CL2014003418A1 - Polinucleótido que codifica polipéptido mutante con actividad acil-coa: lisofosfatidilcolina aciltransferasa; célula recombinante que lo comprende; constructo; célula transformada que produce al menos un ácido graso poliinsaturado de cadena larga. - Google Patents

Polinucleótido que codifica polipéptido mutante con actividad acil-coa: lisofosfatidilcolina aciltransferasa; célula recombinante que lo comprende; constructo; célula transformada que produce al menos un ácido graso poliinsaturado de cadena larga.

Info

Publication number
CL2014003418A1
CL2014003418A1 CL2014003418A CL2014003418A CL2014003418A1 CL 2014003418 A1 CL2014003418 A1 CL 2014003418A1 CL 2014003418 A CL2014003418 A CL 2014003418A CL 2014003418 A CL2014003418 A CL 2014003418A CL 2014003418 A1 CL2014003418 A1 CL 2014003418A1
Authority
CL
Chile
Prior art keywords
acyl
construct
produces
fatty acid
polynucleotide encoding
Prior art date
Application number
CL2014003418A
Other languages
English (en)
Inventor
Michael W Bostick
Narendra S Yadav
Hongxiang Zhang
Quinn Qun Zhu
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of CL2014003418A1 publication Critical patent/CL2014003418A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CL2014003418A 2012-06-19 2014-12-17 Polinucleótido que codifica polipéptido mutante con actividad acil-coa: lisofosfatidilcolina aciltransferasa; célula recombinante que lo comprende; constructo; célula transformada que produce al menos un ácido graso poliinsaturado de cadena larga. CL2014003418A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261661623P 2012-06-19 2012-06-19
US201261661615P 2012-06-19 2012-06-19

Publications (1)

Publication Number Publication Date
CL2014003418A1 true CL2014003418A1 (es) 2015-08-28

Family

ID=48670865

Family Applications (2)

Application Number Title Priority Date Filing Date
CL2014003419A CL2014003419A1 (es) 2012-06-19 2014-12-17 Célula microbiana recombinante que sobreexpresa una secuencia que codifica acil-coa: lisofosfatidilcolina aciltranferasa y fosofolípido: diacilglicerol aciltransferasas; y método de producción mejorada de ácidos grasos poliinsaturados.
CL2014003418A CL2014003418A1 (es) 2012-06-19 2014-12-17 Polinucleótido que codifica polipéptido mutante con actividad acil-coa: lisofosfatidilcolina aciltransferasa; célula recombinante que lo comprende; constructo; célula transformada que produce al menos un ácido graso poliinsaturado de cadena larga.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CL2014003419A CL2014003419A1 (es) 2012-06-19 2014-12-17 Célula microbiana recombinante que sobreexpresa una secuencia que codifica acil-coa: lisofosfatidilcolina aciltranferasa y fosofolípido: diacilglicerol aciltransferasas; y método de producción mejorada de ácidos grasos poliinsaturados.

Country Status (8)

Country Link
US (2) US9347075B2 (es)
EP (2) EP2861727B1 (es)
JP (2) JP2015519921A (es)
BR (2) BR112014031827A2 (es)
CA (2) CA2877279A1 (es)
CL (2) CL2014003419A1 (es)
DK (2) DK2861728T3 (es)
WO (2) WO2013192007A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015519921A (ja) * 2012-06-19 2015-07-16 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 突然変異アシルＣｏＡ：リゾホスファチジルコリンアシルトランスフェラーゼ
DK2935601T3 (en) * 2012-12-21 2018-06-18 Du Pont RECOMBINANT MICROBELL CELLS PRODUCING AT LEAST 28% EICOSAPENTAIC ACID AS DRY WEIGHT
JP2017195777A (ja) * 2014-08-29 2017-11-02 国立大学法人京都大学 エイコサペンタエン酸を高含有する脂質の生産方法
CN108330114B (zh) * 2018-03-21 2020-11-03 北京大学 一种利用epa的甘油二酯酰基转移酶及其应用
CN115820586A (zh) * 2022-09-06 2023-03-21 中国农业科学院油料作物研究所 一种溶血磷脂酰胆碱酰基转移酶、核酸分子及其应用

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US5071764A (en) 1983-10-06 1991-12-10 Pfizer Inc. Process for integrative transformation of yarrowia lipolytica
US4880741A (en) 1983-10-06 1989-11-14 Pfizer Inc. Process for transformation of Yarrowia lipolytica
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DE69830587T2 (de) 1997-04-11 2006-05-24 Calgene Llc, Davis Verfahren und zusammensetzungen für die synthese von langkettigen, mehrfach ungesättigten fettsäuren in pflanzen.
CA2366187C (en) * 1999-04-01 2010-05-25 Basf Plant Science Gmbh A new class of enzymes in the biosynthetic pathway for the production of triacylglycerol and recombinant dna molecules encoding these enzymes
TWI377253B (en) 2001-04-16 2012-11-21 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
US20040172682A1 (en) 2003-02-12 2004-09-02 Kinney Anthony J. Production of very long chain polyunsaturated fatty acids in oilseed plants
AU2004215705B2 (en) 2003-02-27 2009-11-12 Basf Plant Science Gmbh Method for the production of polyunsaturated fatty acids
WO2004087902A2 (de) 2003-03-31 2004-10-14 University Of Bristol Neue pflanzliche acyltransferasen spezifisch für langkettige mehrfach ungesättigte fettsäuren
US8313911B2 (en) 2003-05-07 2012-11-20 E I Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7125672B2 (en) 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
US7267976B2 (en) 2003-07-02 2007-09-11 E.I. Du Pont De Nemours And Company Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts
US7550286B2 (en) * 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
DE102004062294A1 (de) 2004-12-23 2006-07-06 Basf Plant Science Gmbh Verfahren zur Herstellung von mehrfach ungesättigten langkettigen Fettsäuren in transgenen Organismen
WO2006076497A2 (en) 2005-01-13 2006-07-20 Novartis Vaccines And Diagnostics Inc. Osplation of pathogenic prions
AU2006227165B2 (en) 2005-03-18 2011-11-10 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
WO2006127655A2 (en) 2005-05-20 2006-11-30 Washington State University Enhancement of hydroxy fatty acid accumulation in oilseed plants
US7732155B2 (en) * 2006-12-13 2010-06-08 National Research Council Of Canada Methods for identifying lysophosphatidylcholine acyltransferases
CA2679988A1 (en) 2007-04-03 2008-10-16 E. I. Du Pont De Nemours And Company Multizymes and their use in making polyunsaturated fatty acids
WO2009001315A2 (en) 2007-06-26 2008-12-31 Staahl Ulf Use of a class of genes encoding lysophospholipid acyl transferases for application in agriculture, biotechnology, and medicine
RU2496881C2 (ru) 2007-07-23 2013-10-27 Сантори Холдингз Лимитед Композиции жирных кислот, имеющие новую пропорцию жирных кислот
CN101970638B (zh) 2007-10-03 2015-02-11 纳幕尔杜邦公司 用于高水平生产二十碳五烯酸的优化解脂耶氏酵母菌株
WO2009129582A1 (en) 2008-04-25 2009-10-29 Commonwealth Scientific Industrial Research Organisation Polypeptides and methods for producing triacylglycerols comprising modified fatty acids
AU2009293160B2 (en) * 2008-09-19 2013-11-21 E. I. Du Pont De Nemours And Company Mutant delta5 desaturases and their use in making polyunsaturated fatty acids
BRPI1009690A2 (pt) * 2009-06-16 2015-08-25 Du Pont "célula hospedeira microbiana oleaginosa, óleo, método de elaboração de um óleo, método de aumento da eficiência de conversão de alongamento de c18 para c20 em uma célula hospedeira recombinante microbiana oleaginosa e método de aumento da eficiência de conversão de dessaturação (delta) 4 em uma célula hospedeira recombinante microbiana oleaginosa produtora de ácidos graxos póli-insaturados de cadeia longa"
CN102803289B (zh) * 2009-06-16 2015-07-22 纳幕尔杜邦公司 用于高水平生产二十碳五烯酸的改善的优化的解脂耶氏酵母菌株
US8524485B2 (en) 2009-06-16 2013-09-03 E I Du Pont De Nemours And Company Long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-CoA lysophospholipid acyltransferases
WO2012027689A1 (en) 2010-08-26 2012-03-01 E. I. Du Pont De Nemours And Company Recombinant microbial host cells for high eicosapentaenoic acid production
JP2015519921A (ja) * 2012-06-19 2015-07-16 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 突然変異アシルＣｏＡ：リゾホスファチジルコリンアシルトランスフェラーゼ

Also Published As

Publication number Publication date
JP2015519920A (ja) 2015-07-16
EP2861727A1 (en) 2015-04-22
WO2013192007A1 (en) 2013-12-27
EP2861728A1 (en) 2015-04-22
DK2861728T3 (en) 2018-03-26
CA2877279A1 (en) 2013-12-27
CL2014003419A1 (es) 2015-08-28
US9416382B2 (en) 2016-08-16
JP2015519921A (ja) 2015-07-16
BR112014031827A2 (pt) 2017-06-27
JP6351582B2 (ja) 2018-07-04
US20130337538A1 (en) 2013-12-19
EP2861727B1 (en) 2018-03-21
CA2877334A1 (en) 2013-12-27
EP2861728B1 (en) 2017-12-27
US9347075B2 (en) 2016-05-24
WO2013192002A1 (en) 2013-12-27
BR112014031784A2 (pt) 2019-10-01
US20130337514A1 (en) 2013-12-19
DK2861727T3 (en) 2018-06-14

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