PT2489743E - Aptâmeros com uridinas substituídas por 5-(n-naftilo) - Google Patents
Aptâmeros com uridinas substituídas por 5-(n-naftilo) Download PDFInfo
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- PT2489743E PT2489743E PT121602999T PT12160299T PT2489743E PT 2489743 E PT2489743 E PT 2489743E PT 121602999 T PT121602999 T PT 121602999T PT 12160299 T PT12160299 T PT 12160299T PT 2489743 E PT2489743 E PT 2489743E
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1048—SELEX
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/16—Aptamers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2310/00—Structure or type of the nucleic acid
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- C12N2310/33—Chemical structure of the base
- C12N2310/334—Modified C
- C12N2310/3341—5-Methylcytosine
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2320/00—Applications; Uses
- C12N2320/10—Applications; Uses in screening processes
- C12N2320/13—Applications; Uses in screening processes in a process of directed evolution, e.g. SELEX, acquiring a new function
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/205—Aptamer
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q2541/00—Reactions characterised by directed evolution
- C12Q2541/10—Reactions characterised by directed evolution the purpose being the selection or design of target specific nucleic acid binding sequences
- C12Q2541/101—Selex
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- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Photometry And Measurement Of Optical Pulse Characteristics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Saccharide Compounds (AREA)
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- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (5)
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| US95028107P | 2007-07-17 | 2007-07-17 | |
| US95028307P | 2007-07-17 | 2007-07-17 | |
| US95029307P | 2007-07-17 | 2007-07-17 | |
| US3142008P | 2008-02-26 | 2008-02-26 | |
| US5159408P | 2008-05-08 | 2008-05-08 |
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| PT2489743E true PT2489743E (pt) | 2015-04-09 |
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| PT121602999T PT2489743E (pt) | 2007-07-17 | 2008-07-17 | Aptâmeros com uridinas substituídas por 5-(n-naftilo) |
| PT90128091T PT2172566E (pt) | 2007-07-17 | 2008-07-17 | Método para gerar aptámeros com taxas de dissociação melhoradas |
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| PT90128091T PT2172566E (pt) | 2007-07-17 | 2008-07-17 | Método para gerar aptámeros com taxas de dissociação melhoradas |
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| US (1) | US8409795B2 (https=) |
| EP (10) | EP2336314A1 (https=) |
| JP (11) | JP5404620B2 (https=) |
| KR (3) | KR101634608B1 (https=) |
| CN (6) | CN101802225B (https=) |
| AU (3) | AU2008275917B2 (https=) |
| CA (4) | CA2693448A1 (https=) |
| DK (4) | DK2172566T4 (https=) |
| ES (6) | ES2647587T3 (https=) |
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| HR (1) | HRP20150382T1 (https=) |
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| PL (1) | PL2489743T3 (https=) |
| PT (2) | PT2489743E (https=) |
| SI (1) | SI2489743T1 (https=) |
| WO (3) | WO2009012418A2 (https=) |
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| JPS5993279A (ja) * | 1982-11-17 | 1984-05-29 | 富士通株式会社 | ロボツトによるカ−ド，レシ−ト分離取出し方法 |
| US20070166741A1 (en) | 1998-12-14 | 2007-07-19 | Somalogic, Incorporated | Multiplexed analyses of test samples |
| US7964356B2 (en) | 2007-01-16 | 2011-06-21 | Somalogic, Inc. | Method for generating aptamers with improved off-rates |
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