BR112015023192A2 - tioesterases e células para a produção de óleos personalizados - Google Patents

tioesterases e células para a produção de óleos personalizados

Info

Publication number
BR112015023192A2
BR112015023192A2 BR112015023192A BR112015023192A BR112015023192A2 BR 112015023192 A2 BR112015023192 A2 BR 112015023192A2 BR 112015023192 A BR112015023192 A BR 112015023192A BR 112015023192 A BR112015023192 A BR 112015023192A BR 112015023192 A2 BR112015023192 A2 BR 112015023192A2
Authority
BR
Brazil
Prior art keywords
genes
cells
fatty acid
useful
host cells
Prior art date
Application number
BR112015023192A
Other languages
English (en)
Other versions
BR112015023192A8 (pt
Inventor
N Rudenko George
Casolari Jason
Franklin Scott
Original Assignee
Solazyme Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/837,996 external-priority patent/US9290749B2/en
Application filed by Solazyme Inc filed Critical Solazyme Inc
Publication of BR112015023192A2 publication Critical patent/BR112015023192A2/pt
Publication of BR112015023192A8 publication Critical patent/BR112015023192A8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • C12Y301/02014—Oleoyl-[acyl-carrier-protein] hydrolase (3.1.2.14), i.e. ACP-thioesterase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Fats And Perfumes (AREA)
  • Edible Oils And Fats (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

abstract the invention features plant acyl-acp thioesterase genes of the fatb class and proteins encoded by these genes. the genes are useful for constructing recombinant host cells having altered fatty acid profiles. oleaginous microalga host cells with the new genes or previously identified fatb genes are disclosed. the microalgae cells produce triglycerides with useful fatty acid profiles. tradução do resumo resumo patente de invenção: "tioesterases e células para a produção de óleos personalizados". a presente invenção apresenta genes acil-acp tioesterase de planta da classe fatb e proteínas codificadas por estes genes. os genes são úteis para construir células hospedeiras recombinantes tendo perfis alterados de ácidos graxos. são reveladas células hospedeiras de microalgas oleaginosas com os novos genes ou genes fatb previamente identificados. as células de microalgas produzem triglicerídeos com perfis de ácidos graxos úteis.
BR112015023192A 2013-03-15 2014-03-13 Tioesterases e células para a produção de óleos personalizados BR112015023192A8 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201361791861P 2013-03-15 2013-03-15
US13/837,996 US9290749B2 (en) 2013-03-15 2013-03-15 Thioesterases and cells for production of tailored oils
US201361917217P 2013-12-17 2013-12-17
PCT/US2014/026644 WO2014151904A1 (en) 2013-03-15 2014-03-13 Thioesterases and cells for production of tailored oils

Publications (2)

Publication Number Publication Date
BR112015023192A2 true BR112015023192A2 (pt) 2017-11-21
BR112015023192A8 BR112015023192A8 (pt) 2018-01-02

Family

ID=51581068

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015023192A BR112015023192A8 (pt) 2013-03-15 2014-03-13 Tioesterases e células para a produção de óleos personalizados

Country Status (9)

Country Link
EP (1) EP2971024A4 (pt)
JP (1) JP2016518112A (pt)
KR (1) KR20150128770A (pt)
CN (1) CN105143458A (pt)
AU (2) AU2014236763B2 (pt)
BR (1) BR112015023192A8 (pt)
CA (1) CA2904395A1 (pt)
MX (1) MX2015011507A (pt)
WO (1) WO2014151904A1 (pt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9816079B2 (en) 2013-01-29 2017-11-14 Terravia Holdings, Inc. Variant thioesterases and methods of use
US9567615B2 (en) 2013-01-29 2017-02-14 Terravia Holdings, Inc. Variant thioesterases and methods of use
US9783836B2 (en) 2013-03-15 2017-10-10 Terravia Holdings, Inc. Thioesterases and cells for production of tailored oils
US9290749B2 (en) 2013-03-15 2016-03-22 Solazyme, Inc. Thioesterases and cells for production of tailored oils
BR112017001404A2 (pt) * 2014-07-24 2017-11-21 Terravia Holdings Inc tioesterases variantes e métodos de utilização
CN107208103A (zh) * 2014-09-18 2017-09-26 泰拉瑞亚控股公司 酰基‑acp硫酯酶及其突变体
US20180142218A1 (en) 2016-10-05 2018-05-24 Terravia Holdings, Inc. Novel acyltransferases, variant thioesterases, and uses thereof
EP4090735A1 (en) 2020-01-16 2022-11-23 Corbion Biotech, Inc. Beta-ketoacyl-acp synthase iv variants

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5512482A (en) 1990-04-26 1996-04-30 Calgene, Inc. Plant thioesterases
US5298421A (en) 1990-04-26 1994-03-29 Calgene, Inc. Plant medium-chain-preferring acyl-ACP thioesterases and related methods
US5344771A (en) 1990-04-26 1994-09-06 Calgene, Inc. Plant thiosterases
US5639790A (en) 1991-05-21 1997-06-17 Calgene, Inc. Plant medium-chain thioesterases
US5455167A (en) * 1991-05-21 1995-10-03 Calgene Inc. Medium-chain thioesterases in plants
US5654495A (en) 1992-10-30 1997-08-05 Calgene, Inc. Production of myristate in plant cells
US5850022A (en) 1992-10-30 1998-12-15 Calgene, Inc. Production of myristate in plant cells
EP0716708A1 (de) * 1993-09-03 1996-06-19 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Mittelkettenspezifische thioesterasen
CA2176137A1 (en) 1993-11-10 1995-05-18 Alois Toni Voelker Plant acyl acp thioesterase sequences
US5807893A (en) 1993-11-18 1998-09-15 Voelker; Toni Alois Plant thioesterases and use for modification of fatty acid composition in plant seed oils
SG182157A1 (en) 2007-06-01 2012-07-30 Solazyme Inc Production of oil in microorganisms
CA3128584A1 (en) * 2007-07-09 2009-01-15 BASF Agricultural Solutions Seed US LLC Brassica mutant fatty acyl-acp thioesterase alleles
US7982035B2 (en) 2007-08-27 2011-07-19 Duquesne University Of The Holy Spirit Tricyclic compounds having antimitotic and/or antitumor activity and methods of use thereof
US20110099669A1 (en) * 2008-06-20 2011-04-28 Basf Plant Science Gmbh Plants having enhanced yield-related traits and a method for making the same
CN102712858B (zh) 2008-11-28 2015-08-12 索拉兹米公司 在重组异养型微生物中制备特制油
CA2758298A1 (en) * 2009-04-10 2010-10-14 Ls9, Inc. Production of commercial biodiesel from genetically modified microorganisms
CN103124499B (zh) 2010-05-28 2016-09-28 泰拉瑞亚控股公司 包含特制油的食品组合物
KR102117225B1 (ko) * 2011-02-02 2020-06-02 테라비아 홀딩스 인코포레이티드 재조합 유지성 미생물로부터 생산된 맞춤 오일
US8951762B2 (en) * 2011-07-27 2015-02-10 Iowa State University Research Foundation, Inc. Materials and methods for using an acyl—acyl carrier protein thioesterase and mutants and chimeras thereof in fatty acid synthesis
CN102586350A (zh) 2012-01-09 2012-07-18 北京化工大学 一种c8:0/c10:0/c12:0/c14:0中链脂肪酸及其乙酯的生产方法
CA2870364A1 (en) 2012-04-18 2013-10-24 Solazyme, Inc. Recombinant microbes with modified fatty acid synthetic pathway enzymes and uses thereof

Also Published As

Publication number Publication date
BR112015023192A8 (pt) 2018-01-02
AU2018267601A1 (en) 2018-12-06
CN105143458A (zh) 2015-12-09
MX2015011507A (es) 2016-04-07
EP2971024A1 (en) 2016-01-20
WO2014151904A1 (en) 2014-09-25
AU2014236763A1 (en) 2015-10-01
EP2971024A4 (en) 2016-11-16
AU2014236763B2 (en) 2018-08-23
CA2904395A1 (en) 2014-09-25
JP2016518112A (ja) 2016-06-23
KR20150128770A (ko) 2015-11-18

Similar Documents

Publication Publication Date Title
BR112015023192A2 (pt) tioesterases e células para a produção de óleos personalizados
BR112017005370A2 (pt) acil-acp tioesterases e mutantes das mesmas
CL2019002663A1 (es) Producción de ácidos grasos poliinsaturados de cadena larga en células vegetales. (divisional solicitud 201702448)
CL2019002477A1 (es) Producción de ácidos graso poliinsaturados de cadena larga en células vegetales. (divisional solicitud 201403402)
EA201600471A1 (ru) Экстрагированный растительный липид, содержащий длинноцепочечные полиненасыщенные жирные кислоты
BR112016006839A8 (pt) Óleos customizados
BR112015016021A2 (pt) processo para a produção de bio - nafta a partir de misturas complexas de gorduras & óleos de ocorrência natural
EA201590251A1 (ru) Новые 5-аминотетрагидрохинолин-2-карбоновые кислоты и их применение
BR112017001404A2 (pt) tioesterases variantes e métodos de utilização
EP3022310A4 (en) Microorganisms and methods for the production of fatty acids and fatty acid derived products
BR112015017920A2 (pt) molécula de ácido nucleico codificando uma proteína transportadore de acila (acp)-acila tioesterase variante, cassete de expressão, vetor, tioesterase (te) variante de proteína transportadora de acila-acila (acp), célula hospedeira, produto de óleo, método para produzir uma planta, alga ou microalga, que produz um óleo com um perfil de ácido graxo desejado, e método para produzir um óleo
BR112012017831A2 (pt) micro-organismos produtores de ácido eicosapentaenóico, composições e ácido graxo e método de fabricação e uso dos mesmos
BR112018005976A2 (pt) óleos de triglicerídeo tendo moléculas de triglicerídeo assimétricas
BR112014018682A8 (pt) Processo para produção de ácidos graxos, sn-regio mono-acilglicerol (mag), sn- regio diacil-gliceróis (dag) e glicerol; e processo para produção de ácidos graxos e glicerol
MA40846A (fr) Compositions d'acides gras et d'esters d'acides gras auto-micellisantes, ainsi que leur utilisation dans le traitement d'états pathologiques
BR112014009333A2 (pt) processo para produzir um éster de ácido graxo epoxidizado a partir de uma gordura ou óleo natural epoxidizado
BR112016000352A8 (pt) produção de produtos a partir de matérias-primas contendo ácidos graxos livres
IL246254A0 (en) Compounds of omega-3 fatty acids for the treatment of diseases involving damage to the nervous system
BR112017020906A2 (pt) microalgas adaptadas para condições de cultura heterotróficas
ITUB20153460A1 (it) Composizioni cosmetiche acquose contenenti esteri dell?acido pelargonico
FR3005317B1 (fr) Levures mutantes capables de produire un acide gras inhabituel
ITUB20153461A1 (it) Composizioni cosmetiche lipofile contenenti esteri dell?acido pelargonico
BR112015018307A2 (pt) utilização de ácido sulfônico para a recuperação de glicerol proveniente da reação de transesterificação de triglicerídeos
WO2014137894A3 (en) Production of omega-3 fatty acids from pythium species
BR112017005959A2 (pt) levedura capaz de produzir etanol a partir de xilose

Legal Events

Date Code Title Description
B25D Requested change of name of applicant approved

Owner name: TERRAVIA HOLDINGS, INC. (US)

B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements