MX353340B - Mejoras de cultivos celulares. - Google Patents

Mejoras de cultivos celulares.

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Publication number
MX353340B
MX353340B MX2017003647A MX2017003647A MX353340B MX 353340 B MX353340 B MX 353340B MX 2017003647 A MX2017003647 A MX 2017003647A MX 2017003647 A MX2017003647 A MX 2017003647A MX 353340 B MX353340 B MX 353340B
Authority
MX
Mexico
Prior art keywords
cell culture
mammalian cell
media
culture improvements
improved
Prior art date
Application number
MX2017003647A
Other languages
English (en)
Inventor
A Pla Itzcoatl
C Matuck Joseph
C Fann John
Schulz Christof
A Roy Nicole
F Bruton David
Mcintire James
Hsiang David CHANG Yu-
Seewoester Thomas
Original Assignee
Abbvie Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=39184386&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX353340(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Abbvie Inc filed Critical Abbvie Inc
Publication of MX353340B publication Critical patent/MX353340B/es

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
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    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2869—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against hormone receptors
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0031—Serum-free culture media
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C07K2317/00—Immunoglobulins specific features
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    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/74—Undefined extracts from fungi, e.g. yeasts
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    • C—CHEMISTRY; METALLURGY
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin
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    • C12N2511/00—Cells for large scale production

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Endocrinology (AREA)
  • Neurology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

La presente invención se refiere a métodos mejorados para producir una proteína recombinante, por ejemplo un anticuerpo, en un cultivo de células de mamífero. Además, la invención proporciona medios de cultivo mejorados, incluyendo medios de producción mejorados, soluciones de alimentación y soluciones combinadas, que se pueden utilizar para mejorar la productividad de proteínas en cultivos de células de mamífero. Un método de producción de lote alimentado para producir adalimumab, que comprende cultivar células de Ovario de Hámster Chino /CHO) que comprenden un ácido nucleico que codifica adalumimab en un medio de producción de cultivo celular a gran escala, en donde se monitorea la concentración de glucosa en el medio, la concentración de glucosa en el medio disminuye por debajo de 2 g/L, y se añade glucosa al medio cuando la concentración de glucosa en el medio disminuye por debajo de 2 g/L, o se monitorea la concentración de glucosa en el medio y se añade glucosa al medio para mantener la concentración de glucosa en el medio a una concentración de al menos 2 g/L pero no mayor de 7 g/L, de manera que se produce adalimumab, y en donde el adalimumab producido se purifica adicionalmente por afinidad utilizando una resina de proteína A.
MX2017003647A 2006-09-13 2007-09-13 Mejoras de cultivos celulares. MX353340B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US84515806P 2006-09-13 2006-09-13
US87637406P 2006-12-21 2006-12-21
PCT/US2007/020027 WO2008033517A2 (en) 2006-09-13 2007-09-13 Cell culture improvements

Publications (1)

Publication Number Publication Date
MX353340B true MX353340B (es) 2018-01-09

Family

ID=39184386

Family Applications (4)

Application Number Title Priority Date Filing Date
MX2009002748A MX2009002748A (es) 2006-09-13 2007-09-13 Mejoras de cultivos celulares.
MX2016013989A MX346523B (es) 2006-09-13 2007-09-13 Mejoras de cultivos celulares.
MX2017003647A MX353340B (es) 2006-09-13 2007-09-13 Mejoras de cultivos celulares.
MX2012004477A MX345141B (es) 2006-09-13 2007-09-13 Mejoras de cultivos celulares.

Family Applications Before (2)

Application Number Title Priority Date Filing Date
MX2009002748A MX2009002748A (es) 2006-09-13 2007-09-13 Mejoras de cultivos celulares.
MX2016013989A MX346523B (es) 2006-09-13 2007-09-13 Mejoras de cultivos celulares.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2012004477A MX345141B (es) 2006-09-13 2007-09-13 Mejoras de cultivos celulares.

Country Status (17)

Country Link
US (11) US8093045B2 (es)
EP (4) EP2500413A1 (es)
JP (3) JP5878682B2 (es)
KR (2) KR20090074040A (es)
CN (6) CN101663390B (es)
AU (1) AU2007294731B2 (es)
BR (1) BRPI0716762A2 (es)
CA (5) CA2663442A1 (es)
IL (1) IL197444A0 (es)
MX (4) MX2009002748A (es)
MY (4) MY185887A (es)
NO (1) NO20091439L (es)
NZ (2) NZ575328A (es)
RU (2) RU2518289C2 (es)
SG (3) SG192441A1 (es)
TW (4) TWI548747B (es)
WO (1) WO2008033517A2 (es)

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