FR3005317B1 - Levures mutantes capables de produire un acide gras inhabituel - Google Patents

Levures mutantes capables de produire un acide gras inhabituel

Info

Publication number
FR3005317B1
FR3005317B1 FR1354062A FR1354062A FR3005317B1 FR 3005317 B1 FR3005317 B1 FR 3005317B1 FR 1354062 A FR1354062 A FR 1354062A FR 1354062 A FR1354062 A FR 1354062A FR 3005317 B1 FR3005317 B1 FR 3005317B1
Authority
FR
France
Prior art keywords
yeas
mutant
fatty acid
unusual fatty
producing unusual
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR1354062A
Other languages
English (en)
Other versions
FR3005317A1 (fr
Inventor
Jean Marc Nicaud
Alain Marty
Athanasios Beopoulos
Jonathan Verbeke
Florence Bordes
Marie Guichard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Institut National de Recherche pour lAgriculture lAlimentation et lEnvironnement
Original Assignee
Centre National de la Recherche Scientifique CNRS
Institut National de la Recherche Agronomique INRA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Institut National de la Recherche Agronomique INRA filed Critical Centre National de la Recherche Scientifique CNRS
Priority to FR1354062A priority Critical patent/FR3005317B1/fr
Priority to EP14727622.4A priority patent/EP2992085A1/fr
Priority to US14/888,605 priority patent/US10415064B2/en
Priority to PCT/IB2014/061116 priority patent/WO2014178014A1/fr
Publication of FR3005317A1 publication Critical patent/FR3005317A1/fr
Application granted granted Critical
Publication of FR3005317B1 publication Critical patent/FR3005317B1/fr
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
FR1354062A 2013-05-02 2013-05-02 Levures mutantes capables de produire un acide gras inhabituel Expired - Fee Related FR3005317B1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
FR1354062A FR3005317B1 (fr) 2013-05-02 2013-05-02 Levures mutantes capables de produire un acide gras inhabituel
EP14727622.4A EP2992085A1 (fr) 2013-05-02 2014-04-30 Levures mutantes capables de produire un acide gras inhabituel
US14/888,605 US10415064B2 (en) 2013-05-02 2014-04-30 Mutant yeasts capable of producing an unusual fatty acid
PCT/IB2014/061116 WO2014178014A1 (fr) 2013-05-02 2014-04-30 Levures mutantes capables de produire un acide gras inhabituel

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR1354062A FR3005317B1 (fr) 2013-05-02 2013-05-02 Levures mutantes capables de produire un acide gras inhabituel

Publications (2)

Publication Number Publication Date
FR3005317A1 FR3005317A1 (fr) 2014-11-07
FR3005317B1 true FR3005317B1 (fr) 2016-03-18

Family

ID=48746023

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1354062A Expired - Fee Related FR3005317B1 (fr) 2013-05-02 2013-05-02 Levures mutantes capables de produire un acide gras inhabituel

Country Status (4)

Country Link
US (1) US10415064B2 (fr)
EP (1) EP2992085A1 (fr)
FR (1) FR3005317B1 (fr)
WO (1) WO2014178014A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3005317B1 (fr) 2013-05-02 2016-03-18 Agronomique Inst Nat Rech Levures mutantes capables de produire un acide gras inhabituel
EP3085788A1 (fr) 2015-04-23 2016-10-26 Institut National De La Recherche Agronomique Souche mutante de levure capable de dégrader la cellobiose
EP3106520A1 (fr) 2015-06-17 2016-12-21 Institut National De La Recherche Agronomique Souche de yarrowia mutante capable de dégrader la galactose
CN109069253B (zh) 2016-04-28 2021-07-06 深圳市科奕顿生物医疗科技有限公司 一种下腔静脉滤器
EP3360956A1 (fr) 2017-02-10 2018-08-15 Institut National De La Recherche Agronomique Souche de levure mutante capable de dégrader la cellobiose

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4937189A (en) 1985-10-18 1990-06-26 Pfizer Inc. Expression and secretion of heterologous proteins by Yarrowia lipolytica transformants
EP0747484A1 (fr) 1995-06-08 1996-12-11 Institut National De La Recherche Agronomique (Inra) Séquences activatrices en amont et séquences promotrices recombinantes fonctionelles dans yarrowia et vecteurs les contenant
DE60036647T2 (de) 2000-04-28 2008-07-17 Laboratoires Mayoly Spindler Klonierung und expression einer extrazellulären säurebeständigen lipase aus yarrowia lipolytica
US8512276B2 (en) 2002-07-24 2013-08-20 Medtronic Minimed, Inc. System for providing blood glucose measurements to an infusion device
US7202356B2 (en) 2003-11-14 2007-04-10 E. I. Du Pont De Nemours And Company Fructose-bisphosphate aldolase regulatory sequences for gene expression in oleaginous yeast
US7264949B2 (en) 2004-09-15 2007-09-04 E.I. Du Pont De Nemours And Company Glycerol-3-phosphate o-acyltransferase promoter for gene expression in oleaginous yeast
US20060094102A1 (en) 2004-11-04 2006-05-04 Zhixiong Xue Ammonium transporter promoter for gene expression in oleaginous yeast
FR2879215B1 (fr) 2004-12-15 2010-08-20 Inst Francais Du Petrole Production d'acides dicarboxyliques par des souches mutantes ameliorees de yarrowia lipolytica
ES2292348B2 (es) 2006-06-08 2008-10-01 Universidade De Santiago De Compostela Metodo y kit de diagnostico precoz y/o pronostico del carcinoma oral de celulas escamosas (coce).
US7923598B2 (en) * 2006-06-22 2011-04-12 Bioriginal Food & Science Corp. Fatty acid hydroxylases and uses thereof
FR2927089B1 (fr) 2008-02-05 2011-03-25 Inst Nat De La Rech Agronomique Inra Procede d'integration ciblee de multicopies d'un gene d'interet dans une souche de yarrowia
ES2575008T3 (es) 2008-07-11 2016-06-23 Nstitut National De La Recherche Agronomique (Inra) Nuevas cepas de levadura mutantes capaces de acumular una gran cantidad de lípidos
US8158391B2 (en) * 2009-05-06 2012-04-17 Dna Twopointo, Inc. Production of an α-carboxyl-ω-hydroxy fatty acid using a genetically modified Candida strain
EP2327776A1 (fr) * 2009-11-30 2011-06-01 Institut National De La Recherche Agronomique Procédé pour la production d'acides gras à chaîne très longue (VLCFA) par fermentation avec un Yarrowia sp recombinant
EP3312271A1 (fr) * 2010-03-02 2018-04-25 Massachusetts Institute Of Technology Ingénierie microbienne pour la production d'acides gras et de dérivés d'acides gras
FR2962133B1 (fr) 2010-07-01 2014-09-12 Agronomique Inst Nat Rech Optimisation de la synthese et de l'accumulation de lipides
FR3002774A1 (fr) 2013-03-04 2014-09-05 Agronomique Inst Nat Rech Levures mutantes ayant une production accrue de lipides et d'acide citrique
FR3005317B1 (fr) 2013-05-02 2016-03-18 Agronomique Inst Nat Rech Levures mutantes capables de produire un acide gras inhabituel

Also Published As

Publication number Publication date
FR3005317A1 (fr) 2014-11-07
EP2992085A1 (fr) 2016-03-09
WO2014178014A1 (fr) 2014-11-06
US10415064B2 (en) 2019-09-17
US20160097066A1 (en) 2016-04-07

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