BR112013002249A2 - meio de cultura de células, métod para obter e/ou cultivar um organóide de fígado, população de células-tronco de fígado adultas ou um organóide de fígado, organóide de fígado, e uso de um organóide de fígado - Google Patents

meio de cultura de células, métod para obter e/ou cultivar um organóide de fígado, população de células-tronco de fígado adultas ou um organóide de fígado, organóide de fígado, e uso de um organóide de fígado

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Publication number
BR112013002249A2
BR112013002249A2 BR112013002249A BR112013002249A BR112013002249A2 BR 112013002249 A2 BR112013002249 A2 BR 112013002249A2 BR 112013002249 A BR112013002249 A BR 112013002249A BR 112013002249 A BR112013002249 A BR 112013002249A BR 112013002249 A2 BR112013002249 A2 BR 112013002249A2
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BR
Brazil
Prior art keywords
liver
liver organoid
organoid
cultivating
obtaining
Prior art date
Application number
BR112013002249A
Other languages
English (en)
Inventor
Johannes Carolus Clevers
Meritxell Huch Ortega
Original Assignee
Koninklijke Nederlandse Akademie Van Wetenschappen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Koninklijke Nederlandse Akademie Van Wetenschappen filed Critical Koninklijke Nederlandse Akademie Van Wetenschappen
Publication of BR112013002249A2 publication Critical patent/BR112013002249A2/pt

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/407—Liver; Hepatocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0672—Stem cells; Progenitor cells; Precursor cells; Oval cells
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/12—Hepatocyte growth factor [HGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
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  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
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  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Epidemiology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Physiology (AREA)
  • Nutrition Science (AREA)
  • Rheumatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

meio de cultura de células, método para obter e/ou cultivar um organóide de fígado, população de células-tronco de fígado adultas ou um organóide de fígado, organóide de fígado, e uso de um organóide de fígado - a invenção refere-se a um organóide de fígado, usos do mesmo, e método para obter os mesmos.
BR112013002249A 2010-07-29 2011-07-29 meio de cultura de células, métod para obter e/ou cultivar um organóide de fígado, população de células-tronco de fígado adultas ou um organóide de fígado, organóide de fígado, e uso de um organóide de fígado BR112013002249A2 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US36873610P 2010-07-29 2010-07-29
EP10171265A EP2412800A1 (en) 2010-07-29 2010-07-29 Liver organoid, uses thereof and culture method for obtaining them
US201161520569P 2011-06-10 2011-06-10
PCT/IB2011/002167 WO2012014076A2 (en) 2010-07-29 2011-07-29 Liver organoid, uses thereof and culture method for obtaining them

Publications (1)

Publication Number Publication Date
BR112013002249A2 true BR112013002249A2 (pt) 2016-05-24

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013002249A BR112013002249A2 (pt) 2010-07-29 2011-07-29 meio de cultura de células, métod para obter e/ou cultivar um organóide de fígado, população de células-tronco de fígado adultas ou um organóide de fígado, organóide de fígado, e uso de um organóide de fígado

Country Status (20)

Country Link
US (3) US9765301B2 (pt)
EP (3) EP2412800A1 (pt)
JP (3) JP6110299B2 (pt)
KR (2) KR102245909B1 (pt)
CN (1) CN103237888B (pt)
AU (3) AU2011284445C1 (pt)
BR (1) BR112013002249A2 (pt)
CA (2) CA2806760C (pt)
DK (1) DK2598633T3 (pt)
ES (1) ES2735248T3 (pt)
HR (1) HRP20191087T1 (pt)
HU (1) HUE044255T2 (pt)
IL (1) IL224409A (pt)
MX (1) MX353579B (pt)
PL (1) PL2598633T3 (pt)
PT (1) PT2598633T (pt)
RU (1) RU2579995C2 (pt)
SG (3) SG10201505887VA (pt)
TR (1) TR201910320T4 (pt)
WO (1) WO2012014076A2 (pt)

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