (Mayer and Shanklin, 2007) and truncated (THYME database) FatB sequences. The thioesterases that clustered with acyl-ACP FatB thioesterases, which are involved in biosynthesis of C8-C16 fatty 20 acids, were pursued. Construction of Transforming Vectors Expressing Acyl-ACP FatB Thioesterases [0083] 27 putative acyl-ACP FatB thioesterases from the species Cinnamomum camphora, Cuphea hyssopifolia, Cuphea PSR23, Cuphea wrightii, Cuphea heterophylla, and Cuphea viscosissima were synthesized in a codon 25 optimized form to reflect Prototheca moriformis (UTEX 1435) codon usage. Of the 27 genes synthesized, 24 were identified by our transcriptome sequencing efforts and the 3 genes from Cuphea viscosissima, were from published sequences in GenBank. [0084] Transgenic strains were generated via transformation of the base strain Strain A (Prototheca moriformis, derived from UTEX 1435 by classical mutation and 30 screening for high oil production) with a construct encoding 1 of the 27 FatB thioesterases. The construct pSZ2760 encoding Cinnamomum camphora (Cc) FATB~b is shown as an example, but identical methods were used to generate each of 35 WO 2014/151904 PCT/US2014/026644 the remaining 26 constructs encoding the different respective thioesterases. Construct pSZ2760 can be written as 6S::CrTUB2:ScSUC2:CvNR::PmAMT3:CcFATB1b:CvNR::6S. The sequence of the transforming DNA is provided in Table 5 (pSZ2760). The relevant restriction sites in 5 the construct from 5'-3', BspQ1, KpnI, AscI, MfeI, EcoRI, Spel, XhoI, Sac, BspQ1, respectively, are indicated in lowercase, bold, and underlined. BspQ1 sites delimit the 5' and 3' ends of the transforming DNA. Bold, lowercase sequences at the 5' and 3' end of the construct represent genomic DNA from UTEX 1435 that target integration to the 6S locus via homologous recombination. Proceeding in the 5' to 3' direction, 10 the selection cassette has the C. reinhardtii f-tubulin promoter driving expression of the S. cerevisiae gene SUC2 (conferring the ability to grow on sucrose) and the Chlorella vulgaris Nitrate Reductase (NR) gene 3' UTR. The promoter is indicated by lowercase, boxed text. The initiator ATG and terminator TGA for ScSUC2 are indicated by bold, uppercase italics, while the coding region is indicated with 15 lowercase italics. The 3' UTR is indicated by lowercase underlined text. The spacer region between the two cassettes is indicated by upper case text. The second cassette containing the codon optimized CcFATB~b gene (Table 5; pSZ2760) from Cinnamomum camphora is driven by the Prototheca moriformis endogenous AMT3 promoter, and has the Chlorella vulgaris Nitrate Reductase (NR) gene 3' UTR. In 20 this cassette, the AMT3 promoter is indicated by lowercase, boxed text. The initiator ATG and terminator TGA for the CcFATB~b gene are indicated in bold, uppercase italics, while the coding region is indicated by lowercase italics and the spacer region is indicated by upper case text. The 3' UTR is indicated by lowercase underlined text. The final construct was sequenced to ensure correct reading frame and targeting 25 sequences. Table 5: pSZ2760 Transforming construct gctcttcgccgccgccactcctgctcgagcgcgcccgcgcgtgcgccgccagcgccttggccttttcgccgcgctcgtgc gcgtcgctgatgtccatcaccaggtccatgaggtctgccttgcgccggctgagccactgcttcgtccgggcggccaagag 30 gagcatgagggaggactcctggtccagggtcctgacgtggtcgcggctctgggagcgggccagcatcatctggctctgc cgcaccgaggccgcctccaactggtcctccagcagccgcagtcgccgccgaccctggcagaggaagacaggtgaggg gggtatgaattgtacagaacaaccacgagccttgtctaggcagaatccctaccagtcatggctttacctggatgacggcctg 36 WO 2014/151904 PCT/US2014/026644 cgaacagctgtccagcgaccctcgctgccgccgcttctcccgcacgcttctttccagcaccgtgatggcgcgagccagcg ccgcacgctggcgctgcgcttcgccgatctgaggacagtcggggaactctgatcagtctaaacccccttgcgcgttagtgtt gccatcctttgcagaccggtgagagccgacttgttgtgcgccaccccccacaccacctcctcccagaccaattctgtcacct ttttggcgaaggcatcggcctcggcctgcagagaggacagcagtgcccagccgctgggggttggcggatgcacgctca 5 taccctttcttgegetatgacacttccagcaaaagtagggetgegagacggttccgegetgcatgcaac ccgatgatgcttcgaccccccgaagctccttcggggctgcatgggegctccgatgccgctccagggcgageetttta atagccaggcecccegattgcaaagacattatagcgagctaccaaagccatattcaaacacctagatcactaccacttctac caggccactcgagcttgtgategcactecgctaagggggcetettectettegtttcagtcacaacccgcaaac gec gccA TGctgctgcaggccttcctgttcctgctggccggcttcgccgccaagatcagcgcctccatgacgaacgagac 10 gtccgaccgccccctggtgcacttcacccccaacaagggctggatgaacgaccccaacggcctgtggtacgacgag aaggacgccaagtggcacctgtacttccagtacaacccgaacgacaccgtctgggggacgcccttgttctggggcca cgccacgtccgacgacctgaccaactgggaggaccagcccatcgccatcgccccgaagcgcaacgactccggcgc cttctccggctccatggtggtggactacaacaacacctccggcttcttcaacgacaccatcgacccgcgccagcgctgc gtggccatctggacctacaacaccccggagtccgaggagcagtacatctcctacagcctggacggcggctacaccttc 15 accgagtaccagaagaaccccgtgctggccgccaactccacccagttccgcgacccgaaggtcttctggtacgagcc ctcccagaagtggatcatgaccgcggccaagtcccaggactacaagatcgagatctactcctccgacgacctgaagt cctggaagctggagtccgcgttcgccaacgagggcttcctcggctaccagtacgagtgccccggcctgatcgaggtcc ccaccgagcaggaccccagcaagtcctactgggtgatgttcatctccatcaaccccggcgccccggccggcggctcct tcaaccagtacttcgtcggcagcttcaacggcacccacttcgaggccttcgacaaccagtcccgcgtggtggacttcgg 20 caaggactactacgccctgcagaccttcttcaacaccgacccgacctacgggagcgccctgggcatcgcgtgggcctc caactgggagtactccgccttcgtgcccaccaacccctggcgctcctccatgtccctcgtgcgcaagttctccctcaaca ccgagtaccaggccaacccggagacggagctgatcaacctgaaggccgagccgatcctgaacatcagcaacgccg gcccctggagccggttcgccaccaacaccacgttgacgaaggccaacagctacaacgtcgacctgtccaacagcac cggcaccctggagttcgagctggtgtacgccgtcaacaccacccagacgatctccaagtccgtgttcgcggacctctcc 25 ctctggttcaagggcctggaggaccccgaggagtacctccgcatgggcttcgaggtgtccgcgtcctccttcttcctgga ccgcgggaacagcaaggtgaagttcgtgaaggagaacccctacttcaccaaccgcatgagcgtgaacaaccagcc cttcaagagcgagaacgacctgtcctactacaaggtgtacggcttgctggaccagaacatcctggagctgtacttcaac gacggcgacgtcgtgtccaccaacacctacttcatgaccaccgggaacgccctgggctccgtgaacatgacgacggg ggtggacaacctgttctacatcgacaagttccaggtgcgcgaggtcaagTGAcaattggcagcagcagctcggatag 30 tatcgacacactctagacgctggtcgtgtgatagactgttgccgccacacttgctgccttgacctgtgaatatccetgccgctt ttatcaaacagcctcagtgtgtttgatcttgtgtgtac gcgcttttgc gagttgctagctgcttgtgctatttgcgaataccacccc cagcatccccttccctcgtttcatatcgcttgcatcccaaccgcaacttatctacgctgtcctgctatccctcagc gctgctcct gctcctgctcactgcccctcgcacagccttggtttggctccgcctgtattctcctggtactgcaacctgtaaaccagcactg 37 WO 2014/151904 PCT/US2014/026644 caatg4ctg4atgcacgggaag4tag4tgggatggaacacaaatggaAAGCTGTATAGGGATAAgaattc ccgacaggac cctcaaaggtctcttatccctggccggcagtcgttgctgctgctttagtattccgc accctgattttgge tcttattttgge tggaaaecgtggCgCCCg agccggccgcggcgatgcg tgceccacg gctgCCggaatccaaggaggcaagagcgcccggteagttgaaggetttae e eaagtaea e eee e 5 eggtge tggtgaattgae tgeaggteetgetgaagtteeteeaee eeteaeeagce aeaaageaeet a aggtee teateeaetetaaagaaete aetae aeetaetgatggeeetagattetteateaaaaaegc eetaaeaet eeecaggattgaaaeteeetgaaggaeeaeeaggeeectgagttgtteetteeeeee tge aget ea e ectgtaeetgtate aggetgge aaaatagette ttgeteaggteatggagtgeaggaeagetetae eecaaeaate eaeaatteatgteaagetaateagetattteetetteae agetgtaattgteeeaaaattetg tetaeegg 10 gtgateette tgtacggeeetteeeteaaeeetaggtatgeCgceatge teccc e aaeteCgCg aggccg ggtttggacggccgtec aaatgcagttgeacee gatge tggeaeettttttgCgataatttatgeaaace ctgeaaaattetggetetgtCgeaaeeetaggateagCggCgtagattte taateatte teetgatgggagetace aetaeeetaatateagcccgactgectgacgecagCgteeaettttgtgeacacattecatte tgeeeaagaeatet tgCgaagCgteeccagttae eteacetgttteee aecteettactgttetgte acagage ecca c 15 jj aekg!ATGgccaccacctccctggcctccgccttctgctccatgaaggccgtgatgctggcccgcgacg gccgcggcctgaagccccgctcctccgacctgcagctgcgcgccggcaacgcccagacctccctgaagatgatcaac ggcaccaagttctcctacaccgagtccctgaagaagctgcccgactggtccatgctgttcgccgtgatcaccaccatctt ctccgccgccgagaagcagtggaccaacctggagtggaagcccaagcccaaccccccccagctgctggacgacca cttcggcccccacggcctggtgttccgccgcaccttcgccatccgctcctacgaggtgggccccgaccgctccacctcc 20 atcgtggccgtgatgaaccacctgcaggaggccgccctgaaccacgccaagtccgtgggcatcctgggcgacggctt cggcaccaccctggagatgtccaagcgcgacctgatctgggtggtgaagcgcacccacgtggccgtggagcgctacc ccgcctggggcgacaccgtggaggtggagtgctgggtgggcgcctccggcaacaacggccgccgccacgacttcct ggtgcgcgactgcaagaccggcgagatcctgacccgctgcacctccctgtccgtgatgatgaacacccgcacccgcc gcctgtccaagatccccgaggaggtgcgcggcgagatcggccccgccttcatcgacaacgtggccgtgaaggacga 25 ggagatcaagaagccccagaagctgaacgactccaccgccgactacatccagggcggcctgaccccccgctggaa cgacctggacatcaaccagcacgtgaacaacatcaagtacgtggactggatcctggagaccgtgcccgactccatctt cgagtcccaccacatctcctccttcaccatcgagtaccgccgcgagtgcacccgcgactccgtgctgcagtccctgacc accgtgtccggcggctcctccgaggccggcctggtgtgcgagcacctgctgcagctggagggcggctccgaggtgct gcgcgccaagaccgagtggcgccccaagctgtccttccgcggcatctccgtgatccccgccgagtcctccgtgatgga 30 ctacaaggaccacgacggcgactacaaggaccacgacatcgactacaaggacgacgacgacaaglGAte a ggeagecagecaetgatatatcgaaattggagetgtgttatgatttgee gcacacttgctgeecttg4 acctg4tgaatatccctgeege4ttttatcaaacagctcag4tg4tgtttg4atcttgtgtgtacegegettttgeg ag4ttgectagetget 38 WO 2014/151904 PCT/US2014/026644 gtgctatttgcgaataccacccccagcatccccttccctcgtttcatatcgcttcatcccaacccaacttatctac gctgtcc tgctatccctcagcgctgctcctgctcctgctcactgcccctcgcacagccttggtttggctcgccttattctcctagtact gcaacctgtaaaccagcactgcaatgctgatgcac gggaatataggataggaacacaaatagaAAGCTGTAT AGGGATAACAGGGTAATgagctcttgttttccagaaggagttgctccttgagcctttcattctcagcctcgata 5 acctccaaagccgctctaattgtggagggggttcgaatttaaaagcttggaatgttggttcgtgcgtctggaacaagcccag acttgttgctcactgggaaaaggaccatcagctccaaaaaacttgccgctcaaaccgcgtacctctgctttcgcgcaatctgc cctgttgaaatcgccaccacattcatattgtgacgcttgagcagtctgtaattgcctcagaatgtggaatcatctgccccctgtg cgagcccatgccaggcatgtcgcgggcgaggacacccgccactcgtacagcagaccattatgctacctcacaatagttca taacagtgaccatatttctcgaagctccccaacgagcacctccatgctctgagtggccaccccccggccctggtgcttgcg 10 gagggcaggtcaaccggcatggggctaccgaaatccccgaccggatcccaccacccccgcgatgggaagaatctctcc ccgggatgtgggcccaccaccagcacaacctgctggcccaggcgagcgtcaaaccataccacacaaatatccttggcat cggccctgaattccttctgccgctctgctacccggtgcttctgtccgaagcaggggttgctagggatcgctccgagtccgca aacccttgtcgcgtggcggggcttgttcgagcttgaagagc 15 [00851 Constructs encoding the identified heterologous FatB genes, such as CcFA TB b from pSZ2760 in Table 6, were transformed into Strain A, and selected for the ability to grow on sucrose. Transformations, cell culture, lipid production and fatty acid analysis were all carried out as previously described. After cultivating on sucrose under low nitrogen conditions to accumulate oil, fatty acid profiles were 20 determined by FAME-GC. The top performer from each transformation, as judged by the ability to produce the highest level of midchain fatty acids, is shown in Table 4. 39 WO 2014/151904 PCT/US2014/026644 Table 6: Alteration of Fatty Acid Profiles in S3150 upon Expression of Heterologous FatB Thioesterases FA profile of top performer from each transformation (%; primary lipid in Strain A background) Species Gene Name SZ Plasmid Strain C8:0 C10:0 C12:0 C14:0 C16:0 C18:0 C18:1 C18:2 C18:3a Cinnamomum camphora CcFATB1b pSZ2760 A; T526; D1670-13 0 0 1 15 26 2 46 9 1 Cinnamomum camphora CcFATB4 pSZ2756 A; T525; D1666-31 0 1 33 4 7 2 41 10 1 Cinnamomum camphora CcFATB3 pSZ2755 A; T525; D1665-4 0 0 0 3 44 3 41 8 0 Cuphea hyssopifolia ChsFATB1 pSZ2778 A; T535; D1639-30 0 0 0 2 22 4 63 8 1 Cuphea hyssopifolia ChsFATB2 pSZ2796 A; T537; D1700-46 0 0 0 6 53 3 32 6 0 Cuphea hyssopifolia ChsFATB2b pSZ2792 A; T537; D166-9 0 0 0 5 26 2 56 9 1 Cuphea hyssopifolia ChsFATB3 pSZ2797 A; T537; D1701-48 0 0 8 34 27 2 24 5 1 Cupheahyssopifolia ChsFATB3b pSZ2795 A;T537; D1699-1 0 0 7 29 27 1 28 6 1 CupheaPSR23 CuPSR23FATB3 pSZ2793 A;T537;D1697-13 0 1 0 2 24 3 61 8 1 Cuphea wrighti CwFATB3 pSZ2751 A;T525; D1661-22 0 2 17 9 19 2 41 8 1 Cup hea wrighti CwFATB4a pSZ2752 A;T525; D1662-30 0 0 0 4 48 3 36 7 1 Cuphea wrighti CwFATB4b pSZ2753 A;T525; D1663-29 0 0 0 5 52 3 32 6 1 Cuphea wrighti CwFATB5 pSZ2754 A;T525; D1664-39 0 0 0 3 27 3 57 7 1 Cuphea heterophylla ChtFATBla pSZ2757 A; T525; D1667-19 0 0 5 18 27 2 39 7 1 Cuphea heterophylla ChtFATB1b pSZ2773 A; T535; D1685-29 0 0 2 7 27 3 53 8 1 Cuphea heterophylla ChtFATB2b pSZ2780 A; T535; D1691-8 0 0 0 2 25 3 61 8 1 Cuphea heterophy//a ChtFATB2a pSZ2774 A; T537; D1702-24 0 0 0 2 27 3 59 6 0 Cuphea heterophy//a ChtFATB2c pSZ2758 A; T525; D1668-22 0 0 3 2 23 3 58 7 1 Cuphea heterophy//a ChtFATB2d pSZ2759 A; T526; D1669-19 0 0 4 4 23 3 54 9 1 Cuphea heterophy//a ChtFATB2e pSZ2775 A; T535; D16-6-23 0 1 2 3 24 3 57 8 1 Cuphea heterophy//a ChtFATB2f pSZ2777 A; T535; D168- 33 0 0 0 2 28 3 57 8 1 Cuphea heterophy//a ChtFATB2g pSZ2794 A; T537; D1698-19 0 0 0 2 22 3 62 9 1 Cuphea heterophy//a ChtFATB3a pSZ2776 A; T535; D1687-23 0 0 0 5 47 4 37 7 1 Cuphea heterophyla ChtFATB3b pSZ2779 A; T535; D1690- 311 0 0 0 6 49 5 32 7 0 Cuphea viscosissima CvisFATB1 pSZ2810 A; T540; D1711-30 0 1 0 2 24 3 60 8 0 Cuphea viscosissima CvisFATB2 pSZ2817 A; T547; D1718-1 0 0 0 4 51 2 36 6 0 Cuphea viscosissima CvisFATB3 pSZ2791 A; T537; D1695-1 0 0 0 8 28 2 52 8 1 A(parentstrain): 0 0 0 2 28 3 58 7 0 [0086] Many of the acyl-ACP FatB thioesterases were found to exhibit 5 midchain activity when expressed in Prototheca moriformis. For example, expression of CcFA TBIb causes an increase in myristate levels from 2% of total fatty acids in the parent, Strain A, to ~15% in the D1670-13 primary transformant. Other examples include CcFATB4, which exhibits an increase in laurate levels from 0% in Strain A to ~33%, and ChsFATB3, which exhibits an increase in myristate levels to ~34%. 10 Although some of the acyl-ACP thioesterases did not exhibit dramatic effects on midchain levels in the current incarnation, efforts will likely develop to optimize some of these constructs. [00871 Sequences of the Heterologous Acyl-ACP Thioesterases Identified and Transformed into P. moriformis (UTEX 1435) 15 [00881 A complete listing of relevant sequences for the transforming constructs, such as the deduced amino acid sequence of the encoded acyl-ACP thioesterase, the native CDS coding sequence, the Prototheca moriformis codon 40 WO 2014/151904 PCT/US2014/026644 optimized coding sequence, and the nature of the sequence variants examined, is provided as SEQ ID NOS: 1-78. Example 3. Discovery and Cloning of Additional FATB genes Additional FATB genes were obtained from seeds as described above. The species 5 and number of FatB genes identified were: Species Accession Novel FatB Number Thioesterase Genes Cuphea calcarata 534665 1 Cuphea painteri 288248 1 Cuphea hookeriana 534896 1 Cuphea avigera var. Ames 1 pulcherrima 17868 Cuphea paucipetala 534877 1 Cuphea procumbens 534881 3 Cupheaignea 534773 1 [0089] The thioesterases that clustered with acyl-ACP FatB thioesterases, which are involved in biosynthesis of C8-C16 fatty acids, were pursued. The native, 10 putative plastid-targeting transit peptide sequence is indicated by underlining. [0090] Construction of Transforming Vectors Expressing Acyl-ACP FatB Thioesterases. The nine putative Acyl-ACP FatB Thioesterases from the species Cuphea calcarata, Cuphea painter, Cuphea hookeriana, Cuphea avigera var. pulcherrima, Cuphea paucipetala, Cuphea procumbens, and Cuphea ignea were 15 synthesized in a codon-optimized form to reflect UTEX 1435 codon usage. In contrast to the previous example, the new Acyl-ACP FatB thioesterases were synthesized with a modified transit peptide from Chlorella protothecoides (Cp) in place of the native transit peptide. The modified transit peptide derived from the CpSAD1 gene, "CpSADltp trimmed", was synthesized as an in-frame, N-terminal 41 WO 2014/151904 PCT/US2014/026644 fusion to the FatB acyl-ACP thioesterases in place of the native transit peptide; the resulting sequences are listed below. The novel FatB genes were cloned into Prototheca moriformis as described above. Constructs encoding heterologous FatB genes were transformed into strain S6165 (a descendant of S3150/Strain A) and 5 selected for the ability to grow on sucrose. Transformations, cell culture, lipid production and fatty acid analysis were all carried out as previously described. The results for the nine novel FatB acyl-ACP thioesterases are displayed in the table immediately below. 42 WO 2014/151904 PCT/US2014/026644 m E- - -- Uo r j r j j j E 0 o , m U u R~ m 0: 0C E 0 o- 0 ooro o T - - - - 0o0 E .: 0o r t G ,r D r n 0* 0. u E 0 z wc 2DC D Dm F r_ < n F- Co F o U 0 CL m C~ 00Q c j CuN 0 0 ~ 0N C Z Q -Q: - U o- -z: tz o- o- 0 z tz z tz Uj Uj Uj U UjQ:Q j j Q . Q 0. [09] O atclrnt r:CaFTB1 hc xiis1%C1:0ad8 C8001 Oftt paricla notels are: aiAB, which exhibits : n 1%0 C12:0 fandt8y 43 WO 2014/151904 PCT/US2014/026644 acid levels; CigneaFATB1, which exhibits 8 % C10:0 and 1% C12:0 fatty acid levels; CcalcFATBI, which exhibits 18% C14:0 and 12% C12:0 levels; and CaFATBI, which exhibits 22% C8:0 and 9% C10:0 fatty acid levels. [0092] CaFATBI, which exhibits high C8:0 and C10:0 levels, is of particular 5 interest. CaFATBI arose from two separate contigs that were assembled from the Cupha avigera var. pulcherrima transcriptome, S 17_Cavig trinity_7406 and S17_Cavig trinity_7407. Although the two partial contigs exhibit only 17 nucleotides of overlap, we were able to assemble a putative full length transcript encoding CaFATB 1 from the two contigs and then subsequently confirm the 10 existence of the full-length transcript by direct reverse transcription PCR analysis using seed RNA and primer pairs targeting the full-length CaFATB 1 thioesterase cDNA. Tjellstrom et al. (2013) discloses the expression of a newly identified fatty acyl-ACP thioesterase from Cuphea pulcherrima that they named "CpuFATB3" (Genbank accession number KC675178). The coding sequence of CpuFATB3 is 15 100% identical to the CaFATB 1 gene we identified and contains one nucleotide difference in the RNA sequence outside the predicted coding region. Tjellstrom et al. (2013) showed that CpuFATB3 produces an average of 4.8% C8:0 when expressed in Arabidopsis, and further requires deletion of two acyl-ACP synthetases, AAE15/16, to produce an average of 9.2% C8:0 with a maximum level of ~12% C8.0. The 20 CaFATBI gene we identified was codon-optimized for expression in UTEX1435 and generated as a CpSAD ltp-trimmed transit peptide fusion before introduction into S6165. The CpSADltp trimmed:CaFATB1 gene produces an average C8:0 level of 14% and a maximum level of 22% C8:0 without requiring the deletion of endogenous acyl-ACP synthetases. 25 [0093] Table 7. Amino Acid Sequences of Additional Novel FatB Acyl-ACP Thioesterases. In the appended sequence listings, the native, putative plastid-targeting transit peptide sequence is underlined: FatB Sequence ID NO: CcalcFATB1 (Cuphea calcarata FATB1) SEQ ID NO: 168 ChookFATB4 (Cuphea hookeriana FATB4) SEQ ID NO: 169 CaFATB 1 (Cuphea avigera var. pulcherrima FATB 1) SEQ ID NO: 170 44 WO 2014/151904 PCT/US2014/026644 CpauFATB1 (Cuphea paucipetala FATB1) SEQ ID NO: 171 CprocFATB 1 (Cuphea procumbens FATB 1) SEQ ID NO: 172 CprocFATB2 (Cuphea procumbens FATB2) SEQ ID NO: 173 CprocFATB3 (Cupheaprocumbens FATB3) SEQ ID NO: 174 CigneaFATBI (Cuphea ignea FA TB1) SEQ ID NO: 175 CcalcFATBI (Cuphea calcarata FATB1) SEQ ID NO: 176 ChookFATB4 (Cuphea hookeriana FATB4) SEQ ID NO: 177 CaFATBI (Cuphea avigera var. pulcherrima FATB1) SEQ ID NO: 178 CpauFATB1 (Cuphea paucipetala FATB1) SEQ ID NO: 179 CprocFATB1 (Cuphea procumbens FATB1) SEQ ID NO: 180 CprocFATB2 (Cuphea procumbens FATB2) SEQ ID NO: 181 CprocFATB3 (Cupheaprocumbens FATB3) SEQ ID NO: 182 CigneaFATBI (Cuphea ignea FA TB1) SEQ ID NO: 183 Example 4. FATB consensus sequences: Discovery, cloning and fatty acid profiles [0094] In the course of testing several new putative midchain FatB thioesterases in UTEX1435, S3150 (Strain A above), we identified several 5 thioesterases with increased C10:0 and C16:0 activity above the background midchain levels found in the strain. We reasoned that a consensus sequence could be obtained for an idealized C 10:0 thioesterase and C 16:0 thioesterase from aligning the best performing C10:0 and C16:0 thioesterases. A consensus C10:0 specific thioesterase sequence was generated using the C. palustris FatB 1 (CpFATB 1), C. PSR23 FatB3 10 (CuPSR23FATB3), C. viscosissima FatBI (CvisFATB1), C. glossostoma FatBI (CgFATB 1), and C. carthagenensis FatB2 (CcrFATB2) sequences as inputs resulting in a C10:0 specific consensus sequence termed JcFATB1/SzFATBl. A consensus C 16:0 specific thioesterase sequence was generated using the C. heterophylla FatB3a (ChtFATB3a), C. carthagenensis FatBI (CcrFATB 1), C. viscosissima FatB2 15 (CvisFATB2), C. hookeriana FatB1 (ChFATB1; AAC48990), C. hyssopifolia FatB2 (ChsFATB2), C. calophylla FatB2 (CcalFATB2; ABB71581), C. hookeriana FatB1-1 (ChFATB1-1; AAC72882), C. lanceolata FatBI (ClFATB1; CAC19933), and C. wrightii FatB4a (CwFATB4a) sequences as inputs resulting in a C16:0 specific consensus sequence termed JcFATB2/SzFATB2. The resulting consensus sequences 45 WO 2014/151904 PCT/US2014/026644 were synthesized, cloned into a vector identical to that used to test other FatB thioesterases, and introduced into S3150 as described above. The consensus amino acid sequences are given as SEQ ID NOs. 106 and 107; the nucleic acid sequences were based on these amino acid sequences using codon optimization for Prototheca 5 moriformis. The transformants were selected, cultivated and the oil was extracted and analyzed by FAME-GC-FID. The fatty acid profiles obtained are given in the table below. FA profile of top performer from each transformation (%; primary lipid) Gene SZ C C C C C C C C C1 Nam Plas 8: 10 12 14 16 18 18 18 8:3 Species e mid Strain 0 :0 :0 :0 :0 :0 :1 :2 a Consensu s S3150; Sequence JcFA pSZ3 T617; 1 TBI 187 D1930-18 0 2 0 2 26 3 57 8 1 Consensu s S3150; Sequence JcFA pSZ3 T600; 2 TB2 100 D1872-17 0 0 0 6 54 3 29 6 0 S3150 (parent 0 0 0 2 28 3 58 7 0 strain): 10 Example 5: Clade analysis Various novel FATB thioesterases were clustered according to a neighbor joining algorithm. These were found to form twelve clades as listed in Table la. Putative function was assigned based on expression in Prototheca as described above. [00951 The described embodiments of the invention are intended to be merely 15 exemplary and numerous variations and modifications will be apparent to those skilled in the art. All such variations and modifications are intended to be within the scope of the present invention. 46 WO 2014/151904 PCT/US2014/026644 Sequence Listing SEQ ID NO 1: Cinnamomum camphora (Cc) FATBib variant M25L, M322R, AT367-D368 amino acid sequence 5 MATTSLASAFCSMKAVMLARDGRGLKPRSSDLQLRAGNAQTSLKMINGTKFSYTESLKKLPD WSMLFAVITTIFSAAEKQWTNLEWKPKPNPPQLLDDHFGPHGLVFRRTFAIRSYEVGPDRSTSI VAVMNHLQEAALNHAKSVGILGDGFGTTLEMSKRDLIWVVKRTHVAVERYPAWGDTVEVE CWVGASGNNGRRHDFLVRDCKTGEILTRCTSLSVMMNTRTRRLSKIPEEVRGEIGPAFIDNVA VKDEEIKKPQKLNDSTADYIQGGLTPRWNDLDINQHVNNIKYVDWILETVPDSIFESHHISSFTI 10 EYRRECTRDSVLQSLTTVSGGSSEAGLVCEHLLQLEGGSEVLRAKTEWRPKLSFRGISVIPAES SV* SEQ ID NO 2: Cinnamomum camphora (Cc) FATBib variant M25L, M322R, AT367-D368 coding DNA sequence TTAGCTTCTGCTTTCTGCTCGATGAAAGCTGTAATGTTGGCTCGTGATGGCAGGGGCTTGA 15 AACCCAGGAGCAGTGATTTGCAGCTGAGGGCGGGAAATGCACAAACCTCTTTGAAGATGA TCAATGGGACCAAGTTCAGTTACACAGAGAGCTTGAAAAAGTTGCCTGACTGGAGCATGC TCTTTGCAGTGATCACGACCATCTTTTCGGCTGCTGAGAAGCAGTGGACCAATCTAGAGTG GAAGCCGAAGCCGAATCCACCCCAGTTGCTTGATGACCATTTTGGGCCGCATGGGTTAGTT TTCAGGCGCACCTTTGCCATCAGATCGTATGAGGTGGGACCTGACCGCTCCACATCTATAG 20 TGGCTGTTATGAATCACTTGCAGGAGGCTGCACTTAATCATGCGAAGAGTGTGGGAATTCT AGGAGATGGATTCGGTACGACGCTAGAGATGAGTAAGAGAGATCTGATATGGGTTGTGAA ACGCACGCATGTTGCTGTGGAACGGTACCCTGCTTGGGGTGATACTGTTGAAGTAGAGTG CTGGGTTGGTGCATCGGGAAATAATGGCAGGCGCCATGATTTCCTTGTCCGGGACTGCAA AACAGGCGAAATTCTTACAAGATGTACCAGTCTTTCGGTGATGATGAATACAAGGACAAG 25 GAGGTTGTCCAAAATCCCTGAAGAAGTTAGAGGGGAGATAGGGCCTGCATTCATTGATAA TGTGGCTGTCAAGGACGAGGAAATTAAGAAACCACAGAAGCTCAATGACAGCACTGCAG ATTACATCCAAGGAGGATTGACTCCTCGATGGAATGATTTGGATATCAATCAGCACGTTA ACAACATCAAATACGTTGACTGGATTCTTGAGACTGTCCCAGACTCAATCTTTGAGAGTCA TCATATTTCCAGCTTCACTATTGAATACAGGAGAGAGTGCACGAGGGATAGCGTGCTGCA 30 GTCCCTGACCACTGTCTCCGGTGGCTCGTCGGAAGCTGGGTTAGTGTGCGAGCACTTGCTC CAGCTTGAAGGTGGGTCTGAGGTATTGAGGGCAAAAACAGAGTGGAGGCCTAAGCTTAGT TTCAGAGGGATTAGTGTGATACCCGCAGAATCGAGTGTCTAA SEQ ID NO 3: Cinnamomum camphora (Cc) FATBib variant M25L, M322R, AT367-D368 coding DNA sequence 35 codon optimized for Prototheca moriformis TTAGCTTCTGCTTTCTGCTCGATGAAAGCTGTAATGTTGGCTCGTGATGGCAGGGGCTTGA AACCCAGGAGCAGTGATTTGCAGCTGAGGGCGGGAAATGCACAAACCTCTTTGAAGATGA TCAATGGGACCAAGTTCAGTTACACAGAGAGCTTGAAAAAGTTGCCTGACTGGAGCATGC 40 TCTTTGCAGTGATCACGACCATCTTTTCGGCTGCTGAGAAGCAGTGGACCAATCTAGAGTG GAAGCCGAAGCCGAATCCACCCCAGTTGCTTGATGACCATTTTGGGCCGCATGGGTTAGTT TTCAGGCGCACCTTTGCCATCAGATCGTATGAGGTGGGACCTGACCGCTCCACATCTATAG TGGCTGTTATGAATCACTTGCAGGAGGCTGCACTTAATCATGCGAAGAGTGTGGGAATTCT AGGAGATGGATTCGGTACGACGCTAGAGATGAGTAAGAGAGATCTGATATGGGTTGTGAA 45 ACGCACGCATGTTGCTGTGGAACGGTACCCTGCTTGGGGTGATACTGTTGAAGTAGAGTG CTGGGTTGGTGCATCGGGAAATAATGGCAGGCGCCATGATTTCCTTGTCCGGGACTGCAA AACAGGCGAAATTCTTACAAGATGTACCAGTCTTTCGGTGATGATGAATACAAGGACAAG GAGGTTGTCCAAAATCCCTGAAGAAGTTAGAGGGGAGATAGGGCCTGCATTCATTGATAA TGTGGCTGTCAAGGACGAGGAAATTAAGAAACCACAGAAGCTCAATGACAGCACTGCAG 50 ATTACATCCAAGGAGGATTGACTCCTCGATGGAATGATTTGGATATCAATCAGCACGTTA ACAACATCAAATACGTTGACTGGATTCTTGAGACTGTCCCAGACTCAATCTTTGAGAGTCA TCATATTTCCAGCTTCACTATTGAATACAGGAGAGAGTGCACGAGGGATAGCGTGCTGCA 47 WO 2014/151904 PCT/US2014/026644 GTCCCTGACCACTGTCTCCGGTGGCTCGTCGGAAGCTGGGTTAGTGTGCGAGCACTTGCTC CAGCTTGAAGGTGGGTCTGAGGTATTGAGGGCAAAAACAGAGTGGAGGCCTAAGCTTAGT TTCAGAGGGATTAGTGTGATACCCGCAGAATCGAGTGTCTAA SEQ ID NO:4 5 Cinnamomum camphora (Cc) FATB4 amino acid sequence MVTTSLASAYFSMKAVMLAPDGRGIKPRSSGLQVRAGNERNSCKVINGTKVKDTEGLKGCST LQGQSMLDDHFGLHGLVFRRTFAIRCYEVGPDRSTSIMAVMNHLQEAARNHAESLGLLGDGF GETLEMSKRDLIWVVRRTHVAVERYPAWGDTVEVEAWVGASGNTGMRRDFLVRDCKTGHI LTRCTSVSVMMNMRTRRLSKIPQEVRAEIDPLFIEKVAVKEGEIKKLQKLNDSTADYIQGGWT 10 PRWNDLDVNQHVNNIIYVGWIFKSVPDSISENHHLSSITLEYRRECTRGNKLQSLTTVCGGSSE AGIICEHLLQLEDGSEVLRARTEWRPKHTDSFQGISERFPQQEPHK SEQ ID NO: 5 Cinnamomum camphora (Cc) FATB4 coding DNA sequence ATGGTCACCACCTCTTTAGCTTCCGCTTACTTCTCGATGAAAGCTGTAATGTTGGCTCCTGA 15 CGGCAGGGGCATAAAGCCCAGGAGCAGTGGTTTGCAGGTGAGGGCGGGAAATGAACGAA ACTCTTGCAAGGTGATCAATGGGACCAAGGTCAAAGACACGGAGGGCTTGAAAGGGTGC AGCACGTTGCAAGGCCAGAGCATGCTTGATGACCATTTTGGTCTGCATGGGCTAGTTTTCA GGCGCACCTTTGCAATCAGATGCTATGAGGTTGGACCTGACCGCTCCACATCCATAATGGC TGTTATGAATCACTTGCAGGAAGCTGCACGTAATCATGCGGAGAGTCTGGGACTTCTAGG 20 AGATGGATTCGGTGAGACACTGGAGATGAGTAAGAGAGATCTGATATGGGTTGTGAGACG CACGCATGTTGCTGTGGAACGGTACCCTGCTTGGGGCGATACTGTTGAAGTCGAGGCCTG GGTGGGTGCATCAGGTAACACTGGCATGCGCCGCGATTTCCTTGTCCGCGACTGCAAAAC TGGCCACATTCTTACAAGATGTACCAGTGTTTCAGTGATGATGAATATGAGGACAAGGAG ATTGTCCAAAATTCCCCAAGAAGTTAGAGCGGAGATTGACCCTCTTTTCATTGAAAAGGTT 25 GCTGTCAAGGAAGGGGAAATTAAAAAATTACAGAAGTTGAATGATAGCACTGCAGATTAC ATTCAAGGGGGTTGGACTCCTCGATGGAATGATTTGGATGTCAATCAGCACGTGAACAAT ATCATATACGTTGGCTGGATTTTTAAGAGCGTCCCAGACTCTATCTCTGAGAATCATCATC TTTCTAGCATCACTCTCGAATACAGGAGAGAGTGCACAAGGGGCAACAAGCTGCAGTCCC TGACCACTGTTTGTGGTGGCTCGTCGGAAGCTGGGATCATATGTGAGCACCTACTCCAGCT 30 TGAGGATGGGTCTGAGGTTTTGAGGGCAAGAACAGAGTGGAGGCCCAAGCACACCGATA GTTTCCAAGGCATTAGTGAGAGATTCCCGCAGCAAGAACCGCATAAGTAA SEQ ID NO: 6 Cinnamomum camphora (Cc) FATB4 coding DNA sequence codon optimized for Prototheca moriformis 35 ATGGTGACCACCTCCCTGGCCTCCGCCTACTTCTCCATGAAGGCCGTGATGCTGGCCCCCG ACGGCCGCGGCATCAAGCCCCGCTCCTCCGGCCTGCAGGTGCGCGCCGGCAACGAGCGCA ACTCCTGCAAGGTGATCAACGGCACCAAGGTGAAGGACACCGAGGGCCTGAAGGGCTGC TCCACCCTGCAGGGCCAGTCCATGCTGGACGACCACTTCGGCCTGCACGGCCTGGTGTTCC GCCGCACCTTCGCCATCCGCTGCTACGAGGTGGGCCCCGACCGCTCCACCTCCATCATGGC 40 CGTGATGAACCACCTGCAGGAGGCCGCCCGCAACCACGCCGAGTCCCTGGGCCTGCTGGG CGACGGCTTCGGCGAGACCCTGGAGATGTCCAAGCGCGACCTGATCTGGGTGGTGCGCCG CACCCACGTGGCCGTGGAGCGCTACCCCGCCTGGGGCGACACCGTGGAGGTGGAGGCCTG GGTGGGCGCCTCCGGCAACACCGGCATGCGCCGCGACTTCCTGGTGCGCGACTGCAAGAC CGGCCACATCCTGACCCGCTGCACCTCCGTGTCCGTGATGATGAACATGCGCACCCGCCGC 45 CTGTCCAAGATCCCCCAGGAGGTGCGCGCCGAGATCGACCCCCTGTTCATCGAGAAGGTG GCCGTGAAGGAGGGCGAGATCAAGAAGCTGCAGAAGCTGAACGACTCCACCGCCGACTA CATCCAGGGCGGCTGGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAA CATCATCTACGTGGGCTGGATCTTCAAGTCCGTGCCCGACTCCATCTCCGAGAACCACCAC CTGTCCTCCATCACCCTGGAGTACCGCCGCGAGTGCACCCGCGGCAACAAGCTGCAGTCC 50 CTGACCACCGTGTGCGGCGGCTCCTCCGAGGCCGGCATCATCTGCGAGCACCTGCTGCAG CTGGAGGACGGCTCCGAGGTGCTGCGCGCCCGCACCGAGTGGCGCCCCAAGCACACCGAC TCCTTCCAGGGCATCTCCGAGCGCTTCCCCCAGCAGGAGCCCCACAAGTGA 48 WO 2014/151904 PCT/US2014/026644 SEQ ID NO: 7 Cinnamomum camphora (Cc) FATB3 amino acid sequence MVATAAASAFFPVGAPATSSATSAKASMMPDNLDARGIKPKPASSSGLQVKANAHASPKING SKVSTDTLKGEDTLTSSPAPRTFINQLPDWSMFLAAITTIFLAAEKQWTNLDWKPRRPDMLAD 5 PFGIGRFMQDGLIFRQHFAIRSYEIGADRTASIETLMNHLQETALNHVRSAGLLGDGFGATPEM SRRDLIWVVTRMQVLVDRYPAWGDIVEVETWVGASGKNGMRRDWLVRDSQTGEILTRATSV WVMMNKRTRRLSKLPEEVRGEIGPYFIEDVAIIEEDNRKLQKLNENTADNVRRGLTPRWSDLD VNQHVNNVKYIGWILESAPGSILESHELSCMTLEYRRECGKDSVLQSMTAVSGGGSAAGGSPE SSVECDHLLQLESGPEVVRGRTEWRPKSANNSRSILEMPAESL 10 SEQ ID NO: 8 Cinnamomum camphora (Cc) FATB3 coding DNA sequence ATGGTTGCCACCGCTGCTGCTTCTGCTTTCTTCCCGGTCGGTGCTCCGGCTACGTCATCTGC AACTTCAGCCAAAGCGTCGATGATGCCTGATAATTTGGATGCCAGAGGCATCAAACCGAA GCCGGCTTCGTCCAGCGGCTTGCAGGTTAAGGCAAATGCCCATGCCTCTCCCAAGATTAAT 15 GGTTCCAAGGTGAGCACGGATACCTTGAAGGGGGAAGACACCTTAACTTCCTCGCCCGCC CCACGGACCTTTATCAACCAATTGCCTGACTGGAGCATGTTCCTTGCTGCCATCACAACTA TTTTCTTGGCTGCCGAGAAGCAGTGGACGAATCTCGACTGGAAGCCCAGAAGACCCGACA TGCTTGCTGACCCGTTTGGCATCGGGAGGTTTATGCAGGATGGGCTGATTTTCAGGCAGCA CTTTGCAATCAGATCTTATGAGATTGGGGCTGATAGAACGGCGTCTATAGAGACTTTAATG 20 AATCACTTGCAGGAGACTGCACTTAATCATGTGAGGAGTGCTGGACTCCTAGGTGATGGA TTTGGTGCGACACCTGAGATGAGTAGAAGAGATCTGATATGGGTTGTAACACGTATGCAG GTTCTTGTGGACCGCTACCCTGCTTGGGGTGATATTGTTGAAGTAGAGACCTGGGTTGGTG CATCTGGAAAAAATGGTATGCGCCGTGATTGGCTTGTTCGGGACAGCCAAACTGGTGAAA TTCTCACACGAGCTACCAGTGTTTGGGTGATGATGAATAAACGGACAAGGCGATTGTCCA 25 AACTTCCTGAAGAAGTTAGAGGGGAAATAGGGCCTTATTTTATAGAAGATGTTGCTATCA TAGAGGAGGACAACAGGAAACTACAGAAGCTCAATGAAAACACTGCTGATAATGTTCGA AGGGGTTTGACTCCTCGCTGGAGTGATCTGGATGTTAATCAGCATGTGAACAATGTCAAAT ACATTGGTTGGATTCTTGAGAGTGCACCAGGATCCATCTTGGAGAGTCATGAGCTTTCCTG CATGACCCTTGAATACAGGAGAGAATGTGGGAAGGACAGTGTGCTGCAGTCAATGACTGC 30 TGTCTCTGGTGGAGGCAGTGCAGCAGGTGGCTCACCAGAATCTAGCGTTGAGTGTGACCA CTTGCTCCAGCTAGAGAGTGGGCCTGAAGTTGTGAGGGGAAGAACCGAGTGGAGGCCCA AGAGTGCTAATAACTCGAGGAGCATCCTGGAGATGCCGGCCGAGAGC SEQ ID NO: 9 Cinnamomum camphora (Cc) FATB4 coding DNA sequence codon optimized for Prototheca 35 moriformis ATGGTGGCCACCGCCGCCGCCTCCGCCTTCTTCCCCGTGGGCGCCCCCGCCACCTCCTCCG CCACCTCCGCCAAGGCCTCCATGATGCCCGACAACCTGGACGCCCGCGGCATCAAGCCCA AGCCCGCCTCCTCCTCCGGCCTGCAGGTGAAGGCCAACGCCCACGCCTCCCCCAAGATCA ACGGCTCCAAGGTGTCCACCGACACCCTGAAGGGCGAGGACACCCTGACCTCCTCCCCCG 40 CCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGTTCCTGGCCGCCATCACCAC CATCTTCCTGGCCGCCGAGAAGCAGTGGACCAACCTGGACTGGAAGCCCCGCCGCCCCGA CATGCTGGCCGACCCCTTCGGCATCGGCCGCTTCATGCAGGACGGCCTGATCTTCCGCCAG CACTTCGCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGA TGAACCACCTGCAGGAGACCGCCCTGAACCACGTGCGCTCCGCCGGCCTGCTGGGCGACG 45 GCTTCGGCGCCACCCCCGAGATGTCCCGCCGCGACCTGATCTGGGTGGTGACCCGCATGC AGGTGCTGGTGGACCGCTACCCCGCCTGGGGCGACATCGTGGAGGTGGAGACCTGGGTGG GCGCCTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGGTGCGCGACTCCCAGACCGGCG AGATCCTGACCCGCGCCACCTCCGTGTGGGTGATGATGAACAAGCGCACCCGCCGCCTGT CCAAGCTGCCCGAGGAGGTGCGCGGCGAGATCGGCCCCTACTTCATCGAGGACGTGGCCA 50 TCATCGAGGAGGACAACCGCAAGCTGCAGAAGCTGAACGAGAACACCGCCGACAACGTG CGCCGCGGCCTGACCCCCCGCTGGTCCGACCTGGACGTGAACCAGCACGTGAACAACGTG AAGTACATCGGCTGGATCCTGGAGTCCGCCCCCGGCTCCATCCTGGAGTCCCACGAGCTGT CCTGCATGACCCTGGAGTACCGCCGCGAGTGCGGCAAGGACTCCGTGCTGCAGTCCATGA 49 WO 2014/151904 PCT/US2014/026644 CCGCCGTGTCCGGCGGCGGCTCCGCCGCCGGCGGCTCCCCCGAGTCCTCCGTGGAGTGCG ACCACCTGCTGCAGCTGGAGTCCGGCCCCGAGGTGGTGCGCGGCCGCACCGAGTGGCGCC CCAAGTCCGCCAACAACTCCCGCTCCATCCTGGAGATGCCCGCCGAGTCCCTGTGA SEQ ID NO: 10 5 Cuphea hyssopifolia (Chs) FATB1 amino acid sequence MVATNAAAFSAYTFFLTSPTHGYSSKRLADTQNGYPGTSLKSKSTPPPAAAAARNGALPLLAS ICKCPKKADGSMQLDSSLVFGFQFYIRSYEVGADQTVSIQTVLNYLQEAAINHVQSAGYFGDS FGATPEMTKRNLIWVITKMQVLVDRYPAWGDVVQVDTWTCSSGKNSMQRDWFVRDLKTGD IITRASSVWVLMNRLTRKLSKIPEAVLEEAKLFVMNTAPTVDDNRKLPKLDGSSADYVLSGLT 10 PRWSDLDMNQHVNNVKYIAWILESVPQSIPETHKLSAITVEYRRECGKNSVLQSLTNVSGDGI TCGNSIIECHHLLQLETGPEILLARTEWISKEPGFRGAPIQAEKVYNNK* SEQ ID NO: 11 Cuphea hyssopifolia (Chs) FATB1 coding DNA sequence ATGGTTGCCACTAATGCTGCTGCCTTTTCTGCTTATACTTTCTTCCTTACTTCACCAACTCA 15 TGGTTACTCTTCCAAACGTCTCGCCGATACTCAAAATGGTTATCCGGGTACCTCCTTGAAA TCGAAATCCACTCCTCCACCAGCTGCTGCTGCTGCTCGTAACGGTGCATTGCCACTGCTGG CCTCCATCTGCAAATGCCCCAAAAAGGCTGATGGGAGTATGCAACTAGACAGCTCCTTGG TCTTCGGGTTTCAATTTTACATTAGATCATATGAAGTGGGTGCGGATCAAACCGTGTCAAT ACAGACAGTACTCAATTACTTACAGGAGGCAGCCATCAATCATGTTCAGAGTGCTGGCTA 20 TTTTGGTGATAGTTTTGGCGCCACCCCGGAAATGACCAAGAGGAACCTCATCTGGGTTATC ACTAAGATGCAGGTTTTGGTGGATCGCTATCCCGCTTGGGGCGATGTTGTTCAAGTTGATA CATGGACCTGTAGTTCTGGTAAAAACAGCATGCAGCGTGATTGGTTCGTACGGGATCTCA AAACTGGAGATATTATAACAAGAGCCTCGAGCGTGTGGGTGCTGATGAATAGACTCACCA GAAAATTATCAAAAATTCCTGAAGCAGTTCTGGAAGAAGCAAAACTTTTTGTGATGAACA 25 CTGCCCCCACCGTAGATGACAACAGGAAGCTACCAAAGCTGGATGGCAGCAGTGCTGATT ATGTCCTCTCTGGCTTAACTCCTAGATGGAGCGACTTAGATATGAACCAGCATGTCAACAA TGTGAAGTACATAGCCTGGATCCTTGAGAGTGTCCCTCAGAGCATACCGGAGACACACAA GCTGTCAGCGATAACCGTGGAGTACAGGAGAGAATGTGGCAAGAACAGCGTCCTCCAGTC TCTGACCAACGTCTCCGGGGATGGAATCACATGTGGAAACAGTATTATCGAGTGCCACCA 30 TTTGCTTCAACTTGAGACTGGCCCAGAGATTCTACTAGCGCGGACGGAGTGGATATCCAA GGAACCTGGGTTCAGGGGAGCTCCAATCCAGGCAGAGAAAGTCTACAACAACAAATAA SEQ ID NO: 12 Cuphea hyssopifolia (Chs) FATB1 coding DNA sequence codon optimized for Prototheca morzformis ATGGTGGCCACCAACGCCGCCGCCTTCTCCGCCTACACCTTCTTCCTGACCTCCCCCACCC 35 ACGGCTACTCCTCCAAGCGCCTGGCCGACACCCAGAACGGCTACCCCGGCACCTCCCTGA AGTCCAAGTCCACCCCCCCCCCCGCCGCCGCCGCCGCCCGCAACGGCGCCCTGCCCCTGCT GGCCTCCATCTGCAAGTGCCCCAAGAAGGCCGACGGCTCCATGCAGCTGGACTCCTCCCT GGTGTTCGGCTTCCAGTTCTACATCCGCTCCTACGAGGTGGGCGCCGACCAGACCGTGTCC ATCCAGACCGTGCTGAACTACCTGCAGGAGGCCGCCATCAACCACGTGCAGTCCGCCGGC 40 TACTTCGGCGACTCCTTCGGCGCCACCCCCGAGATGACCAAGCGCAACCTGATCTGGGTG ATCACCAAGATGCAGGTGCTGGTGGACCGCTACCCCGCCTGGGGCGACGTGGTGCAGGTG GACACCTGGACCTGCTCCTCCGGCAAGAACTCCATGCAGCGCGACTGGTTCGTGCGCGAC CTGAAGACCGGCGACATCATCACCCGCGCCTCCTCCGTGTGGGTGCTGATGAACCGCCTG ACCCGCAAGCTGTCCAAGATCCCCGAGGCCGTGCTGGAGGAGGCCAAGCTGTTCGTGATG 45 AACACCGCCCCCACCGTGGACGACAACCGCAAGCTGCCCAAGCTGGACGGCTCCTCCGCC GACTACGTGCTGTCCGGCCTGACCCCCCGCTGGTCCGACCTGGACATGAACCAGCACGTG AACAACGTGAAGTACATCGCCTGGATCCTGGAGTCCGTGCCCCAGTCCATCCCCGAGACC CACAAGCTGTCCGCCATCACCGTGGAGTACCGCCGCGAGTGCGGCAAGAACTCCGTGCTG CAGTCCCTGACCAACGTGTCCGGCGACGGCATCACCTGCGGCAACTCCATCATCGAGTGC 50 CACCACCTGCTGCAGCTGGAGACCGGCCCCGAGATCCTGCTGGCCCGCACCGAGTGGATC TCCAAGGAGCCCGGCTTCCGCGGCGCCCCCATCCAGGCCGAGAAGGTGTACAACAACAAG TGA 50 WO 2014/151904 PCT/US2014/026644 SEQ ID NO: 13 Cuphea hyssopifolia (Chs) FATB2 amino acid sequence MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAPPKINGSSVG LKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDP 5 FGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFGRTLEM YKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASS VWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTPKWNDL DVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKGSGSQFQ HLLRLEDGGEIVKGRTEWRPKTAGINGPIASGETSPGDSS* 10 SEQ ID NO: 14 Cuphea hyssopifolia (Chs) FATB2 coding DNA sequence ATGGTGGCTACCGCTGCAAGTTCAGCATTCTTCCCTGTGCCGTCCCCCGACGCCTCCTCTA GACCTGGAAAGCTCGGCAATGGGTCATCGAGCTTGAGCCCCCTCAAGCCCAAATTGATGG CCAATGGCGGGTTGCAGGTTAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTCTT 15 CGGTCGGTCTAAAGTCCGGCAGTCTCAAGACTCAGGAAGACACTCCTTCGGCGCCTCCTCC CCGGACTTTTATTAACCAGCTGCCTGATTGGAGTATGCTTCTTGCTGCAATCACTACTGTCT TCTTGGCAGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCCAAGAGGCCTGACATGC TTGTGGACCCGTTCGGATTGGGAAGGATTGTTCAAGATGGGCTTGTGTTCAGGCAGAATTT TTCGATTAGGTCCTATGAAATAGGCGCTGATCGCACTGCGTCTATAGAGACGGTGATGAA 20 CCACTTGCAGGAAACAGCTCTCAATCATGTTAAGAGTGCTGGGCTTCTTAATGACGGCTTT GGTCGTACTCTTGAGATGTATAAAAGGGACCTTATTTGGGTTGTTGCAAAAATGCAGGTCA TGGTTAACCGCTATCCTACTTGGGGCGACACGGTTGAAGTGAATACTTGGGTTGCCAAGTC AGGGAAAAATGGTATGCGTCGTGATTGGCTCATAAGTGATTGCAATACAGGAGAAATTCT TACTAGAGCATCAAGTGTGTGGGTCATGATGAATCAAAAGACAAGAAGATTGTCAAAAAT 25 TCCAGATGAGGTTCGACATGAGATAGAGCCTCATTTCGTGGACTCTGCTCCCGTCATTGAA GATGATGACCGGAAACTTCCCAAGCTGGATGAGAAGACTGCTGACTCCATCCGCAAGGGT CTAACTCCGAAGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTACATT GGGTGGATTCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGTTCCCTTA CCCTGGAATATAGGCGGGAATGCGGAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTGTGG 30 ACCCCTCTGGAAAGGGCTCTGGGTCTCAGTTCCAGCACCTTCTGCGGCTTGAGGATGGAG GTGAGATTGTGAAGGGGAGAACTGAGTGGCGACCCAAGACTGCAGGAATCAATGGGCCA ATAGCATCCGGGGAGACCTCACCTGGAGACTCTTCTTAG SEQ ID NO: 15 Cuphea hyssopifolia (Chs) FATB2 coding DNA sequence codon optimized for Prototheca morzformis 35 ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCCCCGACGCCTCCTCCCG CCCCGGCAAGCTGGGCAACGGCTCCTCCTCCCTGTCCCCCCTGAAGCCCAAGCTGATGGCC AACGGCGGCCTGCAGGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCC GTGGGCCTGAAGTCCGGCTCCCTGAAGACCCAGGAGGACACCCCCTCCGCCCCCCCCCCC CGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGGCCGCCATCACCACCGTGT 40 TCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAAGCGCCCCGACATGC TGGTGGACCCCTTCGGCCTGGGCCGCATCGTGCAGGACGGCCTGGTGTTCCGCCAGAACTT CTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAA CCACCTGCAGGAGACCGCCCTGAACCACGTGAAGTCCGCCGGCCTGCTGAACGACGGCTT CGGCCGCACCCTGGAGATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGT 45 GATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCCAA GTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGAT CCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGACCCGCCGCCTGTCCAA GATCCCCGACGAGGTGCGCCACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCCGTGAT CGAGGACGACGACCGCAAGCTGCCCAAGCTGGACGAGAAGACCGCCGACTCCATCCGCA 50 AGGGCCTGACCCCCAAGTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAG TACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGC TCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACC GCCGTGGACCCCTCCGGCAAGGGCTCCGGCTCCCAGTTCCAGCACCTGCTGCGCCTGGAG 51 WO 2014/151904 PCT/US2014/026644 GACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGACCGCCGGCATCAA CGGCCCCATCGCCTCCGGCGAGACCTCCCCCGGCGACTCCTCCTGA SEQ ID NO: 16 Cuphea hyssopifolia (Chs) FATB2b +a.a.248-259 variant amino acid sequence 5 MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAPPKINGSSVG LKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDP FGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFGRTLEM YKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASS KSQIMLPLHYCSVWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADS 10 IRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTA VDPSGKGSGSQFQHLLRLEDGGEIVKGRTEWRPKTAGINGPIASGETSPGDSS* SEQ ID NO: 17 Cuphea hyssopifolia (Chs) FATB2b+a.a.248-259 variant coding DNA sequence ATGGTGGCTACCGCTGCAAGTTCAGCATTCTTCCCTGTGCCGTCCCCCGACGCCTCCTCTA 15 GACCTGGAAAGCTCGGCAATGGGTCATCGAGCTTGAGCCCCCTCAAGCCCAAATTGATGG CCAATGGCGGGTTGCAGGTTAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTCTT CGGTCGGTCTAAAGTCCGGCAGTCTCAAGACTCAGGAAGACACTCCTTCGGCGCCTCCTCC CCGGACTTTTATTAACCAGCTGCCTGATTGGAGTATGCTTCTTGCTGCAATCACTACTGTCT TCTTGGCAGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCCAAGAGGCCTGACATGC 20 TTGTGGACCCGTTCGGATTGGGAAGGATTGTTCAAGATGGGCTTGTGTTCAGGCAGAATTT TTCGATTAGGTCCTATGAAATAGGCGCTGATCGCACTGCGTCTATAGAGACGGTGATGAA CCACTTGCAGGAAACAGCTCTCAATCATGTTAAGAGTGCTGGGCTTCTTAATGACGGCTTT GGTCGTACTCTTGAGATGTATAAAAGGGACCTTATTTGGGTTGTTGCAAAAATGCAGGTCA TGGTTAACCGCTATCCTACTTGGGGCGACACGGTTGAAGTGAATACTTGGGTTGCCAAGTC 25 AGGGAAAAATGGTATGCGTCGTGATTGGCTCATAAGTGATTGCAATACAGGAGAAATTCT TACTAGAGCATCAAGTAAAAGCCAAATTATGTTACCCTTACATTATTGCAGTGTGTGGGTC ATGATGAATCAAAAGACAAGAAGATTGTCAAAAATTCCAGATGAGGTTCGACATGAGATA GAGCCTCATTTCGTGGACTCTGCTCCCGTCATTGAAGATGATGACCGGAAACTTCCCAAGC TGGATGAGAAGACTGCTGACTCCATCCGCAAGGGTCTAACTCCGAAGTGGAATGACTTGG 30 ATGTCAATCAGCACGTCAACAACGTGAAGTACATTGGGTGGATTCTTGAGAGTACTCCAC CAGAAGTTCTGGAGACCCAGGAGTTATGTTCCCTTACCCTGGAATATAGGCGGGAATGCG GAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTGTGGACCCCTCTGGAAAGGGCTCTGGGT CTCAGTTCCAGCACCTTCTGCGGCTTGAGGATGGAGGTGAGATTGTGAAGGGGAGAACTG AGTGGCGACCCAAGACTGCAGGAATCAATGGGCCAATAGCATCCGGGGAGACCTCACCTG 35 GAGACTCTTCTTAG SEQ ID NO: 18 Cuphea hyssopifolia (Chs) FATB2b +a.a.248-259 variant coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCCCCGACGCCTCCTCCCG 40 CCCCGGCAAGCTGGGCAACGGCTCCTCCTCCCTGTCCCCCCTGAAGCCCAAGCTGATGGCC AACGGCGGCCTGCAGGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCC GTGGGCCTGAAGTCCGGCTCCCTGAAGACCCAGGAGGACACCCCCTCCGCCCCCCCCCCC CGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGGCCGCCATCACCACCGTGT TCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAAGCGCCCCGACATGC 45 TGGTGGACCCCTTCGGCCTGGGCCGCATCGTGCAGGACGGCCTGGTGTTCCGCCAGAACTT CTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAA CCACCTGCAGGAGACCGCCCTGAACCACGTGAAGTCCGCCGGCCTGCTGAACGACGGCTT CGGCCGCACCCTGGAGATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGT GATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCCAA 50 GTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGAT CCTGACCCGCGCCTCCTCCAAGTCCCAGATCATGCTGCCCCTGCACTACTGCTCCGTGTGG GTGATGATGAACCAGAAGACCCGCCGCCTGTCCAAGATCCCCGACGAGGTGCGCCACGAG 52 WO 2014/151904 PCT/US2014/026644 ATCGAGCCCCACTTCGTGGACTCCGCCCCCGTGATCGAGGACGACGACCGCAAGCTGCCC AAGCTGGACGAGAAGACCGCCGACTCCATCCGCAAGGGCCTGACCCCCAAGTGGAACGA CCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGATCCTGGAGTCCAC CCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGCCGCGA 5 GTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCGCCGTGGACCCCTCCGGCAAGGGCTC CGGCTCCCAGTTCCAGCACCTGCTGCGCCTGGAGGACGGCGGCGAGATCGTGAAGGGCCG CACCGAGTGGCGCCCCAAGACCGCCGGCATCAACGGCCCCATCGCCTCCGGCGAGACCTC CCCCGGCGACTCCTCCTGA SEQ ID NO: 19 10 Cuphea hyssopifolia (Chs) FATB3 amino acid sequence MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASARPKANGSA VSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLM DPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEM CKRDLIWVVTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDCHTGEILIRATSMC 15 AMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRWNDLDV NQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRRECGRDSVLESVTAMDPSKEGDRSLYQH LLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS* SEQ ID NO: 20 Cuphea hyssopifolia (Chs) FATB3 coding DNA sequence 20 ATGGTGGCTGCCGAAGCAAGTTCTGCACTCTTCTCCGTTCGAACCCCGGGAACCTCCCCTA AACCCGGGAAGTTCGGGAATTGGCCAACGAGCTTGAGCGTCCCCTTCAAGTCCAAATCAA ACCACAATGGCGGCTTTCAGGTTAAGGCAAACGCCAGTGCCCGTCCTAAGGCTAACGGTT CTGCAGTAAGTCTAAAGTCTGGCAGCCTCGACACTCAGGAGGACACTTCATCGTCGTCCTC TCCTCCTCGGACTTTCATTAACCAGTTGCCCGACTGGAGTATGCTGCTGTCCGCGATCACG 25 ACCGTCTTCGTGGCGGCTGAGAAGCAGTGGACGATGCTTGATCGGAAATCTAAGAGGCCC GACATGCTCATGGACCCGTTTGGGGTTGACAGGGTTGTTCAGGATGGGGCTGTGTTCAGA CAGAGTTTTTCGATTAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAGACG CTGATGAACATCTTCCAGGAAACATCTCTCAATCATTGTAAGAGTATCGGTCTTCTCAATG ACGGCTTTGGTCGTACTCCTGAGATGTGTAAGAGGGACCTCATTTGGGTGGTTACAAAAAT 30 GCACGTCGAGGTTAATCGCTATCCTACTTGGGGTGATACTATCGAGGTCAATACTTGGGTC TCCGAGTCGGGGAAAACCGGTATGGGTCGTGATTGGCTGATAAGTGATTGTCATACAGGA GAAATTCTAATAAGAGCAACGAGCATGTGTGCTATGATGAATCAAAAGACGAGAAGATTC TCAAAATTTCCATATGAGGTTCGACAGGAGTTGGCGCCTCATTTTGTGGACTCTGCTCCTG TCATTGAAGACTATCAAAAATTGCACAAGCTTGATGTGAAGACGGGTGATTCCATTTGCA 35 ATGGCCTAACTCCAAGGTGGAATGACTTGGATGTCAATCAGCACGTTAACAATGTGAAGT ACATTGGGTGGATTCTCGAGAGTGTTCCAACGGAAGTTTTCGAGACCCAGGAGCTATGTG GCCTCACCCTTGAGTATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCCGTGACCG CTATGGATCCATCAAAAGAGGGAGACAGATCTCTGTACCAGCACCTTCTTCGGCTTGAGG ATGGGGCTGATATCGCGAAGGGCAGAACCAAGTGGCGGCCGAAGAATGCAGGAACCAAT 40 GGGGCAATATCAACAGGAAAGACTTCAAATGGAAACTCGATCTCTTAG SEQ ID NO: 21 Cuphea hyssopifolia (Chs) FATB3 coding DNA sequence codon optimized for Prototheca morzformis ATGGTGGCCGCCGAGGCCTCCTCCGCCCTGTTCTCCGTGCGCACCCCCGGCACCTCCCCCA AGCCCGGCAAGTTCGGCAACTGGCCCACCTCCCTGTCCGTGCCCTTCAAGTCCAAGTCCAA 45 CCACAACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCGCCCCAAGGCCAACGGCTC CGCCGTGTCCCTGAAGTCCGGCTCCCTGGACACCCAGGAGGACACCTCCTCCTCCTCCTCC CCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGTCCGCCATCACCA CCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCG ACATGCTGATGGACCCCTTCGGCGTGGACCGCGTGGTGCAGGACGGCGCCGTGTTCCGCC 50 AGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCT GATGAACATCTTCCAGGAGACCTCCCTGAACCACTGCAAGTCCATCGGCCTGCTGAACGA CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGAT 53 WO 2014/151904 PCT/US2014/026644 GCACGTGGAGGTGAACCGCTACCCCACCTGGGGCGACACCATCGAGGTGAACACCTGGGT GTCCGAGTCCGGCAAGACCGGCATGGGCCGCGACTGGCTGATCTCCGACTGCCACACCGG CGAGATCCTGATCCGCGCCACCTCCATGTGCGCCATGATGAACCAGAAGACCCGCCGCTT CTCCAAGTTCCCCTACGAGGTGCGCCAGGAGCTGGCCCCCCACTTCGTGGACTCCGCCCCC 5 GTGATCGAGGACTACCAGAAGCTGCACAAGCTGGACGTGAAGACCGGCGACTCCATCTGC AACGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAA GTACATCGGCTGGATCCTGGAGTCCGTGCCCACCGAGGTGTTCGAGACCCAGGAGCTGTG CGGCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGAC CGCCATGGACCCCTCCAAGGAGGGCGACCGCTCCCTGTACCAGCACCTGCTGCGCCTGGA 10 GGACGGCGCCGACATCGCCAAGGGCCGCACCAAGTGGCGCCCCAAGAACGCCGGCACCA ACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCATCTCCTGA SEQ ID NO: 22 Cuphea hyssopifolia (Chs) FATB3b (V2041,C239F, E243D, M25 1V variant) amino acid sequence MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASARPKANGSA 15 VSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLM DPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEM CKRDLIWVVTKMHIEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDFHTGDILIRATSVC AMMNQKTRRF SKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRWNDLDV NQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRRECGRDSVLESVTAMDPSKEGDRSLYQH 20 LLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS* SEQ ID NO: 23 Cuphea hyssopifolia (Chs) FATB3b (V2041,C239F, E243D, M25 1V variant) coding DNA sequence ATGGTGGCTGCCGAAGCAAGTTCTGCACTCTTCTCCGTTCGAACCCCGGGAACCTCCCCTA AACCCGGGAAGTTCGGGAATTGGCCAACGAGCTTGAGCGTCCCCTTCAAGTCCAAATCAA 25 ACCACAATGGCGGCTTTCAGGTTAAGGCAAACGCCAGTGCCCGTCCTAAGGCTAACGGTT CTGCAGTAAGTCTAAAGTCTGGCAGCCTCGACACTCAGGAGGACACTTCATCGTCGTCCTC TCCTCCTCGGACTTTCATTAACCAGTTGCCCGACTGGAGTATGCTGCTGTCCGCGATCACG ACCGTCTTCGTGGCGGCTGAGAAGCAGTGGACGATGCTTGATCGGAAATCTAAGAGGCCC GACATGCTCATGGACCCGTTTGGGGTTGACAGGGTTGTTCAGGATGGGGCTGTGTTCAGA 30 CAGAGTTTTTCGATTAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAGACG CTGATGAACATCTTCCAGGAAACATCTCTCAATCATTGTAAGAGTATCGGTCTTCTCAATG ACGGCTTTGGTCGTACTCCTGAGATGTGTAAGAGGGACCTCATTTGGGTGGTTACAAAAAT GCACATCGAGGTTAATCGCTATCCTACTTGGGGTGATACTATCGAGGTCAATACTTGGGTC TCCGAGTCGGGGAAAACCGGTATGGGTCGTGATTGGCTGATAAGTGATTTTCATACAGGA 35 GACATTCTAATAAGAGCAACGAGCGTGTGTGCTATGATGAATCAAAAGACGAGAAGATTC TCAAAATTTCCATATGAGGTTCGACAGGAGTTAGCGCCTCATTTTGTGGACTCTGCTCCAG TCATTGAAGACTATCAAAAATTGCACAAGCTTGATGTGAAGACGGGTGATTCCATTTGCA ATGGCCTAACTCCAAGGTGGAATGACTTGGATGTCAATCAGCACGTTAACAATGTGAAGT ACATTGGGTGGATTCTCGAGAGTGTTCCAACGGAAGTTTTCGAGACCCAGGAGCTATGTG 40 GCCTCACCCTTGAGTATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCCGTGACCG CTATGGATCCCTCAAAAGAGGGAGACAGATCTCTGTACCAGCACCTTCTTCGGCTTGAGG ATGGGGCTGATATCGCGAAGGGCAGAACCAAGTGGCGGCCGAAGAATGCAGGAACCAAT GGGGCAATATCAACAGGAAAGACTTCAAATGGAAACTCGATCTCTTAG SEQ ID NO: 24 45 Cuphea hyssopifolia (Chs) FATB3b (V204I,C239F, E243D, M25 1V variant) coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCGCCGAGGCCTCCTCCGCCCTGTTCTCCGTGCGCACCCCCGGCACCTCCCCCA AGCCCGGCAAGTTCGGCAACTGGCCCACCTCCCTGTCCGTGCCCTTCAAGTCCAAGTCCAA CCACAACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCGCCCCAAGGCCAACGGCTC 50 CGCCGTGTCCCTGAAGTCCGGCTCCCTGGACACCCAGGAGGACACCTCCTCCTCCTCCTCC CCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGTCCGCCATCACCA CCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCG 54 WO 2014/151904 PCT/US2014/026644 ACATGCTGATGGACCCCTTCGGCGTGGACCGCGTGGTGCAGGACGGCGCCGTGTTCCGCC AGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCT GATGAACATCTTCCAGGAGACCTCCCTGAACCACTGCAAGTCCATCGGCCTGCTGAACGA CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGAT 5 GCACATCGAGGTGAACCGCTACCCCACCTGGGGCGACACCATCGAGGTGAACACCTGGGT GTCCGAGTCCGGCAAGACCGGCATGGGCCGCGACTGGCTGATCTCCGACTTCCACACCGG CGACATCCTGATCCGCGCCACCTCCGTGTGCGCCATGATGAACCAGAAGACCCGCCGCTT CTCCAAGTTCCCCTACGAGGTGCGCCAGGAGCTGGCCCCCCACTTCGTGGACTCCGCCCCC GTGATCGAGGACTACCAGAAGCTGCACAAGCTGGACGTGAAGACCGGCGACTCCATCTGC 10 AACGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAA GTACATCGGCTGGATCCTGGAGTCCGTGCCCACCGAGGTGTTCGAGACCCAGGAGCTGTG CGGCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGAC CGCCATGGACCCCTCCAAGGAGGGCGACCGCTCCCTGTACCAGCACCTGCTGCGCCTGGA GGACGGCGCCGACATCGCCAAGGGCCGCACCAAGTGGCGCCCCAAGAACGCCGGCACCA 15 ACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCATCTCCTGA SEQ ID NO: 25 Cuphea PSR23 (Cu) FATB3 amino acid sequence MVVAAATSAFFPVPAPGTSPKPGKSGNWPSSLSPTFKPKSIPNAGFQVKANASAHPKANGSAV NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRPDMLVD 20 SVGLKCIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGM CKNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSQSGKIGMASDWLISDCNTGEILIRATSVW AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDQKLHKFDVKTGDSIRKGLTPRWNDLD VNQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAVDPSENGGRSQYK HLLRLEDGTDIVKSRTEWRPKNAGTNGAISTSTAKTSNGNSVS* 25 SEQ ID NO: 26 Cuphea PSR23 (Cu) FATB3 coding DNA sequence ATGGTGGTGGCTGCAGCAACTTCTGCATTCTTCCCCGTTCCAGCCCCGGGAACCTCCCCTA AACCCGGGAAGTCCGGCAACTGGCCATCGAGCTTGAGCCCTACCTTCAAGCCCAAGTCAA TCCCCAATGCCGGATTTCAGGTTAAGGCAAATGCCAGTGCCCATCCTAAGGCTAACGGTTC 30 TGCAGTAAATCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCCTCCT CCCCGGGCTTTCCTTAACCAGTTGCCTGATTGGAGTATGCTTCTGACTGCAATCACGACCG TCTTCGTGGCGGCAGAGAAGCAGTGGACTATGCTTGATAGGAAATCTAAGAGGCCTGACA TGCTCGTGGACTCGGTTGGGTTGAAGTGTATTGTTCGGGATGGGCTCGTGTCCAGACAGAG TTTTTTGATTAGATCTTATGAAATAGGCGCTGATCGAACAGCCTCTATAGAGACGCTGATG 35 AACCACTTGCAGGAAACATCTATCAATCATTGTAAGAGTTTGGGTCTTCTCAATGACGGCT TTGGTCGTACTCCTGGGATGTGTAAAAACGACCTCATTTGGGTGCTTACAAAAATGCAGAT CATGGTGAATCGCTACCCAACTTGGGGCGATACTGTTGAGATCAATACCTGGTTCTCTCAG TCGGGGAAAATCGGTATGGCTAGCGATTGGCTAATAAGTGATTGCAACACAGGAGAAATT CTTATAAGAGCAACGAGCGTGTGGGCTATGATGAATCAAAAGACGAGAAGATTCTCAAGA 40 CTTCCATACGAGGTTCGCCAGGAGTTAACGCCTCATTTTGTGGACTCTCCTCATGTCATTG AAGACAATGATCAGAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTCGCAAGG GTCTAACTCCGAGGTGGAACGACTTGGATGTGAATCAGCACGTAAGCAACGTGAAGTACA TTGGGTGGATTCTCGAGAGTATGCCAATAGAAGTTTTGGAGACACAGGAGCTATGCTCTCT CACCGTAGAATATAGGCGGGAATGCGGAATGGACAGTGTGCTGGAGTCCGTGACTGCTGT 45 GGATCCCTCAGAAAATGGAGGCCGGTCTCAGTACAAGCACCTTCTGCGGCTTGAGGATGG GACTGATATCGTGAAGAGCAGAACTGAGTGGCGACCGAAGAATGCAGGAACTAACGGGG CGATATCAACATCAACAGCAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 27 Cuphea PSR23 (Cu) FATB3 coding DNA sequence codon optimized for Prototheca moriformis 50 ATGGTGGTGGCCGCCGCCACCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCTCCCCCA AGCCCGGCAAGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCACCTTCAAGCCCAAGTCCAT CCCCAACGCCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC 55 WO 2014/151904 PCT/US2014/026644 CGCCGTGAACCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCC CCCCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGACCGCCATCACCACCG TGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCGACA TGCTGGTGGACTCCGTGGGCCTGAAGTGCATCGTGCGCGACGGCCTGGTGTCCCGCCAGT 5 CCTTCCTGATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGAT GAACCACCTGCAGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGG CTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCA GATCATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACACCTGGTTCTC CCAGTCCGGCAAGATCGGCATGGCCTCCGACTGGCTGATCTCCGACTGCAACACCGGCGA 10 GATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCTCC CGCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTTCGTGGACTCCCCCCACGTG ATCGAGGACAACGACCAGAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCCGC AAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAG TACATCGGCTGGATCCTGGAGTCCATGCCCATCGAGGTGCTGGAGACCCAGGAGCTGTGC 15 TCCCTGACCGTGGAGTACCGCCGCGAGTGCGGCATGGACTCCGTGCTGGAGTCCGTGACC GCCGTGGACCCCTCCGAGAACGGCGGCCGCTCCCAGTACAAGCACCTGCTGCGCCTGGAG GACGGCACCGACATCGTGAAGTCCCGCACCGAGTGGCGCCCCAAGAACGCCGGCACCAA CGGCGCCATCTCCACCTCCACCGCCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 28 20 Cuphea wrightii (Cw) FATB3 amino acid sequence MVVAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSPHPKANGSA VSLKSGSLNTLEDPPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQFTRLDRKSKRPDMLVDW FGSETIVQDGLVFRERFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTSEMC TRDLIWVLTKMQIVVNRYPTWGDTVEINSWFSQSGKIGMGRDWLISDCNTGEILVRATSAWA 25 MMNQKTRRFSKLPCEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPGWNDLDV NQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRRECGRESVVESVTSMNPSKVGDRSQYQH LLRLEDGADIMKGRTEWRPKNAGTNRAIST* SEQ ID NO: 29 Cuphea wrightii (Cw) FATB3 coding DNA sequence 30 ATGGTGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGTTCCAGCACCTAGAACCACGC CTAAACCCGGGAAGTTCGGCAATTGGCCATCGAGCTTGAGCCCGCCCTTCAAGCCCAAGT CAAACCCCAATGGTAGATTTCAGGTTAAGGCAAATGTCAGTCCTCATCCTAAGGCTAACG GTTCTGCAGTAAGTCTAAAGTCTGGCAGCCTCAACACTCTGGAGGACCCTCCGTCGTCCCC TCCTCCTCGGACTTTCCTTAACCAGTTGCCTGATTGGAGTAGGCTTCGGACTGCAATCACG 35 ACCGTCTTCGTGGCGGCAGAGAAGCAGTTCACTAGGCTCGATCGAAAATCTAAGAGGCCT GACATGCTAGTGGACTGGTTTGGGTCAGAGACTATTGTTCAGGATGGGCTCGTGTTCAGA GAGAGATTTTCGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAGACG CTGATGAACCACTTGCAGGACACATCTCTGAATCATTGTAAGAGTGTGGGTCTTCTCAATG ACGGCTTTGGTCGTACCTCGGAGATGTGTACAAGAGACCTCATTTGGGTGCTTACAAAAAT 40 GCAGATCGTGGTGAATCGCTATCCAACTTGGGGCGATACTGTCGAGATCAATAGCTGGTT CTCCCAGTCGGGGAAAATCGGTATGGGTCGCGATTGGCTAATAAGTGATTGCAACACAGG AGAAATTCTTGTAAGAGCAACGAGCGCTTGGGCCATGATGAATCAAAAGACGAGAAGATT CTCAAAACTTCCATGCGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCTCCTCCT GTCATTGAAGACAATGATCGGAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTT 45 GCAAGGGTCTAACTCCGGGGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGA AGTACATTGGGTGGATTCTCGAGAGTATGCCTACAGAAGTTTTGGAGACCCAGGAGCTAT GCTCTCTCACCCTTGAATATAGGCGGGAATGTGGAAGGGAAAGTGTGGTAGAGTCCGTGA CCTCTATGAATCCCTCAAAAGTTGGAGACCGGTCTCAGTACCAACACCTTCTGCGGCTTGA GGATGGGGCTGATATCATGAAGGGCAGAACTGAGTGGAGACCAAAGAATGCAGGAACCA 50 ACCGGGCGATATCAACATGA SEQ ID NO: 30 Cuphea wrightii (Cw) FATB3 coding DNA sequence codon optimized for Prototheca moriformis 56 WO 2014/151904 PCT/US2014/026644 ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCCCCCGCACCACCC CCAAGCCCGGCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCCCCCCCTTCAAGCCCAAGTC CAACCCCAACGGCCGCTTCCAGGTGAAGGCCAACGTGTCCCCCCACCCCAAGGCCAACGG CTCCGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCCTGGAGGACCCCCCCTCCTCCCCC 5 CCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGTCCCGCCTGCGCACCGCCATCACCA CCGTGTTCGTGGCCGCCGAGAAGCAGTTCACCCGCCTGGACCGCAAGTCCAAGCGCCCCG ACATGCTGGTGGACTGGTTCGGCTCCGAGACCATCGTGCAGGACGGCCTGGTGTTCCGCG AGCGCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCT GATGAACCACCTGCAGGACACCTCCCTGAACCACTGCAAGTCCGTGGGCCTGCTGAACGA 10 CGGCTTCGGCCGCACCTCCGAGATGTGCACCCGCGACCTGATCTGGGTGCTGACCAAGAT GCAGATCGTGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACTCCTGGTT CTCCCAGTCCGGCAAGATCGGCATGGGCCGCGACTGGCTGATCTCCGACTGCAACACCGG CGAGATCCTGGTGCGCGCCACCTCCGCCTGGGCCATGATGAACCAGAAGACCCGCCGCTT CTCCAAGCTGCCCTGCGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCCCCCCC 15 CGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCAT CTGCAAGGGCCTGACCCCCGGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGT GAAGTACATCGGCTGGATCCTGGAGTCCATGCCCACCGAGGTGCTGGAGACCCAGGAGCT GTGCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGGTGGAGTCCGT GACCTCCATGAACCCCTCCAAGGTGGGCGACCGCTCCCAGTACCAGCACCTGCTGCGCCT 20 GGAGGACGGCGCCGACATCATGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA CCAACCGCGCCATCTCCACCTGA SEQ ID NO: 31 Cuphea wrightii (Cw) FATB4a amino acid sequence MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPPKINGSSVGL 25 KSGGFKTQEDSPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPF GLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSNDGFGRTPEMYK RDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSVW VMMNQKTRRLSKIPDEVRNEIEPHFVDSAPVVEDDDRKLPKLDENTADSIRKGLTPRWNDLD VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSAEGYASRFQH 30 LLRLEDGGEIVKARTEWRPKNAGINGVVPSEESSPGDFF* SEQ ID NO: 32 Cuphea wrightii (Cw) FATB4a coding DNA sequence TTGGTGGCTACCGCTGCAAGTTCTGCATTTTTCCCCGTGCCATCCGCCGACACCTCCTCCTC GAGACCCGGAAAGCTCGGCAGTGGACCATCGAGCTTGAGCCCCCTCAAGCCCAAATCGAT 35 CCCCAATGGCGGCTTGCAGGTTAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTC CTCGGTCGGTCTAAAGTCGGGCGGTTTCAAGACTCAGGAAGACTCTCCTTCGGCCCCTCCT CCGCGGACTTTTATCAACCAGTTGCCTGATTGGAGTATGCTTCTTGCTGCAATCACTACTG TCTTCTTGGCTGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCTAAGAGGCCTGACA TGCTCGTGGACCCGTTCGGATTGGGAAGTATTGTTCAGGATGGGCTTGTGTTCAGGCAGAA 40 TTTTTCAATTAGGTCCTACGAAATAGGCGCCGATCGAACTGCGTCTATAGAGACGGTGATG AACCATTTGCAGGAAACAGCTCTCAATCATGTCAAGATTGCTGGGCTTTCTAATGACGGCT TTGGTCGTACTCCTGAGATGTATAAAAGAGACCTTATTTGGGTTGTTGCAAAAATGCAGGT CATGGTTAACCGCTATCCTACTTGGGGTGACACGGTTGAAGTGAATACTTGGGTTGCCAAG TCAGGGAAAAATGGTATGCGTCGTGACTGGCTCATAAGTGATTGCAATACTGGAGAGATT 45 CTTACAAGAGCATCAAGCGTGTGGGTCATGATGAATCAAAAGACAAGAAGATTGTCAAAA ATTCCAGATGAGGTTCGAAATGAGATAGAGCCTCATTTTGTGGACTCTGCTCCCGTCGTTG AAGATGATGATCGGAAACTTCCCAAGCTGGATGAGAACACTGCTGACTCCATCCGCAAGG GTCTAACTCCGAGGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTACA TCGGATGGATTCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGCTCCCT 50 GACCCTGGAATACAGGCGGGAATGTGGAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTGT CGACCCGTCTGCAGAGGGCTATGCGTCCCGGTTTCAGCACCTTCTGCGGCTTGAGGATGGA GGTGAGATCGTGAAGGCGAGAACTGAGTGGCGACCCAAGAATGCTGGAATCAATGGGGT GGTACCATCCGAGGAGTCCTCACCTGGAGACTTCTTTTAG 57 WO 2014/151904 PCT/US2014/026644 SEQ ID NO: 33 Cuphea wrightii (Cw) FATB4a coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCGCCGACACCTCCTCCTC CCGCCCCGGCAAGCTGGGCTCCGGCCCCTCCTCCCTGTCCCCCCTGAAGCCCAAGTCCATC 5 CCCAACGGCGGCCTGCAGGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCC TCCGTGGGCCTGAAGTCCGGCGGCTTCAAGACCCAGGAGGACTCCCCCTCCGCCCCCCCC CCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGGCCGCCATCACCACCG TGTTCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAAGCGCCCCGACA TGCTGGTGGACCCCTTCGGCCTGGGCTCCATCGTGCAGGACGGCCTGGTGTTCCGCCAGAA 10 CTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATG AACCACCTGCAGGAGACCGCCCTGAACCACGTGAAGATCGCCGGCCTGTCCAACGACGGC TTCGGCCGCACCCCCGAGATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAG GTGATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCC AAGTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAG 15 ATCCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGACCCGCCGCCTGTCC AAGATCCCCGACGAGGTGCGCAACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCCGTG GTGGAGGACGACGACCGCAAGCTGCCCAAGCTGGACGAGAACACCGCCGACTCCATCCG CAAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGA AGTACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGT 20 GCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGA CCGCCGTGGACCCCTCCGCCGAGGGCTACGCCTCCCGCTTCCAGCACCTGCTGCGCCTGGA GGACGGCGGCGAGATCGTGAAGGCCCGCACCGAGTGGCGCCCCAAGAACGCCGGCATCA ACGGCGTGGTGCCCTCCGAGGAGTCCTCCCCCGGCGACTTCTTCTGA SEQ ID NO: 34 25 Cuphea wrightii (Cw) FATB4b amino acid sequence MVATAASSAFFPVPSADTSSSRPGKLGNGPSSLSPLKPKSIPNGGLQVKANASAPPKINGSSVGL KSGSFKTQEDAPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPF GLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSSDGFGRTPAMSK RDLIWVVAKMQVMVNRYPAWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSV 30 WVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPVVEDDDRKLPKLDENTADSIRKGLTPRWNDL DVNQHVNNVKYIGWILESTPAEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGEGDGSKF QHLLRLEDGGEIVKARTEWRPKNAGINGVVPSEESSPGGDFF* SEQ ID NO: 35 Cuphea wrightii (Cw) FATB4b coding DNA sequence 35 TTGGTGGCTACCGCTGCAAGTTCTGCATTTTTCCCCGTACCATCCGCCGACACCTCCTCATC GAGACCCGGAAAGCTCGGCAATGGGCCATCGAGCTTGAGCCCCCTCAAGCCGAAATCGAT CCCCAATGGCGGGTTGCAGGTTAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTC CTCGGTCGGTCTGAAGTCGGGCAGTTTCAAGACTCAGGAAGACGCTCCTTCGGCCCCTCCT CCTCGGACTTTTATCAACCAGTTGCCTGATTGGAGTATGCTTCTTGCTGCAATCACTACTGT 40 CTTCTTGGCTGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCTAAGAGGCCTGACAT GCTTGTCGACCCGTTCGGATTGGGAAGTATTGTTCAGGATGGGCTTGTTTTCAGGCAGAAT TTCTCGATTAGGTCCTACGAAATAGGCGCTGATCGCACTGCGTCTATAGAGACGGTGATG AACCATTTGCAGGAAACAGCTCTCAATCATGTTAAGATTGCTGGGCTTTCTAGTGATGGCT TTGGTCGTACTCCTGCGATGTCTAAACGGGACCTCATTTGGGTTGTTGCGAAAATGCAGGT 45 CATGGTTAACCGCTACCCTGCTTGGGGTGACACGGTTGAAGTGAATACTTGGGTTGCCAA GTCAGGGAAAAATGGTATGCGTCGTGACTGGCTCATAAGTGATTGCAACACTGGAGAGAT TCTTACAAGAGCATCAAGCGTGTGGGTCATGATGAATCAAAAGACAAGAAGATTGTCAAA AATTCCAGATGAGGTTCGAAATGAGATAGAGCCTCATTTTGTGGACTCTGCGCCCGTCGTT GAAGACGATGACCGGAAACTTCCCAAGCTGGATGAGAACACTGCTGACTCCATCCGCAAG 50 GGTCTAACTCCGAGGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTAC ATTGGGTGGATTCTTGAGAGTACTCCAGCAGAAGTTCTGGAGACCCAGGAATTATGTTCCC TGACCCTGGAATACAGGCGGGAATGTGGAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTG TAGATCCGTCTGGAGAGGGCGATGGGTCCAAGTTCCAGCACCTTCTGCGGCTTGAGGATG 58 WO 2014/151904 PCT/US2014/026644 GAGGTGAGATCGTGAAGGCGAGAACTGAGTGGCGACCAAAGAATGCTGGAATCAATGGG GTGGTACCATCCGAGGAGTCCTCACCTGGTGGAGACTTCTTTTAA SEQ ID NO: 36 Cuphea wrightii (Cw) FATB4b coding DNA sequence codon optimized for Prototheca moriformis 5 ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCGCCGACACCTCCTCCTC CCGCCCCGGCAAGCTGGGCAACGGCCCCTCCTCCCTGTCCCCCCTGAAGCCCAAGTCCATC CCCAACGGCGGCCTGCAGGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCC TCCGTGGGCCTGAAGTCCGGCTCCTTCAAGACCCAGGAGGACGCCCCCTCCGCCCCCCCCC CCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGGCCGCCATCACCACCGT 10 GTTCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAAGCGCCCCGACAT GCTGGTGGACCCCTTCGGCCTGGGCTCCATCGTGCAGGACGGCCTGGTGTTCCGCCAGAA CTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATG AACCACCTGCAGGAGACCGCCCTGAACCACGTGAAGATCGCCGGCCTGTCCTCCGACGGC TTCGGCCGCACCCCCGCCATGTCCAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAG 15 GTGATGGTGAACCGCTACCCCGCCTGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCC AAGTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAG ATCCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGACCCGCCGCCTGTCC AAGATCCCCGACGAGGTGCGCAACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCCGTG GTGGAGGACGACGACCGCAAGCTGCCCAAGCTGGACGAGAACACCGCCGACTCCATCCG 20 CAAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGA AGTACATCGGCTGGATCCTGGAGTCCACCCCCGCCGAGGTGCTGGAGACCCAGGAGCTGT GCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGA CCGCCGTGGACCCCTCCGGCGAGGGCGACGGCTCCAAGTTCCAGCACCTGCTGCGCCTGG AGGACGGCGGCGAGATCGTGAAGGCCCGCACCGAGTGGCGCCCCAAGAACGCCGGCATC 25 AACGGCGTGGTGCCCTCCGAGGAGTCCTCCCCCGGCGGCGACTTCTTCTGA SEQ ID NO: 37 Cuphea wrightii (Cw) FATB5 amino acid sequence MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPKANGSAVNL KSGSLETPPRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRIFQDGVFFRQSFSI 30 RSYEIGVDRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWVVTKIQVEVNRYP TWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNQNTRRLSKFPYEVRQE IAPHFVDSAPVIEDDQKLQKLDVKTGDSIRDGLTPRWNDLDVNQHVNNVKYIGWILKSVPIEV FETQELCGVTLEYRRECGRDSVLESVTAMDPAKEGDRCVYQHLLRLEDGADITIGRTEWRPK NAGANGAMSSGKTSNGNCLIEGRGWQPFRVVRLIF* 35 SEQ ID NO: 38 Cuphea wrightii (Cw) FATB5 coding DNA sequence ATGGTGGCTGCCGCAGCAAGTTCTGCATTCTTCTCTGTTCCAACCCCGGGAACGCCCCCTA AACCCGGGAAGTTCGGTAACTGGCCATCGAGCTTGAGCGTCCCCTTCAAGCCCGACAATG GTGGCTTTCATGTCAAGGCAAACGCCAGTGCCCATCCTAAGGCTAATGGTTCTGCGGTAA 40 ATCTAAAGTCTGGCAGCCTCGAGACTCCTCCTCGGAGTTTCATTAACCAGCTGCCGGACTT GAGTGTGCTTCTGTCCAAAATCACGACTGTCTTCGGGGCGGCTGAGAAGCAGTGGAAGAG GCCCGGCATGCTCGTGGAACCGTTTGGGGTTGACAGGATTTTTCAGGATGGTGTTTTTTTC AGACAGAGTTTTTCTATCAGGTCTTACGAAATAGGCGTTGATCGAACAGCCTCGATAGAG ACACTGATGAACATCTTCCAGGAAACATCTTTGAATCATTGCAAGAGTATCGGTCTTCTCA 45 ACGATGGCTTTGGTCGTACTCCTGAGATGTGTAAGAGGGACCTCATTTGGGTGGTTACGAA AATTCAGGTCGAGGTGAATCGCTATCCTACTTGGGGTGACACTATCGAAGTCAATACTTGG GTCTCGGAGTCGGGGAAAAACGGTATGGGTCGGGATTGGCTGATAAGTGATTGCCGTACT GGAGAGATTCTTATAAGAGCAACGAGCGTGTGGGCGATGATGAATCAAAACACGAGAAG ATTGTCAAAATTTCCATATGAGGTTCGACAGGAGATAGCGCCTCATTTTGTGGACTCTGCT 50 CCTGTCATTGAAGACGATCAAAAGTTGCAGAAGCTTGATGTGAAGACAGGTGATTCCATT CGCGATGGTCTAACTCCGAGATGGAATGACTTGGATGTCAATCAACACGTTAACAATGTG AAGTACATTGGATGGATTCTCAAGAGTGTTCCAATAGAAGTTTTCGAGACACAGGAGCTA 59 WO 2014/151904 PCT/US2014/026644 TGCGGCGTCACACTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCAGTG ACCGCTATGGATCCAGCAAAAGAGGGAGACCGGTGTGTGTACCAGCACCTTCTTCGGCTT GAGGATGGAGCTGATATCACTATAGGCAGAACCGAGTGGCGGCCGAAGAATGCAGGAGC CAATGGTGCAATGTCATCAGGAAAGACTTCAAATGGAAACTGTCTCATAGAAGGAAGGGG 5 TTGGCAACCTTTCCGAGTTGTGCGTTTAATTTTCTGA SEQ ID NO: 39 Cuphea wrightii (Cw) FATB5 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCTCCGTGCCCACCCCCGGCACCCCCCCCA AGCCCGGCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCGTGCCCTTCAAGCCCGACAACGG 10 CGGCTTCCACGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGAA CCTGAAGTCCGGCTCCCTGGAGACCCCCCCCCGCTCCTTCATCAACCAGCTGCCCGACCTG TCCGTGCTGCTGTCCAAGATCACCACCGTGTTCGGCGCCGCCGAGAAGCAGTGGAAGCGC CCCGGCATGCTGGTGGAGCCCTTCGGCGTGGACCGCATCTTCCAGGACGGCGTGTTCTTCC GCCAGTCCTTCTCCATCCGCTCCTACGAGATCGGCGTGGACCGCACCGCCTCCATCGAGAC 15 CCTGATGAACATCTTCCAGGAGACCTCCCTGAACCACTGCAAGTCCATCGGCCTGCTGAAC GACGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAG ATCCAGGTGGAGGTGAACCGCTACCCCACCTGGGGCGACACCATCGAGGTGAACACCTGG GTGTCCGAGTCCGGCAAGAACGGCATGGGCCGCGACTGGCTGATCTCCGACTGCCGCACC GGCGAGATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAACACCCGCCGC 20 CTGTCCAAGTTCCCCTACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACTCCGCCC CCGTGATCGAGGACGACCAGAAGCTGCAGAAGCTGGACGTGAAGACCGGCGACTCCATC CGCGACGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTG AAGTACATCGGCTGGATCCTGAAGTCCGTGCCCATCGAGGTGTTCGAGACCCAGGAGCTG TGCGGCGTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTG 25 ACCGCCATGGACCCCGCCAAGGAGGGCGACCGCTGCGTGTACCAGCACCTGCTGCGCCTG GAGGACGGCGCCGACATCACCATCGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCGC CAACGGCGCCATGTCCTCCGGCAAGACCTCCAACGGCAACTGCCTGATCGAGGGCCGCGG CTGGCAGCCCTTCCGCGTGGTGCGCCTGATCTTCTGA SEQ ID NO: 40 30 Cuphea heterophylla (Cht) FATB 1 a amino acid sequence MVAAAASSAFFSVPTPGTSTKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASAHPKANGSA VNLKSGSLETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRPGMLVEPFGVDRI FQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWV VTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNRKT 35 RRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGDSIRKGLTPRWNDLDVNQHVNNV KYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVLESVTAMDTAKEGDRSLYQHLLRLEDG ADITIGRTEWRPKNAGANGAISTGKTSNENSVS* SEQ ID NO: 41 Cuphea heterophylla (Cht) FATB1 a coding DNA sequence 40 ATGGTGGCTGCCGCAGCAAGTTCTGCATTCTTCTCCGTTCCAACCCCGGGAACCTCCACTA AACCCGGGAACTTCGGCAATTGGCCATCGAGCTTGAGCGTCCCCTTCAAGCCCGAATCAA ACCACAATGGTGGCTTTCGGGTCAAGGCAAACGCCAGTGCTCATCCTAAGGCTAACGGTT CTGCAGTAAATCTAAAGTCTGGCAGCCTCGAGACTCAGGAGGACACTTCATCGTCGTCCC CTCCTCCTCGGACTTTTATTAAGCAGTTGCCCGACTGGGGTATGCTTCTGTCCAAAATCAC 45 GACTGTCTTCGGGGCGGCTGAGAGGCAGTGGAAGAGGCCCGGCATGCTTGTGGAACCGTT TGGGGTTGACAGGATTTTTCAGGATGGGGTTTTTTTCAGACAGAGTTTTTCGATCAGGTCT TACGAAATAGGCGCTGATCGAACAGCCTCAATAGAGACGCTGATGAACATCTTCCAGGAA ACATCTCTGAATCATTGTAAGAGTATCGGTCTTCTCAATGACGGCTTTGGTCGTACTCCTG AGATGTGTAAGAGGGACCTCATTTGGGTGGTTACGAAAATTCAGGTCGAGGTGAATCGCT 50 ATCCTACTTGGGGTGATACTATTGAGGTCAATACTTGGGTCTCAGAGTCGGGGAAAAACG GTATGGGTCGTGATTGGCTGATAAGCGATTGCCGTACCGGAGAAATTCTTATAAGAGCAA CGAGCGTGTGGGCTATGATGAATCGAAAGACGAGAAGATTGTCAAAATTTCCATATGAGG 60 WO 2014/151904 PCT/US2014/026644 TTCGACAGGAGATAGCGCCTCATTTTGTGGACTCTGCTCCTGTCATTGAAGACGATAAAAA ATTGCACAAGCTTGATGTTAAGACGGGTGATTCCATTCGCAAGGGTCTAACTCCAAGGTG GAATGACTTGGATGTCAATCAGCACGTTAACAATGTGAAGTACATTGGGTGGATTCTCAA GAGTGTTCCAGCAGAAGTTTTCGAGACCCAGGAGCTATGCGGAGTCACCCTTGAGTACAG 5 GCGGGAATGTGGAAGGGACAGTGTGCTGGAGTCCGTGACCGCTATGGATACCGCAAAAG AGGGAGACCGGTCTCTGTACCAGCACCTTCTTCGGCTTGAGGATGGGGCTGATATCACCAT AGGCAGAACCGAGTGGCGGCCGAAGAATGCAGGAGCCAATGGGGCAATATCAACAGGAA AGACTTCAAATGAAAACTCTGTCTCTTAG SEQ ID NO: 42 10 Cuphea heterophylla (Cht) FATB 1 a coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCTCCGTGCCCACCCCCGGCACCTCCACCA AGCCCGGCAACTTCGGCAACTGGCCCTCCTCCCTGTCCGTGCCCTTCAAGCCCGAGTCCAA CCACAACGGCGGCTTCCGCGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC 15 CGCCGTGAACCTGAAGTCCGGCTCCCTGGAGACCCAGGAGGACACCTCCTCCTCCTCCCCC CCCCCCCGCACCTTCATCAAGCAGCTGCCCGACTGGGGCATGCTGCTGTCCAAGATCACCA CCGTGTTCGGCGCCGCCGAGCGCCAGTGGAAGCGCCCCGGCATGCTGGTGGAGCCCTTCG GCGTGGACCGCATCTTCCAGGACGGCGTGTTCTTCCGCCAGTCCTTCTCCATCCGCTCCTA CGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGAACATCTTCCAGGAGAC 20 CTCCCTGAACCACTGCAAGTCCATCGGCCTGCTGAACGACGGCTTCGGCCGCACCCCCGA GATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGATCCAGGTGGAGGTGAACCGCTA CCCCACCTGGGGCGACACCATCGAGGTGAACACCTGGGTGTCCGAGTCCGGCAAGAACGG CATGGGCCGCGACTGGCTGATCTCCGACTGCCGCACCGGCGAGATCCTGATCCGCGCCAC CTCCGTGTGGGCCATGATGAACCGCAAGACCCGCCGCCTGTCCAAGTTCCCCTACGAGGT 25 GCGCCAGGAGATCGCCCCCCACTTCGTGGACTCCGCCCCCGTGATCGAGGACGACAAGAA GCTGCACAAGCTGGACGTGAAGACCGGCGACTCCATCCGCAAGGGCCTGACCCCCCGCTG GAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGATCCTGAA GTCCGTGCCCGCCGAGGTGTTCGAGACCCAGGAGCTGTGCGGCGTGACCCTGGAGTACCG CCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGGACACCGCCAAGGA 30 GGGCGACCGCTCCCTGTACCAGCACCTGCTGCGCCTGGAGGACGGCGCCGACATCACCAT CGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCGCCAACGGCGCCATCTCCACCGGCAA GACCTCCAACGAGAACTCCGTGTCCTGA SEQ ID NO: 43 Cuphea heterophylla (Cht) FATBib (P16S, T20P, G94S, G105W, S293F, L305F variant) amino acid 35 sequence MVAAAASSAFFSVPTSGTSPKPGNFGNWPSSLSVPFKPES SHNGGFQVKANASAHPKANGSAV NLKSGSLETQEDTSSSSPPPRTFIKQLPDWSMLLSKITTVFWAAERQWKRPGMLVEPFGVDRIF QDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWVV TKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNRKTRR 40 LSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGDFIRKGLTPRWNDFDVNQHVNNVKYI GWILKSVPAEVFETQELCGVTLEYRRECGRDSVLESVTAMDTAKEGDRSLYQHLLRLEDGADI TIGRTEWRPKNAGANGAISTGKTSNENSVS* SEQ ID NO: 44 Cuphea heterophylla (Cht) FATB1b(P16S, T20P, G94S, G105W, S293F, L305F variant) coding DNA 45 sequence ATGGTGGCTGCCGCAGCAAGTTCTGCATTCTTCTCCGTTCCAACCTCGGGAACCTCCCCTA AACCCGGGAACTTCGGCAATTGGCCATCGAGCTTGAGCGTCCCCTTCAAGCCCGAATCAA GCCACAATGGTGGCTTTCAGGTCAAGGCAAACGCCAGTGCCCATCCTAAGGCTAACGGTT CTGCAGTAAATCTAAAGTCTGGCAGCCTCGAGACTCAGGAGGACACTTCATCGTCGTCCC 50 CTCCTCCTCGGACTTTTATTAAGCAGTTGCCCGACTGGAGTATGCTTCTGTCCAAAATCAC GACTGTCTTCTGGGCGGCTGAGAGGCAGTGGAAGAGGCCCGGCATGCTTGTGGAACCGTT TGGGGTTGACAGGATTTTTCAGGATGGGGTTTTTTTCAGACAGAGTTTTTCGATCAGGTCT 61 WO 2014/151904 PCT/US2014/026644 TACGAAATAGGCGCTGATCGAACAGCCTCAATAGAGACGCTGATGAACATCTTCCAGGAA ACATCTCTGAATCATTGTAAGAGTATCGGTCTTCTCAATGACGGCTTTGGTCGTACTCCTG AGATGTGTAAGAGGGACCTCATTTGGGTGGTTACGAAAATTCAGGTCGAGGTGAATCGCT ATCCTACTTGGGGTGATACTATTGAGGTCAATACTTGGGTCTCAGAGTCGGGGAAAAACG 5 GTATGGGTCGTGATTGGCTGATAAGCGATTGCCGTACCGGAGAAATTCTTATAAGAGCAA CGAGCGTGTGGGCTATGATGAATCGAAAGACGAGAAGATTGTCAAAATTTCCATATGAGG TTCGACAGGAGATAGCGCCTCATTTTGTGGACTCTGCTCCTGTCATTGAAGACGATAAAAA ATTGCACAAGCTTGATGTTAAGACGGGTGATTTCATTCGCAAGGGTCTAACTCCAAGGTG GAATGACTTTGATGTCAATCAGCACGTTAACAATGTGAAGTACATTGGGTGGATTCTCAA 10 GAGTGTTCCAGCAGAAGTTTTCGAGACCCAGGAGCTATGCGGAGTCACCCTTGAGTATAG GCGGGAATGTGGAAGGGACAGTGTGCTGGAGTCCGTGACCGCTATGGATACCGCAAAAG AGGGAGACCGGTCTCTGTACCAGCACCTTCTTCGGCTTGAGGATGGGGCTGATATCACCAT AGGCAGAACCGAGTGGCGGCCGAAGAATGCAGGAGCCAATGGGGCAATATCAACAGGAA AGACTTCAAATGAAAACTCTGTCTCTTAG 15 SEQ ID NO: 45 Cuphea heterophylla (Cht) FATBib (P16S, T20P, G94S, G105W, S293F, L305F variant) coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCTCCGTGCCCACCTCCGGCACCTCCCCCA AGCCCGGCAACTTCGGCAACTGGCCCTCCTCCCTGTCCGTGCCCTTCAAGCCCGAGTCCTC 20 CCACAACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC CGCCGTGAACCTGAAGTCCGGCTCCCTGGAGACCCAGGAGGACACCTCCTCCTCCTCCCCC CCCCCCCGCACCTTCATCAAGCAGCTGCCCGACTGGTCCATGCTGCTGTCCAAGATCACCA CCGTGTTCTGGGCCGCCGAGCGCCAGTGGAAGCGCCCCGGCATGCTGGTGGAGCCCTTCG GCGTGGACCGCATCTTCCAGGACGGCGTGTTCTTCCGCCAGTCCTTCTCCATCCGCTCCTA 25 CGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGAACATCTTCCAGGAGAC CTCCCTGAACCACTGCAAGTCCATCGGCCTGCTGAACGACGGCTTCGGCCGCACCCCCGA GATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGATCCAGGTGGAGGTGAACCGCTA CCCCACCTGGGGCGACACCATCGAGGTGAACACCTGGGTGTCCGAGTCCGGCAAGAACGG CATGGGCCGCGACTGGCTGATCTCCGACTGCCGCACCGGCGAGATCCTGATCCGCGCCAC 30 CTCCGTGTGGGCCATGATGAACCGCAAGACCCGCCGCCTGTCCAAGTTCCCCTACGAGGT GCGCCAGGAGATCGCCCCCCACTTCGTGGACTCCGCCCCCGTGATCGAGGACGACAAGAA GCTGCACAAGCTGGACGTGAAGACCGGCGACTTCATCCGCAAGGGCCTGACCCCCCGCTG GAACGACTTCGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGATCCTGAA GTCCGTGCCCGCCGAGGTGTTCGAGACCCAGGAGCTGTGCGGCGTGACCCTGGAGTACCG 35 CCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGGACACCGCCAAGGA GGGCGACCGCTCCCTGTACCAGCACCTGCTGCGCCTGGAGGACGGCGCCGACATCACCAT CGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCGCCAACGGCGCCATCTCCACCGGCAA GACCTCCAACGAGAACTCCGTGTCCTGA SEQ ID NO: 46 40 Cuphea heterophylla (Cht) FATB2b amino acid sequence MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSA VSLKSGSLNTQEGTSSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQLTMLDRKSKKPDMHVD WFGLEIIVQDGLVFRESFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTPEM CKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWA 45 MMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPEWNDLDV NQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGDRSQYQ HLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS* SEQ ID NO: 47 Cuphea heterophylla (Cht) FATB2b coding DNA sequence 50 ATGGTGGTGGCTGCTGCAGCAAGCTCTGCATTCTTCCCTGTTCCGGCATCTGGAACCTCCC CTAAACCCGGGAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGT CAAACCCCAGTGGTGGATTTCAGGTTAAGGCAAATGCCAGTGCTCATCCTAAGGCTAACG 62 WO 2014/151904 PCT/US2014/026644 GTTCCGCAGTAAGTCTAAAGTCTGGCAGCCTCAACACTCAGGAGGGCACTTCGTCGTCCCC TCCTCCTCGGACTTTCCTTAACCAGTTGCCTGATTGGAGTAGGCTTCGGACTGCAATCACG ACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAAGTCTAAGAAGCCT GACATGCACGTGGACTGGTTTGGGTTGGAGATTATTGTTCAGGATGGGCTCGTGTTCAGAG 5 AGAGTTTTTCGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAAACGTT GATGAACCATTTGCAGGACACATCTTTGAACCATTGTAAGAGTGTGGGTCTTCTCAATGAC GGCTTTGGTCGTACCCCGGAGATGTGTAAAAGGGACCTCATTTGGGTGCTTACAAAAATG CAGATCATGGTGAATCGCTATCCAACTTGGGGCGATACTGTCGAGATCAATAGCTGGTTCT CCCAGTCCGGGAAAATCGGTATGGGTCGCAATTGGCTAATAAGTGATTGCAACACAGGAG 10 AAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCAAAAGACGAGAAGATTCT CAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCCCCTCCTGT CATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTTGC AAGGGTCTAACACCGGAGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAG TACATTGGGTGGATTCTCGAGAGTATGCCAAAAGAAGTTTTGGACACCCAGGAGCTATGC 15 TCTCTCACCCTTGAATATAGGCGGGAATGCGGAAGGGATAGTGTGCTGGAGTCTGTGACC GCTATGGATCCCTCAAAAGTTGGAGACCGATCTCAGTACCAGCACCTTCTGCGGCTTGAA GATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACCAAAGAATGCAGGAACCAA CGGGGCTATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 48 20 Cuphea heterophylla (Cht) FATB2b coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCTCCGGCACCTCCC CCAAGCCCGGCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTC CAACCCCTCCGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGG 25 CTCCGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCCAGGAGGGCACCTCCTCCTCCCCC CCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGTCCCGCCTGCGCACCGCCATCACCA CCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAAGTCCAAGAAGCCCG ACATGCACGTGGACTGGTTCGGCCTGGAGATCATCGTGCAGGACGGCCTGGTGTTCCGCG AGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCT 30 GATGAACCACCTGCAGGACACCTCCCTGAACCACTGCAAGTCCGTGGGCCTGCTGAACGA CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGAT GCAGATCATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACTCCTGGTT CTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACTGGCTGATCTCCGACTGCAACACCGG CGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCAGAAGACCCGCCGCTT 35 CTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCCCCCCC CGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCAT CTGCAAGGGCCTGACCCCCGAGTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGT GAAGTACATCGGCTGGATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCT GTGCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGT 40 GACCGCCATGGACCCCTCCAAGGTGGGCGACCGCTCCCAGTACCAGCACCTGCTGCGCCT GGAGGACGGCACCGACATCATGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA CCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 49 Cuphea heterophylla (Cht) FATB2a (S17P, P21S, T28N, L30P, S33L, G76D, S78P, G137W variant) 45 amino acid sequence MVVAAAASSAFFPVPAPGTTSKPGKFGNWPSSLSPSFKPKSNPNGGFQVKANASAHPKANGS AVSLKSGSLNTKEDTPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQLTMLDRKSKKPDMHV DWFGLEIIVQDWLVFRESFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTPE MCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIW 50 AMMNQKTRRFSKLPNEVRQEIAPHFVDAPPLIEDNDRKLHKFDVKTGDSICKGLTPEWNDLD VNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGDRSQY QHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS* 63 WO 2014/151904 PCT/US2014/026644 SEQ ID NO: 50 Cuphea heterophylla (Cht) FATB2a (S17P, P21S, T28N, L30P, S33L, G76D, S78P, G137W variant) coding DNA sequence ATGGTGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGTTCCAGCACCTGGAACCACGT 5 CTAAACCCGGGAAGTTCGGCAATTGGCCATCGAGCTTGAGCCCTTCCTTCAAGCCCAAGTC AAACCCCAATGGTGGATTTCAGGTTAAGGCAAATGCCAGCGCTCATCCTAAGGCTAACGG GTCTGCAGTAAGTCTAAAGTCTGGCAGCCTCAACACTAAGGAGGACACTCCGTCGTCCCC TCCTCCTCGGACTTTCCTTAACCAGTTGCCTGATTGGAGTAGGCTTCGGACTGCAATCACG ACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAAGTCTAAGAAGCCT 10 GACATGCACGTGGACTGGTTTGGGTTGGAGATTATTGTTCAGGATTGGCTCGTGTTCAGAG AGAGTTTTTCGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAAACGTT GATGAACCATTTGCAGGACACATCTTTGAACCATTGTAAGAGTGTGGGTCTTCTCAATGAC GGCTTTGGTCGTACCCCGGAGATGTGTAAAAGGGACCTCATTTGGGTGCTTACAAAAATG CAGATCATGGTGAATCGCTATCCAACTTGGGGCGATACTGTCGAGATCAATAGCTGGTTCT 15 CCCAGTCCGGGAAAATCGGTATGGGTCGCAATTGGCTAATAAGTGATTGCAACACAGGAG AAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCAAAAGACGAGAAGATTCT CAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCTCCTCATTTTGTGGACGCCCCTCCTCT CATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTTGC AAGGGTCTAACACCGGAGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAG 20 TACATTGGGTGGATTCTCGAGAGTATGCCAAAAGAAGTTTTGGACACCCAGGAGCTATGC TCTCTCACCCTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCTGTGACC GCTATGGATCCCTCAAAAGTTGGAGACCGATCTCAGTACCAGCACCTTCTGCGGCTTGAA GATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACCAAAGAATGCAGGAACCAA CGGGGCGATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG 25 SEQ ID NO: 51 Cuphea heterophylla (Cht) FATB2a (S17P, P21S, T28N, L30P, S33L, G76D, S78P, G137W variant) coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCACCT CCAAGCCCGGCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGCCCAAGTC 30 CAACCCCAACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGG CTCCGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCAAGGAGGACACCCCCTCCTCCCCC CCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGTCCCGCCTGCGCACCGCCATCACCA CCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAAGTCCAAGAAGCCCG ACATGCACGTGGACTGGTTCGGCCTGGAGATCATCGTGCAGGACTGGCTGGTGTTCCGCG 35 AGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCT GATGAACCACCTGCAGGACACCTCCCTGAACCACTGCAAGTCCGTGGGCCTGCTGAACGA CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGAT GCAGATCATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACTCCTGGTT CTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACTGGCTGATCTCCGACTGCAACACCGG 40 CGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCAGAAGACCCGCCGCTT CTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCCCCCCC CCTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCAT CTGCAAGGGCCTGACCCCCGAGTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGT GAAGTACATCGGCTGGATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCT 45 GTGCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGT GACCGCCATGGACCCCTCCAAGGTGGGCGACCGCTCCCAGTACCAGCACCTGCTGCGCCT GGAGGACGGCACCGACATCATGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA CCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 52 50 Cuphea heterophylla (Cht) FATB2c (G76D, S78P variant) amino acid sequence MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSA VSLKSGSLNTKEDTPSSPPPRTFLNQLPDWNRLRTAITTVFVAAEKQLTMLDRKSKKPDMHVD WFGLEIIVQDGLVFRESFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTPEM 64 WO 2014/151904 PCT/US2014/026644 CKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWA MMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPEWNDLDV NQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGDRSQYQ HLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS* 5 SEQ ID NO: 53 Cuphea heterophylla (Cht) FATB2c (G76D, S78P variant) coding DNA sequence ATGGTGGTGGCTGCTGCAGCAAGCTCTGCATTCTTCCCTGTTCCGGCATCTGGAACCTCCC CTAAACCCGGGAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGT CAAACCCCAGTGGTGGATTTCAGGTTAAGGCAAATGCCAGTGCTCATCCTAAGGCTAACG 10 GTTCCGCAGTAAGTCTAAAGTCTGGCAGCCTCAACACTAAGGAGGACACTCCGTCGTCCC CTCCTCCTCGGACTTTCCTTAACCAGTTGCCTGATTGGAATAGGCTTCGGACTGCAATCAC GACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAAGTCTAAGAAGCC TGACATGCACGTGGACTGGTTTGGGTTGGAGATTATTGTTCAGGATGGGCTCGTGTTCAGA GAGAGTTTTTCGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAAACG 15 TTGATGAACCATTTGCAGGACACATCTTTGAACCATTGTAAGAGTGTGGGTCTTCTCAATG ACGGCTTTGGTCGTACCCCGGAGATGTGTAAAAGGGACCTCATTTGGGTGCTTACAAAAA TGCAGATCATGGTGAATCGCTATCCAACTTGGGGCGATACTGTCGAGATCAATAGCTGGTT CTCCCAGTCCGGGAAAATCGGTATGGGTCGCAATTGGCTAATAAGTGATTGCAACACAGG AGAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCAAAAGACGAGAAGATT 20 CTCAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCCCCTCCT GTCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTT GCAAGGGTCTAACACCGGAGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGA AGTACATTGGGTGGATTCTCGAGAGTATGCCAAAAGAAGTTTTGGACACCCAGGAGCTAT GCTCTCTCACCCTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCTGTGA 25 CCGCTATGGATCCCTCAAAAGTTGGGGACCGATCTCAGTACCAGCACCTTCTGCGGCTTGA AGATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACCAAAGAATGCAGGAACCA ACGGGGCTATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 54 Cuphea heterophylla (Cht) FATB2c (G76D, S78P variant) coding DNA sequence codon optimized for 30 Prototheca moriformis ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCTCCGGCACCTCCC CCAAGCCCGGCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTC CAACCCCTCCGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGG CTCCGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCAAGGAGGACACCCCCTCCTCCCCC 35 CCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGAACCGCCTGCGCACCGCCATCACCA CCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAAGTCCAAGAAGCCCG ACATGCACGTGGACTGGTTCGGCCTGGAGATCATCGTGCAGGACGGCCTGGTGTTCCGCG AGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCT GATGAACCACCTGCAGGACACCTCCCTGAACCACTGCAAGTCCGTGGGCCTGCTGAACGA 40 CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGAT GCAGATCATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACTCCTGGTT CTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACTGGCTGATCTCCGACTGCAACACCGG CGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCAGAAGACCCGCCGCTT CTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCCCCCCC 45 CGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCAT CTGCAAGGGCCTGACCCCCGAGTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGT GAAGTACATCGGCTGGATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCT GTGCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGT GACCGCCATGGACCCCTCCAAGGTGGGCGACCGCTCCCAGTACCAGCACCTGCTGCGCCT 50 GGAGGACGGCACCGACATCATGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA CCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA 65 WO 2014/151904 PCT/US2014/026644 SEQ ID NO: 55 Cuphea heterophylla (Cht) FATB2d (S21P, T28N, L30P, S33L, G76D, R97L, H124L, W127L, 1132S, K258N, C303R, E309G, K334T, T386A variant) amino acid sequence MVVAAAASSAFFPVPAPGTTSKPGKFGNWPSSLSPSFKPKSNPNGGFQVKANASAHPKANGS 5 AVSLKSGSLNTQEDTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRPDMLV DLFGLESIVQDGLVFRESYSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTPE MCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIW AMMNQNTRRF SKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSIRKGLTPGWNDLD VNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRRECGRESVLESVTAMNPSKVGDRSQYQ 10 HLLRLEDGADIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS* SEQ ID NO: 56 Cuphea heterophylla (Cht) FATB2d (S21P, T28N, L30P, S33L, G76D, R97L, H124L, W127L, I132S, K258N, C303R, E309G, K334T, T386A variant) coding DNA sequence ATGGTGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGTTCCAGCACCTGGAACCACGT 15 CTAAACCCGGGAAGTTCGGCAATTGGCCATCGAGCTTGAGCCCTTCCTTCAAGCCCAAGTC AAACCCCAATGGTGGATTTCAGGTTAAGGCAAATGCCAGTGCTCATCCTAAGGCTAACGG TTCTGCGGTAAGTCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCCT CCTCCTCGGACATTCCTTAACCAGTTGCCTGATTGGAGTAGGCTTCTGACTGCAATCTCGA CCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAAATCTAAGAGGCCTG 20 ACATGCTCGTGGACTTGTTTGGGTTGGAGAGTATTGTTCAGGATGGGCTCGTGTTCAGAGA GAGTTATTCGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAAACGTT GATGAACCATTTGCAGGACACATCTTTGAACCATTGTAAGAGTGTGGGTCTTCTCAATGAC GGCTTTGGTCGTACCCCGGAGATGTGTAAAAGGGACCTCATTTGGGTGCTTACAAAAATG CAGATCATGGTGAATCGCTATCCAACTTGGGGCGATACTGTCGAGATCAATAGCTGGTTCT 25 CCCAGTCCGGGAAAATCGGTATGGGTCGCAATTGGCTAATAAGTGATTGCAACACAGGAG AAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCAAAATACGAGAAGATTCT CAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTTGACGCTCCTCCTGT CATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTCG CAAGGGTCTAACTCCGGGGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAA 30 GTACATTGGGTGGATTCTCGAGAGTATGCCAACAGAAGTTTTGGAGACCCAGGAGCTATG CTCTCTCACCCTTGAATATAGGCGGGAATGCGGAAGGGAAAGTGTGCTGGAGTCCGTGAC CGCTATGAATCCCTCAAAAGTTGGAGACCGGTCTCAGTACCAGCACCTTCTACGGCTTGAG GATGGGGCTGATATCATGAAGGGCAGAACTGAGTGGCGACCAAAGAATGCAGGAACCAA CGGGGCGATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG 35 SEQ ID NO: 57 Cuphea heterophylla (Cht) FATB2d (S21P, T28N, L30P, S33L, G76D, R97L, H124L, W127L, I132S, K258N, C303R, E309G, K334T, T386A variant) coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCACCT 40 CCAAGCCCGGCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGCCCAAGTC CAACCCCAACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGG CTCCGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCC CCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGTCCCGCCTGCTGACCGCCATCTCCA CCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAAGTCCAAGCGCCCCG 45 ACATGCTGGTGGACCTGTTCGGCCTGGAGTCCATCGTGCAGGACGGCCTGGTGTTCCGCG AGTCCTACTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCT GATGAACCACCTGCAGGACACCTCCCTGAACCACTGCAAGTCCGTGGGCCTGCTGAACGA CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGAT GCAGATCATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACTCCTGGTT 50 CTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACTGGCTGATCTCCGACTGCAACACCGG CGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCAGAACACCCGCCGCTT CTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCCCCCCC CGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCAT 66 WO 2014/151904 PCT/US2014/026644 CCGCAAGGGCCTGACCCCCGGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGT GAAGTACATCGGCTGGATCCTGGAGTCCATGCCCACCGAGGTGCTGGAGACCCAGGAGCT GTGCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCGT GACCGCCATGAACCCCTCCAAGGTGGGCGACCGCTCCCAGTACCAGCACCTGCTGCGCCT 5 GGAGGACGGCGCCGACATCATGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA CCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 58 Cuphea heterophylla (Cht) FATB2e (G76D, R97L, H124L, 1132S, G152S, H165L, T211N, K258N, C303R, E309G, K334T, T386A variant) amino acid sequence 10 MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSA VSLKSGSLNTQEDTSSSPPPQTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRPDMLVD WFGLESIVQDGLVFRESYSIRSYEISADRTASIETVMNLLQETSLNHCKSMGILNDGFGRTPEM CKRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWA MMNQNTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSIRKGLTPGWNDLDV 15 NQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRRECGRDSVLESVTAMNPSKVGDRSQYQ HLLRLEDGADIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS* SEQ ID NO: 59 Cuphea heterophylla (Cht) FATB2e (G76D, R97L, H124L, I132S, G152S, H165L, T211N, K258N, C303R, E309G, K334T, T386A variant) coding DNA sequence 20 ATGGTGGTGGCTGCTGCAGCAAGCTCTGCATTCTTCCCTGTTCCGGCATCTGGAACCTCCC CTAAACCCGGGAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGT CAAACCCCAGTGGTGGATTTCAGGTTAAGGCAAATGCCAGTGCTCATCCTAAGGCTAACG GTTCTGCAGTAAGTCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCC TCCTCCTCAGACATTCCTTAACCAGTTGCCTGATTGGAGTAGGCTTCTGACAGCAATCTCG 25 ACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAAATCTAAAAGGCCT GACATGCTCGTGGACTGGTTTGGGTTGGAGAGTATTGTTCAGGATGGGCTCGTGTTCAGAG AGAGTTATTCGATCAGGTCTTACGAAATAAGCGCTGATCGAACAGCCTCTATAGAGACGG TGATGAACCTCTTGCAGGAAACATCTCTCAATCATTGTAAGAGTATGGGTATTCTCAATGA CGGCTTTGGTCGTACCCCGGAGATGTGCAAAAGGGACCTCATTTGGGTGCTTACAAAAAT 30 GCAGATCTTGGTGAATCGCTATCCAAATTGGGGTGATACTGTCGAGATCAATAGCTGGTTC TCCCAGTCCGGGAAAATCGGTATGGGTCGCAATTGGCTAATAAGTGATTGCAACACAGGA GAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCAAAATACGAGAAGATTC TCAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTTGACGCTCCTCCTG TCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTCG 35 CAAGGGTCTAACTCCGGGGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAA GTACATTGGGTGGATTCTCGAGAGTATGCCAACAGAAGTTTTGGAGACCCAGGAGCTATG CTCTCTCACCCTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCCGTGAC CGCTATGAATCCCTCAAAAGTTGGAGACCGGTCTCAGTACCAGCACCTTCTACGGCTTGAG GATGGGGCTGATATCATGAAGGGCAGAACTGAGTGGCGACCAAAGAATGCAGGAACCAA 40 CGGGGCGATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 60 Cuphea heterophylla (Cht) FATB2e (G76D, R97L, H124L, I132S, G152S, H165L, T211N, K258N, C303R, E309G, K334T, T386A variant) coding DNA sequence codon optimized for Prototheca moriformis 45 ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCTCCGGCACCTCCC CCAAGCCCGGCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTC CAACCCCTCCGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGG CTCCGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCC CCCCCCCAGACCTTCCTGAACCAGCTGCCCGACTGGTCCCGCCTGCTGACCGCCATCTCCA 50 CCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAAGTCCAAGCGCCCCG ACATGCTGGTGGACTGGTTCGGCCTGGAGTCCATCGTGCAGGACGGCCTGGTGTTCCGCG AGTCCTACTCCATCCGCTCCTACGAGATCTCCGCCGACCGCACCGCCTCCATCGAGACCGT 67 WO 2014/151904 PCT/US2014/026644 GATGAACCTGCTGCAGGAGACCTCCCTGAACCACTGCAAGTCCATGGGCATCCTGAACGA CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGAT GCAGATCCTGGTGAACCGCTACCCCAACTGGGGCGACACCGTGGAGATCAACTCCTGGTT CTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACTGGCTGATCTCCGACTGCAACACCGG 5 CGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCAGAACACCCGCCGCTT CTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCCCCCCC CGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCAT CCGCAAGGGCCTGACCCCCGGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGT GAAGTACATCGGCTGGATCCTGGAGTCCATGCCCACCGAGGTGCTGGAGACCCAGGAGCT 10 GTGCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGT GACCGCCATGAACCCCTCCAAGGTGGGCGACCGCTCCCAGTACCAGCACCTGCTGCGCCT GGAGGACGGCGCCGACATCATGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA CCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 61 15 Cuphea heterophylla (Cht) FATB2f (R97L, H124L, 1132S, G152S, H165L, T211N variant) amino acid sequence MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSA VSLKSGSLNTQEGTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRPDMLVD WFGLESIVQDGLVFRESYSIRSYEISADRTASIETVMNLLQETSLNHCKSMGILNDGFGRTPEM 20 CKRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWA MMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPEWNDLDV NQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGDRSQYQ HLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS* SEQ ID NO: 62 25 Cuphea heterophylla (Cht) FATB2f (R97L, H124L, I132S, G152S, H165L, T211N variant) coding DNA sequence ATGGTGGTGGCTGCTGCAGCAAGCTCTGCATTCTTCCCTGTTCCGGCATCTGGAACCTCCC CTAAACCCGGGAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGT CAAACCCCAGTGGTGGATTTCAGGTTAAAGCAAATGCCAGTGCTCATCCTAAGGCTAACG 30 GTTCCGCAGTAAGTCTAAAGTCTGGCAGCCTCAACACTCAGGAGGGCACTTCGTCGTCCCC TCCTCCTCGGACATTCCTTAACCAGTTGCCTGATTGGAGTAGGCTTCTGACTGCAATCTCG ACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAAATCTAAGAGGCCT GACATGCTCGTGGACTGGTTTGGGTTGGAGAGTATTGTTCAGGATGGGCTCGTGTTCAGAG AGAGTTATTCGATCAGGTCTTACGAAATAAGCGCTGATCGAACAGCCTCTATAGAGACGG 35 TGATGAACCTCTTGCAGGAAACATCTCTCAATCATTGTAAGAGTATGGGTATTCTCAATGA CGGCTTTGGTCGTACCCCGGAGATGTGCAAAAGGGACCTCATTTGGGTGCTTACAAAAAT GCAGATCTTGGTGAATCGCTATCCAAATTGGGGTGATACTGTCGAGATCAATAGCTGGTTC TCCCAGTCCGGGAAAATCGGTATGGGTCGCAATTGGCTAATAAGTGATTGCAACACAGGA GAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCAAAAGACGAGAAGATTC 40 TCAAAACTTCCAAATGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCCCCTCCTG TCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTTG CAAGGGTCTAACACCGGAGTGGAACGACTTGGATGTCAATCAGCACGTAAGCAACGTGAA GTACATTGGGTGGATTCTCGAGAGTATGCCAAAAGAAGTTTTGGACACCCAGGAGCTATG CTCTCTCACCCTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCTGTGAC 45 CGCTATGGATCCCTCAAAAGTTGGAGACCGATCTCAGTACCAGCACCTTCTGCGGCTTGAA GATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACCAAAGAATGCAGGAACCAA CGGGGCGATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 63 Cuphea heterophylla (Cht) FATB2f (R97L, H124L, I132S, G152S, H165L, T21 1N variant) coding 50 DNA sequence codon optimized for Prototheca moriformis ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCTCCGGCACCTCCC CCAAGCCCGGCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTC 68 WO 2014/151904 PCT/US2014/026644 CAACCCCTCCGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGG CTCCGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCCAGGAGGGCACCTCCTCCTCCCCC CCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGTCCCGCCTGCTGACCGCCATCTCCA CCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAAGTCCAAGCGCCCCG 5 ACATGCTGGTGGACTGGTTCGGCCTGGAGTCCATCGTGCAGGACGGCCTGGTGTTCCGCG AGTCCTACTCCATCCGCTCCTACGAGATCTCCGCCGACCGCACCGCCTCCATCGAGACCGT GATGAACCTGCTGCAGGAGACCTCCCTGAACCACTGCAAGTCCATGGGCATCCTGAACGA CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGAT GCAGATCCTGGTGAACCGCTACCCCAACTGGGGCGACACCGTGGAGATCAACTCCTGGTT 10 CTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACTGGCTGATCTCCGACTGCAACACCGG CGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCAGAAGACCCGCCGCTT CTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCCCCCCC CGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCAT CTGCAAGGGCCTGACCCCCGAGTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGT 15 GAAGTACATCGGCTGGATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCT GTGCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGT GACCGCCATGGACCCCTCCAAGGTGGGCGACCGCTCCCAGTACCAGCACCTGCTGCGCCT GGAGGACGGCACCGACATCATGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA CCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA 20 SEQ ID NO: 64 Cuphea heterophylla (Cht) FATB2g (A6T, A16V, S17P, G76D, R97L, H124L, 1132S, S1431, G152S, A157T, H165L, T21 1N, G414A variant) amino acid sequence MVVAATASSAFFPVPVPGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSA VSLKSGSLNTQEDTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRPDMLVD 25 WFGLESIVQDGLVFREIYSIRSYEISADRTTSIETVMNLLQETSLNHCKSMGILNDGFGRTPEMC KRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWAM MNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPEWNDLDVN QHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGDRSQYQH LLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNANSVS* 30 SEQ ID NO: 65 Cuphea heterophylla (Cht) FATB2g (A6T, A16V, S17P, G76D, R97L, H124L, 1132S, S1431, G152S, A157T, H165L, T21 1N, G414A variant) coding DNA sequence ATGGTGGTGGCTGCTACAGCAAGTTCTGCATTCTTCCCTGTTCCTGTACCTGGAACCTCCC CTAAACCCGGAAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGT 35 CAAACCCCAGTGGTGGATTTCAGGTTAAGGCAAATGCCAGTGCTCATCCTAAGGCTAACG GTTCTGCAGTAAGTCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCC TCCTCCTCGGACATTCCTTAACCAGTTGCCTGATTGGAGTAGGCTTCTGACTGCAATCTCG ACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAAATCTAAGAGGCCT GACATGCTCGTGGACTGGTTTGGGTTGGAGAGTATTGTTCAGGATGGGCTCGTGTTCAGAG 40 AGATTTATTCGATCAGGTCTTACGAAATAAGCGCTGATCGAACAACCTCTATAGAGACGG TGATGAACCTCTTGCAGGAAACATCTCTCAATCATTGTAAGAGTATGGGTATTCTCAATGA CGGCTTTGGTCGTACCCCGGAGATGTGCAAAAGGGACCTCATTTGGGTGCTTACAAAAAT GCAGATCTTGGTGAATCGCTATCCAAATTGGGGTGATACTGTCGAGATCAATAGCTGGTTC TCCCAGTCCGGGAAAATCGGTATGGGTCGCAATTGGCTAATAAGTGATTGCAACACAGGA 45 GAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCAAAAGACGAGAAGATTC TCAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCCCCTCCTG TCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTTG CAAGGGTCTAACACCGGAGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAA GTACATTGGGTGGATTCTCGAGAGTATGCCAAAAGAAGTTTTGGACACCCAGGAGCTATG 50 CTCTCTCACCCTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCTGTGAC CGCTATGGATCCCTCAAAAGTTGGAGACCGATCTCAGTACCAGCACCTTCTGCGGCTTGAA GATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACCAAAGAATGCAGGAACCAA CGGGGCGATATCAACAGGAAAGACTTCAAATGCAAACTCGGTCTCTTAG 69 WO 2014/151904 PCT/US2014/026644 SEQ ID NO: 66 Cuphea heterophylla (Cht) FATB2g (A6T, A16V, S17P, G76D, R97L, H124L, 1132S, S1431, G152S, A157T, H165L, T21 1N, G414A variant) coding DNA sequence codon optimized for Prototheca moriformis 5 ATGGTGGTGGCCGCCACCGCCTCCTCCGCCTTCTTCCCCGTGCCCGTGCCCGGCACCTCCC CCAAGCCCGGCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTC CAACCCCTCCGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGG CTCCGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCC CCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGTCCCGCCTGCTGACCGCCATCTCCA 10 CCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAAGTCCAAGCGCCCCG ACATGCTGGTGGACTGGTTCGGCCTGGAGTCCATCGTGCAGGACGGCCTGGTGTTCCGCG AGATCTACTCCATCCGCTCCTACGAGATCTCCGCCGACCGCACCACCTCCATCGAGACCGT GATGAACCTGCTGCAGGAGACCTCCCTGAACCACTGCAAGTCCATGGGCATCCTGAACGA CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGAT 15 GCAGATCCTGGTGAACCGCTACCCCAACTGGGGCGACACCGTGGAGATCAACTCCTGGTT CTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACTGGCTGATCTCCGACTGCAACACCGG CGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCAGAAGACCCGCCGCTT CTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCCCCCCC CGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCAT 20 CTGCAAGGGCCTGACCCCCGAGTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGT GAAGTACATCGGCTGGATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCT GTGCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGT GACCGCCATGGACCCCTCCAAGGTGGGCGACCGCTCCCAGTACCAGCACCTGCTGCGCCT GGAGGACGGCACCGACATCATGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA 25 CCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGCCAACTCCGTGTCCTGA SEQ ID NO: 67 Cuphea heterophylla (Cht) FATB3aamino acid sequence MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKINGSSVSL KSCSLKTHEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPF 30 GLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNEGFGRTPEMY KRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSV WVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKLPKLDEKTADSIRKGLTPKWNDL DVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKGFGPQFQ HLLRLEDGGEIVKGRTEWRPKTAGINGTIASGETSPGNS* 35 SEQ ID NO: 68 Cuphea heterophylla (Cht) FATB3a coding DNA sequence ATGGTGGCCACCGCTGCAAGTTCTGCATTCTTCCCGGTGCCGTCCCCGGACACCTCCTCTA GACCGGGAAAGCTCGGAAATGGGTCATCAAGCTTGAGGCCCCTCAAGCCCAAATTTGTTG CCAATGCTGGGCTGCAGGTTAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTCCT 40 CGGTCAGTCTAAAGTCTTGCAGTCTCAAGACTCATGAAGACACTCCTTCAGCTCCTCCTCC GCGGACTTTTATCAACCAGTTGCCTGATTGGAGCATGCTTCTTGCTGCAATCACTACTGTC TTCTTGGCAGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCAAAGAGGCCTGACATG CTTGTGGACCCGTTCGGATTGGGAAGGATTGTTCAGGATGGGCTTGTGTTCAGGCAGAATT TTTCGATTAGGTCCTATGAAATAGGCGCTGATCGCACTGCATCCATAGAGACGGTGATGA 45 ACCACTTGCAGGAAACGGCTCTCAATCATGTTAAGAGTGCGGGGCTTCTTAATGAAGGCT TTGGTCGTACTCCTGAGATGTATAAAAGGGACCTTATTTGGGTTGTCGCGAAAATGCAGGT CATGGTTAACCGCTATCCTACTTGGGGTGACACGGTTGAAGTGAATACTTGGGTTGCCAAG TCAGGGAAAAATGGTATGCGTCGTGATTGGCTCATAAGTGATTGCAATACAGGAGAAATT CTTACAAGGGCATCAAGTGTGTGGGTCATGATGAATCAAAAGACAAGAAAATTGTCAAAG 50 ATTCCAGATGAGGTTCGGCATGAGATAGAGCCTCATTTTGTGGACTCTGCTCCCGTCATTG AAGACGATGACTGGAAACTTCCCAAGCTGGATGAGAAAACTGCTGACTCCATCCGCAAGG GTCTAACTCCGAAGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTACA TTGGGTGGATTCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGTTCCCT 70 WO 2014/151904 PCT/US2014/026644 TACCCTGGAATACAGGCGGGAATGCGGAAGGGAGAGTGTGCTGGAGTCCCTCACTGCTGT GGACCCCTCTGGAAAGGGCTTTGGGCCCCAGTTTCAGCACCTTCTGAGGCTTGAGGATGG AGGTGAGATCGTAAAGGGGAGAACTGAGTGGCGACCCAAGACTGCAGGTATCAATGGGA CGATTGCATCTGGGGAGACCTCACCTGGAAACTCTTAG 5 SEQ ID NO: 69 Cuphea heterophylla (Cht) FATB3a coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCCCCGACACCTCCTCCCG CCCCGGCAAGCTGGGCAACGGCTCCTCCTCCCTGCGCCCCCTGAAGCCCAAGTTCGTGGCC 10 AACGCCGGCCTGCAGGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCC GTGTCCCTGAAGTCCTGCTCCCTGAAGACCCACGAGGACACCCCCTCCGCCCCCCCCCCCC GCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGGCCGCCATCACCACCGTGTT CCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAAGCGCCCCGACATGCT GGTGGACCCCTTCGGCCTGGGCCGCATCGTGCAGGACGGCCTGGTGTTCCGCCAGAACTT 15 CTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAA CCACCTGCAGGAGACCGCCCTGAACCACGTGAAGTCCGCCGGCCTGCTGAACGAGGGCTT CGGCCGCACCCCCGAGATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGT GATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCCAA GTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGAT 20 CCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGACCCGCAAGCTGTCCAA GATCCCCGACGAGGTGCGCCACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCCGTGAT CGAGGACGACGACTGGAAGCTGCCCAAGCTGGACGAGAAGACCGCCGACTCCATCCGCA AGGGCCTGACCCCCAAGTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAG TACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGC 25 TCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACC GCCGTGGACCCCTCCGGCAAGGGCTTCGGCCCCCAGTTCCAGCACCTGCTGCGCCTGGAG GACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGACCGCCGGCATCAA CGGCACCATCGCCTCCGGCGAGACCTCCCCCGGCAACTCCTGA SEQ ID NO: 70 30 Cuphea heterophylla (Cht) FATB3b (C67G, H72Q, L128F, N1791 variant) amino acid sequence MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKINGSSVSL KSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPF GFGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLIEGFGRTPEMYK RDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSVW 35 VMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKLPKLDEKTADSIRKGLTPKWNDLD VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKGFGPQFQH LLRLEDGGEIVKGRTEWRPKTAGINGTIASGETSPGNS* SEQ ID NO: 71 Cuphea heterophylla (Cht) FATB3b (C67G, H72Q, L128F, N179I variant) coding DNA sequence 40 ATGGTGGCCACCGCTGCAAGTTCTGCATTCTTCCCGGTGCCATCCCCGGACACCTCCTCTA GACCGGGAAAGCTCGGAAATGGGTCATCAAGCTTGAGGCCCCTCAAGCCCAAATTTGTTG CCAATGCTGGGCTGCAGGTTAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTCCT CGGTCAGTCTAAAGTCTGGCAGTCTCAAGACTCAGGAAGACACTCCTTCGGCTCCTCCTCC GCGGACTTTTATCAACCAGTTGCCTGATTGGAGCATGCTTCTTGCTGCAATCACTACTGTC 45 TTCTTGGCAGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCAAAGAGGCCTGACATG CTTGTGGACCCGTTCGGATTTGGAAGGATTGTTCAGGATGGGCTTGTGTTCAGGCAGAATT TTTCGATTAGGTCCTATGAAATAGGCGCTGATCGCACTGCATCTATAGAGACGGTGATGA ACCACTTGCAGGAAACGGCTCTCAATCATGTTAAGAGTGCGGGGCTTCTTATTGAAGGCTT TGGTCGTACTCCTGAGATGTATAAAAGGGACCTTATTTGGGTTGTCGCGAAAATGCAGGTC 50 ATGGTTAACCGCTATCCTACTTGGGGTGACACGGTTGAAGTGAATACTTGGGTTGCCAAGT CAGGGAAAAATGGTATGCGTCGTGATTGGCTCATAAGTGATTGCAATACAGGAGAAATTC TTACTAGAGCATCAAGTGTGTGGGTCATGATGAATCAAAAGACAAGAAAATTGTCAAAGA 71 WO 2014/151904 PCT/US2014/026644 TTCCAGATGAGGTTCGGCATGAGATAGAGCCTCATTTTGTGGACTCTGCTCCCGTCATTGA AGACGATGACTGGAAACTTCCCAAGCTGGATGAGAAAACTGCTGACTCCATCCGCAAGGG TCTAACTCCGAAGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTACAT TGGGTGGATTCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGTTCCCTT 5 ACCCTGGAATACAGGCGGGAATGCGGAAGGGAGAGTGTGCTGGAGTCCCTCACTGCTGTG GACCCCTCTGGAAAGGGCTTTGGGCCCCAGTTTCAGCACCTTCTGAGGCTTGAGGATGGA GGTGAGATCGTAAAGGGGAGAACTGAGTGGCGACCCAAGACTGCAGGTATCAATGGGAC GATTGCATCTGGGGAGACCTCACCTGGAAACTCTTAG SEQ ID NO: 72 10 Cuphea heterophylla (Cht) FATB3b (C67G, H72Q, L128F, N1791 variant) coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCCCCGACACCTCCTCCCG CCCCGGCAAGCTGGGCAACGGCTCCTCCTCCCTGCGCCCCCTGAAGCCCAAGTTCGTGGCC AACGCCGGCCTGCAGGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCC 15 GTGTCCCTGAAGTCCGGCTCCCTGAAGACCCAGGAGGACACCCCCTCCGCCCCCCCCCCCC GCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGGCCGCCATCACCACCGTGTT CCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAAGCGCCCCGACATGCT GGTGGACCCCTTCGGCTTCGGCCGCATCGTGCAGGACGGCCTGGTGTTCCGCCAGAACTTC TCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAAC 20 CACCTGCAGGAGACCGCCCTGAACCACGTGAAGTCCGCCGGCCTGCTGATCGAGGGCTTC GGCCGCACCCCCGAGATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGTG ATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCCAAG TCCGGCAAGAACGGCATGCGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGATC CTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGACCCGCAAGCTGTCCAAG 25 ATCCCCGACGAGGTGCGCCACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCCGTGATC GAGGACGACGACTGGAAGCTGCCCAAGCTGGACGAGAAGACCGCCGACTCCATCCGCAA GGGCCTGACCCCCAAGTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGT ACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGCT CCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCG 30 CCGTGGACCCCTCCGGCAAGGGCTTCGGCCCCCAGTTCCAGCACCTGCTGCGCCTGGAGG ACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGACCGCCGGCATCAAC GGCACCATCGCCTCCGGCGAGACCTCCCCCGGCAACTCCTGA SEQ ID NO: 73 Cuphea viscosissima (Cvis) FATB1 amino acid sequence 35 MVAAAATSAFFPVPAPGTSPKPGKSGNWPSSLSPTFKPKSIPNGGFQVKANASAHPKANGSAV NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRPDMLVD SVGLKSIVRDGLVSRHSFSIRSYEIGADRTASIETLMNHLQETTINHCKSLGLHNDGFGRTPGM CKNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSQSGKIGMASDWLISDCNTGEILIRATSVW AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDQKLRKFDVKTGDSIRKGLTPRWNDLD 40 VNQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAVDPSENGGRSQYK HLLRLEDGTDIVKSRTEWRPKNAGTNGAISTSTAKTSNGNSVS SEQ ID NO: 74 Cuphea viscosissima (Cvis) FATB 1 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCGCCGCCGCCACCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCTCCCCCA 45 AGCCCGGCAAGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCACCTTCAAGCCCAAGTCCAT CCCCAACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC CGCCGTGAACCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCC CCCCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGACCGCCATCACCACCG TGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCGACA 50 TGCTGGTGGACTCCGTGGGCCTGAAGTCCATCGTGCGCGACGGCCTGGTGTCCCGCCACTC CTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATG AACCACCTGCAGGAGACCACCATCAACCACTGCAAGTCCCTGGGCCTGCACAACGACGGC 72 WO 2014/151904 PCT/US2014/026644 TTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCAG ATCATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACACCTGGTTCTCC CAGTCCGGCAAGATCGGCATGGCCTCCGACTGGCTGATCTCCGACTGCAACACCGGCGAG ATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCTCCC 5 GCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTTCGTGGACTCCCCCCACGTGAT CGAGGACAACGACCAGAAGCTGCGCAAGTTCGACGTGAAGACCGGCGACTCCATCCGCA AGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGT ACATCGGCTGGATCCTGGAGTCCATGCCCATCGAGGTGCTGGAGACCCAGGAGCTGTGCT CCCTGACCGTGGAGTACCGCCGCGAGTGCGGCATGGACTCCGTGCTGGAGTCCGTGACCG 10 CCGTGGACCCCTCCGAGAACGGCGGCCGCTCCCAGTACAAGCACCTGCTGCGCCTGGAGG ACGGCACCGACATCGTGAAGTCCCGCACCGAGTGGCGCCCCAAGAACGCCGGCACCAAC GGCGCCATCTCCACCTCCACCGCCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 75 Cuphea viscosissima (Cvis) FATB2 amino acid sequence 15 MVATAASSAFFPVPSADTSSRPGKLGNGPSSFSPLKPKSIPNGGLQVKASASAPPKINGSSVGLK SGGLKTHDDAPSAPPPRTFINQLPDWSMLLAAITTAFLAAEKQWMMLDRKPKRLDMLEDPFG LGRVVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKTAGLSNDGFGRTPEMYK RDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSVW VMMNQKTRKLSKIPDEVRREIEPHFVDSAPVIEDDDRKLPKLDEKSADSIRKGLTPRWNDLDV 20 NQHVNNAKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGEGYGSQFQHL LRLEDGGEIVKGRTEWRPKNAGINGVVPSEESSPGDYS SEQ ID NO: 76 Cuphea viscosissima (Cvis) FATB2 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCGCCGACACCTCCTCCC 25 GCCCCGGCAAGCTGGGCAACGGCCCCTCCTCCTTCTCCCCCCTGAAGCCCAAGTCCATCCC CAACGGCGGCCTGCAGGTGAAGGCCTCCGCCTCCGCCCCCCCCAAGATCAACGGCTCCTC CGTGGGCCTGAAGTCCGGCGGCCTGAAGACCCACGACGACGCCCCCTCCGCCCCCCCCCC CCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGGCCGCCATCACCACCGCC TTCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACCGCAAGCCCAAGCGCCTGGACATG 30 CTGGAGGACCCCTTCGGCCTGGGCCGCGTGGTGCAGGACGGCCTGGTGTTCCGCCAGAAC TTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGA ACCACCTGCAGGAGACCGCCCTGAACCACGTGAAGACCGCCGGCCTGTCCAACGACGGCT TCGGCCGCACCCCCGAGATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGG TGATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCCA 35 AGTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGA TCCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGACCCGCAAGCTGTCCA AGATCCCCGACGAGGTGCGCCGCGAGATCGAGCCCCACTTCGTGGACTCCGCCCCCGTGA TCGAGGACGACGACCGCAAGCTGCCCAAGCTGGACGAGAAGTCCGCCGACTCCATCCGCA AGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGCCAAGT 40 ACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGCT CCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCG CCGTGGACCCCTCCGGCGAGGGCTACGGCTCCCAGTTCCAGCACCTGCTGCGCCTGGAGG ACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCATCAAC GGCGTGGTGCCCTCCGAGGAGTCCTCCCCCGGCGACTACTCCTGA 45 SEQ ID NO: 77 Cuphea viscosissima (Cvis) FATB3 amino acid sequence MVAAAASSAFFSFPTPGTSPKPGKFGNWPSSLSIPFNPKSNHNGGIQVKANASAHPKANGSAVS LKAGSLETQEDTSSPSPPPRTFISQLPDWSMLVSAITTVFVAAEKQWTMLDRKSKRPDVLVEPF VQDGVSFRQSFSIRSYEIGVDRTASIETLMNIFQETSLNHCKSLGLLNDGFGRTPEMCKRDLIW 50 VVTKMQIEVNRYPTWGDTIEVTTWVSESGKNGMSRDWLISDCHSGEILIRATSVWAMMNQK TRRLSKIPDEVRQEIVPYFVDSAPVIEDDRKLHKLDVKTGDSIRNGLTPRWNDFDVNQHVNNV 73 WO 2014/151904 PCT/US2014/026644 KYIAWLLKSVPTEVFETQELCGLTLEYRRECRRDSVLESVTAMDPSKEGDRSLYQHLLRLENG ADIALGRTEWRPKNAGATGAVSTGKTSNGNSVS SEQ ID NO: 78 Cuphea viscosissima (Cvis) FATB3 coding DNA sequence codon optimized for Prototheca moriformis 5 ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCTCCTTCCCCACCCCCGGCACCTCCCCCAA GCCCGGCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCATCCCCTTCAACCCCAAGTCCAAC CACAACGGCGGCATCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCC GCCGTGTCCCTGAAGGCCGGCTCCCTGGAGACCCAGGAGGACACCTCCTCCCCCTCCCCCC CCCCCCGCACCTTCATCTCCCAGCTGCCCGACTGGTCCATGCTGGTGTCCGCCATCACCAC 10 CGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCGA CGTGCTGGTGGAGCCCTTCGTGCAGGACGGCGTGTCCTTCCGCCAGTCCTTCTCCATCCGC TCCTACGAGATCGGCGTGGACCGCACCGCCTCCATCGAGACCCTGATGAACATCTTCCAG GAGACCTCCCTGAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGGCTTCGGCCGCACC CCCGAGATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGATGCAGATCGAGGTGAAC 15 CGCTACCCCACCTGGGGCGACACCATCGAGGTGACCACCTGGGTGTCCGAGTCCGGCAAG AACGGCATGTCCCGCGACTGGCTGATCTCCGACTGCCACTCCGGCGAGATCCTGATCCGC GCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCCTGTCCAAGATCCCCGAC GAGGTGCGCCAGGAGATCGTGCCCTACTTCGTGGACTCCGCCCCCGTGATCGAGGACGAC CGCAAGCTGCACAAGCTGGACGTGAAGACCGGCGACTCCATCCGCAACGGCCTGACCCCC 20 CGCTGGAACGACTTCGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGCCTGGCTG CTGAAGTCCGTGCCCACCGAGGTGTTCGAGACCCAGGAGCTGTGCGGCCTGACCCTGGAG TACCGCCGCGAGTGCCGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGGACCCCTCC AAGGAGGGCGACCGCTCCCTGTACCAGCACCTGCTGCGCCTGGAGAACGGCGCCGACATC GCCCTGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCGCCACCGGCGCCGTGTCCACC 25 GGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 79 Cuphea calcarata (Ccalc) FATB1 amino acid sequence MVAASASSAFFSVPTPGTSPKPGKFGNWPSSLSVPFKPRSNNSGGFQVKANASAHPKANGSAV SLKSGSLETQEDNSSSSRPPRTFIKQLPDWSMLLSAITTVFVAAEKQWTMFDRKSKRSDMLVD 30 PFVVDRIVQDGVLFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSMGLLYEGFGRTPEMC KRDLIWVVTKIHIKVNRYPTWGDTIEVTTWVSESGKNGMGRDWLISDCHTGEILIRATSVWA MMNQTTRRLSKFPYELRQEIAPHFVDSDPVIEDNRRLLNFDVKTGDSIRKGLTPRWNDLDVNQ HVNNVKYIGWILESVPTEVFDTRELCGLTLEYRQECGRGSVLESVTAMDPSKEGDRSLYQHLL RLEDGTDIVKGRTEWRPKNAGTNGPVSTRKTTNGSSVS 35 SEQ ID NO: 80 Cuphea calcarata (Ccalc) FATB1 coding DNA sequence ATGGTGGCTGCTTCAGCAAGTTCTGCATTCTTCTCCGTCCCAACCCCGGGAACCTCTCCTA AACCCGGGAAGTTCGGCAATTGGCCATCGAGCTTGAGCGTCCCATTCAAGCCCAGATCAA ACAACAGTGGCGGCTTTCAGGTTAAGGCAAACGCCAGTGCTCATCCTAAGGCTAACGGTT 40 CTGCAGTAAGTCTAAAGTCTGGGAGCCTCGAGACTCAGGAGGACAATTCGTCGTCGTCTC GTCCTCCTCGGACTTTCATTAAACAGTTGCCGGACTGGAGTATGCTTCTTTCCGCGATCAC AACCGTCTTCGTGGCGGCTGAGAAGCAGTGGACGATGTTTGATCGGAAATCTAAGAGGTC TGACATGCTCGTGGACCCGTTTGTGGTTGACAGGATTGTTCAGGATGGGGTTCTGTTCAGA CAGAGTTTTTCGATTAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATTGAGACGC 45 TGATGAACATCTTCCAGGAAACATCTCTCAATCATTGTAAGAGTATGGGTCTTCTCTATGA AGGCTTTGGTCGTACTCCTGAGATGTGTAAGAGGGACCTCATTTGGGTGGTTACGAAAAT ACATATCAAGGTGAATCGCTATCCGACTTGGGGTGATACTATCGAGGTCACTACTTGGGTC TCCGAGTCGGGCAAAAACGGTATGGGTCGCGATTGGCTGATAAGTGATTGCCATACAGGA GAAATTCTTATAAGAGCAACGAGTGTGTGGGCTATGATGAATCAAACGACGAGAAGATTG 50 TCGAAATTTCCATATGAGCTTCGACAGGAGATAGCGCCACATTTTGTGGACTCGGATCCTG TCATTGAAGACAATCGAAGATTGCTCAACTTTGATGTGAAGACGGGTGATTCCATTCGCA AGGGTCTAACTCCAAGGTGGAATGACTTGGATGTCAATCAGCACGTTAACAATGTGAAGT 74 WO 2014/151904 PCT/US2014/026644 ACATTGGGTGGATTCTCGAGAGTGTTCCAACAGAAGTTTTCGATACCCGGGAGCTATGCG GCCTCACCCTTGAGTATAGGCAGGAATGCGGAAGAGGAAGTGTGCTGGAGTCCGTGACCG CTATGGATCCCTCAAAAGAGGGAGACCGGTCTCTGTACCAGCACCTTCTTCGGCTTGAGG ATGGGACTGATATCGTGAAGGGCAGAACCGAGTGGCGGCCAAAGAATGCAGGAACCAAT 5 GGGCCAGTATCAACAAGAAAGACTACAAATGGAAGCTCAGTCTCTTAG SEQ ID NO: 81 Cuphea calcarata (Ccalc) FATB 1 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCGCCTCCGCCTCCTCCGCCTTCTTCTCCGTGCCCACCCCCGGCACCTCCCCCA AGCCCGGCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCGTGCCCTTCAAGCCCCGCTCCAA 10 CAACTCCGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC CGCCGTGTCCCTGAAGTCCGGCTCCCTGGAGACCCAGGAGGACAACTCCTCCTCCTCCCGC CCCCCCCGCACCTTCATCAAGCAGCTGCCCGACTGGTCCATGCTGCTGTCCGCCATCACCA CCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGTTCGACCGCAAGTCCAAGCGCTCCG ACATGCTGGTGGACCCCTTCGTGGTGGACCGCATCGTGCAGGACGGCGTGCTGTTCCGCC 15 AGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCT GATGAACATCTTCCAGGAGACCTCCCTGAACCACTGCAAGTCCATGGGCCTGCTGTACGA GGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGAT CCACATCAAGGTGAACCGCTACCCCACCTGGGGCGACACCATCGAGGTGACCACCTGGGT GTCCGAGTCCGGCAAGAACGGCATGGGCCGCGACTGGCTGATCTCCGACTGCCACACCGG 20 CGAGATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGACCACCCGCCGCCT GTCCAAGTTCCCCTACGAGCTGCGCCAGGAGATCGCCCCCCACTTCGTGGACTCCGACCCC GTGATCGAGGACAACCGCCGCCTGCTGAACTTCGACGTGAAGACCGGCGACTCCATCCGC AAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAA GTACATCGGCTGGATCCTGGAGTCCGTGCCCACCGAGGTGTTCGACACCCGCGAGCTGTG 25 CGGCCTGACCCTGGAGTACCGCCAGGAGTGCGGCCGCGGCTCCGTGCTGGAGTCCGTGAC CGCCATGGACCCCTCCAAGGAGGGCGACCGCTCCCTGTACCAGCACCTGCTGCGCCTGGA GGACGGCACCGACATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCACCA ACGGCCCCGTGTCCACCCGCAAGACCACCAACGGCTCCTCCGTGTCCTGA 30 SEQ ID NO: 82 Cuphea painter (Cpai) FATB1 amino acid sequence MVAAAATSAFFPVPAPGTSPNPRKFGSWPSSLSPSLPKSIPNGGFQVKANASAHPKANGSAVSL KSGSLNTQENTSSSPPPRTFLHQLPDWSRLLTAITTVFVKSKRPDMHDRKSKRPDMLVDLFGLE SSVQDALVFRQSF SIRSYEIGTDRTASIETLMNHLQETSLNHCKSTGILLDGFGRTLEMCKRELI 35 WVVIKMQIQVNRYPAWGDTVEINTRFSRLGKIGMGRDWLISDCNTGEILIRATSEYAMMNQK TRRLSKLPYEVHQEIAPLFVDSPPVIEDNDLKVHKFEVKTGDSIQKGLSPGWNDLDVNQHVSN VKYIGWILESMPTEVLETQELCSLALEYRRECGRDSVLESVTAMDPSKVGGRSQYQHLLRLED GTAIVNGITEWRPKNAGANGAISTGKTSNGNSVS 40 SEQ ID NO: 83 Cuphea painter (Cpai) FATB1 coding DNA sequence ATGGTGGCTGCTGCAGCAACTTCTGCATTCTTCCCTGTTCCAGCCCCGGGAACCTCCCCAA ATCCCAGGAAATTCGGAAGTTGGCCATCGAGCTTGAGCCCTTCCTTGCCCAAGTCAATCCC CAATGGCGGATTTCAGGTAAAGGCAAATGCCAGTGCCCATCCGAAGGCTAACGGTTCTGC 45 AGTTAGTCTAAAGTCTGGCAGCCTCAACACTCAGGAGAACACTTCGTCGTCCCCTCCTCCT CGGACTTTCCTTCACCAGTTGCCTGATTGGAGTAGGCTTCTGACTGCAATCACGACCGTGT TCGTGAAATCTAAGAGGCCTGACATGCATGATCGGAAATCTAAGAGGCCTGACATGCTGG TGGACTTGTTTGGGTTGGAAAGTAGTGTTCAGGATGCGCTCGTGTTCAGACAGAGTTTTTC GATTAGGTCTTATGAAATAGGCACTGATCGAACAGCCTCTATAGAGACGCTGATGAACCA 50 CTTGCAGGAAACATCTCTCAATCATTGTAAAAGTACCGGTATTCTCCTTGACGGCTTCGGT CGTACTCTTGAGATGTGTAAAAGGGAACTCATTTGGGTGGTAATAAAAATGCAAATTCAG GTGAATCGCTATCCAGCATGGGGCGATACTGTCGAGATCAATACCCGGTTCTCCCGGTTGG GGAAAATTGGTATGGGTCGCGATTGGCTAATAAGTGATTGCAACACAGGAGAAATTCTAA TAAGAGCAACGAGCGAGTATGCCATGATGAATCAAAAGACGAGAAGACTCTCAAAACTT 75 WO 2014/151904 PCT/US2014/026644 CCATACGAGGTTCACCAGGAGATAGCGCCTCTTTTTGTCGACTCTCCTCCTGTGATTGAAG ACAATGATCTGAAAGTGCATAAATTTGAAGTGAAGACTGGTGATTCCATTCAAAAGGGTC TATCCCCGGGGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTACATTG GGTGGATTCTCGAGAGTATGCCAACAGAAGTTTTGGAGACCCAGGAGCTATGCTCTCTCG 5 CCCTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCCGTGACCGCAATGG ATCCCTCAAAAGTTGGAGGCCGTTCTCAGTACCAGCACCTTCTGCGGCTTGAGGATGGGA CTGCTATCGTGAACGGCATAACTGAGTGGCGGCCGAAGAATGCAGGAGCTAATGGGGCG ATATCAACGGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG 10 SEQ ID NO: 84 Cuphea painter (Cpai) FATB 1 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCGCCGCCGCCACCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCTCCCCCA ACCCCCGCAAGTTCGGCTCCTGGCCCTCCTCCCTGTCCCCCTCCCTGCCCAAGTCCATCCCC AACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCC 15 GTGTCCCTGAAGTCCGGCTCCCTGAACACCCAGGAGAACACCTCCTCCTCCCCCCCCCCCC GCACCTTCCTGCACCAGCTGCCCGACTGGTCCCGCCTGCTGACCGCCATCACCACCGTGTT CGTGAAGTCCAAGCGCCCCGACATGCACGACCGCAAGTCCAAGCGCCCCGACATGCTGGT GGACCTGTTCGGCCTGGAGTCCTCCGTGCAGGACGCCCTGGTGTTCCGCCAGTCCTTCTCC ATCCGCTCCTACGAGATCGGCACCGACCGCACCGCCTCCATCGAGACCCTGATGAACCAC 20 CTGCAGGAGACCTCCCTGAACCACTGCAAGTCCACCGGCATCCTGCTGGACGGCTTCGGC CGCACCCTGGAGATGTGCAAGCGCGAGCTGATCTGGGTGGTGATCAAGATGCAGATCCAG GTGAACCGCTACCCCGCCTGGGGCGACACCGTGGAGATCAACACCCGCTTCTCCCGCCTG GGCAAGATCGGCATGGGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGATCCTG ATCCGCGCCACCTCCGAGTACGCCATGATGAACCAGAAGACCCGCCGCCTGTCCAAGCTG 25 CCCTACGAGGTGCACCAGGAGATCGCCCCCCTGTTCGTGGACTCCCCCCCCGTGATCGAG GACAACGACCTGAAGGTGCACAAGTTCGAGGTGAAGACCGGCGACTCCATCCAGAAGGG CCTGTCCCCCGGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACAT CGGCTGGATCCTGGAGTCCATGCCCACCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCT GGCCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCAT 30 GGACCCCTCCAAGGTGGGCGGCCGCTCCCAGTACCAGCACCTGCTGCGCCTGGAGGACGG CACCGCCATCGTGAACGGCATCACCGAGTGGCGCCCCAAGAACGCCGGCGCCAACGGCGC CATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 85 35 Cuphea hookeriana (Chook) FATB4 amino acid sequence MVAAAATSAFFPVPAPGTSPNPRKFGSWPSSLSPSLPNSIPNGGFQVKANASAHPKANGSAVSL KSGSLNTQENTSSSPPPRTFLHQLPDWSRLLTAITTVFVKSKRPDMHDRKSKRPDMLVDLFGLE SSVQDALVFRQRFSIRSYEIGTDRTASMETLMNHLQETSLNHCKSTGILLDGFGRTLEMCKREL IWVVIKMQIQVNRYPAWGDTVEINTRFSRLGKIGMGRDWLISDCNTGEILIRATSEYAMMNQK 40 TRRLSKLPYEVRQEIAPLFVDSPPVIEDNDLKVHKFEVKTGDSIHKGLTPGWNDLDVNQHVNN VKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAMDPSGGGYGSQFQHLLRLEDG GEIVKGRTEWRPKNGVINGVVPTGESSPGDYS SEQ ID NO: 86 45 Cuphea hookeriana (Chook) FATB4 coding DNA sequence ATGGTGGCTGCTGCAGCAACTTCTGCATTCTTCCCTGTTCCAGCCCCGGGAACCTCCCCTA ATCCCAGGAAATTCGGAAGTTGGCCATCGAGCTTGAGCCCTTCCTTGCCCAACTCAATCCC CAATGGCGGATTTCAGGTAAAGGCAAATGCCAGTGCCCATCCGAAGGCTAACGGTTCTGC AGTTAGTCTAAAGTCTGGCAGCCTCAACACTCAGGAGAACACTTCGTCGTCCCCTCCTCCT 50 CGGACTTTCCTTCACCAGTTGCCTGATTGGAGTAGGCTTCTGACTGCAATCACGACCGTGT TCGTGAAATCTAAGAGGCCTGACATGCATGATCGGAAATCTAAGAGGCCTGACATGCTGG TGGACTTGTTTGGGTTGGAGAGTAGTGTTCAGGATGCGCTCGTGTTCAGACAGAGATTTTC GATTAGGTCTTATGAAATAGGCACTGATCGAACAGCCTCTATGGAGACGCTGATGAACCA CTTGCAGGAAACATCTCTCAATCATTGTAAAAGTACCGGTATTCTCCTTGACGGCTTCGGT 55 CGTACTCTTGAGATGTGTAAAAGGGAACTCATTTGGGTGGTAATAAAAATGCAGATTCAG 76 WO 2014/151904 PCT/US2014/026644 GTGAATCGCTATCCAGCATGGGGCGATACTGTCGAGATCAATACCCGGTTCTCCCGGTTGG GGAAAATTGGTATGGGTCGCGATTGGCTAATAAGTGATTGCAACACAGGAGAAATTCTTA TAAGAGCAACGAGCGAGTATGCCATGATGAATCAAAAGACGAGAAGACTCTCAAAACTT CCATACGAGGTTCGCCAGGAGATAGCGCCTCTTTTTGTCGACTCTCCTCCTGTGATTGAAG 5 ACAATGATCTGAAAGTGCATAAATTTGAAGTGAAGACTGGTGATTCCATTCACAAGGGTC TAACTCCGGGGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTACATCG GGTGGATTCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGTTCCCTTAC TCTGGAATACAGGCGGGAATGTGGAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTATGGA TCCCTCTGGAGGGGGTTATGGGTCCCAGTTTCAGCACCTTCTGCGGCTTGAGGATGGAGGT 10 GAGATCGTGAAGGGGAGAACCGAGTGGCGACCCAAGAATGGTGTAATCAATGGGGTGGT ACCAACCGGGGAGTCCTCACCTGGAGACTACTCTTAG SEQ ID NO: 87 Cuphea hookeriana (Chook) FATB4 coding DNA sequence codon optimized for Prototheca 15 moriformis ATGGTGGCCGCCGCCGCCACCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCTCCCCCA ACCCCCGCAAGTTCGGCTCCTGGCCCTCCTCCCTGTCCCCCTCCCTGCCCAACTCCATCCCC AACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCC GTGTCCCTGAAGTCCGGCTCCCTGAACACCCAGGAGAACACCTCCTCCTCCCCCCCCCCCC 20 GCACCTTCCTGCACCAGCTGCCCGACTGGTCCCGCCTGCTGACCGCCATCACCACCGTGTT CGTGAAGTCCAAGCGCCCCGACATGCACGACCGCAAGTCCAAGCGCCCCGACATGCTGGT GGACCTGTTCGGCCTGGAGTCCTCCGTGCAGGACGCCCTGGTGTTCCGCCAGCGCTTCTCC ATCCGCTCCTACGAGATCGGCACCGACCGCACCGCCTCCATGGAGACCCTGATGAACCAC CTGCAGGAGACCTCCCTGAACCACTGCAAGTCCACCGGCATCCTGCTGGACGGCTTCGGC 25 CGCACCCTGGAGATGTGCAAGCGCGAGCTGATCTGGGTGGTGATCAAGATGCAGATCCAG GTGAACCGCTACCCCGCCTGGGGCGACACCGTGGAGATCAACACCCGCTTCTCCCGCCTG GGCAAGATCGGCATGGGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGATCCTG ATCCGCGCCACCTCCGAGTACGCCATGATGAACCAGAAGACCCGCCGCCTGTCCAAGCTG CCCTACGAGGTGCGCCAGGAGATCGCCCCCCTGTTCGTGGACTCCCCCCCCGTGATCGAG 30 GACAACGACCTGAAGGTGCACAAGTTCGAGGTGAAGACCGGCGACTCCATCCACAAGGG CCTGACCCCCGGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACAT CGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCT GACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCGCCAT GGACCCCTCCGGCGGCGGCTACGGCTCCCAGTTCCAGCACCTGCTGCGCCTGGAGGACGG 35 CGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGGCGTGATCAACGGCG TGGTGCCCACCGGCGAGTCCTCCCCCGGCGACTACTCCTGA SEQ ID NO: 88 Cuphea avigera var. pulcherrima (Ca) FATB1 amino acid sequence 40 MVAAAASSAFFSVPVPGTSPKPGKFRIWPSSLSPSFKPKPIPNGGLQVKANSRAHPKANGSAVS LKSGSLNTQEDTSSSPPPRTFLHQLPDWSRLLTAITTVFVKSKRPDMHDRKSKRPDMLMDSFG LESIVQEGLEFRQSFSIRSYEIGTDRTASIETLMNYLQETSLNHCKSTGILLDGFGRTPEMCKRDL IWVVTKMKIKVNRYPAWGDTVEINTWF SRLGKIGKGRDWLISDCNTGEILIRATSAYATMNQ KTRRLSKLPYEVHQEIAPLFVDSPPVIEDNDLKLHKFEVKTGDSIHKGLTPGWNDLDVNQHVS 45 NVKYIGWILESMPTEVLETQELCSLALEYRRECGRDSVLESVTAMDPTKVGGRSQYQHLLRLE DGTDIVKCRTEWRPKNPGANGAISTGKTSNGNSVS SEQ ID NO: 89 Cuphea avigera var. pulcherrima (Ca) FATB1 coding DNA sequence 50 ATGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCTCTGTTCCAGTCCCGGGAACCTCTCCTA AACCCGGGAAGTTCAGAATTTGGCCATCGAGCTTGAGCCCTTCCTTCAAGCCCAAGCCGA TCCCCAATGGTGGATTGCAGGTTAAGGCAAATTCCAGGGCACATCCGAAGGCTAACGGTT CTGCAGTTAGTCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCCTCC TCCTCGGACTTTCCTTCACCAGTTGCCTGATTGGAGTAGGCTTCTGACTGCAATCACGACC 55 GTGTTCGTGAAATCTAAGAGGCCTGACATGCATGATCGGAAATCTAAGAGGCCTGACATG 77 WO 2014/151904 PCT/US2014/026644 CTGATGGACTCGTTTGGGTTGGAGAGTATTGTTCAAGAAGGGCTCGAGTTCAGACAGAGT TTTTCGATTAGGTCTTATGAAATAGGCACTGATCGAACAGCCTCTATAGAGACGCTGATGA ACTACTTGCAGGAAACATCTCTCAATCATTGTAAGAGTACCGGTATTCTCCTTGACGGCTT TGGTCGTACTCCTGAGATGTGTAAAAGGGACCTCATTTGGGTGGTAACAAAAATGAAGAT 5 CAAGGTGAATCGCTATCCAGCTTGGGGCGATACTGTCGAGATCAATACCTGGTTCTCCCGG TTGGGGAAAATCGGAAAGGGTCGCGATTGGCTAATAAGTGATTGCAACACAGGAGAAATT CTTATAAGAGCAACGAGCGCGTATGCCACGATGAATCAAAAGACGAGAAGACTCTCAAA ACTTCCATACGAGGTTCACCAGGAGATAGCGCCTCTCTTTGTCGACTCTCCTCCTGTCATT GAAGACAATGATCTGAAATTGCATAAGTTTGAAGTGAAGACTGGTGATTCCATTCACAAG 10 GGTCTAACTCCGGGGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTAC ATTGGGTGGATTCTCGAGAGTATGCCAACAGAAGTTTTGGAGACCCAGGAGCTATGCTCT CTCGCCCTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTAGAGTCCGTGACAGCT ATGGATCCCACAAAAGTTGGAGGCCGGTCTCAGTACCAGCACCTTCTGCGACTTGAGGAT GGGACTGATATCGTGAAGTGCAGAACTGAGTGGCGGCCGAAGAATCCAGGAGCTAATGG 15 GGCAATATCAACGGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 90 Cuphea avigera var. pulcherrima (Ca) FATB 1 coding DNA sequence codon optimized for Prototheca moriformis 20 ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCTCCGTGCCCGTGCCCGGCACCTCCCCCA AGCCCGGCAAGTTCCGCATCTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGCCCAAGCCCAT CCCCAACGGCGGCCTGCAGGTGAAGGCCAACTCCCGCGCCCACCCCAAGGCCAACGGCTC CGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCC CCCCGCACCTTCCTGCACCAGCTGCCCGACTGGTCCCGCCTGCTGACCGCCATCACCACCG 25 TGTTCGTGAAGTCCAAGCGCCCCGACATGCACGACCGCAAGTCCAAGCGCCCCGACATGC TGATGGACTCCTTCGGCCTGGAGTCCATCGTGCAGGAGGGCCTGGAGTTCCGCCAGTCCTT CTCCATCCGCTCCTACGAGATCGGCACCGACCGCACCGCCTCCATCGAGACCCTGATGAA CTACCTGCAGGAGACCTCCCTGAACCACTGCAAGTCCACCGGCATCCTGCTGGACGGCTTC GGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGATGAAGATC 30 AAGGTGAACCGCTACCCCGCCTGGGGCGACACCGTGGAGATCAACACCTGGTTCTCCCGC CTGGGCAAGATCGGCAAGGGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGATC CTGATCCGCGCCACCTCCGCCTACGCCACCATGAACCAGAAGACCCGCCGCCTGTCCAAG CTGCCCTACGAGGTGCACCAGGAGATCGCCCCCCTGTTCGTGGACTCCCCCCCCGTGATCG AGGACAACGACCTGAAGCTGCACAAGTTCGAGGTGAAGACCGGCGACTCCATCCACAAG 35 GGCCTGACCCCCGGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTAC ATCGGCTGGATCCTGGAGTCCATGCCCACCGAGGTGCTGGAGACCCAGGAGCTGTGCTCC CTGGCCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCC ATGGACCCCACCAAGGTGGGCGGCCGCTCCCAGTACCAGCACCTGCTGCGCCTGGAGGAC GGCACCGACATCGTGAAGTGCCGCACCGAGTGGCGCCCCAAGAACCCCGGCGCCAACGG 40 CGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCC SEQ ID NO: 91 Cuphea paucipetala (Cpau) FATB1 amino acid sequence MVAAAASSAFFPVPAPGTSPKPGKSGNWPSSLSPSIKPMSIPNGGFQVKANASAHPKANGSAV 45 NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMRDRKSKRPDMLVD SVGLKSVVLDGLVSRQIFSIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMC KNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSHSGKIGMASDWLITDCNTGEILIRATSVWA MMNQKTRRFSRLPYEVRQELTPHYVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLDV NQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAMDPSEDEGRSQYKH 50 LLRLEDGTDIVKGRTEWRPKNAGTNGAISTAKPSNGNSVS SEQ ID NO: 92 Cuphea paucipetala (Cpau) FATB1 coding DNA sequence ATGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGTTCCAGCCCCCGGAACCTCCCCTA 55 AACCCGGGAAGTCCGGCAACTGGCCATCAAGCTTGAGCCCTTCCATCAAGCCCATGTCAA 78 WO 2014/151904 PCT/US2014/026644 TCCCCAATGGCGGATTTCAGGTTAAGGCAAATGCCAGTGCCCATCCTAAGGCTAACGGTT CTGCAGTAAATCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCCTCC TCCTCGGGCTTTCCTTAACCAGTTGCCTGATTGGAGTATGCTTCTGACTGCAATCACGACC GTCTTCGTGGCGGCAGAGAAGCAGTGGACTATGCGTGATCGGAAATCTAAGAGGCCTGAC 5 ATGCTCGTGGACTCGGTTGGGTTGAAGAGTGTTGTTCTGGATGGGCTCGTGTCCAGACAGA TTTTTTCGATTAGGTCTTATGAAATAGGCGCTGATCGAACTGCCTCTATAGAGACGCTGAT GAACCACTTGCAGGAAACATCTATCAATCATTGTAAGAGTTTGGGTCTTCTCAATGACGGC TTTGGTCGTACTCCTGGGATGTGTAAAAATGACCTCATTTGGGTGCTTACAAAAATGCAGA TCATGGTGAATCGCTACCCAACTTGGGGCGATACTGTTGAGATCAATACCTGGTTCTCCCA 10 TTCGGGGAAAATTGGTATGGCTAGCGATTGGCTAATAACTGATTGCAACACAGGAGAAAT TCTTATAAGAGCAACGAGCGTGTGGGCCATGATGAATCAAAAGACGAGAAGATTCTCAAG ACTTCCATACGAGGTTCGCCAGGAGTTAACGCCTCATTATGTGGACTCTCCTCATGTCATT GAAGATAATGATCGGAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTCGTAAG GGTCTAACTCCGAGGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTAC 15 ATTGGGTGGATTCTCGAGAGTATGCCAATAGAAGTTTTGGAGACCCAGGAGCTATGCTCT CTCACCGTTGAATATAGGCGGGAATGCGGAATGGACAGTGTGCTGGAGTCCGTGACTGCT ATGGATCCCTCAGAAGATGAAGGCCGGTCTCAGTACAAGCACCTTCTGCGGCTTGAGGAT GGGACTGACATCGTGAAGGGCAGAACTGAGTGGCGACCGAAGAATGCAGGAACTAACGG GGCGATATCAACAGCAAAGCCTTCAAATGGAAACTCGGTCTCTTAG 20 SEQ ID NO: 93 Cuphea paucipetala (Cpau) FATB 1 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCTCCCCCA AGCCCGGCAAGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCATCAAGCCCATGTCCAT 25 CCCCAACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC CGCCGTGAACCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCC CCCCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGACCGCCATCACCACCG TGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCGCGACCGCAAGTCCAAGCGCCCCGACA TGCTGGTGGACTCCGTGGGCCTGAAGTCCGTGGTGCTGGACGGCCTGGTGTCCCGCCAGA 30 TCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGAT GAACCACCTGCAGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGG CTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCA GATCATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACACCTGGTTCTC CCACTCCGGCAAGATCGGCATGGCCTCCGACTGGCTGATCACCGACTGCAACACCGGCGA 35 GATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCTCC CGCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTACGTGGACTCCCCCCACGTG ATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCCGC AAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAG TACATCGGCTGGATCCTGGAGTCCATGCCCATCGAGGTGCTGGAGACCCAGGAGCTGTGC 40 TCCCTGACCGTGGAGTACCGCCGCGAGTGCGGCATGGACTCCGTGCTGGAGTCCGTGACC GCCATGGACCCCTCCGAGGACGAGGGCCGCTCCCAGTACAAGCACCTGCTGCGCCTGGAG GACGGCACCGACATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCACCAA CGGCGCCATCTCCACCGCCAAGCCCTCCAACGGCAACTCCGTGTCCTGA 45 SEQ ID NO: 94 Cuphea procumbens (Cproc) FATB1 amino acid sequence MVAAAASSAFFPAPAPGSSPKPGKSGNWPSSLSPSFKSKSIPYGRFQVKANASAHPKANGSAV NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVD SVGLKNIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGM 50 CKNDLIWVLTKMQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVW AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLD VNQHVSNVKYIGWILESMPIEVLEAQELCSLTVEYRRECGMDSVLESVTAVDPSEDGGRSQYN HLLRLEDGTDVVKGRTEWRPKNAETNGAISPGNTSNGNSIS 55 79 WO 2014/151904 PCT/US2014/026644 SEQ ID NO: 95 Cuphea procumbens (Cproc) FATB 1 coding DNA sequence ATGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGCTCCAGCCCCGGGATCCTCACCTA AACCCGGGAAGTCCGGTAATTGGCCATCGAGCTTGAGCCCTTCCTTCAAGTCCAAGTCAAT 5 CCCCTATGGCCGATTTCAGGTTAAGGCAAATGCCAGTGCCCATCCTAAGGCTAACGGTTCT GCAGTAAATCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCCTCCTC CTCGGGCTTTCCTTAACCAGTTGCCTGATTGGAGTATGCTTCTGTCTGCAATCACGACTGT ATTCGTGGCGGCAGAGAAGCAGTGGACTATGCTTGATCGGAAATCTAAGAGGCCTGACAT GCTTGTGGACTCGGTTGGGTTGAAGAATATTGTTCGGGATGGGCTCGTGTCCAGACAGAG 10 TTTTTTGATTAGATCTTATGAAATAGGCGCTGATCGAACAGCTTCTATAGAGACACTGATG AACCACTTGCAGGAAACATCTATCAATCATTGTAAGAGTTTGGGTCTTCTCAATGACGGCT TTGGTCGTACTCCTGGGATGTGTAAAAACGACCTCATTTGGGTGCTTACTAAAATGCAGAT CATGGTGAATCGCTACCCAGCTTGGGGCGATACTGTTGAGATCAATACCTGGTTCTCCCAG TCGGGGAAAATCGGTATGGGTAGCGATTGGCTAATAAGTGATTGCAACACAGGAGAAATT 15 CTTATAAGAGCAACGAGCGTGTGGGCCATGATGAATCAAAAAACGAGAAGATTCTCAAG ACTTCCATACGAGGTTCGCCAGGAGTTAACGCCTCATTTTGTGGACTCTCCTCATGTCATT GAAGACAATGATCGGAAATTGCATAAGTTCGATGTGAAGACTGGTGATTCTATTCGCAAG GGTCTAACTCCGAGGTGGAATGACTTGGATGTCAATCAGCACGTGAGCAACGTGAAGTAC ATTGGGTGGATTCTCGAGAGTATGCCAATAGAAGTTTTGGAGGCCCAGGAACTATGCTCT 20 CTCACCGTTGAATATAGGCGGGAATGCGGAATGGACAGTGTGCTGGAGTCCGTGACTGCT GTAGATCCCTCAGAAGATGGAGGCCGGTCTCAGTACAATCACCTTCTGCGGCTTGAGGAT GGGACTGATGTCGTGAAGGGCAGAACTGAGTGGCGACCGAAGAATGCAGAAACTAACGG GGCGATATCACCAGGAAACACTTCAAATGGAAACTCGATCTCCTAG 25 SEQ ID NO: 96 Cuphea procumbens (Cproc) FATB 1 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGCCCCCGCCCCCGGCTCCTCCCCCA AGCCCGGCAAGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGTCCAAGTCCAT 30 CCCCTACGGCCGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC CGCCGTGAACCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCC CCCCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGTCCGCCATCACCACCG TGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCGACA TGCTGGTGGACTCCGTGGGCCTGAAGAACATCGTGCGCGACGGCCTGGTGTCCCGCCAGT 35 CCTTCCTGATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGAT GAACCACCTGCAGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGG CTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCA GATCATGGTGAACCGCTACCCCGCCTGGGGCGACACCGTGGAGATCAACACCTGGTTCTC CCAGTCCGGCAAGATCGGCATGGGCTCCGACTGGCTGATCTCCGACTGCAACACCGGCGA 40 GATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCTCC CGCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTTCGTGGACTCCCCCCACGTG ATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCCGC AAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAG TACATCGGCTGGATCCTGGAGTCCATGCCCATCGAGGTGCTGGAGGCCCAGGAGCTGTGC 45 TCCCTGACCGTGGAGTACCGCCGCGAGTGCGGCATGGACTCCGTGCTGGAGTCCGTGACC GCCGTGGACCCCTCCGAGGACGGCGGCCGCTCCCAGTACAACCACCTGCTGCGCCTGGAG GACGGCACCGACGTGGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGAGACCAA CGGCGCCATCTCCCCCGGCAACACCTCCAACGGCAACTCCATCTCCTGA 50 SEQ ID NO: 97 Cuphea procumbens (Cproc) FATB2 amino acid sequence MVAAAASSAFFPAPAPGSSPKPGKSGNWPSSLSPSFKSKSIPYGRFQVKANASAHPKANGSAV NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVD SVGLKNIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGM 55 CKNDLIWVLTKMQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVW 80 WO 2014/151904 PCT/US2014/026644 AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLD VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRQECGRESVLESLTAVDPSGKGFGSQFQH LLRLEDGGEIVKGRTEWRPKTAGINGAIASGETSPGDF 5 SEQ ID NO: 98 Cuphea procumbens (Cproc) FATB2 coding DNA sequence ATGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGCTCCAGCCCCGGGATCCTCACCTA AACCCGGGAAGTCCGGTAATTGGCCATCGAGCTTGAGCCCTTCCTTCAAGTCCAAGTCAAT CCCCTATGGCCGATTTCAGGTTAAGGCAAATGCCAGTGCCCATCCTAAGGCTAACGGTTCT 10 GCAGTAAATCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCCTCCTC CTCGGGCTTTCCTTAACCAGTTGCCTGATTGGAGTATGCTTCTGTCTGCAATCACGACTGT ATTCGTGGCGGCAGAGAAGCAGTGGACTATGCTTGATCGGAAATCTAAGAGGCCTGACAT GCTTGTGGACTCGGTTGGGTTGAAGAATATTGTTCGGGATGGGCTCGTGTCCAGACAGAG TTTTTTGATTAGATCTTATGAAATAGGCGCTGATCGAACAGCTTCTATAGAGACACTGATG 15 AACCACTTGCAGGAAACATCTATCAATCATTGTAAGAGTTTGGGTCTTCTCAATGACGGCT TTGGTCGTACTCCTGGGATGTGTAAAAACGACCTCATTTGGGTGCTTACTAAAATGCAGAT CATGGTGAATCGCTACCCAGCTTGGGGCGATACTGTTGAGATCAATACCTGGTTCTCCCAG TCGGGGAAAATCGGTATGGGTAGCGATTGGCTAATAAGTGATTGCAACACAGGAGAAATT CTTATAAGAGCAACGAGCGTGTGGGCCATGATGAATCAAAAAACGAGAAGATTCTCAAG 20 ACTTCCATACGAGGTTCGCCAGGAGTTAACGCCTCATTTTGTGGACTCTCCTCATGTCATT GAAGACAATGATCGGAAATTGCATAAGTTCGATGTGAAGACTGGTGATTCTATTCGCAAG GGTCTAACTCCGAGGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTAC ATCGGGTGGATTCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGTTCCC TTACCCTGGAATACAGGCAGGAATGCGGAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTG 25 TGGACCCCTCTGGAAAGGGCTTTGGGTCCCAGTTCCAACACCTTCTGAGGCTTGAGGATGG AGGTGAGATCGTGAAGGGGAGAACTGAGTGGCGACCCAAGACTGCAGGTATCAATGGGG CGATAGCATCCGGGGAGACCTCACCTGGAGACTTTTAG SEQ ID NO: 99 30 Cuphea procumbens (Cproc) FATB2 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGCCCCCGCCCCCGGCTCCTCCCCCA AGCCCGGCAAGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGTCCAAGTCCAT CCCCTACGGCCGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC 35 CGCCGTGAACCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCC CCCCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGTCCGCCATCACCACCG TGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCGACA TGCTGGTGGACTCCGTGGGCCTGAAGAACATCGTGCGCGACGGCCTGGTGTCCCGCCAGT CCTTCCTGATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGAT 40 GAACCACCTGCAGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGG CTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCA GATCATGGTGAACCGCTACCCCGCCTGGGGCGACACCGTGGAGATCAACACCTGGTTCTC CCAGTCCGGCAAGATCGGCATGGGCTCCGACTGGCTGATCTCCGACTGCAACACCGGCGA GATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCTCC 45 CGCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTTCGTGGACTCCCCCCACGTG ATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCCGC AAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAA GTACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTG CTCCCTGACCCTGGAGTACCGCCAGGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGAC 50 CGCCGTGGACCCCTCCGGCAAGGGCTTCGGCTCCCAGTTCCAGCACCTGCTGCGCCTGGA GGACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGACCGCCGGCATCA ACGGCGCCATCGCCTCCGGCGAGACCTCCCCCGGCGACTTCTGA SEQ ID NO: 100 55 Cupheaprocumbens (Cproc) FATB3 amino acid sequence 81 WO 2014/151904 PCT/US2014/026644 MVAAAASSAFFPAPAPGSSPKPGKSGNWPSSLSPSFKSKSIPYGRFQVKANASAHPKANGSAV NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVD SVGLKNIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGM CKNDLIWVLTKMQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVW 5 AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLD VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGEGGYGSQFQ HLLRLEDGGEIVKGRTEWRPKNAGINGVLPTGE* SEQ ID NO: 101 10 Cuphea procumbens (Cproc) FATB3 coding DNA sequence ATGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGCTCCAGCCCCGGGATCCTCACCTA AACCCGGGAAGTCCGGTAATTGGCCATCGAGCTTGAGCCCTTCCTTCAAGTCCAAGTCAAT CCCCTATGGCCGATTTCAGGTTAAGGCAAATGCCAGTGCCCATCCTAAGGCTAACGGTTCT GCAGTAAATCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCCTCCTC 15 CTCGGGCTTTCCTTAACCAGTTGCCTGATTGGAGTATGCTTCTGTCTGCAATCACGACTGT ATTCGTGGCGGCAGAGAAGCAGTGGACTATGCTTGATCGGAAATCTAAGAGGCCTGACAT GCTTGTGGACTCGGTTGGGTTGAAGAATATTGTTCGGGATGGGCTCGTGTCCAGACAGAG TTTTTTGATTAGATCTTATGAAATAGGCGCTGATCGAACAGCTTCTATAGAGACACTGATG AACCACTTGCAGGAAACATCTATCAATCATTGTAAGAGTTTGGGTCTTCTCAATGACGGCT 20 TTGGTCGTACTCCTGGGATGTGTAAAAACGACCTCATTTGGGTGCTTACTAAAATGCAGAT CATGGTGAATCGCTACCCAGCTTGGGGCGATACTGTTGAGATCAATACCTGGTTCTCCCAG TCGGGGAAAATCGGTATGGGTAGCGATTGGCTAATAAGTGATTGCAACACAGGAGAAATT CTTATAAGAGCAACGAGCGTGTGGGCCATGATGAATCAAAAAACGAGAAGATTCTCAAG ACTTCCATACGAGGTTCGCCAGGAGTTAACGCCTCATTTTGTGGACTCTCCTCATGTCATT 25 GAAGACAATGATCGGAAATTGCATAAGTTCGATGTGAAGACTGGTGATTCTATTCGCAAG GGTCTAACTCCGAGGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTAC ATCGGGTGGATTCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGTTCCC TTACCCTGGAATACAGGCGGGAATGTGGAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTG TGGACCCCTCTGGAGAGGGGGGCTATGGATCCCAGTTTCAGCACCTTCTGCGGCTTGAGG 30 ATGGAGGTGAGATCGTGAAGGGGAGAACTGAGTGGCGACCCAAGAATGCTGGAATCAAT GGGGTGTTACCAACCGGGGAGTAG SEQ ID NO: 102 Cuphea procumbens (Cproc) FATB3 coding DNA sequence codon optimized for Prototheca 35 moriformis ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGCCCCCGCCCCCGGCTCCTCCCCCA AGCCCGGCAAGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGTCCAAGTCCAT CCCCTACGGCCGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC CGCCGTGAACCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCC 40 CCCCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGTCCGCCATCACCACCG TGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCGACA TGCTGGTGGACTCCGTGGGCCTGAAGAACATCGTGCGCGACGGCCTGGTGTCCCGCCAGT CCTTCCTGATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGAT GAACCACCTGCAGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGG 45 CTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCA GATCATGGTGAACCGCTACCCCGCCTGGGGCGACACCGTGGAGATCAACACCTGGTTCTC CCAGTCCGGCAAGATCGGCATGGGCTCCGACTGGCTGATCTCCGACTGCAACACCGGCGA GATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCTCC CGCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTTCGTGGACTCCCCCCACGTG 50 ATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCCGC AAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAA GTACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTG CTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGAC CGCCGTGGACCCCTCCGGCGAGGGCGGCTACGGCTCCCAGTTCCAGCACCTGCTGCGCCT 55 GGAGGACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA TCAACGGCGTGCTGCCCACCGGCGAGTGA 82 WO 2014/151904 PCT/US2014/026644 SEQ ID NO: 103 Cuphea ignea (Cignea) FATB1 amino acid sequence PGTSRKTGKFGNWPSSLSPSFKPKSIPNGGFQVKANARAHPKANGSAVSLKSVSLNTQEDTSLS PPPRAFLNQLPDWRMLRTALTTVFVAAEKQWTMLDRKSKRPDMLVDSFGLESIVQEGLVFRQ 5 SFSIRSYEIGIDRTASIETLMNHLQETSLNQCKSAGILHDGFGRTLEMCKRDLIWVVTKMQIKV NRYPAWGDTVEISTRFSRLGKIGMGRDWLICDCNTGEILIRATSAYAMMNQKTRRLSKLPNEV RQEIAPLFVDSDPVIEENDMKLHKFEVKTGDSICKGLTPRWSDLDVNQHVSNVKYIGWILESM PTEVLETQELCSLALEYRRECGRDSVLESVTSMDPSKVGGWSQYQHLLRLEDGADIVKGRTE WRPKNAGANGAISTGKT 10 SEQ ID NO: 104 Cuphea ignea (Cignea) FATB1 coding DNA sequence CCGGGAACCTCACGTAAAACCGGGAAGTTCGGCAATTGGCCATCAAGCTTGAGCCCTTCC TTCAAGCCCAAGTCAATCCCCAATGGCGGATTTCAGGTTAAGGCTAATGCCAGAGCCCAT 15 CCTAAGGCTAACGGTTCTGCAGTAAGTCTAAAGTCTGTCAGCCTCAACACTCAGGAGGAC ACTTCGTTGTCCCCTCCTCCTCGTGCTTTCCTTAACCAGTTGCCTGATTGGAGGATGCTTCG GACTGCACTCACGACCGTCTTTGTGGCGGCAGAGAAGCAGTGGACTATGCTTGATCGGAA ATCTAAGAGGCCTGACATGCTCGTGGACTCGTTTGGGTTGGAGAGTATTGTTCAAGAAGG GCTCGTGTTCAGACAGAGCTTTTCGATTAGGTCTTATGAAATAGGCATTGATCGAACAGCC 20 TCTATAGAGACGCTGATGAACCACTTGCAGGAAACATCTCTCAATCAATGTAAGAGTGCT GGTATTCTCCATGACGGCTTCGGTCGTACTCTTGAGATGTGTAAAAGGGACCTCATTTGGG TTGTTACGAAAATGCAGATCAAGGTGAATCGCTATCCAGCTTGGGGCGATACTGTCGAGA TCAGTACCCGGTTCTCCCGGTTGGGGAAAATCGGTATGGGTCGCGATTGGCTAATATGTGA TTGCAACACAGGAGAAATTCTTATAAGAGCAACGAGCGCGTATGCCATGATGAATCAAAA 25 GACGAGAAGACTCTCAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCTTTTTGT GGACTCTGATCCTGTCATTGAAGAAAATGATATGAAATTGCATAAGTTTGAAGTGAAGAC TGGTGATTCCATTTGCAAGGGTCTAACTCCGAGGTGGAGTGACTTGGATGTCAATCAGCAC GTAAGCAACGTGAAGTACATAGGGTGGATTCTCGAGAGTATGCCAACAGAAGTTTTGGAG ACACAGGAGCTATGCTCTCTCGCCCTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTG 30 CTGGAGTCTGTGACCTCTATGGATCCCTCAAAAGTTGGAGGCTGGTCTCAGTACCAGCACC TTCTGCGACTTGAGGATGGGGCGGATATCGTGAAGGGCAGAACTGAGTGGCGGCCGAAG AATGCAGGAGCTAACGGGGCGATATCAACAGGAAAGACTTGA SEQ ID NO: 105 35 Cuphea ignea (Cignea) FATB1 coding DNA sequence codon optimized for Prototheca moriformis CCCGGCACCTCCCGCAAGACCGGCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCT TCAAGCCCAAGTCCATCCCCAACGGCGGCTTCCAGGTGAAGGCCAACGCCCGCGCCCACC CCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGTCCGTGTCCCTGAACACCCAGGAGGACA CCTCCCTGTCCCCCCCCCCCCGCGCCTTCCTGAACCAGCTGCCCGACTGGCGCATGCTGCG 40 CACCGCCCTGACCACCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAA GTCCAAGCGCCCCGACATGCTGGTGGACTCCTTCGGCCTGGAGTCCATCGTGCAGGAGGG CCTGGTGTTCCGCCAGTCCTTCTCCATCCGCTCCTACGAGATCGGCATCGACCGCACCGCC TCCATCGAGACCCTGATGAACCACCTGCAGGAGACCTCCCTGAACCAGTGCAAGTCCGCC GGCATCCTGCACGACGGCTTCGGCCGCACCCTGGAGATGTGCAAGCGCGACCTGATCTGG 45 GTGGTGACCAAGATGCAGATCAAGGTGAACCGCTACCCCGCCTGGGGCGACACCGTGGAG ATCTCCACCCGCTTCTCCCGCCTGGGCAAGATCGGCATGGGCCGCGACTGGCTGATCTGCG ACTGCAACACCGGCGAGATCCTGATCCGCGCCACCTCCGCCTACGCCATGATGAACCAGA AGACCCGCCGCCTGTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCTGTTCG TGGACTCCGACCCCGTGATCGAGGAGAACGACATGAAGCTGCACAAGTTCGAGGTGAAG 50 ACCGGCGACTCCATCTGCAAGGGCCTGACCCCCCGCTGGTCCGACCTGGACGTGAACCAG CACGTGTCCAACGTGAAGTACATCGGCTGGATCCTGGAGTCCATGCCCACCGAGGTGCTG GAGACCCAGGAGCTGTGCTCCCTGGCCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCC GTGCTGGAGTCCGTGACCTCCATGGACCCCTCCAAGGTGGGCGGCTGGTCCCAGTACCAG CACCTGCTGCGCCTGGAGGACGGCGCCGACATCGTGAAGGGCCGCACCGAGTGGCGCCCC 55 AAGAACGCCGGCGCCAACGGCGCCATCTCCACCGGCAAGACCTGA 83 WO 2014/151904 PCT/US2014/026644 SEQ ID NO: 106 JcFatB1 consensus amino acid sequence MVAAAASSAFFPVPAPGTSPKPGKSGNWPSSLSPSFKPKSIPNGGFQVKANASAHPKANGSAV NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRPDMLVD 5 SVGLKRIVQDGLVSRQSFSIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGM CKNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVW AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLD VNQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAMDPSENGGRSQYK HLLRLEDGTDIVKGRTEWRPKNAGTNGAISTGKTSNGNSVS* 10 SEQ ID NO: 107 JcFatBl consensus DNA sequence codon optimized for Prototheca ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCTCCCCCA AGCCCGGCAAGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGCCCAAGTCCAT 15 CCCCAACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC CGCCGTGAACCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCC CCCCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGACCGCCATCACCACCG TGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCGACA TGCTGGTGGACTCCGTGGGCCTGAAGCGCATCGTGCAGGACGGCCTGGTGTCCCGCCAGT 20 CCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGAT GAACCACCTGCAGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGG CTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCA GATCATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACACCTGGTTCTC CCAGTCCGGCAAGATCGGCATGGGCTCCGACTGGCTGATCTCCGACTGCAACACCGGCGA 25 GATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCTCC CGCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTTCGTGGACTCCCCCCACGTG ATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCCGC AAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAG TACATCGGCTGGATCCTGGAGTCCATGCCCATCGAGGTGCTGGAGACCCAGGAGCTGTGC 30 TCCCTGACCGTGGAGTACCGCCGCGAGTGCGGCATGGACTCCGTGCTGGAGTCCGTGACC GCCATGGACCCCTCCGAGAACGGCGGCCGCTCCCAGTACAAGCACCTGCTGCGCCTGGAG GACGGCACCGACATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCACCAA CGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA 35 SEQ ID NO: 108 JcFatB2 consensus amino acid sequence MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLSPLKPKSVANGGLQVKANASAPPKINGSSVGL KSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPF GLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFGRTPEMY 40 KRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSV WVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTPKWNDLD VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKGYGSQFQ HLLRLEDGGEIVKGRTEWRPKTAGINGAIASGETSPGDSS* 45 SEQ ID NO: 109 JcFatB2 consensus DNA sequence codon optimized for Prototheca ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCCCCGACACCTCCTCCCG CCCCGGCAAGCTGGGCAACGGCTCCTCCTCCCTGTCCCCCCTGAAGCCCAAGTCCGTGGCC AACGGCGGCCTGCAGGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCC 50 GTGGGCCTGAAGTCCGGCTCCCTGAAGACCCAGGAGGACACCCCCTCCGCCCCCCCCCCC CGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGGCCGCCATCACCACCGTGT TCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAAGCGCCCCGACATGC TGGTGGACCCCTTCGGCCTGGGCCGCATCGTGCAGGACGGCCTGGTGTTCCGCCAGAACTT 84 WO 2014/151904 PCT/US2014/026644 CTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAA CCACCTGCAGGAGACCGCCCTGAACCACGTGAAGTCCGCCGGCCTGCTGAACGACGGCTT CGGCCGCACCCCCGAGATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGT GATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCCAA 5 GTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGAT CCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGACCCGCCGCCTGTCCAA GATCCCCGACGAGGTGCGCCACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCCGTGAT CGAGGACGACGACCGCAAGCTGCCCAAGCTGGACGAGAAGACCGCCGACTCCATCCGCA AGGGCCTGACCCCCAAGTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAG 10 TACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGC TCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACC GCCGTGGACCCCTCCGGCAAGGGCTACGGCTCCCAGTTCCAGCACCTGCTGCGCCTGGAG GACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGACCGCCGGCATCAA CGGCGCCATCGCCTCCGGCGAGACCTCCCCCGGCGACTCCTCCTGA 15 SEQ ID NO: 110 CuPSR23 FATB3 amino acid sequence MVVAAATSAFFPVPAPGTSPKPGKSGNWPSSLSPTFKPKSIPNAGFQVKANASAHPKA NGSAVNLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRP 20 DMLVDSVGLKCIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFG RTPGMCKNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSQSGKIGMASDWLISDCNTGEILIR ATSVWAMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDQKLHKFDVKTGDSIRKGLTPR WNDLDVNQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAVDPSENG GRSQYKHLLRLEDGTDIVKSRTEWRPKNAGTNGAISTSTAKTSNGNSVS 25 SEQIDNO: 111 CuPSR23 FATB3b amino acid sequence MVVAAATSAFFPVPAPGTSPKPGKSGNWPSSLSPTFKPKSIPNAGFQVKANASAHPKA NGSAVNLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRP DMLVDSVGLKSIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFG 30 RTPGMCKNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSQSGKIGMASDWLISDCNTGEILIR ATSVWAMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDQKLHKFDVKTGDSIRKGLTPR WNDLDVNQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAVDPSENG GRSQYKHLLRLEDGTDIVKSRTEWRPKNAGTNGAISTSTAKTSNGNSAS SEQ ID NO: 112 35 CwFATB3 amino acid sequence: MVVAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSPHPK ANGSAVSLKSGSLNTLEDPPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQFTRLDRKSKRPD MLVDWFGSETIVQDGLVFRERFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFG RTSEMCTRDLIWVLTKMQIVVNRYPTWGDTVEINSWFSQSGKIGMGRDWLISDCNTGEILVR 40 ATSAWAMMNQKTRRFSKLPCEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPG WNDLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRRECGRESVVESVTSMNPSKVG DRSQYQHLLRLEDGADIMKGRTEWRPKNAGTNRAIST SEQ ID NO: 113 CwFATB3a amino acid sequence: 45 MVVAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSPHPK ANGSAVSLKSGSLNTLEDPPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQFTRLDRKSKRPD MLVDWFGSETIVQDGLVFRERFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFG RTSEMCTRDLIWVLTKMQIVVNRYPTWGDTVEINSWFSQSGKIGMGRDWLISDCNTGEILVR ATSAWAMMNQKTRRFSKLPCEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPG 50 WNDLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRRECGRESVVESVTSMNPSKVG DRSQYQHLLRLEDGADIMKGRTEWRPKNAGTNRAIST 85 WO 2014/151904 PCT/US2014/026644 SEQ ID NO: 114 CwFATB3b amino acid sequence MVVAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSPHPK ANGSAVSLKSGSLNTLEDLPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQFTRLDRKSKRPD 5 MLVDWFGSETIVQDGLVFRERFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFG RTSEMCTRDLIWVLTKMQIVVNRYPTWGDTVEINSWFSQSGKIGMGRDWLISDCNTGEILVR ATSAWAMMNQKTRRFSKLPCEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPG WNDLDVNQHVSNVKYIGWILEKFWRPRSYALSPLNIGGNVEGKVW SEQ ID NO: 115 10 CwFATB3c amino acid sequence MVVAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSPHPK ANGSAVSLKSGSLNTLEDLPSSPPPRTFLNQLPDWSRLRTAITTVFVATEKQFTRLDRKSKRPD MLVDWFGSETIVQDGLVFRERFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFG RTSEMCTRDLIWVLTKMQIVVNRYPTWGDTVEINSWFSQSGKIGMGRDWLISDCNTGEILVR 15 ATSAWAMMNQKTRRFSKLPCEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPG WNDLDVNQHVSNVKYIGWILEKFWRPRSYALSPLNIGGNVEGKVW SEQ ID NO: 116 CwFATB4a amino acid sequence MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPPKIN 20 GSSVGLKSGGFKTQEDSPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSNDGFGR TPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILT RASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPVVEDDDRKLPKLDENTADSIRKGLTPR WNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSAEGY 25 ASRFQHLLRLEDGGEIVKARTEWRPKNAGINGVVPSEESSPGDFF SEQ ID NO: 117 CwFATB4a.1 amino acid sequence MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPPKIN GSSVGLKSGGFKTQEDSPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD 30 MLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSNDGFGR TPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILT RASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPVVEDDDRKLPKLDENTADSIRKGLTPR WNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSAEGY ASRFQHLLRLEDGGEIVKARTEWRPKNAGINWVVPSEESSPGDFF 35 SEQ ID NO: 118 CwFATB4a.2 amino acid sequence: MVATAASSAFFPVPSADTSSSRPGKLGNGPSSLSPLKPKSIPNGGLQVKANASAPPKIN GSSVGLKSGSFKTQEDAPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSNDGFGR 40 TPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILT RASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPVVEDDDRKLPKLDENTADSIRKGLTPR WNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSAEGY ASRFQHLLRLEDGGEIVKARTEWRPKNAGINGVVPSEESSPGDFF SEQ ID NO: 119 45 CwFATB4a.3 amino acid sequence MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPPKIN GSSVGLKSGGFKTQEDSPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSNDGFGR TPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILT 50 RASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPVVEDDDRKLPKLDENTADSIRKGLTPR 86 WO 2014/151904 PCT/US2014/026644 WNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSAEGY VSRFQHLLRLEDGGEIVKARTEWRPKNAGINGVVPSEESSPGDFF SEQ ID NO: 120 CwFATB4b amino acid sequence 5 MVATAASSAFFPVPSADTSSSRPGKLGNGPSSLSPLKPKSIPNGGLQVKANASAPPKIN GSSVGLKSGSFKTQEDAPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSSDGFGR TPAMSKRDLIWVVAKMQVMVNRYPAWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILT RASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPVVEDDDRKLPKLDENTADSIRKGLTPR 10 WNDLDVNQHVNNVKYIGWILESTPAEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGEGD GSKFQHLLRLEDGGEIVKARTEWRPKNAGINGVVPSEESSPGGDFF SEQ ID NO: 121 CwFATB4b. 1 amino acid sequence MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPPKIN 15 GSSVGLKSGSFKTQEDAPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSSDGFGR TPAMSKRDLIWVVAKMQVMVNRYPAWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILT RASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPVVEDDDRKLPKLDENTADSIRKGLTPR WNDLDVNQHVNNVKYIGWILESTPAEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGEGD 20 GSKFQHLLRLEDGGEIVKARTEWRPKNAGINGVVPSEESSPGGDFF SEQ ID NO: 122 CwFATB5 amino acid sequence MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPKANG SAVNLKSGSLETPPRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRIFQDGVFF 25 RQSFSIRSYEIGVDRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWVVTKIQVE VNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNQNTRRLSKFP YEVRQEIAPHFVDSAPVIEDDQKLQKLDVKTGDSIRDGLTPRWNDLDVNQHVNNVKYIGWIL KSVPIEVFETQELCGVTLEYRRECGRDSVLESVTAMDPAKEGDRCVYQHLLRLEDGADITIGR TEWRPKNAGANGAMSSGKTSNGNCLIEGRGWQPFRVVRLIF 30 SEQ ID NO: 123 CwFATB5a amino acid sequence MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPKANG SAVNLKSGSLETPPRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRIFQDGFFFR QSFSIRSYEIGVDRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWVVTKIQVEV 35 NRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNQNTRRLSKFPYE VRQEIAPHFVDSAPVIEDDQKLQKLDVKTGDSIRDGLTPRWNDLDVNQHVNNVKYIGWILKS VPIEVFETQELCGVTLEYRRECGRDSVLESVTAMDPAKEGDRCVYQHLLRLEDGADITIGRTE WRPKNAGANGAMSSGKTSNGNCLIEGRGWQPFRVVRLIF SEQ ID NO: 124 40 CwFATB5b amino acid sequence MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPKANG SAVNLKSGSLETPPRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRIFQDGVFF RQSFSIRSYEIGVDRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWVVTKIQVE VNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNQNTRRLSKFP 45 YEVRQEIAPHFVDSAPVIEDDQKLQKLDVKTGDSIRDGLTPRWNDLDVNQHVNNVKYIGWIL KSVPIEVFETQELCGVTLEYRRECGRDSVLESVTAMDPAKEGDRCVYQHLLWLEDGADITIGR TEWRPKNAGANGAMSSGKTSNGNCLIEGRGWQPFRVVRLIF SEQ ID NO: 125 CwFATB5c amino acid sequence 50 MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPKANG 87 WO 2014/151904 PCT/US2014/026644 SAVNLKSGSLETPPRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRIFQDGVFF RQSFSIRSYEIGVDRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWVVTKIQVE VNRYPIWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNQNTRRLSKFPY EVRQEIAPHFVDSAPVIEDDQKLQKLDVKTGDSIRDGLTPRWNDLDVNQHVNNVKYIGWILK 5 SVPIEVFETQELCGVTLEYRRECGRDSVLESVTAMDPAKEGDRCVYQHLLRLEDGADITIGRTE WRPKNAGANGAMSSGKTSNGNCLIEGMGWQPFRVVRLIF SEQ ID NO: 126 CwFATB5.1 amino acid sequence MVAAAASSAFFSVPTPGTSPKPGKFRNWPSSLSVPFKPETNHNGGFHIKANASAHPKA 10 NGSALNLKSGSLETQEDTSLSSPPRTFIKQLPDWSMLLSKITTVFGAAEKQLKRPGMLVEPFGV DRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLI WVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNQ NTRRLSKFPYEVRQEIAPHFVDSAPVIEDDRKLYKLNVKTGDSIRDGLTPRWNDLDVNQHVNN VKFIGWILKSVPTKVFETQELCGVTLEYRRECGKDSVLESVTAMDPAKEGDRSVYQHLLRLED 15 GADITIGRTEWRPKNAGANEAISSGKTSNGNSAS SEQ ID NO: 127 CwFATB5. 1 a amino acid sequence MVAAAASSAFFSVPTPGTSPKPGKFRNWPLSLSVPFKPETNHNGGFHIKANASAHPKA NGSALNLKSGSLETQEDTSLSSPPRTFIKQLPDWSMLLSKITTVFGAAEKQLKRPGMLVEPFGV 20 DRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLI WVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNQ NTRRLSKFPYEVRQEIAPHFVDSAPVIEDDRKLYKLNVKTGDSIRDGLTPRWNDLDVNQHVNN VKFIGWILKSVPTKVFETQELCGVTLEYRRECGKDSVLESVTAMDPAKEGDRSVYQHLLRLED GADITIGRTEWRPKNAGANEAISSGKTSNGNSAS 25 SEQ ID NO: 128 CcFATB2b amino acid sequence MVTTSLASAYFSMKAVMLAPDGRGIKPRSSGLQVRAGNERNSCKVINGTKVKDTEG LKGCSTLQGQSMLDDHFGLHGLVFRRTFAIRCYEVGPDRSTSIMAVMNHLQEAARNHAESLG LLGDGFGETLEMSKRDLIWVVRRTHVAVERYPAWGDTVEVEAWVGASGNTGMRRDFLVRD 30 CKTGHILTRCTSVSVMMNMRTRRLSKIPQEVRAEIDPLFIEKVAVKEGEIKKLQKLNDSTADYI QGGWTPRWNDLDVNQHVNNIIYVGWIFKSVPDSISENHHLSSITLEYRRECIRGNKLQSLTTVC GGSSEAGIICEHLLQLEDGSEVLRARTEWRPKHTDSFQGISERFPQQEPHK SEQ ID NO: 129 CcFATB3 amino acid sequence 35 MVATAAASAFFPVGAPATSSATSAKASMMPDNLDARGIKPKPASSSGLQVKANAHA SPKINGSKVSTDTLKGEDTLTSSPAPRTFINQLPDWSMFLAAITTIFLAAEKQWTNLDWKPRRP DMLADPFGIGRFMQDGLIFRQHFAIRSYEIGADRTASIETLMNHLQETALNHVRSAGLLGDGF GATPEMSRRDLIWVVTRMQVLVDRYPAWGDIVEVETWVGASGKNGMRRDWLVRDSQTGEI LTRATSVWVMMNKRTRRLSKLPEEVRGEIGPYFIEDVAIIEEDNRKLQKLNENTADNVRRGLT 40 PRWSDLDVNQHVNNVKYIGWILESAPGSILESHELSCMTLEYRRECGKDSVLQSMTAVSGGG SAAGGSPESSVECDHLLQLESGPEVVRGRTEWRPKSANNSRSILEMPAESL SEQ ID NO: 130 CcFATB3b amino acid sequence MVATAAASAFFPVGAPATSSATSAKASMMPDNLDARGIKPKLASSSGLQVKANAHA 45 SPKINGSKVSTDTLKGEDTLTSSPAPRTFINQLPDWSMFLAAITTIFLAAEKQWTNLDWKPRRP DMLADPFGIGRFMQDGLIFRQHFAIRSYEIGADRTASIETLMNHLQETALNHVRSAGLLGDGF GATPEMSRRDLIWVVTRMQVLVDRYPAWGDIVEVETWVGASGKNGMRRDWLVRDSQTGEI LTRATSVWVMMNKRTRRLSKLPEEVRGEIGPYFIEDVAIIEEDNRKLQKLNENTADNVRRGLT PRWSDLDVNQHVNNVKYIGWILESAPGSILESHELSCMTLEYRRECGKDSVLQSMTAVSGGG 50 SAAGGSPESSVECDHLLQLESGPEVVRGRTEWRPKSANNSRSILEMPAESL 88 WO 2014/151904 PCT/US2014/026644 SEQ ID NO: 131 CcFATB3c amino acid sequence MVATAAASAFFPVGAPATSSATSAKASMMPDNLDARGIKPKPASSSGLQVKANAHA SPKINGSKVSTDTLKGEDTLTSSPAPRTFINQLPDWSMFLAAITTIFLAAEKQWTNLDWKPRRP 5 DMLADPFGIGRFMQDGLIFRQHFAIRSYEIGADRTASIETLMNHLQETALNHVRSAGLLGDGF GATPEMSRRDLIWVVTRMQVLVDRYPAWGDIVEVETWVGASGKNGMRRDWLVRDSQTGEI LTRATSVWVMMNKRTRRLSKLPEEVRGEIGPYFIEDVAIIEEDNRKLQKLNENTADNVRRGLT PRWSDLDVNQHVNNAKYIGWILESAPGSILESHELSCMTLEYRRECGKDSVLQSMTAVSGGG SAAGGSPESSVECDHLLQLESGPEVVRGRTEWRPKSANNSRSILEMPAESL 10 SEQ ID NO: 132 ChtFATBl a amino acid sequence MVAAAASSAFFSVPTPGTSTKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASAHPK ANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRPGMLVEP FGVDRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCK 15 RDLIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAM MNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGDSIRKGLTPRWNDLDVNQ HVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVLESVTAMDTAKEGDRSLYQHL LRLEDGADITIGRTEWRPKNAGANGAISTGKTSNENSVS SEQ ID NO: 133 20 ChtFATB 1a.1 amino acid sequence MVAAAASSAFFSVPTPGTSPKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASAHPK ANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRPGMLVEP FGVDRIFQDGVFFRHSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCK RDLIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLIGDCRTGEILIRATSVWAM 25 MNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGDSIRKGLTPRWNDLDVNQ HVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVLESVTAMDTAKEGDRSLYQHL LRLEDGADITIGRTEWRPKNAGANGALSTGKTSNGNSVS SEQ ID NO: 134 ChtFATBla.2 amino acid sequence 30 MVAAAASSAFFSVPTPGTSPKPGNFGNWPSNLSVPFKPESNHNGGFRVKANASAHPK ANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRPGMLVEP FGVDRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCK RDLIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAM MNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGDSIRKGLTPRWNDFDVNQ 35 HVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVLESVTAMDTAKEGDRSLYQHL LRLEDGADITIGRTEWRPKNAGANGAISTGKTSNENSVS SEQ ID NO: 135 ChtFATBl a.3 amino acid sequence MVAAAASSAFFSVPTPGTSPKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASAHPK 40 ANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRPGMLVEP FGVDRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCK RDLIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAM MNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGDSIRKGLTPRWNDFDVNQ HVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVLESVTAMDTAKEGDRSLYQHL 45 LRLEDGADITIGRTEWRPKNAGVNGAISTGKTSNENSVS SEQ ID NO: 136 ChtFATBla.4 amino acid sequence MVAAAASSAFFSVPTPGTSPKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASAHPK ANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWSMLLSKITTVFGAAERQWKRPGMLVEPF 50 GVDRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKR DLIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMM 89 WO 2014/151904 PCT/US2014/026644 NRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGDSIRKGLTPRWNDFDVNQHV NNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVLESVTAMDTAKEGDRSLYQHLLR LEDGADITIGRTEWRPKNAGANGAISTGKTSNENSVS SEQ ID NO: 137 5 ChtFATBlb amino acid sequence MVAAAASSAFFSVPTSGTSPKPGNFGNWPSSLSVPFKPESSHNGGFQVKANASAHPK ANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWSMLLSKITTVFWAAERQWKRPGMLVEP FGVDRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCK RDLIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAM 10 MNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGDFIRKGLTPRWNDFDVNQ HVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVLESVTAMDTAKEGDRSLYQHL LRLEDGADITIGRTEWRPKNAGANGAISTGKTSNENSVS SEQ ID NO: 138 ChtFATB2b amino acid sequence 15 MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHP KANGSAVSLKSGSLNTQEGTSSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQLTMLDRKSKK PDMHVDWFGLEIIVQDGLVFRESFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGF GRTPEMCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIR ATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPEW 20 NDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGD RSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS SEQ ID NO: 139 ChtFATB2a amino acid sequence MVVAAAASSAFFPVPAPGTTSKPGKFGNWPSSLSPSFKPKSNPNGGFQVKANASAHP 25 KANGSAVSLKSGSLNTKEDTPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQLTMLDRKSKK PDMHVDWFGLEIIVQDWLVFRESFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGF GRTPEMCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIR ATSIWAMMNQKTRRF SKLPNEVRQEIAPHFVDAPPLIEDNDRKLHKFDVKTGDSICKGLTPEW NDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGD 30 RSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS SEQ ID NO: 140 ChtFATB2c amino acid sequence MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHP KANGSAVSLKSGSLNTKEDTPSSPPPRTFLNQLPDWNRLRTAITTVFVAAEKQLTMLDRKSKK 35 PDMHVDWFGLEIIVQDGLVFRESFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGF GRTPEMCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIR ATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPEW NDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGD RSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS 40 SEQ ID NO: 141 ChtFATB2d amino acid sequence MVVAAAASSAFFPVPAPGTTSKPGKFGNWPSSLSPSFKPKSNPNGGFQVKANASAHP KANGSAVSLKSGSLNTQEDTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRP DMLVDLFGLESIVQDGLVFRESYSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFG 45 RTPEMCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRA TSIWAMMNQNTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSIRKGLTPGWN DLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRRECGRESVLESVTAMNPSKVGDRS QYQHLLRLEDGADIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS 50 90 WO 2014/151904 PCT/US2014/026644 SEQ ID NO: 142 ChtFATB2e amino acid sequence MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHP KANGSAVSLKSGSLNTQEDTSSSPPPQTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRP 5 DMLVDWFGLESIVQDGLVFRESYSIRSYEISADRTASIETVMNLLQETSLNHCKSMGILNDGFG RTPEMCKRDLIWVLTKMQILVNRYPNWGDTVEINSWF SQSGKIGMGRNWLISDCNTGEILIRA TSIWAMMNQNTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSIRKGLTPGWN DLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRRECGRDSVLESVTAMNPSKVGDRS QYQHLLRLEDGADIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS 10 SEQ ID NO: 143 ChtFATB2f amino acid sequence MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHP KANGSAVSLKSGSLNTQEGTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRP DMLVDWFGLESIVQDGLVFRESYSIRSYEISADRTASIETVMNLLQETSLNHCKSMGILNDGFG 15 RTPEMCKRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRA TSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPEWN DLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGDR SQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS SEQ ID NO: 144 20 ChtFATB2g amino acid sequence MVVAATASSAFFPVPVPGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPK ANGSAVSLKSGSLNTQEDTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRPD MLVDWFGLESIVQDGLVFREIYSIRSYEISADRTTSIETVMNLLQETSLNHCKSMGILNDGFGRT PEMCKRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSI 25 WAMMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPEWNDL DVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGDRSQ YQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNANSVS SEQ ID NO: 145 ChtFATB2h amino acid sequence 30 MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHP KANGSAVSLKSGSLNTQEGTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRP DMLVDWFGLESIVQDGLVFRESYSIRSYEISADRTASIETVMNLLQETSLNHCKSMGILNDGFG RTPEMCKRDLIWVLTKMQILVNRYPNWGDTVEINSWF SQSGKIGMGRNWLISDCNTGEILIRA TSIWAMMNQNTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSIRKGLTPGWN 35 DLDVNQHVSNVKYIGWILESIPTEVLETQELCSLTLEYRRECGRESVLESVTAMNPSKVGDRSQ YQHLLRLEDGADIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS SEQ ID NO: 146 ChtFATB3a amino acid sequence MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKIN 40 GSSVSLKSCSLKTHEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNEGFG RTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEIL TRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKLPKLDEKTADSIRKGLTP KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKG 45 FGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIASGETSPGNS SEQ ID NO: 147 ChtFATB3b amino acid sequence MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKIN GSSVSLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD 50 MLVDPFGFGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLIEGFGR TPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILT 91 WO 2014/151904 PCT/US2014/026644 RASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKLPKLDEKTADSIRKGLTPK WNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKGF GPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIASGETSPGNS SEQ ID NO: 148 5 ChtFATB3c amino acid sequence MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKIN GSSVSLKSCSLKTHEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNEGFG RTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEIL 10 TRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTP KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSEKG FGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGAIAFGETSPGDS SEQ ID NO: 149 ChtFATB3d amino acid sequence 15 MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKIN GSSVSLKSCSLKTHEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIKTVMNHLQETALNHVKSAGLLNEGFG RTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEIL TRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKLPKLDEKTADSIRKGLTP 20 KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKG FGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIASGETSPGNS SEQ ID NO: 150 ChtFATB3e amino acid sequence MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKIN 25 GSSVSLKSGSLKTHEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNEGFG RTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEIL TRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKLPKLDEKTADSIRKGLTP KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKG 30 FGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIASGETSPGNS SEQ ID NO: 151 ChtFATB3f amino acid sequence MVATAASSAFFPVPSPDTSSRLGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKIN GSSVSLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD 35 MPVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNEGFG RTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEIL TRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKLPKLDEKTADSIRKGLTP KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSEKG FGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIASGETSPGNS 40 SEQ ID NO: 152 ChtFATB3g amino acid sequence MVATAASSAFFPVPSPDTSSRAGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKIN GSSVSLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNEGFG 45 RTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEIL TRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKLPKLDEKTADSIRKGLTP KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKG FGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIASGETSPGNS 50 92 WO 2014/151904 PCT/US2014/026644 SEQ ID NO: 153 ChsFATB1 amino acid sequence MVATNAAAFSAYTFFLTSPTHGYSSKRLADTQNGYPGTSLKSKSTPPPAAAAARNGA LPLLASICKCPKKADGSMQLDSSLVFGFQFYIRSYEVGADQTVSIQTVLNYLQEAAINHVQSAG 5 YFGDSFGATPEMTKRNLIWVITKMQVLVDRYPAWGDVVQVDTWTCSSGKNSMQRDWFVRD LKTGDIITRASSVWVLMNRLTRKLSKIPEAVLEEAKLFVMNTAPTVDDNRKLPKLDGSSADYV LSGLTPRWSDLDMNQHVNNVKYIAWILESVPQSIPETHKLSAITVEYRRECGKNSVLQSLTNV SGDGITCGNSIIECHHLLQLETGPEILLARTEWISKEPGFRGAPIQAEKVYNNK SEQ ID NO: 154 10 ChsFATB2 amino acid sequence MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAPPKIN GSSVGLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFG RTLEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEI 15 LTRASSVWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTP KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKG SGSQFQHLLRLEDGGEIVKGRTEWRPKTAGINGPIASGETSPGDSS SEQ ID NO: 155 ChsFatB2b amino acid sequence 20 MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAPPKIN GSSVGLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFG RTLEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEI LTRASSKSQIMLPLHYCSVWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLD 25 EKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESV LESLTAVDPSGKGSGSQFQHLLRLEDGGEIVKGRTEWRPKTAGINGPIASGETSPGDSS SEQ ID NO: 156 ChsFatB2c amino acid sequence MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAPPKIN 30 GSSVGLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFG RTLEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEI LTRASSVWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTP KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKG 35 SGSQFQHLMRLEDGGEIVKGRTEWRPKTAGINGPIASGETSPGDSS SEQ ID NO: 157 ChsFatB2d amino acid sequence MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAPPKIN GSSVGLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD 40 MLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFG RTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEIL TRASSVWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTP KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKG SGSQFQHLLRLEDGGEIVKGRTEWRPKTAGINGPIASGETSPGDSS 45 SEQ ID NO: 158 Chs FATB3 amino acid sequence MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASARPK ANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRP DMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFG 50 RTPEMCKRDLIWVVTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDCHTGEILIR ATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRW 93 WO 2014/151904 PCT/US2014/026644 NDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRRECGRDSVLESVTAMDPSKEGD RSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS SEQ ID NO: 159 ChsFatb3b amino acid sequence 5 MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASARPK ANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRP DMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFG RTPEMCKRDLIWVVTKMHIEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDFHTGDILIR ATSVCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRW 10 NDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRRECGRDSVLESVTAMDPSKEGD RSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS SEQ ID NO: 160 ChsFatB3c amino acid sequence MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASARPK 15 ANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRP DMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFG RTPEMCKRDLIWVVTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDCHTGEILIR ATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRW NDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRQECGRDSVLESVTAMDPSKEGD 20 RSLYQHLLRLEDGTDIAKGRTKWRPKNAGKTSNGNSIS SEQ ID NO: 161 ChsFATB3d amino acid sequence MVAAEASSALFSVRTPGTSPKPGKFGNWPSSLSVPFKSKSNHNGGFQVKANASARPK ANGSAVSLKSGSLDTQEDASSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKR 25 SDMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGF GRTPEMCKRDLIWVVTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDCHTGEILI RATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPR WNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRRECGRDSVLESVTAMDPSKEG DRSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS 30 SEQ ID NO: 162 ChsFATB3e amino acid sequence MVAAEASSALFSVRTPGTSPKPGKFGNWPSSLSVPFKSKSNHNGGFQVKANASARPK ANGSAVSLKSGSLDTQEDASSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKR SDMLMDPFGVDRVVQDGVVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGF 35 GRTPEMCKRDLIWVVTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDCHTGEILI RATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPR WNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRRECGRDSVLESVTAMDPSKEG DRSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS SEQ ID NO: 163 40 ChsFATB3f amino acid sequence MVAAEASSALFSVRTPGTSPKPGKFGNWPSSLSVPFKSKSNHNGGFQVKANASARPK ANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRP DMLMDPFGVDRVVQDGAVFRQSF SIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFG RTPEMCKRDLIWVVTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDCHTGEILIR 45 ATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRW NDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRRECGRDSVLESVTAMDPSKEGD RSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS SEQ ID NO: 164 ChsFATB3g amino acid sequence 50 MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASARPK 94 WO 2014/151904 PCT/US2014/026644 ANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRP DMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFG RTPEMCKRDLIWVVTKMHIEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDFHTGDILIR ATSVCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRW 5 NDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRQECGRDSVLESVTAMDPSKEGD RSLYQHLLRLEDGTDIAKGRTKWRPKNAGKTSNGNSIS SEQ ID NO: 165 ChsFATB3h amino acid sequence MVAAEASSALFSVRTPGTSPKPGKFGNWPSSLSVPFKSKSNHNGGFQVKANASARPK 10 ANGSAVSLKSGSLDTQEDASSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKR SDMLMDPFGVDRVVQDGVVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGF GRTPEMCKRDLIWVVTKMHIEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDFHTGDILI RATSVCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPR WNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRQECGRDSVLESVTAMDPSKEG 15 DRSLYQHLLRLEDGTDIAKGRTKWRPKNAGKTSNGNSIS SEQ ID NO: 166 ChsFATB3i amino acid sequence MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASARPK ANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRP 20 DMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFG RTPEMCKRDLIWVVTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDCHTGEILIR ATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRW NDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRRECGGDSVLESVTAMDPSKEGD RSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS 25 SEQ ID NO: 167 ChsFATB3i amino acid sequence MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASARPK ANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRP DMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFG 30 RTPEMCKRDLIWVVTKMHIEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDFHTGDILIR ATSVCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRW NDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRQECGRDSVLESVTAMDPSKEGD RSLYQHLLRLEDGTDIAKGRTKWRPKNAGKTSNGNSIS SEQ ID NO: 168 35 CcalcFATB1 (Cuphea calcarata FATB1) MVAAAATSAFFPVPAPGTSPNPRKFGSWPSSLSPSLPKSIPNGGFQVKANASAHPKANGSAVSL KSGSLNTQENTSSSPPPRTFLHQLPDWSRLLTAITTVFVKSKRPDMHDRKSKRPDMLVDLFGLE SSVQDALVFRQSF SIRSYEIGTDRTASIETLMNHLQETSLNHCKSTGILLDGFGRTLEMCKRELI WVVIKMQIQVNRYPAWGDTVEINTRF SRLGKIGMGRDWLISDCNTGEILIRATSEYAMMNQK 40 TRRLSKLPYEVHQEIAPLFVDSPPVIEDNDLKVHKFEVKTGDSIQKGLSPGWNDLDVNQHVSN VKYIGWILESMPTEVLETQELCSLALEYRRECGRDSVLESVTAMDPSKVGGRSQYQHLLRLED GTAIVNGITEWRPKNAGANGAISTGKTSNGNSVS SEQ ID NO: 169 ChookFATB4 (Cuphea hookeriana FATB4) 45 MVAAAATSAFFPVPAPGTSPNPRKFGSWPSSLSPSLPNSIPNGGFQVKANASAHPKANGSAVSL KSGSLNTQENTSSSPPPRTFLHQLPDWSRLLTAITTVFVKSKRPDMHDRKSKRPDMLVDLFGLE SSVQDALVFRQRFSIRSYEIGTDRTASMETLMNHLQETSLNHCKSTGILLDGFGRTLEMCKREL IWVVIKMQIQVNRYPAWGDTVEINTRFSRLGKIGMGRDWLISDCNTGEILIRATSEYAMMNQK TRRLSKLPYEVRQEIAPLFVDSPPVIEDNDLKVHKFEVKTGDSIHKGLTPGWNDLDVNQHVNN 95 WO 2014/151904 PCT/US2014/026644 VKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAMDPSGGGYGSQFQHLLRLEDG GEIVKGRTEWRPKNGVINGVVPTGESSPGDYS SEQ ID NO: 170 CaFATB 1 (Cuphea avigera var. pulcherrima FATB 1) 5 MVAAAASSAFFSVPVPGTSPKPGKFRIWPSSLSPSFKPKPIPNGGLQVKANSRAHPKANGSAVS LKSGSLNTQEDTSSSPPPRTFLHQLPDWSRLLTAITTVFVKSKRPDMHDRKSKRPDMLMDSFG LESIVQEGLEFRQSFSIRSYEIGTDRTASIETLMNYLQETSLNHCKSTGILLDGFGRTPEMCKRDL IWVVTKMKIKVNRYPAWGDTVEINTWF SRLGKIGKGRDWLISDCNTGEILIRATSAYATMNQ KTRRLSKLPYEVHQEIAPLFVDSPPVIEDNDLKLHKFEVKTGDSIHKGLTPGWNDLDVNQHVS 10 NVKYIGWILESMPTEVLETQELCSLALEYRRECGRDSVLESVTAMDPTKVGGRSQYQHLLRLE DGTDIVKCRTEWRPKNPGANGAISTGKTSNGNSVS SEQ ID NO: 171 CpauFATB 1 (Cuphea paucipetala FATB 1) MVAAAASSAFFPVPAPGTSPKPGKSGNWPSSLSPSIKPMSIPNGGFQVKANASAHPKANGSAV 15 NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMRDRKSKRPDMLVD SVGLKSVVLDGLVSRQIFSIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMC KNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSHSGKIGMASDWLITDCNTGEILIRATSVWA MMNQKTRRFSRLPYEVRQELTPHYVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLDV NQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAMDPSEDEGRSQYKH 20 LLRLEDGTDIVKGRTEWRPKNAGTNGAISTAKPSNGNSVS SEQ ID NO: 172 CprocFATB 1 (Cuphea procumbens FATB 1) MVAAAASSAFFPAPAPGSSPKPGKSGNWPSSLSPSFKSKSIPYGRFQVKANASAHPKANGSAV NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVD 25 SVGLKNIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGM CKNDLIWVLTKMQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVW AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLD VNQHVSNVKYIGWILESMPIEVLEAQELCSLTVEYRRECGMDSVLESVTAVDPSEDGGRSQYN HLLRLEDGTDVVKGRTEWRPKNAETNGAISPGNTSNGNSIS 30 SEQ ID NO: 173 CprocFATB2 (Cuphea procumbens FATB2) MVAAAASSAFFPAPAPGSSPKPGKSGNWPSSLSPSFKSKSIPYGRFQVKANASAHPKANGSAV NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVD SVGLKNIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGM 35 CKNDLIWVLTKMQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVW AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLD VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRQECGRESVLESLTAVDPSGKGFGSQFQH LLRLEDGGEIVKGRTEWRPKTAGINGAIASGETSPGDF SEQ ID NO: 174 40 CprocFATB3 (Cuphea procumbens FATB3) MVAAAASSAFFPAPAPGSSPKPGKSGNWPSSLSPSFKSKSIPYGRFQVKANASAHPKANGSAV NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVD SVGLKNIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGM CKNDLIWVLTKMQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVW 45 AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLD VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGEGGYGSQFQ HLLRLEDGGEIVKGRTEWRPKNAGINGVLPTGE 96 WO 2014/151904 PCT/US2014/026644 SEQ ID NO: 175 CigneaFATB1 (Cuphea ignea FATBi) PGTSRKTGKFGNWPSSLSPSFKPKSIPNGGFQVKANARAHPKANGSAVSLKSVSLNTQEDTSLS PPPRAFLNQLPDWRMLRTALTTVFVAAEKQWTMLDRKSKRPDMLVDSFGLESIVQEGLVFRQ 5 SFSIRSYEIGIDRTASIETLMNHLQETSLNQCKSAGILHDGFGRTLEMCKRDLIWVVTKMQIKV NRYPAWGDTVEISTRFSRLGKIGMGRDWLICDCNTGEILIRATSAYAMMNQKTRRLSKLPNEV RQEIAPLFVDSDPVIEENDMKLHKFEVKTGDSICKGLTPRWSDLDVNQHVSNVKYIGWILESM PTEVLETQELCSLALEYRRECGRDSVLESVTSMDPSKVGGWSQYQHLLRLEDGADIVKGRTE WRPKNAGANGAISTGKT 10 SEQ ID NO: 176 CcalcFATB1 (Cuphea calcarata FATB1) |MATASTFSAFNARCGDLRRSAGSGPRRPARPLPVRAAI NASAHPKANGSAVSLKSGSLETQED NSSSSRPPRTFIKQLPDWSMLLSAITTVFVAAEKQWTMFDRKSKRSDMLVDPFVVDRIVQDGV LFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSMGLLYEGFGRTPEMCKRDLIWVVTKIH 15 IKVNRYPTWGDTIEVTTWVSESGKNGMGRDWLISDCHTGEILIRATSVWAMMNQTTRRLSKF PYELRQEIAPHFVDSDPVIEDNRRLLNFDVKTGDSIRKGLTPRWNDLDVNQHVNNVKYIGWIL ESVPTEVFDTRELCGLTLEYRQECGRGSVLESVTAMDPSKEGDRSLYQHLLRLEDGTDIVKGR TEWRPKNAGTNGPVSTRKTTNGSSVS SEQ ID NO: 177 20 ChookFATB4 (Cuphea hookeriana FATB4) |MATASTFSAFNARCGDLRRSAGSGPRRPARPLPVRAAINASAHPKANGSAVSLKSGSLNTQEN TSSSPPPRTFLHQLPDWSRLLTAITTVFVKSKRPDMHDRKSKRPDMLVDLFGLESSVQDALVFR QRFSIRSYEIGTDRTASMETLMNHLQETSLNHCKSTGILLDGFGRTLEMCKRELIWVVIKMQIQ VNRYPAWGDTVEINTRF SRLGKIGMGRDWLISDCNTGEILIRATSEYAMMNQKTRRLSKLPYE 25 VRQEIAPLFVDSPPVIEDNDLKVHKFEVKTGDSIHKGLTPGWNDLDVNQHVNNVKYIGWILES TPPEVLETQELCSLTLEYRRECGRESVLESLTAMDPSGGGYGSQFQHLLRLEDGGEIVKGRTE WRPKNGVINGVVPTGESSPGDYS SEQ ID NO: 178 CaFATB 1 (Cuphea avigera var. pulcherrima FATB 1) 30 |MATASTFSAFNARCGDLRRSAGSGPRRPARPLPVRAAI SRAHPKANGSAVSLKSGSLNTQED TSSSPPPRTFLHQLPDWSRLLTAITTVFVKSKRPDMHDRKSKRPDMLMDSFGLESIVQEGLEFR QSFSIRSYEIGTDRTASIETLMNYLQETSLNHCKSTGILLDGFGRTPEMCKRDLIWVVTKMKIK VNRYPAWGDTVEINTWFSRLGKIGKGRDWLISDCNTGEILIRATSAYATMNQKTRRLSKLPYE VHQEIAPLFVDSPPVIEDNDLKLHKFEVKTGDSIHKGLTPGWNDLDVNQHVSNVKYIGWILES 35 MPTEVLETQELCSLALEYRRECGRDSVLESVTAMDPTKVGGRSQYQHLLRLEDGTDIVKCRTE WRPKNPGANGAISTGKTSNGNSVS SEQ ID NO: 179 CpauFATB 1 (Cuphea paucipetala FATB 1) |MATASTFSAFNARCGDLRRSAGSGPRRPARPLPVRAAI NASAHPKANGSAVNLKSGSLNTQE 40 DTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMRDRKSKRPDMLVDSVGLKSVVLDG LVSRQIFSIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIWVLTK MQIMVNRYPTWGDTVEINTWFSHSGKIGMASDWLITDCNTGEILIRATSVWAMMNQKTRRFS RLPYEVRQELTPHYVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLDVNQHVSNVKYI GWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAMDPSEDEGRSQYKHLLRLEDGTDI 45 VKGRTEWRPKNAGTNGAISTAKPSNGNSVS 97 WO 2014/151904 PCT/US2014/026644 SEQ ID NO: 180 CprocFATB 1 (Cuphea procumbens FATB 1) |MATASTFSAFNARCGDLRRSAGSGPRRPARPLPVRAAI NASAHPKANGSAVNLKSGSLNTQE DTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVDSVGLKNIVRDG 5 LVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIWVLTK MQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVWAMMNQKTRRFS RLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLDVNQHVSNVKYI GWILESMPIEVLEAQELCSLTVEYRRECGMDSVLESVTAVDPSEDGGRSQYNHLLRLEDGTDV VKGRTEWRPKNAETNGAISPGNTSNGNSIS 10 SEQ ID NO: 181 CprocFATB2 (Cuphea procumbens FATB2) |MATASTFSAFNARCGDLRRSAGSGPRRPARPLPVRAAI NASAHPKANGSAVNLKSGSLNTQE DTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVDSVGLKNIVRDG LVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIWVLTK 15 MQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVWAMMNQKTRRFS RLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLDVNQHVNNVKYI GWILESTPPEVLETQELCSLTLEYRQECGRESVLESLTAVDPSGKGFGSQFQHLLRLEDGGEIV KGRTEWRPKTAGINGAIASGETSPGDF SEQ ID NO: 182 20 CprocFATB3 (Cuphea procumbens FATB3) |MATASTFSAFNARCGDLRRSAGSGPRRPARPLPVRAAI NASAHPKANGSAVNLKSGSLNTQE DTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVDSVGLKNIVRDG LVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIWVLTK MQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVWAMMNQKTRRFS 25 RLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLDVNQHVNNVKYI GWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGEGGYGSQFQHLLRLEDGGEI VKGRTEWRPKNAGINGVLPTGE SEQ ID NO: 183 CigneaFATB1 (Cuphea ignea FATBi) 30 |MATASTFSAFNARCGDLRRSAGSGPRRPARPLPVRAAI NARAHPKANGSAVSLKSVSLNTQED TSLSPPPRAFLNQLPDWRMLRTALTTVFVAAEKQWTMLDRKSKRPDMLVDSFGLESIVQEGL VFRQSFSIRSYEIGIDRTASIETLMNHLQETSLNQCKSAGILHDGFGRTLEMCKRDLIWVVTKM QIKVNRYPAWGDTVEISTRFSRLGKIGMGRDWLICDCNTGEILIRATSAYAMMNQKTRRLSKL PNEVRQEIAPLFVDSDPVIEENDMKLHKFEVKTGDSICKGLTPRWSDLDVNQHVSNVKYIGWI 35 LESMPTEVLETQELCSLALEYRRECGRDSVLESVTSMDPSKVGGWSQYQHLLRLEDGADIVK GRTEWRPKNAGANGAISTGKT SEQ ID NO: 184 CgFATB 1 (Cuphea glossostoma FATB 1)_ MVAAAASSAFFPSPAPGSSPKPGNRPSSLSPSFKPKSIPNGAFQVKANASAHPKANGSAVNLKSI 40 GSLNTQEDSSSSPSPRAFLNQLPDWSVLLTAITTVFVAAEKQWTMLDRKSKRPDVLVDSVGLK SIVQDGLVSRQSFSIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDL WVLTKMQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVWAMMNQ KTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLDVNQHVS NVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAMDPSEDGGRSQYNHLLRLE 45 DGTDVVKGRTEWRPKNAGTNGAISTTKTSNGNSVS 98 WO 2014/151904 PCT/US2014/026644 SEQ ID NO: 185 CgFATBlb (Cuphea glossostoma FATB1 C170F,M198T,T374S variant) MVAAAASSAFFPSPAPGSSPKPGNRPSSLSPSFKPKSIPNGAFQVKANASAHPKANGSAVNLKS GSLNTQEDSSSSPSPRAFLNQLPDWSVLLTAITTVFVAAEKQWTMLDRKSKRPDVLVDSVGLK 5 SIVQDGLVSRQSFSIRSYEIGADRTASIETLMNHLQETSINHFKSLGLLNDGFGRTPGMCKNDLI WVLTKTQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVWAMMNQ KTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLDVNQHVS NVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVSAMDPSEDGGRSQYNHLLRLE DGTDVVKGRTEWRPKNAGTNGAISTTKTSNGNSVS 10 SEQ ID NO: 186 Umbellularia californica UcFATB3 amino acid sequence MVATAAASAFFPVGSPATSSATSAKASMMPDNLDARGIKPKPASSSGLQVKANAHASPKINGS KVSTDTLKGEDTLTSSPAPRTFINQLPDWSMFLAAITTIFLAAEKQWTNLDWKPRRPDMLADP 15 FGIGRFMQDGLIFRQHFAIRSYEIGADRTASIETLMNHLQETALNHVRSAGLLGDGFGATPEMS RRDLIWVVTRMQVLVDRYPAWGDIVEVETWVGASGKNGMRRDWLVRDSQTGEILTRATSV WVMMNKRTRRLSKIPEEVRGEIGPYFMENVAIIEEDSRKLQKLNENIIEEDSRKLQKLNENTAD NVRRGLTPRWSDLDVNQHVNNVKYIGWILESAPGSILESHELSCMTLEYRRECGKDSVLQSM TVVSGGGSAAGGSPESSVECDHLLQLESGPEVVKARTEWRPKSANNPRSILEMPAESS* 20 SEQ ID NO: 187 Cuphea carthagenensis CCrFATB2c (V138L variant of FATB2) MVAAAASSAFFPVTTPGTSRKPGKFGNWLSSLSPPFRPKSIPSGGFQVKANASAHPKANGSAV SLKSGSLNTQEDTSSSPPPRAFINQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRSDMLVDS 25 FGMERIVQDGLLFRQSFSIRSYEIGADRRASIETLMNHLQETSLNHCKSIRLLNEGFGRTPEMCK RDLIWVVTRMHIMVNRYPTWGDTVEINTWVSQSGKNGMGRDWLISDCNTGEILIRATSAWA MMNQKTRRLSKLPYEVSQEIAPHFVDSPPVIEDGDRKLHKFDVKTGDSIRKGLTPRWNDLDV NQHVNNVKYIGWILESMPTEVLETHELCFLTLEYRRECGRDSVLESVTAMDPSNEGGRSHYQ HLLRLEDGTDIVKGRTEWRPKNARNIGAISTGKTSNGNPAS* 30 SEQ ID NO: 188 Cuphea carthagenensis CCrFATB2 MVAAAASSAFFPVTTPGTSRKPGKFGNWLSSLSPPFRPKSIPSGGFQVKANASAHPKANGSAV SLKSGSLNTQEDTSSSPPPRAFINQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRSDMLVDS 35 FGMERIVQDGLVFRQSFSIRSYEIGADRRASIETLMNHLQETSLNHCKSIRLLNEGFGRTPEMCK RDLIWVVTRMHIMVNRYPTWGDTVEINTWVSQSGKNGMGRDWLISDCNTGEILIRATSAWA MMNQKTRRLSKLPYEVSQEIAPHFVDSPPVIEDGDRKLHKFDVKTGDSIRKGLTPRWNDLDV NQHVNNVKYIGWILESMPTEVLETHELCFLTLEYRRECGRDSVLESVTAMDPSNEGGRSHYQ HLLRLEDGTDIVKGRTEWRPKNARNIGAISTGKTSNGNPAS* 40 SEQ ID NO: 189 CcrFATB2b MVAAAASSAFFPVTTPGTSRKPGKFGNWLSSLSPPFRPKSIPSGGFQVKANASAHPKANGSAV 45 SLKSGSLNTQEDTSSSPPPRAFINQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRSDMLVDS FGMERIVQDGLVFRQSFSIRSYEIGADRRASIETLMNHLQETSLNHCKSIRLLNEGFGRTPEMCK RDLIWVFTRMHIMVNRYPTWGDTVEINTWVSQSGKNGMGRDWLISDCNTGEILIRATSAWA MMNQKTRRLSKLPYEVSQEIAPHFVDSPPVIEDGDRKLHKFDVKTGDSIRKGLTPRWNDLDV NQHVNNVKYIGWILESMPTEVLETHELCFLTLEYRRECGRDSVLESVTAMDPSNEGGRSHYQ 50 HLLRLEDGTDIVKGRTEWRPKNARNIGAIPTGKTSNGNPAS* 99 WO 2014/151904 PCT/US2014/026644 SEQ ID NO: 190 CcrFATB1 MVATAASSAFFPVPSPDSSSRPGKLGNGPSSLSPLKPKSTPNGGLQVKANASAPPKINGSSVGL 5 KSSSLKTQDDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLTDPF GLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFGRTPEMY KRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSV WVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTPKWNDLD VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGKESVLESLTAVDPSGKGWGSHFQ 10 HLLRLEDGGEIVKGRTEWRPKNAGINGAVAFEETSPGDS* SEQ ID NO: 191 CcrFATBlb 15 MVATAASSAFFPVPSPDSSSRPGKLGNGPSSLSPLKPKSTPNGGLQVKANASAPPKINGSSVGL KSSSLKTQDDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLTDPF GLGRIAQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFGRTPEMY KRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSV WVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTPKWNDLD 20 VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGKESVLESLTAVDPSGKGWGSHFQ HLLRLEDGGEIVKGRTEWRPKNAGINGAVAFEETSPGDS* SEQ ID NO: 192 CCrFATBl c 25 MVATAASSAFFPVPSPDSSSRPGKLGNGPSSLSPLKPKSTPNGGLQVKANASAPPKINGSSVGL KSSSLKTQDDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLTDPF GLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFGRTPEMY KRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSV WVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTPKWNDLD 30 VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGKESVLESLTAVDPSGKGWGSHFQ HLLRLEDGGEIVKGRTEWRPKNA* 100