AU2018267601A1 - Thioesterases and cells for production of tailored oils - Google Patents

Abstract

The invention features plant acyl-ACP thioesterase genes of the FatB class and proteins encoded by these genes. The genes are useful for constructing recombinant host cells having altered fatty acid profiles. Oleaginous microalga host cells with the new genes or previously identified FatB genes are disclosed. The microalgae cells produce triglycerides with useful fatty acid profiles.

Description

THIOESTERASES AND CELLS FOR PRODUCTION OF TAILORED OILS

Cross Reference to related Applications [0001] This is a divisional application of Australian Patent Application No.

2014236763, the entire contents of which are incorporated herein by reference.

Background [0002] Certain organisms including plants and some microalgae use a type II fatty acid biosynthetic pathway, characterized by the use of discrete, mono functional enzymes for fatty acid synthesis. In contrast, mammals and fungi use a single, large, 10 multifunctional protein.

[0003] Type II fatty acid biosynthesis typically involves extension of a growing acyl-ACP (acyl-carrier protein) chain by two carbon units followed by cleavage by an acyl-ACP thioesterase. In plants, two main classes of acyl-ACP thioesterases have been identified: (i) those encoded by genes of the FatA class, 15 which tend to hydrolyze oleoyl-ACP into oleate (an 18:1 fatty acid) and ACP, and (ii) those encoded by genes of the FatB class, which liberate C8-C16 fatty acids from corresponding acyl-ACP molecules.

[0004] Different FatB genes from various plants have specificities for different acyl chain lengths. As a result, different gene products will produce different fatty acid profiles in plant seeds. See, US Patent Nos. 5,850,022; 5,723,761;

5,639,790; 5,807,893; 5,455,167; 5,654,495; 5,512,482;5,298,421;5,667,997; and

5,344,771; 5,304,481. Recently, FatB genes have been cloned into oleaginous microalgae to produce triglycerides with altered fatty acid profiles. See, WO2010/063032, WO2011/150411, W02012/106560, and WO2013/158938.

2018267601 21 Nov 2018

Summary [0005] In various aspects, the invention(s) contemplated herein may include, but need not be limited to, any one or more of the following embodiments:

[0006] Embodiment 1: A nucleic acid construct including a regulatory element and a FatB gene expressing an active acyl-ACP thioesterase operable to produce an altered fatty acid profile in an oil produced by a cell expressing the nucleic acid construct, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 5 of Table la, the sequence having at least 94.6% sequence identity with each of SEQ ID NOs: 88, 82, 85, and 103, and optionally wherein the fatty acid of the oil is enriched in C8 and C10 fatty acids.

[0007] Embodiment 2: A nucleic acid construct including a regulatory element and a FatB gene expressing an active acyl-ACP thioesterase operable to produce an altered fatty acid profile in an oil produced by a cell expressing the nucleic acid construct, wherein the FatB gene expresses a protein having an amino acid sequence falling within one of clades 1-12 of Table la.

[0008] Embodiment 3: The nucleic acid construct of embodiment 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade of Table la, the sequence having at least 85.9% sequence identity with each of SEQ ID NOs: 19, 161, 22, and 160, and optionally wherein the fatty acid of the oil is enriched in C14 and C16 fatty acids.

[0009] Embodiment 4: The nucleic acid construct of embodiment 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade of Table la, the sequence having at least 89.5% sequence identity with each of SEQ ID NOs: 134-136, 132, 133, 137, 124, 122, 123, 125, and optionally wherein the fatty acid of the oil is enriched in C12 and C14 fatty acids.

[0010] Embodiment 5: The nucleic acid construct of embodiment 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade of Table la, the sequence having at least 92.5% sequence identity with each of SEQ ID NOs: 126 and 127, and optionally wherein the fatty acid of the oil is enriched in

C12 and C14 fatty acids.

[0011] Embodiment 6: The nucleic acid construct of embodiment 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade

2018267601 21 Nov 2018 of Table la, the sequence having at least 83.8% sequence identity with SEQ ID NO: 79, and optionally wherein the fatty acid of the oil is enriched in C12 and C14 fatty acids.

[0012] Embodiment 7: The nucleic acid construct of embodiment 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade of Table la, the sequence having at least 99.9% sequence identity with each of SEQ ID NOs: 111 and 110, and optionally wherein the fatty acid of the oil is enriched in C10 fatty acids.

[0013] Embodiment 8: The nucleic acid construct of embodiment 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade of Table la, the sequence having at least 89.5% sequence identity with each of SEQ ID NOs: 73, 106, 185, 172, 171, 173, 174, and optionally wherein the fatty acid of the oil is enriched in C10 and C12 fatty acids.

[0014] Embodiment 9: The nucleic acid construct of embodiment 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade of Table la, the sequence having at least 85.9% sequence identity with each of SEQ ID NOs: 112, 113, 142, 145, 143, 144, 139, 140, 138, 141, and optionally wherein the fatty acid of the oil is enriched in C12 and C14 fatty acids.

[0015] Embodiment 10: The nucleic acid construct of embodiment 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 9 of Table la, the sequence having at least 83.8% sequence identity with each of SEQ ID NOs: 187-189, and optionally wherein the fatty acid of the oil is enriched in C12 and C14 fatty acids.

[0016] Embodiment 11: The nucleic acid construct of embodiment 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 10 of Table la, the sequence having at least 95.9% sequence identity with each of SEQ ID NOs: 147, 149, 146, 150, 152, 151, 148, 154, 156, 155, 157, 108, 75, 190, 191, and 192, and optionally wherein the fatty acid of the oil is enriched in C14 and C16 fatty acids.

[0017] Embodiment 12: The nucleic acid construct of embodiment 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 11 of Table la, the sequence having at least 88.7% sequence identity

2018267601 21 Nov 2018 with SEQ ID NO: 121, and optionally wherein the fatty acid of the oil is enriched in C14 and C16 fatty acids.

[0018] Embodiment 13: The nucleic acid construct of embodiment 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 12 of Table la, the sequence having at least 72.8% sequence identity with each of SEQ ID NOs: 129 and 186, and optionally wherein the fatty acid of the oil is enriched in C16 fatty acids.

[0019] Embodiment 14: An isolated nucleic acid or recombinant DNA construct including a nucleic acid, wherein the nucleic acid has at least 80% sequence 10 identity to any ofSEQ ID NOS: 2, 3, 5, 6, 8, 9, 11, 12, 14, 15, 17, 18,20,21,23,24,

26, 27, 29, 30, 32, 33, 35, 36, 38, 39, 41, 42, 44, 45, 47, 48, 50, 51, 53, 54, 56, 57, 59,

60, 62, 63, 65, 66, 68, 69, 71, 72, 74, 76, 78, 80, 81, 83, 84, 86, 87, 89, 90, 92, 93, 95, 96, 98, 99, 101, 102, 104, 105, 107, 109 or any equivalent sequences by virtue of the degeneracy of the genetic code.

[0020] Embodiment 15: An isolated nucleic acid sequence encoding a protein or a host cell expressing a protein having at least 80% sequence identity to any ofSEQ ID NOS: 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 75, 77, 79, 82, 85, 88, 91, 94, 97, 100, 103, 106, 108, 110-192 or a fragment thereof having acyl-ACP thioesterase activity.

[0021] Embodiment 16: The isolated nucleic acid of embodiment 15, wherein, the protein has acyl-ACP thioesterase activity operable to alter the fatty acid profile of an oil produced by a recombinant cell including that sequence.

[0022] Embodiment 17: A method of producing a recombinant cell that produces an altered fatty acid profile, the method including transforming the cell with 25 a nucleic acid according to any of embodiments 1-3.

[0023] Embodiment 18: A host cell produced by the method of embodiment

17.

[0024] Embodiment 19: The host cell of embodiment 18, wherein the host cell is selected from a plant cell, a microbial cell, and a microalgal cell.

[0025] Embodiment 20: A method for producing an oil or oil-derived product, the method including cultivating a host cell of embodiment 5 or 6, and

2018267601 21 Nov 2018 extracting oil produced thereby, optionally wherein the cultivation is heterotrophic growth on sugar.

[0026] Embodiment 21: The method of embodiment 20, further including producing a fatty acid, fuel, chemical, or other oil-derived product from the oil.

[0027] Embodiment 22: An oil produced by the method of embodiment 20, optionally having a fatty acid profile including at least 20% C8, C10, C12, C14 or C16 fatty acids.

[0028] Embodiment 23: An oil-derived product produced by the method of embodiment 21.

[0029] Embodiment 24: The oil of embodiment 23, wherein the oil is produced by a microalgae and optionally, lacks C24-alpha sterols.

Description of Illustrative Embodiments of the Invention

Definitions [0030] As used with respect to nucleic acids, the term “isolated” refers to a nucleic acid that is free of at least one other component that is typically present with the naturally occurring nucleic acid. Thus, a naturally occurring nucleic acid is isolated if it has been purified away from at least one other component that occurs naturally with the nucleic acid.

[0031] A “natural oil” or “natural fat” shall mean a predominantly triglyceride oil obtained from an organism, where the oil has not undergone blending with another natural or synthetic oil, or fractionation so as to substantially alter the fatty acid profile of the triglyceride. In connection with an oil comprising triglycerides of a particular regiospecificity, the natural oil or natural fat has not been subjected to interesterification or other synthetic process to obtain that regiospecific triglyceride profile, rather the regiospecificity is produced naturally, by a cell or population of cells. In connection with a natural oil or natural fat, and as used generally throughout the present disclosure, the terms oil and fat are used interchangeably, except where otherwise noted. Thus, an “oil” or a “fat” can be liquid, solid, or partially solid at room temperature, depending on the makeup of the substance and other conditions.

Here, the term “fractionation” means removing material from the oil in a way that

2018267601 21 Nov 2018 changes its fatty acid profile relative to the profile produced by the organism, however accomplished. The terms “natural oil” and “natural fat” encompass such oils obtained from an organism, where the oil has undergone minimal processing, including refining, bleaching and/or degumming, which does not substantially change its triglyceride profile. A natural oil can also be a “noninteresterified natural oil”, which means that the natural oil has not undergone a process in which fatty acids have been redistributed in their acyl linkages to glycerol and remain essentially in the same configuration as when recovered from the organism.

[0032] “Exogenous gene” shall mean a nucleic acid that codes for the expression of an RNA and/or protein that has been introduced into a cell (e.g. by transformation/transfection), and is also referred to as a “transgene”. A cell comprising an exogenous gene may be referred to as a recombinant cell, into which additional exogenous gene(s) may be introduced. The exogenous gene may be from a different species (and so heterologous), or from the same species (and so homologous), relative to the cell being transformed. Thus, an exogenous gene can include a homologous gene that occupies a different location in the genome of the cell or is under different control, relative to the endogenous copy of the gene. An exogenous gene may be present in more than one copy in the cell. An exogenous gene may be maintained in a cell, for example, as an insertion into the genome (nuclear or plastid) or as an episomal molecule.

[0033] “Fatty acids” shall mean free fatty acids, fatty acid salts, or fatty acyl moieties in a glycerolipid. It will be understood that fatty acyl groups of glycerolipids can be described in terms of the carboxylic acid or anion of a carboxylic acid that is produced when the triglyceride is hydrolyzed or saponified.

[0034] “Microalgae” are microbial organisms that contain a chloroplast or other plastid, and optionally that are capable of performing photosynthesis, or a prokaryotic microbial organism capable of performing photosynthesis. Microalgae include obligate photoautotrophs, which cannot metabolize a fixed carbon source as energy, as well as heterotrophs, which can live solely off of a fixed carbon source.

Microalgae include unicellular organisms that separate from sister cells shortly after cell division, such as Chlamydomonas, as well as microbes such as, for example,

Volvox, which is a simple multicellular photosynthetic microbe of two distinct cell types. Microalgae include cells such as Chlorella, Dunaliella, and Prototheca.

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Microalgae also include other microbial photosynthetic organisms that exhibit cellcell adhesion, such as Agmenellum, Anabaena, and Pyrobotrys. Microalgae also include obligate heterotrophic microorganisms that have lost the ability to perform photosynthesis, such as certain dinoflagellate algae species and species of the genus

Prototheca.

[0035] An “oleaginous” cell is a cell capable of producing at least 20% lipid by dry cell weight, naturally or through recombinant or classical strain improvement. An “oleaginous microbe” or “oleaginous microorganism” is a microbe, including a microalga that is oleaginous.

[0036] The term “percent sequence identity,” in the context of two or more amino acid or nucleic acid sequences, refers to two or more sequences or subsequences that are the same or have a specified percentage of amino acid residues or nucleotides that are the same, when compared and aligned for maximum correspondence, as measured using a sequence comparison algorithm or by visual inspection. For sequence comparison to determine percent nucleotide or amino acid identity, typically one sequence acts as a reference sequence, to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are input into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. The sequence comparison algorithm then calculates the percent sequence identity for the test sequence(s) relative to the reference sequence, based on the designated program parameters. Optimal alignment of sequences for comparison can be conducted using the NCBI BLAST software (ncbi.nlm.nih.gov/BLAST/) set to default parameters. For example, to compare two nucleic acid sequences, one may use blastn with the

BLAST 2 Sequences tool Version 2.0.12 (Apr. 21, 2000) set at the following default parameters: Matrix: BLOSUM62; Reward for match: 1; Penalty for mismatch: -2; Open Gap: 5 and Extension Gap: 2 penalties; Gap x drop-off: 50; Expect: 10; Word Size: 11; Filter: on. For a pairwise comparison of two amino acid sequences, one may use the “BLAST 2 Sequences” tool Version 2.0.12 (Apr. 21,

2000) with blastp set, for example, at the following default parameters: Matrix:

BLOSUM62; Open Gap: 11 and Extension Gap: 1 penalties; Gap x drop-off 50; Expect: 10; Word Size: 3; Filter: on.

2018267601 21 Nov 2018 [0037] In connection with a natural oil, a “profile” is the distribution of particular species or triglycerides or fatty acyl groups within the oil. A “fatty acid profile” is the distribution of fatty acyl groups in the triglycerides of the oil without reference to attachment to a glycerol backbone. Fatty acid profiles are typically determined by conversion to a fatty acid methyl ester (FAME), followed by gas chromatography (GC) analysis with flame ionization detection (FID). The fatty acid profile can be expressed as one or more percent of a fatty acid in the total fatty acid signal determined from the area under the curve for that fatty acid. FAME-GC-FID measurement approximate weight percentages of the fatty acids.

[0038] As used herein, an oil is said to be “enriched” in one or more particular fatty acids if there is at least a 10% increase in the mass of that fatty acid in the oil relative to the non-enriched oil. For example, in the case of a cell expressing a heterologous FatB gene described herein, the oil produced by the cell is said to be enriched in, e.g., C8 and C16 fatty acids if the mass of these fatty acids in the oil is at least 10% greater than in oil produced by a cell of the same type that does not express the heterologous FatB gene (e.g., wild type oil).

[0039] ‘ ‘Recombinant” is a cell, nucleic acid, protein or vector that has been modified due to the introduction of an exogenous nucleic acid or the alteration of a native nucleic acid. Thus, e.g., recombinant (host) cells can express genes that are not 20 found within the native (non-recombinant) form of the cell or express native genes differently than those genes are expressed by a non-recombinant cell. Recombinant cells can, without limitation, include recombinant nucleic acids that encode a gene product or suppression elements such as mutations, knockouts, antisense, interfering RNA (RNAi) or dsRNA that reduce the levels of active gene product in a cell. A “recombinant nucleic acid” is a nucleic acid originally formed in vitro, in general, by the manipulation of nucleic acid, e.g., using polymerases, ligases, exonucleases, and endonucleases, using chemical synthesis, or otherwise is in a form not normally found in nature. Recombinant nucleic acids may be produced, for example, to place two or more nucleic acids in operable linkage. Thus, an isolated nucleic acid or an expression vector formed in vitro by nucleic by ligating DNA molecules that are not normally joined in nature, are both considered recombinant for the purposes of this invention. Recombinant nucleic acids can also be produced in other ways; e.g., using chemical DNA synthesis. Once a recombinant nucleic acid is made and introduced

2018267601 21 Nov 2018 into a host cell or organism, it may replicate using the in vivo cellular machinery of the host cell; however, such nucleic acids, once produced recombinantly, although subsequently replicated intracellularly, are still considered recombinant for purposes of this invention. Similarly, a “recombinant protein” is a protein made using recombinant techniques, i.e., through the expression of a recombinant nucleic acid. [0040] Embodiments of the present invention relate to the use of FatB genes isolated from plants, which can be expressed in a host cell in order to alter the fatty acid profile of an oil produced by the recombinant cell. Although the microalga, Prototheca moriformis, was used to screen the genes for ability to the alter fatty acid profile, the genes are useful in a wide variety of host cells. For example, the genes can be expressed in bacteria, other microalgae, or higher plants. The genes can be expressed in higher plants according to the methods of US Patent Nos. 5,850,022; 5,723,761; 5,639,790; 5,807,893; 5,455,167; 5,654,495;

5,512,482;5,298,421;5,667,997; 5,344,771; and 5,304,481. The fatty acids can be further converted to triglycerides, fatty aldehydes, fatty alcohols and other oleochemicals either synthetically or biosynthetically.

[0041] In specific embodiments, triglycerides are produced by a host cell expressing a novel FatB gene. A triglyceride-containing natural oil can be recovered from the host cell. The natural oil can be refined, degummed, bleached and/or deodorized. The oil, in its natural or processed form, can be used for foods, chemicals, fuels, cosmetics, plastics, and other uses. In other embodiments, the FatB gene may not be novel, but the expression of the gene in a microalga is novel.

[0042] The genes can be used in a variety of genetic constructs including plasmids or other vectors for expression or recombination in a host cell. The genes can be codon optimized for expression in a target host cell. The proteins produced by the genes can be used in vivo or in purified form.

[0043] For example, the gene can be prepared in an expression vector comprising an operably linked promoter and 5 ’UTR. Where a plastidic cell is used as the host, a suitably active plastid targeting peptide can be fused to the FATB gene, 30 as in the examples below. Generally, for the newly identified FATB genes, there are roughly 50 amino acids at the N-terminal that constitute a plastid transit peptide, which are responsible for transporting the enzyme to the chloroplast. In the examples below, this transit peptide is replaced with a 38 amino acid sequence that is effective

2018267601 21 Nov 2018 in the Prototheca moriformis host cell for transporting the enzyme to the plastids of those cells. Thus, the invention contemplates deletions and fusion proteins in order to optimize enzyme activity in a given host cell. For example, a transit peptide from the host or related species may be used instead of that of the newly discovered plant genes described here.

[0044] A selectable marker gene may be included in the vector to assist in isolating a transformed cell. Examples of selectable markers useful in microlagae include sucrose invertase and antibiotic resistance genes.

[0045] The gene sequences disclosed can also be used to prepare antisense, or inhibitory RNA (e.g., RNAi or hairpin RNA) to inhibit complementary genes in a plant or other organism.

[0046] FatB genes found to be useful in producing desired fatty acid profiles in a cell are summarized below in Table 1. Nucleic acids or proteins having the sequence of SEQ ID NOS: 1-109 can be used to alter the fatty acid profile of a recombinant cell. Variant nucleic acids can also be used; e.g., variants having at least 70, 80, 85, 90, 95, 96, 97, 98, or 99% sequence identity to SEQ ID NOS: 2, 3, 5, 6, 8, 9, 11, 12, 14, 15, 17, 18, 20, 21, 23, 24, 26, 27, 29, 30, 32, 33, 35, 36, 38, 39, 41, 42, 44, 45, 47, 48, 50, 51, 53, 54, 56, 57, 59, 60, 62, 63, 65, 66, 68, 69, 71, 72, 74, 76, 78, 80, 81, 83, 84, 86, 87, 89, 90, 92, 93, 95, 96, 98, 99, 101, 102, 104, 105, 107 or 109.

Codon optimization of the genes for a variety of host organisms is contemplated, as is the use of gene fragments. Preferred codons for Prototheca strains and for Chlorella protothecoides are shown below in Tables 2 and 3, respectively. Codon usage for Cuphea wrightii is shown in Table 3a. Codon usage for Arabidopsis is shown in Table 3b; for example, the most preferred of codon for each amino acid can be selected. Codon tables for other organisms including microalgae and higher plants are known in the art. In some embodiments, the first and/or second most preferred Prototheca codons are employed for codon optimization. In specific embodiments, the novel amino acid sequences contained in the sequence listings below are converted into nucleic acid sequences according to the most preferred codon usage in

Prototheca, Chlorella, Cuphea wrightii, or Arabidopsis as set forth in tables 2 through

3b or nucleic acid sequences having at least 70, 80, 85, 90, 95, 96, 97, 98, or 99% sequence identity to these derived nucleic acid sequences.

2018267601 21 Nov 2018 [0047] In embodiments of the invention, there is protein or a nucleic acid encoding a protein having any of SEQ ID NOS: 1,4,7, 10, 13, 16, 19, 22, 25,28,31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 75, 77, 79, 82, 85, 88, 91, 94, 97, 100, 103, 106, 108, or 110-192. In an embodiment, there is protein or a nucleic acid encoding a protein having at least 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or 100% sequence identity with any of SEQ ID NOS: 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 75, 77, 79, 82, 85, 88, 91, 94, 97, 100, 103, 106, 108, or 110-192. In certain embodiments, the invention encompasses a fragment any of the above-described proteins or nucleic acids (including fragments of protein or nucleic acid variants), wherein the protein fragment has acyl-ACP thioesterase activity or the nucleic acid fragment encodes such a protein fragment. In other embodiments, the fragment includes a domain of an acyl-ACP thioesterase that mediates a particular function, e.g., a specificity-determining domain. Illustrative fragments can be produced by C-terminal and/or N-terminal truncations and include at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% of the full-length sequences disclosed herein.

[0048] In certain embodiments, percent sequence identity for variants of the nucleic acids or proteins discussed above can be calculated by using the full-length nucleic acid sequence (e.g., one of SEQ ID NOS: 2, 3, 5, 6, 8, 9, 11, 12, 14, 15, 17,

18, 20, 21, 23, 24, 26, 27, 29, 30, 32, 33, 35, 36, 38, 39, 41, 42, 44, 45, 47, 48, 50, 51,

53, 54, 56, 57, 59, 60, 62, 63, 65, 66, 68, 69, 71, 72, 74, 76, 78, 80, 81, 83, 84, 86, 87, 89, 90, 92, 93, 95, 96, 98, 99, 101, 102, 104, 105, 107 or 109) or full-length amino acid sequence (e.g., one of SEQ ID NOS: 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 75, 77, 79, 82, 85, 88, 91, 94, 97, 100,

103, 106, 108, or 110-192) as the reference sequence and comparing the full-length test sequence to this reference sequence. In some embodiments relating to fragments, percent sequence identity for variants of nucleic acid or protein fragments can be calculated over the entire length of the fragment.

[0049] The nucleic acids can be in isolated form, or part of a vector or other construct, chromosome or host cell. It has been found that is many cases the full length gene (and protein) is not needed; for example, deletion of some or all of the Nterminal hydrophobic domain (typically an 18 amino acid domain starting with LPDW) yields a still-functional gene. In addition, fusions of the specificity

2018267601 21 Nov 2018 determining regions of the genes in Table 1 with catalytic domains of other acyl-ACP thioesterases can yield functional genes. Thus, in certain embodiments, the invention encompasses functional fragments (e.g., specificity determining regions) of the disclosed nucleic acid or amino acids fused to heterologous acyl-ACP thioesterase nucleic acid or amino acid sequences, respectively.

Table 1: FatB genes according to embodiments of the present invention

Species	Gene Name	Sequence Variant (relative to dominant transcript identified)	Amino Acid Sequenc e of CDS (no additiona 1 tags)	Native CDS nucleotid e sequence (not codonoptimize d, no additiona 1 cloning sites)	Protothec a moriformi s codonoptimized nucleotid e sequence of CDS
Cinnamomu m camphora	CcFATBlb	M25L, M322R, AT367-D368	SEQ ID NO: 1	SEQ ID NO: 2	SEQ ID NO: 3
Cinnamomu m camphora	CcFATB4	wild-type	SEQ ID NO: 4	SEQ ID NO: 5	SEQ ID NO: 6
Cinnamomu m camphora	CcFATB3	wild-type	SEQ ID NO: 7	SEQ ID NO: 8	SEQ ID NO: 9
Cuphea hyssopifolia	ChsFATBl	wild-type	SEQ ID NO: 10	SEQ ID NO: 11	SEQ ID NO: 12
Cuphea hyssopifolia	ChsFATB2	wild-type	SEQ ID NO: 13	SEQ ID NO: 14	SEQ ID NO: 15

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Cuphea hyssopifolia	ChsFATB2b	+a.a.248-259	SEQ ID NO: 16	SEQ ID NO: 17	SEQ ID NO: 18
Cuphea hyssopifolia	ChsFATB3	wild-type	SEQ ID NO: 19	SEQ ID NO: 20	SEQ ID NO: 21
Cuphea hyssopifolia	ChsFATB3b	V204I,C239F, E243D, M251V	SEQ ID NO: 22	SEQ ID NO: 23	SEQ ID NO: 24
Cuphea PSR23	CuPSR23FATB 3	wild-type	SEQ ID NO: 25	SEQ ID NO: 26	SEQ ID NO: 27
Cuphea wrightii	CwFATB3	wild-type	SEQ ID NO: 28	SEQ ID NO: 29	SEQ ID NO: 30
Cuphea wrightii	CwFATB4a	wild-type	SEQ ID NO: 31	SEQ ID NO: 32	SEQ ID NO: 33
Cuphea wrightii	CwFATB4b	wild-type	SEQ ID NO: 34	SEQ ID NO: 35	SEQ ID NO: 36
Cuphea wrightii	CwFATB5	wild-type	SEQ ID NO: 37	SEQ ID NO: 38	SEQ ID NO: 39
Cuphea heterophylla	ChtFATBla	wild-type	SEQ ID NO: 40	SEQ ID NO: 41	SEQ ID NO: 42
Cuphea heterophylla	ChtFATBlb	P16S, T20P, G94S, G105W, S293F, L305F	SEQ ID NO: 43	SEQ ID NO: 44	SEQ ID NO: 45
Cuphea heterophylla	ChtFATB2b	wild-type	SEQ ID NO: 46	SEQ ID NO: 47	SEQ ID NO: 48
Cuphea heterophylla	ChtFATB2a	S17P, P21S, T28N, L30P, S33L, G76D, S78P, G137W	SEQ IDO NO: 49	SEQ ID NO: 50	SEQ ID NO: 51
Cuphea heterophylla	ChtFATB2c	G76D, S78P	SEQ ID NO: 52	SEQ ID NO: 53	SEQ ID NO: 54
Cuphea heterophylla	ChtFATB2d	S21P, T28N, L30P, S33L, G76D, R97L, H124L, W127L,	SEQ ID NO: 55	SEQ ID NO: 56	SEQ ID NO: 57

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		I132S, K258N, C303R, E309G, K334T, T386A
Cuphea heterophylla	ChtFATB2e	G76D, R97L, H124L, I132S, G152S, H165L, T211N, K258N, C303R, E309G, K334T, T386A	SEQ ID NO: 58	SEQ ID NO: 59	SEQ ID NO: 60
Cuphea heterophylla	ChtFATB2f	R97L, H124L, I132S, G152S, H165L, T211N	SEQ ID NO: 61	SEQ ID NO: 62	SEQ ID NO: 63
Cuphea heterophylla	ChtFATB2g	A6T, A16V, S17P, G76D, R97L, H124L, I132S, S143I, G152S, A157T, H165L, T211N, G414A	SEQ ID NO: 64	SEQ ID NO: 65	SEQ ID NO: 66
Cuphea heterophylla	ChtFATB3a	wild-type	SEQ ID NO: 67	SEQ ID NO: 68	SEQ ID NO: 69
Cuphea heterophylla	ChtFATB3b	C67G, H72Q, L128F, N179I	SEQ ID NO: 70	SEQ ID NO: 71	SEQ ID NO: 72
Cuphea viscosissima	CvisFATBl	published	SEQ ID NO: 73	N/A	SEQ ID NO: 74
Cuphea viscosissima	CvisFATB2	published	SEQ ID NO: 75	N/A	SEQ ID NO: 76
Cuphea viscosissima	CvisFATB3	published	SEQ ID NO: 77	N/A	SEQ ID NO: 78
Cuphea calcarata	CcalcFATBl	wild-type	SEQ ID NO: 79	SEQ ID 80	SEQ ID 81
Cuphea painteri	CpaiFATBl	wild-type	SEQ ID NO: 82	SEQ ID 83	SEQ ID 84

2018267601 21 Nov 2018

Cuphea hookeriana	ChookFATB4	wild-type	SEQ ID NO: 85	SEQ ID 86	SEQ ID 87
Cuphea avigera var. pulcherrima	CaFATBl	wild-type	SEQ ID NO: 88	SEQ ID 89	SEQ ID 90
Cuphea paucipetala	CPauFATBl	wild-type	SEQ ID NO: 91	SEQ ID 92	SEQ ID 93
Cuphea procumbens	CprocFATBl	wild-type	SEQ ID NO: 94	SEQ ID 95	SEQ ID 96
Cuphea procumbens	CprocFATB2	wild-type	SEQ ID NO: 97	SEQ ID 98	SEQ ID 99
Cuphea procumbens	CprocFATB3	wild-type	SEQ ID NO: 100	SEQ ID 101	SEQ ID 102
Cuphea ignea	CigneaFATB 1	wildtype; partial (missing Nterminal portion of native transit peptide, fused to CpSADltp trimm ed transit peptide)	SEQ ID NO: 103	SEQ ID 104	SEQ ID 105
Consensus	JcFATBl	Consensus sequence	SEQ ID NO: 106	None, can be codon optimize d for a given	SEQ ID NO: 107

2018267601 21 Nov 2018

				host
Consensus	JcFATB2	Consensus sequence	SEQ ID NO: 108	None, can be codon optimize d for a given host	SEQ ID NO: 109

In certain embodiments, a host cell (e.g. plant or microalgal cell) is transformed to produce a recombinant FATB protein falling into one of clades 1-12 of Table la. These clades were determined by sequence alignment and observation of changes in 5 fatty acid profile when expressed in Prototheca. See Example 5. The FATB amino acid sequence can fall within x% amino acid sequence identity of each sequence in that clade listed in Table la, where x is a first second or third cutoff value, also listed in Table la.

2018267601 21 Nov 2018

Table la: Groupings of Novel FatB genes into clades.

Clade No.	Amino Acid SEQ ID Nos. in Clade	Example Function (see Table 6)	First Cutoff Value (minimum % amino acid identity to members of clade)	Second Cutoff Value	Third Cutoff Value
1	ChsFATB3 (SEQ ID NO: 19) ChsFATB3d (SEQ ID NO: 161) ChsFATB3b (SEQ ID NO: 22) ChsFATB3c (SEQ ID NO: 160)	Increase Cl 4/C 16 fatty acids	85.9	97.4	98
2	ChtFATBla.2 (SEQ ID NO: 134) ChtFATBla.3 (SEQ ID NO: 135) ChtFATBla.4 (SEQ ID NO: 136) ChtFATBla (SEQ ID NO: 132)_ ChtFATBla.l (SEQ ID NO: 133) ChtFATBlb (SEQ ID NO: 137) CwFATB5b (SEQ ID NO: 124) CwFATB5 (SEQ ID NO: 122) CwFATB5a (SEQ ID NO: 123) CwFATB5c (SEQ ID NO: 125)	Increase Cl 2/C 14 fatty acids	89.5	95	98
3	CwFATB5.1 (SEQ ID NO: 126) CwFATB5.1a (SEQ ID NO: 127)	Increase Cl 2/C 14 fatty acids	92.5	95	98
4	CcalcFATBl (SEQ ID NO: 79)	Increase Cl 2/C 14 fatty acids	83.8	93	95
5	CaFATBl (SEQ ID NO: 88) CpaiFATBl (SEQ ID NO: 82) ChookFATB4 (SEQ ID NO: 85) CigneaFATBl (SEQ ID NO: 103)	Increase C8/C10 fatty acids	94.6	96	98
6	CuPSR23FATB3b (SEQ ID NO: 111) CuPSR23FATB3 (SEQ ID NO: 110)	Increase C10 fatty acids	99.9
7	CvisFATBl (SEQ ID NO: 73) JcFATBl/SzFATBl (SEQ ID NO: 106)	Increase Cl 0/C 12 fatty acids	89.5	93	96

2018267601 21 Nov 2018

	CgFATBlb (SEQ ID NO: 185) CprocFATBl (SEQ ID NO: 172) CpauFATBl (SEQ ID NO: 171) CprocFATB2 (SEQ ID NO: 173) CprocFATB3 (SEQ ID NO: 174)
8	CwFATB3 (SEQ ID NO: 112) CwFATB3a (SEQ ID NO: 113) ChtFATB2e (SEQ ID NO: 142) ChtFATB2h (SEQ ID NO: 145) ChtFATB2f (SEQ ID NO: 143) ChtFATB2g (SEQ ID NO: 144) ChtFATB2a (SEQ ID NO: 139) ChtFATB2c (SEQ ID NO: 140) ChtFATB2b (SEQ ID NO: 138) ChtFATB2d (SEQ ID NO: 141)	Increase Cl 2/C 14 fatty acids	85.9	98.9	99.5
9	CcrFATB2c (SEQ ID NO: 187) CcrFATB2 (SEQ ID NO: 188) CcrFATB2b (SEQ ID NO: 189)	Increase Cl 2/C 14 fatty acids	83.8	90	95
10	ChtFATB3b (SEQ ID NO: 147) ChtFATB3d (SEQ ID NO: 149) ChtFATB3a (SEQ ID NO: 146) ChtFATB3e (SEQ ID NO: 150) ChtFATB3g (SEQ ID NO: 152) ChtFATB3f (SEQ ID NO: 151) ChtFATB3c (SEQ ID NO: 148) ChsFATB2 (SEQ ID NO: 154) ChsFATB2c (SEQ ID NO: 156) ChsFATB2b (SEQ ID NO: 155) ChsFATB2d (SEQ ID NO: 157) JcFATB2/SzFATB2 (SEQ ID NO: 108) CvisFATB2 (SEQ ID NO: 75) CcrFATBl (SEQ ID NO: 190 ) CcrFATBlb (SEQ ID NO: 191) CcrFATBlc (SEQ ID NO: 192 )	Increase Cl 4/C 16 fatty acids	95.9	98	99
11	CwFATB4b.l (SEQ ID NO: 121)	Increase Cl 4/C 16 fatty acids	88.7	94.5	97
12	CcFATB3 (SEQ ID NO: 129) UcFATB3 (SEQ ID NO: 186) (predicted)	Increase C16 fatty acids	72.8	85	90

2018267601 21 Nov 2018

Table 2: Preferred codon usage in Prototheca strains

Ala	GCG	345 (0.36)	Asn	AAT	8 (0.04)
	GCA	66 (0.07)		AAC	201 (0.96)
	GCT	101 (0.11)
	GCC	442 (0.46)	Pro	CCG	161 (0.29)
				CCA	49 (0.09)
Cys	TGT	12(0.10)		CCT	71 (0.13)
	TGC	105 (0.90)		CCC	267 (0.49)
Asp	GAT	43 (0.12)	Gin	CAG	226 (0.82)
	GAC	316(0.88)		CAA	48 (0.18)
Glu	GAG	377 (0.96)	Arg	AGG	33 (0.06)
	GAA	14 (0.04)		AGA	14 (0.02)
				CGG	102 (0.18)
Phe	TTT	89 (0.29)		CGA	49 (0.08)
	TTC	216(0.71)		CGT	51 (0.09)
				CGC	331 (0.57)
Gly	GGG	92 (0.12)
	GGA	56 (0.07)	Ser	AGT	16 (0.03)
	GGT	76 (0.10)		AGC	123 (0.22)
	GGC	559(0.71)		TCG	152 (0.28)
				TCA	31 (0.06)

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His	CAT	42 (0.21)	TCT	55 (0.10)
	CAC	154 (0.79)	TCC	173 (0.31)

Ile	ATA	4(0.01)	Thr	ACG	184 (0.38)
5		ATT	30 (0.08)		ACA	24 (0.05)
		ATC	338 (0.91)		ACT	21 (0.05)
					ACC	249 (0.52)
	Lys	AAG	284 (0.98)
		AAA	7 (0.02)	Val	GTG	308 (0.50)
10					GTA	9(0.01)
	Leu	TTG	26 (0.04)		GTT	35 (0.06)
		TTA	3 (0.00)		GTC	262 (0.43)
		CTG	447 (0.61)
		CTA	20 (0.03)	Trp	TGG	107(1.00)
15		CTT	45 (0.06)
		CTC	190 (0.26)	Tyr	TAT	10 (0.05)

TAC 180 (0.95)

Met ATG 191 (1.00)

Stop TGA/TAG/TAA

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Table 3: Preferred codon usage in Chlorella protothecoides
TTC (Phe)	TAC (Tyr)	TGC (Cys)	TGA (Stop)
TGG (Trp)	CCC (Pro)	CAC (His)	CGC (Arg)
CTG (Leu)	CAG (Gin)	ATC (Ile)	ACC (Thr)
5 GAC (Asp)	TCC (Ser)	ATG (Met)	AAG (Lys)
GCC (Ala)	AAC (Asn)	GGC (Gly)	GTG (Val)

GAG (Glu)

Table 3a: Codon usage for Cuphea wrightii

UUU F 0.48 19.5 (

10.5( 28)

UUCF 0.52 21.3 (

15.0( 40)

UUAL0.07 5.2 (

2)

UUGL0.19 14.6( ( 41)

52) UCUS0.21 19.5(

57) UCC S 0.26 23.6 (

14) UCAS0.18 16.8(

39) UCGS0.11 9.7(

52) UAU Y 0.45 6.4 (

63) UAC Y 0.55 7.9 (

45) UAA * 0.33 0.7 (

26) UAG * 0.33 0.7 (

17) UGUC0.41

21) UGCC0.59

2) UGA * 0.33 0.7 (

2) UGG W 1.00 15.4

CUUL 0.27 21.0( 20 5.6( 15)

CUC L 0.22 17.2 ( ( 21)

CUAL0.13 10.1 ( ( 18)

CUGL0.12 9.7( ( 25)

56) CCU P 0.48 21.7 (

46) CCCP0.16 7.1 (

27) CCA P 0.21 9.7(

26) CCGP0.16 7.1 (

58) CAUH0.60 11.2(

19) CACH0.40 7.5 (

26) CAAQ0.31 8.6(

19) CAGQ0.69 19.5(

30) CGUR0.09

20) CGCR0.13 7.9

23) CGAR0.11 6.7

52) CGGR0.16 9.4

2018267601 21 Nov 2018

AUU I 0.44 22.8 (

16.1 ( 43)

AUC I 0.29 15.4( ( 16)

AUAI0.27 13.9( 14.2( 38)

AUG M 1.00 28.1 (

16.1 ( 43)

GUUV0.28 19.8 (

26.6( 71)

GUCV0.21 15.0(

18.0( 48)

GUA V 0.14 10.1 (

31.4( 84)

GUGV 0.36 25.1 (

14.2( 38)

61) ACUT0.33 16.8 (

41) ACCT 0.27 13.9(

37) ACAT0.26 13.5 (

75) ACGT0.14 7.1 (

53) GCU A 0.35 31.4 (

40) GCCA0.20 18.0(

27) GCA A 0.33 29.6 (

67) GCGA0.11 9.7(

45) AAUN 0.66 31.4 (

37) AACN0.34 16.5(

36) AAA K 0.42 21.0 (

19) AAG K 0.58 29.2 (

84) GAU D 0.63 35.9 (

48) GACD 0.37 21.0 (

79) GAAE0.41 18.3 (

26) GAG E 0.59 26.2 (

84) AGUS0.18

44) AGC S 0.07 6.0

56) AGAR 0.24

78) AGGR0.27

96) GGUG0.29

56) GGCG0.20

49) GGAG0.35

70) GGGG0.16

Table 3b: Codon usage for Arabidopsis

UUUF 0.51 21.8 (678320) UCU S 0.28 25.2 (782818) UAUY 0.52 14.6 (455089) UGU C

0.60 10.5 (327640)

UUC F 0.49 20.7 (642407) UCCS 0.13 11.2 (348173) UAC Y 0.48 13.7(427132) UGC C

0.40 7.2(222769)

UUAL 0.14 12.7(394867) UCA S 0.20 18.3 (568570) UAA * 0.36 0.9(29405) UGA *

0.44 1.2(36260)

UUGL 0.22 20.9 (649150) UCGS 0.10 9.3 (290158) UAG * 0.20 0.5 ( 16417) UGG W

1.00 12.5 (388049)

2018267601 21 Nov 2018

CUUL 0.26 24.1 (750114) CCU P 0.38 18.7 (580962) CAUH0.61 13.8 (428694) CGUR

0.17 9.0(280392)

CUC L 0.17 16.1 (500524) CCCP0.11 5.3 (165252) CAC H 0.39 8.7 (271155) CGC R

0.07 3.8 (117543)

CUAL0.11 9.9 (307000) CCA P 0.33 16.1 (502101) CAA Q 0.56 19.4 (604800) CGAR

0.12 6.3 (195736)

CUGL 0.11 9.8 (305822) CCGP 0.18 8.6 (268115) CAG Q 0.44 15.2 (473809) CGG R

0.09 4.9(151572)

AUU I 0.41 21.5 (668227) ACU T 0.34 17.5 (544807) AAU N 0.52 22.3 (693344) AGU S

0.16 14.0 (435738)

AUC I 0.35 18.5 (576287) ACC T 0.20 10.3 (321640) AAC N 0.48 20.9 (650826) AGC S

0.13 11.3 (352568)

AUA I 0.24 12.6(391867) ACAT0.31 15.7 (487161) AAA K 0.49 30.8 (957374) AGAR

0.35 19.0 (589788)

AUG M 1.00 24.5 (762852) ACGT0.15 7.7 (240652) AAG K 0.51 32.7 (1016176) AGG

R0.20 11.0 (340922)

GUUV 0.40 27.2 (847061) GCU A 0.43 28.3 (880808) GAU D 0.68 36.6 (1139637) GGU

G 0.34 22.2 (689891)

GUCV 0.19 12.8 (397008) GCC A 0.16 10.3 (321500) GAC D 0.32 17.2 (535668) GGC G

0.14 9.2(284681)

GUAV0.15 9.9 (308605) GCAA0.27 17.5 (543180) GAA E 0.52 34.3 (1068012) GGA

G 0.37 24.2 (751489)

GUG V 0.26 17.4 (539873) GCG A 0.14 9.0 (280804) GAG E 0.48 32.2 (1002594) GGG

G0.16 10.2 (316620)

2018267601 21 Nov 2018

Host Cells [0050] The host cell can be a single cell (e.g., microalga, bacteria, yeast) or part of a multicellular organism such as a plant or fungus. Methods for expressing Fatb genes in a plant are given in 5,850,022; 5,723,761; 5,639,790; 5,807,893;

5,455,167; 5,654,495; 5,512,482;5,298,421;5,667,997; and 5,344,771; 5,304,481, or can be accomplished using other techniques generally known in plant biotechnology. Engineering of oleaginous microbes including those of Chlorophyta is disclosed in W02010/063032, WO2011,150411, and W02012/106560 and in the examples below.

[0051] Examples of oleaginous host cells include plant cells and microbial cells having a type II fatty acid biosynthetic pathway, including plastidic oleaginous cells such as those of oleaginous algae. Specific examples of microalgal cells include heterotrophic or obligate heterotrophic microalgae of the phylum Chlorophtya, the class Trebouxiophytae, the order Chlorellales, or the family Chlorellacae. Examples of oleaginous microalgae are provided in Published PCT Patent Applications

W02008/151149, WO2010/06032, WO2011/150410, and WO2011/150411, including species of Chlorella and Prototheca, a genus comprising obligate heterotrophs. The oleaginous cells can be, for example, capable of producing 25, 30, 40, 50, 60, 70, 80, 85, or about 90% oil by cell weight, ±5%. Optionally, the oils produced can be low in DHA or EPA fatty acids. For example, the oils can comprise less than 5%, 2 %, or 1% DHA and/or EPA. The above-mentioned publications also disclose methods for cultivating such cells and extracting oil, especially from microalgal cells; such methods are applicable to the cells disclosed herein and incorporated by reference for these teachings. When microalgal cells are used they can be cultivated autotrophically (unless an obligate heterotroph) or in the dark using a sugar (e.g., glucose, fructose and/or sucrose). In any of the embodiments described herein, the cells can be heterotrophic cells comprising an exogenous invertase gene so as to allow the cells to produce oil from a sucrose feedstock. Alternately, or in addition, the cells can metabolize xylose from cellulosic feedstocks. For example, the cells can be genetically engineered to express one or more xylose metabolism genes such as those encoding an active xylose transporter, a xylulose-5-phosphate transporter, a xylose isomerase, a xylulokinase, a xylitol dehydrogenase and a xylose

2018267601 21 Nov 2018 reductase. See WO2012/154626, “GENETICALLY ENGINEERED MICROORGANISMS THAT METABOLIZE XYLOSE”, published Nov 15, 2012.

Oils and Related Products [0052] The oleaginous cells express one or more exogenous genes encoding fatty acid biosynthesis enzymes. As a result, some embodiments feature natural oils that were not obtainable from a non-plant or non-seed oil, or not obtainable at all.

[0053] The oleaginous cells produce a storage oil, which is primarily triacylglyceride and may be stored in storage bodies of the cell. A raw oil may be obtained from the cells by disrupting the cells and isolating the oil. W02008/151149,

W02010/06032, WO2011/150410, and WO2011/1504 disclose heterotrophic cultivation and oil isolation techniques. For example, oil may be obtained by cultivating, drying and pressing the cells. The oils produced may be refined, bleached and deodorized (RBD) as known in the art or as described in W02010/120939. The raw or RBD oils may be used in a variety of food, chemical, and industrial products or processes. After recovery of the oil, a valuable residual biomass remains. Uses for the residual biomass include the production of paper, plastics, absorbents, adsorbents, as animal feed, for human nutrition, or for fertilizer.

[0054] Where a fatty acid profile of a triglyceride (also referred to as a “triacylglyceride” or “TAG”) cell oil is given here, it will be understood that this refers to a nonfractionated sample of the storage oil extracted from the cell analyzed under conditions in which phospholipids have been removed or with an analysis method that is substantially insensitive to the fatty acids of the phospholipids (e.g. using chromatography and mass spectrometry). The oil may be subjected to an RBD process to remove phospholipids, free fatty acids and odors yet have only minor or negligible changes to the fatty acid profile of the triglycerides in the oil. Because the cells are oleaginous, in some cases the storage oil will constitute the bulk of all the TAGs in the cell.

[0055] The stable carbon isotope value 613C is an expression of the ratio of

13C/12C relative to a standard (e.g. PDB, carbonite of fossil skeleton of Belemnite americana from Peedee formation of South Carolina). The stable carbon isotope value 613C (0/00) of the oils can be related to the 613C value of the feedstock used.

2018267601 21 Nov 2018

In some embodiments, the oils are derived from oleaginous organisms heterotrophically grown on sugar derived from a C4 plant such as com or sugarcane.

In some embodiments the 613C (0/00) of the oil is from -10 to -17 0/00 or from -13 to -16 0/00.

[0056] The oils produced according to the above methods in some cases are made using a microalgal host cell. As described above, the microalga can be, without limitation, fall in the classification of Chlorophyta, Trebouxiophyceae , Chlorellales, Chlorellaceae, or Chlorophyceae. It has been found that microalgae of Trebouxiophyceae can be distinguished from vegetable oils based on their sterol profiles. Oil produced by Chlorella protothecoides was found to produce sterols that appeared to be brassicasterol, ergosterol, campesterol, stigmasterol, and β-sitosterol, when detected by GC-MS. However, it is believed that all sterols produced by Chlorella have Ο24β stereochemistry. Thus, it is believed that the molecules detected as campesterol, stigmasterol, and β-sitosterol, are actually 22,2315 dihydrobrassicasterol, proferasterol and clionasterol, respectively. Thus, the oils produced by the microalgae described above can be distinguished from plant oils by the presence of sterols with Ο24β stereochemistry and the absence of C24a stereochemistry in the sterols present. For example, the oils produced may contain 22, 23-dihydrobrassicasterol while lacking campesterol; contain clionasterol, while lacking in β-sitosterol, and/or contain poriferasterol while lacking stigmasterol. Alternately, or in addition, the oils may contain significant amounts of Δ⁷poriferasterol.

[0057] In one embodiment, the oils provided herein are not vegetable oils. Vegetable oils are oils extracted from plants and plant seeds. Vegetable oils can be distinguished from the non-plant oils provided herein on the basis of their oil content. A variety of methods for analyzing the oil content can be employed to determine the source of the oil or whether adulteration of an oil provided herein with an oil of a different (e.g. plant) origin has occurred. The determination can be made on the basis of one or a combination of the analytical methods. These tests include but are not limited to analysis of one or more of free fatty acids, fatty acid profile, total triacylglycerol content, diacylglycerol content, peroxide values, spectroscopic properties (e.g. UV absorption), sterol profile, sterol degradation products, antioxidants (e.g. tocopherols), pigments (e.g. chlorophyll), dl3C values and sensory

2018267601 21 Nov 2018 analysis (e.g. taste, odor, and mouth feel). Many such tests have been standardized for commercial oils such as the Codex Alimentarius standards for edible fats and oils. [0058] Sterol profile analysis is a particularly well-known method for determining the biological source of organic matter. Campesterol, b-sitosterol, and stigamsterol are common plant sterols, with b-sitosterol being a principle plant sterol. For example, b-sitosterol was found to be in greatest abundance in an analysis of certain seed oils, approximately 64% in com, 29% in rapeseed, 64% in sunflower, 74% in cottonseed, 26% in soybean, and 79% in olive oil (Gul et al. J. Cell and Molecular Biology 5:71-79, 2006).

[0059] Oil isolated from Prototheca moriformis strain UTEX1435 were separately clarified (CL), refined and bleached (RB), or refined, bleached and deodorized (RBD) and were tested for sterol content according to the procedure described in JAOCS vol.

60, no.8, August 1983. Results of the analysis are shown below (units in mg/lOOg):

	Sterol	Crude	Clarified	Refined & bleached	Refined, bleached, & deodorized
1	Ergosterol	384 (56%)	398 (55%)	293 (50%)	302 (50%)
2	5,22-cholestadien-24methyl-3-ol (Brassicasterol)	14.6 (2.1%)	18.8 (2.6%)	14 (2.4%)	15.2 (2.5%)
3	24-methylcholest-5en-3-ol (Campesterol or 22,23dihydrobrassicasterol)	10.7 (1.6%)	11.9 (1.6%)	10.9 (1.8%)	10.8 (1.8%)
4	5,22-cholestadien-24ethyl-3-ol (Stigmasterol or poriferasterol)	57.7 (8.4%)	59.2 (8.2%)	46.8 (7.9%)	49.9 (8.3%)
5	24-ethylcholest-5-en- 3-ol (β-Sitosterol or clionasterol)	9.64 (1.4%)	9.92 (1.4%)	9.26 (1.6%)	10.2 (1.7%)
6	Other sterols	209	221	216	213
	Total sterols	685.64	718.82	589.96	601.1

[0060] These results show three striking features. First, ergosterol was found to be the most abundant of all the sterols, accounting for about 50% or more of the total

2018267601 21 Nov 2018 sterols. The amount of ergosterol is greater than that of campesterol, β-sitosterol, and stigmasterol combined. Ergosterol is steroid commonly found in fungus and not commonly found in plants, and its presence particularly in significant amounts serves as a useful marker for non-plant oils. Secondly, the oil was found to contain brassicasterol. With the exception of rapeseed oil, brassicasterol is not commonly found in plant based oils. Thirdly, less than 2% β-sitosterol was found to be present. β-sitosterol is a prominent plant sterol not commonly found in microalgae, and its presence particularly in significant amounts serves as a useful marker for oils of plant origin. In summary, Prototheca moriformis strain UTEX1435 has been found to contain both significant amounts of ergosterol and only trace amounts of β-sitosterol as a percentage of total sterol content. Accordingly, the ratio of ergosterol: βsitosterol or in combination with the presence of brassicasterol can be used to distinguish this oil from plant oils.

[0061] In some embodiments, the oil content of an oil provided herein contains, as a percentage of total sterols, less than 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1% βsitosterol. In other embodiments the oil is free from β-sitosterol.

[0062] In some embodiments, the oil is free from one or more of β-sitosterol, campesterol, or stigmasterol. In some embodiments the oil is free from β-sitosterol, campesterol, and stigmasterol. In some embodiments the oil is free from campesterol.

In some embodiments the oil is free from stigmasterol.

[0063] In some embodiments, the oil content of an oil provided herein comprises, as a percentage of total sterols, less than 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1% 24ethylcholest-5-en-3-ol. In some embodiments, the 24-ethylcholest-5-en-3-ol is clionasterol. In some embodiments, the oil content of an oil provided herein comprises, as a percentage of total sterols, at least 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10% clionasterol.

[0064] In some embodiments, the oil content of an oil provided herein contains, as a percentage of total sterols, less than 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1% 24methylcholest-5-en-3-ol. In some embodiments, the 24-methylcholest-5-en-3-ol is

22, 23-dihydrobrassicasterol. In some embodiments, the oil content of an oil provided

2018267601 21 Nov 2018 herein comprises, as a percentage of total sterols, at least 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10% 22,23-dihydrobrassicasterol.

[0065] In some embodiments, the oil content of an oil provided herein contains, as a percentage of total sterols, less than 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1% 5,22cholestadien-24-ethyl-3-ol. In some embodiments, the 5, 22-cholestadien-24-ethyl-3ol is poriferasterol. In some embodiments, the oil content of an oil provided herein comprises, as a percentage of total sterols, at least 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10% poriferasterol.

[0066] In some embodiments, the oil content of an oil provided herein contains ergosterol or brassicasterol or a combination of the two. In some embodiments, the oil content contains, as a percentage of total sterols, at least 5%, 10%, 20%, 25%, 35%, 40%, 45%, 50%, 55%, 60%, or 65% ergosterol. In some embodiments, the oil content contains, as a percentage of total sterols, at least 25% ergosterol. In some embodiments, the oil content contains, as a percentage of total sterols, at least 40% ergosterol. In some embodiments, the oil content contains, as a percentage of total sterols, at least 5%, 10%, 20%, 25%, 35%, 40%, 45%, 50%, 55%, 60%, or 65% of a combination of ergosterol and brassicasterol.

[0067] In some embodiments, the oil content contains, as a percentage of total sterols, at least 1%, 2%, 3%, 4% or 5% brassicasterol. In some embodiments, the oil content contains, as a percentage of total sterols less than 10%, 9%, 8%, 7%, 6%, or 5% brassicasterol.

[0068] In some embodiments the ratio of ergosterol to brassicasterol is at least 5:1, 10:1, 15:1,or 20:1.

[0069] In some embodiments, the oil content contains, as a percentage of total sterols, at least 5%, 10%, 20%, 25%, 35%, 40%, 45%, 50%, 55%, 60%, or 65% ergosterol and less than 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1% β-sitosterol. In some embodiments, the oil content contains, as a percentage of total sterols, at least 25% ergosterol and less than 5% β-sitosterol. In some embodiments, the oil content further comprises brassicasterol.

[0070] Sterols contain from 27 to 29 carbon atoms (C27 to C29) and are found in all eukaryotes. Animals exclusively make C27 sterols as they lack the ability to further 29

2018267601 21 Nov 2018 modify the C27 sterols to produce C28 and C29 sterols. Plants however are able to synthesize C28 and C29 sterols, and C28/C29 plant sterols are often referred to as phytosterols. The sterol profile of a given plant is high in C29 sterols, and the primary sterols in plants are typically the C29 sterols b-sitosterol and stigmasterol. In 5 contrast, the sterol profile of non-plant organisms contain greater percentages of C27 and C28 sterols. For example the sterols in fungi and in many microalgae are principally C28 sterols. The sterol profile and particularly the striking predominance of C29 sterols over C28 sterols in plants has been exploited for determining the proportion of plant and marine matter in soil samples (Huang, Wen-Yen, Meinschein 10 W. G., “Sterols as ecological indicators”; Geochimica et Cosmochimia Acta. Vol 43.

pp 739-745).

[0071] In some embodiments the primary sterols in the microalgal oils provided herein are sterols other than b-sitosterol and stigmasterol. In some embodiments of the microalgal oils, C29 sterols make up less than 50%, 40%, 30%, 20%, 10%, or 5% 15 by weight of the total sterol content.

[0072] In some embodiments the microalgal oils provided herein contain C28 sterols in excess of C29 sterols. In some embodiments of the microalgal oils, C28 sterols make up greater than 50%, 60%, 70%, 80%, 90%, or 95% by weight of the total sterol content. In some embodiments the C28 sterol is ergosterol. In some embodiments the 20 C28 sterol is brassicasterol.

[0073] In embodiments of the present invention, oleaginous cells expressing one or more of the genes of Table 1 can produce an oil with at least 20, 40, 60 or 70% of C8, C10, C12, C14 or C16 fatty acids. In a specific embodiment, the level of myristate (C14:0) in the oil is greater than 30%.

[0074] Thus, in embodiments of the invention, there is a process for producing an oil, triglyceride, fatty acid, or derivative of any of these, comprising transforming a cell with any of the nucleic acids discussed herein. In another embodiment, the transformed cell is cultivated to produce an oil and, optionally, the oil is extracted.

Oil extracted in this way can be used to produce food, oleochemicals or other products.

2018267601 21 Nov 2018 [0075] The oils discussed above alone or in combination are useful in the production of foods, fuels and chemicals (including plastics, foams, films, etc). The oils, triglycerides, fatty acids from the oils may be subjected to C-H activation, hydroamino methylation, methoxy-carbonation, ozonolysis, enzymatic transformations, epoxidation, methylation, dimerization, thiolation, metathesis, hydroalkylation, lactonization, or other chemical processes.

[0076] After extracting the oil, a residual biomass may be left, which may have use as a fuel, as an animal feed, or as an ingredient in paper, plastic, or other product. For example, residual biomass from heterotrophic algae can be used in such 10 products.

Example 1. Discovery of Novel FATB sequences [0077] Sequences of novel plant acyl-ACP thioesterases involved in seed-specific mid-chain (C8-C16) fatty acid biosynthesis in higher plants were isolated. Seed-specific lipid production genes were isolated through direct interrogation of RNA pools accumulating in oilseeds. Based on phylogenetic analysis, novel enzymes can be classified as members of FatB family of acyl-ACP thioesterases.

[0078] Seeds of oleaginous plants were obtained from local grocery stores or requested through USDA ARS National Plant Germplasm System (NPGS) from 20 North Central Regional Plant Introduction Station (NCRIS) or USDA ARS North

Central Soil Conservation Research Laboratory (Morris, MI). Dry seeds were homogenized in liquid nitrogen to powder, resuspended in cold extraction buffer containing 6-8M Urea and 3M LiCl and left on ice for a few hours to overnight at 4 °C. The seed homogenate was passed through NucleoSpin Filters (Macherey-Nagel) 25 by centrifugation at 20,000g for 20 minutes in the refrigerated micro centrifuge (4 °C).

The resulting RNA pellets were resuspended in the buffer containing 20 mM Tris HC1, pH7.5, 0.5% SDS, 100 mM NaCl, 25 mM EDTA, 2% PVPP) and RNA was subsequently extracted once with Phenol-Chloroform-Isoamyl Alcohol (25:24:1, v/v) and once with chloroform. RNA was finally precipitated with isopropyl alcohol (0.7 30 Vol.) in the presence of 150 mM of Na Acetate, pH5.2, washed with 80% ethanol by centrifugation, and dried. RNA samples were treated with Turbo DNAse (Lifetech) and purified further using RNeasy kits (Qiagen) following manufacturers’ protocols.

2018267601 21 Nov 2018

The resulting purified RNA samples were converted to pair-end cDNA libraries and subjected to next-generation sequencing (2xl00bp) using Illumina Hiseq 2000 platform. RNA sequence reads were assembled into corresponding seed transcriptomes using Trinity or Oases packages. Putative thioesterase-containg cDNA contigs were identified by mining transcriptomes for sequences with homology to known thioesterases. These in silico identified putative thioesterase cDNAs have been further verified by direct reverse transcription PCR analysis using seed RNA and primer pairs targeting full-length thioesterase cDNAs. The resulting amplified products were cloned and sequenced de novo to confirm authenticity of identified thioesterase genes.

[0079] To interrogate evolutionary and functional relationship between novel acyl-ACP thioesterases and the members of two existing thioesterase classes (FatA and FatB), we performed a phylogenetic analysis using published full-length (Mayer and Shanklin, 2007) and truncated (THYME database) amino acid thioesterase sequences. Novel proteins appear to group with known acyl-ACP FatB thioesterases involved in biosynthesis of C8-C16 fatty acids. Moreover, novel thioesterases appear to cluster into 3 predominant out-groups suggesting distinct functional similarity and evolutionary relatedness among members of each cluster.

[0080] The amino acid sequences of the FatB genes follow are shown in Table

4.

Table 4: Amino acid sequences of FatB genes:

CuPSR23 FATB3	SEQ ID NO: 110
CuPSR23 FATB3b	SEQ ID NO: 111
CwFATB3	SEQ ID NO: 112
CwFATB3a	SEQ ID NO: 113
CwFATB3b	SEQ ID NO: 114
CwFATB3c	SEQ ID NO: 115
CwFATB4a	SEQ ID NO: 116
CwFATB4a.l	SEQ ID NO: 117
CwFATB4a.2	SEQ ID NO: 118
CwFATB4a.3	SEQ ID NO: 119

2018267601 21 Nov 2018

CwFATB4b	SEQ ID NO: 120
CwFATB4b.l	SEQ ID NO: 121
CwFATBS	SEQ ID NO: 122
CwFATBSa	SEQ ID NO: 123
CwFATBSb	SEQ ID NO: 124
CwFATBSc	SEQ ID NO: 125
CwFATBS. 1	SEQ ID NO: 126
CwFATBS.la	SEQ ID NO: 127
CcFATB2b	SEQ ID NO: 128
CcFATB3	SEQ ID NO: 129
CcFATB3b	SEQ ID NO: 130
CcFATB3c	SEQ ID NO: 131
ChtFATBla	SEQ ID NO: 132
ChtFATBla.l	SEQ ID NO: 133
ChtFATBla.2	SEQ ID NO: 134
ChtFATBla.3	SEQ ID NO: 135
ChtFATBla.4	SEQ ID NO: 136
ChtFATBlb	SEQ ID NO: 137
ChtFATB2b	SEQ ID NO: 138
ChtFATB2a	SEQ ID NO: 139
ChtFATB2c	SEQ ID NO: 140
ChtFATB2d	SEQ ID NO: 141
ChtFATB2e	SEQ ID NO: 142
ChtFATB2f	SEQ ID NO: 143
ChtFATB2g	SEQ ID NO: 144
ChtFATB2h	SEQ ID NO: 145
ChtFATB3a	SEQ ID NO: 146
ChtFATB3b	SEQ ID NO: 147
ChtFATB3c	SEQ ID NO: 148
ChtFATB3d	SEQ ID NO: 149
ChtFATB3e	SEQ ID NO: 150
ChtFATB3f	SEQ ID NO: 151

2018267601 21 Nov 2018

ChtFATB3g	SEQ ID NO: 152
ChsFATBl	SEQ ID NO: 153
ChsFATB2	SEQ ID NO: 154
ChsFatB2b	SEQ ID NO: 155
ChsFatB2c	SEQ ID NO: 156
ChsFatB2d	SEQ ID NO: 157
Chs FATB3	SEQ ID NO: 158
ChsFatb3b	SEQ ID NO: 159
ChsFatB3c	SEQ ID NO: 160
ChsFATB3d	SEQ ID NO: 161
ChsFATB3e	SEQ ID NO: 162
ChsFATB3f	SEQ ID NO: 163
ChsFATB3g	SEQ ID NO: 164
ChsFATB3h	SEQ ID NO: 165
ChsFATB3i	SEQ ID NO: 166
ChsFATB3j	SEQ ID NO: 167

ChsFATB3j:

MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQV

KANASARPKANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITT

VFVAAEKQWTMLDRKSKRPDMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGA

DRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWVVTKMHIEV

NRYPTWGDTIEVNTWVSESGKTGMGRDWLISDFHTGDILIRATSVCAMMNQ

KTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRW

NDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRQECGRDSVLESV

TAMDPSKEGDRSLYQHLLRLEDGTDIAKGRTKWRPKNAGKTSNGNSIS

2018267601 21 Nov 2018

Example 2. Cloning and fatty acid analysis of cells transformed with novel FATB genes [0081] In the example below, we detail the effect of expressing plant oilseed transcriptome-derived, heterologous thioesterases in the UTEX1435 (web.biosci.utexas.edu/utex/) strain, Strain A.

[0082] As in Example 1, RNA was extracted from dried plant seeds and submitted for paired-end sequencing using the Illumina Hiseq 2000 platform. RNA sequence reads were assembled into corresponding seed transcriptomes using Trinity or Oases packages and putative thioesterase-containing cDNA contigs were identified 10 by mining transcriptomes for sequences with homology to known thioesterases.

These in silico identified putative thioesterase cDNAs were verified by direct reverse transcription PCR analysis using seed RNA and primer pairs targeting full-length thioesterase cDNAs. The resulting amplified products were cloned and sequenced de novo to confirm authenticity of identified thioesterase genes and to identify sequence 15 variants arising from expression of different gene alleles or diversity of sequences within a population of seeds. The resulting amino acid sequences were subjected to phylogenetic analysis using published full-length (Mayer and Shanklin, 2007) and truncated (THYME database) FatB sequences. The thioesterases that clustered with acyl-ACP FatB thioesterases, which are involved in biosynthesis of C8-C16 fatty 20 acids, were pursued.

Construction of Transforming Vectors Expressing Acyl-ACP FatB Thioesterases [0083] 27 putative acyl-ACP FatB thioesterases from the species

Cinnamomum camphora, Cuphea hyssopifolia, Cuphea PSR23, Cuphea wrightii, Cuphea heterophylla, and Cuphea viscosissima were synthesized in a codon25 optimized form to reflect Prototheca moriformis (UTEX 1435) codon usage. Of the 27 genes synthesized, 24 were identified by our transcriptome sequencing efforts and the 3 genes from Cuphea viscosissima, were from published sequences in GenBank.

[0084] Transgenic strains were generated via transformation of the base strain

Strain A (Prototheca moriformis, derived from UTEX 1435 by classical mutation and 30 screening for high oil production) with a construct encoding 1 of the 27 FatB thioesterases. The construct pSZ2760 encoding Cinnamomum camphora (Cc)

FATBlb is shown as an example, but identical methods were used to generate each of

2018267601 21 Nov 2018 the remaining 26 constructs encoding the different respective thioesterases. Construct pSZ2760 can be written as 6S::CrTUB2:ScSUC2:CvNR::PmAMT3:CcFATBlb:CvNR::6S. The sequence of the transforming DNA is provided in Table 5 (pSZ2760). The relevant restriction sites in the construct from 5’-3’, BspQl, Kpnl, Asci, Mfel, EcoRI, Spel, Xhol, SacI, BspQl, respectively, are indicated in lowercase, bold, and underlined. BspQl sites delimit the 5 ’ and 3 ’ ends of the transforming DNA. Bold, lowercase sequences at the 5 ’ and 3 ’ end of the construct represent genomic DNA from UTEX 1435 that target integration to the 6S locus via homologous recombination. Proceeding in the 5 ’ to 3 ’ direction, the selection cassette has the C. reinhardtii β-tubulin promoter driving expression of the S. cerevisiae gene SUC2 (conferring the ability to grow on sucrose) and the Chlorella vulgaris Nitrate Reductase (NR) gene 3 ’ UTR. The promoter is indicated by lowercase, boxed text. The initiator ATG and terminator TGA for ScSUC2 are indicated by bold, uppercase italics, while the coding region is indicated with lowercase italics. The 3 ’ UTR is indicated by lowercase underlined text. The spacer region between the two cassettes is indicated by upper case text. The second cassette containing the codon optimized CcFATBlb gene (Table 5; pSZ2760) from Cinnamomum camphora is driven by the Prototheca moriformis endogenous AMT3 promoter, and has the Chlorella vulgaris Nitrate Reductase (NR) gene 3 ’ UTR. In this cassette, the AMT3 promoter is indicated by lowercase, boxed text. The initiator ATG and terminator TGA for the CcFATBlb gene are indicated in bold, uppercase italics, while the coding region is indicated by lowercase italics and the spacer region is indicated by upper case text. The 3 ’ UTR is indicated by lowercase underlined text. The final construct was sequenced to ensure correct reading frame and targeting sequences.

Table 5: pSZ2760 Transforming construct gctcttcgccgccgccactcctgctcgagcgcgcccgcgcgtgcgccgccagcgccttggccttttcgccgcgctcgtgc gcgtcgctgatgtccatcaccaggtccatgaggtctgccttgcgccggctgagccactgcttcgtccgggcggccaagag gagcatgagggaggactcctggtccagggtcctgacgtggtcgcggctctgggagcgggccagcatcatctggctctgc cgcaccgaggccgcctccaactggtcctccagcagccgcagtcgccgccgaccctggcagaggaagacaggtgaggg gggtatgaattgtacagaacaaccacgagccttgtctaggcagaatccctaccagtcatggctttacctggatgacggcctg

2018267601 21 Nov 2018 cgaacagctgtccagcgaccctcgctgccgccgcttctcccgcacgcttctttccagcaccgtgatggcgcgagccagcg ccgcacgctggcgctgcgcttcgccgatctgaggacagtcggggaactctgatcagtctaaacccccttgcgcgttagtgtt gccatcctttgcagaccggtgagagccgacttgttgtgcgccaccccccacaccacctcctcccagaccaattctgtcacct ttttggcgaaggcatcggcctcggcctgcagagaggacagcagtgcccagccgctgggggttggcggatgcacgctca ggtacc|ctttcttgcgctatgacacttccagcaaaaggtagggcgggctgcgagacggcttcccggcgctgcatgcaaca ccgatgatgcttcgaccccccgaagctccttcggggctgcatgggcgctccgatgccgctccagggcgagcgctgtttaa atagccaggcccccgattgcaaagacattatagcgagctaccaaagccatattcaaacacctagatcactaccacttctaca ^cATGctgctgcaggccttcctgttcctgctggccggcttcgccgccaagatcagcgcctccatgacgaacgagac gtccgaccgccccctggtgcacttcacccccaacaagggctggatgaacgaccccaacggcctgtggtacgacgag aaggacgccaagtggcacctgtacttccagtacaacccgaacgacaccgtctgggggacgcccttgttctggggcca cgccacgtccgacgacctgaccaactgggaggaccagcccatcgccatcgccccgaagcgcaacgactccggcgc cttctccggctccatggtggtggactacaacaacacctccggcttcttcaacgacaccatcgacccgcgccagcgctgc gtggccatctggacctacaacaccccggagtccgaggagcagtacatctcctacagcctggacggcggctacaccttc accgagtaccagaagaaccccgtgctggccgccaactccacccagttccgcgacccgaaggtcttctggtacgagcc ctcccagaagtggatcatgaccgcggccaagtcccaggactacaagatcgagatctactcctccgacgacctgaagt cctggaagctggagtccgcgttcgccaacgagggcttcctcggctaccagtacgagtgccccggcctgatcgaggtcc ccaccgagcaggaccccagcaagtcctactgggtgatgttcatctccatcaaccccggcgccccggccggcggctcct tcaaccagtacttcgtcggcagcttcaacggcacccacttcgaggccttcgacaaccagtcccgcgtggtggacttcgg caaggactactacgccctgcagaccttcttcaacaccgacccgacctacgggagcgccctgggcatcgcgtgggcctc caactgggagtactccgccttcgtgcccaccaacccctggcgctcctccatgtccctcgtgcgcaagttctccctcaaca ccgagtaccaggccaacccggagacggagctgatcaacctgaaggccgagccgatcctgaacatcagcaacgccg gcccctggagccggttcgccaccaacaccacgttgacgaaggccaacagctacaacgtcgacctgtccaacagcac cggcaccctggagttcgagctggtgtacgccgtcaacaccacccagacgatctccaagtccgtgttcgcggacctctcc ctctggttcaagggcctggaggaccccgaggagtacctccgcatgggcttcgaggtgtccgcgtcctccttcttcctgga ccgcgggaacagcaaggtgaagttcgtgaaggagaacccctacttcaccaaccgcatgagcgtgaacaaccagcc cttcaagagcgagaacgacctgtcctactacaaggtgtacggcttgctggaccagaacatcctggagctgtacttcaac gacggcgacgtcgtgtccaccaacacctacttcatgaccaccgggaacgccctgggctccgtgaacatgacgacggg ggtggacaacctgttctacatcgacaagttccaggtgcgcgaggtcaagTGAcaattggcagcagcagctcggatag tatcgacacactctggacgctggtcgtgtgatggactgttgccgccacacttgctgccttgacctgtgaatatccctgccgctt ttatcaaacagcctcagtgtgtttgatcttgtgtgtacgcgcttttgcgagttgctagctgcttgtgctatttgcgaataccacccc cagcatccccttccctcgtttcatatcgcttgcatcccaaccgcaacttatctacgctgtcctgctatccctcagcgctgctcct gctcctgctcactgcccctcgcacagccttggtttgggctccgcctgtattctcctggtactgcaacctgtaaaccagcactg

2018267601 21 Nov 2018

ggcaccaagttctcctacaccgagtccctgaagaagctgcccgactggtccatgctgttcgccgtgatcaccaccatctt ctccgccgccgagaagcagtggaccaacctggagtggaagcccaagcccaaccccccccagctgctggacgacca cttcggcccccacggcctggtgttccgccgcaccttcgccatccgctcctacgaggtgggccccgaccgctccacctcc 20 atcgtggccgtgatgaaccacctgcaggaggccgccctgaaccacgccaagtccgtgggcatcctgggcgacggctt cggcaccaccctggagatgtccaagcgcgacctgatctgggtggtgaagcgcacccacgtggccgtggagcgctacc ccgcctggggcgacaccgtggaggtggagtgctgggtgggcgcctccggcaacaacggccgccgccacgacttcct ggtgcgcgactgcaagaccggcgagatcctgacccgctgcacctccctgtccgtgatgatgaacacccgcacccgcc gcctgtccaagatccccgaggaggtgcgcggcgagatcggccccgccttcatcgacaacgtggccgtgaaggacga 25 ggagatcaagaagccccagaagctgaacgactccaccgccgactacatccagggcggcctgaccccccgctggaa cgacctggacatcaaccagcacgtgaacaacatcaagtacgtggactggatcctggagaccgtgcccgactccatctt cgagtcccaccacatctcctccttcaccatcgagtaccgccgcgagtgcacccgcgactccgtgctgcagtccctgacc accgtgtccggcggctcctccgaggccggcctggtgtgcgagcacctgctgcagctggagggcggctccgaggtgct gcgcgccaagaccgagtggcgccccaagctgtccttccgcggcatctccgtgatccccgccgagtcctccgtgatgga 30 ctacaaggaccacgacggcgactacaaggaccacgacatcgactacaaggacgacgacgacaagTGActcga ggcagcagcagctcggatagtatcgacacactctggacgctggtcgtgtgatggactgttgccgccacacttgctgccttg acctgtgaatatccctgccgcttttatcaaacagcctcagtgtgtttgatcttgtgtgtacgcgcttttgcgagttgctagctgctt

2018267601 21 Nov 2018 gtgctatttgcgaataccacccccagcatccccttccctcgtttcatatcgcttgcatcccaaccgcaacttatctacgctgtcc tgctatccctcagcgctgctcctgctcctgctcactgcccctcgcacagccttggtttgggctccgcctgtattctcctggtact gcaacctgtaaaccagcactgcaatgctgatgcacgggaagtagtgggatgggaacacaaatggaAAGCTGTAT AGGGATAACAGGGTAATgagctcttgttttccagaaggagttgctccttgagcctttcattctcagcctcgata 5 acctccaaagccgctctaattgtggagggggttcgaatttaaaagcttggaatgttggttcgtgcgtctggaacaagcccag acttgttgctcactgggaaaaggaccatcagctccaaaaaacttgccgctcaaaccgcgtacctctgctttcgcgcaatctgc cctgttgaaatcgccaccacattcatattgtgacgcttgagcagtctgtaattgcctcagaatgtggaatcatctgccccctgtg cgagcccatgccaggcatgtcgcgggcgaggacacccgccactcgtacagcagaccattatgctacctcacaatagttca taacagtgaccatatttctcgaagctccccaacgagcacctccatgctctgagtggccaccccccggccctggtgcttgcg 10 gagggcaggtcaaccggcatggggctaccgaaatccccgaccggatcccaccacccccgcgatgggaagaatctctcc ccgggatgtgggcccaccaccagcacaacctgctggcccaggcgagcgtcaaaccataccacacaaatatccttggcat cggccctgaattccttctgccgctctgctacccggtgcttctgtccgaagcaggggttgctagggatcgctccgagtccgca aacccttgtcgcgtggcggggcttgttcgagcttgaagagc [0085] Constructs encoding the identified heterologous FatB genes, such as

CcFATBlb from pSZ2760 in Table 6, were transformed into Strain A, and selected for the ability to grow on sucrose. Transformations, cell culture, lipid production and fatty acid analysis were all carried out as previously described. After cultivating on sucrose under low nitrogen conditions to accumulate oil, fatty acid profiles were determined by FAME-GC. The top performer from each transformation, as judged by the ability to produce the highest level of midchain fatty acids, is shown in Table 4.

2018267601 21 Nov 2018

Table 6: Alteration of Fatty Acid Profiles in S3150 upon Expression of Heterologous

FatB Thioesterases

		FA profile of top performer from each	transformation (%; primary lipid in	Strain A	background)
Species	Gene Name	SZ Plasmid	Strain	C8:0	| C10:0	C12:0	C14:0	C16:0	C18:0	C18:l	C18:2	C18:3a
Cinnamomum camphora	CcFATBlb	pSZ2760		0	0	1	15	26	2	46	9	1
Cinnamomum camphorct	CcFATB4	pSZ2756	/OWStOBSiSiSii	0	1	33	4	7	2	41	10	1
Cinnamomum camphora	CcFATB3	pSZ2755		0	0	0	3	44	3	41	8	0
Cuphea hyssopifolia	ChsFATBl	pSZ2778		0	0	0	2	22	4	63	8	1
Cuphea hyssopifolia	ChsFATB2	pSZ2796		0	0	0	6	53	3	32	6	0
Cuphea hyssopifolia	ChsFATB2b	pSZ2792		0	0	0	5	26	2	56	9	1
Cuphea hyssopifolia	ChsFATB3	pSZ2797		0	0	8	34	27	2	24	5	1
Cuphea hyssopifolia	ChsFATB3b	pSZ2795		0	0	7	29	27	1	28	6	1
Cuphea PSR23	CuPSR23FATB3	pSZ2793		0	1	0	2	24	3	61	8	1
Cuphea wrightii	CwFATB3	pSZ2751		0	2	17	9	19	2	41	8	1
Cuphea wrightii	CwFATB4a	pSZ2752		0	0	0	4	48	3	36	7	1
Cuphea wrightii	CwFATB4b	pSZ2753		0	0	0	5	52	3	32	6	1
Cuphea wrightii	CwFATB5	pSZ2754		0	0	0	3	27	3	57	7	1
Cuphea heterophylla	ChtFATBla	pSZ2757		0	0	5	18	27	2	39	7	1
Cuphea heterophylla	ChtFATBlb	pSZ2773		0	0	2	7	27	3	53	8	1
Cuphea heterophylla	ChtFATB2b	pSZ2780		0	0	0	2	25	3	61	8	1
Cuphea heterophylla	ChtFATB2a	pSZ2774		0	i 0	0	2	27	3	59	6	0
Cuphea heterophylla	ChtFATB2c	pSZ2758		0	0	3	2	23	3	58	7	1
Cuphea heterophylla	ChtFATB2d	pSZ2759		0	0	4	4	23	3	54	9	1
Cuphea heterophylla	ChtFATB2e	pSZ2775		0	1	2	3	24	3	57	8	1
Cuphea heterophylla	ChtFATB2f	pSZ2777		0	0	0	2	28	3	57	8	1
Cuphea heterophylla	ChtFATB2g	pSZ2794		0	0	0	2	22	3	62	9	1
Cuphea heterophylla	ChtFATB3a	pSZ2776		0	0	0	5	47	4	37	7	1
Cuphea heterophylla	ChtFATB3b	pSZ2779		0	0	0	6	49	5	32	7	0
Cuphea viscosissima	CvisFATBl	pSZ2810		0	1	0	2	24	3	60	8	0
Cuphea viscosissima	CvisFATB2	pSZ2817		0	: 0	0	4	51	2	36	6	0
Cuphea viscosissima	CvisFATB3	pSZ2791		0	0	0	8	28	2	52	8	1
		A (parent strain):	llOil		0	2		1^311	58		0

[0086] Many of the acyl-ACP FatB thioesterases were found to exhibit midchain activity when expressed in Prototheca moriformis. For example, expression of CcFATBlb causes an increase in myristate levels from 2% of total fatty acids in the parent, Strain A, to ~ 15% in the DI670-13 primary transformant. Other examples include CcFATB4, which exhibits an increase in laurate levels from 0% in Strain A to ~33%, and ChsFATB3, which exhibits an increase in myristate levels to ~34%.

Although some of the acyl-ACP thioesterases did not exhibit dramatic effects on midchain levels in the current incarnation, efforts will likely develop to optimize some of these constructs.

[0087] Sequences of the Heterologous Acyl-ACP Thioesterases Identified and

Transformed into P. moriformis (UTEX 1435) [0088] A complete listing of relevant sequences for the transforming constructs, such as the deduced amino acid sequence of the encoded acyl-ACP thioesterase, the native CDS coding sequence, the Prototheca moriformis codon40

2018267601 21 Nov 2018 optimized coding sequence, and the nature of the sequence variants examined, is provided as SEQ ID NOS: 1-78.

Example 3. Discovery and Cloning of Additional FATB genes

Additional FATB genes were obtained from seeds as described above. The species 5 and number of FatB genes identified were:

Species	Accession Number	Novel FatB Thioesterase Genes
Cuphea calcarata	534665	1
Cuphea painteri	288248	1
Cuphea hookeriana	534896	1
Cuphea avigera var. pulcherrima	Ames 17868	1
Cuphea paucipetala	534877	1
Cuphea procumbens	534881	3
Cuphea ignea	534773	1

[0089] The thioesterases that clustered with acyl-ACP FatB thioesterases, which are involved in biosynthesis of C8-C16 fatty acids, were pursued. The native, 10 putative plastid-targeting transit peptide sequence is indicated by underlining.

[0090] Construction of Transforming Vectors Expressing Acyl-ACP FatB

Thioesterases. The nine putative Acyl-ACP FatB Thioesterases from the species Cuphea calcarata, Cuphea painter, Cuphea hookeriana, Cuphea avigera var. pulcherrima, Cuphea paucipetala, Cuphea procumbens, and Cuphea ignea were synthesized in a codon-optimized form to reflect UTEX 1435 codon usage. In contrast to the previous example, the new Acyl-ACP FatB thioesterases were synthesized with a modified transit peptide from Chlorella protothecoides (Cp) in place of the native transit peptide. The modified transit peptide derived from the CpSADl gene, “CpSADltp_trimmed”, was synthesized as an in-frame, N-terminal

2018267601 21 Nov 2018 fusion to the FatB acyl-ACP thioesterases in place of the native transit peptide; the resulting sequences are listed below. The novel FatB genes were cloned into Prototheca moriformis as described above. Constructs encoding heterologous FatB genes were transformed into strain S6165 (a descendant of S3150/Strain A) and selected for the ability to grow on sucrose. Transformations, cell culture, lipid production and fatty acid analysis were all carried out as previously described. The results for the nine novel FatB acyl-ACP thioesterases are displayed in the table immediately below.

2018267601 21 Nov 2018

[0091] Of particular note are: CpaiFATBl, which exhibits 17% C10:0 and 8%

C8:0 fatty acid levels; CpauFATBl, which exhibits 9% C10:0 and 1% C12:0 fatty

2018267601 21 Nov 2018 acid levels; CigneaFATBl, which exhibits 8% C10:0 and 1% C12:0 fatty acid levels; CcalcFATBl, which exhibits 18% C14:0 and 12% C12:0 levels; and CaFATBl, which exhibits 22% C8:0 and 9% C10:0 fatty acid levels.

[0092] CaFATBl, which exhibits high C8:0 and C10:0 levels, is of particular interest. CaFATBl arose from two separate contigs that were assembled from the Cupha avigera var. pulcherrima transcriptome, S17_Cavig_trinity_7406 and S17_Cavig_trinity_7407. Although the two partial contigs exhibit only 17 nucleotides of overlap, we were able to assemble a putative full length transcript encoding CaFATBl from the two contigs and then subsequently confirm the existence of the full-length transcript by direct reverse transcription PCR analysis using seed RNA and primer pairs targeting the full-length CaFATBl thioesterase cDNA. Tjellstrom et al. (2013) discloses the expression of a newly identified fatty acyl-ACP thioesterase from Cuphea pulcherrima that they named “CpuFATB3” (Genbank accession number KC675178). The coding sequence of CpuFATB3 is

100% identical to the CaFATBl gene we identified and contains one nucleotide difference in the RNA sequence outside the predicted coding region. Tjellstrom et al. (2013) showed that CpuFATB3 produces an average of 4.8% C8:0 when expressed in Arabidopsis, and further requires deletion of two acyl-ACP synthetases, AAE15/16, to produce an average of 9.2% C8:0 with a maximum level of ~12% C8.0. The

CaFATBl gene we identified was codon-optimized for expression in UTEX1435 and generated as a CpSADltp-trimmed transit peptide fusion before introduction into S6165. The CpSADltp_trimmed:CaFATBl gene produces an average C8:0 level of 14% and a maximum level of 22% C8:0 without requiring the deletion of endogenous acyl-ACP synthetases.

[0093] Table 7. Amino Acid Sequences of Additional Novel FatB Acyl-ACP

Thioesterases. In the appended sequence listings, the native, putative plastid-targeting transit peptide sequence is underlined:

FatB	Sequence ID NO:
CcalcFATBl (Cuphea calcar ata FATB1)	SEQ ID NO: 168
ChookFATB4 (Cuphea hookeriana FATB4)	SEQ ID NO: 169
CaFATBl (Cuphea avigera var. pulcherrima FATB1)	SEQ ID NO: 170

2018267601 21 Nov 2018

CpauFATBl (Cuphea paucipetala FATB1)	SEQ ID NO: 171
CprocFATBl (Cuphea procumbens FATB1)	SEQ ID NO: 172
CprocFATB2 (Cuphea procumbens FATB2)	SEO ID NO: 173
CprocFATB3 (Cuphea procumbens FATB3)	SEO ID NO: 174
CigneaFATBl (Cuphea ignea FATB 1)	SEO ID NO: 175
CcalcFATBl (Cuphea calcar ata FATB1)	SEO ID NO: 176
ChookFATB4 (Cuphea hookeriana FATB4)	SEO ID NO: 177
CaFATBl (Cuphea avigera var. pulcherrima FATB1)	SEO ID NO: 178
CpauFATBl (Cupheapaucipetala FATB1)	SEO ID NO: 179
CprocFATBl (Cuphea procumbens FATB1)	SEO ID NO: 180
CprocFATB2 (Cuphea procumbens FATB2)	SEO ID NO: 181
CprocFATB3 (Cuphea procumbens FATB3)	SEO ID NO: 182
CigneaFATBl (Cuphea ignea FATB7)	SEO ID NO: 183

Example 4. FATB consensus sequences: Discovery, cloning and fatty acid profiles [0094] In the course of testing several new putative midchain FatB thioesterases in UTEX1435, S3150 (Strain A above), we identified several thioesterases with increased Cl0:0 and Cl6:0 activity above the background midchain levels found in the strain. We reasoned that a consensus sequence could be obtained for an idealized C10:0 thioesterase and C16:0 thioesterase from aligning the bestperforming C10:0 and C16:0 thioesterases. A consensus C10:0 specific thioesterase sequence was generated using the C. palustris FatBl (CpFATBl), C. PSR23 FatB3 (CuPSR23FATB3), C. viscosissima FatBl (CvisFATBl), C. glossostoma FatBl (CgFATBl), and C. carthagenensis FatB2 (CcrFATB2) sequences as inputs resulting in a C10:0 specific consensus sequence termed JcFATBl/SzFATBl. A consensus Cl6:0 specific thioesterase sequence was generated using the C. heterophylla FatB3a (ChtFATB3a), C. carthagenensis FatBl (CcrFATBl), C. viscosissima FatB2 (CvisFATB2), C. hookeriana FatBl (ChFATBl; AAC48990), C. hyssopifolia FatB2 (ChsFATB2), C. calophylla FatB2 (CcalFATB2; ABB71581), C. hookeriana FatBl-1 (ChFATBl-1; AAC72882), C. lanceolata FatBl (C1FATB1; CAC19933), and C. wrightii FatB4a (CwFATB4a) sequences as inputs resulting in a Cl6:0 specific consensus sequence termed JcFATB2/SzFATB2. The resulting consensus sequences

2018267601 21 Nov 2018 were synthesized, cloned into a vector identical to that used to test other FatB thioesterases, and introduced into S3150 as described above. The consensus amino acid sequences are given as SEQ ID NOs. 106 and 107; the nucleic acid sequences were based on these amino acid sequences using codon optimization for Prototheca moriformis. The transformants were selected, cultivated and the oil was extracted and analyzed by FAME-GC-FID. The fatty acid profiles obtained are given in the table below.

FA profile of top performer from each transformation (%; primary lipid)

Gene

SZ

C

C

C

C

C

C

C

C

Cl

Nam

Plas

8:

10

12

14

16

18

18

18

8:3

Species

e

mid

Strain

0

:0

:0

:0

:0

:0

:1

:2

a

Consensu

s

S3150;

Sequence

JcFA

pSZ3

T617;

1 Consensu

TB1

187

DI 930-18

0

2

0

2

26

3

57

8

1

s

S3150;

Sequence

JcFA

pSZ3

T600;

2

TB2

100

DI 872-17

0

0

0

6

54

3

29

6

0

S3150 (parent strain):

III

III

III

111

Illi

3

Illi

7

Illi

Examples: Clade analysis

Various novel FATB thioesterases were clustered according to a neighbor joining algorithm. These were found to form twelve clades as listed in Table la. Putative function was assigned based on expression in Prototheca as described above.

[0095] The described embodiments of the invention are intended to be merely exemplary and numerous variations and modifications will be apparent to those skilled in the art. All such variations and modifications are intended to be within the scope of the present invention.

2018267601 21 Nov 2018

Sequence Listing

SEQ ID NO 1:

Cinnamomum camphora (Cc) FATBlb variant M25L, M322R, AT367-D368 amino acid sequence

MATTSLASAFCSMKAVMLARDGRGLKPRSSDLQLRAGNAQTSLKMINGTKFSYTESLKKLPD WSMLFAVITTIFSAAEKQWTNLEWKPKPNPPQLLDDHFGPHGLVFRRTFAIRSYEVGPDRSTSI VAVMNHLQEAALNHAKSVGILGDGFGTTLEMSKRDLIWVVKRTHVAVERYPAWGDTVEVE CWVGASGNNGRRHDFLVRDCKTGEILTRCTSLSVMMNTRTRRLSKIPEEVRGEIGPAFIDNVA VKDEEIKKPQKLNDSTADYIQGGLTPRWNDLDINQHVNNIKYVDWILETVPDSIFESHHISSFTI

EYRRECTRDSVLQSLTTVSGGSSEAGLVCEHLLQLEGGSEVLRAKTEWRPKLSFRGISVIPAES SV*

SEQ ID NO 2:

Cinnamomum camphora (Cc) FATBlb variant M25L, M322R, AT367-D368 coding DNA sequence

TTAGCTTCTGCTTTCTGCTCGATGAAAGCTGTAATGTTGGCTCGTGATGGCAGGGGCTTGA

AACCCAGGAGCAGTGATTTGCAGCTGAGGGCGGGAAATGCACAAACCTCTTTGAAGATGA

TCAATGGGACCAAGTTCAGTTACACAGAGAGCTTGAAAAAGTTGCCTGACTGGAGCATGC TCTTTGCAGTGATCACGACCATCTTTTCGGCTGCTGAGAAGCAGTGGACCAATCTAGAGTG

GAAGCCGAAGCCGAATCCACCCCAGTTGCTTGATGACCATTTTGGGCCGCATGGGTTAGTT

TTCAGGCGCACCTTTGCCATCAGATCGTATGAGGTGGGACCTGACCGCTCCACATCTATAG

TGGCTGTTATGAATCACTTGCAGGAGGCTGCACTTAATCATGCGAAGAGTGTGGGAATTCT

AGGAGATGGATTCGGTACGACGCTAGAGATGAGTAAGAGAGATCTGATATGGGTTGTGAA

ACGCACGCATGTTGCTGTGGAACGGTACCCTGCTTGGGGTGATACTGTTGAAGTAGAGTG

CTGGGTTGGTGCATCGGGAAATAATGGCAGGCGCCATGATTTCCTTGTCCGGGACTGCAA

AACAGGCGAAATTCTTACAAGATGTACCAGTCTTTCGGTGATGATGAATACAAGGACAAG

GAGGTTGTCCAAAATCCCTGAAGAAGTTAGAGGGGAGATAGGGCCTGCATTCATTGATAA

TGTGGCTGTCAAGGACGAGGAAATTAAGAAACCACAGAAGCTCAATGACAGCACTGCAG

ATTACATCCAAGGAGGATTGACTCCTCGATGGAATGATTTGGATATCAATCAGCACGTTA ACAACATCAAATACGTTGACTGGATTCTTGAGACTGTCCCAGACTCAATCTTTGAGAGTCA

TCATATTTCCAGCTTCACTATTGAATACAGGAGAGAGTGCACGAGGGATAGCGTGCTGCA

GTCCCTGACCACTGTCTCCGGTGGCTCGTCGGAAGCTGGGTTAGTGTGCGAGCACTTGCTC CAGCTTGAAGGTGGGTCTGAGGTATTGAGGGCAAAAACAGAGTGGAGGCCTAAGCTTAGT TTCAGAGGGATTAGTGTGATACCCGCAGAATCGAGTGTCTAA

SEQ ID NO 3:

Cinnamomum camphora (Cc) FATBlb variant M25L, M322R, AT367-D368 coding DNA sequence codon optimized for Prototheca moriformis

TTAGCTTCTGCTTTCTGCTCGATGAAAGCTGTAATGTTGGCTCGTGATGGCAGGGGCTTGA

AACCCAGGAGCAGTGATTTGCAGCTGAGGGCGGGAAATGCACAAACCTCTTTGAAGATGA

TCAATGGGACCAAGTTCAGTTACACAGAGAGCTTGAAAAAGTTGCCTGACTGGAGCATGC 40 TCTTTGCAGTGATCACGACCATCTTTTCGGCTGCTGAGAAGCAGTGGACCAATCTAGAGTG

GAAGCCGAAGCCGAATCCACCCCAGTTGCTTGATGACCATTTTGGGCCGCATGGGTTAGTT

TTCAGGCGCACCTTTGCCATCAGATCGTATGAGGTGGGACCTGACCGCTCCACATCTATAG

TGGCTGTTATGAATCACTTGCAGGAGGCTGCACTTAATCATGCGAAGAGTGTGGGAATTCT

AGGAGATGGATTCGGTACGACGCTAGAGATGAGTAAGAGAGATCTGATATGGGTTGTGAA 45 ACGCACGCATGTTGCTGTGGAACGGTACCCTGCTTGGGGTGATACTGTTGAAGTAGAGTG

CTGGGTTGGTGCATCGGGAAATAATGGCAGGCGCCATGATTTCCTTGTCCGGGACTGCAA

AACAGGCGAAATTCTTACAAGATGTACCAGTCTTTCGGTGATGATGAATACAAGGACAAG

GAGGTTGTCCAAAATCCCTGAAGAAGTTAGAGGGGAGATAGGGCCTGCATTCATTGATAA

TGTGGCTGTCAAGGACGAGGAAATTAAGAAACCACAGAAGCTCAATGACAGCACTGCAG 5 0 ATTAC ATCCAAGGAGGATTGACTCCTCGATGGAATGATTTGGAT ATC AATCAGC ACGTT A

ACAACATCAAATACGTTGACTGGATTCTTGAGACTGTCCCAGACTCAATCTTTGAGAGTCA

TCATATTTCCAGCTTCACTATTGAATACAGGAGAGAGTGCACGAGGGATAGCGTGCTGCA

2018267601 21 Nov 2018

GTCCCTGACCACTGTCTCCGGTGGCTCGTCGGAAGCTGGGTTAGTGTGCGAGCACTTGCTC

CAGCTTGAAGGTGGGTCTGAGGTATTGAGGGCAAAAACAGAGTGGAGGCCTAAGCTTAGT TTCAGAGGGATTAGTGTGATACCCGCAGAATCGAGTGTCTAA

SEQ ID NO:4

Cinnamomum camphor a (Cc) FATB4 amino acid sequence

MVTTSLASAYFSMKAVMLAPDGRGIKPRSSGLQVRAGNERNSCKVINGTKVKDTEGLKGCST LQGQSMLDDHFGLHGLVFRRTFAIRCYEVGPDRSTSIMAVMNHLQEAARNHAESLGLLGDGF GETLEMSKRDLIWWRRTHVAVERYPAWGDTVEVEAWVGASGNTGMRRDFLVRDCKTGHI LTRCTSVSVMMNMRTRRLSKIPQEVRAEIDPLFIEKVAVKEGEIKKLQKLNDSTADYIQGGWT 10 PRWNDLDVNQHVNNIIYVGWIFKSVPDSISENHHLSSITLEYRRECTRGNKLQSLTTVCGGSSE

AGIICEHLLQLEDGSEVLRARTEWRPKHTDSFQGISERFPQQEPHK

SEQ ID NO: 5

Cinnamomum camphora (Cc) FATB4 coding DNA sequence

ATGGTCACCACCTCTTTAGCTTCCGCTTACTTCTCGATGAAAGCTGTAATGTTGGCTCCTGA

CGGCAGGGGCATAAAGCCCAGGAGCAGTGGTTTGCAGGTGAGGGCGGGAAATGAACGAA

ACTCTTGCAAGGTGATCAATGGGACCAAGGTCAAAGACACGGAGGGCTTGAAAGGGTGC

AGCACGTTGCAAGGCCAGAGCATGCTTGATGACCATTTTGGTCTGCATGGGCTAGTTTTCA

GGCGCACCTTTGCAATCAGATGCTATGAGGTTGGACCTGACCGCTCCACATCCATAATGGC TGTTATGAATCACTTGCAGGAAGCTGCACGTAATCATGCGGAGAGTCTGGGACTTCTAGG 20 AGATGGATTCGGTGAGACACTGGAGATGAGTAAGAGAGATCTGATATGGGTTGTGAGACG

CACGCATGTTGCTGTGGAACGGTACCCTGCTTGGGGCGATACTGTTGAAGTCGAGGCCTG

GGTGGGTGCATCAGGTAACACTGGCATGCGCCGCGATTTCCTTGTCCGCGACTGCAAAAC

TGGCCACATTCTTACAAGATGTACCAGTGTTTCAGTGATGATGAATATGAGGACAAGGAG

ATTGTCCAAAATTCCCCAAGAAGTTAGAGCGGAGATTGACCCTCTTTTCATTGAAAAGGTT

GCTGTCAAGGAAGGGGAAATTAAAAAATTACAGAAGTTGAATGATAGCACTGCAGATTAC

ATTCAAGGGGGTTGGACTCCTCGATGGAATGATTTGGATGTCAATCAGCACGTGAACAAT

ATCATATACGTTGGCTGGATTTTTAAGAGCGTCCCAGACTCTATCTCTGAGAATCATCATC

TTTCTAGCATCACTCTCGAATACAGGAGAGAGTGCACAAGGGGCAACAAGCTGCAGTCCC

TGACCACTGTTTGTGGTGGCTCGTCGGAAGCTGGGATCATATGTGAGCACCTACTCCAGCT 3 0 TGAGGATGGGTCTGAGGTTTTGAGGGC AAGAAC AGAGTGGAGGCCC AAGC ACACCGAT A

GTTTCCAAGGCATTAGTGAGAGATTCCCGCAGCAAGAACCGCATAAGTAA

SEQ ID NO: 6

Cinnamomum camphora (Cc) FATB4 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGACCACCTCCCTGGCCTCCGCCTACTTCTCCATGAAGGCCGTGATGCTGGCCCCCG

ACGGCCGCGGCATCAAGCCCCGCTCCTCCGGCCTGCAGGTGCGCGCCGGCAACGAGCGCA

ACTCCTGCAAGGTGATCAACGGCACCAAGGTGAAGGACACCGAGGGCCTGAAGGGCTGC

TCCACCCTGCAGGGCCAGTCCATGCTGGACGACCACTTCGGCCTGCACGGCCTGGTGTTCC

GCCGCACCTTCGCCATCCGCTGCTACGAGGTGGGCCCCGACCGCTCCACCTCCATCATGGC

CGTGATGAACCACCTGCAGGAGGCCGCCCGCAACCACGCCGAGTCCCTGGGCCTGCTGGG

CGACGGCTTCGGCGAGACCCTGGAGATGTCCAAGCGCGACCTGATCTGGGTGGTGCGCCG

CACCCACGTGGCCGTGGAGCGCTACCCCGCCTGGGGCGACACCGTGGAGGTGGAGGCCTG

GGTGGGCGCCTCCGGCAACACCGGCATGCGCCGCGACTTCCTGGTGCGCGACTGCAAGAC

CGGCCACATCCTGACCCGCTGCACCTCCGTGTCCGTGATGATGAACATGCGCACCCGCCGC

CTGTCCAAGATCCCCCAGGAGGTGCGCGCCGAGATCGACCCCCTGTTCATCGAGAAGGTG

GCCGTGAAGGAGGGCGAGATCAAGAAGCTGCAGAAGCTGAACGACTCCACCGCCGACTA

CATCCAGGGCGGCTGGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAA

CATCATCTACGTGGGCTGGATCTTCAAGTCCGTGCCCGACTCCATCTCCGAGAACCACCAC

CTGTCCTCCATCACCCTGGAGTACCGCCGCGAGTGCACCCGCGGCAACAAGCTGCAGTCC

CTGACCACCGTGTGCGGCGGCTCCTCCGAGGCCGGCATCATCTGCGAGCACCTGCTGCAG

CTGGAGGACGGCTCCGAGGTGCTGCGCGCCCGCACCGAGTGGCGCCCCAAGCACACCGAC

TCCTTCCAGGGCATCTCCGAGCGCTTCCCCCAGCAGGAGCCCCACAAGTGA

2018267601 21 Nov 2018

SEQ ID NO: 7

Cinnamomum camphora (Cc) FATB3 amino acid sequence

MVATAAASAFFPVGAPATSSATSAKASMMPDNLDARGIKPKPASSSGLQVKANAHASPKING

SKVSTDTLKGEDTLTSSPAPRTFINQLPDWSMFLAAITTIFLAAEKQWTNLDWKPRRPDMLAD

PFGIGRFMQDGLIFRQHFAIRSYEIGADRTASIETLMNHLQETALNHVRSAGLLGDGFGATPEM

SRRDLIWVVTRMQVLVDRYPAWGDIVEVETWVGASGKNGMRRDWLVRDSQTGEILTRATSV WVMMNKRTRRLSKLPEEVRGEIGPYFIEDVAIIEEDNRKLQKLNENTADNVRRGLTPRWSDLD VNQHVNNVKYIGWILESAPGSILESHELSCMTLEYRRECGKDSVLQSMTAVSGGGSAAGGSPE SSVECDHLLQLESGPEWRGRTEWRPKSANNSRSILEMPAESL

SEQ ID NO: 8

Cinnamomum camphora (Cc) FATB3 coding DNA sequence

ATGGTTGCCACCGCTGCTGCTTCTGCTTTCTTCCCGGTCGGTGCTCCGGCTACGTCATCTGC

AACTTCAGCCAAAGCGTCGATGATGCCTGATAATTTGGATGCCAGAGGCATCAAACCGAA

GCCGGCTTCGTCCAGCGGCTTGCAGGTTAAGGCAAATGCCCATGCCTCTCCCAAGATTAAT 15 GGTTCCAAGGTGAGCACGGATACCTTGAAGGGGGAAGACACCTTAACTTCCTCGCCCGCC

CCACGGACCTTTATCAACCAATTGCCTGACTGGAGCATGTTCCTTGCTGCCATCACAACTA

TTTTCTTGGCTGCCGAGAAGCAGTGGACGAATCTCGACTGGAAGCCCAGAAGACCCGACA

TGCTTGCTGACCCGTTTGGCATCGGGAGGTTTATGCAGGATGGGCTGATTTTCAGGCAGCA

CTTTGCAATCAGATCTTATGAGATTGGGGCTGATAGAACGGCGTCTATAGAGACTTTAATG 20 AATCACTTGCAGGAGACTGCACTTAATCATGTGAGGAGTGCTGGACTCCTAGGTGATGGA

TTTGGTGCGACACCTGAGATGAGTAGAAGAGATCTGATATGGGTTGTAACACGTATGCAG

GTTCTTGTGGACCGCTACCCTGCTTGGGGTGATATTGTTGAAGTAGAGACCTGGGTTGGTG

CATCTGGAAAAAATGGTATGCGCCGTGATTGGCTTGTTCGGGACAGCCAAACTGGTGAAA

TTCTCACACGAGCTACCAGTGTTTGGGTGATGATGAATAAACGGACAAGGCGATTGTCCA 25 AACTTCCTGAAGAAGTTAGAGGGGAAATAGGGCCTTATTTTATAGAAGATGTTGCTATCA

TAGAGGAGGACAACAGGAAACTACAGAAGCTCAATGAAAACACTGCTGATAATGTTCGA

AGGGGTTTGACTCCTCGCTGGAGTGATCTGGATGTTAATCAGCATGTGAACAATGTCAAAT

ACATTGGTTGGATTCTTGAGAGTGCACCAGGATCCATCTTGGAGAGTCATGAGCTTTCCTG

CATGACCCTTGAATACAGGAGAGAATGTGGGAAGGACAGTGTGCTGCAGTCAATGACTGC 3 0 TGTCTCTGGTGGAGGCAGTGC AGC AGGTGGCTC ACC AGAATCT AGCGTTGAGTGTGACC A

CTTGCTCCAGCTAGAGAGTGGGCCTGAAGTTGTGAGGGGAAGAACCGAGTGGAGGCCCA

AGAGTGCTAATAACTCGAGGAGCATCCTGGAGATGCCGGCCGAGAGC

SEQ ID NO: 9

Cinnamomum camphora (Cc) FATB4 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCACCGCCGCCGCCTCCGCCTTCTTCCCCGTGGGCGCCCCCGCCACCTCCTCCG

CCACCTCCGCCAAGGCCTCCATGATGCCCGACAACCTGGACGCCCGCGGCATCAAGCCCA

AGCCCGCCTCCTCCTCCGGCCTGCAGGTGAAGGCCAACGCCCACGCCTCCCCCAAGATCA

ACGGCTCCAAGGTGTCCACCGACACCCTGAAGGGCGAGGACACCCTGACCTCCTCCCCCG 40 CCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGTTCCTGGCCGCCATCACCAC

CATCTTCCTGGCCGCCGAGAAGCAGTGGACCAACCTGGACTGGAAGCCCCGCCGCCCCGA

CATGCTGGCCGACCCCTTCGGCATCGGCCGCTTCATGCAGGACGGCCTGATCTTCCGCCAG

CACTTCGCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGA

TGAACCACCTGCAGGAGACCGCCCTGAACCACGTGCGCTCCGCCGGCCTGCTGGGCGACG 45 GCTTCGGCGCCACCCCCGAGATGTCCCGCCGCGACCTGATCTGGGTGGTGACCCGCATGC

AGGTGCTGGTGGACCGCTACCCCGCCTGGGGCGACATCGTGGAGGTGGAGACCTGGGTGG

GCGCCTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGGTGCGCGACTCCCAGACCGGCG

AGATCCTGACCCGCGCCACCTCCGTGTGGGTGATGATGAACAAGCGCACCCGCCGCCTGT

CCAAGCTGCCCGAGGAGGTGCGCGGCGAGATCGGCCCCTACTTCATCGAGGACGTGGCCA 5 0 TC ATCGAGGAGGAC AACCGCAAGCTGC AGAAGCTGAACGAGAAC ACCGCCGAC AACGTG

CGCCGCGGCCTGACCCCCCGCTGGTCCGACCTGGACGTGAACCAGCACGTGAACAACGTG

AAGTACATCGGCTGGATCCTGGAGTCCGCCCCCGGCTCCATCCTGGAGTCCCACGAGCTGT

CCTGCATGACCCTGGAGTACCGCCGCGAGTGCGGCAAGGACTCCGTGCTGCAGTCCATGA

WO 2014/151904

PCT/US2014/026644

2018267601 21 Nov 2018

CCGCCGTGTCCGGCGGCGGCTCCGCCGCCGGCGGCTCCCCCGAGTCCTCCGTGGAGTGCG

ACCACCTGCTGCAGCTGGAGTCCGGCCCCGAGGTGGTGCGCGGCCGCACCGAGTGGCGCC CCAAGTCCGCCAACAACTCCCGCTCCATCCTGGAGATGCCCGCCGAGTCCCTGTGA

SEQ ID NO: 10

Cuphea hyssopifolia (Chs) FATB1 amino acid sequence

MVATNAAAFSAYTFFLTSPTHGYSSKRLADTQNGYPGTSLKSKSTPPPAAAAARNGALPLLAS ICKCPKKADGSMQLDSSLVFGFQFYIRSYEVGADQTVSIQTVLNYLQEAAINHVQSAGYFGDS FGATPEMTKRNLIWVITKMQVLVDRYPAWGDVVQVDTWTCSSGKNSMQRDWFVRDLKTGD IITRASSVWVLMNRLTRKLSKIPEAVLEEAKLFVMNTAPTVDDNRKLPKLDGSSADYVLSGLT 10 PRWSDLDMNQHVNNVKYIAWILESVPQSIPETHKLSAITVEYRRECGKNSVLQSLTNVSGDGI

TCGNSIIECHHLLQLETGPEILLARTEWISKEPGFRGAPIQAEKVYNNK*

SEQ ID NO: 11

Cuphea hyssopifolia (Chs) FATB1 coding DNA sequence

ATGGTTGCCACTAATGCTGCTGCCTTTTCTGCTTATACTTTCTTCCTTACTTCACCAACTCA

TGGTTACTCTTCCAAACGTCTCGCCGATACTCAAAATGGTTATCCGGGTACCTCCTTGAAA

TCGAAATCCACTCCTCCACCAGCTGCTGCTGCTGCTCGTAACGGTGCATTGCCACTGCTGG

CCTCCATCTGCAAATGCCCCAAAAAGGCTGATGGGAGTATGCAACTAGACAGCTCCTTGG

TCTTCGGGTTTCAATTTTACATTAGATCATATGAAGTGGGTGCGGATCAAACCGTGTCAAT ACAGACAGTACTCAATTACTTACAGGAGGCAGCCATCAATCATGTTCAGAGTGCTGGCTA 20 TTTTGGTGATAGTTTTGGCGCCACCCCGGAAATGACCAAGAGGAACCTCATCTGGGTTATC

ACTAAGATGCAGGTTTTGGTGGATCGCTATCCCGCTTGGGGCGATGTTGTTCAAGTTGATA CATGGACCTGTAGTTCTGGTAAAAACAGCATGCAGCGTGATTGGTTCGTACGGGATCTCA

AAACTGGAGATATTATAACAAGAGCCTCGAGCGTGTGGGTGCTGATGAATAGACTCACCA

GAAAATTATCAAAAATTCCTGAAGCAGTTCTGGAAGAAGCAAAACTTTTTGTGATGAACA

CTGCCCCCACCGTAGATGACAACAGGAAGCTACCAAAGCTGGATGGCAGCAGTGCTGATT

ATGTCCTCTCTGGCTTAACTCCTAGATGGAGCGACTTAGATATGAACCAGCATGTCAACAA

TGTGAAGTACATAGCCTGGATCCTTGAGAGTGTCCCTCAGAGCATACCGGAGACACACAA

GCTGTCAGCGATAACCGTGGAGTACAGGAGAGAATGTGGCAAGAACAGCGTCCTCCAGTC TCTGACCAACGTCTCCGGGGATGGAATCACATGTGGAAACAGTATTATCGAGTGCCACCA 3 0 TTTGCTTC AACTTGAGACTGGCCCAGAGATTCTACTAGCGCGGACGGAGTGGAT ATCC AA

GGAACCTGGGTTCAGGGGAGCTCCAATCCAGGCAGAGAAAGTCTACAACAACAAATAA

SEQ ID NO: 12

Cuphea hyssopifolia (Chs) FATB1 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCACCAACGCCGCCGCCTTCTCCGCCTACACCTTCTTCCTGACCTCCCCCACCC

5 ACGGCT ACTCCTCC AAGCGCCTGGCCGAC ACCC AGAACGGCTACCCCGGC ACCTCCCTGA

AGTCCAAGTCCACCCCCCCCCCCGCCGCCGCCGCCGCCCGCAACGGCGCCCTGCCCCTGCT

GGCCTCCATCTGCAAGTGCCCCAAGAAGGCCGACGGCTCCATGCAGCTGGACTCCTCCCT

GGTGTTCGGCTTCCAGTTCTACATCCGCTCCTACGAGGTGGGCGCCGACCAGACCGTGTCC

ATCCAGACCGTGCTGAACTACCTGCAGGAGGCCGCCATCAACCACGTGCAGTCCGCCGGC

TACTTCGGCGACTCCTTCGGCGCCACCCCCGAGATGACCAAGCGCAACCTGATCTGGGTG

ATCACCAAGATGCAGGTGCTGGTGGACCGCTACCCCGCCTGGGGCGACGTGGTGCAGGTG

GACACCTGGACCTGCTCCTCCGGCAAGAACTCCATGCAGCGCGACTGGTTCGTGCGCGAC

CTGAAGACCGGCGACATCATCACCCGCGCCTCCTCCGTGTGGGTGCTGATGAACCGCCTG

ACCCGCAAGCTGTCCAAGATCCCCGAGGCCGTGCTGGAGGAGGCCAAGCTGTTCGTGATG

AACACCGCCCCCACCGTGGACGACAACCGCAAGCTGCCCAAGCTGGACGGCTCCTCCGCC

GACTACGTGCTGTCCGGCCTGACCCCCCGCTGGTCCGACCTGGACATGAACCAGCACGTG

AACAACGTGAAGTACATCGCCTGGATCCTGGAGTCCGTGCCCCAGTCCATCCCCGAGACC

CACAAGCTGTCCGCCATCACCGTGGAGTACCGCCGCGAGTGCGGCAAGAACTCCGTGCTG

CAGTCCCTGACCAACGTGTCCGGCGACGGCATCACCTGCGGCAACTCCATCATCGAGTGC

CACCACCTGCTGCAGCTGGAGACCGGCCCCGAGATCCTGCTGGCCCGCACCGAGTGGATC

TCCAAGGAGCCCGGCTTCCGCGGCGCCCCCATCCAGGCCGAGAAGGTGTACAACAACAAG TGA

2018267601 21 Nov 2018

SEQ ID NO: 13

Cuphea hyssopifolia (Chs) FATB2 amino acid sequence

MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAPPKINGSSVG LKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDP

FGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFGRTLEM YKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASS VWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTPKWNDL DVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKGSGSQFQ HLLRLEDGGEIVKGRTEWRPKTAGINGPIASGETSPGDSS*

SEQ ID NO: 14

Cuphea hyssopifolia (Chs) FATB2 coding DNA sequence

ATGGTGGCTACCGCTGCAAGTTCAGCATTCTTCCCTGTGCCGTCCCCCGACGCCTCCTCTA GACCTGGAAAGCTCGGCAATGGGTCATCGAGCTTGAGCCCCCTCAAGCCCAAATTGATGG

CCAATGGCGGGTTGCAGGTTAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTCTT

CGGTCGGTCTAAAGTCCGGCAGTCTCAAGACTCAGGAAGACACTCCTTCGGCGCCTCCTCC CCGGACTTTTATTAACCAGCTGCCTGATTGGAGTATGCTTCTTGCTGCAATCACTACTGTCT TCTTGGCAGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCCAAGAGGCCTGACATGC TTGTGGACCCGTTCGGATTGGGAAGGATTGTTCAAGATGGGCTTGTGTTCAGGCAGAATTT TTCGATTAGGTCCTATGAAATAGGCGCTGATCGCACTGCGTCTATAGAGACGGTGATGAA

CCACTTGCAGGAAACAGCTCTCAATCATGTTAAGAGTGCTGGGCTTCTTAATGACGGCTTT

GGTCGTACTCTTGAGATGTATAAAAGGGACCTTATTTGGGTTGTTGCAAAAATGCAGGTCA TGGTTAACCGCTATCCTACTTGGGGCGACACGGTTGAAGTGAATACTTGGGTTGCCAAGTC

AGGGAAAAATGGTATGCGTCGTGATTGGCTCATAAGTGATTGCAATACAGGAGAAATTCT TACTAGAGCATCAAGTGTGTGGGTCATGATGAATCAAAAGACAAGAAGATTGTCAAAAAT

TCCAGATGAGGTTCGACATGAGATAGAGCCTCATTTCGTGGACTCTGCTCCCGTCATTGAA GATGATGACCGGAAACTTCCCAAGCTGGATGAGAAGACTGCTGACTCCATCCGCAAGGGT CTAACTCCGAAGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTACATT GGGTGGATTCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGTTCCCTTA CCCTGGAATATAGGCGGGAATGCGGAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTGTGG

0 ACCCCTCTGGAAAGGGCTCTGGGTCTC AGTTCC AGC ACCTTCTGCGGCTTGAGGATGGAG

GTGAGATTGTGAAGGGGAGAACTGAGTGGCGACCCAAGACTGCAGGAATCAATGGGCCA ATAGCATCCGGGGAGACCTCACCTGGAGACTCTTCTTAG

SEQ ID NO: 15

Cuphea hyssopifolia (Chs) FATB2 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCCCCGACGCCTCCTCCCG CCCCGGCAAGCTGGGCAACGGCTCCTCCTCCCTGTCCCCCCTGAAGCCCAAGCTGATGGCC AACGGCGGCCTGCAGGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCC GTGGGCCTGAAGTCCGGCTCCCTGAAGACCCAGGAGGACACCCCCTCCGCCCCCCCCCCC CGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGGCCGCCATCACCACCGTGT

TCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAAGCGCCCCGACATGC TGGTGGACCCCTTCGGCCTGGGCCGCATCGTGCAGGACGGCCTGGTGTTCCGCCAGAACTT CTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAA CCACCTGCAGGAGACCGCCCTGAACCACGTGAAGTCCGCCGGCCTGCTGAACGACGGCTT CGGCCGCACCCTGGAGATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGT

GATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCCAA

GTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGAT CCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGACCCGCCGCCTGTCCAA

GATCCCCGACGAGGTGCGCCACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCCGTGAT

CGAGGACGACGACCGCAAGCTGCCCAAGCTGGACGAGAAGACCGCCGACTCCATCCGCA

0 AGGGCCTGACCCCC AAGTGGAACGACCTGGACGTGAACC AGC ACGTGAAC AACGTGAAG

TACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGC

TCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACC

GCCGTGGACCCCTCCGGCAAGGGCTCCGGCTCCCAGTTCCAGCACCTGCTGCGCCTGGAG

2018267601 21 Nov 2018

GACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGACCGCCGGCATCAA CGGCCCCATCGCCTCCGGCGAGACCTCCCCCGGCGACTCCTCCTGA

SEQ ID NO: 16

Cuphea hyssopifolia (Chs) FATB2b +a.a.248-259 variant amino acid sequence

MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAPPKINGSSVG LKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDP FGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFGRTLEM YKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASS KSQIMLPLHYCSVWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADS

IRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTA VDPSGKGSGSQFQHLLRLEDGGEIVKGRTEWRPKTAGINGPIASGETSPGDSS*

SEQ ID NO: 17

Cuphea hyssopifolia (Chs) FATB2b+a.a.248-259 variant coding DNA sequence

ATGGTGGCTACCGCTGCAAGTTCAGCATTCTTCCCTGTGCCGTCCCCCGACGCCTCCTCTA

GACCTGGAAAGCTCGGCAATGGGTCATCGAGCTTGAGCCCCCTCAAGCCCAAATTGATGG

CCAATGGCGGGTTGCAGGTTAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTCTT

CGGTCGGTCTAAAGTCCGGCAGTCTCAAGACTCAGGAAGACACTCCTTCGGCGCCTCCTCC CCGGACTTTTATTAACCAGCTGCCTGATTGGAGTATGCTTCTTGCTGCAATCACTACTGTCT

TCTTGGCAGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCCAAGAGGCCTGACATGC

TTGTGGACCCGTTCGGATTGGGAAGGATTGTTCAAGATGGGCTTGTGTTCAGGCAGAATTT TTCGATTAGGTCCTATGAAATAGGCGCTGATCGCACTGCGTCTATAGAGACGGTGATGAA CCACTTGCAGGAAACAGCTCTCAATCATGTTAAGAGTGCTGGGCTTCTTAATGACGGCTTT

GGTCGTACTCTTGAGATGTATAAAAGGGACCTTATTTGGGTTGTTGCAAAAATGCAGGTCA TGGTTAACCGCTATCCTACTTGGGGCGACACGGTTGAAGTGAATACTTGGGTTGCCAAGTC

AGGGAAAAATGGTATGCGTCGTGATTGGCTCATAAGTGATTGCAATACAGGAGAAATTCT

TACTAGAGCATCAAGTAAAAGCCAAATTATGTTACCCTTACATTATTGCAGTGTGTGGGTC

ATGATGAATCAAAAGACAAGAAGATTGTCAAAAATTCCAGATGAGGTTCGACATGAGATA GAGCCTCATTTCGTGGACTCTGCTCCCGTCATTGAAGATGATGACCGGAAACTTCCCAAGC

TGGATGAGAAGACTGCTGACTCCATCCGCAAGGGTCTAACTCCGAAGTGGAATGACTTGG

0 ATGTC AATCAGCACGTCAACAACGTGAAGTACATTGGGTGGATTCTTGAGAGT ACTCC AC

CAGAAGTTCTGGAGACCCAGGAGTTATGTTCCCTTACCCTGGAATATAGGCGGGAATGCG

GAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTGTGGACCCCTCTGGAAAGGGCTCTGGGT

CTCAGTTCCAGCACCTTCTGCGGCTTGAGGATGGAGGTGAGATTGTGAAGGGGAGAACTG AGTGGCGACCCAAGACTGCAGGAATCAATGGGCCAATAGCATCCGGGGAGACCTCACCTG

GAGACTCTTCTTAG

SEQ ID NO: 18

Cuphea hyssopifolia (Chs) FATB2b +a.a.248-259 variant coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCCCCGACGCCTCCTCCCG

CCCCGGCAAGCTGGGCAACGGCTCCTCCTCCCTGTCCCCCCTGAAGCCCAAGCTGATGGCC

AACGGCGGCCTGCAGGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCC GTGGGCCTGAAGTCCGGCTCCCTGAAGACCCAGGAGGACACCCCCTCCGCCCCCCCCCCC

CGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGGCCGCCATCACCACCGTGT TCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAAGCGCCCCGACATGC

TGGTGGACCCCTTCGGCCTGGGCCGCATCGTGCAGGACGGCCTGGTGTTCCGCCAGAACTT CTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAA CCACCTGCAGGAGACCGCCCTGAACCACGTGAAGTCCGCCGGCCTGCTGAACGACGGCTT

CGGCCGCACCCTGGAGATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGT

GATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCCAA

0 GTCCGGC AAGAACGGCATGCGCCGCGACTGGCTGATCTCCGACTGC AAC ACCGGCGAGAT

CCTGACCCGCGCCTCCTCCAAGTCCCAGATCATGCTGCCCCTGCACTACTGCTCCGTGTGG

GTGATGATGAACCAGAAGACCCGCCGCCTGTCCAAGATCCCCGACGAGGTGCGCCACGAG

2018267601 21 Nov 2018

ATCGAGCCCCACTTCGTGGACTCCGCCCCCGTGATCGAGGACGACGACCGCAAGCTGCCC

AAGCTGGACGAGAAGACCGCCGACTCCATCCGCAAGGGCCTGACCCCCAAGTGGAACGA

CCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGATCCTGGAGTCCAC

CCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGCCGCGA

GTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCGCCGTGGACCCCTCCGGCAAGGGCTC

CGGCTCCCAGTTCCAGCACCTGCTGCGCCTGGAGGACGGCGGCGAGATCGTGAAGGGCCG CACCGAGTGGCGCCCCAAGACCGCCGGCATCAACGGCCCCATCGCCTCCGGCGAGACCTC CCCCGGCGACTCCTCCTGA

SEQ ID NO: 19

Cuphea hyssopifolia (Chs) FATB3 amino acid sequence

MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASARPKANGSA

VSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLM DPFGVDRWQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEM CKRDLIWWTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDCHTGEILIRATSMC 15 AMMNQKTRRF SKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRWNDLDV

NQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRRECGRDSVLESVTAMDPSKEGDRSLYQH LLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS*

SEQ ID NO: 20

Cuphea hyssopifolia (Chs) FATB3 coding DNA sequence

ATGGTGGCTGCCGAAGCAAGTTCTGCACTCTTCTCCGTTCGAACCCCGGGAACCTCCCCTA

AACCCGGGAAGTTCGGGAATTGGCCAACGAGCTTGAGCGTCCCCTTCAAGTCCAAATCAA ACCACAATGGCGGCTTTCAGGTTAAGGCAAACGCCAGTGCCCGTCCTAAGGCTAACGGTT

CTGCAGTAAGTCTAAAGTCTGGCAGCCTCGACACTCAGGAGGACACTTCATCGTCGTCCTC TCCTCCTCGGACTTTCATTAACCAGTTGCCCGACTGGAGTATGCTGCTGTCCGCGATCACG 25 ACCGTCTTCGTGGCGGCTGAGAAGCAGTGGACGATGCTTGATCGGAAATCTAAGAGGCCC

GACATGCTCATGGACCCGTTTGGGGTTGACAGGGTTGTTCAGGATGGGGCTGTGTTCAGA

CAGAGTTTTTCGATTAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAGACG

CTGATGAACATCTTCCAGGAAACATCTCTCAATCATTGTAAGAGTATCGGTCTTCTCAATG

ACGGCTTTGGTCGTACTCCTGAGATGTGTAAGAGGGACCTCATTTGGGTGGTTACAAAAAT 3 0 GC ACGTCGAGGTTAATCGCTATCCTACTTGGGGTGATACTATCGAGGTCAAT ACTTGGGTC

TCCGAGTCGGGGAAAACCGGTATGGGTCGTGATTGGCTGATAAGTGATTGTCATACAGGA GAAATTCTAATAAGAGCAACGAGCATGTGTGCTATGATGAATCAAAAGACGAGAAGATTC

TCAAAATTTCCATATGAGGTTCGACAGGAGTTGGCGCCTCATTTTGTGGACTCTGCTCCTG TCATTGAAGACTATCAAAAATTGCACAAGCTTGATGTGAAGACGGGTGATTCCATTTGCA 3 5 ATGGCCT AACTCC AAGGTGGAATGACTTGGATGTC AATC AGC ACGTTAAC AATGTGAAGT

ACATTGGGTGGATTCTCGAGAGTGTTCCAACGGAAGTTTTCGAGACCCAGGAGCTATGTG

GCCTCACCCTTGAGTATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCCGTGACCG

CTATGGATCCATCAAAAGAGGGAGACAGATCTCTGTACCAGCACCTTCTTCGGCTTGAGG

ATGGGGCTGATATCGCGAAGGGCAGAACCAAGTGGCGGCCGAAGAATGCAGGAACCAAT 40 GGGGCAATATCAACAGGAAAGACTTCAAATGGAAACTCGATCTCTTAG

SEQ ID NO: 21

Cuphea hyssopifolia (Chs) FATB3 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCGAGGCCTCCTCCGCCCTGTTCTCCGTGCGCACCCCCGGCACCTCCCCCA

AGCCCGGCAAGTTCGGCAACTGGCCCACCTCCCTGTCCGTGCCCTTCAAGTCCAAGTCCAA 45 CCACAACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCGCCCCAAGGCCAACGGCTC

CGCCGTGTCCCTGAAGTCCGGCTCCCTGGACACCCAGGAGGACACCTCCTCCTCCTCCTCC

CCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGTCCGCCATCACCA

CCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCG

ACATGCTGATGGACCCCTTCGGCGTGGACCGCGTGGTGCAGGACGGCGCCGTGTTCCGCC 50 AGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCT

GATGAACATCTTCCAGGAGACCTCCCTGAACCACTGCAAGTCCATCGGCCTGCTGAACGA

CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGAT

2018267601 21 Nov 2018

GCACGTGGAGGTGAACCGCTACCCCACCTGGGGCGACACCATCGAGGTGAACACCTGGGT

GTCCGAGTCCGGCAAGACCGGCATGGGCCGCGACTGGCTGATCTCCGACTGCCACACCGG CGAGATCCTGATCCGCGCCACCTCCATGTGCGCCATGATGAACCAGAAGACCCGCCGCTT

CTCCAAGTTCCCCTACGAGGTGCGCCAGGAGCTGGCCCCCCACTTCGTGGACTCCGCCCCC

GTGATCGAGGACTACCAGAAGCTGCACAAGCTGGACGTGAAGACCGGCGACTCCATCTGC

AACGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAA

GTACATCGGCTGGATCCTGGAGTCCGTGCCCACCGAGGTGTTCGAGACCCAGGAGCTGTG

CGGCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGAC

CGCCATGGACCCCTCCAAGGAGGGCGACCGCTCCCTGTACCAGCACCTGCTGCGCCTGGA

GGACGGCGCCGACATCGCCAAGGGCCGCACCAAGTGGCGCCCCAAGAACGCCGGCACCA ACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCATCTCCTGA

SEQ ID NO: 22

Cuphea hyssopifolia (Chs) FATB3b (V204I,C239F, E243D, M251V variant) amino acid sequence

MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASARPKANGSA

VSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLM DPFGVDRWQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEM CKRDLIWWTKMHIEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDFHTGDILIRATSVC AMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRWNDLDV NQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRRECGRDSVLESVTAMDPSKEGDRSLYQH

LLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS*

SEQ ID NO: 23

Cuphea hyssopifolia (Chs) FATB3b (V204I,C239F, E243D, M251V variant) coding DNA sequence

ATGGTGGCTGCCGAAGCAAGTTCTGCACTCTTCTCCGTTCGAACCCCGGGAACCTCCCCTA AACCCGGGAAGTTCGGGAATTGGCCAACGAGCTTGAGCGTCCCCTTCAAGTCCAAATCAA

ACCACAATGGCGGCTTTCAGGTTAAGGCAAACGCCAGTGCCCGTCCTAAGGCTAACGGTT

CTGCAGTAAGTCTAAAGTCTGGCAGCCTCGACACTCAGGAGGACACTTCATCGTCGTCCTC TCCTCCTCGGACTTTCATTAACCAGTTGCCCGACTGGAGTATGCTGCTGTCCGCGATCACG

ACCGTCTTCGTGGCGGCTGAGAAGCAGTGGACGATGCTTGATCGGAAATCTAAGAGGCCC GACATGCTCATGGACCCGTTTGGGGTTGACAGGGTTGTTCAGGATGGGGCTGTGTTCAGA

0 C AGAGTTTTTCGATTAGGTCTT ACGAAATAGGCGCTGATCGAACAGCCTCT AT AGAGACG

CTGATGAACATCTTCCAGGAAACATCTCTCAATCATTGTAAGAGTATCGGTCTTCTCAATG ACGGCTTTGGTCGTACTCCTGAGATGTGTAAGAGGGACCTCATTTGGGTGGTTACAAAAAT

GCACATCGAGGTTAATCGCTATCCTACTTGGGGTGATACTATCGAGGTCAATACTTGGGTC TCCGAGTCGGGGAAAACCGGTATGGGTCGTGATTGGCTGATAAGTGATTTTCATACAGGA

5 GAC ATTCTAAT AAGAGC AACGAGCGTGTGTGCT ATGATGAATC AAAAGACGAGAAGATTC

TCAAAATTTCCATATGAGGTTCGACAGGAGTTAGCGCCTCATTTTGTGGACTCTGCTCCAG TCATTGAAGACTATCAAAAATTGCACAAGCTTGATGTGAAGACGGGTGATTCCATTTGCA

ATGGCCTAACTCCAAGGTGGAATGACTTGGATGTCAATCAGCACGTTAACAATGTGAAGT ACATTGGGTGGATTCTCGAGAGTGTTCCAACGGAAGTTTTCGAGACCCAGGAGCTATGTG

GCCTCACCCTTGAGTATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCCGTGACCG

CTATGGATCCCTCAAAAGAGGGAGACAGATCTCTGTACCAGCACCTTCTTCGGCTTGAGG ATGGGGCTGATATCGCGAAGGGCAGAACCAAGTGGCGGCCGAAGAATGCAGGAACCAAT

GGGGCAATATCAACAGGAAAGACTTCAAATGGAAACTCGATCTCTTAG

SEQ ID NO: 24

Cuphea hyssopifolia (Chs) FATB3b (V204I,C239F, E243D, M251V variant) coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCGAGGCCTCCTCCGCCCTGTTCTCCGTGCGCACCCCCGGCACCTCCCCCA

AGCCCGGCAAGTTCGGCAACTGGCCCACCTCCCTGTCCGTGCCCTTCAAGTCCAAGTCCAA

CCACAACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCGCCCCAAGGCCAACGGCTC

CGCCGTGTCCCTGAAGTCCGGCTCCCTGGACACCCAGGAGGACACCTCCTCCTCCTCCTCC

CCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGTCCGCCATCACCA

CCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCG

2018267601 21 Nov 2018

ACATGCTGATGGACCCCTTCGGCGTGGACCGCGTGGTGCAGGACGGCGCCGTGTTCCGCC

AGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCT

GATGAACATCTTCCAGGAGACCTCCCTGAACCACTGCAAGTCCATCGGCCTGCTGAACGA CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGAT

GCACATCGAGGTGAACCGCTACCCCACCTGGGGCGACACCATCGAGGTGAACACCTGGGT

GTCCGAGTCCGGCAAGACCGGCATGGGCCGCGACTGGCTGATCTCCGACTTCCACACCGG CGACATCCTGATCCGCGCCACCTCCGTGTGCGCCATGATGAACCAGAAGACCCGCCGCTT

CTCCAAGTTCCCCTACGAGGTGCGCCAGGAGCTGGCCCCCCACTTCGTGGACTCCGCCCCC

GTGATCGAGGACTACCAGAAGCTGCACAAGCTGGACGTGAAGACCGGCGACTCCATCTGC

AACGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAA

GTACATCGGCTGGATCCTGGAGTCCGTGCCCACCGAGGTGTTCGAGACCCAGGAGCTGTG

CGGCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGAC

CGCCATGGACCCCTCCAAGGAGGGCGACCGCTCCCTGTACCAGCACCTGCTGCGCCTGGA

GGACGGCGCCGACATCGCCAAGGGCCGCACCAAGTGGCGCCCCAAGAACGCCGGCACCA

ACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCATCTCCTGA

SEQ ID NO: 25

Cuphea PSR23 (Cu) FATB3 amino acid sequence

MWAAATSAFFPVPAPGTSPKPGKSGNWPSSLSPTFKPKSIPNAGFQVKANASAHPKANGSAV NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRPDMLVD

SVGLKCIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGM CKNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSQSGKIGMASDWLISDCNTGEILIRATSVW AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDQKLHKFDVKTGDSIRKGLTPRWNDLD VNQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAVDPSENGGRSQYK HLLRLEDGTDIVKSRTEWRPKNAGTNGAISTSTAKTSNGNSVS*

SEQ ID NO: 26

Cuphea PSR23 (Cu) FATB3 coding DNA sequence

ATGGTGGTGGCTGCAGCAACTTCTGCATTCTTCCCCGTTCCAGCCCCGGGAACCTCCCCTA

AACCCGGGAAGTCCGGCAACTGGCCATCGAGCTTGAGCCCTACCTTCAAGCCCAAGTCAA

TCCCCAATGCCGGATTTCAGGTTAAGGCAAATGCCAGTGCCCATCCTAAGGCTAACGGTTC 3 0 TGCAGTAAATCT AAAGTCTGGC AGCCTC AACACTC AGGAGGACACTTCGTCGTCCCCTCCT

CCCCGGGCTTTCCTTAACCAGTTGCCTGATTGGAGTATGCTTCTGACTGCAATCACGACCG

TCTTCGTGGCGGCAGAGAAGCAGTGGACTATGCTTGATAGGAAATCTAAGAGGCCTGACA TGCTCGTGGACTCGGTTGGGTTGAAGTGTATTGTTCGGGATGGGCTCGTGTCCAGACAGAG _{TTTTTTGATTAGATCTTATGAAATAGGCGCTGATCGAACAGCCTCTATAGAGACGCTGATG}

5 AACC ACTTGCAGGAAAC ATCTATC AATCATTGT AAGAGTTTGGGTCTTCTCAATGACGGCT

TTGGTCGTACTCCTGGGATGTGTAAAAACGACCTCATTTGGGTGCTTACAAAAATGCAGAT

CATGGTGAATCGCTACCCAACTTGGGGCGATACTGTTGAGATCAATACCTGGTTCTCTCAG

TCGGGGAAAATCGGTATGGCTAGCGATTGGCTAATAAGTGATTGCAACACAGGAGAAATT CTTATAAGAGCAACGAGCGTGTGGGCTATGATGAATCAAAAGACGAGAAGATTCTCAAGA

CTTCCATACGAGGTTCGCCAGGAGTTAACGCCTCATTTTGTGGACTCTCCTCATGTCATTG

AAGACAATGATCAGAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTCGCAAGG

GTCTAACTCCGAGGTGGAACGACTTGGATGTGAATCAGCACGTAAGCAACGTGAAGTACA

TTGGGTGGATTCTCGAGAGTATGCCAATAGAAGTTTTGGAGACACAGGAGCTATGCTCTCT CACCGTAGAATATAGGCGGGAATGCGGAATGGACAGTGTGCTGGAGTCCGTGACTGCTGT

GGATCCCTCAGAAAATGGAGGCCGGTCTCAGTACAAGCACCTTCTGCGGCTTGAGGATGG

GACTGATATCGTGAAGAGCAGAACTGAGTGGCGACCGAAGAATGCAGGAACTAACGGGG CGATATCAACATCAACAGCAAAGACTTCAAATGGAAACTCGGTCTCTTAG

SEQ ID NO: 27

Cuphea PSR23 (Cu) FATB3 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGTGGCCGCCGCCACCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCTCCCCCA

AGCCCGGCAAGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCACCTTCAAGCCCAAGTCCAT

CCCCAACGCCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC

2018267601 21 Nov 2018

CGCCGTGAACCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCC

CCCCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGACCGCCATCACCACCG TGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCGACA

TGCTGGTGGACTCCGTGGGCCTGAAGTGCATCGTGCGCGACGGCCTGGTGTCCCGCCAGT

CCTTCCTGATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGAT GAACCACCTGCAGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGG CTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCA GATCATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACACCTGGTTCTC CCAGTCCGGCAAGATCGGCATGGCCTCCGACTGGCTGATCTCCGACTGCAACACCGGCGA

GATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCTCC

CGCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTTCGTGGACTCCCCCCACGTG

ATCGAGGACAACGACCAGAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCCGC

AAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAG TACATCGGCTGGATCCTGGAGTCCATGCCCATCGAGGTGCTGGAGACCCAGGAGCTGTGC

TCCCTGACCGTGGAGTACCGCCGCGAGTGCGGCATGGACTCCGTGCTGGAGTCCGTGACC

GCCGTGGACCCCTCCGAGAACGGCGGCCGCTCCCAGTACAAGCACCTGCTGCGCCTGGAG GACGGCACCGACATCGTGAAGTCCCGCACCGAGTGGCGCCCCAAGAACGCCGGCACCAA CGGCGCCATCTCCACCTCCACCGCCAAGACCTCCAACGGCAACTCCGTGTCCTGA

SEQ ID NO: 28

Cuphea wrightii (Cw) FATB3 amino acid sequence

MWAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSPHPKANGSA VSLKSGSLNTLEDPPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQFTRLDRKSKRPDMLVDW FGSETIVQDGLVFRERFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTSEMC TRDLIWVLTKMQIWNRYPTWGDTVEINSWFSQSGKIGMGRDWLISDCNTGEILVRATSAWA

MMNQKTRRFSKLPCEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPGWNDLDV NQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRRECGRESVVESVTSMNPSKVGDRSQYQH LLRLEDGADIMKGRTEWRPKNAGTNRAIST*

SEQ ID NO: 29

Cuphea wrightii (Cw) FATB3 coding DNA sequence

0 ATGGTGGTGGCTGCTGC AGCAAGTTCTGC ATTCTTCCCTGTTCC AGC ACCTAGAACC ACGC

CTAAACCCGGGAAGTTCGGCAATTGGCCATCGAGCTTGAGCCCGCCCTTCAAGCCCAAGT CAAACCCCAATGGTAGATTTCAGGTTAAGGCAAATGTCAGTCCTCATCCTAAGGCTAACG GTTCTGCAGTAAGTCTAAAGTCTGGCAGCCTCAACACTCTGGAGGACCCTCCGTCGTCCCC TCCTCCTCGGACTTTCCTTAACCAGTTGCCTGATTGGAGTAGGCTTCGGACTGCAATCACG

5 ACCGTCTTCGTGGCGGCAGAGAAGC AGTTC ACTAGGCTCGATCGAAAATCT AAGAGGCCT

GACATGCTAGTGGACTGGTTTGGGTCAGAGACTATTGTTCAGGATGGGCTCGTGTTCAGA GAGAGATTTTCGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAGACG

CTGATGAACCACTTGCAGGACACATCTCTGAATCATTGTAAGAGTGTGGGTCTTCTCAATG ACGGCTTTGGTCGTACCTCGGAGATGTGTACAAGAGACCTCATTTGGGTGCTTACAAAAAT

GCAGATCGTGGTGAATCGCTATCCAACTTGGGGCGATACTGTCGAGATCAATAGCTGGTT

CTCCCAGTCGGGGAAAATCGGTATGGGTCGCGATTGGCTAATAAGTGATTGCAACACAGG AGAAATTCTTGTAAGAGCAACGAGCGCTTGGGCCATGATGAATCAAAAGACGAGAAGATT

CTCAAAACTTCCATGCGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCTCCTCCT GTCATTGAAGACAATGATCGGAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTT

GCAAGGGTCTAACTCCGGGGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGA AGTACATTGGGTGGATTCTCGAGAGTATGCCTACAGAAGTTTTGGAGACCCAGGAGCTAT GCTCTCTCACCCTTGAATATAGGCGGGAATGTGGAAGGGAAAGTGTGGTAGAGTCCGTGA

CCTCTATGAATCCCTCAAAAGTTGGAGACCGGTCTCAGTACCAACACCTTCTGCGGCTTGA GGATGGGGCTGATATCATGAAGGGCAGAACTGAGTGGAGACCAAAGAATGCAGGAACCA

ACCGGGCGATATCAACATGA

SEQ ID NO: 30

Cuphea wrightii (Cw) FATB3 coding DNA sequence codon optimized for Prototheca moriformis

2018267601 21 Nov 2018

ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCCCCCGCACCACCC

CCAAGCCCGGCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCCCCCCCTTCAAGCCCAAGTC CAACCCCAACGGCCGCTTCCAGGTGAAGGCCAACGTGTCCCCCCACCCCAAGGCCAACGG

CTCCGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCCTGGAGGACCCCCCCTCCTCCCCC

CCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGTCCCGCCTGCGCACCGCCATCACCA CCGTGTTCGTGGCCGCCGAGAAGCAGTTCACCCGCCTGGACCGCAAGTCCAAGCGCCCCG ACATGCTGGTGGACTGGTTCGGCTCCGAGACCATCGTGCAGGACGGCCTGGTGTTCCGCG AGCGCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCT GATGAACCACCTGCAGGACACCTCCCTGAACCACTGCAAGTCCGTGGGCCTGCTGAACGA

CGGCTTCGGCCGCACCTCCGAGATGTGCACCCGCGACCTGATCTGGGTGCTGACCAAGAT

GCAGATCGTGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACTCCTGGTT CTCCCAGTCCGGCAAGATCGGCATGGGCCGCGACTGGCTGATCTCCGACTGCAACACCGG

CGAGATCCTGGTGCGCGCCACCTCCGCCTGGGCCATGATGAACCAGAAGACCCGCCGCTT CTCCAAGCTGCCCTGCGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCCCCCCC

CGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCAT

CTGCAAGGGCCTGACCCCCGGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGT GAAGTACATCGGCTGGATCCTGGAGTCCATGCCCACCGAGGTGCTGGAGACCCAGGAGCT GTGCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGGTGGAGTCCGT GACCTCCATGAACCCCTCCAAGGTGGGCGACCGCTCCCAGTACCAGCACCTGCTGCGCCT

GGAGGACGGCGCCGACATCATGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA CCAACCGCGCCATCTCCACCTGA

SEQ ID NO: 31

Cuphea wrightii (Cw) FATB4a amino acid sequence

MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPPKINGSSVGL

KSGGFKTQEDSPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPF

GLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSNDGFGRTPEMYK RDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSVW VMMNQKTRRLSKIPDEVRNEIEPHFVDSAPWEDDDRKLPKLDENTADSIRKGLTPRWNDLD VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSAEGYASRFQH 30 LLRLEDGGEIVKARTEWRPKNAGINGWPSEESSPGDFF*

SEQ ID NO: 32

Cuphea wrightii (Cw) FATB4a coding DNA sequence

TTGGTGGCTACCGCTGCAAGTTCTGCATTTTTCCCCGTGCCATCCGCCGACACCTCCTCCTC GAGACCCGGAAAGCTCGGCAGTGGACCATCGAGCTTGAGCCCCCTCAAGCCCAAATCGAT

5 CCCC AATGGCGGCTTGC AGGTT AAGGC AAACGCCAGTGCCCCTCCTAAGATC AATGGTTC

CTCGGTCGGTCTAAAGTCGGGCGGTTTCAAGACTCAGGAAGACTCTCCTTCGGCCCCTCCT CCGCGGACTTTTATCAACCAGTTGCCTGATTGGAGTATGCTTCTTGCTGCAATCACTACTG

TCTTCTTGGCTGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCTAAGAGGCCTGACA

TGCTCGTGGACCCGTTCGGATTGGGAAGTATTGTTCAGGATGGGCTTGTGTTCAGGCAGAA 40 TTTTTCAATTAGGTCCTACGAAATAGGCGCCGATCGAACTGCGTCTATAGAGACGGTGATG

AACCATTTGCAGGAAACAGCTCTCAATCATGTCAAGATTGCTGGGCTTTCTAATGACGGCT TTGGTCGTACTCCTGAGATGTATAAAAGAGACCTTATTTGGGTTGTTGCAAAAATGCAGGT

CATGGTTAACCGCTATCCTACTTGGGGTGACACGGTTGAAGTGAATACTTGGGTTGCCAAG TCAGGGAAAAATGGTATGCGTCGTGACTGGCTCATAAGTGATTGCAATACTGGAGAGATT

CTTACAAGAGCATCAAGCGTGTGGGTCATGATGAATCAAAAGACAAGAAGATTGTCAAAA

ATTCCAGATGAGGTTCGAAATGAGATAGAGCCTCATTTTGTGGACTCTGCTCCCGTCGTTG AAGATGATGATCGGAAACTTCCCAAGCTGGATGAGAACACTGCTGACTCCATCCGCAAGG

GTCTAACTCCGAGGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTACA TCGGATGGATTCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGCTCCCT

0 GACCCTGGAATAC AGGCGGGAATGTGGAAGGGAGAGCGTGCTGGAGTCCCTC ACTGCTGT

CGACCCGTCTGCAGAGGGCTATGCGTCCCGGTTTCAGCACCTTCTGCGGCTTGAGGATGGA GGTGAGATCGTGAAGGCGAGAACTGAGTGGCGACCCAAGAATGCTGGAATCAATGGGGT GGTACCATCCGAGGAGTCCTCACCTGGAGACTTCTTTTAG

2018267601 21 Nov 2018

SEQ ID NO: 33

Cuphea wrightii (Cw) FATB4a coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCGCCGACACCTCCTCCTC CCGCCCCGGCAAGCTGGGCTCCGGCCCCTCCTCCCTGTCCCCCCTGAAGCCCAAGTCCATC

CCCAACGGCGGCCTGCAGGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCC

TCCGTGGGCCTGAAGTCCGGCGGCTTCAAGACCCAGGAGGACTCCCCCTCCGCCCCCCCC

CCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGGCCGCCATCACCACCG

TGTTCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAAGCGCCCCGACA TGCTGGTGGACCCCTTCGGCCTGGGCTCCATCGTGCAGGACGGCCTGGTGTTCCGCCAGAA

CTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATG

AACCACCTGCAGGAGACCGCCCTGAACCACGTGAAGATCGCCGGCCTGTCCAACGACGGC

TTCGGCCGCACCCCCGAGATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAG

GTGATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCC AAGTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAG

ATCCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGACCCGCCGCCTGTCC

AAGATCCCCGACGAGGTGCGCAACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCCGTG

GTGGAGGACGACGACCGCAAGCTGCCCAAGCTGGACGAGAACACCGCCGACTCCATCCG

CAAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGA AGTACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGT

GCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGA

CCGCCGTGGACCCCTCCGCCGAGGGCTACGCCTCCCGCTTCCAGCACCTGCTGCGCCTGGA GGACGGCGGCGAGATCGTGAAGGCCCGCACCGAGTGGCGCCCCAAGAACGCCGGCATCA ACGGCGTGGTGCCCTCCGAGGAGTCCTCCCCCGGCGACTTCTTCTGA

SEQ ID NO: 34

Cuphea wrightii (Cw) FATB4b amino acid sequence

MVATAASSAFFPVPSADTSSSRPGKLGNGPSSLSPLKPKSIPNGGLQVKANASAPPKINGSSVGL KSGSFKTQEDAPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPF GLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSSDGFGRTPAMSK RDLIWWAKMQVMVNRYPAWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSV

0 WVMMNQKTRRLSKIPDEVRNEIEPHFVDSAP WEDDDRKLPKLDENTADSIRKGLTPRWNDL DVNQHVNNVKYIGWILESTPAEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGEGDGSKF QHLLRLEDGGEIVKARTEWRPKNAGINGWPSEESSPGGDFF*

SEQ ID NO: 35

Cuphea wrightii (Cw) FATB4b coding DNA sequence

TTGGTGGCTACCGCTGCAAGTTCTGCATTTTTCCCCGTACCATCCGCCGACACCTCCTCATC GAGACCCGGAAAGCTCGGCAATGGGCCATCGAGCTTGAGCCCCCTCAAGCCGAAATCGAT CCCCAATGGCGGGTTGCAGGTTAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTC CTCGGTCGGTCTGAAGTCGGGCAGTTTCAAGACTCAGGAAGACGCTCCTTCGGCCCCTCCT CCTCGGACTTTTATCAACCAGTTGCCTGATTGGAGTATGCTTCTTGCTGCAATCACTACTGT

CTTCTTGGCTGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCTAAGAGGCCTGACAT

GCTTGTCGACCCGTTCGGATTGGGAAGTATTGTTCAGGATGGGCTTGTTTTCAGGCAGAAT TTCTCGATTAGGTCCTACGAAATAGGCGCTGATCGCACTGCGTCTATAGAGACGGTGATG

AACCATTTGCAGGAAACAGCTCTCAATCATGTTAAGATTGCTGGGCTTTCTAGTGATGGCT TTGGTCGTACTCCTGCGATGTCTAAACGGGACCTCATTTGGGTTGTTGCGAAAATGCAGGT

CATGGTTAACCGCTACCCTGCTTGGGGTGACACGGTTGAAGTGAATACTTGGGTTGCCAA

GTCAGGGAAAAATGGTATGCGTCGTGACTGGCTCATAAGTGATTGCAACACTGGAGAGAT

TCTTACAAGAGCATCAAGCGTGTGGGTCATGATGAATCAAAAGACAAGAAGATTGTCAAA

AATTCCAGATGAGGTTCGAAATGAGATAGAGCCTCATTTTGTGGACTCTGCGCCCGTCGTT GAAGACGATGACCGGAAACTTCCCAAGCTGGATGAGAACACTGCTGACTCCATCCGCAAG

0 GGTCT AACTCCGAGGTGGAATGACTTGGATGTC AATC AGC ACGTC AAC AACGTGAAGTAC

ATTGGGTGGATTCTTGAGAGTACTCCAGCAGAAGTTCTGGAGACCCAGGAATTATGTTCCC

TGACCCTGGAATACAGGCGGGAATGTGGAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTG

TAGATCCGTCTGGAGAGGGCGATGGGTCCAAGTTCCAGCACCTTCTGCGGCTTGAGGATG

2018267601 21 Nov 2018

GAGGTGAGATCGTGAAGGCGAGAACTGAGTGGCGACCAAAGAATGCTGGAATCAATGGG

GTGGTACCATCCGAGGAGTCCTCACCTGGTGGAGACTTCTTTTAA

SEQ ID NO: 36

Cuphea wrightii (Cw) FATB4b coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCGCCGACACCTCCTCCTC

CCGCCCCGGCAAGCTGGGCAACGGCCCCTCCTCCCTGTCCCCCCTGAAGCCCAAGTCCATC CCCAACGGCGGCCTGCAGGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCC

TCCGTGGGCCTGAAGTCCGGCTCCTTCAAGACCCAGGAGGACGCCCCCTCCGCCCCCCCCC

CCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGGCCGCCATCACCACCGT

GTTCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAAGCGCCCCGACAT

GCTGGTGGACCCCTTCGGCCTGGGCTCCATCGTGCAGGACGGCCTGGTGTTCCGCCAGAA

CTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATG

AACCACCTGCAGGAGACCGCCCTGAACCACGTGAAGATCGCCGGCCTGTCCTCCGACGGC

TTCGGCCGCACCCCCGCCATGTCCAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAG

GTGATGGTGAACCGCTACCCCGCCTGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCC

AAGTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAG ATCCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGACCCGCCGCCTGTCC AAGATCCCCGACGAGGTGCGCAACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCCGTG GTGGAGGACGACGACCGCAAGCTGCCCAAGCTGGACGAGAACACCGCCGACTCCATCCG 20 CAAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGA

AGTACATCGGCTGGATCCTGGAGTCCACCCCCGCCGAGGTGCTGGAGACCCAGGAGCTGT

GCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGA

CCGCCGTGGACCCCTCCGGCGAGGGCGACGGCTCCAAGTTCCAGCACCTGCTGCGCCTGG

AGGACGGCGGCGAGATCGTGAAGGCCCGCACCGAGTGGCGCCCCAAGAACGCCGGCATC 25 AACGGCGTGGTGCCCTCCGAGGAGTCCTCCCCCGGCGGCGACTTCTTCTGA

SEQ ID NO: 37

Cuphea wrightii (Cw) FATB5 amino acid sequence

MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPKANGSAVNL

KSGSLETPPRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRIFQDGVFFRQSFSI

RSYEIGVDRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWWTKIQVEVNRYP

TWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNQNTRRLSKFPYEVRQE IAPHFVDSAPVIEDDQKLQKLDVKTGDSIRDGLTPRWNDLDVNQHVNNVKYIGWILKSVPIEV FETQELCGVTLEYRRECGRDSVLESVTAMDPAKEGDRCVYQHLLRLEDGADITIGRTEWRPK NAGANGAMSSGKTSNGNCLIEGRGWQPFRWRLIF*

SEQ ID NO: 38

Cuphea wrightii (Cw) FATB5 coding DNA sequence

ATGGTGGCTGCCGCAGCAAGTTCTGCATTCTTCTCTGTTCCAACCCCGGGAACGCCCCCTA AACCCGGGAAGTTCGGTAACTGGCCATCGAGCTTGAGCGTCCCCTTCAAGCCCGACAATG

GTGGCTTTCATGTCAAGGCAAACGCCAGTGCCCATCCTAAGGCTAATGGTTCTGCGGTAA

ATCTAAAGTCTGGCAGCCTCGAGACTCCTCCTCGGAGTTTCATTAACCAGCTGCCGGACTT

GAGTGTGCTTCTGTCCAAAATCACGACTGTCTTCGGGGCGGCTGAGAAGCAGTGGAAGAG

GCCCGGCATGCTCGTGGAACCGTTTGGGGTTGACAGGATTTTTCAGGATGGTGTTTTTTTC

AGACAGAGTTTTTCTATCAGGTCTTACGAAATAGGCGTTGATCGAACAGCCTCGATAGAG

ACACTGATGAACATCTTCCAGGAAACATCTTTGAATCATTGCAAGAGTATCGGTCTTCTCA

ACGATGGCTTTGGTCGTACTCCTGAGATGTGTAAGAGGGACCTCATTTGGGTGGTTACGAA

AATTCAGGTCGAGGTGAATCGCTATCCTACTTGGGGTGACACTATCGAAGTCAATACTTGG

GTCTCGGAGTCGGGGAAAAACGGTATGGGTCGGGATTGGCTGATAAGTGATTGCCGTACT

GGAGAGATTCTTATAAGAGCAACGAGCGTGTGGGCGATGATGAATCAAAACACGAGAAG

ATTGTCAAAATTTCCATATGAGGTTCGACAGGAGATAGCGCCTCATTTTGTGGACTCTGCT 5 0 CCTGTC ATTGAAGACGATC AAAAGTTGC AGAAGCTTGATGTGAAGAC AGGTGATTCC ATT

CGCGATGGTCTAACTCCGAGATGGAATGACTTGGATGTCAATCAACACGTTAACAATGTG

AAGTACATTGGATGGATTCTCAAGAGTGTTCCAATAGAAGTTTTCGAGACACAGGAGCTA

2018267601 21 Nov 2018

TGCGGCGTCACACTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCAGTG

ACCGCTATGGATCCAGCAAAAGAGGGAGACCGGTGTGTGTACCAGCACCTTCTTCGGCTT

GAGGATGGAGCTGATATCACTATAGGCAGAACCGAGTGGCGGCCGAAGAATGCAGGAGC

CAATGGTGCAATGTCATCAGGAAAGACTTCAAATGGAAACTGTCTCATAGAAGGAAGGGG 5 TTGGCAACCTTTCCGAGTTGTGCGTTTAATTTTCTGA

SEQ ID NO: 39

Cuphea wrightii (Cw) FATB5 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCTCCGTGCCCACCCCCGGCACCCCCCCCA

AGCCCGGCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCGTGCCCTTCAAGCCCGACAACGG 10 CGGCTTCCACGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGAA

CCTGAAGTCCGGCTCCCTGGAGACCCCCCCCCGCTCCTTCATCAACCAGCTGCCCGACCTG TCCGTGCTGCTGTCCAAGATCACCACCGTGTTCGGCGCCGCCGAGAAGCAGTGGAAGCGC

CCCGGCATGCTGGTGGAGCCCTTCGGCGTGGACCGCATCTTCCAGGACGGCGTGTTCTTCC

GCCAGTCCTTCTCCATCCGCTCCTACGAGATCGGCGTGGACCGCACCGCCTCCATCGAGAC 15 CCTGATGAACATCTTCCAGGAGACCTCCCTGAACCACTGCAAGTCCATCGGCCTGCTGAAC

GACGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAG

ATCCAGGTGGAGGTGAACCGCTACCCCACCTGGGGCGACACCATCGAGGTGAACACCTGG

GTGTCCGAGTCCGGCAAGAACGGCATGGGCCGCGACTGGCTGATCTCCGACTGCCGCACC

GGCGAGATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAACACCCGCCGC 20 CTGTCCAAGTTCCCCTACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACTCCGCCC

CCGTGATCGAGGACGACCAGAAGCTGCAGAAGCTGGACGTGAAGACCGGCGACTCCATC

CGCGACGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTG

AAGTACATCGGCTGGATCCTGAAGTCCGTGCCCATCGAGGTGTTCGAGACCCAGGAGCTG

TGCGGCGTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTG 25 ACCGCCATGGACCCCGCCAAGGAGGGCGACCGCTGCGTGTACCAGCACCTGCTGCGCCTG

GAGGACGGCGCCGACATCACCATCGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCGC CAACGGCGCCATGTCCTCCGGCAAGACCTCCAACGGCAACTGCCTGATCGAGGGCCGCGG CTGGCAGCCCTTCCGCGTGGTGCGCCTGATCTTCTGA

SEQ ID NO: 40

Cuphea heterophylla (Cht) FATBla amino acid sequence

MVAAAASSAFFSVPTPGTSTKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASAHPKANGSA

VNLKSGSLETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRPGMLVEPFGVDRI FQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWV VTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNRKT 3 5 RRLSKFPYEVRQEIAPHF VDSAPVIEDDKKLHKLDVKTGDSIRKGLTPRWNDLDVNQHVNNV

KYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVLESVTAMDTAKEGDRSLYQHLLRLEDG

ADITIGRTEWRPKNAGANGAISTGKTSNENSVS*

SEQ ID NO: 41

Cuphea heterophylla (Cht) FATBla coding DNA sequence

ATGGTGGCTGCCGCAGCAAGTTCTGCATTCTTCTCCGTTCCAACCCCGGGAACCTCCACTA

AACCCGGGAACTTCGGCAATTGGCCATCGAGCTTGAGCGTCCCCTTCAAGCCCGAATCAA

ACCACAATGGTGGCTTTCGGGTCAAGGCAAACGCCAGTGCTCATCCTAAGGCTAACGGTT

CTGCAGTAAATCTAAAGTCTGGCAGCCTCGAGACTCAGGAGGACACTTCATCGTCGTCCC

CTCCTCCTCGGACTTTTATTAAGCAGTTGCCCGACTGGGGTATGCTTCTGTCCAAAATCAC 45 GACTGTCTTCGGGGCGGCTGAGAGGCAGTGGAAGAGGCCCGGCATGCTTGTGGAACCGTT

TGGGGTTGACAGGATTTTTCAGGATGGGGTTTTTTTCAGACAGAGTTTTTCGATCAGGTCT

TACGAAATAGGCGCTGATCGAACAGCCTCAATAGAGACGCTGATGAACATCTTCCAGGAA

ACATCTCTGAATCATTGTAAGAGTATCGGTCTTCTCAATGACGGCTTTGGTCGTACTCCTG

AGATGTGTAAGAGGGACCTCATTTGGGTGGTTACGAAAATTCAGGTCGAGGTGAATCGCT 5 0 ATCCT ACTTGGGGTGAT ACTATTGAGGTC AAT ACTTGGGTCTCAGAGTCGGGGAAAAACG

GTATGGGTCGTGATTGGCTGATAAGCGATTGCCGTACCGGAGAAATTCTTATAAGAGCAA

CGAGCGTGTGGGCTATGATGAATCGAAAGACGAGAAGATTGTCAAAATTTCCATATGAGG

2018267601 21 Nov 2018

TTCGACAGGAGATAGCGCCTCATTTTGTGGACTCTGCTCCTGTCATTGAAGACGATAAAAA

ATTGCACAAGCTTGATGTTAAGACGGGTGATTCCATTCGCAAGGGTCTAACTCCAAGGTG

GAATGACTTGGATGTCAATCAGCACGTTAACAATGTGAAGTACATTGGGTGGATTCTCAA

GAGTGTTCCAGCAGAAGTTTTCGAGACCCAGGAGCTATGCGGAGTCACCCTTGAGTACAG

GCGGGAATGTGGAAGGGACAGTGTGCTGGAGTCCGTGACCGCTATGGATACCGCAAAAG

AGGGAGACCGGTCTCTGTACCAGCACCTTCTTCGGCTTGAGGATGGGGCTGATATCACCAT AGGCAGAACCGAGTGGCGGCCGAAGAATGCAGGAGCCAATGGGGCAATATCAACAGGAA AGACTTCAAATGAAAACTCTGTCTCTTAG

SEQ ID NO: 42

Cuphea heterophylla (Cht) FATBla coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCTCCGTGCCCACCCCCGGCACCTCCACCA

AGCCCGGCAACTTCGGCAACTGGCCCTCCTCCCTGTCCGTGCCCTTCAAGCCCGAGTCCAA

CCACAACGGCGGCTTCCGCGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC 15 CGCCGTGAACCTGAAGTCCGGCTCCCTGGAGACCCAGGAGGACACCTCCTCCTCCTCCCCC

CCCCCCCGCACCTTCATCAAGCAGCTGCCCGACTGGGGCATGCTGCTGTCCAAGATCACCA

CCGTGTTCGGCGCCGCCGAGCGCCAGTGGAAGCGCCCCGGCATGCTGGTGGAGCCCTTCG

GCGTGGACCGCATCTTCCAGGACGGCGTGTTCTTCCGCCAGTCCTTCTCCATCCGCTCCTA

CGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGAACATCTTCCAGGAGAC 20 CTCCCTGAACCACTGCAAGTCCATCGGCCTGCTGAACGACGGCTTCGGCCGCACCCCCGA

GATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGATCCAGGTGGAGGTGAACCGCTA

CCCCACCTGGGGCGACACCATCGAGGTGAACACCTGGGTGTCCGAGTCCGGCAAGAACGG

CATGGGCCGCGACTGGCTGATCTCCGACTGCCGCACCGGCGAGATCCTGATCCGCGCCAC

CTCCGTGTGGGCCATGATGAACCGCAAGACCCGCCGCCTGTCCAAGTTCCCCTACGAGGT

GCGCCAGGAGATCGCCCCCCACTTCGTGGACTCCGCCCCCGTGATCGAGGACGACAAGAA

GCTGCACAAGCTGGACGTGAAGACCGGCGACTCCATCCGCAAGGGCCTGACCCCCCGCTG

GAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGATCCTGAA

GTCCGTGCCCGCCGAGGTGTTCGAGACCCAGGAGCTGTGCGGCGTGACCCTGGAGTACCG

CCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGGACACCGCCAAGGA 30 GGGCGACCGCTCCCTGTACCAGCACCTGCTGCGCCTGGAGGACGGCGCCGACATCACCAT

CGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCGCCAACGGCGCCATCTCCACCGGCAA GACCTCCAACGAGAACTCCGTGTCCTGA

SEQ ID NO: 43

Cuphea heterophylla (Cht) FATBlb (P16S, T20P, G94S, G105W, S293F, L305F variant) amino acid sequence

MVAAAASSAFFSVPTSGTSPKPGNFGNWPSSLSVPFKPESSHNGGFQVKANASAHPKANGSAV

NLKSGSLETQEDTSSSSPPPRTFIKQLPDWSMLLSKITTVFWAAERQWKRPGMLVEPFGVDRIF

QDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWW

TKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNRKTRR

LSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGDFIRKGLTPRWNDFDVNQHVNNVKYI

GWILKSVPAEVFETQELCGVTLEYRRECGRDSVLESVTAMDTAKEGDRSLYQHLLRLEDGADI TIGRTEWRPKNAGANGAISTGKTSNENSVS*

SEQ ID NO: 44

Cuphea heterophylla (Cht) FATBlb(P16S, T20P, G94S, G105W, S293F, L305F variant) coding DNA sequence

ATGGTGGCTGCCGCAGCAAGTTCTGCATTCTTCTCCGTTCCAACCTCGGGAACCTCCCCTA

AACCCGGGAACTTCGGCAATTGGCCATCGAGCTTGAGCGTCCCCTTCAAGCCCGAATCAA

GCCACAATGGTGGCTTTCAGGTCAAGGCAAACGCCAGTGCCCATCCTAAGGCTAACGGTT

CTGCAGTAAATCTAAAGTCTGGCAGCCTCGAGACTCAGGAGGACACTTCATCGTCGTCCC

0 CTCCTCCTCGGACTTTTATTAAGC AGTTGCCCGACTGGAGT ATGCTTCTGTCCAAAATC AC

GACTGTCTTCTGGGCGGCTGAGAGGCAGTGGAAGAGGCCCGGCATGCTTGTGGAACCGTT

TGGGGTTGACAGGATTTTTCAGGATGGGGTTTTTTTCAGACAGAGTTTTTCGATCAGGTCT

2018267601 21 Nov 2018

TACGAAATAGGCGCTGATCGAACAGCCTCAATAGAGACGCTGATGAACATCTTCCAGGAA

ACATCTCTGAATCATTGTAAGAGTATCGGTCTTCTCAATGACGGCTTTGGTCGTACTCCTG

AGATGTGTAAGAGGGACCTCATTTGGGTGGTTACGAAAATTCAGGTCGAGGTGAATCGCT

ATCCTACTTGGGGTGATACTATTGAGGTCAATACTTGGGTCTCAGAGTCGGGGAAAAACG

GTATGGGTCGTGATTGGCTGATAAGCGATTGCCGTACCGGAGAAATTCTTATAAGAGCAA

CGAGCGTGTGGGCTATGATGAATCGAAAGACGAGAAGATTGTCAAAATTTCCATATGAGG TTCGACAGGAGATAGCGCCTCATTTTGTGGACTCTGCTCCTGTCATTGAAGACGATAAAAA

ATTGCACAAGCTTGATGTTAAGACGGGTGATTTCATTCGCAAGGGTCTAACTCCAAGGTG

GAATGACTTTGATGTCAATCAGCACGTTAACAATGTGAAGTACATTGGGTGGATTCTCAA

GAGTGTTCCAGCAGAAGTTTTCGAGACCCAGGAGCTATGCGGAGTCACCCTTGAGTATAG

GCGGGAATGTGGAAGGGACAGTGTGCTGGAGTCCGTGACCGCTATGGATACCGCAAAAG AGGGAGACCGGTCTCTGTACCAGCACCTTCTTCGGCTTGAGGATGGGGCTGATATCACCAT

AGGCAGAACCGAGTGGCGGCCGAAGAATGCAGGAGCCAATGGGGCAATATCAACAGGAA AGACTTCAAATGAAAACTCTGTCTCTTAG

SEQ ID NO: 45

Cuphea heterophylla (Cht) FATBlb (P16S, T20P, G94S, G105W, S293F, L305F variant) coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCTCCGTGCCCACCTCCGGCACCTCCCCCA

AGCCCGGCAACTTCGGCAACTGGCCCTCCTCCCTGTCCGTGCCCTTCAAGCCCGAGTCCTC 20 CCACAACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC

CGCCGTGAACCTGAAGTCCGGCTCCCTGGAGACCCAGGAGGACACCTCCTCCTCCTCCCCC

CCCCCCCGCACCTTCATCAAGCAGCTGCCCGACTGGTCCATGCTGCTGTCCAAGATCACCA

CCGTGTTCTGGGCCGCCGAGCGCCAGTGGAAGCGCCCCGGCATGCTGGTGGAGCCCTTCG

GCGTGGACCGCATCTTCCAGGACGGCGTGTTCTTCCGCCAGTCCTTCTCCATCCGCTCCTA

CGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGAACATCTTCCAGGAGAC

CTCCCTGAACCACTGCAAGTCCATCGGCCTGCTGAACGACGGCTTCGGCCGCACCCCCGA

GATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGATCCAGGTGGAGGTGAACCGCTA

CCCCACCTGGGGCGACACCATCGAGGTGAACACCTGGGTGTCCGAGTCCGGCAAGAACGG

CATGGGCCGCGACTGGCTGATCTCCGACTGCCGCACCGGCGAGATCCTGATCCGCGCCAC

0 CTCCGTGTGGGCC ATGATGAACCGCAAGACCCGCCGCCTGTCCAAGTTCCCCT ACGAGGT

GCGCCAGGAGATCGCCCCCCACTTCGTGGACTCCGCCCCCGTGATCGAGGACGACAAGAA

GCTGCACAAGCTGGACGTGAAGACCGGCGACTTCATCCGCAAGGGCCTGACCCCCCGCTG

GAACGACTTCGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGATCCTGAA

GTCCGTGCCCGCCGAGGTGTTCGAGACCCAGGAGCTGTGCGGCGTGACCCTGGAGTACCG

5 CCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCC ATGGAC ACCGCCAAGGA

GGGCGACCGCTCCCTGTACCAGCACCTGCTGCGCCTGGAGGACGGCGCCGACATCACCAT CGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCGCCAACGGCGCCATCTCCACCGGCAA GACCTCCAACGAGAACTCCGTGTCCTGA

SEQ ID NO: 46

Cuphea heterophylla (Cht) FATB2b amino acid sequence

MWAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSA

VSLKSGSLNTQEGTSSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQLTMLDRKSKKPDMHVD WFGLEIIVQDGLVFRESFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTPEM

CKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWA

MMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPEWNDLDV

NQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGDRSQYQ

HLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS*

SEQ ID NO: 47

Cuphea heterophylla (Cht) FATB2b coding DNA sequence

0 ATGGTGGTGGCTGCTGC AGC AAGCTCTGC ATTCTTCCCTGTTCCGGC ATCTGGAACCTCCC

CTAAACCCGGGAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGT

CAAACCCCAGTGGTGGATTTCAGGTTAAGGCAAATGCCAGTGCTCATCCTAAGGCTAACG

2018267601 21 Nov 2018

GTTCCGCAGTAAGTCTAAAGTCTGGCAGCCTCAACACTCAGGAGGGCACTTCGTCGTCCCC

TCCTCCTCGGACTTTCCTTAACCAGTTGCCTGATTGGAGTAGGCTTCGGACTGCAATCACG

ACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAAGTCTAAGAAGCCT

GACATGCACGTGGACTGGTTTGGGTTGGAGATTATTGTTCAGGATGGGCTCGTGTTCAGAG

AGAGTTTTTCGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAAACGTT

GATGAACCATTTGCAGGACACATCTTTGAACCATTGTAAGAGTGTGGGTCTTCTCAATGAC

GGCTTTGGTCGTACCCCGGAGATGTGTAAAAGGGACCTCATTTGGGTGCTTACAAAAATG

CAGATCATGGTGAATCGCTATCCAACTTGGGGCGATACTGTCGAGATCAATAGCTGGTTCT

CCCAGTCCGGGAAAATCGGTATGGGTCGCAATTGGCTAATAAGTGATTGCAACACAGGAG

AAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCAAAAGACGAGAAGATTCT

CAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCCCCTCCTGT

CATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTTGC

AAGGGTCTAACACCGGAGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAG

TACATTGGGTGGATTCTCGAGAGTATGCCAAAAGAAGTTTTGGACACCCAGGAGCTATGC

TCTCTCACCCTTGAATATAGGCGGGAATGCGGAAGGGATAGTGTGCTGGAGTCTGTGACC

GCTATGGATCCCTCAAAAGTTGGAGACCGATCTCAGTACCAGCACCTTCTGCGGCTTGAA GATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACCAAAGAATGCAGGAACCAA CGGGGCTATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG

SEQ ID NO: 48

Cuphea heterophylla (Cht) FATB2b coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCTCCGGCACCTCCC

CCAAGCCCGGCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTC

CAACCCCTCCGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGG

CTCCGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCCAGGAGGGCACCTCCTCCTCCCCC

CCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGTCCCGCCTGCGCACCGCCATCACCA

CCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAAGTCCAAGAAGCCCG

ACATGCACGTGGACTGGTTCGGCCTGGAGATCATCGTGCAGGACGGCCTGGTGTTCCGCG

AGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCT

0 GATGAACCACCTGCAGGACACCTCCCTGAACC ACTGC AAGTCCGTGGGCCTGCTGAACGA

CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGAT

GCAGATCATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACTCCTGGTT

CTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACTGGCTGATCTCCGACTGCAACACCGG

CGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCAGAAGACCCGCCGCTT

5 CTCC AAGCTGCCC AACGAGGTGCGCC AGGAGATCGCCCCCC ACTTCGTGGACGCCCCCCC

CGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCAT

CTGCAAGGGCCTGACCCCCGAGTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGT

GAAGTACATCGGCTGGATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCT

GTGCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGT

GACCGCCATGGACCCCTCCAAGGTGGGCGACCGCTCCCAGTACCAGCACCTGCTGCGCCT

GGAGGACGGCACCGACATCATGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA

CCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA

SEQ ID NO: 49

Cuphea heterophylla (Cht) FATB2a (S17P, P21S, T28N, L30P, S33L, G76D, S78P, G137W variant) 45 amino acid sequence

MWAAAASSAFFPVPAPGTTSKPGKFGNWPSSLSPSFKPKSNPNGGFQVKANASAHPKANGS

AVSLKSGSLNTKEDTPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQLTMLDRKSKKPDMHV DWFGLEIIVQDWLVFRESFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTPE

MCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIW 50 AMMNQKTRRFSKLPNEVRQEIAPHFVDAPPLIEDNDRKLHKFDVKTGDSICKGLTPEWNDLD

VNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGDRSQY

QHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS*

2018267601 21 Nov 2018

SEQ ID NO: 50

Cuphea heterophylla (Cht) FATB2a (S17P, P21S, T28N, L30P, S33L, G76D, S78P, G137W variant) coding DNA sequence

ATGGTGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGTTCCAGCACCTGGAACCACGT

CTAAACCCGGGAAGTTCGGCAATTGGCCATCGAGCTTGAGCCCTTCCTTCAAGCCCAAGTC

AAACCCCAATGGTGGATTTCAGGTTAAGGCAAATGCCAGCGCTCATCCTAAGGCTAACGG

GTCTGCAGTAAGTCTAAAGTCTGGCAGCCTCAACACTAAGGAGGACACTCCGTCGTCCCC

TCCTCCTCGGACTTTCCTTAACCAGTTGCCTGATTGGAGTAGGCTTCGGACTGCAATCACG

ACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAAGTCTAAGAAGCCT

GACATGCACGTGGACTGGTTTGGGTTGGAGATTATTGTTCAGGATTGGCTCGTGTTCAGAG

AGAGTTTTTCGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAAACGTT

GATGAACCATTTGCAGGACACATCTTTGAACCATTGTAAGAGTGTGGGTCTTCTCAATGAC

GGCTTTGGTCGTACCCCGGAGATGTGTAAAAGGGACCTCATTTGGGTGCTTACAAAAATG

CAGATCATGGTGAATCGCTATCCAACTTGGGGCGATACTGTCGAGATCAATAGCTGGTTCT 15 CCCAGTCCGGGAAAATCGGTATGGGTCGCAATTGGCTAATAAGTGATTGCAACACAGGAG

AAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCAAAAGACGAGAAGATTCT

CAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCTCCTCATTTTGTGGACGCCCCTCCTCT

CATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTTGC

AAGGGTCTAACACCGGAGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAG 20 TACATTGGGTGGATTCTCGAGAGTATGCCAAAAGAAGTTTTGGACACCCAGGAGCTATGC

TCTCTCACCCTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCTGTGACC

GCTATGGATCCCTCAAAAGTTGGAGACCGATCTCAGTACCAGCACCTTCTGCGGCTTGAA

GATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACCAAAGAATGCAGGAACCAA

CGGGGCGATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG

SEQ ID NO: 51

Cuphea heterophylla (Cht) FATB2a (S17P, P21S, T28N, L30P, S33L, G76D, S78P, G137W variant) coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCACCT

CCAAGCCCGGCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGCCCAAGTC 3 0 C AACCCC AACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCC AAGGCC AACGG

CTCCGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCAAGGAGGACACCCCCTCCTCCCCC

CCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGTCCCGCCTGCGCACCGCCATCACCA

CCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAAGTCCAAGAAGCCCG

ACATGCACGTGGACTGGTTCGGCCTGGAGATCATCGTGCAGGACTGGCTGGTGTTCCGCG

AGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCT

GATGAACCACCTGCAGGACACCTCCCTGAACCACTGCAAGTCCGTGGGCCTGCTGAACGA

CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGAT

GCAGATCATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACTCCTGGTT

CTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACTGGCTGATCTCCGACTGCAACACCGG 40 CGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCAGAAGACCCGCCGCTT

CTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCCCCCCC

CCTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCAT

CTGCAAGGGCCTGACCCCCGAGTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGT

GAAGTACATCGGCTGGATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCT 45 GTGCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGT

GACCGCCATGGACCCCTCCAAGGTGGGCGACCGCTCCCAGTACCAGCACCTGCTGCGCCT GGAGGACGGCACCGACATCATGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA CCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA

SEQ ID NO: 52

Cuphea heterophylla (Cht) FATB2c (G76D, S78P variant) amino acid sequence

MWAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSA

VSLKSGSLNTKEDTPSSPPPRTFLNQLPDWNRLRTAITTVFVAAEKQLTMLDRKSKKPDMHVD

WFGLEIIVQDGLVFRESFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTPEM

2018267601 21 Nov 2018

CKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWA MMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPEWNDLDV NQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGDRSQYQ HLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS*

SEQ ID NO: 53

Cuphea heterophylla (Cht) FATB2c (G76D, S78P variant) coding DNA sequence

ATGGTGGTGGCTGCTGCAGCAAGCTCTGCATTCTTCCCTGTTCCGGCATCTGGAACCTCCC CTAAACCCGGGAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGT

CAAACCCCAGTGGTGGATTTCAGGTTAAGGCAAATGCCAGTGCTCATCCTAAGGCTAACG

GTTCCGCAGTAAGTCTAAAGTCTGGCAGCCTCAACACTAAGGAGGACACTCCGTCGTCCC CTCCTCCTCGGACTTTCCTTAACCAGTTGCCTGATTGGAATAGGCTTCGGACTGCAATCAC GACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAAGTCTAAGAAGCC TGACATGCACGTGGACTGGTTTGGGTTGGAGATTATTGTTCAGGATGGGCTCGTGTTCAGA GAGAGTTTTTCGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAAACG

TTGATGAACCATTTGCAGGACACATCTTTGAACCATTGTAAGAGTGTGGGTCTTCTCAATG ACGGCTTTGGTCGTACCCCGGAGATGTGTAAAAGGGACCTCATTTGGGTGCTTACAAAAA TGCAGATCATGGTGAATCGCTATCCAACTTGGGGCGATACTGTCGAGATCAATAGCTGGTT CTCCCAGTCCGGGAAAATCGGTATGGGTCGCAATTGGCTAATAAGTGATTGCAACACAGG AGAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCAAAAGACGAGAAGATT

CTCAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCCCCTCCT

GTCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTT GCAAGGGTCTAACACCGGAGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGA

AGTACATTGGGTGGATTCTCGAGAGTATGCCAAAAGAAGTTTTGGACACCCAGGAGCTAT GCTCTCTCACCCTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCTGTGA

CCGCTATGGATCCCTCAAAAGTTGGGGACCGATCTCAGTACCAGCACCTTCTGCGGCTTGA AGATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACCAAAGAATGCAGGAACCA ACGGGGCTATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG

SEQ ID NO: 54

Cuphea heterophylla (Cht) FATB2c (G76D, S78P variant) coding DNA sequence codon optimized for

Prototheca moriformis

ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCTCCGGCACCTCCC CCAAGCCCGGCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTC

CAACCCCTCCGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGG CTCCGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCAAGGAGGACACCCCCTCCTCCCCC

5 CCCCCCCGC ACCTTCCTGAACCAGCTGCCCGACTGGAACCGCCTGCGCACCGCC ATC ACC A

CCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAAGTCCAAGAAGCCCG ACATGCACGTGGACTGGTTCGGCCTGGAGATCATCGTGCAGGACGGCCTGGTGTTCCGCG AGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCT GATGAACCACCTGCAGGACACCTCCCTGAACCACTGCAAGTCCGTGGGCCTGCTGAACGA

CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGAT

GCAGATCATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACTCCTGGTT CTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACTGGCTGATCTCCGACTGCAACACCGG

CGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCAGAAGACCCGCCGCTT CTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCCCCCCC

CGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCAT

CTGCAAGGGCCTGACCCCCGAGTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGT GAAGTACATCGGCTGGATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCT

GTGCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGT GACCGCCATGGACCCCTCCAAGGTGGGCGACCGCTCCCAGTACCAGCACCTGCTGCGCCT

0 GGAGGACGGC ACCGACATCATGAAGGGCCGC ACCGAGTGGCGCCCC AAGAACGCCGGCA

CCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA

2018267601 21 Nov 2018

SEQ ID NO: 55

Cuphea heterophylla (Cht) FATB2d (S21P, T28N, L30P, S33L, G76D, R97L, H124L, W127L, I132S,

K258N, C303R, E309G, K334T, T386A variant) amino acid sequence

MWAAAASSAFFPVPAPGTTSKPGKFGNWPSSLSPSFKPKSNPNGGFQVKANASAHPKANGS

AVSLKSGSLNTQEDTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRPDMLV

DLFGLESIVQDGLVFRESYSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTPE

MCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIW

AMMNQNTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSIRKGLTPGWNDLD

VNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRRECGRESVLESVTAMNPSKVGDRSQYQ 10 HLLRLEDGADIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS*

SEQ ID NO: 56

Cuphea heterophylla (Cht) FATB2d (S21P, T28N, L30P, S33L, G76D, R97L, H124L, W127L, I132S,

K258N, C303R, E309G, K334T, T386A variant) coding DNA sequence

ATGGTGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGTTCCAGCACCTGGAACCACGT

CTAAACCCGGGAAGTTCGGCAATTGGCCATCGAGCTTGAGCCCTTCCTTCAAGCCCAAGTC

AAACCCCAATGGTGGATTTCAGGTTAAGGCAAATGCCAGTGCTCATCCTAAGGCTAACGG

TTCTGCGGTAAGTCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCCT

CCTCCTCGGACATTCCTTAACCAGTTGCCTGATTGGAGTAGGCTTCTGACTGCAATCTCGA

CCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAAATCTAAGAGGCCTG

ACATGCTCGTGGACTTGTTTGGGTTGGAGAGTATTGTTCAGGATGGGCTCGTGTTCAGAGA

GAGTTATTCGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAAACGTT

GATGAACCATTTGCAGGACACATCTTTGAACCATTGTAAGAGTGTGGGTCTTCTCAATGAC

GGCTTTGGTCGTACCCCGGAGATGTGTAAAAGGGACCTCATTTGGGTGCTTACAAAAATG

CAGATCATGGTGAATCGCTATCCAACTTGGGGCGATACTGTCGAGATCAATAGCTGGTTCT 25 CCCAGTCCGGGAAAATCGGTATGGGTCGCAATTGGCTAATAAGTGATTGCAACACAGGAG

AAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCAAAATACGAGAAGATTCT

CAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTTGACGCTCCTCCTGT

CATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTCG

CAAGGGTCTAACTCCGGGGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAA 3 0 GT ACATTGGGTGGATTCTCGAGAGTATGCC AACAGAAGTTTTGGAGACCC AGGAGCTATG

CTCTCTCACCCTTGAATATAGGCGGGAATGCGGAAGGGAAAGTGTGCTGGAGTCCGTGAC

CGCTATGAATCCCTCAAAAGTTGGAGACCGGTCTCAGTACCAGCACCTTCTACGGCTTGAG

GATGGGGCTGATATCATGAAGGGCAGAACTGAGTGGCGACCAAAGAATGCAGGAACCAA

CGGGGCGATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG

SEQ ID NO: 57

Cuphea heterophylla (Cht) FATB2d (S21P, T28N, L30P, S33L, G76D, R97L, H124L, W127L, I132S,

K258N, C303R, E309G, K334T, T386A variant) coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCACCT 40 CCAAGCCCGGCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGCCCAAGTC

CAACCCCAACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGG

CTCCGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCC

CCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGTCCCGCCTGCTGACCGCCATCTCCA

CCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAAGTCCAAGCGCCCCG 45 ACATGCTGGTGGACCTGTTCGGCCTGGAGTCCATCGTGCAGGACGGCCTGGTGTTCCGCG

AGTCCTACTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCT

GATGAACCACCTGCAGGACACCTCCCTGAACCACTGCAAGTCCGTGGGCCTGCTGAACGA

CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGAT

GCAGATCATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACTCCTGGTT 5 0 CTCCCAGTCCGGC AAGATCGGC ATGGGCCGCAACTGGCTGATCTCCGACTGCAACACCGG

CGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCAGAACACCCGCCGCTT

CTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCCCCCCC

CGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCAT

2018267601 21 Nov 2018

CCGCAAGGGCCTGACCCCCGGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGT GAAGTACATCGGCTGGATCCTGGAGTCCATGCCCACCGAGGTGCTGGAGACCCAGGAGCT GTGCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCGT GACCGCCATGAACCCCTCCAAGGTGGGCGACCGCTCCCAGTACCAGCACCTGCTGCGCCT GGAGGACGGCGCCGACATCATGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA CCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA

SEQ ID NO: 58

Cuphea heterophylla (Cht) FATB2e (G76D, R97L, H124L, I132S, G152S, H165L, T211N, K258N, C303R, E309G, K334T, T386A variant) amino acid sequence

MWAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSA VSLKSGSLNTQEDTSSSPPPQTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRPDMLVD WFGLESIVQDGLVFRESYSIRSYEISADRTASIETVMNLLQETSLNHCKSMGILNDGFGRTPEM CKRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWA MMNQNTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSIRKGLTPGWNDLDV NQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRRECGRDSVLESVTAMNPSKVGDRSQYQ HLLRLEDGADIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS*

SEQ ID NO: 59

Cuphea heterophylla (Cht) FATB2e (G76D, R97L, H124L, I132S, G152S, H165L, T211N, K258N, C303R, E309G, K334T, T386A variant) coding DNA sequence

ATGGTGGTGGCTGCTGCAGCAAGCTCTGCATTCTTCCCTGTTCCGGCATCTGGAACCTCCC CTAAACCCGGGAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGT CAAACCCCAGTGGTGGATTTCAGGTTAAGGCAAATGCCAGTGCTCATCCTAAGGCTAACG GTTCTGCAGTAAGTCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCC TCCTCCTCAGACATTCCTTAACCAGTTGCCTGATTGGAGTAGGCTTCTGACAGCAATCTCG ACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAAATCTAAAAGGCCT GACATGCTCGTGGACTGGTTTGGGTTGGAGAGTATTGTTCAGGATGGGCTCGTGTTCAGAG AGAGTTATTCGATCAGGTCTTACGAAATAAGCGCTGATCGAACAGCCTCTATAGAGACGG TGATGAACCTCTTGCAGGAAACATCTCTCAATCATTGTAAGAGTATGGGTATTCTCAATGA CGGCTTTGGTCGTACCCCGGAGATGTGCAAAAGGGACCTCATTTGGGTGCTTACAAAAAT GCAGATCTTGGTGAATCGCTATCCAAATTGGGGTGATACTGTCGAGATCAATAGCTGGTTC TCCCAGTCCGGGAAAATCGGTATGGGTCGCAATTGGCTAATAAGTGATTGCAACACAGGA GAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCAAAATACGAGAAGATTC TCAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTTGACGCTCCTCCTG TCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTCG CAAGGGTCTAACTCCGGGGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAA GTACATTGGGTGGATTCTCGAGAGTATGCCAACAGAAGTTTTGGAGACCCAGGAGCTATG CTCTCTCACCCTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCCGTGAC CGCTATGAATCCCTCAAAAGTTGGAGACCGGTCTCAGTACCAGCACCTTCTACGGCTTGAG GATGGGGCTGATATCATGAAGGGCAGAACTGAGTGGCGACCAAAGAATGCAGGAACCAA CGGGGCGATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG

SEQ ID NO: 60

Cuphea heterophylla (Cht) FATB2e (G76D, R97L, H124L, I132S, G152S, H165L, T211N, K258N,

C303R, E309G, K334T, T386A variant) coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCTCCGGCACCTCCC CCAAGCCCGGCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTC CAACCCCTCCGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGG CTCCGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCC CCCCCCCAGACCTTCCTGAACCAGCTGCCCGACTGGTCCCGCCTGCTGACCGCCATCTCCA CCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAAGTCCAAGCGCCCCG ACATGCTGGTGGACTGGTTCGGCCTGGAGTCCATCGTGCAGGACGGCCTGGTGTTCCGCG AGTCCTACTCCATCCGCTCCTACGAGATCTCCGCCGACCGCACCGCCTCCATCGAGACCGT

2018267601 21 Nov 2018

GATGAACCTGCTGCAGGAGACCTCCCTGAACCACTGCAAGTCCATGGGCATCCTGAACGA

CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGAT GCAGATCCTGGTGAACCGCTACCCCAACTGGGGCGACACCGTGGAGATCAACTCCTGGTT

CTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACTGGCTGATCTCCGACTGCAACACCGG

CGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCAGAACACCCGCCGCTT CTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCCCCCCC CGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCAT CCGCAAGGGCCTGACCCCCGGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGT GAAGTACATCGGCTGGATCCTGGAGTCCATGCCCACCGAGGTGCTGGAGACCCAGGAGCT

GTGCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGT

GACCGCCATGAACCCCTCCAAGGTGGGCGACCGCTCCCAGTACCAGCACCTGCTGCGCCT GGAGGACGGCGCCGACATCATGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA

CCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA

SEQ ID NO: 61

Cuphea heterophylla (Cht) FATB2f (R97L, H124L, I132S, G152S, H165L, T211N variant) amino acid sequence

MWAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSA VSLKSGSLNTQEGTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRPDMLVD WFGLESIVQDGLVFRESYSIRSYEISADRTASIETVMNLLQETSLNHCKSMGILNDGFGRTPEM

CKRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWA MMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPEWNDLDV NQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGDRSQYQ HLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS*

SEQ ID NO: 62

Cuphea heterophylla (Cht) FATB2f (R97L, H124L, I132S, G152S, H165L, T21 IN variant) coding

DNA sequence

ATGGTGGTGGCTGCTGCAGCAAGCTCTGCATTCTTCCCTGTTCCGGCATCTGGAACCTCCC CTAAACCCGGGAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGT

CAAACCCCAGTGGTGGATTTCAGGTTAAAGCAAATGCCAGTGCTCATCCTAAGGCTAACG

0 GTTCCGCAGTAAGTCTAAAGTCTGGCAGCCTCAAC ACTC AGGAGGGC ACTTCGTCGTCCCC

TCCTCCTCGGACATTCCTTAACCAGTTGCCTGATTGGAGTAGGCTTCTGACTGCAATCTCG ACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAAATCTAAGAGGCCT

GACATGCTCGTGGACTGGTTTGGGTTGGAGAGTATTGTTCAGGATGGGCTCGTGTTCAGAG AGAGTTATTCGATCAGGTCTTACGAAATAAGCGCTGATCGAACAGCCTCTATAGAGACGG

5 TGATGAACCTCTTGC AGGAAACATCTCTC AATC ATTGTAAGAGTATGGGT ATTCTC AATGA

CGGCTTTGGTCGTACCCCGGAGATGTGCAAAAGGGACCTCATTTGGGTGCTTACAAAAAT GCAGATCTTGGTGAATCGCTATCCAAATTGGGGTGATACTGTCGAGATCAATAGCTGGTTC

TCCCAGTCCGGGAAAATCGGTATGGGTCGCAATTGGCTAATAAGTGATTGCAACACAGGA GAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCAAAAGACGAGAAGATTC

TCAAAACTTCCAAATGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCCCCTCCTG

TCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTTG CAAGGGTCTAACACCGGAGTGGAACGACTTGGATGTCAATCAGCACGTAAGCAACGTGAA

GTACATTGGGTGGATTCTCGAGAGTATGCCAAAAGAAGTTTTGGACACCCAGGAGCTATG CTCTCTCACCCTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCTGTGAC

CGCTATGGATCCCTCAAAAGTTGGAGACCGATCTCAGTACCAGCACCTTCTGCGGCTTGAA GATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACCAAAGAATGCAGGAACCAA CGGGGCGATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG

SEQ ID NO: 63

Cuphea heterophylla (Cht) FATB2f (R97L, H124L, I132S, G152S, H165L, T21 IN variant) coding

DNA sequence codon optimized for Prototheca moriformis

ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCTCCGGCACCTCCC CCAAGCCCGGCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTC

2018267601 21 Nov 2018

CAACCCCTCCGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGG

CTCCGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCCAGGAGGGCACCTCCTCCTCCCCC

CCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGTCCCGCCTGCTGACCGCCATCTCCA

CCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAAGTCCAAGCGCCCCG

ACATGCTGGTGGACTGGTTCGGCCTGGAGTCCATCGTGCAGGACGGCCTGGTGTTCCGCG

AGTCCTACTCCATCCGCTCCTACGAGATCTCCGCCGACCGCACCGCCTCCATCGAGACCGT

GATGAACCTGCTGCAGGAGACCTCCCTGAACCACTGCAAGTCCATGGGCATCCTGAACGA

CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGAT

GCAGATCCTGGTGAACCGCTACCCCAACTGGGGCGACACCGTGGAGATCAACTCCTGGTT

CTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACTGGCTGATCTCCGACTGCAACACCGG

CGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCAGAAGACCCGCCGCTT

CTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCCCCCCC

CGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCAT

CTGCAAGGGCCTGACCCCCGAGTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGT 15 GAAGTACATCGGCTGGATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCT

GTGCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGT

GACCGCCATGGACCCCTCCAAGGTGGGCGACCGCTCCCAGTACCAGCACCTGCTGCGCCT

GGAGGACGGCACCGACATCATGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA

CCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA

SEQ ID NO: 64

Cuphea heterophylla (Cht) FATB2g (A6T, A16V, S17P, G76D, R97L, H124L, I132S, S1431, G152S,

A157T, H165L, T21 IN, G414A variant) amino acid sequence

MWAATASSAFFPVPVPGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSA

VSLKSGSLNTQEDTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRPDMLVD

WFGLESIVQDGLVFREIYSIRSYEISADRTTSIETVMNLLQETSLNHCKSMGILNDGFGRTPEMC

KRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWAM MNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPEWNDLDVN QHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGDRSQYQH LLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNANSVS*

SEQ ID NO: 65

Cuphea heterophylla (Cht) FATB2g (A6T, A16V, S17P, G76D, R97L, H124L, I132S, S143I, G152S,

A157T, H165L, T21 IN, G414A variant) coding DNA sequence

ATGGTGGTGGCTGCTACAGCAAGTTCTGCATTCTTCCCTGTTCCTGTACCTGGAACCTCCC

CTAAACCCGGAAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGT

5 C AAACCCCAGTGGTGGATTTCAGGTTAAGGCAAATGCCAGTGCTC ATCCTAAGGCT AACG

GTTCTGCAGTAAGTCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCC

TCCTCCTCGGACATTCCTTAACCAGTTGCCTGATTGGAGTAGGCTTCTGACTGCAATCTCG

ACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAAATCTAAGAGGCCT

GACATGCTCGTGGACTGGTTTGGGTTGGAGAGTATTGTTCAGGATGGGCTCGTGTTCAGAG 40 AGATTTATTCGATCAGGTCTTACGAAATAAGCGCTGATCGAACAACCTCTATAGAGACGG

TGATGAACCTCTTGCAGGAAACATCTCTCAATCATTGTAAGAGTATGGGTATTCTCAATGA CGGCTTTGGTCGTACCCCGGAGATGTGCAAAAGGGACCTCATTTGGGTGCTTACAAAAAT

GCAGATCTTGGTGAATCGCTATCCAAATTGGGGTGATACTGTCGAGATCAATAGCTGGTTC

TCCCAGTCCGGGAAAATCGGTATGGGTCGCAATTGGCTAATAAGTGATTGCAACACAGGA 45 GAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCAAAAGACGAGAAGATTC

TCAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCCCCTCCTG

TCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTTG

CAAGGGTCTAACACCGGAGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAA

GTACATTGGGTGGATTCTCGAGAGTATGCCAAAAGAAGTTTTGGACACCCAGGAGCTATG 5 0 CTCTCTC ACCCTTGAATAT AGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCTGTGAC

CGCTATGGATCCCTCAAAAGTTGGAGACCGATCTCAGTACCAGCACCTTCTGCGGCTTGAA GATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACCAAAGAATGCAGGAACCAA CGGGGCGATATCAACAGGAAAGACTTCAAATGCAAACTCGGTCTCTTAG

2018267601 21 Nov 2018

SEQ ID NO: 66

Cuphea heterophylla (Cht) FATB2g (A6T, A16V, S17P, G76D, R97L, H124L, I132S, S1431, G152S, A157T, H165L, T21 IN, G414A variant) coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGTGGCCGCCACCGCCTCCTCCGCCTTCTTCCCCGTGCCCGTGCCCGGCACCTCCC

CCAAGCCCGGCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTC

CAACCCCTCCGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGG

CTCCGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCC CCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGTCCCGCCTGCTGACCGCCATCTCCA

CCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAAGTCCAAGCGCCCCG

ACATGCTGGTGGACTGGTTCGGCCTGGAGTCCATCGTGCAGGACGGCCTGGTGTTCCGCG

AGATCTACTCCATCCGCTCCTACGAGATCTCCGCCGACCGCACCACCTCCATCGAGACCGT GATGAACCTGCTGCAGGAGACCTCCCTGAACCACTGCAAGTCCATGGGCATCCTGAACGA

CGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGAT

GCAGATCCTGGTGAACCGCTACCCCAACTGGGGCGACACCGTGGAGATCAACTCCTGGTT

CTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACTGGCTGATCTCCGACTGCAACACCGG CGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCAGAAGACCCGCCGCTT

CTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCCCCCCC

CGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCAT

CTGCAAGGGCCTGACCCCCGAGTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGT

GAAGTACATCGGCTGGATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCT GTGCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGT

GACCGCCATGGACCCCTCCAAGGTGGGCGACCGCTCCCAGTACCAGCACCTGCTGCGCCT GGAGGACGGCACCGACATCATGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCA

CCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGCCAACTCCGTGTCCTGA

SEQ ID NO: 67

Cuphea heterophylla (Cht) FATB3aamino acid sequence

MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKINGSSVSL KSCSLKTHEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPF

GLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNEGFGRTPEMY KRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSV WVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKLPKLDEKTADSIRKGLTPKWNDL DVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKGFGPQFQ HLLRLEDGGEIVKGRTEWRPKTAGINGTIASGETSPGNS*

SEQ ID NO: 68

Cuphea heterophylla (Cht) FATB3a coding DNA sequence

ATGGTGGCCACCGCTGCAAGTTCTGCATTCTTCCCGGTGCCGTCCCCGGACACCTCCTCTA GACCGGGAAAGCTCGGAAATGGGTCATCAAGCTTGAGGCCCCTCAAGCCCAAATTTGTTG

CCAATGCTGGGCTGCAGGTTAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTCCT

CGGTCAGTCTAAAGTCTTGCAGTCTCAAGACTCATGAAGACACTCCTTCAGCTCCTCCTCC

GCGGACTTTTATCAACCAGTTGCCTGATTGGAGCATGCTTCTTGCTGCAATCACTACTGTC

TTCTTGGCAGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCAAAGAGGCCTGACATG

CTTGTGGACCCGTTCGGATTGGGAAGGATTGTTCAGGATGGGCTTGTGTTCAGGCAGAATT TTTCGATTAGGTCCTATGAAATAGGCGCTGATCGCACTGCATCCATAGAGACGGTGATGA

ACCACTTGCAGGAAACGGCTCTCAATCATGTTAAGAGTGCGGGGCTTCTTAATGAAGGCT

TTGGTCGTACTCCTGAGATGTATAAAAGGGACCTTATTTGGGTTGTCGCGAAAATGCAGGT CATGGTTAACCGCTATCCTACTTGGGGTGACACGGTTGAAGTGAATACTTGGGTTGCCAAG

TCAGGGAAAAATGGTATGCGTCGTGATTGGCTCATAAGTGATTGCAATACAGGAGAAATT

CTTACAAGGGCATCAAGTGTGTGGGTCATGATGAATCAAAAGACAAGAAAATTGTCAAAG

ATTCCAGATGAGGTTCGGCATGAGATAGAGCCTCATTTTGTGGACTCTGCTCCCGTCATTG

AAGACGATGACTGGAAACTTCCCAAGCTGGATGAGAAAACTGCTGACTCCATCCGCAAGG

GTCTAACTCCGAAGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTACA

TTGGGTGGATTCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGTTCCCT

2018267601 21 Nov 2018

TACCCTGGAATACAGGCGGGAATGCGGAAGGGAGAGTGTGCTGGAGTCCCTCACTGCTGT

GGACCCCTCTGGAAAGGGCTTTGGGCCCCAGTTTCAGCACCTTCTGAGGCTTGAGGATGG AGGTGAGATCGTAAAGGGGAGAACTGAGTGGCGACCCAAGACTGCAGGTATCAATGGGA CGATTGCATCTGGGGAGACCTCACCTGGAAACTCTTAG

SEQ ID NO: 69

Cuphea heterophylla (Cht) FATB3a coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCCCCGACACCTCCTCCCG

CCCCGGCAAGCTGGGCAACGGCTCCTCCTCCCTGCGCCCCCTGAAGCCCAAGTTCGTGGCC 10 AACGCCGGCCTGCAGGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCC

GTGTCCCTGAAGTCCTGCTCCCTGAAGACCCACGAGGACACCCCCTCCGCCCCCCCCCCCC

GCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGGCCGCCATCACCACCGTGTT

CCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAAGCGCCCCGACATGCT GGTGGACCCCTTCGGCCTGGGCCGCATCGTGCAGGACGGCCTGGTGTTCCGCCAGAACTT 15 CTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAA

CCACCTGCAGGAGACCGCCCTGAACCACGTGAAGTCCGCCGGCCTGCTGAACGAGGGCTT

CGGCCGCACCCCCGAGATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGT

GATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCCAA

GTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGAT 20 CCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGACCCGCAAGCTGTCCAA

GATCCCCGACGAGGTGCGCCACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCCGTGAT

CGAGGACGACGACTGGAAGCTGCCCAAGCTGGACGAGAAGACCGCCGACTCCATCCGCA

AGGGCCTGACCCCCAAGTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAG

TACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGC 25 TCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACC

GCCGTGGACCCCTCCGGCAAGGGCTTCGGCCCCCAGTTCCAGCACCTGCTGCGCCTGGAG GACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGACCGCCGGCATCAA CGGCACCATCGCCTCCGGCGAGACCTCCCCCGGCAACTCCTGA

SEQ ID NO: 70

Cuphea heterophylla (Cht) FATB3b (C67G, H72Q, L128F, N179I variant) amino acid sequence

MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKINGSSVSL KSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPF GFGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLIEGFGRTPEMYK

RDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSVW

VMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKLPKLDEKTADSIRKGLTPKWNDLD

VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKGFGPQFQH LLRLEDGGEIVKGRTEWRPKTAGINGTIASGETSPGNS*

SEQ ID NO: 71

Cuphea heterophylla (Cht) FATB3b (C67G, H72Q, L128F, N179I variant) coding DNA sequence

ATGGTGGCCACCGCTGCAAGTTCTGCATTCTTCCCGGTGCCATCCCCGGACACCTCCTCTA

GACCGGGAAAGCTCGGAAATGGGTCATCAAGCTTGAGGCCCCTCAAGCCCAAATTTGTTG CCAATGCTGGGCTGCAGGTTAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTCCT

CGGTCAGTCTAAAGTCTGGCAGTCTCAAGACTCAGGAAGACACTCCTTCGGCTCCTCCTCC

GCGGACTTTTATCAACCAGTTGCCTGATTGGAGCATGCTTCTTGCTGCAATCACTACTGTC

TTCTTGGCAGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCAAAGAGGCCTGACATG

CTTGTGGACCCGTTCGGATTTGGAAGGATTGTTCAGGATGGGCTTGTGTTCAGGCAGAATT TTTCGATTAGGTCCTATGAAATAGGCGCTGATCGCACTGCATCTATAGAGACGGTGATGA

ACCACTTGCAGGAAACGGCTCTCAATCATGTTAAGAGTGCGGGGCTTCTTATTGAAGGCTT

TGGTCGTACTCCTGAGATGTATAAAAGGGACCTTATTTGGGTTGTCGCGAAAATGCAGGTC 5 0 ATGGTT AACCGCT ATCCTACTTGGGGTGAC ACGGTTGAAGTGAAT ACTTGGGTTGCC AAGT

CAGGGAAAAATGGTATGCGTCGTGATTGGCTCATAAGTGATTGCAATACAGGAGAAATTC

TTACTAGAGCATCAAGTGTGTGGGTCATGATGAATCAAAAGACAAGAAAATTGTCAAAGA

2018267601 21 Nov 2018

TTCCAGATGAGGTTCGGCATGAGATAGAGCCTCATTTTGTGGACTCTGCTCCCGTCATTGA

AGACGATGACTGGAAACTTCCCAAGCTGGATGAGAAAACTGCTGACTCCATCCGCAAGGG

TCTAACTCCGAAGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTACAT

TGGGTGGATTCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGTTCCCTT

ACCCTGGAATACAGGCGGGAATGCGGAAGGGAGAGTGTGCTGGAGTCCCTCACTGCTGTG

GACCCCTCTGGAAAGGGCTTTGGGCCCCAGTTTCAGCACCTTCTGAGGCTTGAGGATGGA GGTGAGATCGTAAAGGGGAGAACTGAGTGGCGACCCAAGACTGCAGGTATCAATGGGAC GATTGCATCTGGGGAGACCTCACCTGGAAACTCTTAG

SEQ ID NO: 72

Cuphea heterophylla (Cht) FATB3b (C67G, H72Q, L128F, N179I variant) coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCCCCGACACCTCCTCCCG

CCCCGGCAAGCTGGGCAACGGCTCCTCCTCCCTGCGCCCCCTGAAGCCCAAGTTCGTGGCC AACGCCGGCCTGCAGGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCC

GTGTCCCTGAAGTCCGGCTCCCTGAAGACCCAGGAGGACACCCCCTCCGCCCCCCCCCCCC

GCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGGCCGCCATCACCACCGTGTT

CCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAAGCGCCCCGACATGCT

GGTGGACCCCTTCGGCTTCGGCCGCATCGTGCAGGACGGCCTGGTGTTCCGCCAGAACTTC TCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAAC 20 CACCTGCAGGAGACCGCCCTGAACCACGTGAAGTCCGCCGGCCTGCTGATCGAGGGCTTC

GGCCGCACCCCCGAGATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGTG

ATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCCAAG

TCCGGCAAGAACGGCATGCGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGATC CTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGACCCGCAAGCTGTCCAAG 25 ATCCCCGACGAGGTGCGCCACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCCGTGATC

GAGGACGACGACTGGAAGCTGCCCAAGCTGGACGAGAAGACCGCCGACTCCATCCGCAA GGGCCTGACCCCCAAGTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGT

ACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGCT

CCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCG 3 0 CCGTGGACCCCTCCGGCAAGGGCTTCGGCCCCC AGTTCCAGCACCTGCTGCGCCTGGAGG

ACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGACCGCCGGCATCAAC

GGCACCATCGCCTCCGGCGAGACCTCCCCCGGCAACTCCTGA

SEQ ID NO: 73

Cuphea viscosissima (Cvis) FATB1 amino acid sequence

MVAAAATSAFFPVPAPGTSPKPGKSGNWPSSLSPTFKPKSIPNGGFQVKANASAHPKANGSAV

NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRPDMLVD SVGLKSIVRDGLVSRHSFSIRSYEIGADRTASIETLMNHLQETTINHCKSLGLHNDGFGRTPGM CKNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSQSGKIGMASDWLISDCNTGEILIRATSVW AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDQKLRKFDVKTGDSIRKGLTPRWNDLD 40 VNQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAVDPSENGGRSQYK

HLLRLEDGTDIVKSRTEWRPKNAGTNGAISTSTAKTSNGNSVS

SEQ ID NO: 74

Cuphea viscosissima (Cvis) FATB1 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCGCCGCCACCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCTCCCCCA

AGCCCGGCAAGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCACCTTCAAGCCCAAGTCCAT

CCCCAACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC

CGCCGTGAACCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCC

CCCCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGACCGCCATCACCACCG

TGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCGACA 50 TGCTGGTGGACTCCGTGGGCCTGAAGTCCATCGTGCGCGACGGCCTGGTGTCCCGCCACTC

CTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATG

AACCACCTGCAGGAGACCACCATCAACCACTGCAAGTCCCTGGGCCTGCACAACGACGGC

2018267601 21 Nov 2018

TTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCAG ATCATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACACCTGGTTCTCC CAGTCCGGCAAGATCGGCATGGCCTCCGACTGGCTGATCTCCGACTGCAACACCGGCGAG ATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCTCCC GCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTTCGTGGACTCCCCCCACGTGAT CGAGGACAACGACCAGAAGCTGCGCAAGTTCGACGTGAAGACCGGCGACTCCATCCGCA AGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGT ACATCGGCTGGATCCTGGAGTCCATGCCCATCGAGGTGCTGGAGACCCAGGAGCTGTGCT CCCTGACCGTGGAGTACCGCCGCGAGTGCGGCATGGACTCCGTGCTGGAGTCCGTGACCG CCGTGGACCCCTCCGAGAACGGCGGCCGCTCCCAGTACAAGCACCTGCTGCGCCTGGAGG ACGGCACCGACATCGTGAAGTCCCGCACCGAGTGGCGCCCCAAGAACGCCGGCACCAAC GGCGCCATCTCCACCTCCACCGCCAAGACCTCCAACGGCAACTCCGTGTCCTGA

SEQ ID NO: 75

Cuphea viscosissima (Cvis) FATB2 amino acid sequence

MVATAASSAFFPVPSADTSSRPGKLGNGPSSFSPLKPKSIPNGGLQVKASASAPPKINGSSVGLK SGGLKTHDDAPSAPPPRTFINQLPDWSMLLAAITTAFLAAEKQWMMLDRKPKRLDMLEDPFG LGRWQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKTAGLSNDGFGRTPEMYK RDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSVW VMMNQKTRKLSKIPDEVRREIEPHFVDSAPVIEDDDRKLPKLDEKSADSIRKGLTPRWNDLDV NQHVNNAKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGEGYGSQFQHL LRLEDGGEIVKGRTEWRPKNAGINGWPSEESSPGDYS

SEQ ID NO: 76

Cuphea viscosissima (Cvis) FATB2 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCGCCGACACCTCCTCCC GCCCCGGCAAGCTGGGCAACGGCCCCTCCTCCTTCTCCCCCCTGAAGCCCAAGTCCATCCC CAACGGCGGCCTGCAGGTGAAGGCCTCCGCCTCCGCCCCCCCCAAGATCAACGGCTCCTC CGTGGGCCTGAAGTCCGGCGGCCTGAAGACCCACGACGACGCCCCCTCCGCCCCCCCCCC CCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGGCCGCCATCACCACCGCC TTCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACCGCAAGCCCAAGCGCCTGGACATG CTGGAGGACCCCTTCGGCCTGGGCCGCGTGGTGCAGGACGGCCTGGTGTTCCGCCAGAAC TTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGA ACCACCTGCAGGAGACCGCCCTGAACCACGTGAAGACCGCCGGCCTGTCCAACGACGGCT TCGGCCGCACCCCCGAGATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGG TGATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCCA AGTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGA TCCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGACCCGCAAGCTGTCCA AGATCCCCGACGAGGTGCGCCGCGAGATCGAGCCCCACTTCGTGGACTCCGCCCCCGTGA TCGAGGACGACGACCGCAAGCTGCCCAAGCTGGACGAGAAGTCCGCCGACTCCATCCGCA AGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGCCAAGT ACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGCT CCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCG CCGTGGACCCCTCCGGCGAGGGCTACGGCTCCCAGTTCCAGCACCTGCTGCGCCTGGAGG ACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCATCAAC GGCGTGGTGCCCTCCGAGGAGTCCTCCCCCGGCGACTACTCCTGA

SEQ ID NO: 77

Cuphea viscosissima (Cvis) FATB3 amino acid sequence

MVAAAASSAFFSFPTPGTSPKPGKFGNWPSSLSIPFNPKSNHNGGIQVKANASAHPKANGSAVS LKAGSLETQEDTSSPSPPPRTFISQLPDWSMLVSAITTVFVAAEKQWTMLDRKSKRPDVLVEPF VQDGVSFRQSFSIRSYEIGVDRTASIETLMNIFQETSLNHCKSLGLLNDGFGRTPEMCKRDLIW WTKMQIEVNRYPTWGDTIEVTTWVSESGKNGMSRDWLISDCHSGEILIRATSVWAMMNQK TRRLSKIPDEVRQEIVPYFVDSAPVIEDDRKLHKLDVKTGDSIRNGLTPRWNDFDVNQHVNNV

2018267601 21 Nov 2018

KYIAWLLKSVPTEVFETQELCGLTLEYRRECRRDSVLESVTAMDPSKEGDRSLYQHLLRLENG ADIALGRTEWRPKNAGATGAVSTGKTSNGNSVS

SEQ ID NO: 78

Cuphea viscosissima (Cvis) FATB3 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCTCCTTCCCCACCCCCGGCACCTCCCCCAA

GCCCGGCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCATCCCCTTCAACCCCAAGTCCAAC

CACAACGGCGGCATCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCC GCCGTGTCCCTGAAGGCCGGCTCCCTGGAGACCCAGGAGGACACCTCCTCCCCCTCCCCCC

CCCCCCGCACCTTCATCTCCCAGCTGCCCGACTGGTCCATGCTGGTGTCCGCCATCACCAC

CGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCGA

CGTGCTGGTGGAGCCCTTCGTGCAGGACGGCGTGTCCTTCCGCCAGTCCTTCTCCATCCGC TCCTACGAGATCGGCGTGGACCGCACCGCCTCCATCGAGACCCTGATGAACATCTTCCAG

GAGACCTCCCTGAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGGCTTCGGCCGCACC

CCCGAGATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGATGCAGATCGAGGTGAAC

CGCTACCCCACCTGGGGCGACACCATCGAGGTGACCACCTGGGTGTCCGAGTCCGGCAAG

AACGGCATGTCCCGCGACTGGCTGATCTCCGACTGCCACTCCGGCGAGATCCTGATCCGC

GCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCCTGTCCAAGATCCCCGAC

GAGGTGCGCCAGGAGATCGTGCCCTACTTCGTGGACTCCGCCCCCGTGATCGAGGACGAC

CGCAAGCTGCACAAGCTGGACGTGAAGACCGGCGACTCCATCCGCAACGGCCTGACCCCC

CGCTGGAACGACTTCGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGCCTGGCTG

CTGAAGTCCGTGCCCACCGAGGTGTTCGAGACCCAGGAGCTGTGCGGCCTGACCCTGGAG TACCGCCGCGAGTGCCGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGGACCCCTCC

AAGGAGGGCGACCGCTCCCTGTACCAGCACCTGCTGCGCCTGGAGAACGGCGCCGACATC GCCCTGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCGCCACCGGCGCCGTGTCCACC

GGCAAGACCTCCAACGGCAACTCCGTGTCCTGA

SEQ ID NO: 79

Cuphea calcarata (Ccalc) FATB1 amino acid sequence

MVAASASSAFFSVPTPGTSPKPGKFGNWPSSLSVPFKPRSNNSGGFQVKANASAHPKANGSAV SLKSGSLETQEDNSSSSRPPRTFIKQLPDWSMLLSAITTVFVAAEKQWTMFDRKSKRSDMLVD

PFVVDRIVQDGVLFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSMGLLYEGFGRTPEMC

KRDLIWWTKIHIKVNRYPTWGDTIEVTTWVSESGKNGMGRDWLISDCHTGEILIRATSVWA

MMNQTTRRLSKFPYELRQEIAPHFVDSDPVIEDNRRLLNFDVKTGDSIRKGLTPRWNDLDVNQ HVNNVKYIGWILESVPTEVFDTRELCGLTLEYRQECGRGSVLESVTAMDPSKEGDRSLYQHLL RLEDGTDIVKGRTEWRPKNAGTNGPVSTRKTTNGSSVS

SEQ ID NO: 80

Cuphea calcarata (Ccalc) FATB1 coding DNA sequence

ATGGTGGCTGCTTCAGCAAGTTCTGCATTCTTCTCCGTCCCAACCCCGGGAACCTCTCCTA AACCCGGGAAGTTCGGCAATTGGCCATCGAGCTTGAGCGTCCCATTCAAGCCCAGATCAA

ACAACAGTGGCGGCTTTCAGGTTAAGGCAAACGCCAGTGCTCATCCTAAGGCTAACGGTT

CTGCAGTAAGTCTAAAGTCTGGGAGCCTCGAGACTCAGGAGGACAATTCGTCGTCGTCTC

GTCCTCCTCGGACTTTCATTAAACAGTTGCCGGACTGGAGTATGCTTCTTTCCGCGATCAC AACCGTCTTCGTGGCGGCTGAGAAGCAGTGGACGATGTTTGATCGGAAATCTAAGAGGTC

TGACATGCTCGTGGACCCGTTTGTGGTTGACAGGATTGTTCAGGATGGGGTTCTGTTCAGA CAGAGTTTTTCGATTAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATTGAGACGC

TGATGAACATCTTCCAGGAAACATCTCTCAATCATTGTAAGAGTATGGGTCTTCTCTATGA

AGGCTTTGGTCGTACTCCTGAGATGTGTAAGAGGGACCTCATTTGGGTGGTTACGAAAAT

ACATATCAAGGTGAATCGCTATCCGACTTGGGGTGATACTATCGAGGTCACTACTTGGGTC

TCCGAGTCGGGCAAAAACGGTATGGGTCGCGATTGGCTGATAAGTGATTGCCATACAGGA

GAAATTCTTATAAGAGCAACGAGTGTGTGGGCTATGATGAATCAAACGACGAGAAGATTG

TCGAAATTTCCATATGAGCTTCGACAGGAGATAGCGCCACATTTTGTGGACTCGGATCCTG

TCATTGAAGACAATCGAAGATTGCTCAACTTTGATGTGAAGACGGGTGATTCCATTCGCA

AGGGTCTAACTCCAAGGTGGAATGACTTGGATGTCAATCAGCACGTTAACAATGTGAAGT

2018267601 21 Nov 2018

ACATTGGGTGGATTCTCGAGAGTGTTCCAACAGAAGTTTTCGATACCCGGGAGCTATGCG

GCCTCACCCTTGAGTATAGGCAGGAATGCGGAAGAGGAAGTGTGCTGGAGTCCGTGACCG CTATGGATCCCTCAAAAGAGGGAGACCGGTCTCTGTACCAGCACCTTCTTCGGCTTGAGG

ATGGGACTGATATCGTGAAGGGCAGAACCGAGTGGCGGCCAAAGAATGCAGGAACCAAT 5 GGGCCAGTATCAACAAGAAAGACTACAAATGGAAGCTCAGTCTCTTAG

SEQ ID NO: 81

Cuphea calcarata (Ccalc) FATB1 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCTCCGCCTCCTCCGCCTTCTTCTCCGTGCCCACCCCCGGCACCTCCCCCA AGCCCGGCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCGTGCCCTTCAAGCCCCGCTCCAA

CAACTCCGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC

CGCCGTGTCCCTGAAGTCCGGCTCCCTGGAGACCCAGGAGGACAACTCCTCCTCCTCCCGC CCCCCCCGCACCTTCATCAAGCAGCTGCCCGACTGGTCCATGCTGCTGTCCGCCATCACCA

CCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGTTCGACCGCAAGTCCAAGCGCTCCG ACATGCTGGTGGACCCCTTCGTGGTGGACCGCATCGTGCAGGACGGCGTGCTGTTCCGCC

AGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCT

GATGAACATCTTCCAGGAGACCTCCCTGAACCACTGCAAGTCCATGGGCCTGCTGTACGA

GGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGAT

CCACATCAAGGTGAACCGCTACCCCACCTGGGGCGACACCATCGAGGTGACCACCTGGGT GTCCGAGTCCGGCAAGAACGGCATGGGCCGCGACTGGCTGATCTCCGACTGCCACACCGG

CGAGATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGACCACCCGCCGCCT

GTCCAAGTTCCCCTACGAGCTGCGCCAGGAGATCGCCCCCCACTTCGTGGACTCCGACCCC GTGATCGAGGACAACCGCCGCCTGCTGAACTTCGACGTGAAGACCGGCGACTCCATCCGC

AAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAA GTACATCGGCTGGATCCTGGAGTCCGTGCCCACCGAGGTGTTCGACACCCGCGAGCTGTG

CGGCCTGACCCTGGAGTACCGCCAGGAGTGCGGCCGCGGCTCCGTGCTGGAGTCCGTGAC

CGCCATGGACCCCTCCAAGGAGGGCGACCGCTCCCTGTACCAGCACCTGCTGCGCCTGGA GGACGGCACCGACATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCACCA

ACGGCCCCGTGTCCACCCGCAAGACCACCAACGGCTCCTCCGTGTCCTGA

SEQ ID NO: 82

Cuphea painteri (Cpai) FATB1 amino acid sequence

MVAAAATSAFFPVPAPGTSPNPRKFGSWPSSLSPSLPKSIPNGGFQVKANASAHPKANGSAVSL KSGSLNTQENTSSSPPPRTFLHQLPDWSRLLTAITTVFVKSKRPDMHDRKSKRPDMLVDLFGLE SSVQDALVFRQSFSIRSYEIGTDRTASIETLMNHLQETSLNHCKSTGILLDGFGRTLEMCKRELI

WWIKMQIQVNRYPAWGDTVEINTRFSRLGKIGMGRDWLISDCNTGEILIRATSEYAMMNQK TRRLSKLPYEVHQEIAPLFVDSPPVIEDNDLKVHKFEVKTGDSIQKGLSPGWNDLDVNQHVSN VKYIGWILESMPTEVLETQELCSLALEYRRECGRDSVLESVTAMDPSKVGGRSQYQHLLRLED GTAIVNGITEWRPKNAGANGAISTGKTSNGNSVS

SEQ ID NO: 83

Cuphea painteri (Cpai) FATB1 coding DNA sequence

ATGGTGGCTGCTGCAGCAACTTCTGCATTCTTCCCTGTTCCAGCCCCGGGAACCTCCCCAA ATCCCAGGAAATTCGGAAGTTGGCCATCGAGCTTGAGCCCTTCCTTGCCCAAGTCAATCCC

CAATGGCGGATTTCAGGTAAAGGCAAATGCCAGTGCCCATCCGAAGGCTAACGGTTCTGC

AGTTAGTCTAAAGTCTGGCAGCCTCAACACTCAGGAGAACACTTCGTCGTCCCCTCCTCCT

CGGACTTTCCTTCACCAGTTGCCTGATTGGAGTAGGCTTCTGACTGCAATCACGACCGTGT TCGTGAAATCTAAGAGGCCTGACATGCATGATCGGAAATCTAAGAGGCCTGACATGCTGG

TGGACTTGTTTGGGTTGGAAAGTAGTGTTCAGGATGCGCTCGTGTTCAGACAGAGTTTTTC GATTAGGTCTTATGAAATAGGCACTGATCGAACAGCCTCTATAGAGACGCTGATGAACCA

0 CTTGC AGGAAAC ATCTCTCAATC ATTGT AAAAGT ACCGGTATTCTCCTTGACGGCTTCGGT

CGTACTCTTGAGATGTGTAAAAGGGAACTCATTTGGGTGGTAATAAAAATGCAAATTCAG GTGAATCGCTATCCAGCATGGGGCGATACTGTCGAGATCAATACCCGGTTCTCCCGGTTGG

GGAAAATTGGTATGGGTCGCGATTGGCTAATAAGTGATTGCAACACAGGAGAAATTCTAA

TAAGAGCAACGAGCGAGTATGCCATGATGAATCAAAAGACGAGAAGACTCTCAAAACTT

2018267601 21 Nov 2018

CCATACGAGGTTCACCAGGAGATAGCGCCTCTTTTTGTCGACTCTCCTCCTGTGATTGAAG ACAATGATCTGAAAGTGCATAAATTTGAAGTGAAGACTGGTGATTCCATTCAAAAGGGTC TATCCCCGGGGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTACATTG GGTGGATTCTCGAGAGTATGCCAACAGAAGTTTTGGAGACCCAGGAGCTATGCTCTCTCG CCCTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCCGTGACCGCAATGG ATCCCTCAAAAGTTGGAGGCCGTTCTCAGTACCAGCACCTTCTGCGGCTTGAGGATGGGA CTGCTATCGTGAACGGCATAACTGAGTGGCGGCCGAAGAATGCAGGAGCTAATGGGGCG ATATCAACGGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG

SEQ ID NO: 84

Cuphea painteri (Cpai) FATB1 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCGCCGCCACCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCTCCCCCA ACCCCCGCAAGTTCGGCTCCTGGCCCTCCTCCCTGTCCCCCTCCCTGCCCAAGTCCATCCCC AACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCC GTGTCCCTGAAGTCCGGCTCCCTGAACACCCAGGAGAACACCTCCTCCTCCCCCCCCCCCC GCACCTTCCTGCACCAGCTGCCCGACTGGTCCCGCCTGCTGACCGCCATCACCACCGTGTT CGTGAAGTCCAAGCGCCCCGACATGCACGACCGCAAGTCCAAGCGCCCCGACATGCTGGT GGACCTGTTCGGCCTGGAGTCCTCCGTGCAGGACGCCCTGGTGTTCCGCCAGTCCTTCTCC ATCCGCTCCTACGAGATCGGCACCGACCGCACCGCCTCCATCGAGACCCTGATGAACCAC CTGCAGGAGACCTCCCTGAACCACTGCAAGTCCACCGGCATCCTGCTGGACGGCTTCGGC CGCACCCTGGAGATGTGCAAGCGCGAGCTGATCTGGGTGGTGATCAAGATGCAGATCCAG GTGAACCGCTACCCCGCCTGGGGCGACACCGTGGAGATCAACACCCGCTTCTCCCGCCTG GGCAAGATCGGCATGGGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGATCCTG ATCCGCGCCACCTCCGAGTACGCCATGATGAACCAGAAGACCCGCCGCCTGTCCAAGCTG CCCTACGAGGTGCACCAGGAGATCGCCCCCCTGTTCGTGGACTCCCCCCCCGTGATCGAG GACAACGACCTGAAGGTGCACAAGTTCGAGGTGAAGACCGGCGACTCCATCCAGAAGGG CCTGTCCCCCGGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACAT CGGCTGGATCCTGGAGTCCATGCCCACCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCT GGCCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCAT GGACCCCTCCAAGGTGGGCGGCCGCTCCCAGTACCAGCACCTGCTGCGCCTGGAGGACGG CACCGCCATCGTGAACGGCATCACCGAGTGGCGCCCCAAGAACGCCGGCGCCAACGGCGC CATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA

SEQ ID NO: 85

Cuphea hookeriana (Chook) FATB4 amino acid sequence

MVAAAATSAFFPVPAPGTSPNPRKFGSWPSSLSPSLPNSIPNGGFQVKANASAHPKANGSAVSL KSGSLNTQENTSSSPPPRTFLHQLPDWSRLLTAITTVFVKSKRPDMHDRKSKRPDMLVDLFGLE SSVQDALVFRQRFSIRSYEIGTDRTASMETLMNHLQETSLNHCKSTGILLDGFGRTLEMCKREL IWWIKMQIQVNRYPAWGDTVEINTRFSRLGKIGMGRDWLISDCNTGEILIRATSEYAMMNQK TRRLSKLPYEVRQEIAPLFVDSPPVIEDNDLKVHKFEVKTGDSIHKGLTPGWNDLDVNQHVNN VKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAMDPSGGGYGSQFQHLLRLEDG GEIVKGRTEWRPKNGVINGWPTGESSPGDYS

SEQ ID NO: 86

Cuphea hookeriana (Chook) FATB4 coding DNA sequence

ATGGTGGCTGCTGCAGCAACTTCTGCATTCTTCCCTGTTCCAGCCCCGGGAACCTCCCCTA ATCCCAGGAAATTCGGAAGTTGGCCATCGAGCTTGAGCCCTTCCTTGCCCAACTCAATCCC CAATGGCGGATTTCAGGTAAAGGCAAATGCCAGTGCCCATCCGAAGGCTAACGGTTCTGC AGTTAGTCTAAAGTCTGGCAGCCTCAACACTCAGGAGAACACTTCGTCGTCCCCTCCTCCT CGGACTTTCCTTCACCAGTTGCCTGATTGGAGTAGGCTTCTGACTGCAATCACGACCGTGT TCGTGAAATCTAAGAGGCCTGACATGCATGATCGGAAATCTAAGAGGCCTGACATGCTGG TGGACTTGTTTGGGTTGGAGAGTAGTGTTCAGGATGCGCTCGTGTTCAGACAGAGATTTTC GATTAGGTCTTATGAAATAGGCACTGATCGAACAGCCTCTATGGAGACGCTGATGAACCA CTTGCAGGAAACATCTCTCAATCATTGTAAAAGTACCGGTATTCTCCTTGACGGCTTCGGT CGTACTCTTGAGATGTGTAAAAGGGAACTCATTTGGGTGGTAATAAAAATGCAGATTCAG

2018267601 21 Nov 2018

GTGAATCGCTATCCAGCATGGGGCGATACTGTCGAGATCAATACCCGGTTCTCCCGGTTGG

GGAAAATTGGTATGGGTCGCGATTGGCTAATAAGTGATTGCAACACAGGAGAAATTCTTA TAAGAGCAACGAGCGAGTATGCCATGATGAATCAAAAGACGAGAAGACTCTCAAAACTT

CCATACGAGGTTCGCCAGGAGATAGCGCCTCTTTTTGTCGACTCTCCTCCTGTGATTGAAG

ACAATGATCTGAAAGTGCATAAATTTGAAGTGAAGACTGGTGATTCCATTCACAAGGGTC

TAACTCCGGGGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTACATCG

GGTGGATTCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGTTCCCTTAC

TCTGGAATACAGGCGGGAATGTGGAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTATGGA

TCCCTCTGGAGGGGGTTATGGGTCCCAGTTTCAGCACCTTCTGCGGCTTGAGGATGGAGGT 10 GAGATCGTGAAGGGGAGAACCGAGTGGCGACCCAAGAATGGTGTAATCAATGGGGTGGT

ACCAACCGGGGAGTCCTCACCTGGAGACTACTCTTAG

SEQ ID NO: 87

Cuphea hookeriana (Chook) FATB4 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCGCCGCCACCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCTCCCCCA

ACCCCCGCAAGTTCGGCTCCTGGCCCTCCTCCCTGTCCCCCTCCCTGCCCAACTCCATCCCC

AACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCC

GTGTCCCTGAAGTCCGGCTCCCTGAACACCCAGGAGAACACCTCCTCCTCCCCCCCCCCCC 20 GCACCTTCCTGCACCAGCTGCCCGACTGGTCCCGCCTGCTGACCGCCATCACCACCGTGTT

CGTGAAGTCCAAGCGCCCCGACATGCACGACCGCAAGTCCAAGCGCCCCGACATGCTGGT GGACCTGTTCGGCCTGGAGTCCTCCGTGCAGGACGCCCTGGTGTTCCGCCAGCGCTTCTCC

ATCCGCTCCTACGAGATCGGCACCGACCGCACCGCCTCCATGGAGACCCTGATGAACCAC

CTGCAGGAGACCTCCCTGAACCACTGCAAGTCCACCGGCATCCTGCTGGACGGCTTCGGC

CGCACCCTGGAGATGTGCAAGCGCGAGCTGATCTGGGTGGTGATCAAGATGCAGATCCAG

GTGAACCGCTACCCCGCCTGGGGCGACACCGTGGAGATCAACACCCGCTTCTCCCGCCTG

GGCAAGATCGGCATGGGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGATCCTG

ATCCGCGCCACCTCCGAGTACGCCATGATGAACCAGAAGACCCGCCGCCTGTCCAAGCTG

CCCTACGAGGTGCGCCAGGAGATCGCCCCCCTGTTCGTGGACTCCCCCCCCGTGATCGAG

0 GAC AACGACCTGAAGGTGC AC AAGTTCGAGGTGAAGACCGGCGACTCC ATCC AC AAGGG

CCTGACCCCCGGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACAT

CGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCT

GACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCGCCAT

GGACCCCTCCGGCGGCGGCTACGGCTCCCAGTTCCAGCACCTGCTGCGCCTGGAGGACGG 3 5 CGGCGAGATCGTGAAGGGCCGC ACCGAGTGGCGCCCCAAGAACGGCGTGATCAACGGCG

TGGTGCCCACCGGCGAGTCCTCCCCCGGCGACTACTCCTGA

SEQ ID NO: 88

Cuphea avigera var. pulcherrima (Ca) FATB1 amino acid sequence

MVAAAASSAFFSVPVPGTSPKPGKFRIWPSSLSPSFKPKPIPNGGLQVKANSRAHPKANGSAVS LKSGSLNTQEDTSSSPPPRTFLHQLPDWSRLLTAITTVFVKSKRPDMHDRKSKRPDMLMDSFG LESIVQEGLEFRQSFSIRSYEIGTDRTASIETLMNYLQETSLNHCKSTGILLDGFGRTPEMCKRDL IWWTKMKIKVNRYPAWGDTVEINTWFSRLGKIGKGRDWLISDCNTGEILIRATSAYATMNQ KTRRLSKLPYEVHQEIAPLFVDSPPVIEDNDLKLHKFEVKTGDSIHKGLTPGWNDLDVNQHVS

NVKYIGWILESMPTEVLETQELCSLALEYRRECGRDSVLESVTAMDPTKVGGRSQYQHLLRLE

DGTDIVKCRTEWRPKNPGANGAISTGKTSNGNSVS

SEQ ID NO: 89

Cuphea avigera var. pulcherrima (Ca) FATB1 coding DNA sequence

ATGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCTCTGTTCCAGTCCCGGGAACCTCTCCTA

AACCCGGGAAGTTCAGAATTTGGCCATCGAGCTTGAGCCCTTCCTTCAAGCCCAAGCCGA

TCCCCAATGGTGGATTGCAGGTTAAGGCAAATTCCAGGGCACATCCGAAGGCTAACGGTT

CTGCAGTTAGTCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCCTCC

TCCTCGGACTTTCCTTCACCAGTTGCCTGATTGGAGTAGGCTTCTGACTGCAATCACGACC

5 GTGTTCGTGAAATCTAAGAGGCCTGAC ATGCATGATCGGAAATCTAAGAGGCCTGACATG

2018267601 21 Nov 2018

CTGATGGACTCGTTTGGGTTGGAGAGTATTGTTCAAGAAGGGCTCGAGTTCAGACAGAGT TTTTCGATTAGGTCTTATGAAATAGGCACTGATCGAACAGCCTCTATAGAGACGCTGATGA ACTACTTGCAGGAAACATCTCTCAATCATTGTAAGAGTACCGGTATTCTCCTTGACGGCTT TGGTCGTACTCCTGAGATGTGTAAAAGGGACCTCATTTGGGTGGTAACAAAAATGAAGAT

CAAGGTGAATCGCTATCCAGCTTGGGGCGATACTGTCGAGATCAATACCTGGTTCTCCCGG

TTGGGGAAAATCGGAAAGGGTCGCGATTGGCTAATAAGTGATTGCAACACAGGAGAAATT CTTATAAGAGCAACGAGCGCGTATGCCACGATGAATCAAAAGACGAGAAGACTCTCAAA

ACTTCCATACGAGGTTCACCAGGAGATAGCGCCTCTCTTTGTCGACTCTCCTCCTGTCATT GAAGACAATGATCTGAAATTGCATAAGTTTGAAGTGAAGACTGGTGATTCCATTCACAAG

GGTCTAACTCCGGGGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTAC ATTGGGTGGATTCTCGAGAGTATGCCAACAGAAGTTTTGGAGACCCAGGAGCTATGCTCT CTCGCCCTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTAGAGTCCGTGACAGCT ATGGATCCCACAAAAGTTGGAGGCCGGTCTCAGTACCAGCACCTTCTGCGACTTGAGGAT GGGACTGATATCGTGAAGTGCAGAACTGAGTGGCGGCCGAAGAATCCAGGAGCTAATGG

GGCAATATCAACGGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG

SEQ ID NO: 90

Cuphea avigera var. pulcherrima (Ca) FATB1 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCTCCGTGCCCGTGCCCGGCACCTCCCCCA AGCCCGGCAAGTTCCGCATCTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGCCCAAGCCCAT CCCCAACGGCGGCCTGCAGGTGAAGGCCAACTCCCGCGCCCACCCCAAGGCCAACGGCTC CGCCGTGTCCCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCC CCCCGCACCTTCCTGCACCAGCTGCCCGACTGGTCCCGCCTGCTGACCGCCATCACCACCG

TGTTCGTGAAGTCCAAGCGCCCCGACATGCACGACCGCAAGTCCAAGCGCCCCGACATGC

TGATGGACTCCTTCGGCCTGGAGTCCATCGTGCAGGAGGGCCTGGAGTTCCGCCAGTCCTT CTCCATCCGCTCCTACGAGATCGGCACCGACCGCACCGCCTCCATCGAGACCCTGATGAA

CTACCTGCAGGAGACCTCCCTGAACCACTGCAAGTCCACCGGCATCCTGCTGGACGGCTTC GGCCGCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGATGAAGATC

0 AAGGTGAACCGCTACCCCGCCTGGGGCGACACCGTGGAGATC AACACCTGGTTCTCCCGC

CTGGGCAAGATCGGCAAGGGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGATC CTGATCCGCGCCACCTCCGCCTACGCCACCATGAACCAGAAGACCCGCCGCCTGTCCAAG

CTGCCCTACGAGGTGCACCAGGAGATCGCCCCCCTGTTCGTGGACTCCCCCCCCGTGATCG AGGACAACGACCTGAAGCTGCACAAGTTCGAGGTGAAGACCGGCGACTCCATCCACAAG

5 GGCCTGACCCCCGGCTGGAACGACCTGGACGTGAACC AGCACGTGTCC AACGTGAAGT AC

ATCGGCTGGATCCTGGAGTCCATGCCCACCGAGGTGCTGGAGACCCAGGAGCTGTGCTCC CTGGCCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCC

ATGGACCCCACCAAGGTGGGCGGCCGCTCCCAGTACCAGCACCTGCTGCGCCTGGAGGAC GGCACCGACATCGTGAAGTGCCGCACCGAGTGGCGCCCCAAGAACCCCGGCGCCAACGG

CGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCC

SEQ ID NO: 91

Cuphea paucipetala (Cpau) FATB1 amino acid sequence

MVAAAASSAFFPVPAPGTSPKPGKSGNWPSSLSPSIKPMSIPNGGFQVKANASAHPKANGSAV

NLKSGSLNTQEDTS SSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMRDRKSKRPDMLVD

SVGLKSWLDGLVSRQIFSIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMC KNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSHSGKIGMASDWLITDCNTGEILIRATSVWA MMNQKTRRFSRLPYEVRQELTPHYVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLDV NQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAMDPSEDEGRSQYKH

0 LLRLEDGTDIVKGRTEWRPKNAGTNGAISTAKPSNGNSVS

SEQ ID NO: 92

Cuphea paucipetala (Cpau) FATB1 coding DNA sequence

ATGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGTTCCAGCCCCCGGAACCTCCCCTA

5 AACCCGGGAAGTCCGGC AACTGGCCATC AAGCTTGAGCCCTTCC ATCAAGCCC ATGTC AA

2018267601 21 Nov 2018

TCCCCAATGGCGGATTTCAGGTTAAGGCAAATGCCAGTGCCCATCCTAAGGCTAACGGTT

CTGCAGTAAATCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCCTCC TCCTCGGGCTTTCCTTAACCAGTTGCCTGATTGGAGTATGCTTCTGACTGCAATCACGACC

GTCTTCGTGGCGGCAGAGAAGCAGTGGACTATGCGTGATCGGAAATCTAAGAGGCCTGAC

ATGCTCGTGGACTCGGTTGGGTTGAAGAGTGTTGTTCTGGATGGGCTCGTGTCCAGACAGA _{TTTTTTCGATTAGGTCTTATGAAATAGGCGCTGATCGAACTGCCTCTATAGAGACGCTGAT} GAACCACTTGCAGGAAACATCTATCAATCATTGTAAGAGTTTGGGTCTTCTCAATGACGGC TTTGGTCGTACTCCTGGGATGTGTAAAAATGACCTCATTTGGGTGCTTACAAAAATGCAGA TCATGGTGAATCGCTACCCAACTTGGGGCGATACTGTTGAGATCAATACCTGGTTCTCCCA

TTCGGGGAAAATTGGTATGGCTAGCGATTGGCTAATAACTGATTGCAACACAGGAGAAAT

TCTTATAAGAGCAACGAGCGTGTGGGCCATGATGAATCAAAAGACGAGAAGATTCTCAAG ACTTCCATACGAGGTTCGCCAGGAGTTAACGCCTCATTATGTGGACTCTCCTCATGTCATT

GAAGATAATGATCGGAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTCGTAAG GGTCTAACTCCGAGGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTAC

ATTGGGTGGATTCTCGAGAGTATGCCAATAGAAGTTTTGGAGACCCAGGAGCTATGCTCT

CTCACCGTTGAATATAGGCGGGAATGCGGAATGGACAGTGTGCTGGAGTCCGTGACTGCT ATGGATCCCTCAGAAGATGAAGGCCGGTCTCAGTACAAGCACCTTCTGCGGCTTGAGGAT

GGGACTGACATCGTGAAGGGCAGAACTGAGTGGCGACCGAAGAATGCAGGAACTAACGG GGCGATATCAACAGCAAAGCCTTCAAATGGAAACTCGGTCTCTTAG

SEQ ID NO: 93

Cuphea paucipetala (Cpau) FATB1 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCTCCCCCA AGCCCGGCAAGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCATCAAGCCCATGTCCAT

CCCCAACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC

CGCCGTGAACCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCC CCCCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGACCGCCATCACCACCG

TGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCGCGACCGCAAGTCCAAGCGCCCCGACA TGCTGGTGGACTCCGTGGGCCTGAAGTCCGTGGTGCTGGACGGCCTGGTGTCCCGCCAGA

TCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGAT

GAACCACCTGCAGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGG CTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCA GATCATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACACCTGGTTCTC

CCACTCCGGCAAGATCGGCATGGCCTCCGACTGGCTGATCACCGACTGCAACACCGGCGA

GATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCTCC

CGCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTACGTGGACTCCCCCCACGTG

ATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCCGC AAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAG

TACATCGGCTGGATCCTGGAGTCCATGCCCATCGAGGTGCTGGAGACCCAGGAGCTGTGC

TCCCTGACCGTGGAGTACCGCCGCGAGTGCGGCATGGACTCCGTGCTGGAGTCCGTGACC

GCCATGGACCCCTCCGAGGACGAGGGCCGCTCCCAGTACAAGCACCTGCTGCGCCTGGAG GACGGCACCGACATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCACCAA CGGCGCCATCTCCACCGCCAAGCCCTCCAACGGCAACTCCGTGTCCTGA

SEQ ID NO: 94

Cuphea procumbens (Cproc) FATB1 amino acid sequence

MVAAAASSAFFPAPAPGSSPKPGKSGNWPSSLSPSFKSKSIPYGRFQVKANASAHPKANGSAV NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVD SVGLKNIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGM

CKNDLIWVLTKMQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVW AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLD VNQHVSNVKYIGWILESMPIEVLEAQELCSLTVEYRRECGMDSVLESVTAVDPSEDGGRSQYN HLLRLEDGTDWKGRTEWRPKNAETNGAISPGNTSNGNSIS

2018267601 21 Nov 2018

SEQ ID NO: 95

Cuphea procumbens (Cproc) FATB1 coding DNA sequence

ATGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGCTCCAGCCCCGGGATCCTCACCTA

AACCCGGGAAGTCCGGTAATTGGCCATCGAGCTTGAGCCCTTCCTTCAAGTCCAAGTCAAT

CCCCTATGGCCGATTTCAGGTTAAGGCAAATGCCAGTGCCCATCCTAAGGCTAACGGTTCT

GCAGTAAATCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCCTCCTC CTCGGGCTTTCCTTAACCAGTTGCCTGATTGGAGTATGCTTCTGTCTGCAATCACGACTGT

ATTCGTGGCGGCAGAGAAGCAGTGGACTATGCTTGATCGGAAATCTAAGAGGCCTGACAT

GCTTGTGGACTCGGTTGGGTTGAAGAATATTGTTCGGGATGGGCTCGTGTCCAGACAGAG

TTTTTTGATTAGATCTTATGAAATAGGCGCTGATCGAACAGCTTCTATAGAGACACTGATG

AACCACTTGCAGGAAACATCTATCAATCATTGTAAGAGTTTGGGTCTTCTCAATGACGGCT

TTGGTCGTACTCCTGGGATGTGTAAAAACGACCTCATTTGGGTGCTTACTAAAATGCAGAT

CATGGTGAATCGCTACCCAGCTTGGGGCGATACTGTTGAGATCAATACCTGGTTCTCCCAG

TCGGGGAAAATCGGTATGGGTAGCGATTGGCTAATAAGTGATTGCAACACAGGAGAAATT 15 CTTATAAGAGCAACGAGCGTGTGGGCCATGATGAATCAAAAAACGAGAAGATTCTCAAG

ACTTCCATACGAGGTTCGCCAGGAGTTAACGCCTCATTTTGTGGACTCTCCTCATGTCATT

GAAGACAATGATCGGAAATTGCATAAGTTCGATGTGAAGACTGGTGATTCTATTCGCAAG

GGTCTAACTCCGAGGTGGAATGACTTGGATGTCAATCAGCACGTGAGCAACGTGAAGTAC

ATTGGGTGGATTCTCGAGAGTATGCCAATAGAAGTTTTGGAGGCCCAGGAACTATGCTCT

CTCACCGTTGAATATAGGCGGGAATGCGGAATGGACAGTGTGCTGGAGTCCGTGACTGCT

GTAGATCCCTCAGAAGATGGAGGCCGGTCTCAGTACAATCACCTTCTGCGGCTTGAGGAT GGGACTGATGTCGTGAAGGGCAGAACTGAGTGGCGACCGAAGAATGCAGAAACTAACGG GGCGATATCACCAGGAAACACTTCAAATGGAAACTCGATCTCCTAG

SEQ ID NO: 96

Cuphea procumbens (Cproc) FATB1 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGCCCCCGCCCCCGGCTCCTCCCCCA

AGCCCGGCAAGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGTCCAAGTCCAT 3 0 CCCCTACGGCCGCTTCCAGGTGAAGGCC AACGCCTCCGCCC ACCCC AAGGCC AACGGCTC

CGCCGTGAACCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCC

CCCCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGTCCGCCATCACCACCG

TGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCGACA

TGCTGGTGGACTCCGTGGGCCTGAAGAACATCGTGCGCGACGGCCTGGTGTCCCGCCAGT

CCTTCCTGATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGAT

GAACCACCTGCAGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGG CTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCA GATCATGGTGAACCGCTACCCCGCCTGGGGCGACACCGTGGAGATCAACACCTGGTTCTC

CCAGTCCGGCAAGATCGGCATGGGCTCCGACTGGCTGATCTCCGACTGCAACACCGGCGA

GATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCTCC

CGCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTTCGTGGACTCCCCCCACGTG

ATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCCGC

AAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAG

TACATCGGCTGGATCCTGGAGTCCATGCCCATCGAGGTGCTGGAGGCCCAGGAGCTGTGC 45 TCCCTGACCGTGGAGTACCGCCGCGAGTGCGGCATGGACTCCGTGCTGGAGTCCGTGACC

GCCGTGGACCCCTCCGAGGACGGCGGCCGCTCCCAGTACAACCACCTGCTGCGCCTGGAG GACGGCACCGACGTGGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGAGACCAA CGGCGCCATCTCCCCCGGCAACACCTCCAACGGCAACTCCATCTCCTGA

SEQ ID NO: 97

Cuphea procumbens (Cproc) FATB2 amino acid sequence

MVAAAASSAFFPAPAPGSSPKPGKSGNWPSSLSPSFKSKSIPYGRFQVKANASAHPKANGSAV

NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVD

SVGLKNIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGM

CKNDLIWVLTKMQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVW

2018267601 21 Nov 2018

AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLD

VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRQECGRESVLESLTAVDPSGKGFGSQFQH LLRLEDGGEIVKGRTEWRPKTAGINGAIASGETSPGDF

SEQ ID NO: 98

Cuphea procumbens (Cproc) FATB2 coding DNA sequence

ATGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGCTCCAGCCCCGGGATCCTCACCTA

AACCCGGGAAGTCCGGTAATTGGCCATCGAGCTTGAGCCCTTCCTTCAAGTCCAAGTCAAT

CCCCTATGGCCGATTTCAGGTTAAGGCAAATGCCAGTGCCCATCCTAAGGCTAACGGTTCT 10 GCAGTAAATCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCCTCCTC

CTCGGGCTTTCCTTAACCAGTTGCCTGATTGGAGTATGCTTCTGTCTGCAATCACGACTGT

ATTCGTGGCGGCAGAGAAGCAGTGGACTATGCTTGATCGGAAATCTAAGAGGCCTGACAT

GCTTGTGGACTCGGTTGGGTTGAAGAATATTGTTCGGGATGGGCTCGTGTCCAGACAGAG _{TTTTTTGATTAGATCTTATGAAATAGGCGCTGATCGAACAGCTTCTATAGAGACACTGATG} 15 AACCACTTGCAGGAAACATCTATCAATCATTGTAAGAGTTTGGGTCTTCTCAATGACGGCT

TTGGTCGTACTCCTGGGATGTGTAAAAACGACCTCATTTGGGTGCTTACTAAAATGCAGAT

CATGGTGAATCGCTACCCAGCTTGGGGCGATACTGTTGAGATCAATACCTGGTTCTCCCAG

TCGGGGAAAATCGGTATGGGTAGCGATTGGCTAATAAGTGATTGCAACACAGGAGAAATT

CTTATAAGAGCAACGAGCGTGTGGGCCATGATGAATCAAAAAACGAGAAGATTCTCAAG

ACTTCCATACGAGGTTCGCCAGGAGTTAACGCCTCATTTTGTGGACTCTCCTCATGTCATT

GAAGACAATGATCGGAAATTGCATAAGTTCGATGTGAAGACTGGTGATTCTATTCGCAAG

GGTCTAACTCCGAGGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTAC

ATCGGGTGGATTCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGTTCCC

TTACCCTGGAATACAGGCAGGAATGCGGAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTG

TGGACCCCTCTGGAAAGGGCTTTGGGTCCCAGTTCCAACACCTTCTGAGGCTTGAGGATGG

AGGTGAGATCGTGAAGGGGAGAACTGAGTGGCGACCCAAGACTGCAGGTATCAATGGGG

CGATAGCATCCGGGGAGACCTCACCTGGAGACTTTTAG

SEQ ID NO: 99

Cuphea procumbens (Cproc) FATB2 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGCCCCCGCCCCCGGCTCCTCCCCCA

AGCCCGGCAAGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGTCCAAGTCCAT

CCCCTACGGCCGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC 3 5 CGCCGTGAACCTGAAGTCCGGCTCCCTGAAC ACCCAGGAGGAC ACCTCCTCCTCCCCCCCC

CCCCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGTCCGCCATCACCACCG

TGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCGACA

TGCTGGTGGACTCCGTGGGCCTGAAGAACATCGTGCGCGACGGCCTGGTGTCCCGCCAGT

CCTTCCTGATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGAT

GAACCACCTGCAGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGG

CTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCA

GATCATGGTGAACCGCTACCCCGCCTGGGGCGACACCGTGGAGATCAACACCTGGTTCTC

CCAGTCCGGCAAGATCGGCATGGGCTCCGACTGGCTGATCTCCGACTGCAACACCGGCGA

GATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCTCC 45 CGCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTTCGTGGACTCCCCCCACGTG

ATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCCGC

AAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAA

GTACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTG

CTCCCTGACCCTGGAGTACCGCCAGGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGAC 5 0 CGCCGTGGACCCCTCCGGC AAGGGCTTCGGCTCCC AGTTCC AGC ACCTGCTGCGCCTGGA

GGACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGACCGCCGGCATCA

ACGGCGCCATCGCCTCCGGCGAGACCTCCCCCGGCGACTTCTGA

SEQ ID NO: 100

Cuphea procumbens (Cproc) FATB3 amino acid sequence

2018267601 21 Nov 2018

MVAAAASSAFFPAPAPGSSPKPGKSGNWPSSLSPSFKSKSIPYGRFQVKANASAHPKANGSAV

NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVD SVGLKNIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGM

CKNDLIWVLTKMQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVW

AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLD

VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGEGGYGSQFQ HLLRLEDGGEIVKGRTEWRPKNAGINGVLPTGE*

SEQ ID NO: 101

Cuphea procumbens (Cproc) FATB3 coding DNA sequence

ATGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGCTCCAGCCCCGGGATCCTCACCTA

AACCCGGGAAGTCCGGTAATTGGCCATCGAGCTTGAGCCCTTCCTTCAAGTCCAAGTCAAT

CCCCTATGGCCGATTTCAGGTTAAGGCAAATGCCAGTGCCCATCCTAAGGCTAACGGTTCT

GCAGTAAATCTAAAGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCCTCCTC 15 CTCGGGCTTTCCTTAACCAGTTGCCTGATTGGAGTATGCTTCTGTCTGCAATCACGACTGT

ATTCGTGGCGGCAGAGAAGCAGTGGACTATGCTTGATCGGAAATCTAAGAGGCCTGACAT

GCTTGTGGACTCGGTTGGGTTGAAGAATATTGTTCGGGATGGGCTCGTGTCCAGACAGAG _{TTTTTTGATTAGATCTTATGAAATAGGCGCTGATCGAACAGCTTCTATAGAGACACTGATG}

AACCACTTGCAGGAAACATCTATCAATCATTGTAAGAGTTTGGGTCTTCTCAATGACGGCT 20 TTGGTCGTACTCCTGGGATGTGTAAAAACGACCTCATTTGGGTGCTTACTAAAATGCAGAT

CATGGTGAATCGCTACCCAGCTTGGGGCGATACTGTTGAGATCAATACCTGGTTCTCCCAG

TCGGGGAAAATCGGTATGGGTAGCGATTGGCTAATAAGTGATTGCAACACAGGAGAAATT

CTTATAAGAGCAACGAGCGTGTGGGCCATGATGAATCAAAAAACGAGAAGATTCTCAAG

ACTTCCATACGAGGTTCGCCAGGAGTTAACGCCTCATTTTGTGGACTCTCCTCATGTCATT 25 GAAGACAATGATCGGAAATTGCATAAGTTCGATGTGAAGACTGGTGATTCTATTCGCAAG

GGTCTAACTCCGAGGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTAC

ATCGGGTGGATTCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGTTCCC

TTACCCTGGAATACAGGCGGGAATGTGGAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTG

TGGACCCCTCTGGAGAGGGGGGCTATGGATCCCAGTTTCAGCACCTTCTGCGGCTTGAGG

0 ATGGAGGTGAGATCGTGAAGGGGAGAACTGAGTGGCGACCC AAGAATGCTGGAATCAAT

GGGGTGTTACCAACCGGGGAGTAG

SEQ ID NO: 102

Cuphea procumbens (Cproc) FATB3 coding DNA sequence codon optimized for Prototheca moriformis

ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGCCCCCGCCCCCGGCTCCTCCCCCA

AGCCCGGCAAGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGTCCAAGTCCAT

CCCCTACGGCCGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC

CGCCGTGAACCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCC 40 CCCCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGTCCGCCATCACCACCG

TGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCGACA

TGCTGGTGGACTCCGTGGGCCTGAAGAACATCGTGCGCGACGGCCTGGTGTCCCGCCAGT

CCTTCCTGATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGAT

GAACCACCTGCAGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGG 45 CTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCA

GATCATGGTGAACCGCTACCCCGCCTGGGGCGACACCGTGGAGATCAACACCTGGTTCTC

CCAGTCCGGCAAGATCGGCATGGGCTCCGACTGGCTGATCTCCGACTGCAACACCGGCGA

GATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCTCC

CGCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTTCGTGGACTCCCCCCACGTG

0 ATCGAGGAC AACGACCGC AAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCC ATCCGC

AAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAA

GTACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTG

CTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGAC

CGCCGTGGACCCCTCCGGCGAGGGCGGCTACGGCTCCCAGTTCCAGCACCTGCTGCGCCT

5 GGAGGACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCC AAGAACGCCGGC A

TCAACGGCGTGCTGCCCACCGGCGAGTGA

2018267601 21 Nov 2018

SEQ ID NO: 103

Cuphea ignea (Cignea) FATB1 amino acid sequence

PGTSRKTGKFGNWPSSLSPSFKPKSIPNGGFQVKANARAHPKANGSAVSLKSVSLNTQEDTSLS PPPRAFLNQLPDWRMLRTALTTVFVAAEKQWTMLDRKSKRPDMLVDSFGLESIVQEGLVFRQ

SFSIRSYEIGIDRTASIETLMNHLQETSLNQCKSAGILHDGFGRTLEMCKRDLIWWTKMQIKV

NRYPAWGDTVEISTRFSRLGKIGMGRDWLICDCNTGEILIRATSAYAMMNQKTRRLSKLPNEV RQEIAPLFVDSDPVIEENDMKLHKFEVKTGDSICKGLTPRWSDLDVNQHVSNVKYIGWILESM PTEVLETQELCSLALEYRRECGRDSVLESVTSMDPSKVGGWSQYQHLLRLEDGADIVKGRTE WRPKNAGANGAISTGKT

SEQ ID NO: 104

Cuphea ignea (Cignea) FATB1 coding DNA sequence

CCGGGAACCTCACGTAAAACCGGGAAGTTCGGCAATTGGCCATCAAGCTTGAGCCCTTCC

TTCAAGCCCAAGTCAATCCCCAATGGCGGATTTCAGGTTAAGGCTAATGCCAGAGCCCAT

CCTAAGGCTAACGGTTCTGCAGTAAGTCTAAAGTCTGTCAGCCTCAACACTCAGGAGGAC

ACTTCGTTGTCCCCTCCTCCTCGTGCTTTCCTTAACCAGTTGCCTGATTGGAGGATGCTTCG GACTGCACTCACGACCGTCTTTGTGGCGGCAGAGAAGCAGTGGACTATGCTTGATCGGAA ATCTAAGAGGCCTGACATGCTCGTGGACTCGTTTGGGTTGGAGAGTATTGTTCAAGAAGG

GCTCGTGTTCAGACAGAGCTTTTCGATTAGGTCTTATGAAATAGGCATTGATCGAACAGCC

TCTATAGAGACGCTGATGAACCACTTGCAGGAAACATCTCTCAATCAATGTAAGAGTGCT

GGTATTCTCCATGACGGCTTCGGTCGTACTCTTGAGATGTGTAAAAGGGACCTCATTTGGG TTGTTACGAAAATGCAGATCAAGGTGAATCGCTATCCAGCTTGGGGCGATACTGTCGAGA

TCAGTACCCGGTTCTCCCGGTTGGGGAAAATCGGTATGGGTCGCGATTGGCTAATATGTGA TTGCAACACAGGAGAAATTCTTATAAGAGCAACGAGCGCGTATGCCATGATGAATCAAAA

GACGAGAAGACTCTCAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCTTTTTGT

GGACTCTGATCCTGTCATTGAAGAAAATGATATGAAATTGCATAAGTTTGAAGTGAAGAC

TGGTGATTCCATTTGCAAGGGTCTAACTCCGAGGTGGAGTGACTTGGATGTCAATCAGCAC

GTAAGCAACGTGAAGTACATAGGGTGGATTCTCGAGAGTATGCCAACAGAAGTTTTGGAG

ACACAGGAGCTATGCTCTCTCGCCCTTGAATATAGGCGGGAATGCGGAAGGGACAGTGTG

CTGGAGTCTGTGACCTCTATGGATCCCTCAAAAGTTGGAGGCTGGTCTCAGTACCAGCACC

TTCTGCGACTTGAGGATGGGGCGGATATCGTGAAGGGCAGAACTGAGTGGCGGCCGAAG AATGCAGGAGCTAACGGGGCGATATCAACAGGAAAGACTTGA

SEQ ID NO: 105

Cuphea ignea (Cignea) FATB1 coding DNA sequence codon optimized for Prototheca moriformis

CCCGGCACCTCCCGCAAGACCGGCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCT

TCAAGCCCAAGTCCATCCCCAACGGCGGCTTCCAGGTGAAGGCCAACGCCCGCGCCCACC

CCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGTCCGTGTCCCTGAACACCCAGGAGGACA

CCTCCCTGTCCCCCCCCCCCCGCGCCTTCCTGAACCAGCTGCCCGACTGGCGCATGCTGCG

CACCGCCCTGACCACCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAA

GTCCAAGCGCCCCGACATGCTGGTGGACTCCTTCGGCCTGGAGTCCATCGTGCAGGAGGG

CCTGGTGTTCCGCCAGTCCTTCTCCATCCGCTCCTACGAGATCGGCATCGACCGCACCGCC

TCCATCGAGACCCTGATGAACCACCTGCAGGAGACCTCCCTGAACCAGTGCAAGTCCGCC

GGCATCCTGCACGACGGCTTCGGCCGCACCCTGGAGATGTGCAAGCGCGACCTGATCTGG

GTGGTGACCAAGATGCAGATCAAGGTGAACCGCTACCCCGCCTGGGGCGACACCGTGGAG

ATCTCCACCCGCTTCTCCCGCCTGGGCAAGATCGGCATGGGCCGCGACTGGCTGATCTGCG ACTGCAACACCGGCGAGATCCTGATCCGCGCCACCTCCGCCTACGCCATGATGAACCAGA

AGACCCGCCGCCTGTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCTGTTCG TGGACTCCGACCCCGTGATCGAGGAGAACGACATGAAGCTGCACAAGTTCGAGGTGAAG

0 ACCGGCGACTCC ATCTGC AAGGGCCTGACCCCCCGCTGGTCCGACCTGGACGTGAACC AG

CACGTGTCCAACGTGAAGTACATCGGCTGGATCCTGGAGTCCATGCCCACCGAGGTGCTG

GAGACCCAGGAGCTGTGCTCCCTGGCCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCC

GTGCTGGAGTCCGTGACCTCCATGGACCCCTCCAAGGTGGGCGGCTGGTCCCAGTACCAG

CACCTGCTGCGCCTGGAGGACGGCGCCGACATCGTGAAGGGCCGCACCGAGTGGCGCCCC

5 AAGAACGCCGGCGCC AACGGCGCC ATCTCC ACCGGC AAGACCTGA

2018267601 21 Nov 2018

SEQ ID NO: 106

JcFatBl consensus amino acid sequence

MVAAAASSAFFPVPAPGTSPKPGKSGNWPSSLSPSFKPKSIPNGGFQVKANASAHPKANGSAV NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRPDMLVD

SVGLKRIVQDGLVSRQSFSIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGM CKNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVW AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLD VNQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAMDPSENGGRSQYK HLLRLEDGTDIVKGRTEWRPKNAGTNGAISTGKTSNGNSVS*

SEQ ID NO: 107

JcFatBl consensus DNA sequence codon optimized for Prototheca

ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCTCCCCCA AGCCCGGCAAGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGCCCAAGTCCAT

CCCCAACGGCGGCTTCCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTC CGCCGTGAACCTGAAGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCC CCCCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGACCGCCATCACCACCG TGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTCCAAGCGCCCCGACA TGCTGGTGGACTCCGTGGGCCTGAAGCGCATCGTGCAGGACGGCCTGGTGTCCCGCCAGT

CCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGAT GAACCACCTGCAGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGG CTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCA GATCATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGATCAACACCTGGTTCTC CCAGTCCGGCAAGATCGGCATGGGCTCCGACTGGCTGATCTCCGACTGCAACACCGGCGA

GATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCTCC CGCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTTCGTGGACTCCCCCCACGTG ATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCCGC AAGGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAG TACATCGGCTGGATCCTGGAGTCCATGCCCATCGAGGTGCTGGAGACCCAGGAGCTGTGC

TCCCTGACCGTGGAGTACCGCCGCGAGTGCGGCATGGACTCCGTGCTGGAGTCCGTGACC GCCATGGACCCCTCCGAGAACGGCGGCCGCTCCCAGTACAAGCACCTGCTGCGCCTGGAG GACGGCACCGACATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCACCAA CGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA

SEQ ID NO: 108

JcFatB2 consensus amino acid sequence

MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLSPLKPKSVANGGLQVKANASAPPKINGSSVGL KSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPF GLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFGRTPEMY

KRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSV WVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTPKWNDLD VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKGYGSQFQ HLLRLEDGGEIVKGRTEWRPKTAGINGAIASGETSPGDSS*

SEQ ID NO: 109

JcFatB2 consensus DNA sequence codon optimized for Prototheca

ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCCCCGACACCTCCTCCCG CCCCGGCAAGCTGGGCAACGGCTCCTCCTCCCTGTCCCCCCTGAAGCCCAAGTCCGTGGCC AACGGCGGCCTGCAGGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCC

0 GTGGGCCTGAAGTCCGGCTCCCTGAAGACCCAGGAGGACACCCCCTCCGCCCCCCCCCCC

CGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGCTGCTGGCCGCCATCACCACCGTGT TCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAAGCGCCCCGACATGC

TGGTGGACCCCTTCGGCCTGGGCCGCATCGTGCAGGACGGCCTGGTGTTCCGCCAGAACTT

2018267601 21 Nov 2018

CTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAA

CCACCTGCAGGAGACCGCCCTGAACCACGTGAAGTCCGCCGGCCTGCTGAACGACGGCTT CGGCCGCACCCCCGAGATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGT GATGGTGAACCGCTACCCCACCTGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCCAA

GTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGATCTCCGACTGCAACACCGGCGAGAT

CCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGACCCGCCGCCTGTCCAA

GATCCCCGACGAGGTGCGCCACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCCGTGAT

CGAGGACGACGACCGCAAGCTGCCCAAGCTGGACGAGAAGACCGCCGACTCCATCCGCA AGGGCCTGACCCCCAAGTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAG 10 TACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGC

TCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACC GCCGTGGACCCCTCCGGCAAGGGCTACGGCTCCCAGTTCCAGCACCTGCTGCGCCTGGAG GACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGACCGCCGGCATCAA CGGCGCCATCGCCTCCGGCGAGACCTCCCCCGGCGACTCCTCCTGA

SEQ ID NO: 110

CuPSR23 FATB3 amino acid sequence

MWAAATSAFFPVPAPGTSPKPGKSGNWPSSLSPTFKPKSIPNAGFQVKANASAHPKA

NGSAVNLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRP

DMLVDSVGLKCIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFG

RTPGMCKNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSQSGKIGMASDWLISDCNTGEILIR ATSVWAMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDQKLHKFDVKTGDSIRKGLTPR WNDLDVNQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAVDPSENG GRSQYKHLLRLEDGTDIVKSRTEWRPKNAGTNGAISTSTAKTSNGNSVS

SEQ ID NO: 111

CuPSR23 FATB3b amino acid sequence

MWAAATSAFFPVPAPGTSPKPGKSGNWPSSLSPTFKPKSIPNAGFQVKANASAHPKA NGSAVNLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRP DMLVDSVGLKSIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFG 30 RTPGMCKNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSQSGKIGMASDWLISDCNTGEILIR ATSVWAMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDQKLHKFDVKTGDSIRKGLTPR WNDLDVNQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAVDPSENG GRSQYKHLLRLEDGTDIVKSRTEWRPKNAGTNGAISTSTAKTSNGNSAS

SEO ID NO: 112

CwFATB3 amino acid sequence:

MWAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSPHPK

ANGSAVSLKSGSLNTLEDPPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQFTRLDRKSKRPD

MLVDWFGSETIVQDGLVFRERFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFG RTSEMCTRDLIWVLTKMQIWNRYPTWGDTVEINSWFSQSGKIGMGRDWLISDCNTGEILVR 40 ATSAWAMMNQKTRRFSKLPCEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPG

WNDLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRRECGRESWESVTSMNPSKVG DRSQYQHLLRLEDGADIMKGRTEWRPKNAGTNRAIST

SEO ID NO: 113

CwFATB3a amino acid sequence:

MWAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSPHPK

ANGSAVSLKSGSLNTLEDPPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQFTRLDRKSKRPD MLVDWFGSETIVQDGLVFRERFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFG RTSEMCTRDLIWVLTKMQIWNRYPTWGDTVEINSWFSQSGKIGMGRDWLISDCNTGEILVR ATSAWAMMNQKTRRFSKLPCEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPG 50 WNDLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRRECGRESWESVTSMNPSKVG

DRSQYQHLLRLEDGADIMKGRTEWRPKNAGTNRAIST

2018267601 21 Nov 2018

SEP ID NO: 114

CwFATB3b amino acid sequence

MWAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSPHPK

ANGSAVSLKSGSLNTLEDLPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQFTRLDRKSKRPD

MLVDWFGSETIVQDGLVFRERFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFG

RTSEMCTRDLIWVLTKMQIWNRYPTWGDTVEINSWFSQSGKIGMGRDWLISDCNTGEILVR ATSAWAMMNQKTRRFSKLPCEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPG WNDLDVNQHVSNVKYIGWILEKFWRPRSYALSPLNIGGNVEGKVW

SEO ID NO: 115

CwFATB3c amino acid sequence

MWAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSPHPK

ANGSAVSLKSGSLNTLEDLPSSPPPRTFLNQLPDWSRLRTAITTVFVATEKQFTRLDRKSKRPD

MLVDWFGSETIVQDGLVFRERFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFG RTSEMCTRDLIWVLTKMQIWNRYPTWGDTVEINSWFSQSGKIGMGRDWLISDCNTGEILVR 15 ATSAWAMMNQKTRRFSKLPCEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPG

WNDLDVNQHVSNVKYIGWILEKFWRPRSYALSPLNIGGNVEGKVW

SEO ID NO: 116

CwFATB4a amino acid sequence

MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPPKIN

GSSVGLKSGGFKTQEDSPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD

MLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSNDGFGR

TPEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILT

RASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPWEDDDRKLPKLDENTADSIRKGLTPR WNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSAEGY 25 ASRFQHLLRLEDGGEIVKARTEWRPKNAGINGWPSEESSPGDFF

SEO ID NO: 117

CwFATB4a.l amino acid sequence

MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPPKIN

GSSVGLKSGGFKTQEDSPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD

MLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSNDGFGR

TPEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILT

RASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPWEDDDRKLPKLDENTADSIRKGLTPR WNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSAEGY ASRFQHLLRLEDGGEIVKARTEWRPKNAGINWWPSEESSPGDFF

SEO ID NO: 118

CwFATB4a,2 amino acid sequence:

MVATAASSAFFPVPSADTSSSRPGKLGNGPSSLSPLKPKSIPNGGLQVKANASAPPKIN GSSVGLKSGSFKTQEDAPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSNDGFGR 40 TPEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILT RASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPWEDDDRKLPKLDENTADSIRKGLTPR WNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSAEGY ASRFQHLLRLEDGGEIVKARTEWRPKNAGINGWPSEESSPGDFF

SEO ID NO: 119

CwFATB4a,3 amino acid sequence

MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPPKIN

GSSVGLKSGGFKTQEDSPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD

MLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSNDGFGR

TPEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILT

RASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPWEDDDRKLPKLDENTADSIRKGLTPR

2018267601 21 Nov 2018

WNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSAEGY

VSRFQHLLRLEDGGEIVKARTEWRPKNAGINGWPSEESSPGDFF

SEO ID NO: 120

CwFATB4b amino acid sequence

MVATAASSAFFPVPSADTSSSRPGKLGNGPSSLSPLKPKSIPNGGLQVKANASAPPKIN

GSSVGLKSGSFKTQEDAPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSSDGFGR TPAMSKRDLIWWAKMQVMVNRYPAWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILT RASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPWEDDDRKLPKLDENTADSIRKGLTPR 10 WNDLDVNQHVNNVKYIGWILESTPAEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGEGD GSKFQHLLRLEDGGEIVKARTEWRPKNAGINGWPSEESSPGGDFF

SEO ID NO: 121

CwFATB4b. 1 amino acid sequence

MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPPKIN

GSSVGLKSGSFKTQEDAPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD

MLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSSDGFGR TPAMSKRDLIWWAKMQVMVNRYPAWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILT RASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPWEDDDRKLPKLDENTADSIRKGLTPR WNDLDVNQHVNNVKYIGWILESTPAEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGEGD 20 GSKFQHLLRLEDGGEIVKARTEWRPKNAGINGWPSEESSPGGDFF

SEO ID NO: 122

CwFATB5 amino acid sequence

MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPKANG

SAVNLKSGSLETPPRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRIFQDGVFF

RQSFSIRSYEIGVDRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWWTKIQVE

VNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNQNTRRLSKFP YEVRQEIAPHFVDSAPVIEDDQKLQKLDVKTGDSIRDGLTPRWNDLDVNQHVNNVKYIGWIL KSVPIEVFETQELCGVTLEYRRECGRDSVLESVTAMDPAKEGDRCVYQHLLRLEDGADITIGR TEWRPKNAGANGAMSSGKTSNGNCLIEGRGWQPFRWRLIF

SEO ID NO: 123

CwFATB5a amino acid sequence

MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPKANG

SAVNLKSGSLETPPRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRIFQDGFFFR QSFSIRSYEIGVDRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWWTKIQVEV 35 NRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNQNTRRLSKFPYE

VRQEIAPHFVDSAPVIEDDQKLQKLDVKTGDSIRDGLTPRWNDLDVNQHVNNVKYIGWILKS VPIEVFETQELCGVTLEYRRECGRDSVLESVTAMDPAKEGDRCVYQHLLRLEDGADITIGRTE WRPKNAGANGAMSSGKTSNGNCLIEGRGWQPFRWRLIF

SEO ID NO: 124

CwFATB5b amino acid sequence

MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPKANG

SAVNLKSGSLETPPRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRIFQDGVFF

RQSFSIRSYEIGVDRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWWTKIQVE VNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNQNTRRLSKFP 45 YEVRQEIAPHFVDSAPVIEDDQKLQKLDVKTGDSIRDGLTPRWNDLDVNQHVNNVKYIGWIL

KSVPIEVFETQELCGVTLEYRRECGRDSVLESVTAMDPAKEGDRCVYQHLLWLEDGADITIGR TEWRPKNAGANGAMSSGKTSNGNCLIEGRGWQPFRWRLIF

SEO ID NO: 125

CwFATB5c amino acid sequence

MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPKANG

2018267601 21 Nov 2018

SAVNLKSGSLETPPRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRIFQDGVFF

RQSFSIRSYEIGVDRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWWTKIQVE VNRYPIWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNQNTRRLSKFPY EVRQEIAPHFVDSAPVIEDDQKLQKLDVKTGDSIRDGLTPRWNDLDVNQHVNNVKYIGWILK

SVPIEVFETQELCGVTLEYRRECGRDSVLESVTAMDPAKEGDRCVYQHLLRLEDGADITIGRTE WRPKNAGANGAMSSGKTSNGNCLIEGMGWQPFRWRLIF

SEO ID NO: 126

CwFATB5.1 amino acid sequence

MVAAAASSAFFSVPTPGTSPKPGKFRNWPSSLSVPFKPETNHNGGFHIKANASAHPKA

NGSALNLKSGSLETQEDTSLSSPPRTFIKQLPDWSMLLSKITTVFGAAEKQLKRPGMLVEPFGV

DRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLI WWTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNQ NTRRLSKFPYEVRQEIAPHFVDSAPVIEDDRKLYKLNVKTGDSIRDGLTPRWNDLDVNQHVNN VKFIGWILKSVPTKVFETQELCGVTLEYRRECGKDSVLESVTAMDPAKEGDRSVYQHLLRLED 15 GADITIGRTEWRPKNAGANEAISSGKTSNGNSAS

SEO ID NO: 127

CwFATB5.1a amino acid sequence

MVAAAASSAFFSVPTPGTSPKPGKFRNWPLSLSVPFKPETNHNGGFHIKANASAHPKA

NGSALNLKSGSLETQEDTSLSSPPRTFIKQLPDWSMLLSKITTVFGAAEKQLKRPGMLVEPFGV 20 DRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLI

WWTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNQ NTRRLSKFPYEVRQEIAPHFVDSAPVIEDDRKLYKLNVKTGDSIRDGLTPRWNDLDVNQHVNN VKFIGWILKSVPTKVFETQELCGVTLEYRRECGKDSVLESVTAMDPAKEGDRSVYQHLLRLED GADITIGRTEWRPKNAGANEAISSGKTSNGNSAS

SEO ID NO: 128

CcFATB2b amino acid sequence

MVTTSLASAYFSMKAVMLAPDGRGIKPRSSGLQVRAGNERNSCKVINGTKVKDTEG

LKGCSTLQGQSMLDDHFGLHGLVFRRTFAIRCYEVGPDRSTSIMAVMNHLQEAARNHAESLG LLGDGFGETLEMSKRDLIWWRRTHVAVERYPAWGDTVEVEAWVGASGNTGMRRDFLVRD 30 CKTGHILTRCTSVSVMMNMRTRRLSKIPQEVRAEIDPLFIEKVAVKEGEIKKLQKLNDSTADYI

QGGWTPRWNDLDVNQHVNNIIYVGWIFKSVPDSISENHHLSSITLEYRRECIRGNKLQSLTTVC GGSSEAGIICEHLLQLEDGSEVLRARTEWRPKHTDSFQGISERFPQQEPHK

SEO ID NO: 129

CcFATB3 amino acid sequence

MVATAAASAFFPVGAPATSSATSAKASMMPDNLDARGIKPKPASSSGLQVKANAHA

SPKINGSKVSTDTLKGEDTLTSSPAPRTFINQLPDWSMFLAAITTIFLAAEKQWTNLDWKPRRP DMLADPFGIGRFMQDGLIFRQHFAIRSYEIGADRTASIETLMNHLQETALNHVRSAGLLGDGF GATPEMSRRDLIWWTRMQVLVDRYPAWGDIVEVETWVGASGKNGMRRDWLVRDSQTGEI LTRATSVWVMMNKRTRRLSKLPEEVRGEIGPYFIEDVAIIEEDNRKLQKLNENTADNVRRGLT

PRWSDLDVNQHVNNVKYIGWILESAPGSILESHELSCMTLEYRRECGKDSVLQSMTAVSGGG

SAAGGSPESSVECDHLLQLESGPEWRGRTEWRPKSANNSRSILEMPAESL

SEO ID NO: 130

CcFATB3b amino acid sequence

MVATAAASAFFPVGAPATSSATSAKASMMPDNLDARGIKPKLASSSGLQVKANAHA

SPKINGSKVSTDTLKGEDTLTSSPAPRTFINQLPDWSMFLAAITTIFLAAEKQWTNLDWKPRRP DMLADPFGIGRFMQDGLIFRQHFAIRSYEIGADRTASIETLMNHLQETALNHVRSAGLLGDGF GATPEMSRRDLIWWTRMQVLVDRYPAWGDIVEVETWVGASGKNGMRRDWLVRDSQTGEI LTRATSVWVMMNKRTRRLSKLPEEVRGEIGPYFIEDVAIIEEDNRKLQKLNENTADNVRRGLT PRWSDLDVNQHVNNVKYIGWILESAPGSILESHELSCMTLEYRRECGKDSVLQSMTAVSGGG

SAAGGSPESSVECDHLLQLESGPEWRGRTEWRPKSANNSRSILEMPAESL

2018267601 21 Nov 2018

SEP ID NO: 131

CcFATB3c amino acid sequence

MVATAAASAFFPVGAPATSSATSAKASMMPDNLDARGIKPKPASSSGLQVKANAHA

SPKINGSKVSTDTLKGEDTLTSSPAPRTFINQLPDWSMFLAAITTIFLAAEKQWTNLDWKPRRP

DMLADPFGIGRFMQDGLIFRQHFAIRSYEIGADRTASIETLMNHLQETALNHVRSAGLLGDGF

GATPEMSRRDLIWWTRMQVLVDRYPAWGDIVEVETWVGASGKNGMRRDWLVRDSQTGEI

LTRATSVWVMMNKRTRRLSKLPEEVRGEIGPYFIEDVAIIEEDNRKLQKLNENTADNVRRGLT PRWSDLDVNQHVNNAKYIGWILESAPGSILESHELSCMTLEYRRECGKDSVLQSMTAVSGGG SAAGGSPESSVECDHLLQLESGPEWRGRTEWRPKSANNSRSILEMPAESL

SEO ID NO: 132

ChtFATBla amino acid sequence

MVAAAASSAFFSVPTPGTSTKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASAHPK

ANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRPGMLVEP FGVDRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCK 15 RDLIWWTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAM

MNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGDSIRKGLTPRWNDLDVNQ HVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVLESVTAMDTAKEGDRSLYQHL LRLEDGADITIGRTEWRPKNAGANGAISTGKTSNENSVS

SEO ID NO: 133

ChtFATBla.l amino acid sequence

MVAAAASSAFFSVPTPGTSPKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASAHPK

ANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRPGMLVEP

FGVDRIFQDGVFFRHSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCK

RDLIWWTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLIGDCRTGEILIRATSVWAM

MNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGDSIRKGLTPRWNDLDVNQ

HVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVLESVTAMDTAKEGDRSLYQHL LRLEDGADITIGRTEWRPKNAGANGALSTGKTSNGNSVS

SEO ID NO: 134

ChtFATBla.2 amino acid sequence

MVAAAASSAFFSVPTPGTSPKPGNFGNWPSNLSVPFKPESNHNGGFRVKANASAHPK

ANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRPGMLVEP FGVDRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCK RDLIWWTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAM MNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGDSIRKGLTPRWNDFDVNQ

HVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVLESVTAMDTAKEGDRSLYQHL

LRLEDGADITIGRTEWRPKNAGANGAISTGKTSNENSVS

SEO ID NO: 135

ChtFATBla.3 amino acid sequence

MVAAAASSAFFSVPTPGTSPKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASAHPK

ANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRPGMLVEP

FGVDRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCK

RDLIWWTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAM

MNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGDSIRKGLTPRWNDFDVNQ

HVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVLESVTAMDTAKEGDRSLYQHL

LRLEDGADITIGRTEWRPKNAGVNGAISTGKTSNENSVS

SEO ID NO: 136

ChtFATBla.4 amino acid sequence

MVAAAASSAFFSVPTPGTSPKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASAHPK

ANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWSMLLSKITTVFGAAERQWKRPGMLVEPF

GVDRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKR

DLIWWTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMM

2018267601 21 Nov 2018

NRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGDSIRKGLTPRWNDFDVNQHV

NNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVLESVTAMDTAKEGDRSLYQHLLR LEDGADITIGRTEWRPKNAGANGAISTGKTSNENSVS

SEO ID NO: 137

ChtFATBlb amino acid sequence

MVAAAASSAFFSVPTSGTSPKPGNFGNWPSSLSVPFKPESSHNGGFQVKANASAHPK

ANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWSMLLSKITTVFWAAERQWKRPGMLVEP FGVDRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCK RDLIWWTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAM 10 MNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGDFIRKGLTPRWNDFDVNQ

HVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVLESVTAMDTAKEGDRSLYQHL LRLEDGADITIGRTEWRPKNAGANGAISTGKTSNENSVS

SEO ID NO: 138

ChtFATB2b amino acid sequence

MWAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHP

KANGSAVSLKSGSLNTQEGTSSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQLTMLDRKSKK PDMHVDWFGLEIIVQDGLVFRESFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGF GRTPEMCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIR ATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPEW 20 NDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGD

RSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS

SEO ID NO: 139

ChtFATB2a amino acid sequence

MWAAAASSAFFPVPAPGTTSKPGKFGNWPSSLSPSFKPKSNPNGGFQVKANASAHP

KANGSAVSLKSGSLNTKEDTPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQLTMLDRKSKK

PDMHVDWFGLEIIVQDWLVFRESFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGF

GRTPEMCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIR

ATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDAPPLIEDNDRKLHKFDVKTGDSICKGLTPEW NDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGD 3 0 RSQYQHLLRLEDGTDIMKGRTE WRPKNAGTNGAISTGKTSNGNSVS

SEO ID NO: 140

ChtFATB2c amino acid sequence

MWAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHP

KANGSAVSLKSGSLNTKEDTPSSPPPRTFLNQLPDWNRLRTAITTVFVAAEKQLTMLDRKSKK

PDMHVDWFGLEIIVQDGLVFRESFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGF

GRTPEMCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIR

ATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPEW NDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGD RSQYQHLLRLEDGTDIMKGRTE WRPKNAGTNGAISTGKTSNGNSVS

SEO ID NO: 141

ChtFATB2d amino acid sequence

MWAAAASSAFFPVPAPGTTSKPGKFGNWPSSLSPSFKPKSNPNGGFQVKANASAHP

KANGSAVSLKSGSLNTQEDTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRP

DMLVDLFGLESIVQDGLVFRESYSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFG

RTPEMCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRA

TSIWAMMNQNTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSIRKGLTPGWN DLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRRECGRESVLESVTAMNPSKVGDRS QYQHLLRLEDGADIMKGRTE WRPKNAGTNGAISTGKTSNGNSVS

2018267601 21 Nov 2018

SEP ID NO: 142

ChtFATB2e amino acid sequence

MWAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHP

KANGSAVSLKSGSLNTQEDTSSSPPPQTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRP

DMLVDWFGLESIVQDGLVFRESYSIRSYEISADRTASIETVMNLLQETSLNHCKSMGILNDGFG

RTPEMCKRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRA TSIWAMMNQNTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSIRKGLTPGWN DLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRRECGRDSVLESVTAMNPSKVGDRS QYQHLLRLEDGADIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS

SEO ID NO: 143

ChtFATB2f amino acid sequence

MWAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHP

KANGSAVSLKSGSLNTQEGTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRP DMLVDWFGLESIVQDGLVFRESYSIRSYEISADRTASIETVMNLLQETSLNHCKSMGILNDGFG 15 RTPEMCKRDLIWVLTKMQILVNRYPNWGDTVEINS WF SQSGKIGMGRNWLISDCNTGEILIRA

TSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPEWN DLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGDR SQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS

SEO ID NO: 144

ChtFATB2g amino acid sequence

MWAATASSAFFPVPVPGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPK

ANGSAVSLKSGSLNTQEDTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRPD

MLVDWFGLESIVQDGLVFREIYSIRSYEISADRTTSIETVMNLLQETSLNHCKSMGILNDGFGRT

PEMCKRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSI

WAMMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSICKGLTPEWNDL DVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRECGRDSVLESVTAMDPSKVGDRSQ YQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNANSVS

SEO ID NO: 145

ChtFATB2h amino acid sequence

MWAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHP

KANGSAVSLKSGSLNTQEGTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRP

DMLVDWFGLESIVQDGLVFRESYSIRSYEISADRTASIETVMNLLQETSLNHCKSMGILNDGFG

RTPEMCKRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRA

TSIWAMMNQNTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHKFDVKTGDSIRKGLTPGWN

DLDVNQHVSNVKYIGWILESIPTEVLETQELCSLTLEYRRECGRESVLESVTAMNPSKVGDRSQ YQHLLRLEDGADIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS

SEO ID NO: 146

ChtFATB3a amino acid sequence

MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKIN

GSSVSLKSCSLKTHEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD

MLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNEGFG RTPEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEIL TRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKLPKLDEKTADSIRKGLTP KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKG 45 FGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIASGETSPGNS

SEO ID NO: 147

ChtFATB3b amino acid sequence

MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKIN

GSSVSLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD

MLVDPFGFGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLIEGFGR

TPEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILT

2018267601 21 Nov 2018

RASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKLPKLDEKTADSIRKGLTPK

WNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKGF GPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIASGETSPGNS

SEO ID NO: 148

ChtFATB3c amino acid sequence

MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKIN GSSVSLKSCSLKTHEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNEGFG RTPEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEIL 10 TRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTP KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSEKG FGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGAIAFGETSPGDS

SEO ID NO: 149

ChtFATB3d amino acid sequence

MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKIN

GSSVSLKSCSLKTHEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIKTVMNHLQETALNHVKSAGLLNEGFG RTPEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEIL TRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKLPKLDEKTADSIRKGLTP

KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKG

FGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIASGETSPGNS

SEO ID NO: 150

ChtFATB3e amino acid sequence

MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKIN

GSSVSLKSGSLKTHEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD

MLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNEGFG RTPEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEIL TRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKLPKLDEKTADSIRKGLTP KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKG 3 0 FGPQFQHLLRLEDGGEIVKGRTE WRPKTAGINGTIASGETSPGNS

SEO ID NO: 151

ChtFATB3f amino acid sequence

MVATAASSAFFPVPSPDTSSRLGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKIN

GSSVSLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD

MPVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNEGFG

RTPEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEIL TRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKLPKLDEKTADSIRKGLTP KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSEKG FGPQFQHLLRLEDGGEIVKGRTE WRPKTAGINGTIASGETSPGNS

SEO ID NO: 152

ChtFATB3g amino acid sequence

MVATAASSAFFPVPSPDTSSRAGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKIN GSSVSLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNEGFG 45 RTPEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEIL TRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKLPKLDEKTADSIRKGLTP KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKG FGPQFQHLLRLEDGGEIVKGRTE WRPKTAGINGTIASGETSPGNS

2018267601 21 Nov 2018

SEP ID NO: 153

ChsFATBl amino acid sequence

MVATNAAAFSAYTFFLTSPTHGYSSKRLADTQNGYPGTSLKSKSTPPPAAAAARNGA

LPLLASICKCPKKADGSMQLDSSLVFGFQFYIRSYEVGADQTVSIQTVLNYLQEAAINHVQSAG

YFGDSFGATPEMTKRNLIWVITKMQVLVDRYPAWGDWQVDTWTCSSGKNSMQRDWFVRD

LKTGDIITRASSVWVLMNRLTRKLSKIPEAVLEEAKLFVMNTAPTVDDNRKLPKLDGSSADYV LSGLTPRWSDLDMNQHVNNVKYIAWILESVPQSIPETHKLSAITVEYRRECGKNSVLQSLTNV SGDGITCGNSIIECHHLLQLETGPEILLARTEWISKEPGFRGAPIQAEKVYNNK

SEQ ID NO: 154

ChsFATB2 amino acid sequence

MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAPPKIN GSSVGLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFG RTLEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEI 15 LTRASSVWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTP KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKG SGSQFQHLLRLEDGGEIVKGRTEWRPKTAGINGPIASGETSPGDSS

SEO ID NO: 155

ChsFalB2b amino acid sequence

MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAPPKIN

GSSVGLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD MLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFG RTLEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEI LTRASSKSQIMLPLHYCSVWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLD 25 EKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESV

LESLTAVDPSGKGSGSQFQHLLRLEDGGEIVKGRTEWRPKTAGINGPIASGETSPGDSS

SEO ID NO: 156

ChsFalB2c amino acid sequence

MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAPPKIN

GSSVGLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD

MLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFG RTLEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEI LTRASSVWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTP KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKG 35 SGSQFQHLMRLEDGGEIVKGRTEWRPKTAGINGPIASGETSPGDSS

SEO ID NO: 157

ChsFalB2d amino acid sequence

MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAPPKIN

GSSVGLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPD

MLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFG

RTPEMYKRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEIL TRASSVWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTP KWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGKG SGSQFQHLLRLEDGGEIVKGRTEWRPKTAGINGPIASGETSPGDSS

SEO ID NO: 158

Chs FATB3 amino acid sequence

MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASARPK

ANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRP

DMLMDPFGVDRWQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFG

RTPEMCKRDLIWWTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDCHTGEILIR

ATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRW

2018267601 21 Nov 2018

NDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRRECGRDSVLESVTAMDPSKEGD

RSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS

SEO ID NO: 159

ChsFatb3b amino acid sequence

MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASARPK

ANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRP

DMLMDPFGVDRWQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFG RTPEMCKRDLIWWTKMHIEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDFHTGDILIR ATSVCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRW 10 NDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRRECGRDSVLESVTAMDPSKEGD RSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS

SEO ID NO: 160

ChsFatB3c amino acid sequence

MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASARPK

ANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRP

DMLMDPFGVDRWQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFG RTPEMCKRDLIWWTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDCHTGEILIR ATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRW NDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRQECGRDSVLESVTAMDPSKEGD 20 RSLYQHLLRLEDGTDIAKGRTKWRPKNAGKTSNGNSIS

SEO ID NO: 161

ChsFATB3d amino acid sequence

MVAAEASSALFSVRTPGTSPKPGKFGNWPSSLSVPFKSKSNHNGGFQVKANASARPK

ANGSAVSLKSGSLDTQEDASSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKR

SDMLMDPFGVDRWQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGF

GRTPEMCKRDLIWWTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDCHTGEILI

RATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPR WNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRRECGRDSVLESVTAMDPSKEG DRSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS

SEO ID NO: 162

ChsFATB3e amino acid sequence

MVAAEASSALFSVRTPGTSPKPGKFGNWPSSLSVPFKSKSNHNGGFQVKANASARPK

ANGSAVSLKSGSLDTQEDASSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKR SDMLMDPFGVDRWQDGWFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGF 35 GRTPEMCKRDLIWWTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDCHTGEILI

RATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPR WNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRRECGRDSVLESVTAMDPSKEG DRSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS

SEO ID NO: 163

ChsFATB3f amino acid sequence

MVAAEASSALFSVRTPGTSPKPGKFGNWPSSLSVPFKSKSNHNGGFQVKANASARPK

ANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRP

DMLMDPFGVDRWQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFG RTPEMCKRDLIWWTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDCHTGEILIR 45 ATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRW

NDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRRECGRDSVLESVTAMDPSKEGD RSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS

SEO ID NO: 164

ChsFATB3g amino acid sequence

MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASARPK

2018267601 21 Nov 2018

ANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRP

DMLMDPFGVDRWQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFG RTPEMCKRDLIWWTKMHIEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDFHTGDILIR

ATSVCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRW

NDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRQECGRDSVLESVTAMDPSKEGD RSLYQHLLRLEDGTDIAKGRTKWRPKNAGKTSNGNSIS

SEO ID NO: 165

ChsFATB3h amino acid sequence

MVAAEASSALFSVRTPGTSPKPGKFGNWPSSLSVPFKSKSNHNGGFQVKANASARPK

ANGSAVSLKSGSLDTQEDASSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKR

SDMLMDPFGVDRWQDGWFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGF GRTPEMCKRDLIWWTKMHIEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDFHTGDILI RATSVCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPR WNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRQECGRDSVLESVTAMDPSKEG 15 DRSLYQHLLRLEDGTDIAKGRTKWRPKNAGKTSNGNSIS

SEO ID NO: 166

ChsFATB3i amino acid sequence

MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASARPK

ANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRP

DMLMDPFGVDRWQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFG

RTPEMCKRDLIWWTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDCHTGEILIR

ATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRW NDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRRECGGDSVLESVTAMDPSKEGD RSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS

SEO ID NO: 167

ChsFATB3i amino acid sequence

MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASARPK

ANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRP DMLMDPFGVDRWQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFG 30 RTPEMCKRDLIWWTKMHIEVNRYPTWGDTIEVNTWVSESGKTGMGRDWLISDFHTGDILIR

ATSVCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLHKLDVKTGDSICNGLTPRW NDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRQECGRDSVLESVTAMDPSKEGD RSLYQHLLRLEDGTDIAKGRTKWRPKNAGKTSNGNSIS

SEO ID NO: 168

CcalcFATBl (Cuphea calcarata FATB 1)

MVAAAATSAFFPVPAPGTSPNPRKFGSWPSSLSPSLPKSIPNGGFQVKANASAHPKANGSAVSL

KSGSLNTQENTSSSPPPRTFLHQLPDWSRLLTAITTVFVKSKRPDMHDRKSKRPDMLVDLFGLE SSVQDALVFRQSFSIRSYEIGTDRTASIETLMNHLQETSLNHCKSTGILLDGFGRTLEMCKRELI WWIKMQIQVNRYPAWGDTVEINTRFSRLGKIGMGRDWLISDCNTGEILIRATSEYAMMNQK 40 TRRLSKLPYEVHQEIAPLFVDSPPVIEDNDLKVHKFEVKTGDSIQKGLSPGWNDLDVNQHVSN

VKYIGWILESMPTEVLETQELCSLALEYRRECGRDSVLESVTAMDPSKVGGRSQYQHLLRLED GTAIVNGITEWRPKNAGANGAISTGKTSNGNSVS

SEO ID NO: 169

ChookFATB4 (Cuphea hookeriana FATB4)

MVAAAATSAFFPVPAPGTSPNPRKFGSWPSSLSPSLPNSIPNGGFQVKANASAHPKANGSAVSL

KSGSLNTQENTSSSPPPRTFLHQLPDWSRLLTAITTVFVKSKRPDMHDRKSKRPDMLVDLFGLE SSVQDALVFRQRFSIRSYEIGTDRTASMETLMNHLQETSLNHCKSTGILLDGFGRTLEMCKREL IWWIKMQIQVNRYPAWGDTVEINTRFSRLGKIGMGRDWLISDCNTGEILIRATSEYAMMNQK TRRLSKLPYEVRQEIAPLFVDSPPVIEDNDLKVHKFEVKTGDSIHKGLTPGWNDLDVNQHVNN

2018267601 21 Nov 2018

VKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAMDPSGGGYGSQFQHLLRLEDG GEIVKGRTEWRPKNGVINGWPTGESSPGDYS

SEO ID NO: 170

CaFATBl (Cuphea avigera var. pulcherrima FATB1)

MVAAAASSAFFSVPVPGTSPKPGKFRIWPSSLSPSFKPKPIPNGGLQVKANSRAHPKANGSAVS LKSGSLNTQEDTSSSPPPRTFLHQLPDWSRLLTAITTVFVKSKRPDMHDRKSKRPDMLMDSFG LESIVQEGLEFRQSFSIRSYEIGTDRTASIETLMNYLQETSLNHCKSTGILLDGFGRTPEMCKRDL IWWTKMKIKVNRYPAWGDTVEINTWFSRLGKIGKGRDWLISDCNTGEILIRATSAYATMNQ KTRRLSKLPYEVHQEIAPLFVDSPPVIEDNDLKLHKFEVKTGDSIHKGLTPGWNDLDVNQHVS

NVKYIGWILESMPTEVLETQELCSLALEYRRECGRDSVLESVTAMDPTKVGGRSQYQHLLRLE DGTDIVKCRTEWRPKNPGANGAISTGKTSNGNSVS

SEO ID NO: 171

CpauFATBl (Cupheapaucipetala FATB1)

MVAAAASSAFFPVPAPGTSPKPGKSGNWPSSLSPSIKPMSIPNGGFQVKANASAHPKANGSAV

NLKSGSLNTQEDTS SSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMRDRKSKRPDMLVD

SVGLKSWLDGLVSRQIFSIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMC KNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSHSGKIGMASDWLITDCNTGEILIRATSVWA MMNQKTRRFSRLPYEVRQELTPHYVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLDV NQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAMDPSEDEGRSQYKH

LLRLEDGTDIVKGRTEWRPKNAGTNGAISTAKPSNGNSVS

SEO ID NO: 172

CprocFATBl (Cuphea procumbens FATB1)

MVAAAASSAFFPAPAPGSSPKPGKSGNWPSSLSPSFKSKSIPYGRFQVKANASAHPKANGSAV

NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVD

SVGLKNIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGM CKNDLIWVLTKMQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVW AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLD VNQHVSNVKYIGWILESMPIEVLEAQELCSLTVEYRRECGMDSVLESVTAVDPSEDGGRSQYN HLLRLEDGTDWKGRTEWRPKNAETNGAISPGNTSNGNSIS

SEO ID NO: 173

CprocFATB2 (Cuphea procumbens FATB2)

MVAAAASSAFFPAPAPGSSPKPGKSGNWPSSLSPSFKSKSIPYGRFQVKANASAHPKANGSAV NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVD SVGLKNIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGM

CKNDLIWVLTKMQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVW AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLD VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRQECGRESVLESLTAVDPSGKGFGSQFQH LLRLEDGGEIVKGRTEWRPKTAGINGAIASGETSPGDF

SEO ID NO: 174

CprocFATB3 (Cuphea procumbens FATB3)

MVAAAASSAFFPAPAPGSSPKPGKSGNWPSSLSPSFKSKSIPYGRFQVKANASAHPKANGSAV NLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVD SVGLKNIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGM

CKNDLIWVLTKMQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVW

AMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLD

VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAVDPSGEGGYGSQFQ HLLRLEDGGEIVKGRTEWRPKNAGINGVLPTGE

2018267601 21 Nov 2018

SEP ID NO: 175

CigneaFATBl (Cuphea ignea FATB1)

PGTSRKTGKFGNWPSSLSPSFKPKSIPNGGFOVKANARAHPKANGSAVSLKSVSLNTQEDTSLS

PPPRAFLNQLPDWRMLRTALTTVFVAAEKQWTMLDRKSKRPDMLVDSFGLESIVQEGLVFRQ

SFSIRSYEIGIDRTASIETLMNHLQETSLNQCKSAGILHDGFGRTLEMCKRDLIWWTKMQIKV

NRYPAWGDTVEISTRFSRLGKIGMGRDWLICDCNTGEILIRATSAYAMMNQKTRRLSKLPNEV RQEIAPLFVDSDPVIEENDMKLHKFEVKTGDSICKGLTPRWSDLDVNQHVSNVKYIGWILESM PTEVLETQELCSLALEYRRECGRDSVLESVTSMDPSKVGGWSQYQHLLRLEDGADIVKGRTE WRPKNAGANGAISTGKT

SEO ID NO: 176

CcalcFATBl (Cuphea calcarata FATB1)

Imatastfsafnarcgdlrrsagsgprrparplpvraa^nasahpkangsavslksgsletqed

NSSSSRPPRTFIKQLPDWSMLLSAITTVFVAAEKQWTMFDRKSKRSDMLVDPFWDRIVQDGV LFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSMGLLYEGFGRTPEMCKRDLIWWTKIH 15 IKVNRYPTWGDTIEVTTWVSESGKNGMGRDWLISDCHTGEILIRATSVWAMMNQTTRRLSKF

PYELRQEIAPHFVDSDPVIEDNRRLLNFDVKTGDSIRKGLTPRWNDLDVNQHVNNVKYIGWIL ESVPTEVFDTRELCGLTLEYRQECGRGSVLESVTAMDPSKEGDRSLYQHLLRLEDGTDIVKGR TEWRPKNAGTNGPVSTRKTTNGSSVS

SEO ID NO: 177

ChookFATB4 (Cuphea hookeriana FATB4) |matastfsafnarcgdlrrsagsgprrparplpvraai|nasahpkangsavslksgslntqen

TSSSPPPRTFLHQLPDWSRLLTAITTVFVKSKRPDMHDRKSKRPDMLVDLFGLESSVQDALVFR QRFSIRSYEIGTDRTASMETLMNHLQETSLNHCKSTGILLDGFGRTLEMCKRELIWWIKMQIQ VNRYPAWGDTVEINTRFSRLGKIGMGRDWLISDCNTGEILIRATSEYAMMNQKTRRLSKLPYE 25 VRQEIAPLFVDSPPVIEDNDLKVHKFEVKTGDSIHKGLTPGWNDLDVNQHVNNVKYIGWILES

TPPEVLETQELCSLTLEYRRECGRESVLESLTAMDPSGGGYGSQFQHLLRLEDGGEIVKGRTE WRPKNGVINGWPTGESSPGDYS

SEO ID NO: 178

CaFATBl (Cuphea avigera var. pulcherrima FATB1)

0 |MATASTFSAFNARCGDLRRSAGSGPRRPARPLPVRAAI^SRAHPKANGSAVSLKSGSLNTQED TSSSPPPRTFLHQLPDWSRLLTAITTVFVKSKRPDMHDRKSKRPDMLMDSFGLESIVQEGLEFR QSFSIRSYEIGTDRTASIETLMNYLQETSLNHCKSTGILLDGFGRTPEMCKRDLIWWTKMKIK VNRYPAWGDTVEINTWFSRLGKIGKGRDWLISDCNTGEILIRATSAYATMNQKTRRLSKLPYE VHQEIAPLFVDSPPVIEDNDLKLHKFEVKTGDSIHKGLTPGWNDLDVNQHVSNVKYIGWILES

MPTEVLETQELCSLALEYRRECGRDSVLESVTAMDPTKVGGRSQYQHLLRLEDGTDIVKCRTE WRPKNPGANGAISTGKTSNGNSVS

SEO ID NO: 179

CpauFATBl (Cupheapaucipetala FATB1) |MATASTFSAFNARCGDLRRSAGSGPRRPARPLPVRAA|4ASAHPKANGSAVNLKSGSLNTQE

DTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMRDRKSKRPDMLVDSVGLKSWLDG

LVSRQIFSIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIWVLTK MQIMVNRYPTWGDTVEINTWFSHSGKIGMASDWLITDCNTGEILIRATSVWAMMNQKTRRFS RLPYEVRQELTPHYVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLDVNQHVSNVKYI GWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAMDPSEDEGRSQYKHLLRLEDGTDI 45 VKGRTEWRPKNAGTNGAISTAKPSNGNSVS

2018267601 21 Nov 2018

SEP ID NO: 180

CprocFATBl (Cuphea procumbens FATB1)

Imatastfsafnarcgdlrrsagsgprrparplpvraa^nasahpkangsavnlksgslntqe

DTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVDSVGLKNIVRDG

LVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIWVLTK

MQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVWAMMNQKTRRFS RLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLDVNQHVSNVKYI

GWILESMPIEVLEAQELCSLTVEYRRECGMDSVLESVTAVDPSEDGGRSQYNHLLRLEDGTDV VKGRTEWRPKNAETNGAISPGNTSNGNSIS

SEO ID NO: 181

CprocFATB2 (Cuphea procumbens FATB2)

Imatastfsafnarcgdlrrsagsgprrparplpvraa^nasahpkangsavnlksgslntqe

DTSSSPPPRAFLNQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLVDSVGLKNIVRDG lvsrqsflirsyeigadrtasietlmnhlqetsinhckslgllndgfgrtpgmckndliwvltk 15 mqimvnrypawgdtveintwfsqsgkigmgsdwlisdcntgeiliratsvwammnqktrrfs rlpyevrqeltphfvdsphviedndrklhkfdvktgdsirkgltprwndldvnqhvnnvkyi gwilestppevletqelcsltleyrqecgresvlesltavdpsgkgfgsqfqhllrledggeiv kgrtewrpktagingaiasgetspgdf

SEO ID NO: 182

CprocFATB3 (Cuphea procumbens FATB3)

Imatastfsafnarcgdlrrsagsgprrparplpvraa^nasahpkangsavnlksgslntqe dtssspppraflnqlpdwsmllsaittvfvaaekqwtmldrkskrpdmlvdsvglknivrdg lvsrqsflirsyeigadrtasietlmnhlqetsinhckslgllndgfgrtpgmckndliwvltk mqimvnrypawgdtveintwfsqsgkigmgsdwlisdcntgeiliratsvwammnqktrrfs 25 rlpyevrqeltphfvdsphviedndrklhkfdvktgdsirkgltprwndldvnqhvnnvkyi gwilestppevletqelcsltleyrrecgresvlesltavdpsgeggygsqfqhllrledggei vkgrtewrpknagingvlptge

SEO ID NO: 183

CigneaFATBl (Cuphea ignea FATB1)__________________

0 |matastfsafnarcgdlrrsagsgprrparplpvraai^arahpkangsavslksvslntqed tslspppraflnqlpdwrmlrtalttvfvaaekqwtmldrkskrpdmlvdsfglesivqegl vfrqsfsirsyeigidrtasietlmnhlqetslnqcksagilhdgfgrtlemckrdliwwtkm qikvnrypawgdtveistrfsrlgkigmgrdwlicdcntgeiliratsayammnqktrrlskl pnevrqeiaplfvdsdpvieendmklhkfevktgdsickgltprwsdldvnqhvsnvkyigwi lesmptevletqelcslaleyrrecgrdsvlesvtsmdpskvggwsqyqhllrledgadivk grtewrpknagangaistgkt

2018267601 21 Nov 2018

SEP ID NO: 185

CgFATBlb (Cuphea glossostoma FATB1 C170F,M198T,T374S variant)

MVAAAASSAFFPSPAPGSSPKPGNRPSSLSPSFKPKSIPNGAFQVKANASAHPKANGSAVNLKS GSLNTQEDSSSSPSPRAFLNQLPDWSVLLTAITTVFVAAEKQWTMLDRKSKRPDVLVDSVGLK

SIVQDGLVSRQSFSIRSYEIGADRTASIETLMNHLQETSINHFKSLGLLNDGFGRTPGMCKNDLI WVLTKTQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILIRATSVWAMMNQ KTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLDVNQHVS NVKYIGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVSAMDPSEDGGRSQYNHLLRLE DGTDWKGRTEWRPKNAGTNGAISTTKTSNGNSVS

SEO ID NO: 186

Umbellularia califomica UcFATB3 amino acid sequence

MVATAAASAFFPVGSPATSSATSAKASMMPDNLDARGIKPKPASSSGLQVKANAHASPKINGS KVSTDTLKGEDTLTSSPAPRTFINQLPDWSMFLAAITTIFLAAEKQWTNLDWKPRRPDMLADP

FGIGRFMQDGLIFRQHFAIRSYEIGADRTASIETLMNHLQETALNHVRSAGLLGDGFGATPEMS RRDLIWWTRMQVLVDRYPAWGDIVEVETWVGASGKNGMRRDWLVRDSQTGEILTRATSV WVMMNKRTRRLSKIPEEVRGEIGPYFMENVAIIEEDSRKLQKLNENIIEEDSRKLQKLNENTAD NVRRGLTPRWSDLDVNQHVNNVKYIGWILESAPGSILESHELSCMTLEYRRECGKDSVLQSM TVVSGGGSAAGGSPESSVECDHLLQLESGPEWKARTEWRPKSANNPRSILEMPAESS*

SEO ID NO: 187

Cuphea carthagenensis CCrFATB2c (V138L variant of FATB2)

MVAAAASSAFFPVTTPGTSRKPGKFGNWLSSLSPPFRPKSIPSGGFQVKANASAHPKANGSAV SLKSGSLNTQEDTSSSPPPRAFINQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRSDMLVDS

FGMERIVQDGLLFRQSFSIRSYEIGADRRASIETLMNHLQETSLNHCKSIRLLNEGFGRTPEMCK RDLIWWTRMHIMVNRYPTWGDTVEINTWVSQSGKNGMGRDWLISDCNTGEILIRATSAWA MMNQKTRRLSKLPYEVSQEIAPHFVDSPPVIEDGDRKLHKFDVKTGDSIRKGLTPRWNDLDV NQHVNNVKYIGWILESMPTEVLETHELCFLTLEYRRECGRDSVLESVTAMDPSNEGGRSHYQ HLLRLEDGTDIVKGRTEWRPKNARNIGAISTGKTSNGNPAS*

SEO ID NO: 188

Cuphea carthagenensis CCrFATB2

MVAAAASSAFFPVTTPGTSRKPGKFGNWLSSLSPPFRPKSIPSGGFQVKANASAHPKANGSAV SLKSGSLNTQEDTSSSPPPRAFINQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRSDMLVDS

FGMERIVQDGLVFRQSFSIRSYEIGADRRASIETLMNHLQETSLNHCKSIRLLNEGFGRTPEMCK RDLIWWTRMHIMVNRYPTWGDTVEINTWVSQSGKNGMGRDWLISDCNTGEILIRATSAWA MMNQKTRRLSKLPYEVSQEIAPHFVDSPPVIEDGDRKLHKFDVKTGDSIRKGLTPRWNDLDV NQHVNNVKYIGWILESMPTEVLETHELCFLTLEYRRECGRDSVLESVTAMDPSNEGGRSHYQ HLLRLEDGTDIVKGRTEWRPKNARNIGAISTGKTSNGNPAS*

SEO ID NO: 189

CcrFATB2b

MVAAAASSAFFPVTTPGTSRKPGKFGNWLSSLSPPFRPKSIPSGGFQVKANASAHPKANGSAV

SLKSGSLNTQEDTSSSPPPRAFINQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRSDMLVDS FGMERIVQDGLVFRQSFSIRSYEIGADRRASIETLMNHLQETSLNHCKSIRLLNEGFGRTPEMCK RDLIWVFTRMHIMVNRYPTWGDTVEINTWVSQSGKNGMGRDWLISDCNTGEILIRATSAWA MMNQKTRRLSKLPYEVSQEIAPHFVDSPPVIEDGDRKLHKFDVKTGDSIRKGLTPRWNDLDV NQHVNNVKYIGWILESMPTEVLETHELCFLTLEYRRECGRDSVLESVTAMDPSNEGGRSHYQ

0 HLLRLEDGTDIVKGRTE WRPKNARNIGAIPTGKTSNGNPAS *

2018267601 21 Nov 2018

SEP ID NO: 190

CcrFATBl

MVATAASSAFFPVPSPDSSSRPGKLGNGPSSLSPLKPKSTPNGGLQVKANASAPPKINGSSVGL

KSSSLKTQDDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLTDPF GLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFGRTPEMY KRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSV WVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTPKWNDLD VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGKESVLESLTAVDPSGKGWGSHFQ

HLLRLEDGGEIVKGRTEWRPKNAGINGAVAFEETSPGDS*

SEO ID NO: 191

CcrFATBlb

MVATAASSAFFPVPSPDSSSRPGKLGNGPSSLSPLKPKSTPNGGLQVKANASAPPKINGSSVGL KSSSLKTQDDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLTDPF GLGRIAQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFGRTPEMY KRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSV WVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTPKWNDLD

VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGKESVLESLTAVDPSGKGWGSHFQ HLLRLEDGGEIVKGRTEWRPKNAGINGAVAFEETSPGDS*

SEO ID NO: 192

CCrFATBlc

MVATAASSAFFPVPSPDSSSRPGKLGNGPSSLSPLKPKSTPNGGLQVKANASAPPKINGSSVGL KSSSLKTQDDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLTDPF GLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFGRTPEMY KRDLIWWAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSV WVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTPKWNDLD

VNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGKESVLESLTAVDPSGKGWGSHFQ HLLRLEDGGEIVKGRTEWRPKNA*

Claims (21)

1. What is claimed is:

   1. A nucleic acid construct comprising a regulatory element and a FatB gene expressing an active acyl-ACP thioesterase operable to produce an altered

   5 fatty acid profile in an oil produced by a cell expressing the nucleic acid construct, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 5 of Table la, the sequence having at least 94.6% sequence identity with each of SEQ ID NOs: 88, 82, 85, and 103, and optionally wherein the fatty acid of the oil is enriched in C8 and CIO fatty

   10 acids.
2. 2. A nucleic acid construct comprising a regulatory element and a FatB gene expressing an active acyl-ACP thioesterase operable to produce an altered fatty acid profile in an oil produced by a cell expressing the nucleic acid

   15 construct, wherein the FatB gene expresses a protein having an amino acid sequence falling within one of clades 1-12 of Table la.
3. 3. The nucleic acid construct of claim 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 1 of Table la, the

   20 sequence having at least 85.9% sequence identity with each of SEQ ID NOs:

   19, 161, 22, and 160, and optionally wherein the fatty acid of the oil is enriched in C14 and C16 fatty acids.
4. 4. The nucleic acid construct of claim 2, wherein the FatB gene expresses a

   25 protein having an amino acid sequence falling within clade 2 of Table la, the sequence having at least 89.5% sequence identity with each of SEQ ID NOs: 134-136, 132, 133, 137, 124, 122, 123, 125, and optionally wherein the fatty acid of the oil is enriched in C12 and C14 fatty acids.

   30 5. The nucleic acid construct of claim 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 3 of Table la, the sequence having at least 92.5% sequence identity with each of SEQ ID NOs:

   101

   2018267601 21 Nov 2018

   126 and 127, and optionally wherein the fatty acid of the oil is enriched in C12 and C14 fatty acids.

   6. The nucleic acid construct of claim 2, wherein the FatB gene expresses a
5. 5 protein having an amino acid sequence falling within clade 4 of Table la, the sequence having at least 83.8% sequence identity with SEQ ID NO: 79, and optionally wherein the fatty acid of the oil is enriched in C12 and C14 fatty acids.

   10 7. The nucleic acid construct of claim 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 6 of Table la, the sequence having at least 99.9% sequence identity with each of SEQ ID NOs: 111 and 110, and optionally wherein the fatty acid of the oil is enriched in C10 fatty acids.
6. 8. The nucleic acid construct of claim 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 7 of Table la, the sequence having at least 89.5% sequence identity with each of SEQ ID NOs: 73, 106, 185, 172, 171, 173, 174, and optionally wherein the fatty acid of the

   20 oil is enriched in C10 and C12 fatty acids.
7. 9. The nucleic acid construct of claim 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 8 of Table la, the sequence having at least 85.9% sequence identity with each of SEQ ID NOs:

   25 112, 113, 142, 145, 143, 144, 139, 140, 138, 141, and optionally wherein the fatty acid of the oil is enriched in C12 and C14 fatty acids.
8. 10. The nucleic acid construct of claim 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 9 of Table la, the

   30 sequence having at least 83.8% sequence identity with each of SEQ ID NOs:

   187-189, and optionally wherein the fatty acid of the oil is enriched in C12 and C14 fatty acids.

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9. 11. The nucleic acid construct of claim 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 10 of Table la, the sequence having at least 95.9% sequence identity with each of SEQ ID NOs: 147, 149, 146, 150, 152, 151, 148, 154, 156, 155, 157, 108, 75, 190, 191, and

   5 192, and optionally wherein the fatty acid of the oil is enriched in C14 and

   C16 fatty acids.
10. 12. The nucleic acid construct of claim 2, wherein the FatB gene expresses a protein having an amino acid sequence falling within clade 11 of Table la, the

    10 sequence having at least 88.7% sequence identity with SEQ ID NO: 121, and optionally wherein the fatty acid of the oil is enriched in C14 and C16 fatty acids.
11. 13. The nucleic acid construct of claim 2, wherein the FatB gene expresses a
12. 15 protein having an amino acid sequence falling within clade 12 of Table la, the sequence having at least 72.8% sequence identity with each of SEQ ID NOs: 129 and 186, and optionally wherein the fatty acid of the oil is enriched in C16 fatty acids.

    20 14. An isolated nucleic acid or recombinant DNA construct comprising a nucleic acid, wherein the nucleic acid has at least 70% sequence identity to any of SEQ ID NOS: 2, 3, 5, 6, 8, 9, 11, 12, 14, 15, 17, 18, 20, 21, 23, 24, 26, 27, 29, 30, 32, 33, 35, 36, 38, 39, 41, 42, 44, 45, 47, 48, 50, 51, 53, 54, 56, 57, 59, 60, 62, 63, 65, 66, 68, 69, 71, 72, 74, 76, 78, 80, 81, 83, 84, 86, 87, 89, 90, 92, 93,

    25 95, 96, 98, 99, 101, 102, 104, 105, 107, 109 or any equivalent sequences by virtue of the degeneracy of the genetic code.

    15. An isolated nucleic acid sequence encoding a protein or a host cell expressing a protein having at least 70% sequence identity to any of SEQ ID NOS: 1, 4,

    30 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67,

    70, 73, 75, 77, 79, 82, 85, 88, 91, 94, 97, 100, 103, 106, 108, 110-192 or a fragment thereof having acyl-ACP thioesterase activity.

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13. 16. The isolated nucleic acid of claim 15, wherein, the protein has acyl-ACP thioesterase activity operable to alter the fatty acid profile of an oil produced by a recombinant cell comprising that sequence.

    5
14. 17. A method of producing a recombinant cell that produces an altered fatty acid profile, the method comprising transforming the cell with a nucleic acid according to any of claims 1-3.
15. 18. A host cell produced by the method of claim 17.
16. 19. The host cell of claim 18, wherein the host cell is selected from a plant cell, a microbial cell, and a microalgal cell.
17. 20. A method for producing an oil or oil-derived product, the method comprising

    15 cultivating a host cell of claim 5 or 6, and extracting oil produced thereby, optionally wherein the cultivation is heterotrophic growth on sugar.
18. 21. The method of claim 20, further comprising producing a fatty acid, fuel, chemical, or other oil-derived product from the oil.
19. 22. An oil produced by the method of claim 20, optionally having a fatty acid profile comprising at least 20% C8, C10, C12, C14 or C16 fatty acids.
20. 23. An oil-derived product produced by the method of claim 21.
21. 24. The oil of claim 23, wherein the oil is produced by a microalgae and optionally, lacks C24-alpha sterols.