GB2452456A - Cell growth medium - Google Patents

Cell growth medium

Info

Publication number
GB2452456A
GB2452456A GB0823575A GB0823575A GB2452456A GB 2452456 A GB2452456 A GB 2452456A GB 0823575 A GB0823575 A GB 0823575A GB 0823575 A GB0823575 A GB 0823575A GB 2452456 A GB2452456 A GB 2452456A
Authority
GB
United Kingdom
Prior art keywords
cell growth
growth medium
feeder
stem cells
relates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB0823575A
Other versions
GB0823575D0 (en
Inventor
Tesuji Okamoto
Denry Sato
Miho Furue
Peter Andrews
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Sheffield
Original Assignee
University of Sheffield
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Sheffield filed Critical University of Sheffield
Publication of GB0823575D0 publication Critical patent/GB0823575D0/en
Publication of GB2452456A publication Critical patent/GB2452456A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin

Abstract

The invention relates to a method to culture primate embryonic stem cells in feeder and serum free conditions.
GB0823575A 2006-07-12 2008-12-29 Cell growth medium Withdrawn GB2452456A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0613756.6A GB0613756D0 (en) 2006-07-12 2006-07-12 Cell culture medium
PCT/GB2007/002584 WO2008007082A2 (en) 2006-07-12 2007-07-10 Cell growth medium

Publications (2)

Publication Number Publication Date
GB0823575D0 GB0823575D0 (en) 2009-01-28
GB2452456A true GB2452456A (en) 2009-03-04

Family

ID=36955441

Family Applications (2)

Application Number Title Priority Date Filing Date
GBGB0613756.6A Ceased GB0613756D0 (en) 2006-07-12 2006-07-12 Cell culture medium
GB0823575A Withdrawn GB2452456A (en) 2006-07-12 2008-12-29 Cell growth medium

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GBGB0613756.6A Ceased GB0613756D0 (en) 2006-07-12 2006-07-12 Cell culture medium

Country Status (7)

Country Link
EP (1) EP2038404A2 (en)
JP (1) JP5227318B2 (en)
CN (1) CN101490243A (en)
AU (1) AU2007274065A1 (en)
CA (1) CA2657539A1 (en)
GB (2) GB0613756D0 (en)
WO (1) WO2008007082A2 (en)

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EP1962719A4 (en) 2005-08-29 2011-05-04 Technion Res And Dev Of Foundation Ltd Media for culturing stem cells
ES2874223T3 (en) 2006-08-02 2021-11-04 Technion Res & Dev Foundation Embryonic stem cell expansion methods in suspension culture
US9688956B2 (en) 2008-06-05 2017-06-27 National Cheng Kung University Method for preserving proliferation and differentiation potential of mesenchymal stem cells
EP2303183A4 (en) 2008-06-24 2015-11-04 Bioactive Surgical Inc Surgical sutures incorporated with stem cells or other bioactive materials
WO2010017459A1 (en) 2008-08-07 2010-02-11 Bioactive Surgical, Inc> Stem cell capture and immobilization coatings for medical devices and implants
BRPI0922572A2 (en) * 2008-12-17 2019-09-24 Scripps Research Inst method for culturing pluripotent cells, pluripotent mammalian cell culture, cell culture medium, isolated pluripotent animal cell, and method for increasing pluripotence of a mammalian cell.
AU2010275764A1 (en) * 2009-07-21 2012-03-15 Transgene Sa Enzymatic composition for the digestion of chicken embryos
CA3080762A1 (en) * 2009-11-12 2011-05-19 Technion Research & Development Foundation Limited Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
CN101812480B (en) * 2009-11-16 2013-04-10 西北农林科技大学 Method utilizing transcription factor to transfect bovine body cell into induced pluripotent stem cell
JP5714267B2 (en) * 2010-08-26 2015-05-07 日本メナード化粧品株式会社 Stem cell undifferentiation maintenance agent and proliferation promoter
JP6148429B2 (en) * 2011-01-31 2017-06-14 協和発酵バイオ株式会社 Method for culturing human pluripotent stem cells
CN102586176B (en) * 2012-01-11 2013-05-08 中国科学院生物物理研究所 Novel animal source-free and feed layer-free human pluripotent stem cell culture system
GB201211873D0 (en) * 2012-07-04 2012-08-15 Univ Edinburgh Cell culture
JP6380109B2 (en) 2013-01-31 2018-08-29 味の素株式会社 Cultivation method for stable undifferentiated maintenance proliferation of pluripotent stem cells
EP3006558B1 (en) * 2013-05-30 2020-05-13 Ajinomoto Co., Inc. Medium for culturing stem cells
EP3015551B1 (en) * 2013-06-28 2019-03-06 Kaneka Corporation Method for screening for pluripotent stem cell growth-promoting factor
WO2015042356A1 (en) * 2013-09-19 2015-03-26 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Chemically defined culture medium for stem cell maintenance and differentiation
CN104357379B (en) * 2014-09-30 2017-08-08 刘兴宇 Stem cell media
CN106032527B (en) * 2015-03-17 2019-08-13 广州市搏克肿瘤研究所 It is a kind of tolerance low-density without feeder layer human pluripotent stem cells culture medium
CN105907705B (en) * 2016-06-28 2020-01-24 广州市搏克生物技术有限公司 Pluripotent stem cell culture medium
AU2018209127B2 (en) 2017-01-23 2021-10-21 Stemcell Technologies Canada Inc. Media and methods for enhancing the survival and proliferation of stem cells
CN106754715A (en) * 2017-02-13 2017-05-31 四川新生命干细胞科技股份有限公司 A kind of trophoblastic preparation method for candidate stem cell culture
CN106754652B (en) * 2017-03-06 2019-04-02 广州润虹医药科技股份有限公司 IPS cell differentiation at ectoderm progenitor cells serum-free induced medium and abductive approach
CN106754657B (en) * 2017-03-28 2022-07-22 北京赛斯达生物技术有限公司 Serum-free medium for monkey embryonic stem cells
DK3436568T3 (en) * 2017-05-31 2023-09-18 Promocell Gmbh CULTURE MEDIUM FOR PLURIPOTENT STEM CELLS
CN110157662A (en) * 2018-02-15 2019-08-23 郭永珍 A kind of cell additive and preparation method thereof
KR102147780B1 (en) * 2018-03-16 2020-08-25 사회복지법인 삼성생명공익재단 Culture medium composition for promoting the proliferation of stem cell comprising FGF-17 as an effective ingredient and method of culturing stem cells using the same
WO2020004571A1 (en) * 2018-06-27 2020-01-02 味の素株式会社 Additive for culturing stem cells, culturing medium, and culturing method
CN110684716A (en) * 2018-07-08 2020-01-14 郭永珍 Preparation method of cell additive and product thereof
CN109486766B (en) * 2018-11-26 2021-05-07 中山大学 Lacrimal gland stem cell, culture system and culture method of lacrimal gland stem cell
CN111484970B (en) * 2020-04-30 2022-09-16 广州再生医学与健康广东省实验室 Serum-free and feeder-layer-free embryo and pluripotent stem cell culture medium with low protein content
WO2023150293A2 (en) * 2022-02-03 2023-08-10 Steakholder Foods Ltd. Accelerated myotube formation

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998030679A1 (en) * 1997-01-10 1998-07-16 Life Technologies, Inc. Embryonic stem cell serum replacement
WO2003029443A1 (en) * 2001-09-28 2003-04-10 Es Cell International Pte Ltd Methods of derivation and propagation of undifferentiated human embryonic stem (hes) cells on feeder-free matrices and human feeder layers
WO2004111210A1 (en) * 2003-06-12 2004-12-23 Yeda Research & Development Co.Ltd Enhancement of oligodendrocyte differentiation
WO2006017370A2 (en) * 2004-07-13 2006-02-16 Geron Corporation Medium for growing human embryonic stem cells
WO2006029197A1 (en) * 2004-09-08 2006-03-16 Wisconsin Alumni Research Foundation Medium and culture of embryonic stem cells
WO2006036925A1 (en) * 2004-09-28 2006-04-06 Wicell Research Institute, Inc. Cultivation of primate embryonic stem cells

Family Cites Families (1)

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Publication number Priority date Publication date Assignee Title
DE602004026917D1 (en) * 2003-12-26 2010-06-10 Makoto Asashima BASE MEDIUM FOR CULTIVATING ES CELLS

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998030679A1 (en) * 1997-01-10 1998-07-16 Life Technologies, Inc. Embryonic stem cell serum replacement
WO2003029443A1 (en) * 2001-09-28 2003-04-10 Es Cell International Pte Ltd Methods of derivation and propagation of undifferentiated human embryonic stem (hes) cells on feeder-free matrices and human feeder layers
WO2004111210A1 (en) * 2003-06-12 2004-12-23 Yeda Research & Development Co.Ltd Enhancement of oligodendrocyte differentiation
WO2006017370A2 (en) * 2004-07-13 2006-02-16 Geron Corporation Medium for growing human embryonic stem cells
WO2006029197A1 (en) * 2004-09-08 2006-03-16 Wisconsin Alumni Research Foundation Medium and culture of embryonic stem cells
WO2006036925A1 (en) * 2004-09-28 2006-04-06 Wicell Research Institute, Inc. Cultivation of primate embryonic stem cells

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BIOTECHNOLOGY AND BIOENGINEERING, Vol. 91, 2005, Y Li et al, "Expansion of human embryonic stem cells in defined serum-free medium devoid of animal-derived products" , 688-698 *
EXPERIMENTAL NEUROLOGY, Vol. 152, 1998, MA Caldwell et al, "Heparin, but not other proteoglycans potentiates the mitogenic effects of FGF-2 on mesencephalic precursor wells", 1-10 *
NATURE BIOTECHNOLOGY, Vol. 19, 2001, S-C Zhang et al, "In vitro differentiation of transplantable neural precursors from human embryonic stem cells", 1129-1133, *
NEUROLOGICAL RESEARCH, Vol. 28, 2006, W Ren et al, "bFGF and heparin but not laminin are necessary factors in the mediums that affect NSCs differentiation into cholinergic neurons.", 87-90 *

Also Published As

Publication number Publication date
WO2008007082A2 (en) 2008-01-17
GB0613756D0 (en) 2006-08-23
WO2008007082A3 (en) 2008-03-06
JP2009542247A (en) 2009-12-03
CN101490243A (en) 2009-07-22
CA2657539A1 (en) 2008-01-17
AU2007274065A1 (en) 2008-01-17
EP2038404A2 (en) 2009-03-25
JP5227318B2 (en) 2013-07-03
GB0823575D0 (en) 2009-01-28

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Legal Events

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WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)